CN103203043A - Adipose storage barrel for separating primary adipose-derived stem cells, and application method of adipose storage barrel - Google Patents
Adipose storage barrel for separating primary adipose-derived stem cells, and application method of adipose storage barrel Download PDFInfo
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- CN103203043A CN103203043A CN2012100080825A CN201210008082A CN103203043A CN 103203043 A CN103203043 A CN 103203043A CN 2012100080825 A CN2012100080825 A CN 2012100080825A CN 201210008082 A CN201210008082 A CN 201210008082A CN 103203043 A CN103203043 A CN 103203043A
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- depot fat
- fat bucket
- bucket
- adipose
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Abstract
The invention discloses an adipose storage barrel for separating primary adipose-derived stem cells from adipose tissues, an application method of the adipose storage barrel, and a liposuction machine. The adipose storage barrel comprises a body for receiving and containing the adipose tissues, wherein the body is provided with a top part, a side wall and a bottom part; the top part is provided with a top opening, and the adipose tissues and a reagent for separating the primary adipose-derived stem cells are poured into the body through the top opening; and moreover, at least one bottom opening is formed in the bottom, can be closed and is used for discharging containing objects in the body. The adipose storage barrel is used for separating the primary adipose-derived stem cells, does not need secondary container changing, and is not only convenient to operate but also prevents intermediate links from causing possible contamination, so that the accuracy of scientific researches is improved, the scientific research cost is also lowered, the produced experimental wastes are reduced, and the adipose storage barrel is beneficial to environmental protection.
Description
Technical field
The present invention relates to a kind of depot fat bucket and using method thereof, particularly relate to a kind ofly for depot fat bucket and the using method thereof of separating former fat subsitutes stem cell from fatty tissue, the invention still further relates to a kind of fat suction machine that comprises the depot fat bucket.
Background technology
At plastic surgery's medical domain, the extensive patients that appears as of fat absorption method has been brought healthy and beautiful with the personage that likes to be beautiful.The negative pressure fat suction machine is the indispensable equipment of this operation, and fat is inhaled in the depot fat bucket of this equipment in the art, contains a large amount of former fat subsitutes stem cell in these fat that are sucked out, and is the perfect cell source of the former fat subsitutes stem cell of research.Conventional at present depot fat bucket only is a common tub, extract former fat subsitutes stem cell and will pass through a plurality of washings, digestion and separating step, could realize after shifting several containers that this causes the extraction of former fat subsitutes stem cell to be wasted time and energy, and may have potential pollution.
Summary of the invention
The present invention just is being based on top consideration and is making.
According to an aspect of the present invention, comprise for the body that receives and hold fatty tissue for the depot fat bucket of isolating former fat subsitutes stem cell from fatty tissue, this body has top, sidewall and bottom, wherein, the top has open top, fatty tissue and be injected into the body from this open top for separating of the reagent of former fat subsitutes stem cell, and, the bottom has at least one bottom opening, and this at least one bottom opening is closeable, is used for discharging intrinsic contents.
According to a further aspect in the invention, a kind of fat suction machine that comprises above-mentioned depot fat bucket is proposed.
According to another aspect of the invention, propose a kind of using method of above-mentioned depot fat bucket, comprise the steps: to collect fatty tissue in the body of depot fat bucket by the open top of depot fat bucket; Open top by the depot fat bucket adds and the isopyknic phosphate buffer of fatty tissue in the body of depot fat bucket, fully swings and washes, and leaves standstill afterwards; Open at least one bottom opening of depot fat bucket, emit the phosphate buffer of lower floor; Repeat above-mentioned steps, till phosphate buffer does not have obvious variable color; Open top by the depot fat bucket adds and the isopyknic type i collagen enzymic digestion of fatty tissue liquid in the body of depot fat bucket, and the depot fat bucket is placed the constant temperature shaking table, digests, and leaves standstill afterwards; Open at least one bottom opening of depot fat bucket, emit lower floor's celliferous type i collagen enzymic digestion liquid to centrifuge tube, then the centrifugalize human adipose-derived stem cell.
According to the present invention, utilize fatty tissue density little, always float over the characteristics on aqueous solution upper strata, in the bottom of depot fat bucket at least one bottom opening is set, just can realize only using a container, finish washing easily, digest and extract the operation of former fat subsitutes stem cell.Change container owing to need not secondary, so both made things convenient for operation, avoided again changing the pollution that container may cause, improved the accuracy of scientific research, also saved scientific research cost simultaneously, reduced the generation of medical waste, be beneficial to environmental protection.
Description of drawings
Fig. 1 is the sketch map of first embodiment of depot fat bucket of the present invention.
Fig. 2 is the sketch map of second embodiment of depot fat bucket of the present invention.
Fig. 3 is the sketch map of the 3rd embodiment of depot fat bucket of the present invention.
Fig. 4 is the sketch map of the 4th embodiment of depot fat bucket of the present invention.
The specific embodiment
Describe in detail of the present invention for the depot fat bucket that separates former fat subsitutes stem cell from fatty tissue with reference to the accompanying drawings.
Fig. 1 is the sketch map of first embodiment of depot fat bucket of the present invention.Depot fat bucket 10 according to this embodiment comprises body 11, and body 11 is used for receiving and holding fatty tissue, for example uses the fatty tissue of negative pressure fat suction machine sucking-off in fat absorption method.Body 11 can be Any shape, for example cylindrical shape, square etc.This body 11 has top, sidewall and bottom.The top of body 11 can be open, also can have and cover 12, be used for to cover the cavity of body 11, lid 12 is provided with opening 13, fatty tissue and be injected in the body by open top or the opening 13 that covers on 12 for separating of the reagent of former fat subsitutes stem cell.In this embodiment, the sidewall of body 11 is arranged essentially parallel to the longitudinal axis of body, that is, body 11 has constant lateral dimension.The basal surface of body 11 is provided with for the closeable bottom opening 14 of discharging intrinsic contents.Though this embodiment shows a bottom opening 14, also a plurality of bottom openings 14 can be set in position as the case may be, in order to discharge the contents that are positioned at body 11 bottoms smoothly and effectively.
Fig. 2 is the sketch map of second embodiment of depot fat bucket of the present invention.Depot fat bucket 20 according to this embodiment comprises body 21, and different with the depot fat bucket 10 of first embodiment is, at least one closeable bottom opening 24 is arranged on the position that the sidewall of body 21 engages with basal surface.
Fig. 3 is the sketch map of the 3rd embodiment of depot fat bucket of the present invention.According to the depot fat bucket 30 of this embodiment comprise the depot fat bucket 10 of body 31 and first embodiment different be that the lateral dimension (width) of body 31 reduces from the top to the bottom gradually.According to this embodiment, the contents in the depot fat bucket 30 can controllably, accurately be discharged by bottom opening.
Fig. 4 is the sketch map of the 4th embodiment of depot fat bucket of the present invention.Depot fat bucket 40 according to this embodiment comprises body 41, and different with the depot fat bucket 10 of first embodiment is that the lateral dimension of body 41 is constant near the top, and reduces gradually near the bottom.According to this embodiment, can be under the situation of body unobstructive, make in the depot fat bucket 40 contents by bottom opening controllably, accurately discharge.
In the various embodiments described above, closeable bottom opening can realize by variety of way, for example, operation rotary switch or toggle switch make a catch cover/open bottom opening, again for example, a valving is connected in bottom opening, realizes the opened/closed of bottom opening by the operation of valve.Be provided with in the bottom of depot fat bucket under the situation of outstanding downwards switch or valve, can prop or make the sidewall of body and bottom to surround a recess with ccontaining described switch or valve in the depot fat bucket with carriage selectively.
In the various embodiments described above, body can be made by resin or metal, and selectable lid also can be made by resin or metal.Preferably, body is made by the resin of transparent/translucent; Preferably, the sidewall of body is provided with the scale layout.When body is made of metal, watch window and scale can be set at the sidewall of body arranges.
The method of using above-mentioned depot fat bucket to isolate fat stem cell is described below.
At first, fatty tissue is collected in the body of depot fat bucket by the open top of depot fat bucket.
Then, the open top by the depot fat bucket adds and the isopyknic phosphate buffer of fatty tissue (PBS) in the body of depot fat bucket, and fully swings and wash.Afterwards, left standstill 1 minute, at this moment solution will be divided into two-layerly in the bucket, and lower floor is phosphate buffer, and the upper strata is yellow fat tissue, owing to contain erythrocyte in the fatty tissue, phosphate buffer washing afterwards color becomes pale red.Then, open the bottom opening of depot fat bucket, emit the phosphate buffer of lower floor.
Repeat above step, for example 3-5 time, till phosphate buffer does not have obvious variable color.
Then, the open top by the depot fat bucket adds and the isopyknic type i collagen enzymic digestion of fatty tissue liquid in the body of depot fat bucket, and the depot fat bucket is placed 37 ℃ of constant temperature shaking tables, digestion a period of time, for example 30 minutes.Afterwards, left standstill 1 minute, at this moment solution will be divided into two-layerly in the bucket, and lower floor is type i collagen enzymic digestion liquid, digests the human adipose-derived stem cell that and namely is deposited in wherein, and the upper strata is the yellow fat tissue, can see that the part fatty tissue is digested, and the fat deposit volume reduces.Subsequently, open the bottom opening of depot fat bucket, emit lower floor's celliferous type i collagen enzymic digestion liquid to centrifuge tube, then the centrifugalize human adipose-derived stem cell.After this, the open top by the depot fat bucket adds and the isopyknic type i collagen enzymic digestion of fatty tissue liquid in the depot fat bucket again, and repeats above digestion step 3-5 time, up to fatty tissue digestion totally.
At last, collect the centrifugal human adipose-derived stem cell that obtains, place culture dish to cultivate, increase, conventional method purification, evaluation back are used for subsequent experimental etc.
Phosphate buffer (PBS) is the level pad that is configured to by an amount of sodium chloride, sodium hydrogen phosphate, potassium dihydrogen phosphate, potassium chloride, and pH value is 7.4, and concrete prescription is NaCl:8 gram, KCl:0.20 gram, Na
2HPO
4: 3.49 grams, KH
2PO
4: 0.20 gram, add in the distilled water, standardize solution is 1 liter, 0.22 micron membrane filtration degerming.
Commercially available type i collagen enzyme (powdery) is dissolved in the phosphate buffer with mass fraction 0.1%, and 0.22 micron membrane filtration degerming namely becomes type i collagen enzymic digestion liquid, is used for the digestion fatty tissue.
The step complexity of conventional enzyme digestion separation of human fat stem cell all will be changed container No. one time after washing, digest fatty tissue each time, has both increased the risk of operating procedure and pollution, has also caused unnecessary waste.Adopt depot fat bucket of the present invention can greatly simplify the step of separation human adipose-derived stem cell of former generation, and save the consumption of experiment container, reduced the experiment generation of waste, be conducive to environmental protection.
Though described the present invention in conjunction with specific embodiments in detail, apparently, under condit without departing from the spirit and scope of the present invention, those skilled in the art can make various changes and modification.
Claims (11)
1. depot fat bucket, be used for isolating former fat subsitutes stem cell from fatty tissue, comprise that this body has top, sidewall and bottom, wherein for the body that receives and hold fatty tissue, the top has open top, fatty tissue and be injected into the body from this open top for separating of the reagent of former fat subsitutes stem cell, and the bottom has at least one bottom opening, this at least one bottom opening is closeable, is used for discharging intrinsic contents.
2. according to the depot fat bucket of claim 1, wherein, the depot fat bucket also comprises lid, and described open top is arranged on and covers.
3. according to the depot fat bucket of claim 1, wherein, described at least one bottom opening is arranged on the basal surface of body or is arranged on the position that the sidewall of body engages with basal surface.
4. according to each depot fat bucket among the claim 1-3, wherein, the sidewall of body has constant lateral dimension or have the lateral dimension that reduces gradually from the top to the bottom.
5. according to each depot fat bucket among the claim 1-3, wherein, the sidewall of body has constant lateral dimension near the top, and has the lateral dimension that reduces gradually near the bottom.
6. according to each depot fat bucket among the claim 1-3, wherein, the body of depot fat bucket is made by the transparent/translucent resin, and it is provided with scale and arranges.
7. according to each depot fat bucket among the claim 1-3, wherein, the body of depot fat bucket is made of metal, and it is provided with watch window and scale is arranged.
8. according to each depot fat bucket among the claim 1-3, wherein, described at least one bottom opening is by steerable catch opened/closed.
9. according to each depot fat bucket among the claim 1-3, wherein, described at least one bottom opening is connected to valve.
10. fat suction machine comprises according to each depot fat bucket among the claim 1-9.
11. the using method of each depot fat bucket comprises the steps: among the claim 1-10
Collect fatty tissue in the body of depot fat bucket by the open top of depot fat bucket;
Open top by the depot fat bucket adds and the isopyknic phosphate buffer of fatty tissue in the body of depot fat bucket, fully swings and washes, and leaves standstill afterwards;
Open at least one bottom opening of depot fat bucket, emit the phosphate buffer of lower floor;
Repeat above-mentioned steps, till phosphate buffer does not have obvious variable color;
Open top by the depot fat bucket adds and the isopyknic type i collagen enzymic digestion of fatty tissue liquid in the body of depot fat bucket, and the depot fat bucket is placed 37 ℃ of constant temperature shaking tables, digests, and leaves standstill afterwards;
Open at least one bottom opening of depot fat bucket, emit lower floor's celliferous type i collagen enzymic digestion liquid to centrifuge tube, then the centrifugalize human adipose-derived stem cell.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100080825A CN103203043A (en) | 2012-01-12 | 2012-01-12 | Adipose storage barrel for separating primary adipose-derived stem cells, and application method of adipose storage barrel |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012100080825A CN103203043A (en) | 2012-01-12 | 2012-01-12 | Adipose storage barrel for separating primary adipose-derived stem cells, and application method of adipose storage barrel |
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| CN103203043A true CN103203043A (en) | 2013-07-17 |
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| CN2012100080825A Pending CN103203043A (en) | 2012-01-12 | 2012-01-12 | Adipose storage barrel for separating primary adipose-derived stem cells, and application method of adipose storage barrel |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109415675A (en) * | 2016-03-17 | 2019-03-01 | 辛诺瓦生命科学股份有限公司 | Use shock wave or the method for mechanical shock separation, dissociation and/or lytic cell |
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| CA2141573A1 (en) * | 1992-07-31 | 1994-02-17 | Paul G. Alchas | Device and method for processing fat tissue to produce endothelial cell product |
| CN2616216Y (en) * | 2003-02-08 | 2004-05-19 | 杨军庭 | Self living body fat cell extractor |
| CN1842589A (en) * | 2003-06-25 | 2006-10-04 | 马克罗珀尔生物外科公司 | Systems and methods for separating and concentrating regenerative cells from tissue |
| US20090287190A1 (en) * | 2005-03-23 | 2009-11-19 | Shippert Ronald D | Tissue transfer method and apparatus |
| US20110251041A1 (en) * | 2010-04-12 | 2011-10-13 | Biomet Biologics, Llc | Method and Apparatus for Separating a Material |
| CN202437782U (en) * | 2012-01-12 | 2012-09-19 | 刘云松 | Fat storing barrel for separating primary adipose-derived stem cells and liposuction machine comprising same |
-
2012
- 2012-01-12 CN CN2012100080825A patent/CN103203043A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2141573A1 (en) * | 1992-07-31 | 1994-02-17 | Paul G. Alchas | Device and method for processing fat tissue to produce endothelial cell product |
| CN2616216Y (en) * | 2003-02-08 | 2004-05-19 | 杨军庭 | Self living body fat cell extractor |
| CN1842589A (en) * | 2003-06-25 | 2006-10-04 | 马克罗珀尔生物外科公司 | Systems and methods for separating and concentrating regenerative cells from tissue |
| US20090287190A1 (en) * | 2005-03-23 | 2009-11-19 | Shippert Ronald D | Tissue transfer method and apparatus |
| US20110251041A1 (en) * | 2010-04-12 | 2011-10-13 | Biomet Biologics, Llc | Method and Apparatus for Separating a Material |
| CN202437782U (en) * | 2012-01-12 | 2012-09-19 | 刘云松 | Fat storing barrel for separating primary adipose-derived stem cells and liposuction machine comprising same |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109415675A (en) * | 2016-03-17 | 2019-03-01 | 辛诺瓦生命科学股份有限公司 | Use shock wave or the method for mechanical shock separation, dissociation and/or lytic cell |
| US11866732B2 (en) | 2016-03-17 | 2024-01-09 | Synova Life Sciences, Inc. | Separation, dissociation and/or disaggregation of cells using shockwaves or mechanical impacts |
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Application publication date: 20130717 |