CN202279825U - Simulated experiment device for cell low-pressure hypoxia condition - Google Patents
Simulated experiment device for cell low-pressure hypoxia condition Download PDFInfo
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- CN202279825U CN202279825U CN 201120401522 CN201120401522U CN202279825U CN 202279825 U CN202279825 U CN 202279825U CN 201120401522 CN201120401522 CN 201120401522 CN 201120401522 U CN201120401522 U CN 201120401522U CN 202279825 U CN202279825 U CN 202279825U
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- cell
- sealing cabinet
- simulated experiment
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- lamina membranacea
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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Abstract
The utility model provides a simulated experiment device for a cell low-pressure hypoxia condition. The simulated experiment device comprises a sealing cabinet body with a sealing door, wherein an incubating device is arranged in the sealing cabinet body; a nutrient solution supply device is connected with a multilayer cell membrane plate rack through a pipeline; an ozone sterilizer and an ultraviolet sterilizing lamp are arranged at the inner top of the sealing cabinet body; measurement and control devices for temperature, humidity, pressure, and concentrations of nitrogen and carbon dioxide are arranged at the upper end of the sealing cabinet body; nitrogen and carbon dioxide sources with flow control valve, an air conditioning circulating pump and a vacuumizing pump are connected with the exterior of the sealing cabinet body; and all the measurement and control sensors of the device, the flow control valve and control valves of pumps are all connected with a computer, so as to perform data transmission, operate according to a pre-compiled program and perform a simulated experiment of a low-pressure hypoxia condition. The simulated experiment device for the cell low-pressure hypoxia condition has the beneficial effects that an experimental animal is directly replaced by a cell, the experimental cost and time are saved, the experimental efficiency is increased, a new automatic device is supplied for the related pathological research, and the simulated experiment device can be widely applied to the simulated experiment of the cell low-pressure hypoxia condition for researching the pathology of a respiratory and circulatory system.
Description
Technical field:
The utility model provides a kind of cell hypobaric hypoxia environment analogue experiment installation.
Background technology:
Pathological study in the past more under the hypobaric hypoxia condition uses animal to carry out needed simulated experiment.Device that needs and material take up room bigger, and required device is numerous and diverse valuable, and control accuracy is difficult to reach requirement.The hypobaric hypoxia simulated experiment of adopting the cell replacement animal to carry out along with scientific development is an emerging experimental technique in the pathological study under the current medical science mesolow hypoxia condition; Research and develop a hypobaric hypoxia environment analogue experiment installation for this reason and just become current urgent needs.
The utility model content:
For addressing the above problem; The purpose of the utility model provides a kind of cell hypobaric hypoxia environment analogue experiment installation; Can accurately control experiment parameter for relevant scientific research provides a kind of, carry out the analogue experiment installation of cell hypobaric hypoxia environment safely, efficiently.This device has direct express cell response effect, and the experimental result scientific and precise is without laboratory animal, save experiment fees and time, improved advantage such as conventional efficient greatly.Cell hypobaric hypoxia environment analogue experiment installation be under the current medical science mesolow hypoxia condition in the pathological study emerging experimental technique new automated installation is provided, can in relevant medical scientific, be widely used.
For realizing above-mentioned purpose; The technical scheme of the utility model is a kind of cell hypobaric hypoxia environment analogue experiment installation, and said equipment comprises that having sealing cabinet integral body is made by the partiting thermal insulation performance materials, and sealing cabinet and hermatic door inner surface material are made by stainless steel plate; Corrosion-resistant and be convenient to the cleaning; Be provided with movable hinge between sealing cabinet and the hermatic door and connect, joint strip is installed around the hermatic door, guarantee good seal.The cabinet inner bottom part is provided with incubator; Incubator top is provided with multi-layer cellular lamina membranacea frame; Multi-layer cellular lamina membranacea frame is made by the metallic substance of tool heat conductivility, and the cell growing environment that guarantees control is at 37 degrees centigrade, and every confluent monolayer cells lamina membranacea frame bottom all is provided with the nutritive medium feeding mechanism and connects through the nutritive medium supply line; The cell lamina membranacea directly is placed on the cytolemma grillage, and the cytolemma of cell lamina membranacea directly soaks in the nutritive medium feeding mechanism; Sealing cabinet inner top is provided with ozonateur and ultraviolet sterilization lamp; The cabinet upper end is provided with temperature detecting controller, humidity controller, pressure testing and controlling device, nitrogen gas concn controller and gas concentration lwevel controller and all is connected to computingmachine, and the nitrogen source of the gas of outside connecting band nitrogen flow valve also is connected with computingmachine with vacuum pumping pump with the carbon dioxide air source and the air-conditioning recycle pump of band carbon-dioxide flow control valve.
The beneficial effect of the utility model is: a kind of cell hypobaric hypoxia environment simulation experiment device is provided; Adopt the simulated experiment of cell hypobaric hypoxia environment to substitute the hypobaric hypoxia environment simulated experiment of carrying out with animal; Equipment used is littler than the used experimental installation of experimentation on animals because the use cell experimentizes; Can better control experimental precision; Better various interfering factorss of shielding are investigated cell more accurately because cause-effect relationshiies such as the infringement that hypobaric hypoxia causes and treat-ment, and the starting material that consumed and all savings greatly of time; It has direct express cell response effect, and the experimental result scientific and precise does not use laboratory animal, saves experiment fees and time, improved conventional efficient greatly; For emerging experimental technique in the pathological study under the current medical science mesolow hypoxia condition provides new automatic equipment, can in relevant medical scientific, be widely used.
Description of drawings:
Accompanying drawing 1 is the synoptic diagram of the utility model structure
1, hermatic door
2, sealing cabinet
3, temperature detecting controller
4, humidity controller
5, pressure testing and controlling device
6, nitrogen gas concn controller
7, gas concentration lwevel controller
8, carbon-dioxide flow control valve
9, nitrogen flow valve
10, carbon dioxide air source
11, nitrogen source of the gas
12, air-conditioning recycle pump
13, vacuum pumping pump
14, cytolemma grillage
15, nutritive medium feeding mechanism
16, ozonateur
17, ultraviolet sterilization lamp
18, computingmachine
19, stamping machine
20, incubator
21, nutritive medium supply line
Embodiment:
In conjunction with accompanying drawing 1 and embodiment the utility model is explained.
The utility model provides a kind of cell hypobaric hypoxia environment analogue experiment installation; Said equipment comprises that sealing cabinet (2) integral body is made by the partiting thermal insulation performance materials; Sealing cabinet (2) and hermatic door (1) material inside are that stainless steel plate is made; Be provided with movable hinge between sealing cabinet (2) and the hermatic door (1) and connect, hermatic door (1) is equipped with joint strip all around, guarantees temperature, humidity and pressure and the gas flow conditional stability of sealed cabinet; Be provided with incubator 20 in the cabinet; Accurately control seals the cabinet internal temperature; Incubator 20 tops are provided with multi-layer cellular lamina membranacea frame 14, and multi-layer cellular lamina membranacea frame 14 is made by the metallic substance of tool heat conductivility, and nutritive medium feeding mechanism 15 is connected to every confluent monolayer cells lamina membranacea frame 14 by nutritive medium supply line 21; Every confluent monolayer cells lamina membranacea frame 14 bottoms all are provided with nutritive medium feeding mechanism 15 and connect through nutritive medium supply line 21; The cell lamina membranacea directly is placed on the cytolemma grillage 14, and the cytolemma of cell lamina membranacea directly soaks in nutritive medium feeding mechanism 15, and cell can obtain satisfied existence and growing environment.Cytolemma grillage 14 can be placed a large amount of cell lamina membranaceas simultaneously, and convenient carrying out simultaneously organized cell experiments more, improved conventional efficient; The cabinet inner top is provided with ozonateur 16 and ultraviolet sterilization lamp 17, is sterilization in order to carry out cabinet inside, reaches the operation that under aseptic condition, experimentizes; The cabinet upper end is provided with temperature detecting controller 3, humidity controller 4, pressure testing and controlling device 5, nitrogen gas concn controller 6 and gas concentration lwevel controller 7 and all is connected to computingmachine 18; The nitrogen source of the gas 11 of outside connecting band nitrogen flow valve 9 also links to each other with computingmachine 18 with vacuum pumping pump 13 with the carbon dioxide air source 10 and the air-conditioning recycle pump 12 of band carbon-dioxide flow control valve 8; All the observing and controlling transmitters of equipment and the valve of flowrate control valve and pump all are connected with computingmachine 18; Supplying nitrogen or dioxide gas reduce sealing cabinet 2 inner oxygen contents to computingmachine 18 successively in sealing cabinet 2 according to the time variable control nitrogen flow valve of finishing in advance 9 and carbon-dioxide flow control valve 8; Starting vacuum pumping pump 13 makes sealing cabinet 2 internal pressures arrive requirement of experiment; Control air-conditioning recycle pump 12 flows sealing cabinet 2 internal gas, and experimental situation is even; Thereby carry out the cell hypobaric hypoxia environment simulated experiment of setting-up time length; Experimental data can be kept in the computingmachine 18 or print through stamping machine 19.
Embodiment:
When using the utility model cell hypobaric hypoxia environment analogue experiment installation; At first energized is also opened the ozonateur 16 and ultraviolet sterilization lamp 17 that the cabinet inner top is provided with respectively; To carry out thorough disinfection to whole cell hypobaric hypoxia environment simulation experiment device inside, reach the operation that under aseptic condition, experimentizes; There is the cell lamina membranacea of experimental cell to be placed on the cytolemma grillage 14 in following a plurality of inoculations of aseptic condition; Be provided with and start incubator 20 and nutritive medium feeding mechanism 15; Make whole metal cell lamina membranacea frame 14 accurate temperature controllings at 37 degrees centigrade; And guarantee that the cell nutrient solution in the nutritive medium feeding mechanism 15 carried on the cell shuttering of competent cell culture fluid in the cytolemma grillage 14 of each layer by pipeline; Closed hermatic door 1; Sealing cabinet 2 complete closed are finished computingmachine 18 programs, make whole cell hypobaric hypoxia environment simulation experiment device under suitable humiture and nutritive medium supply condition; Experimental cell can obtain satisfied existence and growing environment; In this normal habitat, by the time variable control nitrogen flow valve of finishing in advance 9 and carbon-dioxide flow control valve 8 supplying nitrogen successively or the dioxide gas in sealing cabinet 2 sealing cabinet 2 inner oxygen contents are reduced to by computingmachine 18 and requirement are set, start vacuum pumping pump and make sealing cabinet 2 internal pressures arrive requirement of experiment, control air-conditioning recycle pump evenly flows sealing cabinet 2 internal gas and in time controls internal temperature and humidity environment; Thereby carry out the cell hypobaric hypoxia environment simulated experiment of setting-up time length; With closing carbon dioxide and nitrogen valve, under the gnotobasis condition, open and seal cabinet 2 takes out experimental cell from cytolemma grillage 14 cell lamina membranacea and carry out specialty analysis and processing after experiment is accomplished.
Claims (3)
1. cell hypobaric hypoxia environment analogue experiment installation; It is characterized in that: said device comprises the sealing cabinet (2) that has hermatic door (1); The cabinet inner bottom part is provided with incubator (20); Incubator (20) top is provided with multi-layer cellular lamina membranacea frame (14); Sealing cabinet (2) inner top is provided with ozonateur (16) and ultraviolet sterilization lamp (17); The cabinet upper end is provided with temperature detecting controller (3), humidity controller (4), pressure testing and controlling device (5), nitrogen gas concn controller (6) and gas concentration lwevel controller (7) and all is connected to computingmachine (18), and the nitrogen source of the gas (11) of outside connecting band nitrogen flow valve (9) also is connected with computingmachine (18) with vacuum pumping pump (13) with the carbon dioxide air source (10) and the air-conditioning recycle pump (12) of band carbon-dioxide flow control valve (8).
2. a kind of cell hypobaric hypoxia environment simulation experiment device according to claim 1; It is characterized in that: described sealing cabinet (2) is whole to be made by the partiting thermal insulation performance materials; Sealing cabinet (2) and hermatic door (1) material inside are that stainless steel plate is made; Be provided with movable hinge between sealing cabinet (2) and the hermatic door (1) and connect, hermatic door (1) is equipped with joint strip all around.
3. a kind of cell hypobaric hypoxia environment simulation experiment device according to claim 1; It is characterized in that: described multi-layer cellular lamina membranacea frame (14) is made by the metallic substance of tool heat conductivility; Every confluent monolayer cells lamina membranacea frame (14) bottom all is provided with nutritive medium feeding mechanism (15) and connects through nutritive medium supply line (21); The cell lamina membranacea directly is placed on the cytolemma grillage (14), and the cytolemma of cell lamina membranacea directly soaks in nutritive medium feeding mechanism (15).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120401522 CN202279825U (en) | 2011-10-20 | 2011-10-20 | Simulated experiment device for cell low-pressure hypoxia condition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120401522 CN202279825U (en) | 2011-10-20 | 2011-10-20 | Simulated experiment device for cell low-pressure hypoxia condition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202279825U true CN202279825U (en) | 2012-06-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120401522 Expired - Fee Related CN202279825U (en) | 2011-10-20 | 2011-10-20 | Simulated experiment device for cell low-pressure hypoxia condition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202279825U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102352313A (en) * | 2011-10-20 | 2012-02-15 | 天津开发区合普工贸有限公司 | Cellular low-pressure low-oxygen environment simulated experiment system |
| CN102719355A (en) * | 2012-07-19 | 2012-10-10 | 苏州大学 | Cell vacuum absorption fixing device |
| CN104799967A (en) * | 2015-04-29 | 2015-07-29 | 山东大学 | Integrated hypoxia experiment simulation device and method of mammals |
| CN116769595A (en) * | 2023-06-19 | 2023-09-19 | 中国人民解放军空军军医大学 | Low-pressure low-oxygen cell simulator |
-
2011
- 2011-10-20 CN CN 201120401522 patent/CN202279825U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102352313A (en) * | 2011-10-20 | 2012-02-15 | 天津开发区合普工贸有限公司 | Cellular low-pressure low-oxygen environment simulated experiment system |
| CN102719355A (en) * | 2012-07-19 | 2012-10-10 | 苏州大学 | Cell vacuum absorption fixing device |
| CN104799967A (en) * | 2015-04-29 | 2015-07-29 | 山东大学 | Integrated hypoxia experiment simulation device and method of mammals |
| CN104799967B (en) * | 2015-04-29 | 2017-03-15 | 山东大学 | Integral type mammal anoxic experimental simulation device and method |
| CN116769595A (en) * | 2023-06-19 | 2023-09-19 | 中国人民解放军空军军医大学 | Low-pressure low-oxygen cell simulator |
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Granted publication date: 20120620 Termination date: 20171020 |
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| CF01 | Termination of patent right due to non-payment of annual fee |