Test piece is detected in the microchannel
Technical field
The utility model is that test piece is detected in a kind of microchannel, and it detects test piece especially in regard to a kind of a kind of microchannel that at least one flow-guiding channel is set in each capillary fluid channel.
Background technology
Development along with Measurement for Biotechnique, some need particular place such as hospital, control laboratory, laboratory originally, and the Interventions Requested that could obtain by exact instrument or the medical professionalism talent such as doctor, docimaster, researcher, as biochemistry, immunity, gene analysis etc., not only can carry out at present in the clinic, even patient also can detect at home voluntarily, this technology be called as on-the-spot test in time (Point of care test, POCT).Common POCT biochemical investigation project has blood sugar, cholesterol, uric acid, and the immunity inspection project then has pregnancy, drug abuse, cancer index, glycated hemoglobin and enterovirus gene etc.This technology and the commodity of deriving thereof can not only be applied to biomedicine, more can be applicable to animal doctor, agricultural, environmental protection and industrial aspect.
The POCT that generally uses detects test piece in the market, after sample splashes into the detection test piece, its subject matter to be measured can produce chemical reaction with the suction area or the material of detection zone, cause the variations such as optics, the quantity of electric charge of suction area or detection zone, utilize pick-up unit to measure union again and learn subject matter concentration to be measured.
The Electrochemical Detection test piece of prior art as shown in Figure 1, Electrochemical Detection zone 12 and conducting channel are arranged on its substrate 10, conducting channel comprises working electrode 13, counter electrode 14, and Electrochemical Detection zone 12 is connected with working electrode 13, counter electrode 14 respectively, and working electrode 13 and counter electrode 14 are connected with first connector 18 and second connector 19 by first lead 15 and second lead 16 respectively.One feeder connection 17 is still arranged on the substrate, be connected in an end of feeder connection and the Electrochemical Detection zone, and all covered by a upper cover plate 11.After sample splashes into feeder connection 17, feeder connection 17 can utilize capillarity to carry subject matter to be measured and be diffused into Electrochemical Detection zone 12, subject matter to be measured can and produce chemical change with the substance reaction in Electrochemical Detection zone 12, the signal that its quantity of electric charge changes can be sent to pick-up unit (figure does not show) via electrode, utilizes pick-up unit to measure union again and can learn subject matter concentration to be measured.
So, static discharge (Electrostatic Discharge; ESD) be to cause most electronic component or electronic system to be subjected to the excessively electrically principal element of stress rupture.This destruction can cause semiconductor element, forms a kind of temporary or nonvolatil breaking-up, thereby influences the circuit function of IC, and makes that electronic product work is undesired.And mostly the generation that static discharge destroys is because human factor forms, and is difficult to again avoid.For avoiding pick-up unit to suffer the problem of ESD, above-mentioned prior art, Electrochemical Detection zone 12 set working electrode a end a, working electrode b end b must maintain a certain distance with the both sides of the edge of test piece, otherwise static will be easy to conduct to the circuit of pick-up unit from feeder connection 17 via working electrode a end a, working electrode b end b, causes the machine of working as of pick-up unit.But if when electrode working electrode a end a, working electrode b end b is increased apart from the test piece edge, though reduced the probability that ESD destroys, reduced the capillarity of feeder connection 17 also and made the normal situation that suction takes place to be difficult for of sample, cause the problem of user on product uses, this Electrochemical Detection test piece can't correctly be used.
For this reason, the utility model proposes a kind of microchannel and detect test piece, not only can avoid the interference problem of ESD but also can effectively promote the capillarity that sample sucks, to improve above-mentioned disappearance.
Summary of the invention
Fundamental purpose of the present utility model is providing a kind of microchannel to detect test piece, and it can effectively solve the ESD protection question that prior art is produced when not enough because of the distance at working electrode a end, working electrode b end and the edge of test piece.
Another purpose of the present utility model is providing a kind of microchannel to detect test piece, and it is provided with at least one flow-guiding channel in each capillary fluid channel, can strengthen the capillarity of the capillary fluid channel of prior art.
The utility model provides a kind of microchannel to detect test piece, and it comprises a substrate, and substrate is provided with Electrochemical Detection zone and conducting channel.Conducting channel comprises working electrode and counter electrode, and working electrode and counter electrode are connected with Electrochemical Detection zone respectively, and working electrode and counter electrode pass through first lead and second lead respectively and be connected with first connector and second connector.One feeder connection is arranged on the substrate in addition, be connected in an end of feeder connection and the Electrochemical Detection zone, and this Electrochemical Detection zone is all covered by a upper cover plate with this feeder connection.And upper cover plate and this substrate manifold card mutually comprise at least one flow-guiding channel so that the feeder connection place forms a plurality of capillary fluid channel in each capillary fluid channel, and these flow-guiding channels can be strengthened the capillarity of this capillary fluid channel.
By said structure, the utility model can effectively solve prior art because of the ESD protection question that the distance at working electrode a end, working electrode b end and the edge of test piece is produced when not enough, has reached beneficial technical effects.
Beneath by the appended graphic explanation in detail of specific embodiment cooperation, when the effect that is easier to understand the purpose of this utility model, technology contents, characteristics and is reached.
Description of drawings
Fig. 1 is the structural representation of the Electrochemical Detection test piece of prior art;
Fig. 2 is the structural representation that test piece is detected in the microchannel of an embodiment of the present utility model;
Fig. 3 is vertical view and the upward view that test piece is detected in the microchannel of an embodiment of the present utility model;
Fig. 4 is vertical view and the upward view that test piece is detected in the microchannel of another embodiment of the present utility model.
Description of reference numerals
The 10-substrate; The 11-upper cover plate; 12-Electrochemical Detection zone; The 13-working electrode; The 14-counter electrode; 15-first lead; 16-second lead; The 17-feeder connection; 18-first connector; 19-second connector; A-working electrode a end; B-working electrode b end; The 20-substrate; The 22-upper cover plate; 28-Electrochemical Detection zone; The 30-counter electrode; The 32-working electrode; The 36-feeder connection; 38-capillary fluid channel; The 40-flow-guiding channel; 42-first lead; 44-second lead; 46-first connector; 48-second connector.
Embodiment
Detect test piece for describing microchannel of the present utility model in detail, can be used for various POCT analytical equipments, as blood-sugar detecting instrument, cholesterol detection instrument, uric acid detector and lactate detection instrument etc.First embodiment of the present utility model please refer to Fig. 2~Fig. 3.A kind of microchannel of the present utility model is detected test piece and is comprised a substrate 20 and a upper cover plate 22.One Electrochemical Detection zone 28 and conducting channel are arranged on substrate 20, and conducting channel comprises counter electrode 30 and working electrode 32, and 32 being connected with Electrochemical Detection zone 28 respectively of counter electrode 30 and working electrode.And working electrode 32 and counter electrode 30 are connected with first connector 46 and second connector 48 by first lead 42 and second lead 44 respectively.Wherein, working electrode 32 refers to a kind of when existing or not having electron transport reagent, can carry out the electrode of electrooxidation or electroreduction to analyte (subject matter to be measured).Counter electrode 30 refers to a kind of electrodes that use in pairs with working electrode 32, equates with amount of current by working electrode 32 but direction is opposite that by the electrochemical source of current on it electrode can adopt any conductive material making, as palladium, platinum, gold, silver and carbon.First connector 46 and second connector 48 are positioned at the edge of substrate 20, are used for being connected with a pick-up unit (figure does not show).Pick-up unit can be used to detect at least one electrical parameter (as, subject matter concentration to be measured).
Feeder connection 36 is positioned on the substrate 20, is connected in an end of feeder connection 36 and the Electrochemical Detection zone 28, and is all covered by upper cover plate 22.Form a plurality of capillary fluid channel 38 at feeder connection 36 places, comprise at least one flow-guiding channel 40 in each capillary fluid channel 38, these flow-guiding channels 40 can be strengthened the capillarity of this capillary fluid channel, and the distance that two adjacent flow-guiding channels are 40 is between 0.2 millimeter (mm)~1 millimeter (mm).Wherein, utilize screen painting that all flow-guiding channels 40 are printed in the feeder connection 36, stick a upper cover plate 22 afterwards, then form capillary fluid channel 38.
Behind the subject matter admission passage to be measured inlet 36, substance reaction that can be by capillary fluid channel 38 and these flow-guiding channels 40 and Electrochemical Detection zone 28 also produces chemical change.After producing chemical change, the signal that its quantity of electric charge changes can be sent to pick-up unit via first connector 46 and second connector 48, utilize pick-up unit received signal union to learn subject matter concentration to be measured again, the utility model is the ESD protection question that is produced when utilizing the design of flow-guiding channel 40 effectively to solve prior art because of the distance deficiency at working electrode a end a, working electrode b end b and the edge of test piece.Wherein, pick-up unit is a kind of POCT pick-up unit, for example blood-sugar detecting instrument, cholesterol detection instrument, uric acid detector and lactate detection instrument one of them.
Go on to say another kind of embodiment of the present utility model, please refer to Fig. 4, a present embodiment and a last embodiment difference are, feeder connection 36 is at the front end of substrate 20 among Fig. 4, the feeder connection of feeder connection 36 then is positioned at the both sides of substrate 20 among Fig. 3, the design of two embodiment is the same, so all the other all are identical partly.
More than be illustrative to description of the present utility model; and it is nonrestrictive; those skilled in the art is understood, and can carry out many modifications, variation or equivalence to it within spirit that claim limits and scope, but they will fall in the protection domain of the present utility model all.