CN105861307B - Suitable for industrial applications can magnetic recovery biological enzyme process units - Google Patents
Suitable for industrial applications can magnetic recovery biological enzyme process units Download PDFInfo
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- CN105861307B CN105861307B CN201610321556.XA CN201610321556A CN105861307B CN 105861307 B CN105861307 B CN 105861307B CN 201610321556 A CN201610321556 A CN 201610321556A CN 105861307 B CN105861307 B CN 105861307B
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- service sink
- separating still
- pipeline
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- reaction kettle
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/14—Bioreactors or fermenters specially adapted for specific uses for producing enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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- Wood Science & Technology (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The invention discloses it is a kind of suitable for industrial applications can magnetic recovery biological enzyme process units, including reaction kettle, the autoclave body top is equipped with feed(raw material)inlet, recycling biological enzyme solutions entrance, the outlet of mixed liquor is arranged at lower part, outlet is connected with separating still, the lower part of separating still has pipeline to be connected with reaction kettle by plastic pump, while another outlet is connected by plastic pump with clear water reserviors.The lower part of separating still and clear water reserviors all there are one strong electromagnet, generated after energization strong magnetic to obtain can magnetic recovery biological enzyme agent carry out magnetic force recycling, achieve the purpose that quick separating, catalyst and liquid made to detach quickening processing.The top of clear water reserviors by pipeline and plastic pump and separating still be connected.The device that the present invention designs can be used to can magnetic force recycling catalyst itself industrialized production, it can also be used to industry in using can magnetic force recycling, reusable catalyst other industrial installations.
Description
Technical field
The invention belongs to catalyst production technical fields, and in particular to it is a kind of suitable for industrial applications can magnetic recovery
The process units of biological enzyme.
Background technology
Enzyme has that high selectivity, catalytic condition be mild, the distinguishing features such as pollution-free as biocatalyst, is opened
Hair is widely used in the industries such as food processing, medicine, weaving and fine chemistry industry.The use of enzyme is typically under free state with dilute
The form of solution carries out catalysis with substrate and reacts, and is difficult to recycle enzyme after the completion of reaction, is only preferably used in tank reactor, point
It criticizes and launches, cost is higher.Natural enzyme stability is poor simultaneously, and easy in inactivation cannot be reused, and product isolates and purifies difficulty,
Make it difficult to the large-scale application in Industrial Catalysis.In recent years, stability and the reuse of enzyme are improved by immobilization
Number becomes the hot spot of research.Enzyme is combined with certain insoluble macromolecular carriers, immobilised enzymes is formed, its advantage is that can be with structure
At working continuously, can be used in the stirred reactor, fixed bed reactors or fluidized-bed reactor continuously flowed.Immobilization
The stability of enzyme often can be improved afterwards, vigor also can be improved in some occasions.But the recycling of supported biological enzyme agent
The problem of how being detached from reaction system is still suffered from, often along with the unit operations such as filtering or centrifugation, leads to biology
The loss of enzyme even inactivates, and regeneration effect causes anxiety.
Nano magnetic material is as ideal biological zymophore, with the performance not available for conventional material so that it is latent
Application it is extremely advantageous, for nano magnetic material and its immobilized application aspect research work of biological enzyme oneself through no longer limiting to
In single subject and single research method, but the comprehensive utilization of multidisciplinary and a variety of research methods.By to magnetic particles sublist
Face functionalization, chemical coupling effect occurs with biological enzyme after functionalization keeps biological enzyme firm immobilized on magnetic carrier, magnetic
Carrier is with its efficient magnetic action, convenient for recycling after use.Although have at present can magnetic recovery biological enzyme small-sized preparation
Device, but since biological enzyme is the bioactive substances such as protein or polypeptide, stability is poor, easy in inactivation, so the device is only
It is limited in the preparation of laboratory or a small amount of biological enzyme, there is presently no the devices suitable for industrialized production or recycling biological enzyme.
Invention content
The purpose of the present invention be exactly to solve the above-mentioned problems, provide it is a kind of suitable for industrial applications can magnetic recovery
The process units of biological enzyme, can effectively prepare can the immobilized biological enzyme agent of magnetic recovery.
To achieve the goals above, the present invention adopts the following technical scheme that：
It is a kind of suitable for industrial applications can magnetic recovery biological enzyme process units, including polytetrafluoroethyl-ne alkene reaction
Kettle, separating still and service sink,
The ptfe autoclave top setting feed(raw material)inlet and first pipe, the ptfe autoclave are logical
The first pipe is crossed to connect with the separating still side wall, the ptfe autoclave lower part by second pipe with detach
Kettle top portion connects, and blender is arranged inside the ptfe autoclave；
Electromagnet is arranged in the separating still bottom, and the separating still lower part at the top of the 4th pipeline and the service sink by connecting
It connects, the top of the separating still is connected by the side wall of third pipeline and service sink；
Clear water entrance is set at the top of the service sink, and wastewater outlet, the service sink is arranged in the service sink upper portion side wall
Products export is arranged in lower part, and the service sink bottom is arranged electromagnet, blender is arranged in the service sink；
Valve is all set on first, second, third and fourth pipeline, described first and third, plastic pump is all set on four pipelines.
It is preferred that：Service sink lower part setting products export is connect by the 5th pipeline with pan tank.
It is preferred that：The top of the separating still is arranged upper cover, on the upper lid with second and third pipeline by hole.
It is preferred that：The pipeline is high-performance PVC (polyvinyl chloride) pipe.
It is preferred that：The shell of the reaction kettle is supported on holder.
It is preferred that：The service sink is rectangular, and upper cover is arranged in the top of the service sink, has the 4th pipeline on the upper lid
By hole and clear water entrance.
It is preferred that：The reaction kettle, separating still and service sink use polytetrafluoroethylene material, advantage：It effectively prevent magnetic green
The attachment of object enzyme catalyst increases the fluency of conveying.
Separating still and clear water reserviors top carry cover board, prevent liquid spluttering and other foreign matters from entering inside.
Above-mentioned device can be in magnetic recovery material in production application.
A method of based on above-mentioned apparatus industrialized production can magnetic recovery biological enzyme,
(1) finished product magnetic carrier and coupling agent are added in reaction kettle, add biological enzyme solutions, it is anti-by stirring
It answers；
(2) after the completion of reacting, the valve on the second pipe of reaction kettle is opened, the mixing liquid after reaction is made to enter separation
Kettle；
(3) magnet of the reactor bottom is powered, so that the magnetic bio enzyme in mixed liquor is attracted, makes magnetic bio
Enzyme is detached with solution, and the liquid after separation is pumped into reaction kettle, isolated magnetism by the plastic pump in the first pipe
Biological enzyme is pumped into service sink by the pump on the 4th pipeline；
(4) clear water is pumped into service sink, stirring and washing will distinguish water pump by the pump on the 4th pipeline in the middle part of service sink
Enter in separating still, the magnetic bio enzyme catalyst in separating still is made to be transferred into clear water reserviors completely；
(5) magnet of the service sink bottom is powered, the waste water after cleaning is discharged.
It is preferred that：The method, which further includes the magnetic bio enzyme after (6) cleaning, has pump to squeeze into pan tank.
Beneficial effects of the present invention：
The process units of the present invention can be used for industrialized production can magnetic force recycling catalyst and carrier material
Large-scale plant, may be implemented Kg even tonne other production, can efficiently realize can magnetic force recycling catalyst quick separation,
It is easy to maintenance with simple in structure, small investment, it is ensured that run well for a long time.Magnetic bio can be efficiently prepared on a large scale
Enzyme catalyst.When for industrialized production, it is immobilized after enzyme solutions can apply mechanically, realize that cycle reduces waste, so with very
Good economy.
Pump used in the present invention is all plastic pump, material by part be all made of polytetrafluoroethylplastic plastic.
Magnetic bio enzyme catalyst is reduced in adherency above.
The bottom of service sink also is provided with a strong electromagnet, the convenient magnetic force to the magnetic bio enzyme in cleaning process point
From recycling, the loss in cleaning process is reduced.
Description of the drawings
Fig. 1 is the structural schematic diagram of process units of the present invention；
Wherein, 1. reaction kettle, 2. separating stills, 3. electromagnet, 4. first pipes, 5. second pipes, 6. clear water reserviors, 7. the 4th
Pipeline, 8. third pipelines, 9. clear water entrances, 10. wastewater outlets, 11. the 5th pipelines, 12. products exports, 13. blenders.
Specific implementation mode
The invention will be further described with embodiment below in conjunction with the accompanying drawings.
As shown in Figure 1, it is a kind of suitable for industrial applications can magnetic recovery biological enzyme process units, including polytetrafluoro
Feed(raw material)inlet and first pipe is arranged in ethylene reaction kettle 1, separating still 2 and service sink 6,1 top of the ptfe autoclave
4, the ptfe autoclave 1 is connect by the first pipe 4 with 2 side wall of the separating still, the polytetrafluoroethylene (PTFE)
1 lower part of reaction kettle is connect by second pipe 5 with 2 top of separating still, and blender is arranged in 1 inside of the ptfe autoclave
13；
Electromagnet 3 is arranged in 2 bottom of the separating still, and 2 lower part of the separating still passes through the 4th pipeline 7 and the service sink 6
Top connects, and the top of the separating still 2 is connect by third pipeline 8 with the side wall of service sink 6；
6 top setting clear water entrance 9 of the service sink, wastewater outlet 10 is arranged in 6 upper portion side wall of the service sink, described clear
Products export 12 is arranged in 6 lower part of wash pool, and 6 bottom of the service sink is arranged electromagnet 3, blender 13 is arranged in the service sink 6；
Valve is all set on first, second, third and fourth pipeline, described first and third, plastic pump is all set on four pipelines.
6 lower part of the service sink setting products export 12 is connect by the 5th pipeline 11 with pan tank.
The top of the separating still 2 is arranged upper cover, on the upper lid with second and third pipeline by hole.
The pipeline is high-performance PVC (polyvinyl chloride) pipe.
The shell of the reaction kettle is supported on holder.
The service sink 6 is rectangular, and upper cover is arranged in the top of the service sink 6, has the 4th pipeline 7 logical on the upper lid
The hole crossed and clear water entrance 9.
The reaction kettle 1, separating still 2 and service sink 6 use polytetrafluoroethylene material.
The course of work of above-mentioned apparatus is as follows：
In use, finished product magnetic carrier is dissolved in buffer solution, certain density coupling agent and certain density is prepared
Biological enzyme is sent by feed(raw material)inlet in reaction kettle 1, is started blender 13 and is started to react.The regular hour is reacted, after reaction
During mixing liquid is entered by second pipe 52 in separating still.The power supply for opening the electromagnet 3 of 2 bottom of separating still, makes electromagnet 3
Energizing magnetic makes the magnetic bio enzyme catalyst in mixing liquid be detached with unreacted biological enzyme solutions, after separation
Biological enzyme solutions are squeezed by plastic pump in reaction kettle 1, and the biological enzyme agent that bottom is adsorbed is powered in stopping electromagnet 3
Afterwards, it is squeezed into clear water reserviors 6 by plastic pump.Isolated magnetic bio enzyme catalyst is clear in clear water reserviors 6 in separating still 2
It washes, while part clear water being sent into separating still 2 by plastic pump, cleaning part remains in the magnetic bio enzyme in separating still 2
Catalyst, cleaning makes magnetic bio enzyme catalyst all be transferred in service sink 6 three times, after cleaning, opens the electricity of 6 bottom of service sink
The power supply of magnet 3 makes electromagnet 3 generate strong magnetic, the biological enzyme agent attracted.Supernatant is beaten by plastic pump
It returns in separating still 2, turnover.The magnetic bio enzyme catalyst of bottom after the magnetism for removing electromagnet 3 by being pumped into pan tank, into
Row subsequent processing obtains finished product.
Above-mentioned, although the foregoing specific embodiments of the present invention is described with reference to the accompanying drawings, not protects model to the present invention
The limitation enclosed, those skilled in the art should understand that, based on the technical solutions of the present invention, those skilled in the art are not
Need to make the creative labor the various modifications or changes that can be made still within protection scope of the present invention.
Claims (8)
1. it is a kind of suitable for industrial applications can magnetic recovery biological enzyme process units, it is characterized in that：Including polytetrafluoroethyl-ne
Alkene reaction kettle, separating still and service sink；The ptfe autoclave top setting feed(raw material)inlet and first pipe, it is described poly-
Tetrafluoroethene reaction kettle is connect by the first pipe with the separating still side wall, and the ptfe autoclave lower part is logical
It crosses at the top of second pipe and separating still and connect, blender is set inside the ptfe autoclave；The separating still bottom
Electromagnet is set, and by being connect at the top of the 4th pipeline and the service sink, the top of the separating still is logical for the separating still lower part
The side wall for crossing third pipeline and service sink connects；Clear water entrance is set at the top of the service sink, and the service sink upper portion side wall is set
Wastewater outlet is set, products export is arranged in the service sink lower part, and the service sink bottom is arranged electromagnet, is set in the service sink
Set blender；Valve is all set on first, second, third and fourth pipeline, described first and third, plastic pump is all set on four pipelines；
The process units carry out industrialized production can magnetic recovery biological enzyme method, steps are as follows：
(1) finished product magnetic carrier and coupling agent are added in reaction kettle, biological enzyme solutions are added, by being stirred to react；
(2) after the completion of reacting, the valve on the second pipe of reaction kettle is opened, the mixing liquid after reaction is made to enter separating still；
(3) by the magnet of the separating still bottom be powered, so that the magnetic bio enzyme in mixed liquor is attracted, make magnetic bio enzyme with
Solution detaches, and the liquid after separation is pumped into reaction kettle, isolated magnetic bio by the plastic pump in the first pipe
Enzyme is pumped into service sink by the pump on the 4th pipeline；
(4) it is pumped into clear water into service sink, stirring and washing will distinguish water in the middle part of service sink by the pump on the 4th pipeline and be pumped into point
It is transferred into service sink completely from the magnetic bio enzyme catalyst in separating still in kettle, is made；
(5) magnet of the service sink bottom is powered, the waste water after cleaning is discharged.
2. device as described in claim 1, it is characterized in that：Service sink lower part setting products export by the 5th pipeline with
Pan tank connects.
3. device as described in claim 1, it is characterized in that：Upper cover is arranged in the top of the separating still, has on the upper lid
Second and third pipeline by hole.
4. device as described in claim 1, it is characterized in that：The shell of the reaction kettle is supported on holder.
5. device as described in claim 1, it is characterized in that：The service sink is rectangular, in the top setting of the service sink
Lid, on the upper lid with the 4th pipeline by hole and clear water entrance.
6. device as described in claim 1, it is characterized in that：The reaction kettle, separating still and service sink use polytetrafluoroethylene (PTFE)
Material.
7. device as described in claim 1, it is characterized in that：The pipeline is high-performance PVC (polyvinyl chloride) pipe.
8. device as described in claim 1, it is characterized in that：The method further include (6) cleaning after magnetic bio enzyme by pumping
Squeeze into pan tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610321556.XA CN105861307B (en) | 2016-05-13 | 2016-05-13 | Suitable for industrial applications can magnetic recovery biological enzyme process units |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610321556.XA CN105861307B (en) | 2016-05-13 | 2016-05-13 | Suitable for industrial applications can magnetic recovery biological enzyme process units |
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| Publication Number | Publication Date |
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| CN105861307A CN105861307A (en) | 2016-08-17 |
| CN105861307B true CN105861307B (en) | 2018-08-28 |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2611040Y (en) * | 2003-04-22 | 2004-04-14 | 樊福生 | On-line equipment for magnetic separation and recovery of catalyst |
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| CN101143347A (en) * | 2007-11-06 | 2008-03-19 | 史和龙 | Magnetic catalyst granule continuous in-situ separating device and operation method |
| CN101280298A (en) * | 2008-05-29 | 2008-10-08 | 山东大学 | Preparation of recyclable magnetic nanometer immobilized enzyme |
| CN101654655A (en) * | 2009-09-03 | 2010-02-24 | 华中科技大学 | Bioreactor applied to magnetic nano-catalyst |
| JP2010131005A (en) * | 2008-10-30 | 2010-06-17 | Contig I:Kk | Immobilized biocatalyst and its production method |
| CN102351654A (en) * | 2011-06-15 | 2012-02-15 | 中北大学 | Magnetic separation catalytic oxidation method and magnetic separation reaction apparatus |
| CN202626197U (en) * | 2012-07-13 | 2012-12-26 | 青岛农业大学 | Special enzymolysis tank for magnetic immobilized enzyme |
| CN205590711U (en) * | 2016-05-13 | 2016-09-21 | 山东师范大学 | But apparatus for producing of biological enzyme is retrieved to magnetism suitable for industrialization is used |
-
2016
- 2016-05-13 CN CN201610321556.XA patent/CN105861307B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2611040Y (en) * | 2003-04-22 | 2004-04-14 | 樊福生 | On-line equipment for magnetic separation and recovery of catalyst |
| CN1721027A (en) * | 2005-05-18 | 2006-01-18 | 戴群 | Integrated enzyme membrane circulation extracting process and apparatus therefor |
| CN101143347A (en) * | 2007-11-06 | 2008-03-19 | 史和龙 | Magnetic catalyst granule continuous in-situ separating device and operation method |
| CN101280298A (en) * | 2008-05-29 | 2008-10-08 | 山东大学 | Preparation of recyclable magnetic nanometer immobilized enzyme |
| JP2010131005A (en) * | 2008-10-30 | 2010-06-17 | Contig I:Kk | Immobilized biocatalyst and its production method |
| CN101654655A (en) * | 2009-09-03 | 2010-02-24 | 华中科技大学 | Bioreactor applied to magnetic nano-catalyst |
| CN102351654A (en) * | 2011-06-15 | 2012-02-15 | 中北大学 | Magnetic separation catalytic oxidation method and magnetic separation reaction apparatus |
| CN202626197U (en) * | 2012-07-13 | 2012-12-26 | 青岛农业大学 | Special enzymolysis tank for magnetic immobilized enzyme |
| CN205590711U (en) * | 2016-05-13 | 2016-09-21 | 山东师范大学 | But apparatus for producing of biological enzyme is retrieved to magnetism suitable for industrialization is used |
Non-Patent Citations (2)
| Title |
|---|
| Immobilized cellulase on Fe3O4 nanoparticles as a magnetically recoverable biocatalyst for the decomposition of corncob;Qikun Zhang等;《Chinese Journal of Catalysis》;20160305;第37卷(第3期);第390页右栏第3段至最后1段 * |
| 可磁力再生生物酶催化剂的制备与应用;赵雷振;《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》;20150915(第09期);第B014-204页 * |
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