CN202099277U - 模拟瘤胃体外发酵产气装置 - Google Patents

模拟瘤胃体外发酵产气装置 Download PDF

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CN202099277U
CN202099277U CN 201120190375 CN201120190375U CN202099277U CN 202099277 U CN202099277 U CN 202099277U CN 201120190375 CN201120190375 CN 201120190375 CN 201120190375 U CN201120190375 U CN 201120190375U CN 202099277 U CN202099277 U CN 202099277U
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syringe
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fermentation
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刘立成
宋伟红
刘光前
赵福忠
卫喜明
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/04—Bioreactors or fermenters specially adapted for specific uses for producing gas, e.g. biogas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles

Abstract

模拟瘤胃体外发酵产气装置属于动物生理研究器械；在注射器下端针头座上密封安装医用三通阀，塑料软管的上端与医用三通阀密封连接，在塑料软管的下侧外部上密封配装橡胶塞，橡胶塞密封插装在产气发酵培养瓶的瓶口内，医用三通阀和塑料软管将注射器与产气发酵培养瓶相互连通；本装置结构简单，造价低廉，模拟瘤胃体外产气量读数准确，体外产气气体样品采集方便，有利于动物科学科研质量和水平的提高。

Description

模拟瘤胃体外发酵产气装置
技术领域
本实用新型属于动物生理研究器械，主要涉及一种反刍动物瘤胃发酵环境的模拟体外培养装置。
背景技术
目前，简易的人工瘤胃体外培养装置主要是将注射器作为发酵罐，将培养液和发酵底物放到注射器里进行模拟瘤胃内环境的试验研究。但是，由于注射器很小，且培养条件需要不断震荡，培养液、培养底物、发酵产物及产生的气体都集中在注射器中，在震荡的条件下注射器的读数不稳定，气体采集不方便，容易造成试验误差。
发明内容
本实用新型的目的就是针对上述现有技术存在的问题，设计提供一种新结构的模拟瘤胃体外发酵产气装置，达到准确完成体外产气量的读取、方便体外产气样品采集的目的。
本实用新型的基本设计是，在注射器下端针头座上密封安装医用三通阀，塑料软管的上端与医用三通阀密封连接，在塑料软管的下侧外部上密封配装橡胶塞，橡胶塞密封插装在产气发酵培养瓶的瓶口内，医用三通阀和塑料软管将注射器与产气发酵培养瓶相互连通，至此构成模拟瘤胃体外发酵产气装置。
本实用新型结构简单，造价低廉，模拟瘤胃体外产气量读数准确，体外产气气体样品采集简易方便，有利于动物科学研究质量和水平的提高。
附图说明
附图是模拟瘤胃体外发酵产气装置总体结构示意图。
图中件号说明：
1、注射器、2、医用三通阀、3、塑料软管、4、橡胶塞、5、产气发酵培养瓶。
具体实施方式
下面结合附图对本实用新型最佳实施方案进行详细描述。一种模拟瘤胃体外发酵产气装置，在注射器1下端针头座上密封安装医用三通阀2，塑料软管3的上端与医用三通阀2密封连接，在塑料软管3的下侧外部上密封配装橡胶塞4，橡胶塞4密封插装在产气发酵培养瓶5的瓶口内，医用三通阀2和塑料软管3将注射器1与产气发酵培养瓶5相互连通。
体外培养使用时，首先向产气发酵培养瓶5内加入培养底物和培养液后，将产气发酵培养瓶5放在恒温水浴摇床上震荡，将注射器1固定在一个支架上，利用医用三通阀2将产气发酵培养瓶5与注射器1连通即可。在温度适宜的情况下，产气发酵培养瓶5中微生物和培养底物发酵产生大量的气体，气体通过塑料软管3进入注射器1内，当产气量达到注射器1量程或某一特定的刻度时，记录产气量，并利用医用三通阀2排放注射器1内的气体；如果需要进行气体成分分析，可以用气体采样袋通过医用三通阀2的排气孔进行气体收集，在排放气体和收集气体的过程中不需要打开产气发酵培养瓶5，保证其一直处于厌氧发酵的状态。

Claims (1)

1.一种模拟瘤胃体外发酵产气装置，其特征在于在注射器(1)下端针头座上密封安装医用三通阀(2)，塑料软管(3)的上端与医用三通阀(2)密封连接，在塑料软管(3)的下侧外部上密封配装橡胶塞(4)，橡胶塞(4)密封插装在产气发酵培养瓶(5)的瓶口内，医用三通阀(2)和塑料软管(3)将注射器(1)与产气发酵培养瓶(5)相互连通。
CN 201120190375 2011-06-08 2011-06-08 模拟瘤胃体外发酵产气装置 Expired - Fee Related CN202099277U (zh)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102590453A (zh) * 2012-02-17 2012-07-18 徐�明 一种用于体外瘤胃产气法的磷酸缓冲液
CN102798557A (zh) * 2012-08-10 2012-11-28 中国科学院亚热带农业生态研究所 用于物质养分生物利用实时在线检测的方法及反应器
CN103698165A (zh) * 2014-01-06 2014-04-02 中国农业大学 一种厌氧发酵体系中气体样品和/或液体样品的收集方法
CN109207354A (zh) * 2018-11-19 2019-01-15 农业部规划设计研究院 一种微生物发酵产气潜力测试装置
CN110499242A (zh) * 2019-09-24 2019-11-26 扬州大学 一种瘤胃上皮组织体外培养的模拟装置及其模拟方法
CN114350475A (zh) * 2022-01-07 2022-04-15 河北农业大学 一种真空袋式瘤胃体外发酵装置及其运行方法

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102590453A (zh) * 2012-02-17 2012-07-18 徐�明 一种用于体外瘤胃产气法的磷酸缓冲液
CN102590453B (zh) * 2012-02-17 2015-01-21 徐�明 一种用于体外瘤胃产气法的磷酸缓冲液
CN102798557A (zh) * 2012-08-10 2012-11-28 中国科学院亚热带农业生态研究所 用于物质养分生物利用实时在线检测的方法及反应器
CN103698165A (zh) * 2014-01-06 2014-04-02 中国农业大学 一种厌氧发酵体系中气体样品和/或液体样品的收集方法
CN109207354A (zh) * 2018-11-19 2019-01-15 农业部规划设计研究院 一种微生物发酵产气潜力测试装置
CN109207354B (zh) * 2018-11-19 2023-09-05 农业部规划设计研究院 一种微生物发酵产气潜力测试装置
CN110499242A (zh) * 2019-09-24 2019-11-26 扬州大学 一种瘤胃上皮组织体外培养的模拟装置及其模拟方法
CN114350475A (zh) * 2022-01-07 2022-04-15 河北农业大学 一种真空袋式瘤胃体外发酵装置及其运行方法
CN114350475B (zh) * 2022-01-07 2023-07-18 河北农业大学 一种真空袋式瘤胃体外发酵装置及其运行方法

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