CN112505082A - X-ray near-infrared two-region luminous biological imaging device - Google Patents
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Abstract
The invention discloses an X-ray near-infrared two-region luminous biological imaging device, which comprises: the device comprises a light source module and a light-emitting imaging module; the light emitting imaging module includes: the device comprises an objective lens, a first dichroic mirror, a CCD camera and an InGaAs camera; the light source module is used for providing exciting light for a sample to be imaged, the objective lens is used for collecting fluorescence emitted by the sample to be imaged after the sample to be imaged is excited, and the first dichroic mirror is used for reflecting the fluorescence collected by the objective lens to a light inlet of the CCD camera or the InGaAs camera. In the scheme, the X-ray excitation light path and the imaging camera are cooperatively switched, so that X-ray visible-near infrared two-region luminous imaging is realized, instrument support can be further provided for scintillator material and X-ray luminous analysis research, in-vitro diagnosis and deep living body precise imaging analysis of a complex sample are facilitated, and research and development of scintillator nano materials and leading-edge scientific research and practical application of the scintillator nano materials in the fields of X-ray luminous immunoassay, X-ray luminous imaging, X-ray treatment, X-ray optogenetics research and the like are promoted.
Description
Technical Field
The invention relates to the technical field of X-ray luminescence imaging, in particular to an X-ray near-infrared two-region luminescence biological imaging device.
Background
X-ray excited luminescence of a scintillator material (X-ray luminescence) is a phenomenon in which the scintillator material absorbs high-energy X-rays and converts them into low-energy photons (e.g., visible light, near-infrared light, etc.).
In recent years, X-ray luminescence analysis based on scintillator nanoparticles has been developed as a new method and technology for optical analysis in biomedical research. The X-ray is used as a high-energy photon, has strong penetrating power to living tissues, can effectively eliminate the interference of background fluorescence in the luminous imaging of blood samples, living tissues and the like by the X-ray luminous imaging technology, and has higher imaging signal-to-noise ratio. The technologies such as X-ray immunoassay, high-sensitivity luminescence imaging, radiosensitization treatment, X-ray photodynamic treatment and the like based on the X-ray luminescent scintillator nano particles show a plurality of application advantages in early diagnosis and high-efficiency treatment research of diseases such as tumors and the like. At present, the international colleagues generally believe that the X-ray luminescence biological analysis technology is expected to solve the key scientific problems of high autofluorescence background, low penetration depth, low signal-to-noise ratio and the like of the traditional fluorescence biological analysis technology in blood/living tissues, opens up a new way for biomedical research such as complex sample in-vitro diagnosis and deep living imaging and the like, and has huge clinical transformation potential.
The X-ray luminescence technology shows great application value in the fields of nuclear medicine imaging, radiation monitoring, clinical diagnosis, disease treatment and the like, and recent researches show that the near-infrared two-region (1000nm-1700nm) luminescence and imaging can effectively reduce the tissue background fluorescence existing in visible-near-infrared one-region (400-800nm) luminescence imaging and improve the in-vivo imaging depth. The CCD camera configured by the current commercial X-ray small animal imager has the problems of low sensitivity, single imaging function, small response band (400 + 800nm) and the like, and can only be used for visible-near infrared one-region luminescence imaging, and the blank current situation of the commercial X-ray near infrared two-region luminescence imager at home and abroad greatly limits the application innovation research of in-vitro accurate diagnosis, living body high-sensitivity high-resolution imaging and the like of the scintillator nanometer material.
Disclosure of Invention
In view of the above, the present invention provides an X-ray near-infrared two-region luminescence bio-imaging apparatus, which can realize X-ray visible-near-infrared two-region luminescence imaging, further provide instrument support for scintillator materials and X-ray luminescence analysis research, and facilitate realization of in vitro diagnosis of complex samples and precise imaging analysis of deep living bodies, thereby promoting research and development of scintillator nano materials and leading-edge scientific research and practical application thereof in the fields of X-ray luminescence immunoassay, X-ray luminescence imaging, X-ray therapy, X-ray optogenetic research, and the like.
In order to achieve the purpose, the invention provides the following technical scheme:
an X-ray near-infrared two-zone luminescence bio-imaging device, comprising: the device comprises a light source module and a light-emitting imaging module;
the light source module includes: an X-ray tube for providing an X-ray source for a sample to be imaged;
preferably, the light source module further includes: the extended light source module is used for providing extended exciting light for the sample to be imaged;
preferably, the extended light source module includes: the expanded light source, the reflector, the beam expander and the second dichroic mirror;
the reflector is used for reflecting the light source emitted by the expanded light source to the light inlet of the beam expander, and the second dichroic mirror is used for reflecting the light source coming out of the light outlet of the beam expander to the surface of the sample to be imaged and passing through the fluorescence emitted by the sample to be imaged after being excited by the light source module.
The light emitting imaging module includes: the device comprises an objective lens, a first dichroic mirror, a CCD camera and an InGaAs camera;
the light source module is used for providing exciting light for a sample to be imaged, the objective lens is used for collecting fluorescence emitted by the sample to be imaged after being excited, and the first dichroic mirror is used for reflecting the fluorescence collected by the objective lens to a light inlet of the CCD camera or the InGaAs camera and emitting the fluorescence after the sample to be imaged is excited by the light source module.
Preferably, the method further comprises the following steps: and the filter set is used for filtering fluorescence emitted by the sample to be imaged after being excited.
Preferably, the filter set includes: and the filter set is arranged between the light source module and the objective lens.
Preferably, the method further comprises the following steps: and the X-ray detector is used for detecting fluorescence emitted by the sample to be imaged after being excited by X-rays.
According to the technical scheme, the X-ray near-infrared two-region luminous biological imaging device provided by the invention can conveniently realize X-ray visible-near-infrared two-region luminous imaging through the cooperative switching of the X-ray excitation light path and the imaging camera, further provide instrument support for scintillator materials and X-ray luminous analysis research, and contribute to realizing complex sample in-vitro diagnosis and deep living body accurate imaging analysis, so that the research and development of scintillator nano materials and the leading-edge scientific research and practical application of the scintillator nano materials in the fields of X-ray luminous immunoassay, X-ray luminous imaging, X-ray treatment, X-ray optogenetics research and the like are promoted.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an X-ray near-infrared two-region luminescence bio-imaging apparatus according to an embodiment of the present invention;
FIG. 2 is a schematic diagram illustrating a design concept of an X-ray near-infrared two-region luminescence bio-imaging apparatus according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of a technical route of an X-ray near-infrared two-region luminescence bio-imaging apparatus according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of detection of an X-ray source according to an embodiment of the present invention;
FIG. 5 is a schematic diagram of detection of an extended light source according to an embodiment of the present invention;
FIG. 6 is a time-sharing collection light path diagram for visible-near infrared luminescence imaging according to an embodiment of the present invention;
FIG. 7 is a schematic diagram of a design of an X-ray luminescence imaging module according to an embodiment of the present invention;
FIG. 8 is a technical roadmap for an X-ray luminescence imaging module provided by an embodiment of the present invention;
fig. 9 is a schematic structural diagram of an X-ray near-infrared two-region luminescence bio-imaging apparatus according to another embodiment of the present invention.
The X-ray tube 11 is an X-ray tube, the extended light source 12-1 is a reflector 12-2, the beam expander 12-3 is a beam expander, and the second dichroic mirror 12-4 is a second dichroic mirror; 21 is an objective lens, 22 is a first dichroic mirror, 23 is a CCD camera, and 24 is an InGaAs camera; 30 is a filter set; 50 is an X-ray detector; 60 is a sample table; 70 is the sample to be imaged.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the invention provides an X-ray near-infrared two-region luminous biological imaging device, which comprises: the device comprises a light source module and a light-emitting imaging module;
the light source module includes: an X-ray tube 11 for providing an X-ray source for a sample 70 to be imaged;
preferably, the light source module further includes: an extended light source module for providing extended excitation light for the sample 70 to be imaged;
preferably, as shown in fig. 1, the extended light source module includes: the device comprises an extended light source 12-1, a reflector 12-2, a beam expander 12-3 and a second dichroic mirror 12-4;
the reflector 12-2 is configured to reflect a light source emitted by the extended light source 12-2 to a light inlet of the beam expander 12-3, and the second dichroic mirror 12-4 is configured to reflect a light source coming out of a light outlet of the beam expander 12-3 to a surface of the sample 70 to be imaged, and emit fluorescence after being excited by the light source module through the sample 70 to be imaged.
As shown in fig. 1, the luminescence imaging module includes: an objective lens 21, a first dichroic mirror 22, a CCD camera 23, and an InGaAs camera 24;
the light source module is used for providing exciting light for the sample 70 to be imaged, the objective lens 21 is used for collecting fluorescence emitted by the sample 70 to be imaged after being excited, and the first dichroic mirror 22 is used for reflecting the fluorescence collected by the objective lens 21 to a light inlet of the CCD camera 23 or the InGaAs camera 24.
In the present scheme, it should be noted that the visible-near infrared light first-region imaging CCD camera 23 and the near infrared light second-region luminescence imaging InGaAs camera 24 are integrated by a microcomputer system, that is, the microcomputer system controls the switching between the CCD camera 23 and the InGaAs camera 24, so as to realize the X-ray visible-near infrared light second-region luminescence imaging (400-.
It can be seen from the above technical solutions that, in the X-ray near-infrared two-region luminescence bio-imaging apparatus provided in the embodiment of the present invention, through the cooperative switching of the X-ray excitation light path and the imaging camera, the X-ray visible-near-infrared two-region luminescence imaging is facilitated to be realized, and further, an instrument support is provided for scintillator materials and X-ray luminescence analysis research, which is helpful for realizing the in vitro diagnosis of complex samples and the precise imaging analysis of deep living bodies, thereby promoting the research and development of scintillator nano materials and the leading-edge scientific research and practical application thereof in the fields of X-ray luminescence immunoassay, X-ray luminescence imaging, X-ray therapy, X-ray optogenetic research, and the like.
In the scheme, the research aims of scintillator material development, X-ray luminescence analysis and the like are better met; accordingly, the light source module includes: an X-ray tube 11 in which a technical route of an X-ray luminescence imaging module is shown in fig. 8; in addition, the light source module further includes: and the light source module is expanded so as to realize multifunctional steady-state luminescence test and in-vivo imaging analysis.
In the scheme, in order to reduce the background interference of fluorescence and improve the sensitivity of the camera, the high-sensitivity X-ray visible-near infrared two-region luminous imaging effect is achieved; correspondingly, the X-ray near-infrared two-region luminescence biological imaging device provided by the embodiment of the invention further comprises: and a filter set 30 for filtering fluorescence emitted by the sample 70 to be imaged after excitation.
Further, as shown in fig. 1, filter set 30 includes: and a filter set 30 disposed between the light source module and the objective lens 21. Wherein, filter set 30 is integrated with polylith narrowband filter to in realizing the luminous formation of image of high sensitive multichannel, promote the high-resolution imaging analysis of dynamic high-resolution imaging ability and slight tissue, realize accurate quantitative imaging analysis. In the scheme, the optical filters are selected through switching of the optical filter group 30 to obtain multi-channel different X-ray luminescence imaging images, so that high-sensitivity, high-temporal-spatial-resolution, broadband response (400-1700nm) and quantitative dynamic X-ray luminescence imaging are realized.
Still further, in order to achieve efficient detection of visible-near infrared photons; accordingly, as shown in fig. 1, the X-ray near-infrared two-region luminescence bio-imaging apparatus provided by the embodiment of the present invention further includes: an X-ray detector 50 for detecting fluorescence emitted by the sample 70 to be imaged after excitation. In the present solution, the X-ray detector 50 can be used to realize X-ray photography, and then the organic integration of X-ray photography and X-ray luminescence imaging is used, thereby contributing to the improvement of scientificity, high efficiency and advancement of developing devices. Wherein, the multi-dimensional movement of the X-ray detector 50 is controlled by the microcomputer system so as to realize the omnibearing detection of the X-ray detector 50.
The present solution is further described below with reference to specific embodiments:
in a first embodiment provided by the present invention, the present apparatus can perform X-ray luminescence imaging of scintillator materials. As shown in fig. 4, 7 and 9, a sample 70 to be imaged is placed on the sample stage 60, and the microcomputer system controls the X-ray detector 50 to move transversely and longitudinally, so that the X-ray detector 50 is located at a proper position above the sample 70 to be imaged; the X-ray tube 11 is turned on, X-rays emitted by the X-ray tube 11 penetrate through the sample stage 60 to irradiate the sample 70 to be imaged, and fluorescence emitted by the sample 70 to be imaged after being excited by the X-rays is collected and processed by the X-ray detector 50, and then data is transmitted to the microcomputer system for image output.
In a second embodiment provided by the present invention, the present device can perform visible-near infrared one-region photoluminescence imaging of a scintillator material. As shown in fig. 5, 7 and 9, the sample 70 to be imaged is placed on the sample stage 60, and the microcomputer system controls the X-ray detector 50 to move transversely and longitudinally, so that the X-ray detector 50 is not located at any position between the sample 70 to be imaged and the luminescence imaging module; the microcomputer system controls the filter set 30 to switch and select the filter with the required bandwidth; the microcomputer system controls the first dichroic mirror 22 to rotate to a position which forms an angle of 45 degrees with the vertical direction and faces the CCD camera 23; as shown in fig. 6, the extended light source 12-1 is turned on, the excitation light emitted by the extended light source 12-1 is reflected by the reflector 12-2 and enters the beam expander 12-3, the light beam expanded by the beam expander 12-3 is incident on the second dichroic mirror 12-4 and then reflected to irradiate the sample to be imaged 70, the fluorescence emitted by the sample to be imaged 70 after being excited by the extended light source 12-1 passes through the second dichroic mirror 12-4 and then is filtered by the optical filter to remove background interference light, the fluorescence is collected by the objective lens 21 and is incident on the first dichroic mirror 22, the fluorescence is reflected by the first dichroic mirror 22 and is incident on the CCD camera 23 for collection and processing, and then the data is transmitted to the microcomputer system for image output.
In a third embodiment provided by the present invention, the present device can perform near-infrared two-zone photoluminescence imaging of a scintillator material. As shown in fig. 5, 7 and 9, the sample 70 to be imaged is placed on the sample stage 60, and the microcomputer system controls the X-ray detector 50 to move transversely and longitudinally, so that the X-ray detector 50 is not located at any position between the sample 70 to be imaged and the luminescence imaging module; the microcomputer system controls the filter set 30 to switch and select the filter with the required bandwidth; the microcomputer system controls the first dichroic mirror 22 to rotate to a position which forms an angle of 45 degrees with the vertical direction and faces the InGaAs camera 24; as shown in fig. 6, the extended light source 12-1 is turned on, the excitation light emitted by the extended light source 12-1 is reflected by the reflector 12-2 and enters the beam expander 12-3, the light beam expanded by the beam expander 12-3 is incident on the second dichroic mirror 12-4 and then reflected to irradiate the sample 70 to be imaged, the fluorescence emitted by the sample 70 to be imaged after being excited by the extended light source 12-1 passes through the second dichroic mirror 12-4 and then is filtered by the optical filter to remove background interference light, the fluorescence is collected by the objective lens 21 and enters the first dichroic mirror 22, the fluorescence is reflected by the first dichroic mirror 22 to the InGaAs camera 24 to be collected and processed, and then the data is transmitted to the microcomputer system for image output.
The key points of the invention are as follows:
1. constructing an X-ray near-infrared two-region luminescence imaging analysis system (as shown in figures 2 and 3);
2. realizing high-sensitivity and broadband response (visible-near infrared one/two region, 400-;
3. quantitative dynamic X-ray luminescence imaging techniques.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (7)
1. An X-ray near-infrared two-zone luminescence bio-imaging device, comprising: the device comprises a light source module and a light-emitting imaging module;
the light emitting imaging module includes: an objective lens (21), a first dichroic mirror (22), a CCD camera (23) and an InGaAs camera (24);
the light source module is used for providing exciting light for a sample (70) to be imaged, the objective lens (21) is used for collecting fluorescence emitted by the sample (70) to be imaged after being excited, and the first dichroic mirror (22) is used for reflecting the fluorescence collected by the objective lens (21) to a light inlet of the CCD camera (23) or the InGaAs camera (24).
2. The X-ray near-infrared two-zone luminescence bio-imaging device according to claim 1, wherein the light source module comprises: an X-ray tube (11) for providing an X-ray source for the sample (70) to be imaged.
3. The X-ray near-infrared two-zone luminescence bio-imaging device of claim 2, wherein the light source module further comprises: an extended light source module for providing extended excitation light for the sample (70) to be imaged.
4. The X-ray near-infrared two-zone luminescence bio-imaging device according to claim 3, wherein the extended light source module comprises: the device comprises an extended light source (12-1), a reflector (12-2), a beam expander (12-3) and a second dichroic mirror (12-4);
the reflector (12-2) is used for reflecting the light source emitted by the extended light source (12-1) to the light inlet of the beam expander (12-3), and the second dichroic mirror (12-4) is used for reflecting the light source coming out of the light outlet of the beam expander (12-3) to the surface of the sample (70) to be imaged.
5. The X-ray near-infrared two-zone luminescence bio-imaging device of claim 1, further comprising: a filter set (30) for filtering fluorescence emitted by the sample (70) to be imaged after excitation.
6. The X-ray near-infrared two-zone luminescence bio-imaging device of claim 5, wherein the filter set (30) comprises: a filter set (30) disposed between the light source module and the objective lens (21).
7. The X-ray near-infrared two-zone luminescence bio-imaging device of claim 1, further comprising: an X-ray detector (50) for detecting fluorescence emitted by the sample (70) to be imaged after being excited by X-rays.
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| CN112577983A (en) * | 2020-12-18 | 2021-03-30 | 福州大学 | X-ray luminescence spectrum analysis system |
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