CN202576417U - Low-shear force aeration conical flask - Google Patents
Low-shear force aeration conical flask Download PDFInfo
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- CN202576417U CN202576417U CN 201220167506 CN201220167506U CN202576417U CN 202576417 U CN202576417 U CN 202576417U CN 201220167506 CN201220167506 CN 201220167506 CN 201220167506 U CN201220167506 U CN 201220167506U CN 202576417 U CN202576417 U CN 202576417U
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- triangular flask
- bottleneck
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
- C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- Wood Science & Technology (AREA)
- Microbiology (AREA)
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- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model belongs to the field of microbial culture, and specifically relates to a novel conical flask capable of improving the oxygen dissolution efficiency of microorganisms. In a low-shear force aeration conical flask, separation blades parallel to the bottom surface are formed in the positions close to the bottom surface of a flask body; and 4, 6 or 8 separation blades are uniformly distributed along the periphery of the flask body. The separation blades close to the bottom surface of the flask body of the conical flask are favorable for increasing the contact probability and contact area between a culture medium and the sterile air when the culture medium moves along with the conical flask, thus more oxygen in the air is dissolved in the culture medium, the amount of dissolved oxygen is increased, and the conical flask is particularly suitable for culturing aerobic microorganisms.
Description
Technical field
The utility model belongs to the microorganism culturing field, is specifically related to a kind of oxygenation triangular flask that improves the low-shearing power of mikrobe dissolved oxygen efficiency.
Background technology
As shown in Figure 1, triangular flask is a kind of the simplest, modal container that is used for microbial cultivation process, is widely used in the cultivation and the expansion of microorganism strains.The size of the triangular flask of existing standard is generally: the relation of bottleneck internal diameter a and body bottom surface radius r is a=0.4r; The relation of body bottom surface radius r and triangular flask height H is r=0.27H, and the height h of bottleneck upper edge to bottleneck lower edge and the relation of triangular flask height H are h=0.25H.But in practical application, also find; Because mainly being the air inlets through bottleneck, the ventilation of triangular flask and dissolved oxygen amount obtain; And the restriction of the triangular flask size of this specification and structure causes it the insufficient problem of dissolved oxygen amount of deficiency of air and then initiation in microbial cultivation process, often to occur easily; Especially for the bigger aerobic bacterial strain of oxygen requirement and engineering strain are cultivated; The deficiency of dissolved oxygen amount causes microorganism strains growth bad even the situation of bacteriolyze occurs easily; The design and the structure of obviously existing triangular flask have been difficult to satisfy the cultivation requirement of whole mikrobes, need be according to the more rational triangular flask of the characteristics design of different strains, to satisfy the needs that microorganism strains is cultivated.Meanwhile; Another the parameter-shearing force that influences the microorganism strains culture effect also has bigger influence to the cultivation of bacterial strain; In order to guarantee effective surviving rate of aerobic bacterial strain; Must guarantee when having high dissolved oxygen amount, to have lower shearing force, to reduce the loss of bacterial classification in the culturing process, this just has higher requirement to the design of triangular flask.
The utility model content
For this reason, the utility model technical problem to be solved is that prior art intermediate cam bottle is difficult to satisfy the problem of high oxygen requirement strain culturing, and then improves a kind of oxygenation triangular flask with high dissolved oxygen amount and low-shearing power.
For solving the problems of the technologies described above; The described low-shearing power oxygenation of the utility model triangular flask; On the inwall of said triangular flask body and near the basal surface position place, form the catch parallel with the bottom surface; Said catch is along the circumferential equally distributed n of said body, and n=4,6 or 8, and is preferably 4.
Said catch gos deep into the length d of said body and the relation of said triangular flask body bottom surface radius r is d=0.5r.
The relation of the height e of the said body of said catch distance bottom surface and the height H of said triangular flask is e=0.1H.
Said catch is c=0.08H along the width c of said body circumferential direction and the relation between the said triangular flask height H.
The relation of the upper edge internal diameter m of the bottleneck of said triangular flask and said triangular flask bottleneck internal diameter a is m=1.25a.
The relation of the distance b of the said bottleneck of the distance upper edge, bottleneck upper edge of said triangular flask and the distance h of the said bottleneck of distance lower edge, said triangular flask bottleneck upper edge is b=0.06h.
Relation between the distance h of the said bottleneck of distance lower edge, said bottleneck upper edge and the height H of said triangular flask is h=0.25H.
Relation between said bottleneck internal diameter a and the said body bottom surface radius r is a=0.4r.
Relation between said body bottom surface radius r and the said triangular flask height H is r=0.30H.
The volume of said triangular flask is 200mL, 250mL, 300mL, 500mL, 1000mL or 2000mL.
The described technique scheme of the utility model is compared prior art and is had the following advantages; 1, on the said triangular flask inwall and the catch that is provided with near body basal surface position place substratum with the triangular flask moving process in; Can play the effect of secondary stirring, help to increase the contact probability and the contact area of substratum and sterile air, make airborne oxygen more be dissolved in the substratum; Increase dissolved oxygen amount, be particularly useful for the usefulness of the cultivation of aerobic microbiological; 2, preferred said baffle plate and said triangular flask bottom surface laterally arrange, and can effectively reduce in the whipping process bacterial classification loss that the shearing force to mikrobe causes; 3, said catch is set to can increase the dissolved oxygen amount in the microbial cultivation process uniformly and effectively along circumferentially equally distributed 4,6 or 8 of bodies, guarantees that simultaneously the shearing force to bacterial strain is less in the culturing process; 4, optimize relation between each dimensional parameters that said triangular flask is set; Make the size scale of said triangular flask meet fluid mechanics principle more; Effectively promote the dissolved oxygen amount in the culturing process; Particularly be applicable to that height is aerobic but cell walls approaches the not bacterial strain of fingerprinting stress, the for example cultivation of bacterial strains such as mould, actinomycetes, mycobacterium.
Description of drawings
For the content that makes the utility model is more clearly understood,, the utility model is done further detailed explanation, wherein below according to the specific embodiment of the utility model and combine accompanying drawing
Fig. 1 is the structural representation of prior art intermediate cam bottle;
Fig. 2 is the structural representation of the described triangular flask of the utility model.
Reference numeral is expressed as among the figure: the 1-catch.
Embodiment
As shown in Figure 2; The described high dissolved oxygen triangular flask of the utility model comprises integrated bottleneck, bottleneck and body part, and the vertical section of said finish portion is inverted trapezoidal; The vertical section of said neck portion is a rectangle, and the vertical section of said body part is trapezoidal for what just putting.On the inwall of said triangular flask body and near the basal surface position place of body, form 4 catch 1 that laterally arrange with said bottom surface, said catch 1 is the circumferential uniform distribution along said body.
Said catch 1 gos deep into the length d of said body and the relation of said triangular flask body bottom surface radius r is d=0.5r.The relation of the height e of the said body of said catch 1 distance bottom surface and the height H of said triangular flask is e=0.1H.Said catch 1 is c=0.08H along the width c of said body circumferential direction and the relation between the said triangular flask height H.The relation of the upper edge internal diameter m of the bottleneck of said triangular flask and said triangular flask bottleneck internal diameter a is m=1.25a.The relation of the distance b of the said bottleneck of the distance upper edge, bottleneck upper edge of said triangular flask and the distance h of the said bottleneck of distance lower edge, said triangular flask bottleneck upper edge is b=0.06h.Relation between the distance h of the said bottleneck of distance lower edge, said bottleneck upper edge and the height H of said triangular flask is h=0.25H.Relation between said bottleneck internal diameter a and the said body bottom surface radius r is a=0.4r.Relation between said body bottom surface radius r and the said triangular flask height H is r=0.30H.
As structure that can conversion, said catch 1 can also be along circumferentially equally distributed 6 or 8.
The volume of the described triangular flask of the utility model is applicable to 200mL, 250mL, 300mL, 500mL, 1000mL or 2000mL.
Obviously, the foregoing description only be for explanation clearly done for example, and be not qualification to embodiment.For the those of ordinary skill in affiliated field, on the basis of above-mentioned explanation, can also make other multi-form variation or change.Here need not also can't give exhaustive to all embodiments.And conspicuous variation of being extended out thus or change still are among the protection domain of the invention.
Claims (10)
1. low-shearing power oxygenation triangular flask; It is characterized in that: on the inwall of said triangular flask body and near the basal surface position place, form the catch parallel (1) with the bottom surface; Said catch (1) is along the circumferential equally distributed n of said body, and n=4,6 or 8.
2. low-shearing power oxygenation triangular flask according to claim 1 is characterized in that, said catch (1) gos deep into the length d of said body and the relation of said triangular flask body bottom surface radius r is d=0.5r.
3. low-shearing power oxygenation triangular flask according to claim 2 is characterized in that, the relation of the height e of the said body of said catch (1) distance bottom surface and the height H of said triangular flask is e=0.1H.
4. low-shearing power oxygenation triangular flask according to claim 3 is characterized in that, said catch (1) is c=0.08H along the width c of said body circumferential direction and the relation between the said triangular flask height H.
5. according to the arbitrary described low-shearing power oxygenation triangular flask of claim 1-4, it is characterized in that the relation of the upper edge internal diameter m of the bottleneck of said triangular flask and said triangular flask bottleneck internal diameter a is m=1.25a.
6. low-shearing power oxygenation triangular flask according to claim 5 is characterized in that, the relation of the distance b of the said bottleneck of the distance upper edge, bottleneck upper edge of said triangular flask and the distance h of the said bottleneck of distance lower edge, said triangular flask bottleneck upper edge is b=0.06h.
7. low-shearing power oxygenation triangular flask according to claim 6 is characterized in that, the relation between the distance h of the said bottleneck of distance lower edge, said bottleneck upper edge and the height H of said triangular flask is h=0.25H.
8. low-shearing power oxygenation triangular flask according to claim 7 is characterized in that, the relation between said bottleneck internal diameter a and the said body bottom surface radius r is a=0.4r.
9. low-shearing power oxygenation triangular flask according to claim 8 is characterized in that, the relation between said body bottom surface radius r and the said triangular flask height H is r=0.30H.
10. according to the arbitrary described low-shearing power oxygenation triangular flask of claim 1-4, it is characterized in that the volume of said triangular flask is 200mL, 250mL, 300mL, 500mL, 1000mL or 2000mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220167506 CN202576417U (en) | 2012-04-19 | 2012-04-19 | Low-shear force aeration conical flask |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220167506 CN202576417U (en) | 2012-04-19 | 2012-04-19 | Low-shear force aeration conical flask |
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| Publication Number | Publication Date |
|---|---|
| CN202576417U true CN202576417U (en) | 2012-12-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201220167506 Expired - Lifetime CN202576417U (en) | 2012-04-19 | 2012-04-19 | Low-shear force aeration conical flask |
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| CN (1) | CN202576417U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015160614A1 (en) * | 2014-04-17 | 2015-10-22 | Corning Incorporated | Vessel for growth of biological entities |
| CN113647293A (en) * | 2021-07-20 | 2021-11-16 | 云阳县康诺百草农业科技有限公司 | Cultivation method for preventing toadstool strains from forming mycelium pellets |
-
2012
- 2012-04-19 CN CN 201220167506 patent/CN202576417U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015160614A1 (en) * | 2014-04-17 | 2015-10-22 | Corning Incorporated | Vessel for growth of biological entities |
| US10870822B2 (en) | 2014-04-17 | 2020-12-22 | Corning Incorporated | Vessel for growth of biological entities |
| CN113647293A (en) * | 2021-07-20 | 2021-11-16 | 云阳县康诺百草农业科技有限公司 | Cultivation method for preventing toadstool strains from forming mycelium pellets |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20121205 |