CN202022939U - Fatigue loading experimental facility - Google Patents
Fatigue loading experimental facility Download PDFInfo
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- CN202022939U CN202022939U CN2010205372370U CN201020537237U CN202022939U CN 202022939 U CN202022939 U CN 202022939U CN 2010205372370 U CN2010205372370 U CN 2010205372370U CN 201020537237 U CN201020537237 U CN 201020537237U CN 202022939 U CN202022939 U CN 202022939U
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- peristaltic pump
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Abstract
The utility model discloses a fatigue loading experimental facility which is characterized in that a control and data record instrument is connected with a peristaltic pump through a control circuit wire; a water inlet of the peristaltic pump is communicated with an upper opening of a water storage bottle, and a water outlet of the peristaltic pump is communicated with a three-way valve A through a water pipe; one branch pipe of the three-way valve A is communicated with an inlet of an electromagnetic valve, and the other outlet of the electromagnetic valve is communicated with a lower opening of the water storage bottle; the other branch pipe of the three-way valve A is communicated with one end of an elastic water bag in a culture and experiment groove; and the other end of the elastic water bag is communicated with a three-way valve B outside the culture and experiment groove. Due to the fatigue loading experimental facility, mechanism experiments of various types such as controllable horizontal extension and radial extension, or alternated horizontal extension and radial extension can be realized to provide a forcible tool for research of biomembrane materials and variation of a cell under a complex mechanics environment.
Description
Technical field
The utility model relates to a kind of experimental installation of the mechanics of materials, specifically, is a kind of tired loading experimental apparatus that is used for the biomaterial experiment.
Background technology
Along with biological development, cell micro-environment more and more comes into one's own to the influence of the growth of biomaterial biocompatibility and cell, and particularly mechanical stimulation is to the influence research of biomaterial and cell.For this reason, many Experiments of Machanics devices have been invented.
Patent US5348879-A (1994) has invented a kind of device that can apply the twin shaft mechanical stimulation to the cell of cultivation on elastica, be fixed around this device elastica, place a platform below the elastica, this platform is boosted under power wheel drive and is made elastica be stretched.Patent CN 101092595A (experimental apparatus for loading cell through digital controlled mechanical strain, 2007) invented a kind of to being grown in the device that cell on the elastica applies mechanical stimulation, a mechanical top board is arranged below the elastica, produce stretching action by mechanical top board roof pressure elastica.Patent US6107081-A (2000) has invented a kind of device that can apply the single shaft mechanical stimulation to the cell of cultivation on elastica, bar shaped elastica two ends are clamped in this device, one end is fixed on fixed shelf soil, the other end is fixed on the active shelf, the active shelf moves back and forth at power wheel drive lower edge single shaft, thereby along uniaxial extension or elasticity of compression film.Patent CN1932511A (sinusoidal tensile cell loader, 2007) invented a kind of to being grown in the device that cell on the elastica applies the single shaft mechanical stimulation, bar shaped elastica two ends are clamped in this device, one end is fixed on the fixed shelf, the other end is fixed on the active shelf, the active shelf moves back and forth at power wheel drive lower edge single shaft, thereby along uniaxial extension or elasticity of compression film.Patent US6472202-B1 (2002) has invented and has placed a platform in a kind of annular seal space under culture dish bottom surface (elastica), the platform upper surface contacts with the elastica lower surface, elastica above the platform is stretched on this platform upper surface when applying negative pressure like this, the shape that changes the platform upper surface can wait axle or uniaxial extension to elastica, and the difform platform of conversion can apply the various mechanical stimulations that wait axle or single shaft different directions to same sample.Patent CN1846606A (compound mechanical stress cell loading device for simulated joint cavity; 2006) invented and to be fixed on the mechanical platform around its elastica, by mechanical drive tensile elasticity film being grown in the device that cell on the elastica applies multiple mechanical stimulation.
These common shortcomings based on the mechanics loading technique of elastica are exactly: mechanical loading unit all is the stretching of carrying out single type generally, waits axle or uniaxial extension; And the elastica of these devices needs complicated additional components to fix, and complex structure is during production costs.
The utility model content
The purpose of this utility model is the above weak point at existing mechanical loading unit, proposes a kind of mechanical stimulation device to biomaterial fatigue and the research of cell growth effect.
In order to address the above problem, the technical solution of the utility model is: a kind of tired loading experimental apparatus, be made up of peristaltic pump, water pipe, T-valve A, cultivation and experimental tank, control and datalogger etc., it is characterized in that: control is connected peristaltic pump with datalogger by the pilot circuit line; The water-in of peristaltic pump is connected with the water storage bottle is suitable for reading, and its water outlet is connected with T-valve A by water pipe; The arm of T-valve A is communicated with an import of magnetic valve, and another outlet of magnetic valve is connected with water storage bottle end opening; One end of the elasticity water pocket in another arm of T-valve A and cultivation and the experimental tank is connected; The other end of elasticity water pocket is connected with cultivation and experimental tank T-valve B outward.
In the technique scheme, the elasticity water pocket in described cultivation and the experimental tank is with the biomembrane material of experiment outward, and it is fixed on elasticity water pocket outside with retaining clip; The biomembrane material of experiment is before being rolled into tubular or be rolled into to plant behind the tubular and be implanted with cultured cells.
In the technique scheme, the biomembrane material outside of described experiment can also install the crosswise fixed tube additional, radially limit baffle plate.
In the technique scheme, described crosswise fixed tube is rolled into by lubricious thin plates, and the both sides of its opening part are fixed on two stroke motors, and stroke motor can move and stationary fixing on slide rail; Opening part can be located at the place, upper and lower, left and right, and slide rail is corresponding to be placed; Two any parts that radially limit the place, upper and lower, left and right of baffle plate are fixed on two stroke motors; Stroke motor can move and stationary fixing on slide rail; The slide rail of the stroke motor of crosswise fixed tube and two slide rails that radially limit the stroke motor of baffle plate can be provided with the position respectively separately at place, the upper and lower, left and right in cultivation and the experimental tank, also can be the slide rail of two right-angled intersections at a position of cultivation and experimental tank; Stroke motor links to each other with datalogger with control by the pilot circuit line.
Adopt above-mentioned technology,, can obtain waiting the two-way stretch pattern of an axle stretching, uniaxial extension or complicated type, satisfy that the emulation mechanics loads the fatigue experiment test in the biomaterial body by to elastica water pocket and crosswise fixed tube with radially limit the control of baffle plate.Adopt above-mentioned technology, cultivate and experimental tank in developing medium abrim, developing medium can be tissue culture medium, buffered soln etc.; Developing medium is the biomembrane material and the elasticity water pocket of experiment, and the crosswise fixed tube that installs additional of experiment or radially limit the complete submergence of baffle plate.
The utility model has imitated the complicated various of the interior real mechanical environment of body, when can realize culturing cell, realizes the mechanics loading experiment of controlled unidirectional or bi-directional type.Compared with prior art, the utlity model has following beneficial effect:
(1) the utility model is when pair cell applies mechanical stimulation, molecular level dynamic change that can the Real Time Observation viable cell, the containing much information of acquisition.(2) the utility model can realize in same device that controlled cross directional stretch and radial drawing, independent cross directional stretch or independent radial drawing or cross directional stretch and radial drawing replace, Deng the broad variety Experiments of Machanics, for the variation of research cell under complicated mechanical environment provides strong instrument.Move in circles such as the stretching mode of two types of needs and to hocket, this scheme then is difficult to realize, then is easy to realize this function with device of the present invention.(3) the elasticity water pocket of using in the utility model is made simple, does not need extra stationary installation, and good reproducibility.
Description of drawings
Fig. 1 is the biomembrane material and the cell seeding synoptic diagram of experiment of the present utility model.
Fig. 2 is a fatigue loading device synoptic diagram of the present utility model.
Fig. 3 is the tired elasticity water pocket synoptic diagram that loads of radial drawing of the present utility model.
Fig. 4 is the tired elasticity water pocket synoptic diagram that loads of cross directional stretch of the present utility model.
1. retaining clips among the figure; 2. Shi Yan biomembrane material; 3. cultured cells; 4. magnetic valve; 5. water storage bottle; 6. peristaltic pump; 7. water pipe; 8. T-valve A; 9. cultivate and experimental tank; 10. control and datalogger; 11. pilot circuit line; 12. T-valve B; 13. elasticity water pocket; 14. crosswise fixed tube; 15. radially limit baffle plate; 16. slide rail; 17. stroke motor.
Embodiment
Further the utility model is illustrated below in conjunction with drawings and Examples.
Referring to Fig. 1～4, a kind of tired loading experimental apparatus, be made up of peristaltic pump 6, water pipe 7, T-valve A 8, cultivation and experimental tank 9, control and datalogger 10 etc., it is characterized in that: control is connected peristaltic pump 6 with datalogger 10 by pilot circuit line 11; The water-in of peristaltic pump 6 and 5 suitable for reading being connected of water storage bottle, its water outlet is connected with T-valve A 8 by water pipe 7; The arm of T-valve A8 is communicated with an import of magnetic valve 4, and another outlet of magnetic valve 4 is connected with water storage bottle 5 end openings; One end of the elasticity water pocket 10 in another arm of T-valve A 8 and cultivation and the experimental tank 9 is connected; The other end of elasticity water pocket 13 is connected with cultivation and experimental tank 9 T-valve B 12 outward.The elasticity water pocket 13 outer biomembrane materials 2 that are with experiment in cultivation and the experimental tank 9, it is fixed on elasticity water pocket 13 outsides with retaining clip 1; The biomembrane material 2 of experiment is before being rolled into tubular or be rolled into to plant behind the tubular and be implanted with cultured cells 3.Biomembrane material 2 outsides of experiment can also install crosswise fixed tube 14 additional, radially limit baffle plate 15; Crosswise fixed tube 14 is rolled into by lubricious thin plates, and the both sides at its upper opening place are fixed on two stroke motors 17; Two tops that radially limit baffle plate 15 are fixed on two stroke motors 17; Stroke motor 17 can cultivate and the slide rail 16 of two right-angled intersections of experimental tank 9 on move and stationary fixing, stroke motor 17 links to each other with datalogger 10 with control by pilot circuit line 11.Cultivate and experimental tank 9 in the developing medium of tissue culture medium abrim, tissue culture medium is the biomembrane material 2 and the elasticity water pocket 13 of experiment, and the crosswise fixed tube 14 that installs additional of experiment or radially limit baffle plate 15 complete submergences.
Concrete enforcement: this device mainly comprises cell culture unit, tired loading unit, pilot circuit unit, four parts of data record unit.Cell culture unit is finished scleroblast and is cultivated in advance on the biomembrane material 2-collagen/chitosan film of experiment, treat that cell growth reaches 60% and merges the careful collagen-chitin film that takes out in back and roll (the cell face outside) and be enclosed within the cultivation of tired loading unit and the elasticity water pocket 13 in the experimental tank 9, retaining clip 1 is fixedly clamped.Unitary control of pilot circuit and datalogger 10 rotate by pilot circuit line 11 control peristaltic pumps 6, water pump in the water storage bottle 5 is gone out, flow to T-valve A 8, flow into to cultivate then and the elasticity water pocket of experimental tank 9 in 13, close after opening T-valve B 12 exhausts.Control T-valve A 8, when liquid flow is crossed magnetic valve, by the open and close controlling of magnetic valve upper spring sheet cultivate and experimental tank 9 in the diastole of elasticity water pocket 13 or retraction (spring piece presses down, and liquid flow is gone into the water level bottle, the water pocket retraction; Push up flow direction water pocket, water pocket diastole on the spring piece), thus the cell that is grown on the film is carried out fatigue stretching loading.Microcontroller, outputs to power amplification circuit and resupplies rly. for rly. provides switching waveform accurately by self timer, allows rly. under the frequency of setting magnetic valve be controlled, thereby realizes the change of CF.
The unitary control of pilot circuit and datalogger 10 be by pilot circuit line 11, and control peristaltic pump 6 realizes in the elasticity water pockets that 13 rise big and dwindle; And two stroke motors 17 and two stroke motors 17 that radially limit the distance of 15 on baffle plate of control crosswise fixed tube 14 opening and closing sizes, nothing constraint opening and closing, lateral confinement opening and closing, radial constraint opening and closing, the transverse radial that realizes the biomembrane material 2 of testing alternately retrains a series of broad variety mechanics fatigue experiments such as in check such as opening and closing.
The control of data record unit and datalogger 10 binding data analytical resultss, period of supervision fatigue load is to the microtexture of the biomembrane material 2 of experiment and the influence of the scleroblast life behavior of plantation on the biomembrane material 2 of experiment, relation between the fatigue loading/microtexture evolution/cell life behavior of the biomembrane material 2-collagen/chitosan membrane support material of parsing experiment, the design of guiding material and preparation theoretically.
Claims (4)
1. tired loading experimental apparatus, comprise peristaltic pump (6), water pipe (7), T-valve A (8), cultivation and experimental tank (9), control and datalogger (10), it is characterized in that: control is connected peristaltic pump (6) with datalogger (10) by pilot circuit line (11); The water-in of peristaltic pump (6) and suitable for reading being connected of water storage bottle (5), its water outlet is connected with T-valve A (8) by water pipe (7); The arm of T-valve A (8) is communicated with an import of magnetic valve (4), and another outlet of magnetic valve (4) is connected with water storage bottle (5) end opening; One end of the elasticity water pocket (10) in another arm of T-valve A (8) and cultivation and the experimental tank (9) is connected; The other end of elasticity water pocket (13) is connected with cultivation and experimental tank (9) T-valve B (12) outward.
2. a kind of tired loading experimental apparatus according to claim 1 is characterized in that: the outer biomembrane material (2) that is with experiment of the elasticity water pocket (13) in cultivation and the experimental tank (9), it is fixed on elasticity water pocket (13) outside with retaining clip (1).
3. a kind of tired loading experimental apparatus according to claim 1 is characterized in that: the biomembrane material of experiment (2) outside can also install crosswise fixed tube (14) additional, radially limit baffle plate (15).
4. a kind of tired loading experimental apparatus according to claim 3 is characterized in that: crosswise fixed tube (14) is rolled into by lubricious thin plates, and the both sides of its opening part are fixed on two stroke motors (17); Two radially limit baffle plate (15) and are fixed on two stroke motors (17); Stroke motor (17) can be gone up at the slide rail (16) in cultivation and the experimental tank (9) and move and stationary fixing; Stroke motor (17) links to each other with datalogger (10) with control by pilot circuit line (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205372370U CN202022939U (en) | 2010-09-21 | 2010-09-21 | Fatigue loading experimental facility |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205372370U CN202022939U (en) | 2010-09-21 | 2010-09-21 | Fatigue loading experimental facility |
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| CN202022939U true CN202022939U (en) | 2011-11-02 |
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| CN2010205372370U Expired - Fee Related CN202022939U (en) | 2010-09-21 | 2010-09-21 | Fatigue loading experimental facility |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974417A (en) * | 2010-09-21 | 2011-02-16 | 重庆文理学院 | Fatigue loading experimental device and process thereof |
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2010
- 2010-09-21 CN CN2010205372370U patent/CN202022939U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974417A (en) * | 2010-09-21 | 2011-02-16 | 重庆文理学院 | Fatigue loading experimental device and process thereof |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111102 Termination date: 20120921 |