CN202152343U - 人工瘤胃体外发酵产气装置 - Google Patents

人工瘤胃体外发酵产气装置 Download PDF

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CN202152343U
CN202152343U CN 201120296696 CN201120296696U CN202152343U CN 202152343 U CN202152343 U CN 202152343U CN 201120296696 CN201120296696 CN 201120296696 CN 201120296696 U CN201120296696 U CN 201120296696U CN 202152343 U CN202152343 U CN 202152343U
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gas
syringe
soft
rubber plug
vitro
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曲永利
刘立成
李伟
宋伟红
苗树君
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Heilongjiang Bayi Agricultural University
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Heilongjiang Bayi Agricultural University
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    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/04—Bioreactors or fermenters specially adapted for specific uses for producing gas, e.g. biogas
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M23/02—Form or structure of the vessel
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure

Abstract

人工瘤胃体外发酵产气装置属于动物生理研究器械；在注射器下端上安装三通阀，输气软管的上端与三通阀连接，在输气软管下侧外部上配装橡胶塞，橡胶塞装在产气发酵培养瓶的瓶口内，输气软管和三通阀将产气发酵培养瓶与注射器相互连通，导气软管一端部插装在橡胶塞上，其另一端与气体压力表连接，导气软管将产气发酵培养瓶与气体压力表相互连通；本装置结构简单，造价低廉，人工方法模拟瘤胃体外产气量准确，体外产气气体样品采集简易方便，有利于动物科研质量的提高。

Description

人工瘤胃体外发酵产气装置
技术领域
本实用新型属于动物生理研究器械，主要涉及一种反刍动物瘤胃发酵环境的模拟体外培养装置。
背景技术
目前，简易的人工瘤胃体外培养装置主要是将注射器作为发酵罐，将培养液和发酵底物放到注射器里进行模拟瘤胃内环境的试验研究。但是，由于注射器很小，且培养条件需要不断震荡，培养液、培养底物、发酵产物及产生的气体都集中在注射器中，在震荡的条件下注射器的读数不稳定，气体采集不方便，容易造成试验误差。
发明内容
本实用新型的目的就是针对上述现有技术存在的问题，设计提供一种新结构的人工瘤胃体外发酵产气装置，达到准确完成体外产气量的读取、方便体外产气样品采集的目的。
本实用新型的基本设计是，在注射器下端上密封连接安装三通阀，输气软管的上端与三通阀密封连接，在输气软管下侧外部上密封配装橡胶塞，橡胶塞密封塞装在产气发酵培养瓶的瓶口内，输气软管和三通阀将产气发酵培养瓶与注射器相互连通；导气软管一端部密封插装在橡胶塞上，其另一端与气体压力表连接，导气软管将产气发酵培养瓶与气体压力表相互连通，至此构成人工瘤胃体外发酵产气装置。
本实用新型结构简单，造价低廉，模拟瘤胃体外产气量读数准确，体外产气气体样品采集简易方便，有利于动物科学研究质量和水平的提高。
附图说明
附图是人工瘤胃体外发酵产气装置总体结构示意图。
图中件号说明：
1、注射器、2、三通阀、3、输气软管、4、橡胶塞、5、产气发酵培养瓶、6、气体压力表、7、导气软管。
具体实施方式
下面结合附图对本实用新型最佳实施方案进行详细描述。一种人工瘤胃体外发酵产气装置，在注射器1下端上密封连接安装三通阀2，输气软管3的上端与三通阀2密封连接，在输气软管3下侧外部上密封配装橡胶塞4，橡胶塞4密封塞装在产气发酵培养瓶5的瓶口内，输气软管3和三通阀2将产气发酵培养瓶5与注射器1相互连通；导气软管7一端部密封插装在橡胶塞4上，其另一端与气体压力表6连接，导气软管7将产气发酵培养瓶5与气体压力表6相互连通。
人工模拟体外培养使用时，首先向产气发酵培养瓶5内加入培养底物和培养液后，将产气发酵培养瓶5放在恒温水浴摇床上震荡，将注射器1固定在一个支架上，利用三通阀2将产气发酵培养瓶5与注射器1连通即可。在温度适宜的情况下，产气发酵培养瓶5中微生物和培养底物发酵产生大量的气体，气体通过输气软管3和三通阀2进入注射器1内。当达到设定的测量时间时，利用注射器1使气体压力表6的指针指向1个大气压刻线位置后，即可读取记录注射器1内的产气量。当产气量达到注射器1量程或某一特定的刻度时，记录产气量，并利用三通阀2将注射器1内的气体排出；如果需要进行气体成分分析，可以用气体采样袋通过三通阀2的排气孔进行气体收集，在排放气体和收集气的过程中不需要打开产气发酵培养瓶5，保证其一直处于厌氧发酵的状态。

Claims (1)

1.一种人工瘤胃体外发酵产气装置，其特征在于在注射器(1)下端上密封连接安装三通阀(2)，输气软管(3)的上端与三通阀(2)密封连接，在输气软管(3)下侧外部上密封配装橡胶塞(4)，橡胶塞(4)密封塞装在产气发酵培养瓶(5)的瓶口内，输气软管(3)和三通阀(2)将产气发酵培养瓶(5)与注射器(1)相互连通；导气软管(7)一端部密封插装在橡胶塞(4)上，其另一端与气体压力表(6)连接，导气软管(7)将产气发酵培养瓶(5)与气体压力表(6)相互连通。
CN 201120296696 2011-08-16 2011-08-16 人工瘤胃体外发酵产气装置 Expired - Fee Related CN202152343U (zh)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102798557A (zh) * 2012-08-10 2012-11-28 中国科学院亚热带农业生态研究所 用于物质养分生物利用实时在线检测的方法及反应器
CN109207354A (zh) * 2018-11-19 2019-01-15 农业部规划设计研究院 一种微生物发酵产气潜力测试装置
CN110499242A (zh) * 2019-09-24 2019-11-26 扬州大学 一种瘤胃上皮组织体外培养的模拟装置及其模拟方法

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102798557A (zh) * 2012-08-10 2012-11-28 中国科学院亚热带农业生态研究所 用于物质养分生物利用实时在线检测的方法及反应器
CN109207354A (zh) * 2018-11-19 2019-01-15 农业部规划设计研究院 一种微生物发酵产气潜力测试装置
CN109207354B (zh) * 2018-11-19 2023-09-05 农业部规划设计研究院 一种微生物发酵产气潜力测试装置
CN110499242A (zh) * 2019-09-24 2019-11-26 扬州大学 一种瘤胃上皮组织体外培养的模拟装置及其模拟方法

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