CN201108819Y - Accurate high-tension pulse static micro-capsule preparing instrument - Google Patents
Accurate high-tension pulse static micro-capsule preparing instrument Download PDFInfo
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- CN201108819Y CN201108819Y CNU2007200147158U CN200720014715U CN201108819Y CN 201108819 Y CN201108819 Y CN 201108819Y CN U2007200147158 U CNU2007200147158 U CN U2007200147158U CN 200720014715 U CN200720014715 U CN 200720014715U CN 201108819 Y CN201108819 Y CN 201108819Y
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- voltage pulse
- circuit
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- microcapsules
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Abstract
The utility model relates to a precise high pressure pulse electrostatic type microcapsule preparation instrument. An electric field is formed between the positive electrode of the nozzle vessel orifice needle and the negative electrode of the orifice in the precise high pressure pulse electrostatic type microcapsule preparation system; a high pressure pulse presser transformer; the lower end of the primary coil thereof of which is connected with the ground though an electronic switch, and the upper end is connected with a pulse power supply by a RC current limiting compensation net; the electronic switch is controlled by a voltage/frequency converter by a command signal output by a wave treatment driver; the secondary coil of the high pressure pulse presser transformer outputs high pressure pseudo-square wave used for preparing microcapsules by the diode which is connected positively. The diameter of the microcapsules prepared by the microcapsule preparation instrument can be controlled precisely and is even, the adjustment range is wide, the monodispersity is strong, fragments are few and material utilization ratio is high; the preparation and operation condition can be easily controlled and is stable, repeated and reliable, the instrument can feed and produce continuously, which improves the production ability and protects the biological activity of the products.
Description
Technical field
The utility model relates in a kind of biomedical sector the device of preparation microcapsules, specifically a kind ofly makes the electrostatic microcapsules preparing instrument of accurate high-voltage pulse that particle diameter is controlled, smooth surface is mellow and full.
Background technology
Microcapsules are the Perfected process that solves immunological rejection as cell transplantation immunity isolating tool, will become the new century to treat the new way of numerous diseases and receive much concern.It has been widely used in cell and has cultivated as a kind of extremely promising carrier, medicine controlled releasing and screening, the research and the production of biomedicine such as artificial organs, cell and enzyme immobilization and gene delivery vehicle, organizational project and pharmaceutical field.
But, the key issue of the restriction microcapsules technology research and the work of application is, still do not have a kind of comparatively ideal microcapsules moulding preparation facilities at present, thereby become the bottleneck that each colleges and universities of retardance China, R﹠D institution and medical institutions carry out the research and experiment work of this frontier.
Usually the orifice extruding or the pneumatic sessile drop method prepared microcapsule that adopt, because it is not of uniform size, the particle diameter inequality, out-of-shape, rough, sphericity and monodispersity are poor, thereby have limited extensive use.From the nineties beginning in last century, there are minority colleges and universities and research institution high voltage electrostatic technique to occur utilizing abroad, the experimental provision of development assembling microcapsules apparatus for preparation is significantly improved the quality of microcapsules preparation voluntarily.But often because its instrument of assembling is too simple and crude, various electrical quantitys are regulated difficulty even uncontrollable, also are that scope is narrow even can regulate, and poor stability is difficult to repeat.Thereby the surface of microcapsule of preparation is coarse, and the ball-type degree is undesirable, and particle diameter is inhomogeneous, and monodispersity is poor, and output is extremely low.Present most of instrument remains at some and designs unreasonable part, as regulate rough, poor repeatability, reliable and stable inadequately etc.So can't satisfy needing of the new work of scientific research.In recent years, though external more existing producers provide commercialization microcapsules preparing instrument product.But mostly cost an arm and a leg, and be air-flowing type but not electrostatic, prepared particle diameter is all more than 400 μ m, and quality is good inadequately, and shape is not mellow and full, monodispersity difference etc.Therefore still can't satisfy the need of research work.
At present, the still space state almost aspect preparation of the micron order microcapsules research of particle diameter in the 10-100 mu m range both at home and abroad, particularly also not having can be controlled for the generation particle diameter and the high molecule microcapsule apparatus for preparation of size dimension homogeneous.
The utility model content
At the present situation that still lacks at present the microcapsules apparatus for preparation of function admirable both at home and abroad, the purpose of this utility model is to provide a kind of controlled and big or small homogeneous of particle diameter for preparing, good sphericity, the electrostatic microcapsules preparing instrument of accurate high-voltage pulse that smooth surface is mellow and full.
For achieving the above object, the technical solution adopted in the utility model is:
The electrostatic microcapsules preparing instrument of the accurate high-voltage pulse of the utility model, between the shower nozzle container orifice syringe needle positive electrode of the electrostatic microcapsules preparation system of high-voltage pulse and orifice plate negative electrode, form electric field, has the high-voltage pulse step-up transformer, the lower end of its primary coil is by electronic switch ground connection, and the upper end links to each other with the pulse power by RC current limliting compensating network; Electronic switch is by the command signal control of voltage/frequency converter through the output of waveform processing drive processes; Secondary coil by the high-voltage pulse step-up transformer is used for the high pressure plan of microcapsules preparation like square wave through the diode output of forward connection.
Described waveform processing driver comprises pulsewidth translation circuit, primary carrier generation circuit, narrow pulse generating circuit and second carrier wave and amplifying circuit, wherein the pulsewidth translation circuit carries out the output signal of voltage/frequency converter to export primary carrier generation circuit to after the conversion process, and the output signal of primary carrier generation circuit forms through narrow pulse generating circuit and second carrier wave and amplifying circuit and drives the control end that signal exports electronic switch to; Base synchronous circuit and carrier wave generation circuit when described primary carrier generation circuit comprises, the base synchronous circuit is made up of multistage NAND gate in the time of wherein, and input links to each other with the pulsewidth translation circuit; Carrier wave generation circuit is made up of oscillating circuit and peripheral resistance capacitance, base synchronous circuit control when oscillating circuit is accepted, and output links to each other with the input of narrow pulse generating circuit; Also be provided with auxiliary high-voltage DC power supply, be connected to the output of the secondary coil of high-voltage pulse step-up transformer by high back-pressure silicon stack; Output at the secondary coil of high-voltage pulse step-up transformer is connected to the high-voltage pulse accessory power outlet, it is installed in the rectangle lucite box body of a sealing, in this box body, also be provided with one from behind plate can screw in the micro-porous permeable cylinder that replaceable discolour silica gel drier is housed; The ferromagnetic core of described high-voltage pulse step-up transformer is the high frequency high power ferrite magnetic core; Diode is the diode with high back-pressure, fast restorability.
The beneficial effects of the utility model and advantage are as follows:
1. particle size control is accurate, and adjustable range is wide;
2. grain size homogeneous, monodispersity is strong;
3. fragment is few, the utilization rate of raw materials height;
4. good sphericity, smooth in appearance is mellow and full;
5. the preparation manipulation condition is controlled and stable the repetition reliably easily;
6. can continuous feed, continue to produce, improved production capacity, protected the biologically active of product.
Description of drawings
Fig. 1 is microcapsules preparation system figure;
Fig. 2 is the utility model theory diagram;
Fig. 3 is utility model voltage/frequency converter circuit figure;
Fig. 4 is the pulsewidth translation circuit figure of waveform processing driver;
Fig. 5 is the primary carrier generator circuit figure of waveform processing driver;
Fig. 6 is the narrow-pulse generator circuit diagram of waveform processing driver;
Fig. 7 is the second carrier wave and the amplification circuit diagram of waveform processing driver;
Fig. 8 is the operating frequency of the utility model voltage/frequency converter and the linear relationship chart of input voltage;
Fig. 9 is the linear relationship chart between the high voltage pulse output Vp-p and the pulse power;
Final high-voltage pulse output Vp-p waveform schematic diagram when Figure 10 is primary carrier frequency f 1A=3kHZ;
Figure 11-1 is that 200 μ m calcium alginate micro gel beads of the 1st embodiment preparation of the utility model amplify picture;
Figure 11-2 is that 400 μ m calcium alginate micro gel beads of the 2nd embodiment preparation of the utility model amplify picture;
Figure 11-3 is that 600 μ m calcium alginate micro gel beads of the 3rd embodiment preparation of the utility model amplify picture;
Figure 12-1 is 200 μ m calcium alginate micro gel bead monodispersity oscillograms of the 1st embodiment preparation of the utility model;
Figure 12-2 is 400 μ m calcium alginate micro gel bead monodispersity oscillograms of the 2nd embodiment preparation of the utility model;
Figure 12-3 is 600 μ m calcium alginate micro gel bead monodispersity oscillograms of the 3rd embodiment preparation of the utility model.
The specific embodiment
Below in conjunction with accompanying drawing the utility model is described in further detail.
As shown in Figure 1, 2, the utility model is located in the electrostatic microcapsules preparation system of high-voltage pulse, forms electric field between its shower nozzle container orifice syringe needle positive electrode and orifice plate negative electrode, has high-voltage pulse step-up transformer T, its primary coil N
1The lower end by electronic switch K ground connection, the upper end links to each other with the pulse power by RC current limliting compensating network; Electronic switch K is by the command signal control of voltage/frequency converter through the output of waveform processing drive processes; Secondary coil N by high-voltage pulse step-up transformer T
2Diode D through the forward connection
0Output is used for the high pressure of microcapsules preparation to be intended like square wave, and its waveform parameter is operating frequency f
0, pulsewidth Δ T, high-voltage pulse peak-to-peak value amplitude V
P-P, baseline DC voltage E
AuxilliarySecondary coil N at high-voltage pulse step-up transformer T
2Output be diode D
0Negative electrode be connected to high-voltage pulse accessory power outlet CZOIA-CZOIC, it is installed in the rectangle lucite box body of a sealing, in this box body, also be provided with one from behind plate can screw in the micro-porous permeable cylinder that replaceable discolour silica gel drier is housed, be drying regime to keep in the lucite box body.
Accurate air constant pressure source in the electrostatic microcapsules preparation system of high-voltage pulse makes as the air pressure in flow process pipeline internally-powered source stable, utilize air pressure that the microcapsules core solution material flow in the shower nozzle container is passed through the orifice syringe needle consistently, and forcing under the effect of electric field, overcome the microballoon drop that surface tension forms the awl jet-like, fall into interior curing of liquid of gatherer.
Described high-voltage pulse step-up transformer T adopts with the ferrite ferromagnetic core as the high frequency high power ferrite magnetic core.
Present embodiment mesohigh pulse booster transformer T is the high-voltage pulse generator that center construction becomes.
Operating frequency fo is formed by the voltage/frequency converter, input voltage between from 0.00v to 3.50v can become the operating frequency fo output of 0-350.0HZ, its correspondence linear splendid, the linear relationship of the output of voltage/frequency converter and input signal as shown in Figure 8.
As shown in Figure 3, be voltage/frequency converter circuit schematic diagram, its input voltage is regulated by the ten circle helical potentiometer W01 that have the digital calibration dish, so frequency adjustment is very fine.Its operating frequency fo shows with the frequency meter panel table of 0-999.9HZ, thereby frequency values is regulated also very accurate.
As shown in Figure 4, be the pulsewidth translation circuit, each pulse width Δ T of operating frequency fo realizes 1ms～6ms pulse-width regulated by a band switch, is provided with 6 band switches in this enforcement.
Pulse width signal after the conversion carries out waveform processing through primary carrier generator as shown in Figure 5, makes the square wave of its every millisecond pulsewidth be subjected to the primary carrier frequency f
1A=1,2 or the modulation of 3KHZ.Further its pulsewidth is compressed through the primary carrier modulated waveform, and used the second carrier wave frequency f with narrow-pulse generator
2=50～60KHZ modulates, to satisfy the frequency of optimum traffic of ferrite high-voltage pulse step-up transformer T magnetic core, so that the plan that becomes top flat after boosting through high-voltage pulse step-up transformer T makes the jet particle stressed more even like square wave, thereby improves the quality of microcapsules preparation.
The final command signal that the process power drive is amplified is in order to open and close electronic switch K, and electronic switch K is arranged on the primary coil N of high-voltage pulse step-up transformer T
1Between lower end and the ground wire.Primary coil N
1The upper end links to each other with the high-voltage pulse power supply by RC current limliting compensating network, utilizes electronic switch K to reach and makes transformer N
1The purpose of command signal wave period ground and earth short or open circuit can be pressed in the lower end, thereby obtains the original alternating signal source of boosting.Through the transformer step-up coil is to become high voltage after secondary coil N2 boosts, again through having the diode Do rectification of high back-pressure, fast restorability, becoming the peak-to-peak value amplitude is the high direct voltage pulse output waveform of Vp-p, its amplitude can utilize regulating impulse power source voltage size to change, and can also utilize the indication panel digital watch of the pulse power that shows pulse power voltage E+ to come the high-voltage pulse peak-to-peak value of corresponding final output.Pulse power voltage E+ adjustable range can almost change to 100v from 0v, utilizes inferior/primary coil N of high-voltage pulse step-up transformer T
2/ N
1Step-up ratio, can make the Vp-p peak-to-peak value of high pressure output pulse change to ten from tens of volts surplus kilovolt.Present good linear relationship therebetween, as shown in Figure 9.
The magnetic core of high-voltage pulse step-up transformer T is made of ferrite.Its primary coil N
1With secondary coil N
2All be sealed in the tubular box body, and use epoxy sealing, moistureproof and anti-spark ability is strong.Primary coil N
1Have a plurality of centre caps, so that obtain optimum Working by debugging.Secondary coil N
2Be divided into three sections coilings, every section respectively with the diode D with high back-pressure, fast restorability
0Carry out rectification, thereby reduced the influence of distribution capacity, improved the quality of output high-voltage pulse waveform.
Because high-voltage pulse step-up transformer T adopts powerful FERRITE CORE, so have more high-power fan-out capability.Output adopts three special high-pressure accessory power outlet CZ01A～CZ01C that make with polytetrafluoroethylene (PTFE), can supply with more than one electrode assembly and prepare microcapsules, also can utilize one of them accessory power outlet and adopt oscillograph as the output waveform surveillance, monitor the microcapsules preparation quality by high pressure decay probe.
Except adopting high-voltage pulse, the auxiliary high-voltage DC power supply that the utility model has also increased by one group of 0-8KV in addition provides additional high-voltage electrostatic field.This high-voltage electrostatic field provides constant pulling force to the electrode shower nozzle, thereby can reduce the perturbed force that produces with high-voltage pulse widely, thereby further improves the quality of microcapsules preparation.This auxiliary DC high-voltage power supply voltage E
AuxilliaryBy high back-pressure silicon stack D
2Be added on high pressure accessory power outlet CZ01A～CZ01C of high-voltage pulse step-up transformer T.
Influenced by ambient humidity for fear of high pressure accessory power outlet CZ01A～CZ01C, change and cause exporting the pulse instability, even cause bad phenomenon such as the spark discharge of high-voltage pulse or corona discharge, be installed in the lucite box body of a sealing at high pressure accessory power outlet CZ01A～CZ01C high-voltage pulse accessory power outlet and attachment device thereof.The box body of a columnar replaceable discolour silica gel drier is set in this square lucite box again, and air is a drying regime in the lucite box body to keep.
As shown in figure 10, be as can be seen a kind of its envelope very near the plan of square waveform like the square-wave pulse sequence, its condition of work is: f
0=40.0HZ, Δ T=6ms, E+=40.0V, V
P-p=4.48kv, the primary carrier frequency f
1A=3KHZ.
The electrostatic microcapsules preparing instrument of accurate high voltage pulse is by regulating the calcium alginate plastic beads that the different operating parameter can prepare 10-4000 μ m.
Sodium alginate is dispersed in the sodium alginate material liquid that forms 1.5% (w/v) in 0.9% the physiological saline; The CaCl of 1.11% (w/v)
2Solution is coagulation bath, at high-voltage pulse E
+=88.8V, operating frequency fo=154Hz, pulsewidth Δ T=1ms, auxiliary high-voltage DC power supply voltage E
AuxilliaryUnder the situation of=1800V, the sodium alginate material liquid is that the speed of 2.03ml/h flows out from orifice with the flow, falling into coagulation bath solidifies, form the calcium alginate micro gel bead, shown in Figure 11-1,12-1, the calcium alginate micro gel bead that is respectively diameter with the utility model preparation and is 200 μ m amplifies 100 picture and with 200 μ m calcium alginate micro gel bead monodispersity oscillograms of the utility model preparation.
Experimental result shows, in the electrostatic microcapsules preparing instrument of the accurate high voltage pulse of the utility model institute tool range of operating parameters, select the prepared glueballs of operating parameter such as suitable pulse voltage, auxiliary high-voltage DC power supply voltage, operating frequency, flow to observe good sphericity through inverted microscope, the ball any surface finish, particle diameter and the particle diameter distribution laser particle analyzer of measuring microballoon through laser particle analyzer (U.S. LS-100Q of Beckman-Coulter company type) detect uniform particle diameter, and monodispersity is good.
Be that with the difference of embodiment 1 sodium alginate is dispersed in the sodium alginate material liquid that forms 1.5% (w/v) in 0.9% the physiological saline; The CaCl of 1.11% (w/v)
2Solution is coagulation bath, at pulse power voltage E+=47.8V, and operating frequency fo=123Hz, pulsewidth Δ T=6ms, auxiliary high-voltage DC power supply voltage E
AuxilliaryUnder the situation of=505V, the sodium alginate material liquid is that the speed of 7.67ml/h flows out from orifice with the flow, falls into coagulation bath and solidifies, and forms the calcium alginate micro gel bead.Shown in Figure 11-2,12-2, the calcium alginate micro gel bead that is respectively diameter with the utility model preparation and is 400 μ m amplifies 100 picture and with 400 μ m calcium alginate micro gel bead monodispersity oscillograms of the utility model preparation.
Be that with the difference of embodiment 1 sodium alginate is dispersed in the sodium alginate material liquid that forms 1.5% (w/v) in 0.9% the physiological saline; The CaCl of 1.11% (w/v)
2Solution is coagulation bath, at pulse power voltage E+=40V, and operating frequency fo=80Hz, pulsewidth Δ T=4ms, auxiliary high-voltage DC power supply voltage E
AuxilliaryUnder the situation of=505V, the sodium alginate material liquid is that the speed of 8.9ml/h flows out from orifice with the flow, falls into coagulation bath and solidifies, and forms the calcium alginate micro gel bead.Shown in Figure 11-3,12-3, the calcium alginate micro gel bead that is respectively diameter with the utility model preparation and is 600 μ m amplifies 100 picture and with 600 μ m calcium alginate micro gel bead monodispersity oscillograms of the utility model preparation.
Claims (7)
1. electrostatic microcapsules preparing instrument of accurate high-voltage pulse, between the shower nozzle container orifice syringe needle positive electrode of the electrostatic microcapsules preparation system of high-voltage pulse and orifice plate negative electrode, form electric field, it is characterized in that: have high-voltage pulse step-up transformer (T), its primary coil (N
1) the lower end by electronic switch (K) ground connection, the upper end links to each other with the pulse power by RC current limliting compensating network; Electronic switch (K) is by the command signal control of voltage/frequency converter through the output of waveform processing drive processes; Secondary coil (N by high-voltage pulse step-up transformer (T)
2) diode (D that connects through forward
0) the output high pressure that is used for microcapsules preparations intends like square wave.
2. according to the electrostatic microcapsules preparing instrument of the described accurate high-voltage pulse of claim 1, it is characterized in that: described waveform processing driver comprises pulsewidth translation circuit, primary carrier generation circuit, narrow pulse generating circuit and second carrier wave and amplifying circuit, wherein the pulsewidth translation circuit carries out the output signal of voltage/frequency converter to export primary carrier generation circuit to after the conversion process, and the output signal of primary carrier generation circuit forms through narrow pulse generating circuit and second carrier wave and amplifying circuit and drives the control end that signal exports electronic switch (K) to.
3. according to the electrostatic microcapsules preparing instrument of the described accurate high-voltage pulse of claim 2, it is characterized in that: base synchronous circuit and carrier wave generation circuit when described primary carrier generation circuit comprises, the base synchronous circuit is made up of multistage NAND gate in the time of wherein, and input links to each other with the pulsewidth translation circuit; Carrier wave generation circuit is made up of oscillating circuit and peripheral resistance capacitance, base synchronous circuit control when oscillating circuit is accepted, and output links to each other with the input of narrow pulse generating circuit.
4. according to the electrostatic microcapsules preparing instrument of the described accurate high-voltage pulse of claim 1, it is characterized in that: also be provided with auxiliary high-voltage DC power supply, by high back-pressure silicon stack (D
2) be connected to the secondary coil (N of high-voltage pulse step-up transformer (T)
2) output.
5. according to the electrostatic microcapsules preparing instrument of the described accurate high-voltage pulse of claim 1, it is characterized in that: at the secondary coil (N of high-voltage pulse step-up transformer (T)
2) output be connected to high-voltage pulse accessory power outlet (CZOIA-CZOIC), it is installed in the rectangle lucite box body of a sealing, in this box body, also be provided with one from behind plate can screw in the micro-porous permeable cylinder that replaceable discolour silica gel drier is housed.
6. according to the electrostatic microcapsules preparing instrument of the described accurate high-voltage pulse of claim 1, it is characterized in that: the ferromagnetic core of described high-voltage pulse step-up transformer (T) is the high frequency high power ferrite magnetic core.
7. according to the electrostatic microcapsules preparing instrument of the described accurate high-voltage pulse of claim 1, it is characterized in that: diode (D
0) for having the diode of high back-pressure, fast restorability.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200147158U CN201108819Y (en) | 2007-09-26 | 2007-09-26 | Accurate high-tension pulse static micro-capsule preparing instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200147158U CN201108819Y (en) | 2007-09-26 | 2007-09-26 | Accurate high-tension pulse static micro-capsule preparing instrument |
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| Publication Number | Publication Date |
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| CN201108819Y true CN201108819Y (en) | 2008-09-03 |
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ID=39893335
Family Applications (1)
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|---|---|---|---|
| CNU2007200147158U Expired - Lifetime CN201108819Y (en) | 2007-09-26 | 2007-09-26 | Accurate high-tension pulse static micro-capsule preparing instrument |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178604A (en) * | 2011-03-22 | 2011-09-14 | 中国人民解放军第三○九医院 | Device and method for preparing gel microsphere and gel microsphere in which antitubercular agents can be injected |
| CN104324676A (en) * | 2014-11-14 | 2015-02-04 | 上海理工大学 | Microcapsule generation device |
| WO2015085898A1 (en) * | 2013-12-09 | 2015-06-18 | The University Of Hong Kong | Core-shell capsules for encapsulation of particles, colloids, and cells |
| CN107667296A (en) * | 2015-06-17 | 2018-02-06 | 英特尔Ip公司 | It is used for electrostatic test in orientation impulses injection microelectronics system |
-
2007
- 2007-09-26 CN CNU2007200147158U patent/CN201108819Y/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178604A (en) * | 2011-03-22 | 2011-09-14 | 中国人民解放军第三○九医院 | Device and method for preparing gel microsphere and gel microsphere in which antitubercular agents can be injected |
| CN102178604B (en) * | 2011-03-22 | 2014-03-05 | 中国人民解放军第三0九医院 | Device and method for preparing gel microsphere and gel microsphere in which antitubercular agents can be injected |
| WO2015085898A1 (en) * | 2013-12-09 | 2015-06-18 | The University Of Hong Kong | Core-shell capsules for encapsulation of particles, colloids, and cells |
| CN105979938A (en) * | 2013-12-09 | 2016-09-28 | 香港大学 | Core-shell capsules for encapsulation of particles, colloids, and cells |
| CN104324676A (en) * | 2014-11-14 | 2015-02-04 | 上海理工大学 | Microcapsule generation device |
| CN107667296A (en) * | 2015-06-17 | 2018-02-06 | 英特尔Ip公司 | It is used for electrostatic test in orientation impulses injection microelectronics system |
| CN107667296B (en) * | 2015-06-17 | 2021-01-05 | 英特尔Ip公司 | Directional pulse injection in microelectronic systems for electrostatic testing |
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|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20080903 |