CN202440504U - Disposable cell passaging, blowing-beating and split packaging sucker - Google Patents

Disposable cell passaging, blowing-beating and split packaging sucker Download PDF

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Publication number
CN202440504U
CN202440504U CN 201220073419 CN201220073419U CN202440504U CN 202440504 U CN202440504 U CN 202440504U CN 201220073419 CN201220073419 CN 201220073419 CN 201220073419 U CN201220073419 U CN 201220073419U CN 202440504 U CN202440504 U CN 202440504U
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CN
China
Prior art keywords
sucker
suction pipe
bottle
main body
beating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN 201220073419
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Chinese (zh)
Inventor
杜娟
孙青�
曹莉莉
岳龙涛
孙宗国
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shandong Qianfoshan Hospital
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Shandong Qianfoshan Hospital
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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Priority to CN 201220073419 priority Critical patent/CN202440504U/en
Application granted granted Critical
Publication of CN202440504U publication Critical patent/CN202440504U/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/28Constructional details, e.g. recesses, hinges disposable or single use

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • External Artificial Organs (AREA)

Abstract

The utility model discloses a disposable cell passaging, blowing-beating and split packaging sucker. The sucker comprises a sucker body, wherein a spherical bag is arranged at the back end of the sucker body; a suction nozzle is arranged at the front end of the sucker body; and the sucker body is provided with a bended connecting part. The disposable cell passaging, blowing-beating and split packaging sucker is folded to have a certain angle according to a need to enable the suction nozzle to be dead against the direction of a bottle bottom, so that the cells at the bottom of the bottle can be blown and beaten conveniently and a single-cell suspension liquid is easier to form. In addition, as the suction nozzle is dead against the direction of the bottle bottom, the degree of pressing force of fingers can be lightened, foams generated by the liquid which impacts the bottle bottom violently are reduced and thus the damage from the foams to the cells is also reduced; the cells at two upper shoulder parts of the culture bottle can be blown down, so that the disadvantage that a direct sucker cannot blow the two upper shoulder parts of the culture bottle is avoided; the angle can be adjusted randomly and the sucker can be lightly touched and strengthened before suction of the cell suspension liquid and split packaging, so that a dropper can come in or go out of the bottle mouth conveniently to transfer and split-package the cell suspension liquid; and objects stuck on the inner wall of each position of the culture bottle can be sucked and scraped.

Description

Disposable passage, piping and druming, packing suction pipe
Technical field
The utility model relates to a kind of disposable passage, piping and druming, packing suction pipe.
Background technology
At clinical treatment and biological scientific research field, cell cultures is indispensable.,, also cell quantity is enlarged simultaneously because cell proliferation to a certain degree, comes in contact inhibition when cell, need from a culturing bottle, divide to install to cultivation in the different bottles in order to make the continued growth of cell ability.At present often use suction pipe to carry out passage, piping and druming, packing, this suction visitor comprises suction pipe main body, suction nozzle and microscler sacculus portion.During use, push microscler sacculus portion, draw the liquid in the culturing bottle, blow and beat facing to the cell of culturing bottle bottom and go down to posterity, and shift packing to a cell suspension of blowing out with hand.
The tip that is applied to the suction pipe of passage and packing at present is straight, in the time of the cell of piping and druming in the culturing bottle, is parallel operation with the bottom surface of culturing bottle.Cause the cell one that is in culturing bottle bottom to blow like this and a sheet ofly all fallen down, can not become single cell suspension.In addition, shoulder place on two of culturing bottle, straight tip can not blow to, and causes the cell at two shoulder places to go down to posterity, thereby can influence going down to posterity of whole bottle cell.If piping and druming time at the bottom of the body of suction pipe and the parallel plane words of piping and druming, liquid can impact bottle, is easy to generate foam, bubble has broken back moment and has produced huge impact power for cell, thereby influences the growth of cell.
Summary of the invention
The purpose of the utility model is for overcoming the deficiency of above-mentioned prior art, a kind of disposable passage, piping and druming, packing suction pipe being provided.
For realizing above-mentioned purpose, the utility model adopts following technical proposals:
A kind of disposable passage, piping and druming, packing suction pipe, it comprises the suction pipe main body, and the rear end of suction pipe main body is that sacculus, front end are suction nozzle, and the suction pipe main body is provided with crooked connection section.
Said suction pipe main body adopts plastics to process.
The connection section of said bending is made up of the ring-type fluctuating gauffer that is in folded state that several link to each other.
Bending angle between said suction pipe front end and the suction pipe rear end is 45-60 °.
Said suction pipe main body is one-body molded by plastic material.
Method of use: hand-held microscler sacculus portion puts in suction pipe in the bottle, above the shoulder place; Through the fold buffer zone, as required, folding has a certain degree with main body, suction nozzle; Draw the nutrient solution in the culturing bottle, blow and beat facing to the cell of culturing bottle bottom and go down to posterity, and before drawing cell suspension and packing; It is straight to flip folding, is convenient to pass in and out bottleneck, and carries out cell suspension and shift packing.
The beneficial effect of the utility model is, (1) is converted into certain angle (recommending the 45-60 degree) as required, make suction nozzle face toward bottle at the bottom of direction, be convenient to piping and druming to the bottle floor cells, more be prone to become single cell suspension; In addition,, can alleviate the dynamics of finger presses, reduce the foam that produces at the bottom of liquid fiercely impacts bottle, thereby also reduce the damage of foam pair cell owing to be direction at the bottom of suction nozzle faces toward bottle; (2) be converted into certain angle as required, can blow down the cell of two last shoulders of culturing bottle come, avoided direct suction can't blow to two shortcomings that go up shoulder of culturing bottle; (3) any adjustment of angle, before absorption cell suspension and the packing, it is straight to flip folding, is convenient to dropper turnover bottleneck, and carries out cell suspension and shift packing; (4) rotation of arbitrarily angled (180 degree in) can arrive each position of culturing bottle, inhales and scrapes off being bonded at object on the culturing bottle inwall.
Description of drawings
Fig. 1 is the structural representation of embodiment 1;
Fig. 2 is the structural representation of embodiment 2.
1. suction pipe main bodys wherein, 2. sacculus, 3. suction nozzle, 4. crooked connection section, 5. gauffer, α. bending angle.
Embodiment
Below in conjunction with accompanying drawing and embodiment the utility model is further specified.
Embodiment 1
As shown in Figure 1, a kind of disposable passage, piping and druming, packing suction pipe, it comprises suction pipe main body 1, and the rear end of suction pipe main body 1 is that sacculus 2, front end are suction nozzle 3, and suction pipe main body 1 is provided with crooked connection section 4.Bending angle α between said suction pipe front end and the suction pipe rear end (angle of the two medullary ray extended line) is 45 °.Said suction pipe main body 1 is one-body molded by plastic material.
Embodiment 2
As shown in Figure 2, as shown in Figure 1, a kind of disposable passage, piping and druming, packing suction pipe, it comprises suction pipe main body 1, suction pipe main body 1 is one-body molded by plastic material.The rear end of suction pipe main body 1 is that sacculus 2, front end are suction nozzle 3, and suction nozzle 3 parts also are straight-run of pipe, and suction pipe main body 1 is provided with crooked connection section 4, and crooked connection section 4 is made up of the ring-type fluctuating gauffer 5 that is in folded state that several link to each other.Bending angle α between suction pipe front end and the suction pipe rear end is between 0-180 °.

Claims (5)

1. a disposable passage, piping and druming, packing suction pipe, it comprises the suction pipe main body, and the rear end of suction pipe main body is that sacculus, front end are suction nozzle, it is characterized in that, and the suction pipe main body is provided with crooked connection section.
2. disposable passage as claimed in claim 1, piping and druming, packing suction pipe is characterized in that, said suction pipe main body adopts plastics to process.
3. disposable passage as claimed in claim 2, piping and druming, packing suction pipe is characterized in that, the connection section of said bending is made up of the ring-type fluctuating gauffer that is in folded state that several link to each other.
4. like each described disposable passage of claim 1-3, piping and druming, packing suction pipe, it is characterized in that the bending angle between said suction pipe front end and the suction pipe rear end is 45-60 °.
5. like each described disposable passage of claim 1-3, piping and druming, packing suction pipe, it is characterized in that said suction pipe main body is one-body molded by plastic material.
CN 201220073419 2012-03-01 2012-03-01 Disposable cell passaging, blowing-beating and split packaging sucker Expired - Fee Related CN202440504U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201220073419 CN202440504U (en) 2012-03-01 2012-03-01 Disposable cell passaging, blowing-beating and split packaging sucker

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201220073419 CN202440504U (en) 2012-03-01 2012-03-01 Disposable cell passaging, blowing-beating and split packaging sucker

Publications (1)

Publication Number Publication Date
CN202440504U true CN202440504U (en) 2012-09-19

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CN 201220073419 Expired - Fee Related CN202440504U (en) 2012-03-01 2012-03-01 Disposable cell passaging, blowing-beating and split packaging sucker

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CN (1) CN202440504U (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102586091A (en) * 2012-03-01 2012-07-18 山东省千佛山医院 Disposable cell passage, blowing, beating and subpackaging suction tube
WO2017054788A1 (en) * 2015-10-02 2017-04-06 Biovendor Instruments A.S. Multi-axis handling device designed to transfer biological samples and a method of transferring biological samples using the multi-axis handling device
TWI631337B (en) * 2012-10-29 2018-08-01 榮研化學股份有限公司 Sample inspection device
CN109370877A (en) * 2018-12-18 2019-02-22 章毅 Cell suspension subpackage apparatus
CN109628294A (en) * 2019-01-31 2019-04-16 济南市疾病预防控制中心 A kind of instant culture apparatus for the detection of flora most probable number
CN109679835A (en) * 2019-01-31 2019-04-26 济南市疾病预防控制中心 A kind of instant culture apparatus for most probable number method detection flora
CN109694817A (en) * 2019-01-31 2019-04-30 济南市疾病预防控制中心 A kind of culture apparatus of bacteria detection live suitable for public health emergency

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102586091A (en) * 2012-03-01 2012-07-18 山东省千佛山医院 Disposable cell passage, blowing, beating and subpackaging suction tube
TWI631337B (en) * 2012-10-29 2018-08-01 榮研化學股份有限公司 Sample inspection device
WO2017054788A1 (en) * 2015-10-02 2017-04-06 Biovendor Instruments A.S. Multi-axis handling device designed to transfer biological samples and a method of transferring biological samples using the multi-axis handling device
CN109370877A (en) * 2018-12-18 2019-02-22 章毅 Cell suspension subpackage apparatus
CN109628294A (en) * 2019-01-31 2019-04-16 济南市疾病预防控制中心 A kind of instant culture apparatus for the detection of flora most probable number
CN109679835A (en) * 2019-01-31 2019-04-26 济南市疾病预防控制中心 A kind of instant culture apparatus for most probable number method detection flora
CN109694817A (en) * 2019-01-31 2019-04-30 济南市疾病预防控制中心 A kind of culture apparatus of bacteria detection live suitable for public health emergency

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C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20120919

Termination date: 20130301