CN201497742U - Immunogold filtration assay kit for combined detection of EA-IgG antibody and VCA-IgA antibody against EB virus - Google Patents
Immunogold filtration assay kit for combined detection of EA-IgG antibody and VCA-IgA antibody against EB virus Download PDFInfo
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- CN201497742U CN201497742U CN2009200916597U CN200920091659U CN201497742U CN 201497742 U CN201497742 U CN 201497742U CN 2009200916597 U CN2009200916597 U CN 2009200916597U CN 200920091659 U CN200920091659 U CN 200920091659U CN 201497742 U CN201497742 U CN 201497742U
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Abstract
The utility model discloses an immunogold filtration assay kit for combined detection of EA(ethylene cellulose)-IgG (immunoglobulin G) antibody and VCA-IgA (immunoglobulin A) antibody against EB ( Epstein-barr) virus. The kit comprises a cassette formed by latching a clamping cover and a clamping seat; a nitrocellulose membrane is fixed in a positioning groove inside the clamping seat; EA antigen, VCA antigen and goat anti-mouse IgG antibody is dotted at the sites with a certain distance on the nitrocellulose membrane; a water-absorbent filter paper is arranged below the nitrocellulose membrane; and a view window is arranged on the clamping cover in the position corresponding to the sites. Based on the principle of immunogold filtration assay, the two kinds of EB virus antigens and the two kinds of EB virus antibodies interact on the same filtration device (i.e. NC membrane) and the color of each dot is observed by naked eyes, thereby achieving the rapid detection of EA-IgG antibody and VCA-IgA antibody against EB virus. The kit has the advantages of simple, rapid and accurate operation, can be applied to the large-scale blood examination, and is helpful for the early diagnosis and screening of patients with nasopharyngeal carcinoma.
Description
Technical field
The utility model relates to biological technical field, especially relates to a kind of Epstein-Barr virus EA-IgG, VCA-IgA antibody joint inspection percolation kit.
Background technology
Nasopharyngeal carcinoma (NPC) is an epithelial cell source place malignant tumour, extensively exists all over the world.In China south, the particularly Delta of the Pearl River and area, Hong Kong, be the district occurred frequently of nasopharyngeal carcinoma in the world.It is the Epstein-Barr virus carrier that China has the health adult more than 80% approximately.In Southeast Asia and north African the higher incidence of disease is arranged also.5 years survival rates can reach 80-90% behind the early stage nasopharyngeal carcinoma radiotherapy, and the survival rate of advanced nasopharyngeal carcinoma (NPC) only is 20%.Therefore strengthen the secondary prevention of nasopharyngeal carcinoma, i.e. discovery early, diagnosis early and early treatment are the keys that improves nasopharyngeal carcinoma patient survival rate.
(Epstein-Barr, EBV) infection is closely related, is an important means that improves NPC patient's survival rate by the antibody early diagnosis NPC that detects EBV for the generation of nasopharyngeal carcinoma and Epstein-Barr virus.
Studies show that, when nasopharyngeal cancer patient average the first three years occurs in clinical symptoms, the content that just can be observed multiple anti EB virus antigen-antibody in its serum significantly raises, thereby the present the most frequently used means of screening for nasopharyngeal cancer patient clinically are that immunoserology detects ebv infection.Wherein the detection index of generally using clinically has EA-IgG, VCA-IgA etc.Because the antibody repertoire of different nasopharyngeal cancer patients is incomplete same, therefore the joint-detection of multiple antigen-antibody can further improve its diagnosis performance.Have experiment to show, the susceptibility of multiple antibody joint inspection and specificity all increase than the inspection of antibody list, and can be used for the risk assessment of nasopharyngeal carcinoma district occurred frequently healthy population examination.
What use clinically at present often is immunofluorescence technique and immune enzyme linked immunosorbent assay to serum Epstein-Barr virus detection of antibodies method.Though immunofluorescence technique detects EB antibody sensitivity and specificity is better, but its result's judgement has subjectivity, is difficult for standardization.ELISA and fluorescence method are more quick and convenient, and are easy to standardization, but consuming time longer.
How efficient fast the antibody of multiple Epstein-Barr virus is detected, be a focus of research at present.The colloidal gold immunity percolation method is arisen at the historic moment on the ELISA basis, its principle is to be carrier with the nitrocellulose filter, it is simply quick that the method detects antibody, do not need other instrument and equipments, a few minutes the testing result that can detect by an unaided eye, in clinical detection, obtained at present widely and used, and in whole immunology detection scope, also be widely used.Adopt the colloidal gold immunity percolation technical tie-up to detect Epstein-Barr virus EA-IgG, VCA-IgA antibody, have very high practicality to detect meaning clinically, but be applied to the antibody combined detection kit of Epstein-Barr virus at present as yet.
Summary of the invention
The purpose of this utility model is to provide a kind of Epstein-Barr virus EA-IgG, VCA-IgA antibody joint inspection percolation kit.
For achieving the above object, the utility model can be taked following technical proposals:
Epstein-Barr virus EA-IgG described in the utility model, VCA-IgA antibody joint inspection percolation kit, comprise box body, described box body is fastened by Ka Gai and deck and forms, be fixed with nitrocellulose filter in the locating slot in described deck, put EA, VCA antigen and the sheep anti-mouse igg antibody that is shaped on Epstein-Barr virus on the site that is provided with at interval thereon, below described nitrocellulose filter, be lined with absorbent filter, offer watch window covering with the corresponding card in described site.
Described box body be shaped as rectangle, triangle or square, be opened in watch window that card covers for circular or square.
Advantage of the present utility model is to have utilized the colloidal gold immunity percolation ratio juris, the two kinds of antigens, antibody that make Epstein-Barr virus are at same percolating device (nitrocellulose filter, be the NC film) on react, wash, and the colour developing situation by each spot of visual inspection, thereby EA-IgG, the VCA-IgA antibody of Epstein-Barr virus is carried out fast detecting; Easy and simple to handle, rapid, accurate, applicable to extensive blood test, more help nasopharyngeal carcinoma patient's early detection and diagnosis.
The utility model kit is compared with the ELISA kit, and its advantage is:
1, the joint-detection that detects two kinds of antibody of multiple antibody Epstein-Barr virus has simultaneously improved the sensitivity and the specificity that detect, and the diagnosis of nasopharyngeal carcinoma is had the good clinical meaning.
2, short whole experiment of reaction time can be finished in 3-5 minute, was fit to large-scale blood examination fast; It is few that this kit detects step, but susceptibility is similar with the ELISA that finished in need 1-2 hour;
3, do not need other instrument and equipments in the testing process, so testing result is not subjected to the influence of instrument yet, is not subjected to the influence of reaction environment simultaneously, adding the colloid gold label thing can develop the color, easy and simple to handle; 4, required detection specimen amount is little, and the blood flow volume that needs is 30 μ l only, only gathers finger tip blood or ear blood gets final product; And index of ELISA detection needs 100 μ l serum, and blood sampling volume is big.
The utility model kit is compared with traditional colloidal gold kit, and its advantage is:
1, detect two kinds of antigens of Epstein-Barr virus simultaneously, all single inspection of sensitivity and specificity is high;
2, profile is changeable and attractive in appearance, and is easy to use, is fit to the self-service detection of family, finds and diagnosis early the morning that helps the nasopharyngeal carcinoma illness.
Description of drawings
Fig. 1, Fig. 2, Fig. 3 are box body structure synoptic diagram of the present utility model.
Embodiment
As Fig. 1,2, shown in 3, described Epstein-Barr virus EA-IgG, VCA-IgA antibody joint inspection percolation kit, comprise box body 1, described box body 1 is fastened by Ka Gai and deck and forms, be fixed with nitrocellulose filter 2 in the locating slot in described deck, on the site 3 of certain distance, put the EA that is shaped on Epstein-Barr virus thereon, VCA antigen and sheep anti-mouse igg antibody, below described nitrocellulose filter 2, be lined with absorbent filter, offer the watch window 4 of area covering, can make nitrocellulose filter 2 and filter paper fixing firm in locating slot like this less than the locating slot area with described site 3 corresponding cards; For making things convenient for family to use, the shape of described box body 1 can be rectangle, triangle, square or some other geometric configuration, is opened in the watch window 4 that card covers and also can be other geometric configuratioies such as circular, quincunx or square.
Kit test method of the present invention is as follows:
1, kit is taken out horizontal positioned, room temperature (18～25 ℃) balance 30 minutes from cold storage environment;
2, each device is gone up and is added sample to be tested 30 μ l;
3, treat that sample is blotted the back and adds 6 of damping fluids (300ul);
4, add gold mark 2 of liquid (100ul);
5, treat that liquid is blotted the back and adds 6 of damping fluids;
6, treat on the film that liquid blots back 5min visual inspection result, Quality Control point shows punctation, and there is the spot of redfree observation respective detection position, punctation occurs and is judged to this sample Epstein-Barr virus antibody and is positive in vivo, and the redfree spot promptly is judged to feminine gender.
Claims (2)
1. an Epstein-Barr virus EA-IgG, VCA-IgA antibody joint inspection percolation kit, comprise box body (1), it is characterized in that: described box body (1) is fastened by Ka Gai and deck and forms, be fixed with nitrocellulose filter (2) in the locating slot in described deck, EA, VCA antigen and the sheep anti-mouse igg antibody that point is shaped on Epstein-Barr virus gone up in the site (3) that is provided with at interval thereon, below at described nitrocellulose filter (2) is lined with absorbent filter, offers watch window (4) covering with the corresponding card in described site (3).
2. Epstein-Barr virus EA-IgG according to claim 1, VCA-IgA antibody joint inspection percolation kit is characterized in that: described box body (1) be shaped as rectangle, triangle or square, be opened in watch window (4) that card covers for circular or square.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200916597U CN201497742U (en) | 2009-07-17 | 2009-07-17 | Immunogold filtration assay kit for combined detection of EA-IgG antibody and VCA-IgA antibody against EB virus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200916597U CN201497742U (en) | 2009-07-17 | 2009-07-17 | Immunogold filtration assay kit for combined detection of EA-IgG antibody and VCA-IgA antibody against EB virus |
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| Publication Number | Publication Date |
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| CN201497742U true CN201497742U (en) | 2010-06-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2009200916597U Expired - Lifetime CN201497742U (en) | 2009-07-17 | 2009-07-17 | Immunogold filtration assay kit for combined detection of EA-IgG antibody and VCA-IgA antibody against EB virus |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014118151A1 (en) * | 2013-02-01 | 2014-08-07 | Euroimmun Medizinische Labordiagnostika Ag | Method and apparatus for diagnosing a nasopharyngeal carcinoma |
| CN105785040A (en) * | 2016-03-31 | 2016-07-20 | 新疆农业大学 | EHV-1 serum antibody detection kit as well as preparation method and application |
| CN110376373A (en) * | 2019-08-23 | 2019-10-25 | 王爱芳 | A kind of colloidal gold accelerates reaction unit and detection method |
-
2009
- 2009-07-17 CN CN2009200916597U patent/CN201497742U/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014118151A1 (en) * | 2013-02-01 | 2014-08-07 | Euroimmun Medizinische Labordiagnostika Ag | Method and apparatus for diagnosing a nasopharyngeal carcinoma |
| CN105308459A (en) * | 2013-02-01 | 2016-02-03 | 欧蒙医学诊断技术有限公司 | Method and apparatus for diagnosing a nasopharyngeal carcinoma |
| CN105785040A (en) * | 2016-03-31 | 2016-07-20 | 新疆农业大学 | EHV-1 serum antibody detection kit as well as preparation method and application |
| CN105785040B (en) * | 2016-03-31 | 2018-05-25 | 新疆农业大学 | A kind of EHV-1 Serum Antibody Detections kit and preparation method and application |
| CN110376373A (en) * | 2019-08-23 | 2019-10-25 | 王爱芳 | A kind of colloidal gold accelerates reaction unit and detection method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: ZHENGZHOU AUTOBIO ENGINEERING CO., LTD. Free format text: FORMER NAME: ZHENGZHOU AUTOBIO DIAGNOSTICS CO., LTD. |
|
| CP03 | Change of name, title or address |
Address after: 450016 Zhengzhou economic and Technological Development Zone, Henan, North Road, No. 1, No. 87 Patentee after: Autobio Diagnostics Co., Ltd. Address before: 450016, No. fifth, No. 87, No. 1, main street, Zhengzhou economic and Technological Development Zone, Henan Patentee before: Zhengzhou Autobio Diagnostics Co., Ltd. |
|
| CX01 | Expiry of patent term |
Granted publication date: 20100602 |
|
| CX01 | Expiry of patent term |