The utility model content
The problem that the utility model solves provides a kind of perfusion structure of mesenterium microcirculation finder, its objective is the Intraabdominal environmental baseline of simulated animal, improves the little observing effect that follows of mesenterium.
To achieve these goals, the technical scheme taked of the utility model is:
The perfusion structure of this mesenterium microcirculation finder provided by the utility model, described mesenterium microcirculation finder is used for that checking matter is carried out microcirculation to be observed, described checking matter is the mesenterium of animal, described finder is provided with the microcirculation pond, in described microcirculation pond, be provided with sponge, described checking matter is placed on the described sponge, described finder is provided with the perfusion structure, described perfusion structure is provided with the infusion bag above described microcirculation pond, and is connected in the described microcirculation pond by woven hose; Described woven hose forms the perfusion pendular ring of annular in the position of described checking matter, the mesenterium articulating of its size and described checking matter is near; Described perfusion pendular ring is provided with the aperture that a plurality of sepages are used in different positions.
Center in described microcirculation pond is provided with columniform microcirculation pond inner convex platform, and described sponge is the structure of hollow, and described circulatory pool inner convex platform is positioned at wherein; Around multi-turn, its latter end is described perfusion pendular ring to described woven hose on the inner convex platform cylindrical of described microcirculation pond.
Described perfusion structure is established the drainage thread that many plying, cotton materials are made, and the one end is located at described checking matter place; The other end is located at below, described microcirculation pond, and this end is placed in the liquid collecting jar.
The utility model adopts technique scheme, the perfusion structural design of mesenterium microcirculation finder is ingenious rationally, use is easy to operate, consistent, the microcirculation observing effect is good, solve the technical barrier of external mesenterium microcirculation simulation intraperitoneal environment better, be the comparatively ideal finder of mesenterium microcirculation.Adopt the warm heating of thermostatical circulating water, that this method has is simple, stable, temperature is adjustable, the advantage of uniform of heating, and has solved the insulation problem of microcirculation finder better.Adopt transfusion drip irrigation drainage perfusate to add preservative film and post method, this method is simple, stable, does not influence observing environment, and insulation arranged, preserve moisture, perfusion, fixation.
Description of drawings
Below expressed content of each width of cloth accompanying drawing of this instructions and the mark among the figure are made brief description:
Fig. 1 is a structural representation of the present utility model;
Fig. 2 is insulation of the present utility model, the structural representation of preserving moisture;
Fig. 3 is the synoptic diagram of the perfusion structure in the utility model.
Be labeled as among the figure:
1, microcirculation pond, 2, thermostat water bath, 3, sponge, 4, preservative film, 5, proofed sleeve, 6, checking matter, 7, microscope, 8, thermometer, 9, operating table, 10, perfusion structure, 11, infusion bag, 12, woven hose, 13, perfusion pendular ring, 14, drainage thread, 15, the liquid collecting jar, 16, microcirculation pond inner convex platform.
Embodiment
Contrast accompanying drawing below, by description to embodiment, to effect and principle of work, the manufacturing process of the mutual alignment between the shape of embodiment of the present utility model such as related each member, structure, the each several part and annexation, each several part and manipulate method etc., be described in further detail, inventive concept of the present utility model, technical scheme had more complete, accurate and deep understanding to help those skilled in the art.
As Fig. 1, Fig. 2 and the expressed structure of the present utility model of Fig. 3, be a kind of perfusion structure of mesenterium microcirculation finder, be used for that checking matter 6 is carried out microcirculation and observe, described checking matter 6 is the mesenterium of animal.Described finder is provided with microcirculation pond 1, is provided with sponge 3 in described microcirculation pond 1, and described checking matter 6 is placed on the described sponge 3.
In order to solve the problem that exists at the described present known technology of this instructions background technology part and to overcome its defective, realize improving the goal of the invention of the little observing effect that follows of mesenterium, the technical scheme that the utility model is taked is:
As Fig. 1, Fig. 2 and shown in Figure 3, the perfusion structure of this mesenterium microcirculation finder provided by the utility model is provided with microcirculation pond 1 and thermostat water bath 2, in described microcirculation pond 1, be provided with sponge 3, described checking matter 6 is placed on the described sponge 3, and described microcirculation pond 1 is communicated with described thermostat water bath 2 by two proofed sleeves 5.
Described finder is provided with perfusion structure 10, and described perfusion structure 10 is provided with the infusion bag 11 above described microcirculation pond 1, and is connected in the described microcirculation pond 1 by woven hose 12; Described woven hose 12 forms the perfusion pendular ring 13 of annular in the position of described checking matter 6, the mesenterium articulating of its size and described checking matter 6 is near; Described perfusion pendular ring 13 is provided with the aperture that a plurality of sepages are used in different positions.
In the infusion bag 11 liquid, so that experimental situation is similar as far as possible to the environment in the animal body near animal body body fluid.
Adopt technique scheme, the perfusion structural design of mesenterium microcirculation finder is ingenious rationally, use is easy to operate, consistent, the microcirculation observing effect is good, solve the technical barrier of external mesenterium microcirculation simulation intraperitoneal environment better, be the comparatively ideal finder of mesenterium microcirculation.Avoided in the prior art by hand drop, constant flow pump to irritate the drip perfusate, troublesome poeration, instability, device complexity, influence observing environment, the perfusate location is forbidden, can not be to defectives such as the direct perfusions of mesenterium.Adopt the warm heating of thermostatical circulating water, that this method has is simple, stable, temperature is adjustable, the advantage of uniform of heating, and has solved the insulation problem of microcirculation finder better.
The big I of above-mentioned finder is according to different scalable the dwindling of animal.
Be equipped with needed observation, analytical equipments such as microscope 7, cinephotomicrography shooting, micro image analysis system again according to experiment condition and requirement.
Described microcirculation pond 1 and the operating table 9 of placing animal are linked together,, place microcirculation pond 1 then so that obtain the mesenterium of animal from operating table 9.
Center in described microcirculation pond 1 is provided with columniform microcirculation pond inner convex platform 16, and described sponge 3 is the structure of hollow, and described circulatory pool inner convex platform 16 is positioned at wherein; Around multi-turn, its latter end is described perfusion pendular ring 13 to described woven hose 12 on inner convex platform 16 cylindricals of described microcirculation pond.
Woven hose 12 around multi-turn, makes the temperature of liquid in pipe approaching until identical with microcirculation pond 1 interior fluid temperature on microcirculation pond inner convex platform 16.
Described perfusion structure 10 is established the drainage thread that many plying, cotton materials are made, and the one end is located at described checking matter 6 places; The other end is located at 1 below, described microcirculation pond, and this end is placed in the liquid collecting jar 15.
In microcirculation pond 1, put a drainage thread (number of turn decision drainage flow of drainage thread) and form one group of perfusion drainage microcirculation device with the perfusion pendular ring with cotton thread.Drainage thread utilizes siphon principle with checking matter 6 places excess liquid sucking-off, keeps the stable of its humidity.
Microcirculation pond that the utility model adopted 1 adopts organic glass to make, and with chloroform or other adhesives moulding.0.1,0.2,0.5, the thick methacrylate sheet of 0.7cm material is selected:.
It is 12cm that described finder is provided with length, and range is 50 ℃ a thermometer 8.Described thermometer 8 is located at the position of close checking matter 6 on the described microcirculation pond 1, so that the temperature of Real Time Observation testee.
The thermostat water bath 2 that the utility model adopted is the thermostat water bath of digital display type.The thermostat water bath of digital display type is widely used in drying, concentrates, distills, floods chemical reagent, dipping medicine and biological products, also can be used for constant temperature water bath heating and other humid test, is the indispensable instrument of biology, heredity, virus, aquatic products, environmental protection, medicine, health, Biochemical Lab, analysis room, education and scientific research.Its design feature is:
1, operating room's water tank selection stainless steel has superior corrosion resistance;
2, temperature-controlled precision height, numeral demonstration, temperature automatically controlled;
3, easy and simple to handle, safe in utilization.
Specifically, thermostat water bath 2 adopts by the PVG Rong Feng scientific instrument company limited PVG super digital display thermostat water bath of 501A type that glad scientific instrument factory manufactures that jumps.
The lighting of the finder that the utility model adopts will possess the performance of " by force ", " gathering ", " cold ", so the high-pressure sodium lamp of employing 220V, 80W or 12V, 100W nickel tungsten lamp.
Adopt experimental technique of the present utility model:
Open thermostat water bath 2, make microcirculation pond 1 temperature reach temperature required, sponge 3 is put into the pond, again that water-bath is warm tyrode's solution is poured in the pond, with complete submergence sponge 3 degree of being, and insulation balance 10min.
Checking matter 6 (detected animal) is placed on the operating table 9, left side rectus aabdominis at it other (also have and be reported in by the rectus aabdominis of right side) is made the long stringer midriff otch of 6cm, open the abdominal cavity and push the upper abdomen internal organs to right side, select that one section freedom is big, the more rich small intestine intestinal loop of microcirculation, it is tiled on the sponge 3 in the microcirculation pond 1, again perfusion pendular ring (perfusion pendular ring radius is suitable with the mesenterium ring) is posted on mesenterium, at last preservative film 4 is posted on whole perfusion pendular ring and mesenterium.
Preservative film 4 post to mesenterium play insulation, preserve moisture, fixation.And preservative film 4 is good transparent materials, can observe with microscope easily.
In conjunction with the accompanying drawings the utility model has been carried out exemplary description above; obviously the utility model specific implementation is not subjected to the restriction of aforesaid way; as long as adopted the improvement of the various unsubstantialities that method of the present utility model design and technical scheme carry out; or design of the present utility model and technical scheme are directly applied to other occasion without improving, all within protection domain of the present utility model.