CN202440493U - Novel cell passage device - Google Patents
Novel cell passage device Download PDFInfo
- Publication number
- CN202440493U CN202440493U CN 201220073422 CN201220073422U CN202440493U CN 202440493 U CN202440493 U CN 202440493U CN 201220073422 CN201220073422 CN 201220073422 CN 201220073422 U CN201220073422 U CN 201220073422U CN 202440493 U CN202440493 U CN 202440493U
- Authority
- CN
- China
- Prior art keywords
- bottle
- suction nozzle
- main body
- dropper
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Devices For Use In Laboratory Experiments (AREA)
Abstract
The utility model discloses a novel cell passage device which comprises a rubber suction bulb and a dropper main body, wherein the back end of the dropper main body is connected with the rubber suction bulb, and the front end of the dropper main body is provided with a suction nozzle which is of a crooked shape. according to the design, the novel cell passage device is provided with a crooked suction nozzle with a 45-degree bended angle and had the following advantage: (1) the suction nozzle faces the bottom of a bottle, so as to conveniently flow and beat cells at the bottom of the bottle, thus the cells at the bottom of the bottle can easily become single cell suspension; in addition, as the suction nozzle faces the bottom of the bottle, the pressing strength of fingers can be reduced and foams generated when liquid impacts the bottom of the bottle seriously can be reduced, thereby reducing damage of foams to the cells; (2) cells on the upper shoulder parts of the culture bottle can be blown down, thereby overcoming the defect that a direct dropper can not blow the two upper shoulder parts of the culture bottle; and (3) objects adhered to the inner wall of the culture bottle can be sucked and scraped down easily.
Description
Technical field
The utility model relates to the passage technical field, is specifically related to a kind of novel device that is used for passage.
Background technology
The dropper that traditional passage is used, the long suction nozzle of its sharp youngster is straight, in the time of cell in the piping and druming culturing bottle; Supporting use rubber pipette bulb; The suction nozzle of dropper is parallel operation with the bottom surface of culturing bottle, and having caused the bottom surface of piping and druming like this is that the inclined-plane goes down, and the piping and druming zone is excessive; The cell one that is in culturing bottle bottom blows and a sheet ofly all falls down, and can not become single cell suspension.In addition, shoulder place on two of culturing bottle, straight tip can not blow to, and causes the cell at two shoulder places to go down to posterity, thereby can influence going down to posterity of whole bottle cell.If piping and druming time at the bottom of the body of suction pipe and the parallel plane words of piping and druming, liquid can impact bottle, is easy to generate foam, bubble break back moment is huge for the surging force of cell, has a strong impact on the growth of cell.
Summary of the invention
The purpose of the utility model is for overcoming the deficiency of above-mentioned prior art, a kind of novel passage device being provided.
For realizing above-mentioned purpose, the utility model adopts following technical proposals:
A kind of novel passage device, it comprises rubber pipette bulb and dropper main body, and the rear end of dropper main body links to each other with said rubber pipette bulb, and the front end of dropper main body has suction nozzle, and said suction nozzle has crooked shape.
The terminal crooked back of said suction nozzle is 45 ° with the angle of dropper main body.
Method of use: hand-held supporting rubber pipette bulb, dropper is put in the bottle, draw the nutrient solution in the culturing bottle, blow and beat facing to the cell of culturing bottle bottom and go down to posterity.
The beneficial effect of the utility model is, the curved suction nozzle design of 45 ° of knuckles is made in (1), make suction nozzle face toward bottle at the bottom of direction, be convenient to piping and druming to the bottle floor cells, more be prone to become single cell suspension; In addition,, can alleviate the dynamics of finger presses, reduce the foam that produces at the bottom of liquid fiercely impacts bottle, thereby also reduce the damage of foam pair cell owing to be direction at the bottom of suction nozzle faces toward bottle; (2) the curved suction nozzle of making 30 ° of knuckles designs, and can blow down the cell of two last shoulders of culturing bottle come, and has avoided straight dropper can't blow to two shortcomings that go up shoulder of culturing bottle; (3) the curved suction nozzle of making 45 ° of knuckles designs, and the object suction that will be bonded at easily on the culturing bottle inwall scrapes off.
Description of drawings
Fig. 1 is the utility model structural representation.
1. rubber pipette bulbs wherein, 2. dropper main body, 3. suction nozzle, α. angle.
Embodiment
Below in conjunction with accompanying drawing and embodiment the utility model is further specified.
As shown in Figure 1, a kind of novel passage device, it comprises rubber pipette bulb 1 and dropper main body 2, and the rear end of dropper main body 2 links to each other with said rubber pipette bulb 1, and the front end of dropper main body 2 has suction nozzle 3, and said suction nozzle 3 has crooked shape.The medullary ray of crooked back suction nozzle 3 ends and the angle of dropper main body 2 medullary rays are 45 °.
Claims (2)
1. novel passage device, it comprises rubber pipette bulb and dropper main body, and the rear end of dropper main body links to each other with said rubber pipette bulb, and the front end of dropper main body has suction nozzle, it is characterized in that, and said suction nozzle has crooked shape.
2. novel passage device as claimed in claim 1 is characterized in that, the terminal crooked back of said suction nozzle is 45 ° with the angle of dropper main body.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 201220073422 CN202440493U (en) | 2012-03-01 | 2012-03-01 | Novel cell passage device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 201220073422 CN202440493U (en) | 2012-03-01 | 2012-03-01 | Novel cell passage device |
Publications (1)
Publication Number | Publication Date |
---|---|
CN202440493U true CN202440493U (en) | 2012-09-19 |
Family
ID=46821947
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 201220073422 Expired - Fee Related CN202440493U (en) | 2012-03-01 | 2012-03-01 | Novel cell passage device |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN202440493U (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105238689A (en) * | 2015-09-29 | 2016-01-13 | 中牧实业股份有限公司 | Cell blower and cell subculture method based on same |
CN111961589A (en) * | 2020-08-12 | 2020-11-20 | 江苏信安佳医疗科技有限公司 | Automatic liquid changing device for three-dimensional organoid sphere culture |
-
2012
- 2012-03-01 CN CN 201220073422 patent/CN202440493U/en not_active Expired - Fee Related
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105238689A (en) * | 2015-09-29 | 2016-01-13 | 中牧实业股份有限公司 | Cell blower and cell subculture method based on same |
CN111961589A (en) * | 2020-08-12 | 2020-11-20 | 江苏信安佳医疗科技有限公司 | Automatic liquid changing device for three-dimensional organoid sphere culture |
CN111961589B (en) * | 2020-08-12 | 2023-10-31 | 江苏信安佳医疗科技有限公司 | Automatic liquid changing device for three-dimensional organoid sphere culture |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C17 | Cessation of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120919 Termination date: 20130301 |