CN201299855Y - Drug feed device used for animal in vivo experiments of drug induced infusion phlebitis - Google Patents
Drug feed device used for animal in vivo experiments of drug induced infusion phlebitis Download PDFInfo
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- CN201299855Y CN201299855Y CNU2008201543128U CN200820154312U CN201299855Y CN 201299855 Y CN201299855 Y CN 201299855Y CN U2008201543128 U CNU2008201543128 U CN U2008201543128U CN 200820154312 U CN200820154312 U CN 200820154312U CN 201299855 Y CN201299855 Y CN 201299855Y
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Abstract
A drug feed device used for animal in vivo experiments of drug induced infusion phlebitis comprises injectors, three-way connecting pipes with valves, a tiny injection constant speed pump and a bracket thereof, an injection speed controller and an infusion needle and is characterized in that the three-way connecting pipe A, the three-way connecting pipe B and the three-way connecting pipe C are sleeved and connected in sequence so as to form two outlets and three inlets; wherein, the outlet of the three-way connecting pipe A is connected with an infusion tube of the infusion needle, the outlet of the three-way connecting pipe C is ensealed; besides, the injector A, the injector B, and the injector C arranged on the bracket are respectively sleeved and connected with the three inlets of the three-way connecting pipe and are respectively connected with the tiny injection constant speed pump connected with the injection speed controller, the duration injection times of three kinds of drugs are respectively controlled by an exterior electronic timer. The device can be used for the establishment of infusion phlebitis models and the research of intervention effect caused by different animals, different drugs and the combination thereof, and has the advantages of good repeatability, high accuracy, simple operation, convenient observation, flexible assembly and wide application.
Description
Technical field
The invention belongs to medical care evaluation, detection technique field, be specifically related to a kind ofly be used to study the relevant influence factor of medication infusion induced phlebitis (drug induced infusion phlebitis) pathogenesis, course of disease characteristics and medication, and the animal of setting up standard dosage regimen and effectively prophylactico-therapeutic measures is in body experiment doser and method thereof.
Background technology
The medication infusion induced phlebitis is one of clinical common drug-induced disease, and its sickness rate height, harm are bigger, cause a lot of unnecessary physiology, mental anguish and medical expenses to patient.Clinical prevention is badly in need of standard scheme and effective measures.
At present, the medication infusion induced phlebitis lacks comparatively ideal animal model, mainly shows: (1) traditional thrombophlebitis animal model can not embody the contribution of the different stimulated medicine of intravenous route administration to the phlebitis result; (2) different pharmaceutical causes that in infusion process phlebitic probability difference is very big, is difficult to represent other drug with a kind of medicine modeling; (3) medicine forms phlebitis and influenced by a lot of process factors such as medicine preparation solvent kind, diluted concentration, injection speed in infusion process, can't be with the influence degree of these variable factors of Research of Animal Model for Study of rigid condition; (4) the normal clinically phlebitis of using another kind or several intravenously administrables (as glucocorticoid, NSAID (non-steroidal anti-inflammatory drug), antihistaminic, vasodilation, local anesthetic, anticoagulant medicine, thrombolytic medicine even medicine etc.) to prevent this medicine to cause, but how its preventive effect can not get on to investigate at the animal model of rigid condition, and if these intervention medicines carry out the secondary puncture injection, this puncture procedure itself just may the The Study of Interference result.For this reason, be necessary to design and a kind ofly can satisfy the demand, be used to study the zoopery doser that medicine causes the transfusion induced phlebitis, remedy the deficiency of common phlebitis model.
Have not yet to see the animal that is exclusively used in the medication infusion induced phlebitis and test doser at body, similarly the chemotherapeutic phlebitis animal model often adopts rabbit ear edge and mouse tail vein injection administration modeling with it, but because the doser that lacks conditional stability and easily adjust flexibly, often can only hand control administration condition, the suitable research range of model itself is dwindled greatly.This class model remains in following problem, as: local venous injury is because of dosage, administration capacity, the different local response differences that show of injection speed, and hand control is difficult to guarantee the stability of these conditions; Administration time prolongs, the also corresponding increase of venous injury, and pain often causes animal acutely to struggle repeatedly and influences operation, and drug leakage very easily takes place; Administration is too fast, can cause animal heart failure to occur and dies suddenly, and makes modeling unsuccessful; Do not guarantee the stability of modeling.
Pass through animal experiment study, our design and assembly this device, success rate, the stability of original animal model had both been improved, increase the motility that model is applicable to various different research purposes again, especially be convenient to carry out different pharmaceutical, different solvents, different dosing speed, the different experiment comparative study of intervening factors such as medicine and different dosing interference method thereof.
Summary of the invention
The purpose of this utility model is to provide a kind of doser and relevant evaluation method thereof that is used for medication infusion induced phlebitis animal in the body experiment.
This utility model is based on the basis of automatic micro-injection pump, is designed repacking, can be used for different animals, different pharmaceutical and combination thereof simultaneously and causes infusing among the researchs such as foundation, influence factor's the investigation of induced phlebitis model and intervention effect evaluation.
The required material of construction device is: the micro-injection pump (multichannel) that U.S. BSA company produces, disposable sterilized injector (using different model) according to the experiment needs, the disposable venous infusion needle head, tee T (the band valve uses varying number according to the experiment needs).Electronic timer (being accurate to second).
The purpose of this utility model is achieved in that a kind of doser that is used for medication infusion induced phlebitis animal in the body experiment, form by disposable syringe, the three-limb tube that has valve, micro-injection constant speed pump, micro-injection constant speed pump support, injection speed controller, disposable infusion syringe needle, it is characterized in that 3 three-limb tube A, B, C sockets successively that have valve, form 2 outlets, 3 inlets; The outlet of three-limb tube A is connected with the tube for transfusion of infusion needle, and the outlet of three-limb tube C is closed; Be placed in 3 disposable syringe A, B on the micro-injection pump support, C respectively with 3 inlets sockets of three-limb tube; The micro-injection constant speed pump that is placed on the micro-injection pump support is connected with 3 disposable syringe A, B, C respectively, and is connected with the injection speed controller, controls the injection speed of 3 syringes respectively; The sub-timer of distribution is controlled the continuous injection time of 3 kinds of medicines respectively in addition.
The construction method (is example with 3 passages) of device is:
At first 3 tee T A, B, C socket are successively got up, can have 5 passages this moment, wherein is up and down 2 outlets, laterally is 3 inlets, and an outlet of C tee T is wherein sealed; Then 3 disposable syringe A, B, C that are filled medicinal liquid are socketed in successively three porch wherein, form 3 passages and be respectively passage A (A medicine), B (B medicine), C (C medicine), and these 3 disposable syringes are placed in respectively on the micro-injection pump support; Control the injection speed of 3 kinds of medicines by the injection speed controller respectively by being placed in 3 micro-injection constant speed pumps on the micro-injection pump support; Tube for transfusion with the disposable infusion syringe needle is connected on the exit passageway of three-limb tube A at last, is used for the animal puncture.The electronic timer of joining outward is used for controlling respectively the continuous injection time of 3 kinds of medicines.
During practical application, utilize electronic timer to control the continuous injection time of every kind of medicine respectively; Common 3 kinds of medicines are to inject successively or alternately.For example, if the program of administration is with the speed Va medicine A of injection Ta time, then with the speed Vb medicine B of injection Tb time, at last with the speed Vc medicine C of injection Tc time.Then concrete setting and operational approach are: the valve of regulating three-limb tube A earlier seals itself and tee T B, starts micro-injection pump A by the injection speed Va that is provided with again, and to close this syringe pump behind the electronic timer control time Ta; Then three-limb tube A is placed the inlet closed state, seal between three-limb tube B and the C, start micro-injection pump B with injection speed Vb thereupon, and to close this syringe pump behind the electronic timer control time Tb; Again three-limb tube B is placed the inlet closed state, start micro-injection pump C with injection speed Vc, and to close this syringe pump behind the electronic timer control time Tc.Above treatment sequence can be because of research demand difference independent assortment, and operational approach is similar.
This device is compared with traditional phlebitis manual operations modeling mode, can simulate probability and overall process that the sequential intravenously administrable of clinical many group transfusions causes phlebitis to take place preferably, overcome the shortcoming of traditional-handwork modeling method repeatability and less stable, has good reproducibility, advantages such as cogency is strong, while can carry out observing at body to the effect that various medicines adopt different dosing regimes to cause phlebitic possibility degree and the different intervention of application medicines to carry out therapeutic intervention, has followed the similarity with human diseases, repeatability, reliability, the suitability, controllability, principles such as easy row property.In addition, the structure of device can be adjusted flexibly according to the experiment needs, the intravenously administrable kind and the administration channel of increase and decrease varying number, and the suitability is more extensive.
Description of drawings
Fig. 1 is used for the zooperal doser sketch map of medication infusion induced phlebitis for this utility model
Fig. 2 schemes because of the transfusion induced phlebitis causes the swelling degree over time for the mouse tail tissue
1 three-limb tube A, 2 three-limb tube B, 3 three-limb tube C, 4 disposable infusion syringe needles, 5 disposable syringes, 6 micro-injection constant speed pump supports, 7 micro-injection constant speed pumps, 8 injection speed controllers, 9 electronic timers, 10 laboratory animals among the figure.
The specific embodiment
Now in conjunction with the embodiments, the using method and the result of use of this device are described in detail.
1. laboratory animal and material
Animal: healthy adult C57 mice, 6-8 week, the cleaning level, body weight is 20.0g-26.0g, and is female, available from Chinese Academy of Sciences's Shanghai Experimental Animal Center.Laboratory animal occupancy permit number: SYXK (Shanghai) 2007-0003.
Device: the medication infusion induced phlebitis animal that this utility model is set up is tested doser at body.
As shown in Figure 1: 3 three-limb tube A, B, C sockets successively that have valve form 2 outlets, 3 inlets; The outlet of three-limb tube A is connected with the tube for transfusion of infusion needle, and the outlet C of three-limb tube C is closed; Be placed in 3 disposable syringe A, B on the micro-injection pump support, C respectively with 3 inlets sockets of three-limb tube; The micro-injection constant speed pump that is placed on the micro-injection pump support is connected with 3 disposable syringe A, B, C respectively mutually, and is connected with the injection speed controller, controls the injection speed of 3 syringes respectively; The sub-timer of distribution is controlled the continuous injection time of 3 kinds of medicines respectively in addition.
Wherein, in the A syringe be 0.9% sodium chloride injection; In the B syringe is cimetidine inj; In the C syringe is the Vinorelbine monotartrate injection.
Medicine: Vinorelbine monotartrate injection (VIN; Trade name: Gai Nuo, Jiangsu Haosen Pharmaceutical Co., Ltd., Lianyungang; Specification: 1mg/ml);
Cimetidine inj (CIM; Sino-America Tianjin Shike Pharmaceutical Co., Ltd.'s packing, German WulfingPharma.GmbH makes; Specification: 0.2g/2ml);
0.9% sodium chloride injection (NS; Hundred special medicine company limiteies; Specification: 250ml);
10% formalin solution: get 10ml formalin, be diluted to 100ml, 4 ℃ of preservations with 0.1M PBS.
2. experiment purpose: investigate under the conditions such as specific administration dosage, injection speed, administration concentration, the Vinorelbine monotartrate injection causes the infuse pathogenic process and the pathological characteristic of induced phlebitis, and studies cimetidine inj prevention this phlebitic effect and dosage regimen.
3. experimental technique: mouse tail vein is injected VIN and is made chemotherapeutic phlebitis modelling and intervention study:
Grouping: get 45 of C57 mices, be divided into 3 groups at random, be respectively NS blank group, VIN stimulation administration group and cimetidine and intervene administration group, 15 every group.
(1) the blank group of NS: tail intravenous injection NS 325 μ l;
(2) VIN stimulates the administration group: tail intravenous injection VIN 75 μ l and NS 250 μ l;
(3) cimetidine is intervened the administration group: tail intravenous injection CIM 250 μ l and VIN 75 μ l; Illustrate: when injection NS and cimetidine, microdialysis pump implementation speed places " 100 " shelves, and inject time and dosage convert; During injection VIN, microdialysis pump implementation speed places " 20 " shelves, and inject time and dosage convert.
When injecting VIN and cimetidine, be NS in the public passage, it can be used as the injection of leading the way at every turn, and determining punctures successfully injects chemotherapeutics again, injects the normal saline washing pipe thereafter, reduces medicine partial residual.
Select for use mouse tail vein to inject medicine, puncture is success once, otherwise changes animal.Each treated animal administration was taken a picture after 12 hours, observe outward appearance phlebitis situation occurred, and fix a position measurement Mus tail swelling degree, put to death animal with the cervical vertebra dislocation method in 7 days after the injection, and get near the Mus tail tissue point of puncture, place 10% formalin solution to fix more than 4 hours rapidly, after carrying out decalcification and handled for 1 week with 20%EDTA, be made into paraffin section, H-E dyeing and further analyze.According to record Mus tail diameter every day, statistics is respectively organized difference; Observe relevant endothelial denudation, inflammatory cell infiltration, thrombosis and edema situation down according to the pathological section mirror and investigate its transfusion induced phlebitis dependency pathological change.
3. experimental result mouse tail tissue is seen Fig. 2 because of the variation of swelling degree due to the phlebitis.
Experiment shows: mouse tail vein is injected VIN and is made the chemotherapeutic phlebitis model, inject medicine after blood vessel shrink immediately, bleach, point of puncture and surrounding tissue have scleroma; And inject the normal saline group and intervention group does not have significant change, indivedual afterbodys are swollen; Inject after 24 hours and to observe the injecting normal saline group and intervention group injection site skin color majority transfers to normally, and it is rubescent to inject VIN group injection site, afterbody swelling, up to common phlebitis symptoms such as the 4th day injection site erythema, edema, streak change, scleromas, more have and seriously fester and bad the dead occurs.Observe the H-E stained of animal model, the normal saline group except that slight tissue edema, the endotheliocyte marshalling, other are normal substantially; VIN processed group vein blood vessel cavity segment endothelial denudation is managed all tissue edemas, a large amount of cell infiltration; Indivedual stimulating group are especially serious, and lumen of vessels enlarges, the visible thrombosis of part intravenous; Intervention group and VIN group are at endothelial denudation, and thrombosis, cell infiltration all have significant difference.As shown in Figure 2: intervention group afterbody swelling degree all has significant difference with blank group swelling degree no difference of science of statistics, VIN group afterbody swelling degree with blank group and intervention group.
The above results shows that the medication infusion induced phlebitis animal that this utility model is set up in the foundation of phlebitis model is apparent in the body experiment effect that doser played.Can simulate phlebitis dynamic response overall process preferably, and be not limited to mouse model, for the damage mechanism and the protectiving scheme of further investigation transfusion induced phlebitis have been made useful exploration.
Claims (1)
1. one kind is used for the doser that medication infusion induced phlebitis animal is tested at body, form by disposable syringe, the three-limb tube that has valve, micro-injection constant speed pump, micro-injection constant speed pump support, injection speed controller, disposable infusion syringe needle, it is characterized in that 3 three-limb tube A, B, C sockets successively that have valve, form 2 outlets, 3 inlets; The outlet of three-limb tube A is connected with the tube for transfusion of infusion needle, and the outlet of three-limb tube C is closed; Be placed in 3 disposable syringe A, B on the micro-injection pump support, C respectively with 3 inlets sockets of three-limb tube; The micro-injection constant speed pump that is placed on the micro-injection pump support is connected with 3 disposable syringe A, B, C respectively, and is connected with the injection speed controller.
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| CNU2008201543128U CN201299855Y (en) | 2008-10-22 | 2008-10-22 | Drug feed device used for animal in vivo experiments of drug induced infusion phlebitis |
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| CNU2008201543128U CN201299855Y (en) | 2008-10-22 | 2008-10-22 | Drug feed device used for animal in vivo experiments of drug induced infusion phlebitis |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104274254A (en) * | 2013-07-08 | 2015-01-14 | 王薇 | Accurate quantification device for animal vein puncture dosing |
| CN104323868A (en) * | 2014-11-06 | 2015-02-04 | 中国人民解放军第四军医大学 | Micro-infusion method and device thereof based on epididymis duct intracavity environment experiment |
| CN104971400A (en) * | 2015-07-15 | 2015-10-14 | 广西南宁灵康赛诺科生物科技有限公司 | Method for long-term intracerebral pump injection of drugs in great quantity used for primate |
| CN106109053A (en) * | 2016-08-20 | 2016-11-16 | 王炎强 | A kind of experiment caudal vein microsyringe |
-
2008
- 2008-10-22 CN CNU2008201543128U patent/CN201299855Y/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104274254A (en) * | 2013-07-08 | 2015-01-14 | 王薇 | Accurate quantification device for animal vein puncture dosing |
| CN104274254B (en) * | 2013-07-08 | 2016-06-01 | 中国医学科学院北京协和医院 | Animal venipuncture administration accurate quantification device |
| CN104323868A (en) * | 2014-11-06 | 2015-02-04 | 中国人民解放军第四军医大学 | Micro-infusion method and device thereof based on epididymis duct intracavity environment experiment |
| CN104971400A (en) * | 2015-07-15 | 2015-10-14 | 广西南宁灵康赛诺科生物科技有限公司 | Method for long-term intracerebral pump injection of drugs in great quantity used for primate |
| CN106109053A (en) * | 2016-08-20 | 2016-11-16 | 王炎强 | A kind of experiment caudal vein microsyringe |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090902 Termination date: 20171022 |
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| CF01 | Termination of patent right due to non-payment of annual fee |