CN202519245U - Bio-enzyme purifying and separating device - Google Patents

Bio-enzyme purifying and separating device Download PDF

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Publication number
CN202519245U
CN202519245U CN 201120481145 CN201120481145U CN202519245U CN 202519245 U CN202519245 U CN 202519245U CN 201120481145 CN201120481145 CN 201120481145 CN 201120481145 U CN201120481145 U CN 201120481145U CN 202519245 U CN202519245 U CN 202519245U
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China
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valve
pump
trapped fluid
micro
filtrate
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Expired - Fee Related
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CN 201120481145
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Inventor
陈富林
宋志强
李毓枫
张志强
钟鸣
刘玉梅
岳建平
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XI'AN REJE BIOLOGICAL TECHNOLOGY Co Ltd
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XI'AN REJE BIOLOGICAL TECHNOLOGY Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10Separation or concentration of fermentation products
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12Purification

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

The utility model relates to a bio-enzyme purifying and separating device, which comprises a raw material storage tank, a Grundfos pump, a microfiltration pipe, a trapped fluid collecting pump, a washing tank, a flowrate adjusting device, a valve, a pressure gage, a flowmeter and a feed pipe, wherein the raw material storage tank is connected with the Grundfos pump through a feeding valve, and a return feed pipe is connected with a return valve through the flowmeter; the washing tank is connected with the Grundfos pump through the feed pipe and a water intake valve; and the microfiltration pipe is connected with the Grundfos pump through a reverse flushing valve and a flushing valve and is connected with a pump opening of the trapped fluid collecting pump through a trapped fluid recovery valve, a feeding control valve and the pressure gage. The bio-enzyme purifying and separating pump disclosed by the utility model can realize the purpose of continuously filtering and separating high-turbidity liquid under a completely enclosed state and keeps the high vitality of an enzymic preparation. According to the bio-enzyme purifying and separating device disclosed by the utility model, due to adoption of the microfiltration pipe which has the pore diameter of 0.1-0.2mum, high strength and resistance to acid and alkali and corrosion and is made of a hollow fiber organic film or ceramic or stainless steel assembly, efficient impurity removal and bacterial removal effects and separating and purifying functions can be achieved, original ingredients, structure, flavor and vitality of materials cannot be damaged, and the hygienic quality and character of products can be improved.

Description

A kind of enzyme purifies and separates device
Technical field
The utility model belongs to biological technical field, is specifically related to a kind of hydrolysis enzyme micro-filtration purifies and separates device.
Background technology
The hydrolysis enzyme needs separation and purification in leaching process, and the removal of impurities of traditional common filter, degerming ability are poor, and open operating process is prone to contaminating microorganisms, can not satisfy the free of contamination high quality requirement of product; Though and traditional membrane separation plant has removal of impurities preferably, degerming ability, is not suitable for the separation and purification of industrial biological zyme extract, very easily stop up, separating power is limited continuously, and the enzyme activity loss is big, and practical value is low.
Summary of the invention
To above-mentioned technical problem, the utility model provides a kind of enzyme purifies and separates device of easy, efficient, design more reasonable.
The technical scheme that the utility model adopted is:
A kind of enzyme purifies and separates device; Comprise: raw material storage tank (1), Ge Laifu pump (2), micro-filtrate (3), trapped fluid collecting pump (24), purge tank (7), flow regulation device (3), valve, tensimeter (4), under meter (6) and material pipe; Raw material storage tank (1) adopts the material pipe to connect through feed valve (9) dative Lay good fortune pump (2), and the material pipe that refluxes is connected with reverse flow valve (12) through under meter (6); Purge tank (7) leads to charge tube, water intaking valve (9) dative Lay good fortune pump (2) connects; Micro-filtrate (3) adopts the material pipe to connect through counterflush valve (13), flush valve (15) dative Lay good fortune pump (2); Collect pumping hole (24) through trapped fluid recovery valve (17), feed control valve (11), tensimeter (4) and trapped fluid and be connected, in addition through stopping valve (8), water intaking valve (10), filtered liq valve (20), filtration stopping valve (21), swing to flush valve (14) and be connected with reverse flow valve (12); Rinse water is connected with purge tank (7) through rinse water reverse flow valve (16); Trapped fluid reclaims and adopts the material pipe to be connected with micro-filtrate (5) with raw material storage tank (1) with trapped fluid collecting pump (24) through trapped fluid reverse flow valve (17), filtered liq valve (20).
Said valve comprises stopping valve (8); Feed valve (9); Water intaking valve (10); Feed control valve (11); Reverse flow valve (12); Counterflush valve (13); Swing to flush valve (14); Flush valve (15); Clear water reverse flow valve (16); Trapped fluid recovery valve (17); Exhaust-valve (18); Storage tank exhaust-valve (19); Filtered liq control valve port (20); Filter stopping valve (21) and first stopping valve (22); The Plastic valve of second stopping valve (23) or stainless valve or ceramic valve.
Described micro-filtrate (5) adopts a plurality of micro-filtrates to be in parallel installed with; Described micro-filtrate (5) is tubular fibre organic membrane or pottery or the stainless steel assembly of 0.1-0.2 μ m for the aperture.
Said flow regulation device (3) adopts the speed governing frequency transformer.
Said device is the close system of full envelope.
The utility model has following advantage with respect to prior art:
1) this device can be implemented in that continuous filtration separates high turbidity liquid under the complete closed state.Promptly when the micro-filtrate membrane filtration flux depression is arrived to a certain degree; Only need open the trapped fluid recovery valve, through the recovery channel pump trapped fluid in the micro-filtrate drawn back raw material storage tank lixiviate again, and use flushing with clean water; Thereby reach circulating filtration, make microfiltration membrane can keep the high-throughput long-play;
2) this device can keep the high vigor of zymin.Promptly regulate feed rate through flow regulation device control Ge Laifu pump; Through regulating reverse flow valve controlling diaphragm pressure; And jacketed type is incubated the temperature of controlling the head tank raw material; Reduce mechanical rotation, flow etc. acts on the destruction to enzyme, plays the effect that keeps the biological enzyme formulation vigor not lose;
3) this device employing aperture is the micro-filtrate of the high strength anti soda acid of 0.1-0.2 μ m, corrosion resistant tubular fibre organic membrane or pottery or stainless steel assembly; Can be when the efficient impurity removal degerming realizes the separation and purification effect; Can not destroy original composition, structure, local flavor, the vigor of material again; Improve the hygienic quality and the quality of product, thereby saved the numerous and diverse of traditional high-temperature sterilizing process, reduced the risk of high-temperature sterilization destruction material effective constituent; Not only but save energy but also can prevent that effective constituent is destroyed had prolonged the quality guaranteed period of product greatly.
Therefore, this device can be widely used in the filtering separation to the strict various biological enzyme formulations of mikrobe health and enzyme activity, also is applicable to the filtering separation of some simple liquid beverages and seasonings simultaneously.
Description of drawings
Fig. 1 is the structural representation of the utility model;
Embodiment
Further describe the utility model below in conjunction with accompanying drawing and through embodiment, but the utility model is not limited in this.
Visible by Fig. 1; A kind of enzyme purifies and separates device; Comprise: raw material storage tank (1), Ge Laifu pump (2), micro-filtrate (3), trapped fluid collecting pump (24), purge tank (7), flow regulation device (3), valve, tensimeter (4), under meter (6) and material pipe; Raw material storage tank (1) adopts the material pipe to connect through feed valve (9) dative Lay good fortune pump (2), and the material pipe that refluxes is connected with reverse flow valve (12) through under meter (6); Purge tank (7) leads to charge tube, water intaking valve (9) dative Lay good fortune pump (2) connects; Micro-filtrate (3) adopts the material pipe to connect through counterflush valve (13), flush valve (15) dative Lay good fortune pump (2); Be connected with trapped fluid collecting pump (24) through trapped fluid recovery valve (17), feed control valve (11), tensimeter (4), in addition through stopping valve (8), water intaking valve (10), filtered liq valve (20), filtration stopping valve (21), swing to flush valve (14) and be connected with reverse flow valve (12); Rinse water is connected with purge tank (7) through rinse water reverse flow valve (16); Trapped fluid reclaims and adopts the material pipe to be connected with micro-filtrate (5) with raw material storage tank (1) with trapped fluid collecting pump (24) through trapped fluid reverse flow valve (17), filtered liq valve (20).Micro-filtrate (5) adopts a plurality of micro-filtrates to be in parallel installed with; Micro-filtrate (5) is tubular fibre organic membrane or pottery or the stainless steel assembly of 0.1-0.2 μ m for the aperture.
The working process of the utility model device is:
Feed lines is: the material of raw material storage tank (1) is connected with the lower end opening for feed of micro-filtrate (5) through Ge Laifu pump (2), valve (9), (11) through plastics or stainless steel feed-pipe.Through the tensimeter of installing on the pipeline between valve (11) and the micro-filtrate (5) (4), observe the pressure condition of work system; Ge Laifu pump (2) is regulated flow through the flow regulation device (3) that is installed on the electrical control cubicles; The discharge port of micro-filtrate (5) upper end is delivered to concentrating unit through plastics or stainless steel feed-pipe through filtered liq valve mouth (20) and concentrates.The bypass outlet of the left and right sides is connected backflow through second valve (23), first valve (22), reverse flow valve (12) and under meter (6) with raw material storage tank (1) through plastics or stainless steel transfer lime in parallel.
The cleaning circuit is: purge tank (7) links to each other with micro-filtrate through Ge Laifu pump (2), valve (10), (11) through plastics or stainless steel feed-pipe, through the scavenging solution of micro-filtrate (5) through the upper end outlet of micro-filtrate (5) through plastics or stainless steel transfer lime through valve (21), (22), (16) to cleaning return tank;
The trapped fluid recovery train is: the trapped fluid in the raw material storage tank (1) through plastics or stainless steel transfer lime through Ge Laifu pump (2), trapped fluid collecting pump (2); Valve (9), (11), (17) are extracted into raw material storage tank (1) lixiviate again: the trapped fluid that remains in micro-filtrate (5) the inside through plastics or stainless steel transfer lime through recovery valve (17); Trapped fluid collecting pump (24) is extracted into raw material storage tank (1) lixiviate again.

Claims (5)

1. enzyme purifies and separates device; Comprise raw material storage tank (1), Ge Laifu pump (2), micro-filtrate (3), trapped fluid collecting pump (24), purge tank (7), flow regulation device (3), valve, tensimeter (4), under meter (6) and material pipe; It is characterized in that: raw material storage tank (1) adopts the material pipe to connect through feed valve (9) dative Lay good fortune pump (2), and the material pipe that refluxes is connected with reverse flow valve (12) through under meter (6); Purge tank (7) leads to charge tube, water intaking valve (9) dative Lay good fortune pump (2) connects; Micro-filtrate (3) adopts the material pipe to connect through counterflush valve (13), flush valve (15) dative Lay good fortune pump (2); Collect pumping hole (24) through trapped fluid recovery valve (17), feed control valve (11), tensimeter (4) and trapped fluid and be connected, in addition through stopping valve (8), water intaking valve (10), filtered liq valve (20), filtration stopping valve (21), swing to flush valve (14) and be connected with reverse flow valve (12); Rinse water is connected with purge tank (7) through rinse water reverse flow valve (16); Trapped fluid reclaims and adopts the material pipe to be connected with micro-filtrate (5) with raw material storage tank (1) with trapped fluid collecting pump (24) through trapped fluid reverse flow valve (17), filtered liq valve (20).
2. a kind of enzyme purifies and separates device according to claim 1 is characterized in that: said valve comprises stopping valve (8), feed valve (9), water intaking valve (10), feed control valve (11), reverse flow valve (12), counterflush valve (13), swings to flush valve (14), flush valve (15), clear water reverse flow valve (16), trapped fluid recovery valve (17), exhaust-valve (18), storage tank exhaust-valve (19), filtered liq control valve port (20), filter Plastic valve or the stainless valve or the ceramic valve of stopping valve (21) and first stopping valve (22), second stopping valve (23).
3. a kind of enzyme purifies and separates device according to claim 1 is characterized in that: described micro-filtrate (5) adopts a plurality of micro-filtrates to be in parallel installed with; Described micro-filtrate (5) is tubular fibre organic membrane or pottery or the stainless steel assembly of 0.1-0.2 μ m for the aperture.
4. a kind of enzyme purifies and separates device according to claim 1 is characterized in that: said flow regulation device (3) adopts the speed governing frequency transformer.
5. a kind of enzyme purifies and separates device according to claim 1 is characterized in that: said device is the close system of full envelope.
CN 201120481145 2011-11-28 2011-11-28 Bio-enzyme purifying and separating device Expired - Fee Related CN202519245U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201120481145 CN202519245U (en) 2011-11-28 2011-11-28 Bio-enzyme purifying and separating device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201120481145 CN202519245U (en) 2011-11-28 2011-11-28 Bio-enzyme purifying and separating device

Publications (1)

Publication Number Publication Date
CN202519245U true CN202519245U (en) 2012-11-07

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ID=47102205

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 201120481145 Expired - Fee Related CN202519245U (en) 2011-11-28 2011-11-28 Bio-enzyme purifying and separating device

Country Status (1)

Country Link
CN (1) CN202519245U (en)

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GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20121107

Termination date: 20151128