CN201828566U - Bilirubin content detection kit - Google Patents
Bilirubin content detection kit Download PDFInfo
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- CN201828566U CN201828566U CN2010205091292U CN201020509129U CN201828566U CN 201828566 U CN201828566 U CN 201828566U CN 2010205091292 U CN2010205091292 U CN 2010205091292U CN 201020509129 U CN201020509129 U CN 201020509129U CN 201828566 U CN201828566 U CN 201828566U
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- reagent
- detection kit
- bilirubin
- cholerythrin
- reagent unit
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Abstract
The utility model provides a bilirubin content detection kit which comprises a kit cover, a kit body and three agent units arranged on in the kit body, wherein the three agent units are respectively provided with at least one independent sealed container. The detection box has compact structure, is convenient to disassemble and assemble, simple to operate, and flexible to apply, has good stability, can meet requirements of different detection objects, and has low cost. The detection box can be simultaneously used for quickly detecting direct bilirubin and total bilirubin.
Description
Technical field
The utility model belongs to medical science in-vitro diagnosis field, specifically is a kind of novel content of bilirubin detection kit that can be used for detecting bilirubin direct and total bilirubin content in the human blood simultaneously.
Background technology
Cholerythrin is the catabolite of haemachrome molecule in haemoglobin and the hemoprotein, because its mensuration to liver function, especially the discriminating of jaundice is significant, is the ancient and important problem of clinical medicine always, becomes one of normal project of doing in the clinical biochemical laboratory at present especially.Improved diazo reagent and be used for the mensuration of serum mesobilirubin from VandenBergh in 1916.From then on cholerythrin is named as direct reaction, hemobilirubin according to the pattern that itself and diazo reagent react, and sum of the two claims total bilirubin.
Be usually used in clinical detection cholerythrin method at present diazonium method, oxidation enzyme process, chemical oxidization method etc. are arranged.Wherein the diazo reagent method is historical the longest method, and principle is diazo salt and cholerythrin combination, under certain wavelength, and the variation that can produce absorbance.This method has the improvement of numerous species, and the main method of using has M-EShi method J-GShi method and improved method thereof.All be to generate diazo reagent by sulfanilic acid and sodium nitrite reaction then to react with cholerythrin.This method is swift in response, and is easy and simple to handle, and cost is lower, pretends to the high reagent of a kind of cost performance to be widely used.
The utility model content
The purpose of this utility model is to provide that a kind of cost is lower, stable performance, easy to use, and can satisfy the novel content of bilirubin detection kit that clinical bilirubin direct and total bilirubin detect simultaneously, can be formulated as bilirubin direct testing liquid and total bilirubin testing liquid, convenient clinical use according to different needs.
The utility model is achieved through the following technical solutions:
The utility model provides a kind of content of bilirubin detection kit, and three reagent unit that comprise lid, box body and place box body, described reagent unit comprise that respectively at least one is loaded with the independent sealed container of liquid.Specifically, three reagent unit are loaded with reagent 1 liquid, accelerator liquid and reagent 2 liquid respectively.
Described box body can be square shape or rectangular shape, and lid has shape correspondingly; Lid can monolaterally be connected on the box body, or separate with box body.
The autonomous container of described three reagent unit is a reagent bottle, and described reagent bottle is made up of bottle and bottle cap.Reagent bottle can be cylindrical, four prism type or other shapes.
Described three reagent unit are placed independently of one another, or form one by the autonomous container outer wall is directly continuous, or connect as one by support, and described support is selected from ring support or platform-type support.
The utility model content of bilirubin detection kit kit also includes fixture, and fixture is filled in around the independent sealed container, and described fixture is selected from a kind of of polyfoam, sponge.Fixture is filled in around the independent sealed container, plays effect fixing, antidetonation.
Described reagent 1 liquid comprises: the phosphoric acid of Ph2.0-4.0 or citrate buffer solution, content are 0.1mmol/L-2.0mmol/L, stabilizing agent glycerine or trehalose 1%-10% and sulfanilic acid 10mmol/L-20mmol/L.
Described accelerator liquid comprises dimethyl sulfoxide 20-70%, tween 5%-15%, polyoxyethylene laurel ether 5%-10%.
Comprise sodium nitrite 2mmol/L-10mmol/L in described reagent 2 liquid, stabilizing agent glycerine or trehalose 1%-10%.
Wherein to be mixed into volume ratio that working fluid can be used at 1: 1 be the bilirubin direct content detection for reagent 1 and reagent 2, and reagent 1, accelerator and reagent 2 are mixed into working fluid with volume ratio and can be used for the total bilirubin content detection at 1: 1: 1.Can satisfy different detection demands easily.
The design concept of the utility model kit is as follows: reagent 1 with can generate diazo salt rapidly after reagent 2 mixes, can be used for bilirubin direct and detect; Reagent 1, accelerator and after reagent 2 mixed, reagent 1 generated diazo salts fast with reagent 2, this diazo salt can be used for total bilirubin detection in the sample in the presence of accelerator.
After being formulated as working fluid, present commercially available diazonium method detectable generally can stablize 6-8 hour in room temperature, experimental results show that this novel cholerythrin detection kit has higher stability, be formulated as respectively that at room temperature to place after 12 hours accuracy in detection and linearity behind bilirubin direct working fluid and the total bilirubin working fluid still better.In addition, the utility model kit has been integrated bilirubin direct and total bilirubin, can be used for the detection demand of straight courage and total two projects of courage simultaneously, and the user of service can reduce the waste of reagent to a certain extent according to detecting needs preparation working fluid.And cost is lower, and is simple to operate.In addition, prove that by experiment this method and vanadate oxidizing process detect cholerythrin and have stronger correlativity.
Below the utility model is described in further detail by the drawings and specific embodiments, and embodiment provides with the purpose of giving an example, and does not constitute any restriction of the present utility model.
Description of drawings
Three-dimensional structure diagram when Fig. 1 opens for the utility model lid.
Fig. 2 is three reagent unit laying state synoptic diagram in airtight box body.
Fig. 3 is three reagent unit of one for being connected by the autonomous container outer wall.
Fig. 4 is the sectional view of Fig. 3 along the B-B line.
Three reagent unit of Fig. 5 for adopting ring support to connect as one.
Three reagent unit of Fig. 6 for adopting platform-type support to connect as one.
Wherein, 1 is lid, and 2 is box body, and 3 is first reagent unit, and 4 is second reagent unit, and 5 is the 3rd reagent unit, and 6 is fixture, and 7 for connecting support.
Embodiment
As Fig. 1 and Fig. 2, the utility model content of bilirubin detection kit, comprise lid 1 and box body 2, and first reagent unit 3, second reagent unit 4, the 3rd reagent unit 5, each reagent unit is made up of a sealable reagent bottle, and three reagent unit are loaded with pretreatment liquid respectively, and NBT method fructosamine is measured reagent, albumin measuring reagent, the mentioned reagent sequence of unit is positioned in the box body 2.
The utility model lid 1 and box body 2 can be airtight, use front opening.
The independent sealed container of three reagent unit of the utility model content of bilirubin detection kit can be connected with each other, form one, as Fig. 3 and Fig. 4, the outer wall of forming the independent sealed container of first reagent unit 3, second reagent unit 4, the 3rd reagent unit 5 directly links to each other and forms one.
The independent sealed container of three reagent unit of the utility model content of bilirubin detection kit can be connected with each other, form one, as Fig. 5 and Fig. 6, the outer wall of forming the independent sealed container of first reagent unit 3, second reagent unit 4, the 3rd reagent unit 5 connects as one by support 7, Fig. 5 and Fig. 6 are the support of two kinds of different structures and shape, are respectively ring support and platform-type support.
Claims (7)
1. a content of bilirubin detection kit is characterized in that, three reagent unit that comprise lid, box body and place box body, described reagent unit comprise that respectively at least one is loaded with the independent sealed container of liquid reagent.
2. a kind of cholerythrin detection kit as claimed in claim 1 is characterized in that the autonomous container of described three reagent unit is a reagent bottle, and described reagent bottle is made up of bottle and bottle cap.
3. a kind of cholerythrin detection kit as claimed in claim 1 is characterized in that, described three reagent unit are placed independently of one another.
4. a kind of cholerythrin detection kit as claimed in claim 1 is characterized in that, described three reagent unit directly link to each other by the autonomous container outer wall and form one.
5. a kind of cholerythrin detection kit as claimed in claim 1 is characterized in that, described three reagent unit connect as one by support.
6. a kind of cholerythrin detection kit as claimed in claim 5 is characterized in that described support is selected from ring support or platform-type support.
7. a kind of cholerythrin detection kit as claimed in claim 1 is characterized in that described kit also includes fixture, and fixture is filled in around the independent sealed container, and described fixture is selected from a kind of of polyfoam, sponge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205091292U CN201828566U (en) | 2010-08-26 | 2010-08-26 | Bilirubin content detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205091292U CN201828566U (en) | 2010-08-26 | 2010-08-26 | Bilirubin content detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201828566U true CN201828566U (en) | 2011-05-11 |
Family
ID=43967092
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010205091292U Expired - Lifetime CN201828566U (en) | 2010-08-26 | 2010-08-26 | Bilirubin content detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201828566U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102226768A (en) * | 2011-03-17 | 2011-10-26 | 郑州兰森生物技术有限公司 | Reagent for determining total bilirubin by sodium nitrite oxidation method |
| CN110261625A (en) * | 2019-07-15 | 2019-09-20 | 三诺生物传感股份有限公司 | A kind of bilirubin multiplexed detection reagents box and its application method |
| CN110274881A (en) * | 2018-03-14 | 2019-09-24 | 济南煊赫生物科技有限公司 | A kind of stabilization, total bilirubin (enzymatic measurement) detection reagent of strong antijamming capability and detection method |
-
2010
- 2010-08-26 CN CN2010205091292U patent/CN201828566U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102226768A (en) * | 2011-03-17 | 2011-10-26 | 郑州兰森生物技术有限公司 | Reagent for determining total bilirubin by sodium nitrite oxidation method |
| CN102226768B (en) * | 2011-03-17 | 2012-10-03 | 郑州兰森生物技术有限公司 | Reagent for determining total bilirubin by sodium nitrite oxidation method |
| CN110274881A (en) * | 2018-03-14 | 2019-09-24 | 济南煊赫生物科技有限公司 | A kind of stabilization, total bilirubin (enzymatic measurement) detection reagent of strong antijamming capability and detection method |
| CN110261625A (en) * | 2019-07-15 | 2019-09-20 | 三诺生物传感股份有限公司 | A kind of bilirubin multiplexed detection reagents box and its application method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 201204 Shanghai city Pudong New Area Lianxi Road No. 1210 Building 2 Patentee after: Shanghai Zhicheng Biological Technology Co.,Ltd. Address before: 201204 Shanghai city Pudong New Area Lianxi Road No. 1210 Building 2 Patentee before: Shanghai Zhicheng Biotechnology Co., Ltd. |
|
| C56 | Change in the name or address of the patentee | ||
| CP02 | Change in the address of a patent holder |
Address after: 201204, Lane 3399, Lane 6, Kang Xin Road, Shanghai, Pudong New Area Patentee after: Shanghai Zhicheng Biological Technology Co.,Ltd. Address before: 201204 Shanghai city Pudong New Area Lianxi Road No. 1210 Building 2 Patentee before: Shanghai Zhicheng Biological Technology Co.,Ltd. |
|
| CP01 | Change in the name or title of a patent holder |
Address after: 201204, Lane 3399, Lane 6, Kang Xin Road, Shanghai, Pudong New Area Patentee after: SHANGHAI ZHICHENG BIOTECHNOLOGY CO., LTD. Address before: 201204, Lane 3399, Lane 6, Kang Xin Road, Shanghai, Pudong New Area Patentee before: Shanghai Zhicheng Biological Technology Co.,Ltd. |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20110511 |