CN201799137U

CN201799137U - Quick detach type large-volume prepared chromatographic column

Info

Publication number: CN201799137U
Application number: CN2010202917703U
Authority: CN
Inventors: 周胜; 徐慧; 曾虎
Original assignee: LISURE SCIENCE (SUZHOU) CO Ltd
Current assignee: LISURE SCIENCE (SUZHOU) CO Ltd
Priority date: 2010-08-13
Filing date: 2010-08-13
Publication date: 2011-04-20
Anticipated expiration: 2020-08-13

Abstract

The utility model relates to a quick detach type large-volume prepared chromatographic column which comprises a column body with pipe-shaped column wall. The top end and bottom end of the column body are provided with an upper flange, a lower flange and a pipe sealing ring which is used for connecting the sealed column body with the upper flange and the lower flange. The upper flange is connected with the lower flange through a double end stud. The top end of the upper flange is provided with an upper cover plate with an upper ferrule external thread pipe welding joint and a pressure release opening at the top part. Furthermore a shunting plate is provided in a chamber of the upper cover plate. The bottom end of the lower flange is provided with a lower cover plate with a lower ferrule external thread pipe welding joint at the top part. An inner outlet of the lower cover plate is provided with a sieve plate which is fixed by an end shrink opening. A part between the upper flange and the upper cover plate, and a part between the lower flange and the lower cover plate are sealed through the matching between a collar clamp sealing ring and a collar clamp. The quick detach type large-volume prepared chromatographic column has the following advantages: firm whole body, convenient movement; 2. quick filler or sample loading; 3. uniform fluid distribution through experiment, and high column efficiency; and 4. reasonable fabrication cost.

Description

Quick-release type large-volume preparative chromatographic column

Technical field:

The utility model relates to a kind of quick-release type large-volume preparative chromatographic column, is used for preparative chromatography separation and purification and extraction field, can Fast Installation clean, and is suitable for pressing among the overwhelming majority column separating purification of filler to prepare requirement, also can use as extraction column simultaneously.

Background technology:

Chromatogram is a kind of compartment analysis means, separation is a core, therefore the chromatographic column of undertaking centrifugation is the heart of chromatographic system, requirement to chromatographic column is that post is imitated height, selectivity is good, analysis speed is fast etc., simultaneously, when also wishing in use large-scale preparative chromatography post, can observe separation and purification situation in the post.

For the medium-and-large-sized preparative chromatography post more than the internal diameter 50mm, need to consider the uniform distribution of liquid in post, and fluid/sample allocation of parameters is the key that effective post separates, it is the linear key technology of amplifying of chromatogram, the withstand voltage of these medium-and-large-sized preparative chromatography posts must reach 20bar (2MPa) simultaneously, satisfies the instructions for use of pressing filler in the overwhelming majority.

And the domestic horizontal proof pressure of the post observed of glass material has only 0.3MPa (3bar) at present, external famous manufacturer adopts special glass as step fine jade (BUCHI), heavy caliber 100mm is above also has only 2MPa, and price is very expensive, installation and removal are inconvenience very, and the post pole of glass material is easy to generate wall effect.

Summary of the invention:

In order to solve the problems of the technologies described above, the utility model provides a kind of quick-release type large-volume preparative chromatographic column

The technical scheme that its technical problem that solves the utility model adopts is: a kind of quick-release type large-volume preparative chromatographic column, comprise that cylinder wall is the cylinder of pipeline, the top and the bottom of described cylinder are provided with upper flange, lower flange and be used for connecting sealing cylinder and upper flange, the seal for pipe joints circle of lower flange, connect by studs between described upper flange and the lower flange, described upper flange top is equipped with the upper cover plate that the top has clip-sleeve external-thread pipe welding point and relief hole, and in the cavity of described upper cover plate, be provided with flow distribution plate, described lower flange bottom is equipped with the top and has the lower cover of clip-sleeve external-thread pipe welding point down, the inner outlet place of described lower cover is provided with sieve plate, described sieve plate is fixed described upper flange and upper cover plate by the end reducing, be provided with clip sealing ring and clip between lower flange and the lower cover.

In order to increase withstand voltage effect, further: described clip is served as reasons and is appointed structure.

For the ease of fixing and placement, further: described quick-release type large-volume preparative chromatographic column is connected by the fixed head of the contiguous block on the upper flange with the column holder top, and is positioned in the cavity of column holder.

Move for the ease of integral body, further: described column holder bottom is equipped with castor.

The utility model is at the use problem and the shortcoming of the medium-and-large-sized preparative chromatography post more than the internal diameter 50mm in the past, and design and manufacturing have solved following technical problem:

1. Fast Installation and dismounting:

Upper and lower side adopts the quick clip of health level and by appointing version, withstand voltagely can reach 20bar (2MPa), simultaneously opening and column diameter one (not having necking down).

2. can select transparent or semitransparent material to observe between cylinder:

Cylinder adopts health grade stainless steel extremity piece up and down, standard series tubing (material can be selected glass/quartz/fluoroplastics (PVDF or PFA)/PP etc.) is adopted in middle design, can select according to operational characteristic, the centre compresses with bolt, factory installs, withstand voltage height, reliable (adopt translucent PFA or PVDF material at present, experimental results show that and can well eliminate wall effect).

3. the even separate system of particular design guarantees the even velocity of flow from inlet tube to whole column section.

4. every kind of post has all designed column holder, and the above post of 100mm has been equipped with the removable pylon car of foot wheel.

5. the filler manual pneumatic tightly installs.

The utility model has following advantage through experiment and customer authentication:

1. firmly whole, conveniently moving;

2. can load and unload filler or sample fast;

3. experimental verification fluid distributed uniform, post is imitated high;

4. cost is reasonable.

Description of drawings:

Fig. 1 is an overall structure schematic diagram of the present utility model.

The schematic diagram of Fig. 2 mobile pylon car for the utility model is fixed with.

Fig. 3 is the structural representation of clip.

Fig. 4 is the use schematic diagram of the utility model filling material mechanism.

Among the figure: 1, pipeline; 2, cylinder; 3, upper flange; 4, lower flange; 5, seal for pipe joints circle; 6, studs; 7, go up clip-sleeve external-thread pipe welding point; 8, relief hole; 9, upper cover plate; 10, flow distribution plate; 11, following clip-sleeve external-thread pipe welding point; 12, lower cover; 13, sieve plate; 14, end reducing; 15, clip sealing ring; 16, clip; 17, contiguous block; 18, column holder; 19, fixed head; 20, castor; 21, gas cylinder; 22, valve inside; 23, adapter.

The specific embodiment:

Below in conjunction with drawings and Examples the utility model is further specified.

A kind of quick-release type large-volume preparative chromatographic column as shown in Figure 1, comprise that cylinder wall is the cylinder 2 of pipeline 1, adopt translucent PFA or PVDF material, use this kind material can not only conveniently observe separation and purification situation in the post, simultaneously can eliminate wall effect, top and bottom at described cylinder 2 are provided with upper flange 3, lower flange 4 and be used for connecting sealing cylinder 2 and upper flange 3, the seal for pipe joints circle 5 of lower flange 4, connect by studs 6 between described upper flange 3 and the lower flange 4, described upper flange 3 tops are equipped with the upper cover plate 9 that the top has clip-sleeve external-thread pipe welding point 7 and relief hole 8, and in the cavity of described upper cover plate 9, be provided with flow distribution plate 10, described lower flange 4 bottoms are equipped with the top and have the lower cover 12 of clip-sleeve external-thread pipe welding point 11 down, the inner outlet place of described lower cover 12 is provided with sieve plate 13, described sieve plate 13 is fixing by end reducing 14, described upper flange 3 and upper cover plate 9, pass through clip sealing ring 15 and clip 16 fitted seal between lower flange 4 and the lower cover 12, tighten after clip is inserted in and promptly reach fixing and sealing effectiveness, in order to increase withstand voltage effect, described clip 16 can use by appointing structure (as Fig. 3), thereby make it withstand voltagely can reach 20bar (2MPa), and opening and column diameter one (do not have necking down, the convenient filler of installing, eliminate wall effect), simultaneously, also a contiguous block 17 can be installed on preparative chromatography post top, it is placed in the cavity of column holder 18, link into an integrated entity by the fixed head 19 of the contiguous block on its upper flange 17 then with column holder 18 tops, described column holder 18 bottoms also are equipped with castor 20 (as shown in Figure 2), not only be convenient to fixed placement by this kind design, also be convenient to simultaneously move and install.

In conjunction with shown in Figure 4, it is as follows to introduce dress column method of the present utility model:

1, earlier with the solid filler of cylinder 2 and upper cover plate 9, uses then both are fixed together with clip 16;

2, the structure by Fig. 3 couples together gas cylinder 21, valve inside 22, adapter 23 and quick-release type large-volume preparative chromatographic column;

3, be forced into filler consolidation (filler in the upper cover plate 9 also has living space after all being pressed in the cylinder 2, then can pull down to be forced into also residual fillers in the upper cover plate 9 again after upper cover plate 9 is adorned the back) with gas cylinder 21

4, take off upper cover plate, remove residual filler,, fix with clip 16 again, promptly install flow distribution plate 10 fit ons.

Claims

1. quick-release type large-volume preparative chromatographic column, it is characterized in that: described quick-release type large-volume preparative chromatographic column comprises that cylinder wall is the cylinder (2) of pipeline (1), the top of described cylinder (2) and bottom are provided with upper flange (3), lower flange (4) and be used for connecting sealing cylinder (2) and upper flange (3), the seal for pipe joints circle (5) of lower flange (4), connect by studs (6) between described upper flange (3) and the lower flange (4), described upper flange (3) top is equipped with the upper cover plate (9) that the top has clip-sleeve external-thread pipe welding point (7) and relief hole (8), and in the cavity of described upper cover plate (9), be provided with flow distribution plate (10), described lower flange (4) bottom is equipped with the top and has the lower cover (12) of clip-sleeve external-thread pipe welding point (11) down, the inner outlet place of described lower cover (12) is provided with sieve plate (13), described sieve plate (13) is fixing by end reducing (14), described upper flange (3) and upper cover plate (9), be provided with clip sealing ring (15) and clip (16) between lower flange (4) and the lower cover (12).

2. quick-release type large-volume preparative chromatographic column according to claim 1 is characterized in that: described clip (16) is served as reasons and is appointed structure.

3. quick-release type large-volume preparative chromatographic column according to claim 1, it is characterized in that: described preparative chromatography post is connected with the fixed head (19) at column holder (18) top by the contiguous block (17) on the upper flange (3), and is positioned in the cavity of column holder (18).

4. quick-release type large-volume preparative chromatographic column according to claim 1 is characterized in that: described column holder (18) bottom is equipped with castor (20).