CN201072391Y

CN201072391Y - Automatic objective separation device based on immune magnetic beads

Info

Publication number: CN201072391Y
Application number: CNU2007201129195U
Authority: CN
Inventors: 叶尊忠; 应义斌; 王剑平; 李延斌; 饶秀勤; 蒋焕煜; 蒋雪松
Original assignee: Zhejiang University ZJU
Current assignee: Zhejiang University ZJU
Priority date: 2007-08-06
Filing date: 2007-08-06
Publication date: 2008-06-11
Anticipated expiration: 2017-08-06

Abstract

The utility model discloses an automatic separation device which is based on immunomagnetic beads and comprises a mixing unit, a separating unit and a control unit. Specimen to be tested and the immunomagnetic beads are added to a sample tube which later rotates evenly, at variable speed, statically or backwards and forwards and antibodies which are stable on objects of the specimen and the immunomagnetic beads in the sample tube act distinctively; the immunomagnetic beads and the objects in the specimen and captured by the immunomagnetic beads are absorbed to the inner wall of the sample tube in fixed magnetic field; then the immunomagnetic beads and the objects in the specimen and captured by the immunomagnetic beads are used for biological detection or traying. Through special reaction of the stable antibodies on the objects of the specimen and the immunomagnetic beads in the sample tube, the objective detection matters is accurately separated and screened and the optimized mixing effect is realized through the package of different mixing modes, therefore, the utility model is widely applied in the pretreatment of specimens.

Description

A kind of target automatic separating apparatus based on immunomagnetic beads

Technical field

The utility model relates to a kind of target automatic separating apparatus based on immunomagnetic beads.

Background technology

As everyone knows, sample pre-treatments is very important means, and its success or not is most important to the detection of sample.Utilize immune magnetic particle (magnetic bead) to screen and be separated in food, clinical, soil obtains more and more certainly with the method for targets such as the microorganism in the environment, cell, chemical element.Owing to quick, the highly enriched ratio of immunomagnetic beads separation/screening technique, to advantages such as equipment requirements are simple, use very wide in practice.At present, immunomagnetic beads separation/screening technique generally is made up of two steps: mix and separate.General mixer can only be by the rotation of direction, and rotational speed is manual adjustments, and incorporation time can only be by artificial control; Separation equipment generally is made up of permanent magnet and support, and disengaging time also is by manually controlling.Because these two committed steps are independent operations, make separating effect be subjected to the influence of all many-sides, and must buy a plurality of equipment and just can finish the first separation operation, these factors make the pre-treatment effect be subjected to very big influence.

Summary of the invention

The utility model provides a kind of target automatic separating apparatus based on immunomagnetic beads.

The technical solution adopted in the utility model is as follows: comprise mixed cell, separative element and control module; Wherein:

1) mixed cell, n sample hose are separately fixed on the biased sample plate in separately the sample well, and hybrid electric motor is rotationally connected by first shaft joint and biased sample plate; Hybrid electric motor is electrically connected by umbilical cable and control module;

2) separative element, n piece discrete magnets are separately fixed at and separate on the driver plate, n piece discrete magnets be fixed on the mixed drive plate on n sample hose corresponding one by one, the separation motor by second shaft joint with separate driver plate and be rotationally connected; Separating motor is electrically connected by umbilical cable and control module;

3) control module, single-chip microcomputer link to each other with LCD, keyboard, buffering drive circuit, crystal oscillating circuit, parameter storage, communication interface, alarm unit respectively; Buffering drive circuit connects hybrid electric motor respectively and separates motor; Communication interface links to each other with computing machine through usb communication line, power circuit respectively with power supply input, single-chip microcomputer, separate motor and link to each other with hybrid electric motor.

The X1 of AT89C52 single-chip microcomputer, X2 mouth are connected with crystal oscillating circuit; The P0 mouth of single-chip microcomputer and the buffering drive circuit of forming by 74HC573 chip and ULN2803A chip respectively with hybrid electric motor with separate motor and link to each other;

The P2 mouth of single-chip microcomputer links to each other with the GDM12864 LCD by extended chip 74HC595 with P3.4, P3.6, P3.7; The P1 mouth of single-chip microcomputer links to each other with 4 * 4 keyboards; The RXD of single-chip microcomputer, TXD mouth link to each other with communication interface; The P3.2 of single-chip microcomputer, P3.3 mouth link to each other with parameter storage; The P3.5 mouth of single-chip microcomputer links to each other with alarm unit.

The beneficial effect that the utlity model has is: produce specific reaction by antibody fixing on the detected target in the sample hose and the immunomagnetic beads and can accurately separate and screen the target detection thing, and the combination by the different blended syntype reaches optimum mixed effect, is widely used in the pre-treatment of sample.

Description of drawings

Fig. 1 is the overall schematic of tripping device.

Fig. 2 is that the integral body of tripping device and control enclosure and sample mix plate revolve the synoptic diagram that turn 90 degrees.

Fig. 3 is that the integral body of tripping device is formed structural drawing.

Fig. 4 is the control circuit composition diagram of tripping device.

Fig. 5 is the control circuit schematic diagram of tripping device.

Among the figure: 1, mixed cell; 2, separative element; 3, control module; 101,102,103,104, sample hose; 105,106,107,108, sample well; 109, hybrid electric motor; 110, bearing; 111, biased sample plate; 112, shaft joint; 201,202,203,204, discrete magnets; 205, separate driver plate; 206, bearing; 207, shaft joint; 208, separate motor; 301,302, insulated support; 303, umbilical cable; 304, LCD; 305, keyboard; 306, control circuit board; 307, buffering drive circuit; 308, power supply input; 309, power circuit; 310, single-chip microcomputer; 311, crystal oscillating circuit; 312, parameter storage; 313, communication interface; 314, alarm unit; 315, usb communication line; 316, computing machine.

Embodiment

The utility model is described in further detail below in conjunction with drawings and Examples.

As Fig. 1, Fig. 2, Fig. 3, shown in Figure 4, the utility model comprises mixed cell 1, separative element 2 and control module 3; Wherein:

1) mixed

cell

1,4 sample hoses 101,102,103,104, be separately fixed on the biased sample plate 111 in 105,106,107,108 sample wells separately, one end of biased sample plate 111 links to each other by first shaft joint 112 with hybrid electric motor 109, and the other end of biased sample plate 111 links to each other with shell by bearing 110; Hybrid electric motor 109 is electrically connected by umbilical cable 303 and control module 3;

2)

separative element

2,4 discrete magnets are separately fixed at separates on the

driver plate

205,4 discrete magnets be fixed on biased sample plate 111 on 4 sample hoses corresponding one by one, an end that separates driver plate 205 by shaft joint 207 with separate motor 208 and link to each other, the other end that separates driver plate 205 links to each other with shell by bearing 206; Separating motor 208 is electrically connected by umbilical cable 303 and control module 3;

3) control module 3, and single-chip microcomputer 310 links to each other with LCD 304, keyboard 305, buffering drive circuit 307, crystal oscillating circuit 311, parameter storage 312, communication interface 313, alarm unit 314 respectively; Buffering drive circuit 307 connects hybrid electric motor 109 respectively and separates motor 208; Communication interface 313 links to each other with computing machine 316 through usb communication line 315, power circuit 309 respectively with power supply input 308, single-chip microcomputer 310, separate motor 208 and link to each other with hybrid electric motor 109, control circuit board 306 usefulness 301 and 302 insulated supports rest on the housing.

As shown in Figure 5, the X1 of AT89C52 single-chip microcomputer 310, X2 mouth are connected with crystal oscillating circuit 311; The P0 mouth of single-chip microcomputer 310 and the buffering drive circuit of forming by 74HC573 chip and ULN2803A chip 307 respectively with hybrid electric motor 109 with separate motor 208 and link to each other; The P2 mouth of single-chip microcomputer 310 links to each other with GDM12864 LCD 304 by extended chip 74HC595 with P3.4, P3.6, P3.7; The P1 mouth of single-chip microcomputer 310 links to each other with 4 * 4 keyboards 305; The RXD of single-chip microcomputer 310, TXD mouth link to each other with communication interface 313; The P3.2 of single-chip microcomputer 310, P3.3 mouth link to each other with parameter storage 312; The P3.5 mouth of single-chip microcomputer 310 links to each other with alarm unit 314.LCD 304, it can show English character and Chinese character.

Detected sample and immunomagnetic beads are joined in the sample hose, sample hose is under the various combination of at the uniform velocity rotation, speed change rotation, static enrichment or forward and reverse rotation status, and antibody fixing on target in the detected sample in the sample hose and the immunomagnetic beads produces specific reaction; Under fixed magnetic field, immunomagnetic beads be adsorbed on the sample hose inwall, draw the damping fluid that cleans sample hose after the supernatant and add small size by the target in the immune magnetic detected sample captured; Collect immunomagnetic beads and, be used for biological detection or shop dish by the target in the immune magnetic detected sample captured.

The course of work of the present utility model is as follows:

(1) immunomagnetic beads and detected sample are joined in the sample hose 101,102,103,104, and close the cover;

(2) sample hose 101,102,103,104 is inserted in the sample well 105,106,107,108;

(3) import parameters such as incorporation time, disengaging time and mixed mode on keyboard 305, LCD 304 will show occurrence; After input finishes, the start button on the keypad 305;

(4) control circuit board 306 output signals control hybrid electric motor 109 drives and mixes biased sample plate 111 and drive sample hose 101,102,103,104 by the pattern rotation of setting; Rotatablely move and make immunomagnetic beads and the interior object of detected sample in the sample hose 101,102,103,104 fully mix;

(5) after blend step finishes, control circuit board 306 output signals make sample hose 101,102,103,104 be in initial upright position, the control of control circuit board 306 output signals separates motor 208, drive the fixing discrete magnets 201,202,203,204 onboard of separation driver plate 205 and drive and arrive the outer wall of sample hose 101,102,103,104, and stop operating; Under the action of a magnetic field of discrete magnets 201,202,203,204, separated and assembling near magnetic direction toward the inwall of sample hose 101,102,103,104 gradually by the object in the immunomagnetic beads institute detected sample captured, and begin agglomerating with solution;

(6) after step to be separated finished, the operator opened the lid of sample hose 101,102,103,104 and takes supernatant away with liquid-transfering gun; After taking supernatant away, the conclusion button on the keypad 305;

(7) control of control circuit board 306 output signals separates motor 208, drives to separate driver plate 205 and drive the outer wall that discrete magnets 201,202,203,204 is left sample hose 101,102,103,104; And return to initial position;

(8) take off in the sample hose 101,102,103,104, the damping fluid that adds small size just can obtain by immunomagnetic beads captured object thing solution.Can obtain the sample after the concentrating and separating like this, it can be used for subsequent detection such as biology sensor detection, ELISA, PCR, for they provide very effective sample and processing means, and has improved detectability.

Fig. 4 is the control circuit composition diagram of tripping device.In order to make computing machine 316 control magnetic separating devices, magnetic separating device is connected with computing machine 316 by usb communication line 315.The operation of computer software control magnetic separating device, and monitor the state of magnetic separating device in real time, make things convenient for user management.

Above-mentioned embodiment is used for the utility model of explaining; rather than the utility model limited; in the protection domain of spirit of the present utility model and claim, any modification and change to the utility model is made all fall into protection domain of the present utility model.

Claims

1. the target automatic separating apparatus based on immunomagnetic beads is characterized in that comprising mixed cell (1), separative element (2) and control module (3); Wherein:

1) mixed cell (1), n sample hose are separately fixed on the biased sample plate (111) in separately the sample well, and hybrid electric motor (109) is rotationally connected by first shaft joint (112) and biased sample plate (111); Hybrid electric motor (109) is electrically connected by umbilical cable (303) and control module (3);

2) separative element (2), n piece discrete magnets is separately fixed at separates on the driver plate (205), to go up n sample hose corresponding one by one with being fixed on biased sample plate (111) for n piece discrete magnets, separation motor (208) by second shaft joint (207) with separate driver plate (205) and be rotationally connected; Separating motor (208) is electrically connected by umbilical cable (303) and control module (3);

3) control module (3), single-chip microcomputer (310) link to each other with LCD (304), keyboard (305), buffering drive circuit (307), crystal oscillating circuit (311), parameter storage (312), communication interface (313), alarm unit (314) respectively; Buffering drive circuit (307) connects hybrid electric motor (109) respectively and separates motor (208); Communication interface (313) links to each other with computing machine (316) through usb communication line (315), power circuit (309) respectively with power supply input (308), single-chip microcomputer (310), separate motor (208) and link to each other with hybrid electric motor (109).

2. a kind of target automatic separating apparatus based on immunomagnetic beads according to claim 1 is characterized in that: the X1 of AT89C52 single-chip microcomputer (310), X2 mouth are connected with crystal oscillating circuit (311); The P0 mouth of single-chip microcomputer (310) and the buffering drive circuit of forming by 74HC573 chip and ULN2803A chip (307) respectively with hybrid electric motor (109) with separate motor (208) and link to each other; The P2 mouth of single-chip microcomputer (310) links to each other with GDM12864 LCD (304) by extended chip 74HC595 with P3.4, P3.6, P3.7; The P1 mouth of single-chip microcomputer (310) links to each other with 4 * 4 keyboards (305); The RXD of single-chip microcomputer (310), TXD mouth link to each other with communication interface (313); The P3.2 of single-chip microcomputer (310), P3.3 mouth link to each other with parameter storage (312); The P3.5 mouth of single-chip microcomputer (310) links to each other with alarm unit (314).