CN202297576U - Microfluidic device for sperm optimization - Google Patents
Microfluidic device for sperm optimization Download PDFInfo
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- CN202297576U CN202297576U CN2011203698572U CN201120369857U CN202297576U CN 202297576 U CN202297576 U CN 202297576U CN 2011203698572 U CN2011203698572 U CN 2011203698572U CN 201120369857 U CN201120369857 U CN 201120369857U CN 202297576 U CN202297576 U CN 202297576U
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Abstract
The utility model discloses a microfluidic device for sperm optimization, comprising a piece of slide glass provided with a buffer solution sample loading pool, a sample detecting pool and a sample collecting pool, and a support substrate for supporting the slide glass, wherein a buffer solution connecting channel is disposed between the buffer solution sample loading pool and the sample detecting pool; a sample acting pool is disposed in the sample detecting pool; one or more screening channels are disposed between the sample detecting pool and the sample acting pool; at least one screening channel is disposed at one side of the sample detecting pool connected with the sample collecting pool; and a sample collecting channel is disposed between the sample detecting pool and the sample collecting pool. The microfluidic device for sperm optimization disclosed by the utility model can simulate a physiological status that semen is interacted with cervical mucus in the sample acting pool, and then screen sperms high in vitality and fast in linear motion speed by disposing the sample acting pool, thus avoiding the injury on sperms caused by centrifugation treatment and improving the quality of sperms. The microfluidic device for sperm optimization disclosed by the utility model can be used as tool for assisted reproductive technology.
Description
Technical field
The utility model relates to a kind of sperm that is used for and preferably installs.
Background technology
Research shows that the sickness rate of infertility has the trend that rises year by year, and at the sickness rate average out to 7%-10% of Chinese infertility, wherein male factor accounts for 30%, and sperm quality is to influence one of key factor of becoming pregnant.Auxiliary procreation technology provides an approach for solving this medical problem.
The micro-fluidic chip technology is to be integrated into basic operation units such as the specimen preparation of biological, chemistry, medical analysis process, reaction, separation, detection on the chip of a micro-meter scale, accomplishes automatically and analyzes whole process.Because it in the great potential in fields such as biology, chemistry, medical science, has developed into the brand-new research field of subject crossing such as a biology, chemistry, medical science, fluid, electronics, material, machinery.
Yet the prerequisite of implementing various auxiliary procreation technologies is from seminal fluid, to obtain high-quality sperm, and need the white corpuscle in the seminal fluid, epithelial cell etc. be separated with the high quality sperm.The staple of judging the high quality sperm is the vigor of sperm, the point-to-point speed of sperm, the normal morphology rate of sperm and the dna damage rate of sperm.At present, micro-fluidic chip can not be simulated the sperm assorting room under the physiological status, and the assorting room time is long; Need centrifugal treating, too much centrifugation step is prone to produce oxyradical, causes the sperm DNA peroxide injury; Cause that sperm quality reduces, and influences the success ratio of auxiliary procreation technology.
The utility model content
The technical problem that the utility model will solve provides a kind of sperm preferred embodiment, and it filters out high-quality sperm naturally, reduces and in screening process, sperm DNA is damaged.
The utility model solves the technical scheme that its technical problem adopted: the preferred micro fluidic device of a kind of sperm; Comprise slide glass and the substrate that supports slide glass; Be provided with damping fluid addition pool, pattern detection pond and sample collection pond at slide glass; Be provided with the damping fluid connecting passage between said damping fluid addition pool and pattern detection pond, be provided with sample effect pond in the said pattern detection pond, be provided with one between said pattern detection pond and sample effect pond and perhaps screen passage more than one; Have at least a bar grizzl(e)y gating road to be located at the side that the pattern detection pond is connected with the sample collection pond, be provided with the sample collection passage between said pattern detection pond and sample collection pond.
Further as the improvement of the utility model technical scheme, said slide glass and substrate are fixed through sealing-in.
Further as the improvement of the utility model technical scheme, said slide glass is processed by the YSR 3286 material, and substrate is processed by glass or quartzy slide or YSR 3286 material.
The beneficial effect of the utility model: the utility model is provided with sample effect pond; And add seminal fluid and the words that are in women of child-bearing age's's onset of ovulation cervical mucus in sample effect pond; Can simulate the interactional physiological status of seminal fluid and cervical mucus; Utilize the viscosity sperm and the white corpuscle epithelial cell in the seminal fluid that vigor is poor slightly of cervical mucus to be trapped in the sample effect pond; Filter out high vigor, the fast sperm of point-to-point speed, avoided the damage of centrifugal treating, improve the quality of sperm sperm.The utility model can be used as the instrument of auxiliary procreation technology.
Description of drawings
Fig. 1 is the utility model structural representation.
Embodiment
With reference to Fig. 1; The preferred micro fluidic device of a kind of sperm; Comprise slide glass 8 and the substrate 9 that supports slide glass 8, be provided with damping fluid addition pool 4, pattern detection pond 2 and sample collection pond 5 at slide glass 8,2 in damping fluid addition pool 4 and pattern detection pond are provided with damping fluid connecting passage 6; 2 inside, pattern detection pond are provided with sample effect pond 1, and 5 in pattern detection pond 2 and sample collection pond are provided with sample collection passage 7.Pattern detection pond 2 and 1 in sample effect pond are provided with a perhaps screening passage 3 more than, have at least a bar grizzl(e)y gating road 3 to be located at the side that pattern detection pond 2 is connected with sample collection pond 5.Slide glass 8 is processed by the YSR 3286 material, and substrate 9 is processed by glass or quartzy slide or YSR 3286 material, and slide glass 8 is fixed through the sealing-in mode with substrate 9.Certainly slide glass 8 and substrate 9 also can be processed and not influenced the protection domain of the utility model by other macromolecule polymer material.
With reference to Fig. 1; The preferred embodiment of the preferred micro fluidic device of a kind of sperm of the utility model, circular sample effect pond 1, cyclic pattern detection pond 2, screening passage 3, damping fluid addition pool 4, sample collection pond 5, damping fluid connecting passage 6 and the sample collection passage 7 of etching on the slide glass surface.The diameter in sample effect pond 1 is 5 millimeters; The width in cyclic pattern detection pond 2 is 4 millimeters; 7 bar grizzl(e)y gating roads 3 are arranged between sample effect pond 1 and the pattern detection pond 2; The width of screening passage 3 is 0.02 millimeter, and the degree of depth is 0.02 millimeter, and the degree of depth of damping fluid connecting passage 6 and sample collection passage 7 also is 0.02 millimeter.
Diameter, width or the degree of depth of sample effect pond 1, pattern detection pond 2, screening passage 3, damping fluid addition pool 4, sample collection pond 5, damping fluid connecting passage 6 and sample collection passage 7 can not influence the protection domain of the utility model for other numerical value yet in the utility model.
Certainly, sample effect pond 1 and pattern detection pond 2 also can be rectangle or other shapes and do not influence the protection domain of utility model in the preferred micro fluidic device of the utility model sperm.
When the utility model uses; Wash described screening passage 3, damping fluid connecting passage 6 and sample collection passage 7; Exhaust the liquid in said damping fluid addition pool 4, pattern detection pond 2, sample effect pond 1 and the sample collection pond 5, be full of above-mentioned passage with damping fluid then, in sample effect pond 1, add the cervical mucus that is in women of child-bearing age's onset of ovulation; On sample effect pond 1, add seminal fluid then, the preferred micro fluidic device of sperm was put into 37 ℃ of carbonic acid gas incubators 5 minutes.Because the interaction of sperm and cervical mucus utilizes the random of sperm to move about under physiological status, the sperm that vigor is good can be swum in the pattern detection pond 2 through 7 bar grizzl(e)y gating roads 3.In pattern detection pond 2, the sperm that optimizes is carried out the vigor analysis through the area of computer aided sperm analysis system.After 20 minutes; The sperm that is arranged in pattern detection pond 2 in damping fluid addition pool 4, adds certain damping fluid, owing under the effect of hydrostaticpressure, can be taken to sample collection pond 5 by flow; Draw the collection liquid in the sample collection pond 5, accomplish the screening and the collection of the sperm of high vigor like this.
Certainly; The utility model is created and is not limited to above-mentioned embodiment; Those of ordinary skill in the art also can make equivalent variations or replacement under the prerequisite of the utility model spirit, modification that these are equal to or replacement all are included in the application's claim institute restricted portion.
Claims (3)
1. preferred micro fluidic device of sperm; It is characterized in that: the substrate (9) that comprises slide glass (8) and support slide glass (8); Be provided with damping fluid addition pool (4), pattern detection pond (2) and sample collection pond (5) at slide glass (8); Be provided with damping fluid connecting passage (6) between said damping fluid addition pool (4) and pattern detection pond (2); Be provided with sample effect pond (1) in the said pattern detection pond (2); Be provided with one between said pattern detection pond (2) and sample effect pond (1) and perhaps screen passage (3) more than one, have at least a bar grizzl(e)y gating road (3) to be located at the side that pattern detection pond (2) is connected with sample collection pond (5), be provided with sample collection passage (7) between said pattern detection pond (2) and sample collection pond (5).
2. the preferred micro fluidic device of sperm according to claim 1 is characterized in that: said slide glass (8) is fixed through sealing-in with substrate (9).
3. the preferred micro fluidic device of sperm according to claim 1 is characterized in that: said slide glass (8) is processed by the YSR 3286 material, and substrate (9) is processed by glass or quartzy slide or YSR 3286 material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203698572U CN202297576U (en) | 2011-09-27 | 2011-09-27 | Microfluidic device for sperm optimization |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203698572U CN202297576U (en) | 2011-09-27 | 2011-09-27 | Microfluidic device for sperm optimization |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202297576U true CN202297576U (en) | 2012-07-04 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011203698572U Expired - Fee Related CN202297576U (en) | 2011-09-27 | 2011-09-27 | Microfluidic device for sperm optimization |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202297576U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103969193A (en) * | 2013-01-28 | 2014-08-06 | 郑兆珉 | sperm quality detection device |
| CN113528310A (en) * | 2021-06-16 | 2021-10-22 | 复旦大学 | Bionic micro-fluidic chip for simulating cervical microenvironment and preparation method thereof |
| CN114216901A (en) * | 2021-11-22 | 2022-03-22 | 江苏大学 | Sperm activity detection system and method based on micro-fluidic chip composite smart phone |
-
2011
- 2011-09-27 CN CN2011203698572U patent/CN202297576U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103969193A (en) * | 2013-01-28 | 2014-08-06 | 郑兆珉 | sperm quality detection device |
| CN113528310A (en) * | 2021-06-16 | 2021-10-22 | 复旦大学 | Bionic micro-fluidic chip for simulating cervical microenvironment and preparation method thereof |
| CN114216901A (en) * | 2021-11-22 | 2022-03-22 | 江苏大学 | Sperm activity detection system and method based on micro-fluidic chip composite smart phone |
| CN114216901B (en) * | 2021-11-22 | 2024-03-19 | 江苏大学 | Sperm activity detection system and method based on microfluidic chip composite smart phone |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120704 Termination date: 20120927 |