CN201314905Y - Special chromatographic column - Google Patents
Special chromatographic column Download PDFInfo
- Publication number
- CN201314905Y CN201314905Y CNU200820227153XU CN200820227153U CN201314905Y CN 201314905 Y CN201314905 Y CN 201314905Y CN U200820227153X U CNU200820227153X U CN U200820227153XU CN 200820227153 U CN200820227153 U CN 200820227153U CN 201314905 Y CN201314905 Y CN 201314905Y
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- CN
- China
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- chromatographic column
- filter core
- mount pad
- post housing
- core mount
- Prior art date
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- Expired - Fee Related
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Abstract
A special chromatographic column includes a tubular cylindrical casing and a connecting piece sleeved thereon. The chromatographic column is characterized in that a built-in filter element mounting base is arranged between the connecting piece and the cylindrical casing; the filter element mounting base is a cylinder with a through hole in axial alignment with the cylindrical casing; a ring-shaped bulge matched with the inner periphery of the cylindrical casing is formed on the joint surface of the filter element mounting base and the cylindrical casing; a groove for a filter element to be embedded in is formed on the inner side of the ring-shaped bulge; and a sealing washer is arranged on the outer side of the ring-shaped bulge.
Description
Technical field
The utility model relates to the pharmaceutical test apparatus, relates in particular to a kind of high molecular polymer and detects chromatographic column specially.
Background technology
Beta-Lactam antibiotic is the broad-spectrum antibiotic of widely used determined curative effect clinically, effect safety, and toxic and side effect is little, but also fatefulue type can take place.Modern study shows, the anaphylactogen that causes the beta-Lactam antibiotic type is not a beta-Lactam antibiotic itself, but the high molecular polymer that wherein exists is the emphasis of current microbiotic macromolecule impurity quality control to the control of endogenous polymkeric substance.The researcher of China is in the anaphylactoid process that studies for a long period of time to beta-Lactam antibiotic, set up the detection method of superpolymer in the gel chromatography Self-control method mensuration beta-Lactam antibiotic in the world first, make the high molecular polymer in the sample realize separating by sephadex G-10 chromatographic column, thereby the content of high molecular polymer in the sample is measured with the common molecule of medicine.Chinese Pharmacopoeia version in 2005 has been formulated the quality control method of tens beta-Lactam antibiotics such as CTX, cefoperazone.
Though this method is in theory clear substantially, present corresponding supporting instrument and equipment and imperfection.Mainly be that each laboratory of employed sephadex chromatography post is very inconsistent, the sephadex column that a lot of laboratories use glass tube column to fill now, its caliber is not of uniform size to be caused, the length of glass tube is also inequality, topmost defective is that chromatographic column needs now to fill before each use, uses extremely inconvenience, influences detection time, and the reappearance that detects is difficult to guarantee, and then influences result's accuracy.With chromatographic the connection also be a weakness of this glass column, because it is similar to a kind of semi-open chromatographic column, its leakproofness is relatively poor, and the flow rate of liquid degree of accuracy is low, and fluctuation is big, filler easily pollutes, and interface is also unified mutually with present widely used standard high performance liquid chromatograph, and what often use is special testing instrument, because of its output little, purpose of design is single, and performance index such as flow velocity precision and detection sensitivity etc. all have certain gap with present widely used high performance liquid chromatograph.The chromatographic column specially of record is by joint in China's utility model patent 00214544.8, and web member and post housing are formed, and spinning in twos to compress is arranged at two filter discs between the annular sealing gasket, realizes sealing.The shortcoming of this technical scheme is: 1, parts are many, complex structure; 2, sealing effectiveness is bad, causes leakage to cause the failure of an experiment through regular meeting; 3, the liquid road is long, and post is pressed high.
Summary of the invention
The utility model provides a kind of high molecular polymer to detect chromatographic column specially at the deficiency that prior art exists.
Technical characterictic of the present utility model is: the post housing of tubulose and the web member that is sleeved on the post housing, the filter core mount pad of dress in it is characterized in that between described web member and post housing, being provided with, described filter core mount pad is the right cylinder that has with described post housing axial alignment through hole, the one side that described filter core mount pad is connected with the post housing be provided with the post housing in week suitable annular projection, the groove of setting-in filter core is set in described annular projection inboard, packing washer is arranged in described annular projection arranged outside.
It is horn-like opening that concrete structure of the present utility model also has the through hole on the described filter core mount pad
Described filter core mount pad is a stainless steel filter core mount pad.
Spin between described web member and the post shell.
Be provided with groove in described post housing outer surface, make things convenient for clamping in order to dismounting.
The beneficial effects of the utility model are: owing to removed joint, reduced the link that connects and effectively dwindled the liquid road, post pressure drop to 20 Pascal; Adopt the filter core mount pad to efficiently solve sealing problem, stopped the phenomenon of leakage; What use is standard liquid chromatography interface, makes the post housing directly to be connected on the high performance liquid chromatograph, good airproof performance, and no leakage, analysis time is short; The post housing adopts stainless-steel tube, system's good leak tightness, no leakage, make it use and preserve all and conveniently, make filler avoid environmental pollution, can tolerate certain pressure, can directly be connected with high performance liquid chromatograph, make the favorable reproducibility of measuring polymeric impurities content in the medicine, relative error obviously reduces, and chromatogram system equilibration time and sample analysis time significantly shorten, this chromatographic column convenient filling, economical and practical, can be widely used in the mensuration of pharmaceutical polymer impurity content.
The tool interface system of standard can be so that all at present common liquid chromatographs can both have no this post of connection of difficulty.For the chromatographic column of this filler of sephadex, after using certain hour, inevitably can produce post and imitate the problem that reduces, our this chromatographic column can be regenerated easily, and need not dismantle, and reduces user's experimental cost and maintenance cost greatly.Chromatographic column structure is simple, and is easy to maintenance, thus the little performance that improved of dead volume.Strengthened the precision of durability, operability and the test findings of method.Shortened detection time greatly, improved work efficiency.In the actual detected, this chromatographic column all can well reach requirement of experiment to the detection of the microbiotic high molecular polymer that Chinese Pharmacopoeia version in 2000 and version in 2005 are recorded, and the number of theoretical plate of blue dextran 2000 and tailing factor are apparently higher than the limit value of Chinese Pharmacopoeia.In the sample detection, the superpolymer peak of each kind is also very desirable with separating of common drug molecule peak, and repeatedly the relative standard deviation of retention time of Ce Dinging and peak area (RSD) is apparently higher than the regulation of Chinese Pharmacopoeia.Simultaneously, its leakproofness is greatly improved, and makes that the preservation of chromatographic column is very convenient, can use as the chromatographic column repeated multiple times of common sample, and preservation condition requires also very low, can long-term storage after being full of preservative substance.And also make filler avoid environmental pollution, keep excellent activity.Can be widely used in the pharmaceuticals industry for the high molecular polymer inspection in the beta-Lactam antibiotic.
Description of drawings
Fig. 1 is a structural representation of the present utility model;
Fig. 2 is a filter core mount pad structural representation.
Among the figure: 1, web member port screw thread, 2, web member, 3, the filter core mount pad, 4, sealing gasket, 5, filter core, 6, the cylinder screw thread, 7, groove, 8, filler, 9, through hole, 10, the web member internal thread, 11, the post housing, 12, annular projection.
Embodiment
As shown in Figure 1, the utility model comprises web member 2 and post housing 11, the filter core mount pad 3 of dress between described web member 2 and post housing 11, being provided with, described filter core mount pad 3 is for having the right cylinder with described post housing 11 axial alignment through holes 9, described filter core mount pad 3 be connected with post housing 11 one and be provided with and post housing 11 in week suitable annular projections 12, at described annular projection 12 inboard setting-in filter cores 5, sealing gasket 4 is arranged in described annular projection 12 arranged outside.
Concrete structure of the present utility model also has the through hole 9 on the described filter core mount pad 3 to be horn-like opening diffusion hole.Described filter core mount pad 3 is a stainless steel filter core mount pad.Spin between described web member 2 and the post housing 11.Be provided with groove 7 at described post housing 11 outside surfaces, make things convenient for clamping in order to dismounting.
During use, the joint of liquid chromatograph is screwed in the web member port screw thread 1, make it with filter core mount pad 3 on the hydraucone of through hole 9 compress and get final product.What chromatographic column specially used is standard liquid chromatography interface, can directly connect by the joint on the liquid chromatograph, make moving phase pass through web member and directly arrive the post bed, and good airproof performance, no leakage, shorten analysis time greatly.
Claims (5)
1, a kind of chromatographic column specially, it comprises the post housing and the web member that is sleeved on the post housing of tubulose, the filter core mount pad of dress in it is characterized in that between described web member and post housing, being provided with, described filter core mount pad is the right cylinder that has with described post housing axial alignment through hole, the one side that described filter core mount pad is connected with the post housing be provided with the post housing in week suitable annular projection, the groove of setting-in filter core is set in described annular projection inboard, packing washer is arranged in described annular projection arranged outside.
2, chromatographic column specially according to claim 1 is characterized in that the through hole on the described filter core mount pad is horn-like opening.
3, chromatographic column specially according to claim 1 is characterized in that described filter core mount pad is a stainless steel filter core mount pad.
4,, it is characterized in that spinning between described web member and the post housing according to claim 1 or 2 or 3 described chromatographic column speciallies.
5,, it is characterized in that being provided with groove in described post housing outer surface according to claim 1 or 2 or 3 described chromatographic column speciallies.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU200820227153XU CN201314905Y (en) | 2008-12-16 | 2008-12-16 | Special chromatographic column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU200820227153XU CN201314905Y (en) | 2008-12-16 | 2008-12-16 | Special chromatographic column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201314905Y true CN201314905Y (en) | 2009-09-23 |
Family
ID=41126704
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU200820227153XU Expired - Fee Related CN201314905Y (en) | 2008-12-16 | 2008-12-16 | Special chromatographic column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201314905Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102841168A (en) * | 2011-06-20 | 2012-12-26 | 苏州赛分科技有限公司 | Liquid chromatographic column |
| CN115569674A (en) * | 2022-09-29 | 2023-01-06 | 中国科学院地球环境研究所 | Ion exchange chromatographic column capable of being rapidly mounted and dismounted |
-
2008
- 2008-12-16 CN CNU200820227153XU patent/CN201314905Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102841168A (en) * | 2011-06-20 | 2012-12-26 | 苏州赛分科技有限公司 | Liquid chromatographic column |
| CN115569674A (en) * | 2022-09-29 | 2023-01-06 | 中国科学院地球环境研究所 | Ion exchange chromatographic column capable of being rapidly mounted and dismounted |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090923 Termination date: 20101216 |