CN201239138Y - Integral fluorescence transmission image-forming device for small animal - Google Patents
Integral fluorescence transmission image-forming device for small animal Download PDFInfo
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- CN201239138Y CN201239138Y CNU2008200671398U CN200820067139U CN201239138Y CN 201239138 Y CN201239138 Y CN 201239138Y CN U2008200671398 U CNU2008200671398 U CN U2008200671398U CN 200820067139 U CN200820067139 U CN 200820067139U CN 201239138 Y CN201239138 Y CN 201239138Y
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- flat panel
- panel detector
- plane mirror
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Abstract
The utility model discloses an imaging device with integral fluorescence transmission for beasties, which has the structure that a flat panel detector, an object stage, a flat mirror and an X-ray source are arranged in sequence from bottom to top, and the centers of the components are positioned on the same straight line; two luminescent diodes positioned on the same horizontal line are respectively positioned above both sides of the ray source; and an optical filter and an electriccharge coupler are positioned on one side of the non-working surface of the flat mirror and share the same shaft, and the shaft lines thereof pass through the center of the flat mirror; and the flat panel detector and the electriccharge coupler are connected with a computer by a data collecting card. The device utilizes the advantage of structure imaging of the digital X-ray transmission and imaging technology to solve the problem that the prior integral fluorescent optics imaging technology inaccurately positions tumors. The device fuses images gained by the digital X-ray transmission and imaging technology and the integral fluorescent optics imaging technology through the computer so as to gain more detailed tumor information, thus the device provides the research of tumor invasion, growth and metastasis with an effective tool.
Description
Technical field
This utility model belongs to the biomedical imaging technology, be specifically related to a kind of integral fluorescence transmission imaging device that is used for toy, this device can carry out digital X-ray transmission imaging and integral fluorescence optical imagery to toy simultaneously, and it is applicable in the biomedicine research to tumor.
Background technology
Digital X-ray transmission imaging technology be at present comparatively mature technique and be successfully applied to clinical.This technology has very important effect for the research of tumor disease, can provide toy detailed structural information.The integral fluorescence optical image technology has been brought into play very big effect in detection tumor, research fields of tumor.In Chinese patent literature CN1773258 (open day is on May 17th, 2006), use integral fluorescence optical image technology device that toy is carried out tumor research, but its imaging is second-rate, the location positioning of tumor is inaccurate, and this invention is not mentioned and utilized digital X-ray transmission imaging technology not compensate integral fluorescence optical image technology imaging effect is good.
Summary of the invention
The purpose of this utility model is to provide a kind of integral fluorescence transmission imaging device that is used for toy, and this device can solve and can have the integral fluorescence optical image technology technology problem inaccurate to tumor-localizing now.
The integral fluorescence transmission imaging device that is used for toy that this utility model provides is characterized in that: this device comprises x-ray source, flat panel detector, data collecting card, object stage, plane mirror, electric charge Rhizoma Nelumbinis and device, optical filter, first, second light emitting diode and computer;
Object stage is positioned at flat panel detector top, and plane mirror is positioned at the object stage top, and with horizontal line in angle of 45 degrees; X-ray source is positioned at the top of plane mirror, being centered close on the same straight line of flat panel detector, object stage, plane mirror and x-ray source; First, second light emitting diode is positioned at top, radiogenic both sides, and the center of first, second light emitting diode is on the same horizontal line;
Optical filter and electric charge Rhizoma Nelumbinis and device are positioned at non-working surface one side of plane mirror, and optical filter and electric charge Rhizoma Nelumbinis and device are coaxial, and its axis is by the center of plane mirror;
Flat panel detector is connected with computer by data collecting card with charge-coupled device.
This utility model utilizes the advantage of digital X-ray transmission imaging technology aspect structure imaging, has solved the existing integral fluorescence optical image technology problem inaccurate to tumor-localizing.Whole device midplane mirror is most important, thereby is surveyed by electric charge Rhizoma Nelumbinis and device through flat mirror reflects by large-power light-emitting diodes institute excited fluorescent.Simultaneously can carry out the imaging of digital X-ray transmission imaging technology, owing to X ray does not exert an influence to common electrical Rhizoma Nelumbinis and device imaging, so can realize digital X-ray transmission imaging technology and the imaging simultaneously of integral fluorescence optical image technology to sample.This utility model provides effective research tool for invasion, growth and the transfer of research tumor.
Description of drawings
Fig. 1 is the structural representation of this utility model integral fluorescence transmission imaging device;
Fig. 2 excites light wavelength and launching efficiency relation curve for green fluorescent protein;
Fig. 3 is the light emitting diode and the 520 high-pass filter curves of spectrum;
Fig. 4 is the response curve of electric charge Rhizoma Nelumbinis and device.
The specific embodiment
Below in conjunction with accompanying drawing and example this utility model is described in further detail.
As shown in Figure 1, this utility model integral fluorescence transmission imaging device comprises x-ray source 1, flat panel detector 6, data collecting card 9, object stage 5, plane mirror 7, electric charge Rhizoma Nelumbinis and device 4, optical filter 8, the first light emitting diodes 2, the second light emitting diodes 3, computer 10.
Flat panel detector 6 is in the bottom of device, is the object stage 5 of placing sample above flat panel detector, and the top of object stage 5 is plane mirrors 7 that become miter angle α with horizontal line, and the plane mirror top is an x-ray source 1; The device centrage is crossed radiographic source 1, and the center of plane mirror also is the perpendicular bisector of flat panel detector 6 and object stage simultaneously; First light emitting diode, 2, the second light emitting diodes 3 have been placed in not far position above radiogenic both sides, and these two light emitting diode centers are on the same horizontal line; Placed optical filter 8 and electric charge Rhizoma Nelumbinis and device 4 on the right of plane mirror, and optical filter 8 and electric charge Rhizoma Nelumbinis and device 4 are coaxial and axis is crossed the center of plane mirror.Data collecting card 9 is by digital interface and flat panel detector 6, and charge-coupled device 4 links to each other, and is connected with computer by the PCI slot.
The X ray that is sent by radiographic source 1 accumulates in the included zone of arrow, and the sample that transmission is crossed above the sample stage 5 is surveyed by flat panel detector 6, is conveyed into computer by digital transmission line by data collecting card again.The illumination that light emitting diode 2,3 is sent on 6 and reflection, total reflective mirror 7 emissions of reflected light by becoming miter angle with horizontal plane, computer is surveyed and sent in mating plate 8 back of filtering by electric charge Rhizoma Nelumbinis and device 4 after filtration.
Total reflective mirror and horizontal line angulation are that 45 degree are innovative points of the present utility model, thereby we make can allow in this device and are surveyed by electric charge Rhizoma Nelumbinis and device through flat mirror reflects by large-power light-emitting diodes institute excited fluorescent in this way.The X ray that the while x-ray source is launched obtains the digital X-ray transmission imaging technical pattern information of mice because its extremely strong penetration capacity can be punched plane mirror and be shone object by flat panel detector.
The detector that is used for detecting x-ray in the device is selected flat panel detector for use.Finish digital collection by data collecting card, output to imaging in the computer installation then.
Whole device has two place's data collecting cards, can be asynchronous or the image of two different photographic head of synchronous acquisition.
The electric light source that fluorescence excitation need excite has the luminous power of suitable spectrum and suitable intensity.As Fig. 4 is the excitation spectrum and the fluorescence Spectra of green fluorescent protein.
Road as can be seen from Figure 2, the peak value of the launching efficiency 12 of green fluorescent protein (with its peak value normalization) is positioned at 488 nanometers, in 460～490 nano wavebands greater than 66%.The peak value of the fluorescence of green fluorescent protein (with its peak value normalization) is positioned at 510 nanometers, is about 60% of peak value place in 520 nano fluorescent intensity.Take all factors into consideration fluorescence launching efficiency, collect intensity of fluorescence and background autofluorescence: select the long logical fluorescent optical filter of the exciter filter of 460～492 nanometers and 520 nanometers when being used for the green fluorescent protein imaging for use.
Fluorescent marker is positioned at organism in the integral fluorescence optical image technology, and exciting light need pass through the biological tissue of suitable thickness.Because it is very big that exciting light passes through when organizing decay, the light source that has than high light output just can be used for the integral fluorescence optical image technology.The excitation source that is used for the integral fluorescence optical image technology at present has mercury lamp, laser instrument.But mercury lamp is high-power, broad spectrum light source, and its complicated operation costs an arm and a leg, and the life-span of bulb is generally shorter, and cost of use is higher; Laser instrument is a kind of line spectrum source, and its price is also high, and only be thousands of hours service life.Along with novel suitable device occurs as the light emitting diode more than 3 watts, making up integral fluorescence optical image technology device more cheap, easy to use, that volume is less, light source life is long becomes possibility.
When substituting as new light sources with light emitting diode, the fluorescence signal intensity that imaging device obtains before and after needing to keep substituting is suitable.When other conditions were identical, fluorescence intensity was relevant with the light intensity of light source, the spectral distribution density of light source, the excitation spectrum (also being called absorption spectrum) of fluorescent dye etc.With mercury lamp (50 watts of power) is reference, at λ
1～λ
2Wave band needs the luminous flux of light emitting diode output to be foundation configuration light emitting diode when fluorescence intensity is identical when suitable with mercury lamp excited fluorescent amount.
At λ
1～λ
2Wave band, the formula of fluorescence volume of light source activation that calculates luminous flux and be L is as follows:
In the formula
Be the launching efficiency of fluorescent material, the expression wavelength is λ
iThe efficient (normalization) of light radiation fluorescence excitation material.
Sample calculation: at 420-490 nano wavebands, the ExQu of mercury lamp:
Can be regarded as to such an extent that luminous flux is that the light emitting diode of 100 lumens is at the ExQu of this wave band equally: 68.1, needing the luminous flux of light emitting diode when then ExQu equates is 203.2 (lumens), at 420-490 nano wavebands, with luminous flux be that the mercury lamp of 2000 lumens has suitable fluorescence excitation effect, as long as the luminous flux of light emitting diode matrix is no less than 203.2 lumens.Result of calculation is as shown in table 1 below.
Table 1 is reference with the mercury lamp, needs the luminous flux of light emitting diode when the different-waveband fluorescence excitation
| Band(nm) | 420-490 | 430-490 | 440-490 | 450-490 | 460-490 |
| Light emitting diode (lumen) | 203.2 | 193.9 | 120.7 | 73.8 | 60.4 |
Select 43 watts large-power light-emitting diodes for use according to calculating us.Light emitting diode is blue 3 watts, and wave-length coverage is 460～480 nanometers, and centre wavelength is 470 nanometers.What optical filter was selected for use is 520 high passes.
As from the foregoing, this utility model merges the image that digital X-ray transmission imaging technology and integral fluorescence optical image technology are obtained by computer, thereby obtain more detailed tumor information, for invasion, growth and the transfer of studying tumor provides effective research tool.
The device experiment
The experiment biomaterial: Mus age was 4～5 weeks, body weight 15～20 grams.Animal feeding and experiment require to carry out in strict accordance with People's Republic of China's " management of laboratory animal regulations ".Mice is respectively carried out the green fluorescence solution of 0.1 milliliter of subcutaneous injection, set up mice green fluorescent protein fluorescent sample.Experimental situation: x-ray source voltage is 60 kilovolts, and electric current is 650 milliamperes; Electric charge Rhizoma Nelumbinis and device running voltage are 5 volts of direct currents, and image taking speed is 24 frame per seconds, and Fig. 3 is the response curve of electric charge Rhizoma Nelumbinis and device, from curve as can be seen when wavelength is 520 nanometers its detection efficient reach 55%, very little with highest detectable rate difference; Flat panel detector imaging size 1024*1024, time of exposure is 1 second; As shown in Figure 4, curve 13 optical filters wavelength during greater than 520 nanometers transmitance reach 60%.
Claims (1)
1, a kind of integral fluorescence transmission imaging device that is used for toy is characterized in that: this device comprises x-ray source (1), flat panel detector (6), data collecting card (9), object stage (5), plane mirror (7), electric charge Rhizoma Nelumbinis and device (4), optical filter (8), first, second light emitting diode (2,3) and computer (10);
Object stage (5) is positioned at flat panel detector (6) top, and plane mirror (7) is positioned at object stage (5) top, and with horizontal line in angle of 45 degrees; X-ray source (1) is positioned at the top of plane mirror (7), being centered close on the same straight line of flat panel detector (6), object stage (5), plane mirror (7) and x-ray source (1); First, second light emitting diode (2,3) is positioned at the top, both sides of radiographic source (1), and the center of first, second light emitting diode (2,3) is on the same horizontal line;
Optical filter (8) and electric charge Rhizoma Nelumbinis and device (4) are positioned at non-working surface one side of plane mirror (7), and optical filter (8) and electric charge Rhizoma Nelumbinis and device (4) are coaxial, and its axis is by the center of plane mirror (7);
Flat panel detector (6) is connected with computer (10) by data collecting card (9) with charge-coupled device (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200671398U CN201239138Y (en) | 2008-05-09 | 2008-05-09 | Integral fluorescence transmission image-forming device for small animal |
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| Application Number | Priority Date | Filing Date | Title |
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| CNU2008200671398U CN201239138Y (en) | 2008-05-09 | 2008-05-09 | Integral fluorescence transmission image-forming device for small animal |
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| CN201239138Y true CN201239138Y (en) | 2009-05-20 |
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| CNU2008200671398U Expired - Fee Related CN201239138Y (en) | 2008-05-09 | 2008-05-09 | Integral fluorescence transmission image-forming device for small animal |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102445420A (en) * | 2011-10-13 | 2012-05-09 | 中国科学院上海微系统与信息技术研究所 | Transmission imaging device and method based on tera-hertz quantum device |
-
2008
- 2008-05-09 CN CNU2008200671398U patent/CN201239138Y/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102445420A (en) * | 2011-10-13 | 2012-05-09 | 中国科学院上海微系统与信息技术研究所 | Transmission imaging device and method based on tera-hertz quantum device |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090520 Termination date: 20110509 |