CN112830586A - Method for rapidly enriching denitrifying sulfur oxide flora - Google Patents
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/163—Nitrates
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention discloses a method for rapidly enriching denitrifying sulfur oxide flora. The technology is carried out according to a two-stage magnetic strengthening mode: in the first stage, the cleaned fresh activated sludge is inoculated into a magnetic induction reactor, an enrichment substrate solution required by growth of denitrifying sulfur oxide flora is added for stirring, mixing and culturing, the magnetic field is controlled to be 5-70mT, stirring is stopped after the substrate is completely utilized, supernatant is discharged after standing and precipitating, and the process is repeated for 2-3 times; in the second stage, the magnetic field intensity is adjusted to be 5-20mT, the culture process is repeated for 2-5 times until the cultured flora realizes rapid denitrification and keeps stable denitrification performance. The method generates a magnetic biological effect through magnetic field induction, and stimulates the rapid growth and metabolic activity of denitrifying sulfur oxide flora in the mixed flora to realize the rapid enrichment of the denitrifying sulfur oxide flora.
Description
Technical Field
The invention belongs to the technical field of environmental biology, and particularly relates to a method for rapidly enriching denitrifying sulfur oxide flora and improving the denitrification capability of the denitrifying sulfur oxide flora.
Background
Excessive discharge of nitrogen pollutants in sewage easily causes eutrophication of surface water and harms human health, so that China sets strict discharge standards for nitrogen in sewage. The traditional heterotrophic denitrification deep denitrification method has high economic cost, large sludge yield and easy secondary pollution, while the autotrophic denitrification has the advantages of high efficiency, low energy consumption, economy, relative safety and the like, and is very suitable for being widely applied to deep denitrification.
The sulfur autotrophic denitrification is a process of reducing reduced sulfur as an electron donor and nitrite or nitrate as an electron acceptor into nitrogen. This process is mainly performed by denitrifying sulfur oxidizing flora. However, denitrifying sulfur oxide bacteria grow slowly, and the start of engineering and the culture of bacteria are limited. In the practice of domesticating and enriching denitrifying sulfur oxide flora, the removal rate of the oxidation state nitrogen generally reaches 60 percent or more than 80 percent and is stable, namely the process of successfully starting the reactor is considered to generally take 22 to 65 days.
The patent provides a method for rapidly enriching high-efficiency denitrifying sulfur oxide flora, thereby solving the engineering problems of long starting time and difficult flora culture in a sulfur autotrophic denitrification process.
Disclosure of Invention
The invention aims to quickly enrich the high-efficiency denitrifying sulfur oxide flora.
The invention provides a method for rapidly enriching denitrifying sulfur oxide flora, which is characterized by comprising the following steps: an exogenous magnetic field with specific strength is applied to the mixed flora, the proliferation rate and activity of the denitrifying sulfur oxide flora are enhanced under the action of the magnetic field, and other microorganisms are inhibited by the magnetic field, so that the rapid screening and enrichment of target strains are realized.
The invention provides a device for rapidly enriching denitrifying sulfur oxide flora, which comprises: the device comprises an enrichment reactor (1), a magnetic field detection gauss meter (2), a static magnetic field generating device (3), a stirrer (4) and a temperature control device (5).
The structure of a magnetic induction reaction device for realizing the rapid enrichment of denitrifying sulfur oxide flora by utilizing magnetic induction is as follows: the enrichment reactor (1) is arranged in a static magnetic field generating device (3), and a detection probe of a magnetic field detection gaussmeter (2) is arranged at the center of the enrichment reactor (1). A stirrer (4) and a temperature control device (5) are assembled on the enrichment reactor (1). The material of the enrichment reactor (1) is characterized by non-magnetic shielding material.
Based on the device, the technical method for rapidly enriching denitrifying sulfur oxide flora through magnetic induction mainly comprises the following steps:
(1) and (3) cleaning fresh aerobic activated sludge for 2-3 times by using tap water to remove organic matters and biological metabolism intermediate products in water. The cleaned activated sludge is placed in an enrichment reactor (1).
(2) And preparing an enriched substrate solution. The substrate-enriched solution is characterized in that NO is a main component3 -、S2O3 2-(or S)2-Or simple substance S) so that the molar ratio of the nitrogen to the sulfur elements is 0.62 to 1.24. Specifically, the concentration of the active carbon can be 2.55g/LNaNO3，6g/LNa2S2O3·5H2O, other nutrient element component is 2g/L NaHCO3，0.8g/L K2HPO4，0.3g/L KH2PO4，0.4g/L NH4Cl，0.021g/L MgCl2·6H2O, 2mL/L of trace elements, 0.5g/LEDTA of trace elements, 0.07g/LCaCl of trace elements2·2H2O、0.05g/LCoCl2·6H2O、0.05g/LMnCl2·4H2O、0.04g/LZnSO4·7H2O、0.03g/LCuSO4·5H2O、0.01g/L(NH4)6Mo7O24·4H2O、0.01g/LFeSO4·7H2Preparing O;
(3) injecting the enrichment substrate solution into the enrichment reactor (1) to ensure that the initial sludge concentration is 2.0-5.0 g/L.
(4) The stirrer (4) is started to completely mix the sludge and the solution. Starting a temperature control device, and keeping the temperature of the enrichment reactor (1) at 20-35 ℃. And starting the static magnetic field generating device (3), and adjusting the field intensity of the magnetic field to ensure that the indication range of the magnetic field detection gaussmeter (2) at the center of the enrichment reactor (1) is 50-70 mT.
(5) The nitrate nitrogen concentration of the enrichment reactor (1) is continuously monitored. When the nitrate nitrogen removal rate is more than 90%, the stirrer (4) is stopped. After settling for 30 minutes, the supernatant in the enrichment reactor (1) is discharged.
(6) Repeating the steps (3) - (5) for 2-3 times.
(7) Changing the magnetic field strength in the step (4). The field intensity of the magnetic field is adjusted to ensure that the indication range of the magnetic field detection gaussmeter (2) at the center of the enrichment reactor (1) is between 5 and 20 mT. Repeating the steps (3) - (5) for 2-5 times.
The main components of the culture solution in the step (2) are reduced-state sulfur salt and nitrate, and the cationic form of the culture solution is not limited under the condition of similar solubility. Other nutrient elements are added according to the optimal nutrient condition required by the growth of denitrifying sulfur oxidizing flora.
It will be understood by those skilled in the art that various changes and modifications in form and detail may be made therein without departing from the spirit and scope of the invention, and these are to be considered as falling within the scope of the invention.
The technical invention principle is as follows:
the magnetic induction technology applied by the invention is a method for promoting the growth and activity of microorganisms by applying a magnetic field with certain intensity to induce and generate a magnetic biological effect in the process of culturing the microorganisms. The enzymes with paramagnetic centers, anisotropic membrane structures, metal ions, electrons and the like in cells of the denitrifying sulfur oxidizing bacteria are all influenced by a magnetic field, and the paramagnetic particles, substances and structures respond to the magnetic field and change of biological life activities caused by the magnetic field, namely the magnetic biological effect induced by the magnetic field. The creation of positive or negative magnetobiological effects within a cell depends on the strength of the external magnetic field. The method adopts a two-stage magnetic strengthening mode, implements the inhibitory control of the non-sulfur autotrophic denitrifying bacteria in the early stage, and realizes the competitive advantage of the sulfur autotrophic denitrifying bacteria; and (3) implementing an activation magnetic field of the denitrifying sulfur oxide flora in the later stage to promote the rapid growth and the enhanced denitrification capability of the denitrifying sulfur oxide flora. The method realizes the rapid and efficient enrichment of the functional strains.
The invention has the beneficial effects that:
through the steps, the growth and the activity of the denitrifying sulfur oxide flora are effectively promoted under the induction action of the magnetic field, functional bacteria with high-efficiency denitrification performance can be enriched and obtained within 10-13 days, the performance is stable, and the starting time of the sulfur autotrophic denitrification reactor is greatly shortened. The method is simple, safe, efficient and economical, the magnetic generation equipment can be repeatedly utilized, and no secondary pollution exists, so that the method is an effective method for quickly enriching the efficient denitrifying sulfur oxide flora and is suitable for wide popularization.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings used in the embodiments or the description of the prior art will be briefly described below.
FIG. 1 is a schematic structural diagram of a magnetic induction denitrification sulfoxidation flora enrichment reactor device provided by an embodiment of the invention;
wherein, the marks in the figure are respectively: the device comprises an enrichment reactor (1), a magnetic field detection gauss meter (2), a static magnetic field generating device (3), a stirrer (4) and a temperature control device (5).
Fig. 2 is a technical route diagram according to the present invention.
Examples
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples, but the present invention is not limited to the following examples.
The implementation of this patent is illustrated below with specific examples.
In this example, the enrichment substrate solution used had a composition (g/L) of 2.55g/LNaNO3，6g/LNa2S2O3·5H2O，2g/LNaHCO3，0.8g/LK2HPO4，0.3g/LKH2PO4，0.4g/LNH4Cl，0.021g/LMgCl2·6H2O, 2mL/L trace elements (trace elements in terms of 0.5g/LEDTA, 0.07 g/LCaCl)2·2H2O、0.05g/LCoCl2·6H2O、0.05g/LMnCl2·4H2O、0.04g/LZnSO4·7H2O、0.03g/LCuSO4·5H2O、0.01g/L(NH4)6Mo7O24·4H2O、0.01g/LFeSO4·7H2O formulation).
(1) Constructing a magnetic induction reaction device consisting of an enrichment reactor, a static magnetic field generating device, a gauss meter, a stirrer and a temperature control device; the static magnetic field generating device comprises two permanent magnets with surface magnetic field intensity of 270mT and a set of magnet fixing frame; the used enrichment reactor is a known SBR reactor, and the effective volume is 3L; the temperature control device is a heating rod; the SBR reactor is placed in a static magnetic field generating device, a detection probe of a magnetic field detection gauss meter is placed in the center of the SBR reactor, and a stirrer and a heating rod are assembled on the SBR reactor.
(2) Fresh aerobic activated sludge taken from a sewage treatment plant is washed for three times by tap water and then is placed in an SBR reactor.
(3) Injecting the prepared enrichment substrate solution to 3L to ensure that the sludge concentration is 4 g/L.
(4) Adjusting the rotating speed of the stirrer to be 100rmp for mixing and stirring; starting a heating rod to control the temperature to be 30 ℃; adjusting the magnet fixing frame to enable the distance between the two magnets to be 15.5cm, adjusting the height of the magnets, enabling the bottom of each magnet to be 10cm away from the bottom plane of the reactor, and measuring the intensity of the central magnetic field in the reactor to be 50mT by using a gaussmeter;
(5) continuously monitoring the nitrate nitrogen concentration in the SBR reactor, and stopping the stirrer when the nitrate nitrogen removal rate is more than 90%; after standing and precipitating for 30 minutes, discharging the supernatant in the SBR reactor.
(6) Repeating the steps (3) to (5)2 times for 7 days.
(7) The magnet holder was adjusted so that the distance between the two magnets was 17cm and the magnetic field at the center of the SBR reactor was 5 mT. Repeating the steps (3) - (5)3 times for 6 days.
Through detection, the denitrification efficiency at the end of the enrichment cycle is 99.92-99.94%, the continuous three days are stable, and the enrichment cycle is 13 days. The enrichment period of the experimental group induced by applying the magnetic field is 12 days shorter than that of the blank group without applying the magnetic field, and about 1/2 starting time can be saved.
Claims (2)
1. An apparatus for rapidly enriching denitrifying sulfur oxide flora, comprising: the device comprises an enrichment reactor (1), a magnetic field detection gaussmeter (2), a static magnetic field generating device (3), a stirrer (4) and a temperature control device (5);
placing the enrichment reactor (1) in a static magnetic field generating device (3), and placing a detection probe of a magnetic field detection gaussmeter (2) in the center of the enrichment reactor (1); assembling a stirrer (4) and a temperature control device (5) on the enrichment reactor (1); the material of the enrichment reactor (1) is characterized by non-magnetic shielding material.
2. Method for applying the device according to claim 1, characterized by the steps of:
(1) cleaning aerobic activated sludge for 2-3 times by using tap water, and placing the cleaned activated sludge in an enrichment reactor (1);
(2) preparing an enrichment substrate solution; the enriched substrate solution was characterized as 2.55g/LNaNO3，6g/LNa2S2O3·5H2O, other nutrient element component is 2g/L NaHCO3，0.8g/L K2HPO4，0.3g/L KH2PO4，0.4g/L NH4Cl，0.021g/L MgCl2·6H2O, 2mL/L of trace elements and water as a solvent; the trace elements are calculated according to 0.5g/LEDTA and 0.07g/LCaCl2·2H2O、0.05g/LCoCl2·6H2O、0.05g/LMnCl2·4H2O、0.04g/LZnSO4·7H2O、0.03g/LCuSO4·5H2O、0.01g/L(NH4)6Mo7O24·4H2O、0.01g/LFeSO4·7H2Preparing O;
(3) injecting the enrichment substrate solution into an enrichment reactor (1) to ensure that the initial sludge concentration is 2.0-5.0 g/L;
(4) starting the stirrer (4) to completely mix the sludge and the enriched substrate solution; starting a temperature control device, and keeping the temperature of the enrichment reactor (1) at 20-35 ℃; starting the static magnetic field generating device (3), adjusting the field intensity of the magnetic field, and enabling the indication range of the magnetic field detection gaussmeter (2) at the center of the enrichment reactor (1) to be 50-70 mT;
(5) continuously monitoring the nitrate nitrogen concentration of the enrichment reactor (1); when the nitrate nitrogen removal rate is more than 90%, stopping the stirrer (4); after standing and settling for 30 minutes, discharging the supernatant in the enrichment reactor (1);
(6) repeating the steps (3) - (5) for 2-3 times;
(7) changing the magnetic field strength in the step (4); adjusting the field intensity of the magnetic field to ensure that the indication range of the magnetic field detection gaussmeter (2) at the center of the enrichment reactor (1) is between 5 and 20 mT; repeating the steps (3) - (5) for 2-5 times.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113797742A (en) * | 2021-10-12 | 2021-12-17 | 中国石油化工股份有限公司 | Quick starting and regulating method for biological desulfurization sludge system |
| CN115536144A (en) * | 2021-06-29 | 2022-12-30 | 中国石油化工股份有限公司 | Rapid enrichment method and device for sulfur autotrophic denitrifying bacteria |
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| CN104909468A (en) * | 2015-06-30 | 2015-09-16 | 南京大学 | Device and method for treating low-temperature high-ammonia nitrogen wastewater by use of constant magnetic field |
| CN105543282A (en) * | 2015-12-22 | 2016-05-04 | 湘潭大学 | A method of increasing an anaerobic biological hydrogen production yield from organic waste water or waste |
| CN110304712A (en) * | 2019-04-12 | 2019-10-08 | 温州创源环境科技有限公司 | Carry sulphur bamboo fibre magnetic suspension filler |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1401762A (en) * | 2002-09-06 | 2003-03-12 | 中南大学 | Method for screening ore leaching bacteria strain using dipyrite |
| CN104909468A (en) * | 2015-06-30 | 2015-09-16 | 南京大学 | Device and method for treating low-temperature high-ammonia nitrogen wastewater by use of constant magnetic field |
| CN105543282A (en) * | 2015-12-22 | 2016-05-04 | 湘潭大学 | A method of increasing an anaerobic biological hydrogen production yield from organic waste water or waste |
| CN110304712A (en) * | 2019-04-12 | 2019-10-08 | 温州创源环境科技有限公司 | Carry sulphur bamboo fibre magnetic suspension filler |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115536144A (en) * | 2021-06-29 | 2022-12-30 | 中国石油化工股份有限公司 | Rapid enrichment method and device for sulfur autotrophic denitrifying bacteria |
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