CN201010507Y

CN201010507Y - High-precision micropipette

Info

Publication number: CN201010507Y
Application number: CNU2007200626523U
Authority: CN
Inventors: 杜治坤; 聂前满; 郭仁虎
Original assignee: Individual
Current assignee: Individual
Priority date: 2007-03-07
Filing date: 2007-03-07
Publication date: 2008-01-23
Anticipated expiration: 2017-03-07

Abstract

A micro liquid adding machine with high accuracy used for analyzing and monitoring the added micro solution comprises a test tube (5) and a container for solution to be sucked (7), the upper port of the test tube (5) is equipped with a rubber plug (4). The micro liquid adding machine is characterized in that an air exhaust tube (3) passes through the rubber plug (4), and one end is communicated with the test tube (5) inner cavity, one end of a hydrophobic thin tube (1) passes the rubber plug (4) and the port is positioned in the test tube (5), the other end of the hydrophobic thin tube (1) is placed in the solution of container for solution to be sucked (7), the inner diameter of the hydrophobic thin tube (1) is less than or equal to 2 mm and the diameter of the two ports thereof is less than or equal to 0.1 mm, and a scratch liquid needle (2) passes the rubber plug (4) and the lower end is a bending structure. When the liquid adding amount is 100m3, the repeatable accuracy can reach 0.1 percent, thereby realizing the liquid on monitoring the materials to be measured with small size and high accuracy.

Description

High precision microscal burette

Technical field

The utility model relates to analysis and detection technology, further is meant the high precision liquid-adding device that is used to add trace reagent or sample solution.

Background technology

The liquid-adding device of prior art has conventional calibrated pipet, whole pipette, micropipet, automatic pipet(te), micro-precision pipet etc., wherein liquid absorption more than 1ml (1000 μ l) and repeatable accuracy can reach 0.5% have only the micro-precision pipettor; Liquid absorption is less than the repeatable accuracy of the micro-precision pipettor out of reach 0.5% of 1000 μ l.

The utility model content

The technical problems to be solved in the utility model is, the defective at prior art exists proposes a kind of high precision microscal burette, and when its enough hour of the amount of draw solution, its repeatable accuracy can reach below 0.1%.

Technical solution of the present utility model is, described high precision microscal burette comprises a test tube and solution container to be sucked, the upper port of described test tube is equipped with soft rubber ball, its architectural feature is, extraction pipe passes described soft rubber ball and the one end is communicated with described test tube inner chamber, one end of a hydrophobic tubule is arranged in test tube by described soft rubber ball and its port, the other end of this hydrophobic tubule places the solution of waiting to suck solution container, internal diameter≤the 2mm of described hydrophobic tubule and its two-port diameter all≤0.1mm, a scraping fluid pin is a bending structure by described soft rubber ball and its lower end.

Below the utility model made further specify.

As shown in Figure 1, the utility model comprises a test tube 5 and waits to suck solution container 7, the upper port of described test tube 5 is equipped with soft rubber ball 4, its architectural feature is, extraction pipe 3 passes described soft rubber ball 4 and the one end is communicated with described test tube 5 inner chambers, one end of a hydrophobic tubule 1 is arranged in test tube 5 by described soft rubber ball 4 and its port, the other end of this hydrophobic tubule 1 places the solution of waiting to suck solution container 7, internal diameter≤the 2mm of described hydrophobic tubule 1 and its two-port diameter all≤0.1mm, a scraping fluid pin 2 is a bending structure by described soft rubber ball 4 and its lower end.Also can the removal waste fluid mouth 6 that valve is housed be set in the bottom of test tube 5.

Described hydrophobic tubule is a liquid storage tube, and its length is set arbitrarily according to required liquid volume added.

During actual the use, the lower end insertion of described hydrophobic tubule 1 is waited to suck the solution 2-3mm degree of depth, bleed with extraction pipe 3 (connecing air pump), the solution to be sucked that wait to suck in the solution container 7 this moment enters in the hydrophobic tubule 1, and unnecessary liquid flows out from upper orifice; Stop to bleed, then 2-3 is after second, and hydrophobic tubule 1 reaches four kinds of forcees balance of inside and outside gaseous tension, liquid level difference gravity, surface tension, suction pipe resistance; Turn scraping fluid pin 2 this moment, and the unnecessary droplet that will rest on hydrophobic tubule 1 upper port (outlet) strikes off; Remove again and wait to suck solution container 7, hydrophobic tubule lower end is inserted receive ware,, just the liquid that quantitatively sucks in the hydrophobic tubule 1 is accurately discharged with extraction pipe 3 air inlets.

High precision microscal burette of the present utility model can use separately, also can a plurality ofly make up, simultaneously operation.

Know-why of the present utility model is, adopts the hydrophobic tubule, and it has smooth inwall and hydrophobic, when its with less than the speed discharge opeings of 2 μ l/ seconds the time, inner-walls of duct adheres to little of ignoring to the infiltration of solution; Because this suction pipe internal diameter of the pipeline is enough little, two-port is littler again, and after bleeding with extraction pipe 3,2-3 just can reach above-mentioned four kinds of equilibrium of forces after second; Because dynamic resistance is enough big, the degree of depth of described tubule insertion liquid level and insertion, taking-up can not influence the volume that sucks solution.As long as design-calculated chemical reaction flow process has enough accuracy rates, signal monitoring also has enough precision, and final detection error is reached below 0.1%.This high accuracy analysis for high content component has significant preceence; With the photometric analysis is example, and the colour developing volume can dwindle more than 100 times, and developer can be saved more than 95%, and multichannel is got liquid and liquid feeding simultaneously, can accomplish rapid, automation, can reach high-precision requirement again.

As known from the above, the utility model is a kind of high precision microscal burette, when the internal diameter of the hydrophobic tubule that adopts be about≤2mm and its two-port diameter all≤0.1mm, during the about 100mm of length, the about 100 μ l of volume, the volume of 1mm length is less than 0.1 μ l; When imbibition reaches balance, again with the scraping fluid pin scrape off that tip may retain more than droplet, the solution relative error of each injection tubule is controlled at below 0.1%.

Description of drawings

Fig. 1 is a kind of example structure scheme drawing of the present utility model.

In the drawings:

1-hydrophobic tubule, 2-scraping fluid pin, the 3-extraction pipe,

The 4-soft rubber ball, the 5-test tube, 6-removal waste fluid mouth,

7-solution to be sucked.

The specific embodiment

As shown in Figure 1, it comprises a test tube 5 and waits to suck solution container 7, the upper port of test tube 5 is equipped with sealed rubber plug 4, extraction pipe 3 passes soft rubber ball 4 and the one end places test tube 5, one end of a hydrophobic tubule 1 is arranged in test tube 5 by soft rubber ball 4 and its port, and the other end of this hydrophobic tubule places the solution of waiting to suck solution container 7, and described hydrophobic tubule 1 usefulness hydrophobic polytetrafluoroethylene or other hydrophobic material are made, internal diameter of the pipeline is 1mm, and the two-port bore is 0.02mm; When its draw solution amount was 100 μ l, 10 times repeatable accuracy was 0.1%; When the draw solution amount was 500 μ l, 10 times repeatable accuracy reached 0.03%; Can accomplish small size, the various test substances of high Precision Detection.

Claims

1. high precision microscal burette, comprise a test tube (5) and solution container to be sucked (7), the upper port of described test tube (5) is equipped with soft rubber ball (4), it is characterized in that, an extraction pipe (3) passes described soft rubber ball (4) and the one end is communicated with described test tube (5) inner chamber, one end of a hydrophobic tubule (1) is arranged in test tube (5) by described soft rubber ball (4) and its port, the other end of this hydrophobic tubule (1) places the solution of solution container to be sucked (7), internal diameter≤the 2mm of described hydrophobic tubule (1) and its two-port diameter all≤0.1mm, a scraping fluid pin (2) is a bending structure by described soft rubber ball (4) and its lower end.

2. according to the described high precision microscal burette of claim 1, during its feature, the removal waste fluid mouth (6) that valve is housed is set in the bottom of test tube (5).