CN103131630A - Biological enzyme purification separating device - Google Patents
Biological enzyme purification separating device Download PDFInfo
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- CN103131630A CN103131630A CN 201110384481 CN201110384481A CN103131630A CN 103131630 A CN103131630 A CN 103131630A CN 201110384481 CN201110384481 CN 201110384481 CN 201110384481 A CN201110384481 A CN 201110384481A CN 103131630 A CN103131630 A CN 103131630A
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- trapped fluid
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Abstract
The invention provides a biological enzyme purification separating device. The biological enzyme purification separating device comprises a raw material storage tank, a Graef pump, a micro-filter tube, a rejection liquid collection pump, a wash tank, a flow adjusting device, a valve, a pressure gage, a flow gauge, and a material tube. The biological enzyme purification separating device can achieve continuous filtering and separating of high turbidity liquid under the fully sealed condition and maintaining of high activity of zymin. Due to the fact that the micro-filter tube, with the pore diameter of 0.1-0.2 microns and high-strength, acid-base resistance and corrosion resistance, of a hollow fiber organic membrane or ceramic or acstainless steel component is adopted, the biological enzyme purification separating device can efficiently conduct edulcoration and degerming and achieve separation and purification effect, at the same time, cannot damage an original ingredient, an original structure, an original special flavor, and original energy activity, improves sanitary quality of products. The biological enzyme purification separating device is widely applied for filtering and separation of variousbiological enzymes with strict demands for microorganismsanitation and enzyme activity and at the same time is further suitable for filtering and separation of certain simple liquid state drinks and condiments.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of hydrolysis biological enzyme micro-filtration purifies and separates device.
Background technology
The hydrolysis biological enzyme needs separation and purification in leaching process, and the removal of impurities of traditional common filter, degerming ability are poor, and the easy contaminating microorganisms of open operating process, can not satisfy the free of contamination high quality requirement of product; Though and traditional membrane separation plant has removal of impurities preferably, degerming ability, is not suitable for the separation and purification of industrial biological zyme extract, very easily stop up, continuous separate is from limited in one's ability, and the enzyme activity loss is large, and practical value is low.
Summary of the invention
For above-mentioned technical problem, the invention provides provides a kind of easy, efficient, more rational biological enzyme purifies and separates device of design.
The technical solution adopted in the present invention is:
A kind of biological enzyme purifies and separates device, comprise: raw material storage tank (1), Ge Laifu pump (2), micro-filtrate (3), trapped fluid collecting pump (24), purge tank (7), flow regulation device (3), valve, tensimeter (4), under meter (6) and material pipe, raw material storage tank (1) adopts the material pipe to connect by feed valve (9) dative Lay good fortune pump (2), and the material pipe that refluxes is connected with reverse flow valve (12) by under meter (6); Purge tank (7) leads to charge tube, water intaking valve (9) dative Lay good fortune pump (2) connects; Micro-filtrate (3) adopts the material pipe to connect by counterflush valve (13), flush valve (15) dative Lay good fortune pump (2), collect pumping hole (24) by trapped fluid recovery valve (17), feed control valve (11), tensimeter (4) and trapped fluid and be connected, separately by stopping valve (8), water intaking valve (10), filtered liquid control valve (20), filtration stopping valve (21), swing to flush valve (14) and be connected 12 with reverse flow valve) connection; Rinse water is connected with purge tank (7) by rinse water reverse flow valve (16); Trapped fluid reclaims and adopts the material pipe to be connected 24 by trapped fluid reverse flow valve (17), filtered liquid control valve (20) with the trapped fluid collecting pump) be connected 5 with raw material storage tank (21) with micro-filtrate) be connected.
described valve comprises stopping valve (8), feed valve (9), water intaking valve (10), feed control valve (11), reverse flow valve (12), counterflush valve (13), swing to flush valve (14), flush valve (15), clear water reverse flow valve (16), trapped fluid recovery valve (17), exhaust-valve (18), storage tank exhaust-valve (19), filtered liquid is controlled valve port (20), filter Plastic valve or stainless valve or the ceramic valve of stopping valve (21) and stopping valve (22) (23).
Described micro-filtrate (5) adopts a plurality of micro-filtrates to be installed in parallel; Described micro-filtrate (5) is tubular fibre organic membrane or pottery or the stainless steel assembly of 0.1-0.2 μ m for the aperture.
Described flow regulation device (3) adopts speed regulation frequency converter.
Described device is the system that entirely is sealed.
The present invention has advantages of as follows with respect to prior art:
1) this device can realize that continuous filtration separates high turbidity liquid under complete closed state.Namely when the micro-filtrate membrane filtration flux depression to a certain extent the time, only need to open the trapped fluid recovery valve, by the recovery channel pump, the trapped fluid in micro-filtrate be drawn back raw material storage tank lixiviate again, and use flushing with clean water, thereby reach circulating filtration, make microfiltration membrane can keep the high-throughput long-play;
2) this device can keep the high vigor of zymin.Namely control the Ge Laifu pump by flow regulation device and regulate feed rate, by regulating reverse flow valve controlling diaphragm pressure, and jacketed heat insulation is controlled the temperature of head tank raw material, reduce mechanical rotation, flow etc. acts on the destruction to biological enzyme, plays the effect that keeps the biological enzyme formulation vigor not lose;
3) this device employing aperture is the micro-filtrate of the high strength acid resistant alkali of 0.1-0.2 μ m, corrosion resistant tubular fibre organic membrane or pottery or stainless steel assembly, can be when the efficient impurity removal degerming realizes the separation and purification effect, can not destroy again original composition, structure, local flavor, the vigor of material, hygienic quality and the quality of product have been improved, thereby saved the numerous and diverse of traditional high-temperature sterilizing process, reduced the risk of high-temperature sterilization destruction material effective constituent, not only but save energy but also can prevent that effective constituent is destroyed, extended the quality guaranteed period of product greatly.
Therefore, this device can be widely used in the filtering separation to microorganism health and the strict various biological enzyme formulations of enzyme activity, also is applicable to the filtering separation of some simple liquid beverages and seasonings simultaneously.
Description of drawings
Fig. 1 is structural representation of the present invention;
Embodiment
Further describe the present invention below in conjunction with accompanying drawing and by embodiment, but the present invention is not limited to this.
As seen from Figure 1, a kind of biological enzyme purifies and separates device, comprise: raw material storage tank (1), Ge Laifu pump (2), micro-filtrate (3), trapped fluid collecting pump (24), purge tank (7), flow regulation device (3), valve, tensimeter (4), under meter (6) and material pipe, raw material storage tank (1) adopts the material pipe to connect by feed valve (9) dative Lay good fortune pump (2), and the material pipe that refluxes is connected with reverse flow valve (12) by under meter (6); Purge tank (7) leads to charge tube, water intaking valve (9) dative Lay good fortune pump (2) connects; Micro-filtrate (3) adopts the material pipe to connect by counterflush valve (13), flush valve (15) dative Lay good fortune pump (2), be connected with trapped fluid collecting pump (24) by trapped fluid recovery valve (17), feed control valve (11), tensimeter (4), separately by stopping valve (8), water intaking valve (10), filtered liquid control valve (20), filter stopping valve (21), swing to flush valve (14) and be connected 12 with reverse flow valve) connection; Rinse water is connected with purge tank (7) by rinse water reverse flow valve (16); Trapped fluid reclaims and adopts the material pipe to be connected 24 by trapped fluid reverse flow valve (17), filtered liquid control valve (20) with the trapped fluid collecting pump) be connected 5 with raw material storage tank (21) with micro-filtrate) be connected.Micro-filtrate (5) adopts a plurality of micro-filtrates to be installed in parallel; Micro-filtrate (5) is tubular fibre organic membrane or pottery or the stainless steel assembly of 0.1-0.2 μ m for the aperture.
The working process of apparatus of the present invention is:
Feed lines is: the material of raw material storage tank (1) is connected with the lower end opening for feed of micro-filtrate (5) through Ge Laifu pump (2), valve (9), (11) by plastics or stainless steel feed-pipe.By the tensimeter (4) of installing on the pipeline between valve (11) and micro-filtrate (5), observe the pressure condition of work system; Ge Laifu pump (2) carries out adjust flux by the flow regulation device (3) that is arranged on electrical control cubicles; The discharge port of micro-filtrate (5) upper end by plastics or stainless steel feed-pipe after filtration hydraulic control valve mouth (20) be delivered to concentrating unit and concentrate.The bypass outlet of the left and right sides is connected backflow through valve port (23), (22), (12) and under meter (6) with raw material storage tank (1) by plastics or stainless steel transfer lime in parallel.
The cleaning circuit is: purge tank (7) is connected with micro-filtrate through Ge Laifu pump (2), valve (10), (11) by plastics or stainless steel feed-pipe, through the scavenging solution of micro-filtrate (5) through the upper end outlet of micro-filtrate (5) by plastics or stainless steel transfer lime through valve (21), (22), (16) to cleaning return tank;
The trapped fluid recovery train is: the trapped fluid in raw material storage tank (1) by plastics or stainless steel transfer lime through Ge Laifu pump (2), trapped fluid collecting pump (2), valve (9), (11), (17) are extracted into raw material storage tank (1) lixiviate again: the trapped fluid that remains in micro-filtrate (5) the inside by plastics or stainless steel transfer lime through recovery valve (17), trapped fluid collecting pump (24) is extracted into raw material storage tank (1) lixiviate again.
Claims (5)
1. biological enzyme purifies and separates device, comprise raw material storage tank (1), Ge Laifu pump (2), micro-filtrate (3), trapped fluid collecting pump (24), purge tank (7), flow regulation device (3), valve, tensimeter (4), under meter (6) and material pipe, it is characterized in that: raw material storage tank (1) adopts the material pipe to connect by feed valve (9) dative Lay good fortune pump (2), and the material pipe that refluxes is connected with reverse flow valve (12) by under meter (6); Purge tank (7) leads to charge tube, water intaking valve (9) dative Lay good fortune pump (2) connects; Micro-filtrate (3) adopts the material pipe to connect by counterflush valve (13), flush valve (15) dative Lay good fortune pump (2), collect pumping hole (24) by trapped fluid recovery valve (17), feed control valve (11), tensimeter (4) and trapped fluid and be connected, separately by stopping valve (8), water intaking valve (10), filtered liquid control valve (20), filtration stopping valve (21), swing to flush valve (14) and be connected 12 with reverse flow valve) connection; Rinse water is connected with purge tank (7) by rinse water reverse flow valve (16); Trapped fluid reclaims and adopts the material pipe to be connected 24 by trapped fluid reverse flow valve (17), filtered liquid control valve (20) with the trapped fluid collecting pump) be connected 5 with raw material storage tank (21) with micro-filtrate) be connected.
2. a kind of biological enzyme purifies and separates device according to claim 1, it is characterized in that: described valve comprises stopping valve (8), feed valve (9), water intaking valve (10), feed control valve (11), reverse flow valve (12), counterflush valve (13), swing to flush valve (14), flush valve (15), clear water reverse flow valve (16), trapped fluid recovery valve (17), exhaust-valve (18), storage tank exhaust-valve (19), filtered liquid is controlled valve port (20), filter Plastic valve or stainless valve or the ceramic valve of stopping valve (21) and stopping valve (22) (23).
3. a kind of biological enzyme purifies and separates device according to claim 1 is characterized in that: described micro-filtrate (5) adopts a plurality of micro-filtrates to be installed in parallel; Described micro-filtrate (5) is tubular fibre organic membrane or pottery or the stainless steel assembly of 0.1-0.2 μ m for the aperture.
4. a kind of biological enzyme purifies and separates device according to claim 1, is characterized in that: described flow regulation device (3) employing speed regulation frequency converter.
5. a kind of biological enzyme purifies and separates device according to claim 1, it is characterized in that: described device is the system that entirely is sealed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110384481 CN103131630A (en) | 2011-11-28 | 2011-11-28 | Biological enzyme purification separating device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110384481 CN103131630A (en) | 2011-11-28 | 2011-11-28 | Biological enzyme purification separating device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103131630A true CN103131630A (en) | 2013-06-05 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110384481 Withdrawn CN103131630A (en) | 2011-11-28 | 2011-11-28 | Biological enzyme purification separating device |
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| Country | Link |
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| CN (1) | CN103131630A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108325762A (en) * | 2018-03-13 | 2018-07-27 | 绍兴市上虞区中泰医疗科技有限公司 | A kind of biological respinse and bio-separation device |
| CN109593733A (en) * | 2018-12-31 | 2019-04-09 | 大连理工常熟研究院有限公司 | A kind of biological enzyme filtering technique using gas-powered |
| CN109651490A (en) * | 2018-12-18 | 2019-04-19 | 青海省畜牧兽医科学院 | A kind of D type clostridium botulinum toxin protein purification device and its method of purification |
| CN111304067A (en) * | 2020-03-10 | 2020-06-19 | 北京好思康科技有限公司 | Intermittent cleaning and filtering system |
| WO2021077456A1 (en) * | 2019-10-24 | 2021-04-29 | 中山大学 | Filter device and method used for microbial enzymatic activity testing |
| CN106591117B (en) * | 2016-12-30 | 2023-07-28 | 天津市诺奥科技发展有限公司 | Enzyme purification and separation equipment |
-
2011
- 2011-11-28 CN CN 201110384481 patent/CN103131630A/en not_active Withdrawn
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106591117B (en) * | 2016-12-30 | 2023-07-28 | 天津市诺奥科技发展有限公司 | Enzyme purification and separation equipment |
| CN108325762A (en) * | 2018-03-13 | 2018-07-27 | 绍兴市上虞区中泰医疗科技有限公司 | A kind of biological respinse and bio-separation device |
| CN109651490A (en) * | 2018-12-18 | 2019-04-19 | 青海省畜牧兽医科学院 | A kind of D type clostridium botulinum toxin protein purification device and its method of purification |
| CN109593733A (en) * | 2018-12-31 | 2019-04-09 | 大连理工常熟研究院有限公司 | A kind of biological enzyme filtering technique using gas-powered |
| WO2021077456A1 (en) * | 2019-10-24 | 2021-04-29 | 中山大学 | Filter device and method used for microbial enzymatic activity testing |
| CN111304067A (en) * | 2020-03-10 | 2020-06-19 | 北京好思康科技有限公司 | Intermittent cleaning and filtering system |
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Application publication date: 20130605 |