CN201464365U - Micro channel control device for surface plasma resonance bioanalyzer - Google Patents
Micro channel control device for surface plasma resonance bioanalyzer Download PDFInfo
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- CN201464365U CN201464365U CN 200920088096 CN200920088096U CN201464365U CN 201464365 U CN201464365 U CN 201464365U CN 200920088096 CN200920088096 CN 200920088096 CN 200920088096 U CN200920088096 U CN 200920088096U CN 201464365 U CN201464365 U CN 201464365U
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Abstract
The utility model discloses a micro channel control device for a surface plasma resonance bioanalyzer, comprising a micro solenoid valve, a micro three way cock, a peristaltic pump, a two-position three-way switch valve, a two-position twelve-way micro sample valve, a sample loop, a microflow tank, samples, buffer solution, regenerative solution and a detection device. Switching between series connection and parallel connection of three flow channels of the microflow tank can be realized by opening and closing the micro solenoid valve, the variation of positions of the two-position twelve-way micro sample valve and the selection of the sample loop according to different requirements; and conversion between the buffer solution and the regenerative solution can be realized by switching between two positions of the two-position three-way switch valve; thus, the regeneration of a biomolecule recognizable membrane is realized. The application of the device can greatly increase the analysis efficiency of the surface plasma resonance bioanalyzer, improve the sensitivity thereof, reduce sample consumption, shorten the time of analysis and test and realize accurate quantitative analysis and the regeneration of the biomolecule recognizable membrane.
Description
Technical field
The utility model relates to optical surface plasma resonance (Surface plasmon resonance, SPR) sensing technology, biosensor technique, microflow control technique, fluid channel process technology and accurate quantification analytical technology, particularly by little valve and Micropump are installed on the pipeline connecting parts of microfluid pool, the quick switching between three passages of realization microfluid pool and the accurate quantification of biological sample detect.
Background technology
For biological analyser based on SPR, if can be according to actual conditions, select the different number of active lanes and the consumption of sample, not only can greatly improve the detection efficiency of instrument, save the test duration, but also can reduce sample consumption, and be a kind of measure that effectively utilizes sample, significant in bioanalysis.
At present, though the SPR biological analyser is highly sensitive with it, fast, need not mark etc. characteristics, caused people's extensive concern, and very vast market prospect arranged, but because the cost of SPR biological analyser is higher, usually need units up to a million, promote the use of thereby be difficult to large tracts of land, domestic only have several institutes Scientific Research in University Laboratory few in number to have.The fluid channel control system is the core of SPR biological analyser, the sealing quality of flow cell, and the control of sample feeding system and the switching between the passage directly influence the performance and the instrument cost of SPR biological analyser.
Problem that a kind of fluid channel control device of surface plasma resonance biological analyser has solved that connection in series-parallel between the hyperchannel is switched and the multiple one-tenth of realizing detecting simultaneously a kind of sample grades, thereby the whole processing cost of lowering apparatus, improve detection efficiency, have very great practical significance.
The utility model content
In order to improve the analytical test efficient of SPR biological analyser, reduce sample consumption, realization detects the accurate quantification of sample, simultaneously, according to different demands, the connection in series-parallel that realizes detection system is switched fast, and the utility model provides a kind of fluid channel control device of surface plasma resonance biological analyser.
The technical scheme that technical matters adopted that the utility model solves is:
1. surface plasma resonance biological analyser fluid channel control device, it comprises two 12 logical miniature sampling valves (13), sample loop (15), microfluid pool (26) and passages (34,35,36), one end of passage (34) and passage (35) is connected with miniature electromagnetic valve (29,30,32) with three-way connection (27) by miniature three-way joint (33) respectively, miniature electromagnetic valve (30) is connected with peristaltic pump (31) respectively with miniature electromagnetic valve (32) then, and an end of passage (36) directly is connected with peristaltic pump (31); The other end of passage (34) is connected with the port (11) of two 12 logical miniature sampling valves (13), and the another port of passage (35) and passage (36) is connected with miniature electromagnetic valve (21,23,25) with miniature three-way joint (24) by miniature three-way joint (22) respectively, and miniature electromagnetic valve (21) is connected with damping fluid (28) with the port (5) of two 12 logical miniature sampling valves (13) respectively again with miniature electromagnetic valve (25); The port (4) of two 12 logical miniature sampling valves (13) is connected with two-bit triplet transfer valve (20) with two-bit triplet transfer valve (16) respectively with port (10).
2. microfluid pool (26) is made up of 3 parallel passages (34), passage (35) and passage (36).
3. adopt two 12 logical miniature sampling valves of accurate quantification, as shown in Figure 1, make it with three microchannels of SPR biological analyser fluid channel reaction tank in two be connected (Fig. 2, Fig. 3), another passage is as reference channel, thereby can realize the parallel function of SPR biological analyser; In addition, adopt sample loop to realize that accurate quantification detects, and strengthens the sealing property of microchannel.
4. adopt the fluid channel control system, the fluid channel control system is made up of multiple micro electromagnetic valve and three-way connection, the series connection and the parallel connection that alternately open and close three passages realizing SPR biological analyser microfluid pool by two 12 logical miniature sampling valves realize detecting simultaneously three kinds of heterogeneities in a kind of sample.
5. adopt biomolecule identification film regenerative system, by the two-bit triplet transfer valve, the quick switching of control damping fluid and regenerated liquid, thereby the regeneration of realization biomolecule identification film.Miniature electromagnetic valve and miniature three-way joint are housed on passage, its two ends are connected with the buffering liquid phase with the port of peristaltic pump respectively, the port of two 12 logical miniature sampling valves is connected with the two-bit triplet transfer valve respectively, and the two-bit triplet transfer valve is being controlled the mutual conversion between damping fluid and the regenerated liquid.
The beneficial effect that the utility model had is:
1. dispose the multithread road sampling system of carry sample ring, both can realize that accurate quantification detected, can detect several samples simultaneously again, increased substantially the detection efficiency of SPR biological analyser, reduced sample consumption simultaneously.
2. be provided with biomolecule identification membrane regeneration plant, can realize the regeneration of biomolecule identification film, shorten detection time, reduce cost.
3. have exercisable little valve control system,, can realize that the connection in series-parallel of detection system is switched fast, greatly improved detection efficiency according to different demands.
Description of drawings
Fig. 1 is a kind of surface plasma resonance biological analyser fluid channel switching construction figure.
Fig. 2 is a kind of surface plasma resonance biological analyser fluid channel control device front view.
Fig. 3 is a kind of surface plasma resonance biological analyser fluid channel control device vertical view.
Embodiment
Embodiment: as among Fig. 1 being a kind of surface plasma resonance biological analyser fluid channel control device.1,2,3,4,5,6,7,8,9,10,11,12 is ports of two 12 logical miniature sampling valves 13 among Fig. 1, the 14th, and sample, the 15th, sample loop, 17, the 28th, damping fluid, the 18th, regenerated liquid, the 19th, waste liquid, 16, the 20th, the two-bit triplet transfer valve, 21, the 23,25,29,30, the 32nd, miniature electromagnetic valve, the 22,24,27, the 33rd, miniature three-way joint, the 26th, microfluid pool, 34, the 35, the 36th, three passages of microfluid pool, the 31st, peristaltic pump.
37 is sample introduction runners among Fig. 2, the 38th, go out the sample runner, and the 39th, bio-sensitive film, the 40th, prism, the 41st, ccd array, the 42nd, incident beam.
34,35 and 36 is respectively three passages of same microfluid pool of flowing through among Fig. 3.
Embodiment when 1, sample is in parallel in the realization microfluid pool comprises sampling, sample introduction and three processes of biomolecule identification film regeneration:
1) in the sampling process, miniature electromagnetic valve 21,25,29,32 is opened, 23,30 close, peristaltic pump 31 extracts two kinds of different sample 1-14,2-14 and flows into sample loop 1-15,2-15 respectively, when sample is full of the whole sample ring, unnecessary sample flows out through two waste liquid outlets 8,9 of two 12 logical miniature sampling valves 13, damping fluid 1-17,2-17, directly enter two passages 34,35 of microfluid pool 26 through the port of two 12 logical miniature sampling valves respectively, damping fluid 28 flows directly into another passage 36 of microfluid pool 26 and finishes sampling process.
2) in the sample introduction process, miniature electromagnetic valve 21,25,29,32 is opened, 23,30 close, by switching the position of two 12 logical miniature sampling valves 13, peristaltic pump 31 extracts buffer solution 1-17,2-17, make it flow into sample loop 1-15,2-15 earlier, the sample that promotes among sample loop 1-15, the 2-15 with buffer solution 1-17,2-17 enters two different passages 34,35. of microfluid pool 26 respectively when the incident light velocity 42 shines the surface-sensitive rete 39 of prism 40, produce surface plasma resonance, resonance signal is detected by ccd array 41.
3) in the biomolecule identification film regenerative process,, make between buffer solution 1-17,2-17 and actified solution 1-18, the 2-18 and change, realize the regeneration of biomolecule identification film by switching the position of two-bit triplet transfer valve 16,20.
Embodiment when 2, sample is connected in the realization microfluid pool comprises sampling, sample introduction and three processes of biomolecule identification film regeneration:
1) in the sampling process, miniature electromagnetic valve 21,25,29,32 is closed, 23,30 open, peristaltic pump 31 draw samples 1-14 flow into sample loop 1-15, when sample is full of the whole sample ring, unnecessary sample flows out through two 12 logical miniature sampling valve 13 waste liquid outlets 8, and damping fluid 1-17 finishes sampling process through three passages 34,35,36 that the port of two 12 logical miniature sampling valves 13 directly enters microfluid pool 26.
2) in the sample introduction process, miniature electromagnetic valve 21,25,29,32 is closed, 23,30 open, by switching the position of two 12 logical miniature sampling valves 13, peristaltic pump 31 extracts buffer solution 1-17, make it flow into sample loop 1-15, enter three different passages of microfluid pool 26 with the sample 1-14 among the buffer solution 1-17 promotion sample loop 1-15.When the incident light velocity 42 shines the surface-sensitive rete 39 of prism 40, produce surface plasma resonance, resonance signal is detected by ccd array 41.
3) in the biomolecule identification film regenerative process,, make buffer solution 1-17 and actified solution, change between the 1-18, realize the regeneration of biomolecule identification film by switching the position of two-bit triplet transfer valve 16.
Claims (2)
1. surface plasma resonance biological analyser fluid channel control device, it is characterized in that: it comprises two 12 logical miniature sampling valve (13), sample loop (15) and microfluid pools (26), one end of passage of microfluid pool (34) and passage (35) is connected with miniature electromagnetic valve (29,30,32) with (27) by miniature three-way joint (33) respectively, miniature electromagnetic valve (30) is connected with peristaltic pump (31) respectively with (32) then, and an end of passage (36) directly is connected with peristaltic pump (31); The other end of passage (34) is connected with the port (11) of two 12 logical miniature sampling valves (13), and the another port of passage (35) and passage (36) is connected with miniature electromagnetic valve (21,23,25) with (24) by miniature three-way joint (22) respectively, and miniature electromagnetic valve (21) is connected with damping fluid (28) with the port (5) of two 12 logical miniature sampling valves (13) respectively again with (25); The port (4) of two 12 logical miniature sampling valves (13) is connected with two-bit triplet transfer valve (20) with two-bit triplet transfer valve (16) respectively with port (10).
2. the fluid channel control device of a kind of surface plasma resonance biological analyser according to claim 1 is characterized in that: microfluid pool (26) is made up of 3 parallel passages (34), passage (35) and passage (36).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200920088096 CN201464365U (en) | 2009-01-12 | 2009-01-12 | Micro channel control device for surface plasma resonance bioanalyzer |
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| CN 200920088096 CN201464365U (en) | 2009-01-12 | 2009-01-12 | Micro channel control device for surface plasma resonance bioanalyzer |
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| CN 200920088096 Expired - Fee Related CN201464365U (en) | 2009-01-12 | 2009-01-12 | Micro channel control device for surface plasma resonance bioanalyzer |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102636461A (en) * | 2012-04-17 | 2012-08-15 | 王利兵 | Surface plasmon resonance spectrometer with embedded sample purifying module and detection method thereof |
| WO2012130188A3 (en) * | 2011-03-30 | 2012-11-22 | 深圳市麦迪聪医疗电子有限公司 | Flow-type multi-channel biochemical analyzer |
| CN105170204A (en) * | 2015-08-25 | 2015-12-23 | 辽宁中医药大学 | Liquid continuous switching structure and micro fluidic chip comprising same |
| CN113484283A (en) * | 2021-05-19 | 2021-10-08 | 南京医科大学第二附属医院 | Detection system and method for simultaneously measuring multiple biological information |
-
2009
- 2009-01-12 CN CN 200920088096 patent/CN201464365U/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012130188A3 (en) * | 2011-03-30 | 2012-11-22 | 深圳市麦迪聪医疗电子有限公司 | Flow-type multi-channel biochemical analyzer |
| CN102636461A (en) * | 2012-04-17 | 2012-08-15 | 王利兵 | Surface plasmon resonance spectrometer with embedded sample purifying module and detection method thereof |
| CN102636461B (en) * | 2012-04-17 | 2014-08-27 | 王利兵 | Surface plasmon resonance spectrometer with embedded sample purifying module and detection method thereof |
| CN105170204A (en) * | 2015-08-25 | 2015-12-23 | 辽宁中医药大学 | Liquid continuous switching structure and micro fluidic chip comprising same |
| CN105170204B (en) * | 2015-08-25 | 2017-01-18 | 辽宁中医药大学 | Liquid continuous switching structure and micro fluidic chip comprising same |
| CN113484283A (en) * | 2021-05-19 | 2021-10-08 | 南京医科大学第二附属医院 | Detection system and method for simultaneously measuring multiple biological information |
| CN113484283B (en) * | 2021-05-19 | 2024-02-06 | 南京医科大学第二附属医院 | Detection system and method for simultaneously measuring multiple biological information |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100512 Termination date: 20110112 |