CN202437782U - Fat storing barrel for separating primary adipose-derived stem cells and liposuction machine comprising same - Google Patents
Fat storing barrel for separating primary adipose-derived stem cells and liposuction machine comprising same Download PDFInfo
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- CN202437782U CN202437782U CN 201220011745 CN201220011745U CN202437782U CN 202437782 U CN202437782 U CN 202437782U CN 201220011745 CN201220011745 CN 201220011745 CN 201220011745 U CN201220011745 U CN 201220011745U CN 202437782 U CN202437782 U CN 202437782U
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- depot fat
- bucket
- fat bucket
- depot
- bottom opening
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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Abstract
The utility model discloses a fat storing barrel for separating primary adipose-derived stem cells and a liposuction machine comprising the same. The fat storing barrel comprises a body for receiving and containing adipose tissue, the body is provided with a top portion, lateral walls and a bottom portion, wherein the top portion is provided with a top opening, the adipose tissue and a reagent for separating the primary adipose-derived stem cells are injected from the top opening to the body, the bottom portion is provided with at least one bottom opening which can be closed, and contained objects in the body are discharged from the bottom opening. The fat storing barrel for separating the primary adipose-derived stem cells does not need secondary change of a container, is convenient to operate, avoids pollution possibly caused by an intermediate link, improves accuracy of the scientific research, saves scientific research cost, reduces experiment rubbishes and is favorable for environmental protection.
Description
Technical field
This utility model relates to a kind of depot fat bucket, particularly relates to a kind of depot fat bucket that is used for separating from fatty tissue former fat subsitutes stem cell, and this utility model also relates to a kind of fat suction machine that comprises the depot fat bucket.
Background technology
At plastic surgery's medical domain, the extensive patients that appears as of fat absorption method has been brought healthy and beautiful with the personage that likes to be beautiful.The negative pressure fat suction machine is the indispensable equipment of this operation, and fat is inhaled in the depot fat bucket of this equipment in the art, and these are closed a large amount of former fat subsitutes stem cell in fat of sucking-off, is the perfect cell source of the former fat subsitutes stem cell of research.Conventional at present depot fat bucket only is a common tub; Extract former fat subsitutes stem cell and will pass through a plurality of washings, digestion and separating step; Could realize after shifting several containers that this causes the extraction of former fat subsitutes stem cell to be wasted time and energy, and possibly have potential pollution.
The utility model content
This utility model just is being based on top consideration and is making.
According to the one side of this utility model, a kind of depot fat bucket is proposed, utilize this depot fat bucket to isolate former fat subsitutes stem cell from fatty tissue; Wherein, the depot fat bucket comprises the body that receives and hold fatty tissue, and this body has top, sidewall and bottom; Wherein, the top has open top, and fatty tissue is injected into body with the reagent that is used for separating former fat subsitutes stem cell from open top; And; The bottom has at least one bottom opening, and this at least one bottom opening can be closed, and intrinsic contents are discharged from this at least one bottom opening.
Preferably, the depot fat bucket also comprises lid, and open top is arranged on said covering.
Preferably, said at least one bottom opening is arranged on the basal surface of body.
Preferably, said at least one bottom opening is arranged on the position that the sidewall of body engages with the basal surface of body.
Preferably, the sidewall of body has constant lateral dimension.
Preferably, the sidewall of body has the lateral dimension that from the top to the bottom, reduces gradually.
Preferably, the sidewall of body has constant lateral dimension near the top, and near the bottom, has the lateral dimension that reduces gradually.
Preferably, the body of depot fat bucket is processed by the transparent/translucent resin, and it is provided with the scale layout.
Preferably, the body of depot fat bucket is made of metal, and it is provided with watch window and scale is arranged.
Preferably, the catch opened/closed of said at least one bottom opening through being handled.
Preferably, said at least one bottom opening is connected to valve.
According to this utility model on the other hand, a kind of fat suction machine that comprises above-mentioned depot fat bucket is also proposed.
According to this utility model; Utilize fatty tissue density little, always float over the characteristics on aqueous solution upper strata, at least one bottom opening is set in the bottom of depot fat bucket; Just can realize only using a container, accomplish the operation of washing, digesting and extracting former fat subsitutes stem cell easily.Change container owing to need not secondary, so both made things convenient for operation, avoided again changing the pollution that container possibly cause, improved the accuracy of scientific research, also saved scientific research cost simultaneously, reduced the generation of medical waste, be beneficial to environmental protection.
Description of drawings
Fig. 1 is the sketch map of first embodiment of the depot fat bucket of this utility model.
Fig. 2 is the sketch map of second embodiment of the depot fat bucket of this utility model.
Fig. 3 is the sketch map of the 3rd embodiment of the depot fat bucket of this utility model.
Fig. 4 is the sketch map of the 4th embodiment of the depot fat bucket of this utility model.
The specific embodiment
Describe the depot fat bucket that is used for separating former fat subsitutes stem cell of this utility model with reference to the accompanying drawings in detail from fatty tissue.
Fig. 1 is the sketch map of first embodiment of the depot fat bucket of this utility model.Depot fat bucket 10 according to this embodiment comprises body 11, and body 11 is used for receiving and holding fatty tissue, for example in fat absorption method with the fatty tissue of negative pressure fat suction machine sucking-off.Body 11 can be an Any shape, for example cylindrical shape, square etc.This body 11 has top, sidewall and bottom.The top of body 11 can be open; Also can have and cover 12; Be used to cover the cavity of body 11, lid 12 is provided with opening 13, and fatty tissue is injected into body with the reagent that is used for separating former fat subsitutes stem cell through the top of opening or the opening 13 that covers on 12.In this embodiment, the sidewall of body 11 is arranged essentially parallel to the longitudinal axis of body, that is, body 11 has constant lateral dimension.The basal surface of body 11 is provided with the closeable bottom opening 14 that is used to discharge intrinsic contents.Though this embodiment shows a bottom opening 14, also a plurality of bottom openings 14 can be set in position as the case may be, so that discharge the contents that are positioned at body 11 bottoms smoothly and effectively.
Fig. 2 is the sketch map of second embodiment of the depot fat bucket of this utility model.Depot fat bucket 20 according to this embodiment comprises body 21, and different with the depot fat bucket of first embodiment 10 is, at least one closeable bottom opening 24 is arranged on the position that the sidewall of body 21 engages with basal surface.
Fig. 3 is the sketch map of the 3rd embodiment of the depot fat bucket of this utility model.Depot fat bucket 30 according to this embodiment comprises body 31, and different with the depot fat bucket of first embodiment 10 is that the lateral dimension of body 31 (width) reduces from the top to the bottom gradually.According to this embodiment, the contents in the depot fat bucket 30 can controllably, accurately be discharged through bottom opening.
Fig. 4 is the sketch map of the 4th embodiment of the depot fat bucket of this utility model.Depot fat bucket 40 according to this embodiment comprises body 41, and different with the depot fat bucket of first embodiment 10 is that the lateral dimension of body 41 is constant near the top, and near the bottom, reduces gradually.According to this embodiment, can be under the situation of body unobstructive, make in the depot fat bucket 40 contents through bottom opening controllably, accurately discharge.
In above-mentioned each embodiment, closeable bottom opening can be through the variety of way realization, for example; Operation rotary switch or toggle switch make a catch cover/open bottom opening; Again for example, a valving is connected in bottom opening, realizes the opened/closed of bottom opening through the operation of valve.Be provided with in the bottom of depot fat bucket under the situation of outstanding downwards switch or valve, can prop or make the sidewall of body and bottom to surround a recess in the depot fat bucket with carriage selectively with ccontaining said switch or valve.
In above-mentioned each embodiment, body can be processed by resin or metal, and selectable lid also can be processed by resin or metal.Preferably, body is processed by the resin of transparent/translucent; Preferably, the sidewall of body is provided with the scale layout.When body is made of metal, watch window and scale can be set on the sidewall of body arranges.
The method of using above-mentioned depot fat bucket to isolate fat stem cell is described below.
At first, fatty tissue is collected in the body of depot fat bucket through the open top of depot fat bucket.
Then, the open top through the depot fat bucket adds in the body of depot fat bucket and the isopyknic phosphate buffer of fatty tissue (PBS), and fully swings and wash.Afterwards, left standstill 1 minute, at this moment solution will be divided into two-layerly in the bucket, and lower floor is a phosphate buffer, and the upper strata is a yellow fat tissue, owing to closed erythrocyte in the fatty tissue, phosphate buffer washing afterwards color becomes pale red.Then, open the bottom opening of depot fat bucket, emit the phosphate buffer of lower floor.
Repeat above step, for example 3-5 time, till phosphate buffer does not have obvious variable color.
Then, the open top through the depot fat bucket adds in the body of depot fat bucket and the isopyknic type i collagen enzymic digestion of fatty tissue liquid, and the depot fat bucket is placed 37 ℃ of constant temperature shaking tables, digestion a period of time, for example 30 minutes.Afterwards, left standstill 1 minute, at this moment solution will be divided into two-layerly in the bucket, and lower floor is a type i collagen enzymic digestion liquid, digests the human adipose-derived stem cell that and promptly is deposited in wherein, and the upper strata is the yellow fat tissue, can see that the part fatty tissue is digested, and the fat deposit volume reduces.Subsequently, open the bottom opening of depot fat bucket, emit lower floor's celliferous type i collagen enzymic digestion liquid to centrifuge tube, then the centrifugalize human adipose-derived stem cell.After this, the open top through the depot fat bucket adds in the depot fat bucket and the isopyknic type i collagen enzymic digestion of fatty tissue liquid again, and repeats above digestion step 3-5 time, up to fatty tissue digestion totally.
At last, collect the centrifugal human adipose-derived stem cell that obtains, place culture dish to cultivate, increase, be used for subsequent experimental etc. after conventional method purification, the evaluation.
Phosphate buffer (PBS) is the level pad that is configured to by an amount of sodium chloride, sodium hydrogen phosphate, potassium dihydrogen phosphate, potassium chloride, and pH value is 7.4, and concrete prescription is NaCl:8 gram, KCl:0.20 gram, Na
2HPO
4: 3.49 grams, KH
2PO
4: 0.20 gram, add in the distilled water, standardize solution is 1 liter, 0.22 micron membrane filtration degerming.
Commercially available type i collagen enzyme (powdery) is dissolved in the phosphate buffer with mass fraction 0.1%, and 0.22 micron membrane filtration degerming promptly becomes type i collagen enzymic digestion liquid, is used to digest fatty tissue.
The complicated steps of conventional enzyme digestion separation of human fat stem cell all will be changed container No. one time after washing, digest fatty tissue each time, has both increased the risk of operating procedure and pollution, has also caused unnecessary waste.Adopt the depot fat bucket of this utility model can greatly simplify the step of separation human adipose-derived stem cell of former generation, and practice thrift the consumption of experiment container, reduced the experiment generation of waste, help environmental protection.
Though combined specific embodiment to describe this utility model in detail, apparently, under the condition of spirit that does not depart from this utility model and scope, those skilled in the art can make various changes and modification.
Claims (12)
1. a depot fat bucket is isolated former fat subsitutes stem cell from fatty tissue, it is characterized in that; Said depot fat bucket comprises the body that receives and hold fatty tissue, and this body has top, sidewall and bottom, wherein; Said top has open top; Fatty tissue is injected into body with the reagent that is used for separating former fat subsitutes stem cell from said open top, and said bottom has at least one bottom opening; This at least one bottom opening can be closed, and intrinsic contents are discharged from this at least one bottom opening.
2. according to the depot fat bucket of claim 1, it is characterized in that said depot fat bucket also comprises lid, said open top is arranged on said covering.
3. according to the depot fat bucket of claim 1, it is characterized in that said at least one bottom opening is arranged on the basal surface of said body.
4. according to the depot fat bucket of claim 1, it is characterized in that said at least one bottom opening is arranged on the position that the sidewall of said body engages with the basal surface of said body.
5. according to each depot fat bucket among the claim 1-3, it is characterized in that the sidewall of said body has constant lateral dimension.
6. according to each depot fat bucket among the claim 1-3, it is characterized in that the sidewall of said body has the lateral dimension that reduces gradually to said bottom from said top.
7. according to each depot fat bucket among the claim 1-3, it is characterized in that the sidewall of said body has constant lateral dimension near said top, and near said bottom, has the lateral dimension that reduces gradually.
8. according to each depot fat bucket among the claim 1-4, it is characterized in that the body of said depot fat bucket is processed by the transparent/translucent resin, and it is provided with scale and arranges.
9. according to each depot fat bucket among the claim 1-4, it is characterized in that the body of said depot fat bucket is made of metal, and it is provided with watch window and scale is arranged.
10. according to each depot fat bucket among the claim 1-4, it is characterized in that the catch opened/closed of said at least one bottom opening through being handled.
11., it is characterized in that said at least one bottom opening is connected to valve according to each depot fat bucket among the claim 1-4.
12. a fat suction machine comprises according to each depot fat bucket among the claim 1-11.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220011745 CN202437782U (en) | 2012-01-12 | 2012-01-12 | Fat storing barrel for separating primary adipose-derived stem cells and liposuction machine comprising same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220011745 CN202437782U (en) | 2012-01-12 | 2012-01-12 | Fat storing barrel for separating primary adipose-derived stem cells and liposuction machine comprising same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202437782U true CN202437782U (en) | 2012-09-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220011745 Expired - Fee Related CN202437782U (en) | 2012-01-12 | 2012-01-12 | Fat storing barrel for separating primary adipose-derived stem cells and liposuction machine comprising same |
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| Country | Link |
|---|---|
| CN (1) | CN202437782U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103203043A (en) * | 2012-01-12 | 2013-07-17 | 刘云松 | Adipose storage barrel for separating primary adipose-derived stem cells, and application method of adipose storage barrel |
-
2012
- 2012-01-12 CN CN 201220011745 patent/CN202437782U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103203043A (en) * | 2012-01-12 | 2013-07-17 | 刘云松 | Adipose storage barrel for separating primary adipose-derived stem cells, and application method of adipose storage barrel |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120919 Termination date: 20150112 |
|
| EXPY | Termination of patent right or utility model |