CN202576087U - Bioreactor adopting magnetoelectric induction - Google Patents

Abstract

The utility model discloses a bioreactor for activating anaerobic ammonium oxidizing bacteria induced by magnetoelectricity, which comprises a reactor body and a bottom-up water inlet buffer zone of the reactor, an upflow reactor, a three-phase separation zone, and a peripheral water bath The insulation cover is characterized in that: the three-phase separation area includes a first-level three-phase separation area and a second-level three-phase separation area, and a magnetic field generation system and an electric field generation system are also installed in the reactor body, and a circulating mixing and stirring system is also installed at the bottom of the tank. system, the magnetic field generating system and the electric field generating system are connected to the control system. The reactor can be used to activate bacteria with reduced biological activity or on the verge of inactivation, reverse the inactivation process, restore the biological activity of bacterial cells, and achieve the purpose of rejuvenation.

Description

A kind of magnetoelectric induction bioreactor

technical field

The utility model belongs to the field of bioreactors, in particular to a bioreactor for rejuvenating bacteria by adopting a magnetoelectric induction method.

Background technique

Many bacteria, especially those bacteria that humans need to use some of their characteristics to provide certain services to humans, will run efficiently for a period of time, and a certain activity will decline or even lose. It is necessary to repeatedly introduce new bacteria to maintain this performance efficiently For example, when people study the biological denitrification activity of anammox denitrification bacteria, they will encounter such a situation: the denitrification activity of anammox bacteria will gradually inactivate as the denitrification time of bacteria increases. As a result, it is completely lost or even blackened and smelly. Therefore, timely rejuvenation of bacteria and maintaining the stability of its denitrification activity are the prerequisites for engineering applications, eliminating nitrogen pollution for humans, and ensuring the nitrogen cycle.

Anaerobic ammonium oxidation is an important nitrogen cycle stage in nature, and it is also a new field of wastewater biological denitrification research. It plays an irreplaceable role in the engineering utilization of eliminating nitrogen pollution in water bodies, and has broad and far-reaching application prospects. It is currently an international It is one of the biggest research hotspots in the Upper Nitrogen Removal Technology Basin. Although the research on using anammox to carry out engineering practice is very popular in the world, in our country, the research is mostly in the laboratory stage, and one of the main problems is that after a period of time, the anammox bacteria often The phenomenon of gradual loss of denitrification activity occurs. If certain measures are not taken in time, a large number of inactivated bacteria will die and disintegrate, and the bacteria will turn black and become smelly.

As a conventional exogenous DNA transfer method, the electroporation method has the advantages of simple operation, rapidity, and high transformation efficiency. The transfer of genetic material is realized by the method of electrotransformation, so that the bacteria that have lost a certain characteristic can regain the characteristic, and it can also accelerate the genetic variation of the bacteria. It is a common method for rejuvenating bacteria and obtaining bacteria with specific properties. There have been many successful applications of electroporation in the gene manipulation of actinomycetes, such as in Streptomyces lividans , S. parvulus , S. vinaceus , crack Streptomyces ( S. rimosus ) etc. have had successful reports.

Magneto-induced biological effects have always been a research hotspot in the field of magnetic effects. A large number of studies at home and abroad have shown that after a magnetic field acts on an organism, it can cause a series of biological reactions in the organism. Some experts believe that the magnetic field will affect the protein macromolecules and enzymes in the organism, enhance the enzyme activity in the organism, and increase the enzyme reaction speed; in addition, the magnetic field will also increase the permeability of the biological cell membrane, so that water and oxygen molecules and some can be magnetically affected. Activated metal ions etc. are easy to enter cells. Through the biological effect experiment of magnetized sewage treatment, it can be found that when the magnetic flux density is 0.37T, the activity of aerobic microorganisms increases by 17.3%, and the primary productivity of algae increases from 8.4-10g/ ^m3 per day in the control sample to 16.9g/ ^m3 per day ; Dehydrogenase decolorization test shows that the presence of a magnetic field is beneficial to the growth and metabolism of microorganisms, thereby promoting the adsorption and utilization of organic matter, and enhancing the ability of microorganisms to oxidize and degrade organic matter. Therefore, magneto-induced bioeffects and electric (field) induced bioeffects play an important role in rejuvenating bacteria.

Using an external high-voltage electric field and magnetic field, based on the stress response of the biodielectric to the compound magnetic field, the biological effect of the magnetoelectric effect is studied, and the "frequency action window" and "power (amplitude)" of a certain strain are determined and obtained in the experiment. "action window" and "action time window", to find the law of biological effects, activate or rejuvenate the strains that are gradually losing a certain activity, so that the strains can regain their activity, maintain a certain state of action that is beneficial to humans, and use their specific traits for humans Serving human production and life is an effective way to often encounter and solve problems in the use of microorganisms.

At present, there are domestic patents for "electrical biological treatment device for refractory organic wastewater" (publication number: CN1435379), and "magnetic biological reaction separation device for organic wastewater treatment" patent (publication number: CN201593005U), but there is no No bioreactor for magnetoelectric induction of bacteria. Studies have shown that both magnetic field treatment and electric field treatment, as long as the intensity is appropriate, can produce beneficial effects on organisms, especially for the rejuvenation process of some degenerated strains, which has a good promoting effect.

Utility model content

The technical problem to be solved by the utility model is to provide a magnetoelectric induction bioreactor capable of generating a specific magnetic field, which can stimulate and induce inactivated bacterial cells, causing bacteria in the inactivation process to A certain change occurs in the physiological and biochemical process, thereby reversing the inactivation process, so that the activity of the inactivated bacterial cells can be restored, and the purpose of rejuvenation can be achieved.

The technical scheme that realizes the utility model purpose is as follows:

The utility model is a bioreactor activated by magnetoelectric induction of anammox bacteria, which includes a reactor body and a bottom-up water inlet buffer zone of the reactor body, an upflow reaction chamber, a three-phase separation area, a magnetic field and an electric field generation system And the outer peripheral water bath insulation jacket is different from the prior art: the three-phase separation area includes a first-level three-phase separation area and a second-level three-phase separation area, and a magnetic field generation system and an electric field generation system are also arranged in the reactor body. The bottom of the pool is also provided with an internal circulation mixing and stirring system, and the magnetic field generating system and the electric field generating system are connected with the control system.

The separation device of the first-level three-phase separation zone in the three-phase separation zone is a movable type, and its lower part is nested in the inner wall of the upflow reaction zone, and two ears are extended from the upper part, which are inverted to the lower end of the second-level three-phase separation zone. The inner wall can be separated from the main body of the reactor when necessary; a total of 6 baffles are set inside the secondary three-phase separation area. The baffles are movable and fixed on the secondary three-phase separator with card slots. The number can be reduced or increased, and the partitions between the baffles are communicated with the guide tube.

The electric field generating system includes electrified carbon rods and polar plates installed in the lower part of the reaction zone facing each other, and a stable direct current is passed through during use to generate a stable radiating electric field. By changing the magnitude of the current, electric fields of different intensities can be generated; by changing the direction of the plates and carbon rods, electric fields in different directions can be generated. The electric field generation system is connected to the control system, and the external control system can freely control the strength of the electric field, and The time, sequence and method of electric field action.

The magnetic field generation system includes another opposite or up and down energized solenoid coil installed in the lower part of the reaction zone, and a stable direct current is fed in to generate a stable uniform magnetic field inside the coil. By changing the magnitude of the current, magnetic fields of different strengths can be generated; by changing the direction of the energized coil, magnetic fields in different directions can be generated. The magnetic field generation system is connected to the control system, and the external control system can freely control the strength of the magnetic field and the effect of the magnetic field. time, sequence, and manner.

The characteristic of the internal circulation stirring device at the bottom of the pool is that the internal suction type internal circulation stirring device can absorb and discharge the mud-water mixture by sinking at the bottom, including positioning suction and mobile suction and discharge, which can make the sediment at the bottom of the influent buffer zone The sludge and the upper sludge are continuously tumbling and mixed to ensure that the settled sludge can fully enter the magnetoelectric induction area and accept the magnetoelectric induction effect, and make it difficult for the oxygen-enriched water on the surface to mix into the lower layer.

Advantages and beneficial effects of the utility model

1) New technology: the combination of magnetoelectric induction technology realizes the cultivation and rejuvenation of bacteria, and achieves the purpose of activating and inactivating anammox bacteria. It is difficult to activate inactivated or inactivated anammox bacteria with a single electric induction technique or magnetic induction technique. This reactor provides a magnetoelectric inductive bioreactor that can generate a specific electric field and magnetic field, and through the combined activation technology of electric field and magnetic field, it can stimulate and induce the inactivated bacterial cells, causing bacteria that are in the inactivation process A certain change occurs in the physiological and biochemical process, thereby reversing the inactivation process, so that the activity of the inactivated bacterial cells can be restored, and the purpose of rejuvenation can be achieved.

2) Ensuring that the sludge subjected to magnetic and electric induction is fully and uniformly mixed: through the suction and discharge of the submerged internal suction circulation stirring device at the bottom of the pool, the sludge deposited at the bottom of the influent buffer zone and the upper sludge are continuously mixed. Tumble and mix to ensure that the sedimented sludge can fully enter the magnetoelectric induction area, accept the magnetoelectric induction effect, and minimize the mixing of oxygen.

3) Conducive to the study of various action parameters and reaction indicators: through the control and combination of reaction conditions or parameters such as electric field, magnetic field, and temperature control, it is beneficial to explore various parameters of activation and inactivation bacteria, such as the optimal voltage range and current effect. Range, frequency range, in order to obtain the "optimum action window" of each parameter, provide conditions for the reactor to operate in the optimum action window, and maximize the activation efficiency; and make it under the same conditions as temperature and influent water quality, It is possible to compare and study the difference in physiological and biochemical activities between magnetoelectric induced strains and non-induced cultured strains.

Description of drawings:

Fig. 1 is the structural representation of the reactor in the embodiment.

Detailed ways:

Below in conjunction with accompanying drawing, the utility model is further described:

See Fig. 1, the water inlet buffer zone 1 in the lower part of the reactor of the utility model is a four-sided pyramid, and the bottom is provided with a water inlet pipe 10, and the upflow reaction zone 2 is formed from the buffer zone 1, which is square, and then the three-phase separation The three-phase separation area is divided into the first-level three-phase separation area 3 and the second-level three-phase separation area 4. The upper part of the three-phase separation area is provided with a water outlet pipe 11, and the top is equipped with a sealing cover and an air outlet 6. Upflow reaction The lower part of the zone 2 is equipped with a magnetoelectric induction device, including the main part of generating the electric field, the electrified carbon rod 7-1 and the pole plate 7-2, the main part of generating the magnetic field, the electrified coil 8, and the internal circulation stirring device 9. Anammox bacteria exist in the upflow reaction zone 2 in the form of granular sludge. The outside of the main body of the reactor is provided with a water bath insulation jacket 5, whose length and width are 1.5-2 times that of the upflow reaction zone 2 and equal in height, which can be used to maintain the temperature of the reactor pool.

The aspect ratio of the pyramid water inlet buffer zone 1 is 1:1:1.5, the inclination angle of the lower slope is 65o-75o, the upflow reaction zone 2 is connected to the upper end of the water inlet buffer zone 1, and the three-phase separation zone is connected to the top. The lower end accounts for 2/3-3/5 of the height of the main body of the reactor. The first-level three-phase separation device 3 is built between the reaction zone 2 and the second-level three-phase separation zone 4. It is a symmetrical prism with both ends open and contracted in the middle. The overall aspect ratio is 1:1: ( 2-2.5). The secondary three-phase separation zone 4 is directly connected to the upflow reaction zone 2, the height is 1/3-1/2 of the height of the reaction zone 2, and the upper end is equipped with a movable sealing cover, and the sealing cover is provided with an air outlet 6 .

The three-phase separation zone device is divided into two parts: a movable first-stage three-phase separation device 3 and a fixed three-phase separation zone 4. The lower part of the movable type 3 is nested in the inner wall of the upflow reaction zone 2, and two Ear 3-1 is upside down on the inner wall of the lower end of the secondary three-phase separation zone, and can be separated from the main body of the reactor when necessary. The cross-sectional area of the constricted portion 3-2 is 1/3-2/5 of the opening area. The total volume ratio of the fixed three-phase separator 4 to the main body of the reactor is (0.3-0.4):1, the angle β between the expanding wall and the reference horizontal plane is 45°-70°, and baffles 4-2 are arranged inside for a total of 6 pieces, the baffles are all fixed by card slots, and can be disassembled when necessary. The included angle γ between them and the reference horizontal plane is 65o-85o. The top of the device is flat. The distance between the bottom of the baffle and the inner wall of the expanding wall is 3-7cm. The partitions between the baffles communicate with each other through a draft tube. The movable three-phase separator and the detachable baffle are mainly designed to facilitate the taking out and putting in of the magnetoelectric induction device when the magnetoelectric induction device is overhauled.

The magnetoelectric induction part is divided into the energized carbon rod 7-1 and the pole plate 7-2 for generating an electric field, and the energized solenoid coil 8 for generating a magnetic field. The plate material is high-purity graphite plate or conductive metal plate, and the coil material is insulated metal wire. The electrified carbon rod 7-1 is located at the center of the electrified coil 8, and the height of both is 1/3-2/5 of the upflow reaction zone 2. The diameter of the rod 7-1 is 1/5-1/6 of the outer diameter of the spiral coil 8, and the electrode plate 7-2 is attached to the outside of the reaction zone 2, the height is equal to the height of the carbon rod 7-1, and the width is equal to the outer wall of the reaction zone 2 , the thickness is 3-5mm. The carbon rod 7-1 cooperates with the pole plate 7-2 to generate an electric field, and the solenoid coil 8 generates a magnetic field.

The internal circulation stirring device 9 includes a special submerged systemic suction circulation stirring mixing device and a control system. The submerged systemic suction circulation stirring mixing device can perform suction and discharge at a specific flow rate under the control of the control system, and can also be displaced according to needs. , which is mainly used to make all the sludge in the reactor undergo internal circulation stirring and mixing, so that most of the sludge accepts the magnetic and electric induction, and minimizes the mixing of oxygen.

The utility model is made of plexiglass. When in use, waste water enters from the bottom water inlet pipe 10, enters the upflow reaction zone 2 through the water inlet buffer zone 1, and contacts and reacts with the anammox bacteria in the buffer zone 1 and the reaction zone 2. After that, it flows through the three-phase separation zone, and finally flows out from the water outlet pipe 11. The anammox bacteria are stirred by the internal circulation stirring device 9 in the buffer zone 1, tumble and mix with the sludge in the lower part of the reaction zone 2, and accept the action of the magnetoelectric induction device. The peripheral water bath part 5 is a water bath insulation cover for maintaining the temperature of the reactor pool, which can effectively control the sludge temperature environment.

The magnetoelectric induction reaction cell body can be used to activate the anammox bacteria with reduced activity or inactivation, and can intuitively study the activity state of the anammox bacteria under the simultaneous action of magnetic field, electric field, or magnetic field, which is different from the normal state. Compared with anammox bacteria, it is beneficial to explore various parameters of the reactor operation, such as the optimum voltage range, current range, and frequency range, so as to obtain the "optimum action window" of each parameter, and provide a basis for the reactor in The optimal action window operation provides conditions to maximize the activation efficiency; and makes it possible to compare and study the difference in physiological and biochemical activities between magnetoelectrically induced bacteria and non-induced cultured bacteria under the same conditions such as temperature and influent water quality.

After the reactor is started, by changing the three-dimensional direction of the energized carbon rod 7-1-polar plate 7-2, and the energized solenoid coil 8, the following induction methods can be provided according to the action mode and direction of the electric field and magnetic field:

1. Single electric field action, the radial action direction can be divided into up and down, front and back, left and right, and each direction has two implementation modes of forward and reverse current or magnetic field;

2. Independent magnetic field action, the radial action direction can be divided into up and down, front and back, left and right, and each direction has two implementation modes of forward and reverse current or magnetic field;

3. The electric field and the magnetic field act at the same time, radial direction: the electric field is the front-to-back direction, and the magnetic field is the front-to-back direction. At the same time, each direction has two implementation modes of forward and reverse current or magnetic field;

4. The electric field and the magnetic field act at the same time, radial direction: the electric field is the front and rear direction, the magnetic field is the left and right direction, and each direction has two implementation modes of forward and reverse current or magnetic field;

5. The electric field and the magnetic field act at the same time, radial direction: the electric field is the front and rear direction, the magnetic field is the up and down direction, and each direction has two implementation modes of forward and reverse current or magnetic field;

6. The electric field and the magnetic field act at the same time, radial direction: the electric field is the left and right directions, and the magnetic field is the front and rear directions. At the same time, each direction has two implementation modes of forward and reverse current or magnetic field;

7. The electric field and the magnetic field act at the same time, radial direction: the electric field is the left and right directions, and the magnetic field is the left and right directions, and each direction has two implementation modes of forward and reverse current or magnetic field;

8. The electric field and the magnetic field act at the same time, radial direction: the electric field is left and right, and the magnetic field is up and down. At the same time, each direction has two implementation modes of forward and reverse current or magnetic field;

9. The electric field and the magnetic field act at the same time, radial direction: the electric field is the up and down direction, the magnetic field is the front and rear direction, and each direction has two implementation modes of forward and reverse current or magnetic field;

10. The electric field and the magnetic field act at the same time, radial direction: the electric field is the up and down direction, the magnetic field is the left and right direction, and each direction has two implementation modes of forward and reverse current or magnetic field;

11. The electric field and the magnetic field act at the same time, radial direction: the electric field is up and down, and the magnetic field is up and down. At the same time, each direction has two implementations of forward and reverse current or magnetic field.

Claims (5)

1. a magnetoelectricity is induced bio-reactor; Comprise reactor body and reactor body water inlet buffer zone, flow lifting type reaction chamber, three-phase separation area, periphery water bath heat preservation cover from bottom to top; It is characterized in that: three-phase separation area comprises one-level three-phase separation area and secondary three-phase separation area; In reactor body, also be provided with magnetic field and produce system and electric field generation system, also be provided with the internal recycle mixing and stirring system at the bottom of the pond, magnetic field produces system and links to each other with system with electric field generation system.

2. bio-reactor according to claim 1; It is characterized in that: the tripping device of the one-level three-phase separation area of described three-phase separation area is an active type; Its underpart is nested in flow lifting type reaction zone inwall; Two ears are extended on top, and back-off can separate with reactor body when needing in the inwall of secondary three-phase separation area lower end; Totally 6 on secondary three-phase separation area set inside baffle plate, baffle plate is an active type, is fixed on the secondary triphase separator with draw-in groove, and the number of internal baffle can reduce or increase in case of necessity, and subregion communicates with thrust-augmenting nozzle between baffle plate.

3. bio-reactor according to claim 1 is characterized in that: described electric field generation system comprise be contained in the reaction zone bottom relatively to energising carbon-point and pole plate, feed galvanic current stream during use, produce stable radial pattern electric field; Through changing size of current, can produce the electric field of varying strength; Through changing pole plate and carbon-point direction, can produce the electric field of different directions, electric field generation system links to each other with system, external system can freely be controlled the power of strength of electric field, and the time of electric field action, in proper order, mode.

4. bio-reactor according to claim 1; It is characterized in that: described magnetic field generation system comprise be loaded on the reaction zone bottom another relatively to or the energising solenoid of upward and downward; Feed galvanic current stream during use, coil inside produces stable uniform magnetic field; Through changing size of current, the magnetic field that can produce varying strength; Through changing energising solenoid direction, the magnetic field that can produce different directions, magnetic field produce system and link to each other with system, the power that external system can free controlling magnetic field intensity, and the time of the action of a magnetic field, in proper order, mode.

5. bio-reactor according to claim 1; It is characterized in that: at the bottom of the described pond internal recycle whipping appts be through be submerged at the bottom of the pond internal-suction type internal recycle whipping appts to mud mixture suction; Comprise location suction and portable suction, can make to be deposited in the into mud and the continuous tumble mixed of top mud of water buffer zone bottom, guarantee that sedimentary mud can both fully get into magnetoelectricity and induce the district; Accept the inducing action of magnetoelectricity, make the heavy-oxygen-enriched water on top layer be difficult for sneaking into lower floor again.

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