CN201780273U - Automatic protein crystal high-pass sample observation system - Google Patents
Automatic protein crystal high-pass sample observation system Download PDFInfo
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- CN201780273U CN201780273U CN2010202203582U CN201020220358U CN201780273U CN 201780273 U CN201780273 U CN 201780273U CN 2010202203582 U CN2010202203582 U CN 2010202203582U CN 201020220358 U CN201020220358 U CN 201020220358U CN 201780273 U CN201780273 U CN 201780273U
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Abstract
The utility model discloses an automatic protein crystal high-pass sample observation system, which comprises a translation platform, a movement controller and a light source controller which are connected with one another. The automatic protein crystal high-pass sample observation system solves problems that some laboratory crystal lighting sources are difficult to regulate and control, and crystals and crystal boundaries cannot be distinguished. The automatic protein crystal high-pass sample observation system adopts the multilayer section focusing technique instead of the simple single-sided automatic focusing technique for particularity of the crystals and crystal growth environments in strategy of image photographing and collection, increases accuracy and precision of data acquisition, and records three-dimensional spatial sizes of the crystals.
Description
Technical field
The utility model relates to a kind of high flux sample automatic observing system, specially refers to a kind of protein crystal high flux sample automatic observing system.
Background technology
Along with science and technology development " the back genomics epoch " up till now, bio-science research has all had great leap on scale and speed, and structure biology is no exception.Structure biology research has strided forward the structural genomics epoch, countries in the world have started the structural genomics plan one after another, set up high flux, large-scale albumen clone, expression, purifying, crystallization and crystallographic structural analysis platform, in order to carry out efficient, the research work of fast protein 26S Proteasome Structure and Function.Traditional albumin crystal screens and is viewed as manual operations, and general Study personnel arrive hundreds of kind crystallization condition for a protein screening tens; Along with the foundation of a lot of novel crystallization conditions and method, thousands of kinds of crystallization conditions of increasing protein requirement screening, for manual operations, increasing of screening conditions increased the weight of researchist's workload and degree of accuracy greatly; Manual operations speed is slow, and sample is volatile, all can have a strong impact on experimental result, and is particularly all the more so for receiving upgrading ultra-small volume operation.The albumin crystal screening operation time itself is long, is easy to fatigue and causes and add wrong screening reagent, for microscopically observation crystal condition, also be like this, long-time, the manual observation of bulk crystallization condition, be easy to make mistakes, accurately write down each crystallization condition develop in time condition may.But a large amount of screening conditions and the uncertainty of albumin crystal growth time make crystal growth and observation become the work of a time and effort consuming.
For the structure biology laboratory of being engaged in the research of a large amount of albumin crystal structure elucidations, high-level efficiency is robotization crystal growth cheaply and the recording geometry condition that necessitates again simultaneously.Though the self-reacting device of more existing crystal growths and observation comes out on the international market at present, the function of these commercial apparatuss is usually comparatively single, and the crystal growth and the recording geometry that be equipped with a whole set of robotization need a large amount of fund inputs.In addition, these systems usually can not suit laboratory study personnel's actual needs well, in the research and development practical study personnel participate in less, the robotization that covets, black box operation is more, causes many possibility of result to be left in the basket, even by fault processing.Before automated production comes out, all be manual operations to the monitoring and the record of crystal structure processes such as albumen, to moderate cost, the demand of the crystal detection system of precision, accuracy and real-time context of detection has seemed extremely urgent.
Existing microscope mainly contains following three shortcomings:
1. can't control automatically and take pictures, some in addition can't take pictures.
2. can't continuous recording crystal growth situation, can't form the crystal growth storehouse of system.
3. illumination be can't regulate automatically and various crystal slabs or various crystal state adapted to.
The utility model content
The technical matters that the utility model solved provides a kind of protein crystal high flux sample automatic observing system, solved laboratory crystal lighting source regulation and control difficulty and can't the discrimination crystal and the problem on border.
Technical scheme is as follows:
A kind of protein crystal high flux sample automatic observing system, comprising: translation stage, motion controller and light source controller, described translation stage, motion controller and light source controller are connected to each other.
Further: also comprise the general-purpose serial bus USB hub, client is connected to described translation stage and light source controller by described usb hub, and described client is connected with described motion controller by the RS232 serial line interface.
Further: be connected with at least one client.
Further: described motion controller receives the instruction that described client is sent here by described RS232 serial line interface, and described motion controller drives described translation stage according to described instruction and moves.
Further: described translation stage is returned to described client to the sampled signal that generates by described usb hub.
Further: also comprise the control signal divider, client is by being connected to described motion controller and light source controller.
Further: comprise at least one group of described translation stage, motion controller and light source controller, described translation stage, motion controller and light source controller in every group are connected to each other; Described control signal divider is connected respectively with light source controller with every group of described motion controller.
Further: described translation stage is returned to described client to the sampled signal that generates by described control signal divider.
Further: described motion controller receives the instruction that described client is sent by described control signal divider, and described motion controller drives described translation stage according to described instruction.
Further: described motion controller is connected with network respectively with light source controller.
Technique effect:
1, the picture quality of present most commercial products is unsatisfactory, the utility model is aspect light source and imaging, for example, take LED to replace the traditional cold light source technology, utilize computer digital control light source, make the regulation automatization of light source, solved some laboratory crystal lighting sources regulation and control difficulty and can't the discrimination crystal and the problem on border.
2, on the image photographic collection strategy, at the singularity of crystal and growing environment thereof, adopt multilayer tangent plane focusing technology, rather than simple single face automatic focus technology, improve the accuracy and the accuracy of data acquisition, write down the three dimensions size of crystal.
3, database and network management system advanced and reasonable design can carry out Long-distance Control and visit, can significantly reduce execute-in-place, and this is particularly necessary for the observation of (in the cold house) crystal growth under specific condition.Management system can also add or delete the number of observation instrument and corresponding control system as required under the prerequisite that keeps legacy data management system (LIMS).
4, data management system has professional platform independence, can freely transplant under Linux and Windows system, does not need the update routine code.And this system both can be used as an intact part and independently existed, and also can be incorporated among other LIMS.
5, this system has opening and versatility preferably, can transplant, revise and be applied in various Study on Crystals Growth (as the micromolecule crystal growth) or other the similar experiments (cultivating during robotization observes as zebra fish-egg and embryo, frog's egg and embryo even cell or tissue) easily.
Description of drawings
Fig. 1 is multi-client of the present utility model-single worktable scheme synoptic diagram;
Fig. 2 is the wiring diagram of multi-client of the present utility model-single worktable scheme;
Fig. 3 is single client of the present utility model-many worktable scheme synoptic diagram;
Fig. 4 is the wiring diagram of single client of the present utility model-many worktable scheme;
Fig. 5 is a crystal cut synoptic diagram in the utility model;
Fig. 6 is that section merges synoptic diagram in the utility model.
Embodiment
The utility model has changed the experiment model of studying protein crystal in the past fully, furtherly, microscope in the existing experiment model manually taken pictures change remote auto control into and take pictures, and energy continuous recording crystal growth situation, the crystal growth storehouse of formation system, and can regulate the parameter of taking pictures automatically with different environment of crystal growth and state at different crystal culture plates, and realized networking, long-range, make the user not need to place oneself in the midst of and come deal with data in the cold house, every cover machine can support a plurality of users to use observation data simultaneously, every cover machine is supported a plurality of clients, can operate in different positions.The utility model typical application mode is multi-client-single worktable and single client-many worktable, can certainly be multi-client-many worktable mode.Client herein can be access devices such as computer terminal, and worktable is to general designation of the present utility model, and the computer terminal can be by mouse and keyboard input instruction.
Below with reference to accompanying drawing and preferred embodiment, the technical solution of the utility model is done detailed description.
As shown in Figure 1, be multi-client of the present utility model-single worktable scheme synoptic diagram.In this programme, comprising: client 11, client 12 and worktable 13, client 11 is connected with worktable by lead with client 12; Client 11 is arranged on divider wall inside, and client 12 is arranged on the divider wall outside, and operating personnel can issue operational order by client 11 and client 12 and give worktable 13, realize the observation at any time to crystal growth.
As shown in Figure 2, be the wiring diagram of multi-client of the present utility model-single worktable scheme.Its structure comprises: translation stage 22, motion controller 23 and light source controller 21, and translation stage 22, motion controller 23 and light source controller 21 are connected to each other; For the ease of linking to each other with the outside, be provided with USB (universal serial bus) (USB, Universal Serial BUS) hub 24, client 11 or client 12 are connected respectively to translation stage 22 and light source controller 21 by this usb hub 24, and client 11 or client 12 are connected with motion controller 23 by the RS232 serial line interface.
Motion controller 23 receives the instruction that client 11 or 12 is sent by the RS232 serial line interface, and motion controller 23 powers up according to instruction, the actuation movement machine operation, and then drive the work of translation stage 22.
Light source controller 21 receives client 11 or 12 instructions that send by usb hub 24, the instruction control relay that light source controller 21 bases receive, thus reach the purpose of controlling light source controller 21.
The three-dimensional many focal plane sections of 22 pairs of crystal by adopting of translation stage are taken pictures and record, sample respectively at the different interfaces of crystal growth, the sampled signal that generates is passed back client 11 or 12 by usb hub 24, and then generate hologram image, have avoided the omission of information.
As shown in Figure 3, be single client of the present utility model-many worktable synoptic diagram.In this programme, comprising: client 31 and worktable 1～N, client 31 is connected with worktable 1～N respectively; Operating personnel can issue operational order by client 31 and give worktable 1～N, realize worktable 1～N monitoring observation at any time of crystal growth down.
As shown in Figure 4, be the wiring diagram of single client of the present utility model-many worktable scheme.Its structure comprises: n translation stage 22, motion controller 23 and light source controller 21, and translation stage 22, motion controller 23 and light source controller 21 are connected to each other; For the ease of linking to each other with the outside, be provided with control signal divider 41, client 11 is connected to motion controller 23 and light source controller 21 by this control signal divider 41.
Motion controller 23 receives the instruction that clients 11 are sent by control signal divider 41, and motion controller 23 powers up according to instruction, the actuation movement machine operation, and then drive the work of translation stage 22.
Light source controller 21 receives the instruction that client 11 sends by control signal divider 41, the instruction control relay that light source controller 21 bases receive, thus reach the purpose of controlling light source controller 21.
The three-dimensional many focal plane sections of 22 pairs of crystal by adopting of translation stage are taken pictures and record, sample respectively at the different interfaces of crystal growth, the sampled signal that generates is passed back client 11 by control signal divider 41, and then generate hologram image, have avoided the omission of information.
Above-mentioned motion controller 23 can be connected with network respectively with light source controller 21, the user can utilize client 11 to pass through network, be remotely logged into the utility model, thereby carry out Long-distance Control, avoid laboratory operation and destroy the growth or the breaking test condition of some crystal.
As shown in Figure 5 and Figure 6, the utility model adopts and divides eager chip technology, and crystal is carried out the three-dimensional sampling, and then the synthetic hologram image, has avoided the information omission.Because crystal itself grows in the solution, is the 3 D stereo shape, and because its color and transparency and its cultivation mother liquor are very approaching, thereby general image processing algorithm can not correctly be discerned.
Realizing the running of protein crystal high flux sample automatic observing system, is by the supporting software of special production, only needs the user to import corresponding information, promptly can turn round automatically, observes crystal and Taking Pictures recording.
The use step is as follows:
The first step: start-up control computing machine and control assembly;
Second step: start software;
The 3rd step: software connects each controller, sends initialization directive to each controller;
The 4th step: after controller and working position are in place, feed back to software, start working;
The 5th step: the user imports corresponding information, clicks the operation button, promptly can begin to observe crystal and Taking Pictures recording automatically.
Total system has realized operated from a distance, real time record and monitoring based on advanced person's express network and database; Detection system gathered optics amplification, programmable light source, data imaging, multinomial technology such as control location and teledata processing automatically, and system has realized at a high speed, trace, the function used more, and the three-dimensional motion precision of system is controlled in 5 microns; User interface of software is good.
Claims (10)
1. a protein crystal high flux sample automatic observing system is characterized in that, comprising: translation stage, motion controller and light source controller, described translation stage, motion controller and light source controller are connected to each other.
2. protein crystal high flux sample automatic observing system as claimed in claim 1, it is characterized in that: also comprise the general-purpose serial bus USB hub, client is connected to described translation stage and light source controller by described usb hub, and described client is connected with described motion controller by the RS232 serial line interface.
3. protein crystal high flux sample automatic observing system as claimed in claim 2 is characterized in that: be connected with at least one client.
4. protein crystal high flux sample automatic observing system as claimed in claim 2, it is characterized in that: described motion controller receives the instruction that described client is sent here by described RS232 serial line interface, and described motion controller drives described translation stage according to described instruction and moves.
5. protein crystal high flux sample automatic observing system as claimed in claim 2 is characterized in that: described translation stage is returned to described client to the sampled signal that generates by described usb hub.
6. protein crystal high flux sample automatic observing system as claimed in claim 1 is characterized in that: also comprise the control signal divider, client is by being connected to described motion controller and light source controller.
7. protein crystal high flux sample automatic observing system as claimed in claim 6, it is characterized in that: comprise at least one group of described translation stage, motion controller and light source controller, described translation stage, motion controller and light source controller in every group are connected to each other; Described control signal divider is connected respectively with light source controller with every group of described motion controller.
8. protein crystal high flux sample automatic observing system as claimed in claim 6 is characterized in that: described translation stage is returned to described client to the sampled signal that generates by described control signal divider.
9. protein crystal high flux sample automatic observing system as claimed in claim 6, it is characterized in that: described motion controller receives the instruction that described client is sent by described control signal divider, and described motion controller drives described translation stage according to described instruction.
10. as each described protein crystal high flux sample automatic observing system of claim 1 to 9, it is characterized in that: described motion controller is connected with network respectively with light source controller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2010202203582U CN201780273U (en) | 2010-06-09 | 2010-06-09 | Automatic protein crystal high-pass sample observation system |
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| CN2010202203582U CN201780273U (en) | 2010-06-09 | 2010-06-09 | Automatic protein crystal high-pass sample observation system |
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| CN201780273U true CN201780273U (en) | 2011-03-30 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019128766A1 (en) * | 2017-12-27 | 2019-07-04 | 大连理工大学 | Experimental system and method applicable to precise regulation of macromolecular crystallization processes |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019128766A1 (en) * | 2017-12-27 | 2019-07-04 | 大连理工大学 | Experimental system and method applicable to precise regulation of macromolecular crystallization processes |
| US11452948B2 (en) | 2017-12-27 | 2022-09-27 | Dalian University Of Technology | Experiment system and method for accurate controlling of macromolecular crystallization process |
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| C14 | Grant of patent or utility model | ||
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110330 Termination date: 20170609 |