CN201459107U

CN201459107U - Liquid flow micro-environment simulator formed by bacterial biofilms

Info

Publication number: CN201459107U
Application number: CN 200920128480
Authority: CN
Inventors: 余加林; 邹志慧; 李芳�
Original assignee: Childrens Hospital of Chongqing Medical University
Current assignee: Childrens Hospital of Chongqing Medical University
Priority date: 2009-08-14
Filing date: 2009-08-14
Publication date: 2010-05-12
Anticipated expiration: 2019-08-14

Abstract

The utility model provides a liquid flow micro-environment simulator formed by bacterial BF, which comprises a constant-temperature shaker (1), a container (2) and a BF carrier (3), wherein the container for containing substances is arranged on the constant-temperature shaker (1), and the BF carrier (3) is fixed on the bottom portion of the container (2). The liquid flow micro-environment simulator can effectively simulate various micro-environments of blood and urine in organisms, and leads the bacterial BF to be formed in environments extremely similar to the micro-environments. Simultaneously, the liquid flow micro-environment simulator is simple in structure, high in manufacturing success ratio and convenient for viewing and operation, and provides reliable bacterial BF sources for further developing medicine and screening of the medicine of relative bacterial BF in built-in pipes in blood systems and urine systems.

Description

The liquid stream microenvironment simulator that a kind of bacterial biofilm forms

Technical field

The utility model relates to the microenvironment simulator that a kind of bacterial biofilm (BF) forms, and particularly relates to a kind of microenvironment simulator about liquid flowing states such as human recycle system, urinary systems, belongs to medical science model field.

Background technology

Along with the continuous progress of medical skill, more and more artificial built-in medical apparatus are applied to clinical, as technology such as trachea cannula, endovascular prosthesis, catheter, joint prosthesis and artificial cardiac valve prosthesises.These devices it is found that benefiting the patient simultaneously bacterium is a carrier with these built-in materials often, cause infection to be difficult to remove in its surperficial field planting and then formation bacterium BF, so that infect protracted course of disease even threat to life.Therefore, BF studies over nearly 20 years and comes into one's own gradually.Bacterium BF is a kind of biology of bacteria characteristic, be that bacterium is in process of growth, for adapt to that living environment is adsorbed in that inertia or surface of active material form a kind of with swim the corresponding growth pattern of cell, the bacterium of forming by bacterium and self the excretory extracellular matrix microenvironment of living.Discover that this bacterioid colony is different at morphological structure and biochemical characteristic with the bacterium that swims, resistance is extremely strong, can escape host immune effect and microbiotic and kill and wound, and infection site is difficult to thorough removing, causes infecting protracted course of disease.Therefore, further the medicine of the interior dependency bacterial biofilm of the anti-built-in conduit of exploitation and drug screening, therapeutic evaluation are to need the urgent problem that solves clinically.

After the nineties in last century, along with the development of related discipline and to the BF bacterium in the understanding of importance medically, BF research is developed rapidly.The method of laboratory in vitro study bacterium BF mainly contains plating method, transparent embrane method and continuous culture method etc. now.Plating method is a static cultivation, and is different with the microenvironment of systemic circulatory system, urinary system liquid-flow.Though and multiple factors such as transparent embrane method and the continuous culture method interior BF development of analogue body preferably desired nutritional desired substance, temperature, time need special bactogen, requirement of experiment condition height has limited its widespread use.

The utility model content

The utility model purpose is to set up a kind of simplely, low to investigator's technical requirements, and Modelling success ratio height more can be simulated the microenvironment of uropoiesis, recycle system liquid-flow, is convenient to the liquid stream microenvironment simulator that the bacterium BF of later observation research forms.

The purpose of this utility model is achieved in that the liquid stream microenvironment simulator that a kind of bacterium BF forms, it is characterized in that: this simulator comprises the constant temperature shaking table, place the container that holds material in the described constant temperature shaking table, and the BF carrier that is fixed in described container bottom.

Above-mentioned BF carrier is the biofilm load carrier of simulated blood vessel wall or urinary catheter wall; In order to study conveniently, once use this simulator to obtain many group experimental datas, described BF carrier series connection can be fixed on the slide glass, be fixed in described container bottom again.

Said vesse is an open containers, and when using this simulator, the material that described container contents is received is a bacterium liquid; The placed in-line BF carrier that is fixed on the slide glass places the interior bacterium liquid of container, and is fixed in container bottom.

Above-mentioned constant temperature shaking table provides isoperibol and the generation and the approaching bacterium flow velocity of body fluid flow velocity of bacterial growth, with the intravital recycle system of anthropomorphic dummy or urinary system microenvironment.

The beneficial effects of the utility model are: because the utility model adopts the constant temperature shaking table that constant temperature is provided and shakes, make bacterium liquid in the container in the temperature constant state current downflow, simultaneously this simulator has utilized the placed in-line biofilm load carrier of fine rule that passes through that is fixed on container bottom to form when shaking table rotates and breathe heavily stream, produce shearing force, thereby at blood in the body, urine is in the flow velocity difference at each position, change the flow velocity of bacterium liquid by the rotating speed that changes shaking table, realize imitating better organismic internal environment, for further developing blood system, the medicine of urinary system built-in pipe dependency bacterium BF and the screening of medicine thereof provide reliable bacterium BF the source. simultaneously, the utility model simulator is simple in structure, Modelling success ratio height, be convenient to observe, easy to operate.

Description of drawings

Fig. 1 is the structural representation of the utility model embodiment.

Fig. 2 is the structure of container synoptic diagram.

Embodiment

Below in conjunction with the drawings and specific embodiments the utility model is described in further detail.

Embodiment: from Fig. 1 and Fig. 2 as can be known, the liquid stream microenvironment simulator that a kind of bacterium BF forms, it is characterized in that: this simulator comprises constant temperature shaking table 1, places the container that holds material 2 in the described constant temperature shaking table 1, and the BF carrier 3 that is fixed in described container 2 bottoms.

Above-mentioned BF carrier 3 is the biofilm load carrier of simulated blood vessel wall or urinary catheter wall; In order to study conveniently, once use this simulator to obtain many group experimental datas, described BF carrier series connection can be fixed on the slide glass 4, be fixed in described container 2 bottoms again.

Said vesse 2 is an open containers, and this example is an Erlenmeyer flask; When using this simulator, the material that holds in the described container 2 is a bacterium liquid 5; Be fixed in placed in-line BF carrier 3 on the slide glass 4 and place bacterium liquid 5 in the container 2, and be fixed in container 2 bottoms.

During use, this simulator is carried out sterilising treatment, under aseptic condition, in container 2, pour cultured bacterium liquid 5 into, seal container 2.Be placed in the constant temperature shaking table 1 that configures again and cultivate, measure the bacterium flow velocity: flow velocity S=L/T, L are girth, and T is the time.Perimeter L=2 π r (π: pi, r: be the distance of the BF carrier to the slide glass of the center of circle at the bottom of the Erlenmeyer flask bottle); Perhaps measure by the hydrometic propeller of measuring flow rate of liquid.During 120 rev/mins of shaking speed, the flow velocity of bacterium liquid is 50.2cm/s.Health adult's arteria cerebri media diastasis blood flow rate (42.79～13.72) cm/S of this and bibliographical information is suitable.

Certainly, this container 2 round bottom microorganism culturing container that can also be beaker or other types; BF carrier 2 can also be other material layers that are used to adhere to culturing micro-organisms; Such conversion all drops on protection domain of the present utility model and so on.

Claims

1. the liquid of a bacterium BF formation flows the microenvironment simulator, and it is characterized in that: this simulator comprises constant temperature shaking table (1), places the container that holds material (2) in the described constant temperature shaking table (1), and the BF carrier (3) that is fixed in described container (2) bottom.

2. the liquid stream microenvironment simulator that bacterium BF as claimed in claim 1 forms, it is characterized in that: described BF carrier (3) is the BF carrier of simulated blood vessel wall or urinary catheter wall.

3. the liquid stream microenvironment simulator that bacterium BF as claimed in claim 1 or 2 forms, it is characterized in that: described BF carrier (3) series connection is fixed on the slide glass (4), is fixed in described container (2) bottom again.

4. the liquid stream microenvironment simulator that bacterium BF as claimed in claim 1 or 2 forms, it is characterized in that: described container (2) is an open containers; The material that holds in the described container (2) is bacterium liquid (5); The BF carrier (3) that is fixed on the slide glass (4) places the interior bacterium liquid (5) of described container (2), and is fixed in described container (2) bottom.

5. the liquid stream microenvironment simulator that bacterium BF as claimed in claim 3 forms, it is characterized in that: described container (2) is an Erlenmeyer flask; The material that holds in the described Erlenmeyer flask is bacterium liquid (5); The placed in-line BF carrier (3) that is fixed on the slide glass (4) places the interior bacterium liquid (5) of Erlenmeyer flask, and is fixed in the Erlenmeyer flask bottom.