CN201553739U - Jet type cell culture bio reaction system - Google Patents
Jet type cell culture bio reaction system Download PDFInfo
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- CN201553739U CN201553739U CN2009202098244U CN200920209824U CN201553739U CN 201553739 U CN201553739 U CN 201553739U CN 2009202098244 U CN2009202098244 U CN 2009202098244U CN 200920209824 U CN200920209824 U CN 200920209824U CN 201553739 U CN201553739 U CN 201553739U
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- liquid
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- reaction container
- type cell
- reaction system
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Abstract
The utility model relates to a jet type cell culture bio reaction system, which comprises a bio reaction container. The system is characterized by further comprising a separation device which is disposed inside the bio reaction container and used for separating cells or cell carriers from liquid , and a liquid extraction device, a changing device and a liquid delivery device which are disposed outside of the bio reaction container. The liquid extraction device is used for extracting liquid separated by the separation device, the changing device is used for receiving the liquid extracted by the liquid extraction device, and the liquid delivery device is used for injecting or jetting the liquid inside the changing device into the bio reaction container, thereby providing power for mixture and change of nutrition compositions inside the bio reaction container. In operation, the system can completely preventing cutting force of mechanical stirring from contacting with bubbles and cells, thereby favoring high-concentration culture of cells.
Description
Technical field
The utility model relates to biotechnology zooblast and culture plant cell device.
Background technology
On the one hand, the cell culture reactor of current home and abroad is the mode that adopts similar microorganism culturing, impels the mixing and the transmission of various nutritive ingredients by mechanical stirring, produces very strong shearing force thus, cultured cells is formed grievous injury, the further raising of restrictive cell density.Particularly in the time will carrying out the cell cultures of higher density, the restriction of the mixing of raising stirring velocity promotion nutritive ingredient and the shearing force pair cell density of transmitting and producing therefrom forms conspicuous contradiction, and the raising productive rate is formed restriction with reducing cost.
On the other hand, the dissolved oxygen of current bio-reactor adopts and directly exchanges in reactor, can produce a large amount of bubbles, and pair cell forms injury, has restricted the further raising of cell density.
On the one hand, the strainer of bio-reactor is subjected to the restriction of stirring velocity, effectively separating liquid and cell or cell carrier again.
Another aspect, current bio-reactor adopts different stirring rake forms to different cell cultures, and versatility is poor.
The utility model content
The purpose of this utility model is to provide a kind of ejection-type cell cultures bio-reaction system, and it has changed the mode of the mixing and the transmission of traditional nutritive ingredient, thereby has solved foregoing problems.
Ejection-type cell cultures bio-reaction system of the present utility model comprises biological reaction container, is characterized in, also comprises: be arranged on the tripping device in the biological reaction container, be used for separating liquid and cell or cell carrier; Be arranged on the outer liquid extracting device of biological reaction container, be used to extract the liquid of separating by tripping device; Be arranged on the outer switch of biological reaction container, be used to receive the liquid that liquid extracting device extracts; And be arranged on the outer liquid transporting apparatus of biological reaction container, be used for the liquid in the switch being injected or spurting into biological reaction container, and the power that nutritive ingredient is mixed, exchanged in the biological reaction container is provided thus.
Described ejection-type cell cultures bio-reaction system, its further characteristics are that switch has gas air intake and outlet port, thereby is configured for liquid is carried out the gas exchange device of gaseous interchange.
Described ejection-type cell cultures bio-reaction system, its further characteristics are that liquid extracting device or liquid transporting apparatus are pneumatic diaphram valves.
Described ejection-type cell cultures bio-reaction system, its further characteristics be, liquid extracting device or e Foerderanlage be adopt flow 10L-100L/ hour can resistant to elevated temperatures pneumatic diaphram valve.
Described ejection-type cell cultures bio-reaction system, its further characteristics are that tripping device is the strainer with stainless steel doline screen cloth of high speed rotating.
Described ejection-type cell cultures bio-reaction system, its further characteristics are that stainless steel mesh has the sieve aperture of 10 μ or 75 μ.
Described ejection-type cell cultures bio-reaction system, its further characteristics are that liquid transporting apparatus injects liquid from the bottom of biological reaction container.
Described ejection-type cell cultures bio-reaction system, its further characteristics are that liquid extracting device extracts the liquid of being separated by tripping device from the top of biological reaction container.
Described ejection-type cell cultures bio-reaction system, its further characteristics are that an end of switch leaves the Liquid extracting interface and nutritive ingredient is replenished interface.
The beneficial effects of the utility model are:
The one, form the mixing and the transmission of various nutritive ingredients by flowing of liquid, eliminate the injury of the formed shearing force of mechanical stirring and its pair cell, help the raising of cell density;
The 2nd, the ventilation in the biological reaction container is placed in the outer switch of biological reaction container carries out, eliminate the injury of bubble pair cell;
The 3rd, can utilize the strainer of high speed rotating to solve separating of cell or cell carrier and liquid, thereby can making full use of centrifugal action, the filtration of high speed rotating makes cell not stick to stop on the screen cloth the obstruction of cell or other material to sieve aperture;
The 4th, versatility is good, goes for suspension culture, microcarrier cultivation and polyester sheet and cultivates; Can improve the density of cell cultures and the productive rate of product greatly thus, reduce facility investment and production cost, bring good economic benefit.
Description of drawings
Fig. 1 is the system architecture synoptic diagram of ejection-type cell cultures bio-reaction system of the present utility model.
Embodiment
As shown in Figure 1, ejection-type cell cultures bio-reaction system comprises biological reaction container 1, tripping device 2, liquid extracting device 3, liquid transporting apparatus 5 and gas exchange device 4.
Biological reaction container 1 is that aspect ratio is 2: 1 a tank body 1.Tripping device 2 is revolving filters, and it is the stainless steel doline screen cloth of 10 μ that this revolving filter adopts sieve aperture.Liquid extracting device 3 and e Foerderanlage 5 adopt flows 10L-100L/ hour can resistant to elevated temperatures pneumatic diaphram valve.Gas exchange device 4 has gas input port 6 and outlet port 7.
Ejection-type cell cultures bio-reaction system comprises that also it does not influence realization of the present utility model as current ripe multipurpose plants of employing such as temperature, PH, dissolved oxygen, sterilizations.
Liquid extracting device 3 extracts liquid to gas exchange device 4 and carries out gaseous interchange from tripping device 2 in operational process, the liquid that is rich in oxygen is injected by the bottom of biological reaction container 1 by liquid transporting apparatus 5 or spurts into biological reaction container 1, and provides therefore that nutritive ingredient is mixed, the power of exchange.Waste gas is discharged from outlet port 7.End at gaseous interchange switch 4 can also leave Liquid extracting interface and the additional interface of nutritive ingredient.The ejection-type biological reaction container is eliminated contacting of churned mechanically shearing force and bubble and cell fully in operational process, help the high-density culture of cell.
Claims (9)
1. an ejection-type cell cultures bio-reaction system comprises biological reaction container, it is characterized in that, also comprises:
Be arranged on the tripping device in the biological reaction container, be used for separating liquid and cell or cell carrier;
Be arranged on the outer liquid extracting device of biological reaction container, be used to extract the liquid of separating by tripping device;
Be arranged on the outer switch of biological reaction container, be used to receive the liquid that liquid extracting device extracts; And
Be arranged on the outer liquid transporting apparatus of biological reaction container, be used for the liquid in the switch being injected or spurting into biological reaction container, and the power that nutritive ingredient is mixed, exchanged in the biological reaction container is provided thus.
2. ejection-type cell cultures bio-reaction system as claimed in claim 1 is characterized in that switch has gas air intake and outlet port, thereby is configured for liquid is carried out the gas exchange device of gaseous interchange.
3. ejection-type cell cultures bio-reaction system as claimed in claim 1 is characterized in that liquid extracting device or liquid transporting apparatus are pneumatic diaphram valves.
4. ejection-type cell cultures bio-reaction system as claimed in claim 1 is characterized in that, liquid extracting device or e Foerderanlage be adopt flow 10L-100L/ hour can resistant to elevated temperatures pneumatic diaphram valve.
5. ejection-type cell cultures bio-reaction system as claimed in claim 1 is characterized in that, tripping device is the strainer with stainless steel doline screen cloth of high speed rotating.
6. ejection-type cell cultures bio-reaction system as claimed in claim 5 is characterized in that stainless steel mesh has the sieve aperture of 10 μ or 75 μ.
7. ejection-type cell cultures bio-reaction system as claimed in claim 1 is characterized in that, liquid transporting apparatus injects liquid from the bottom of biological reaction container.
8. ejection-type cell cultures bio-reaction system as claimed in claim 1 is characterized in that, liquid extracting device extracts the liquid of being separated by tripping device from the top of biological reaction container.
9. ejection-type cell cultures bio-reaction system as claimed in claim 1 is characterized in that, an end of switch leaves the Liquid extracting interface and nutritive ingredient is replenished interface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009202098244U CN201553739U (en) | 2009-09-21 | 2009-09-21 | Jet type cell culture bio reaction system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009202098244U CN201553739U (en) | 2009-09-21 | 2009-09-21 | Jet type cell culture bio reaction system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201553739U true CN201553739U (en) | 2010-08-18 |
Family
ID=42613413
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009202098244U Expired - Fee Related CN201553739U (en) | 2009-09-21 | 2009-09-21 | Jet type cell culture bio reaction system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201553739U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102766575A (en) * | 2011-05-04 | 2012-11-07 | 苏州勃朗科技有限公司 | Animal cell fermentation and separation all-in-one machine |
-
2009
- 2009-09-21 CN CN2009202098244U patent/CN201553739U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102766575A (en) * | 2011-05-04 | 2012-11-07 | 苏州勃朗科技有限公司 | Animal cell fermentation and separation all-in-one machine |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100818 Termination date: 20120921 |