CN200993623Y - Salt bath biological soft tissue tensile test fixture - Google Patents

Salt bath biological soft tissue tensile test fixture Download PDF

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Publication number
CN200993623Y
CN200993623Y CN 200620175985 CN200620175985U CN200993623Y CN 200993623 Y CN200993623 Y CN 200993623Y CN 200620175985 CN200620175985 CN 200620175985 CN 200620175985 U CN200620175985 U CN 200620175985U CN 200993623 Y CN200993623 Y CN 200993623Y
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CN
China
Prior art keywords
heat
metal tube
soft tissue
waterproof
chuck
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Expired - Fee Related
Application number
CN 200620175985
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Chinese (zh)
Inventor
欧阳云飞
杨柳
段小军
戴刚
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First Affiliated Hospital of TMMU
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First Affiliated Hospital of TMMU
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Priority to CN 200620175985 priority Critical patent/CN200993623Y/en
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Publication of CN200993623Y publication Critical patent/CN200993623Y/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

The utility model discloses a clamp of soft tissue tension test for brine-bathed biology, which comprises a lower clamp holder and a lower clamp holder and a constant temperature water tub. Wherein, the lower clamp holder is inserted from a bottom of the constant temperature water tub. The upper clamp holder is arranged through an upper part of the constant temperature water tub. A metal tube is respectively provided for the upper clamp holder and lower clamp holder and wrapped by a jacket. A cavity is positioned between the metal tube and the jacket. A refrigerating agent inlet tube and a refrigerating agent outlet tube are connected with the jacket. In addition, the jacket is sealed into a waterproof and heat-insulating coat. One end of the metal tube is positioned into the waterproof and heat-insulating coat. A thru hole is arranged at an end of the waterproof and heat-insulating coat to match a nozzle of the metal tube. A gasket ring is arranged between the metal tube and waterproof and heat-insulating coat. The other end of the coat is extended out of the coat through the refrigerating agent inlet and outlet tube. Heat insulator is stuffed or a vacuum space is formed between the waterproof heat-insulating coat and the jacket. The refrigerating clamp of soft tissue tension test for brine-bathed biology can be utilized under a simulating physiological environment (37 Celsius degrees of brine bathing) without damaging soft tissues.

Description

Brine bath biological soft tissue stretching test anchor clamps
Technical field
The utility model is with relating to a kind of instrument, is applied to the biomechanics test, is a kind of anchor clamps that can freezing clamping biologic soft tissue carry out the biomechanics test under 37 ℃ brine bath condition specifically.
Background technology
The biomechanics tester is made up of the last anchor clamps and the lower clamp that are installed on the mechanical test machine, and last lower clamp is used for the biological tissue two ends are clamped.Run into the problem that biologic soft tissue grips through regular meeting in biomechanics test, common anchor clamps are with to be easy to generate stress after soft tissue contacts concentrated and disorganize influences the mechanics accuracy of test.In the experimentation that obtains small soft tissue biomechanics data, the viscous-elastic behaviour of soft tissue softness is given to grip and is brought very big difficulty.Traditional clamp method all is to adopt hard metal fixture that piece of tissue is carried out clamp, and is also feasible for tissue such as bone that hardness is high, but just is difficult to for soft tissue such as muscle, tendon, ligament, skin or the like.Sample damage and slippage easily promptly allows to clamp in the process of clamping, concentrates but can form stress on soft tissue piece and anchor clamps surface of contact, and material is caused damage, influences the accuracy of test data.Catch test specimen and don't broken test specimen itself that how can firm stable be difficult problems in the biomechanics test always.
It is a kind of effective method that utilization Refrigeration Technique raising soft tissue hardness makes it to be integral with the anchor clamps strong bonded.Freezing clamping technology has the utilization of certain limit in the eighties of last century middle and later periods at industrial circle, but the utilization of biomechanics field is less.Since the nineties in last century, American-European developed country develops the device of several freezing clampings for this reason, employing be the electronics Refrigeration Technique, can make rapidly that to be held soft tissue freezing, but just be used for the extracting of soft tissue, be not applied to because of acting force is less in the biomechanics test.In recent years the bulk soft tissue that a kind of freezing anchor clamps (Tissue Grips-Thermal-Electrically Cooled) produced of the BOSE company of the U.S. can clamping 33mm * 19mm (wide, thick), maximum grip power can arrive 2250 newton.And strong unit of domestic several family can only rely on from this equipment of developed country's import and finishes experiment, and price is very expensive.In addition, this anchor clamps can only be used for clamping loading under the normal temperature environment, can not be used for simulating 37 ℃ of water-bath environment of physiological condition, therefore can not carry out the biologic soft tissue mechanical test under the simulation physiological condition.
The utility model content
The purpose of this utility model is to provide a kind of novel brine bath biological soft tissue stretching test anchor clamps, this freezing anchor clamps can grasp biologic soft tissue and can not produce pressure to soft tissue under 37 ℃ brine bath environment of simulation physiological condition, thereby can not damage soft tissue owing to clamping.
The utility model adopts following technical scheme: a kind of brine bath biological soft tissue stretching test anchor clamps, comprise the lower chuck and the upper grip that are installed on the mechanical test machine, it is characterized in that: also comprise a thermostatted water bathtub, lower chuck from described thermostatted water bath base stretch into and and the water bath with thermostatic control cylinder bottom between form sealing, described upper grip self-constant temperature cleansing bath tub top is stretched in the described thermostatted water bathtub and is relative with lower chuck; Described upper grip and lower chuck include a metal tube, metal tube is with chuck outward, the chamber that constitutes sealing between metal tube and the chuck forms evaporation tube and is used to feed cold-producing medium, being connected with refrigerant inlet pipe and refrigerant outlet pipe on the chuck communicates with described chamber, described chuck is encapsulated in the waterproof and heat-insulating overcoat, described metal tube one end is positioned at the waterproof and heat-insulating overcoat and has through hole relative with the mouth of pipe of this end of metal tube in waterproof and heat-insulating overcoat end, and this end has O-ring seal between metal tube and waterproof and heat-insulating overcoat, the described metal tube other end and refrigerant inlet pipe and refrigerant outlet pipe are piercing in outside this insulated sheath, be filled with insulation material or form vacuum between described waterproof and heat-insulating overcoat and described chuck.
This 37 ℃ brine bath biological soft tissue stretching test anchor clamps of the present utility model, comprise the evaporation tube that the outer chuck of a metal tube and metal tube forms, cold-producing medium with this evaporation tube during use advances, outlet is connected with refrigeration unit, make cavity between metal tube and the chuck as the evaporator of refrigeration unit, soft tissue pulled into utilize the evaporator strip of cold-producing medium in this evaporator to walk heat in the metal tube, make soft tissue and metal tube temperature reduce to subzero, moisture soft tissue can freeze to be bonded together with metal tube, reach the purpose that realizes grasping soft tissue, this fixed form does not apply clamping pressure to soft tissue, therefore also can guarantee the excellent of test specimen after thawing, overcome the deficiency that prior art causes soft tissue stress to concentrate.And owing to increased the waterproof and heat-insulating overcoat and formed the insulation layer, therefore this anchor clamps can also be used for 37 ℃ of water-bath environment, can under the simulation physiological condition, experimentize, and be that prior art is incomparable.
Description of drawings
Fig. 1 is the structural representation of a kind of biological soft tissue stretching test of the present utility model with freezing lower chuck.
Fig. 2 is the structural representation of a kind of biological soft tissue stretching test of the present utility model with freezing upper grip.
Fig. 3 is the assembling synoptic diagram that 37 ℃ brine bath biological soft tissue stretching test is used anchor clamps.
Embodiment
Below in conjunction with the drawings and specific embodiments brine bath biological soft tissue stretching test anchor clamps of the present utility model are described further.
As shown in Figure 3,37 ℃ brine bath biological soft tissue stretching test anchor clamps, comprise the lower chuck 27 and the upper grip 21 that are installed on the mechanical test machine 35, and thermostatted water bathtub 22, lower chuck 27 self-constant temperature cleansing bath tubs 22 bottoms stretch into and and the end of thermostatted water bathtub 22 between form sealing, to prevent the salt solution seepage, cleansing bath tub 22 bottoms communicate with the clamping hole 1 of lower chuck 27 simultaneously, and rubber sealing is arranged between the two.It is interior and relative with lower chuck 27 that described thermostatted water bathtub is stretched in upper grip 21 self-constant temperature cleansing bath tubs 22 tops.The mechanical test machine is an existing product, and its structure need not to describe at this.Upper grip and lower chuck adopt the freezing clamp of waterproof and heat-insulating.
The lower chuck structure as shown in Figure 1, the upper grip structure as shown in Figure 2, its agent structure basically identical.Include the freezing grip tube that a metal tube 4 constitutes, be with a chuck 5 outside the metal tube 4, chuck 5 two ends and metal tube outer wall link together, perhaps will seal up between chuck two ends and the metal tube by blockage structure, between metal tube 4 and chuck 5, constitute the chamber of sealing, form evaporation tube and be used to feed cold-producing medium, be connected with refrigerant inlet pipe 3 on the chuck 5 and refrigerant outlet pipe 8 communicates with chamber, be used for being connected in the evaporation tube of formation between metal tube 4 and chuck 5 and feed refrigerated medium with peripheral refrigeration plant.The front end of metal tube 5 forms soft tissue clamping hole 1 and is used to insert soft tissue.For ease of soft tissue is introduced in the metal tube 5 easily with filament, line etc., metal tube 4 other ends can be opened to form bullport 14.
During use soft tissue is introduced in the soft tissue clamping hole 1 of metal tube 4 one ends, feed cold-producing medium by refrigerant inlet pipe 3, and make chamber between full chuck 5 of cold-producing medium and the metal tube 4, utilize the liquid refrigerant vaporization of in this chamber, reducing pressure to take away the heat of evaporation tube rapidly and make it temperature and reduce, and be recovered into along refrigerant outlet pipe 8 in the compressor of refrigeration plant and carry out circulating next time.Utilize this process of refrigerastion to make the temperature of metal tube 4 be reduced to subzero, for example be reduced to-10 ℃, minimum reaching-20 ℃ (degree that temperature reduces depends on the volume size of evaporation tube and the performance of cold-producing medium).Because the inwall as the metal tube 4 of soft tissue grip tube is not to be an absolute light sliding surface, so will inlay involutory with the copper pipe inwall with soft thing after moisture soft thing contacts when it.When the temperature of metal tube 4 be down to zero following after, the adhesive surface of soft tissue and metal tube 4 inwalls can be frozen into a hard entity, the soft tissue grip tube that metal tube 4 constitutes bondingly realizes grasping the soft tissue piece by what low temperature formed down.This method can be delivered to whole test specimen uniformly with stress, and the stress that does not have clamp pressure to cause is concentrated, and also can guarantee the excellent of test specimen after thawing, and is a kind of desirable clamp method.
Be outside equipped with a waterproof and heat-insulating overcoat 7 at chuck 5, chuck 5 is enclosed in this waterproof and heat-insulating overcoat 7, and metal tube 4 one ends are positioned at waterproof and heat-insulating overcoat 7 and have through hole relative with the mouth of pipe of the soft tissue clamping hole 1 of metal tube 4 these ends in waterproof and heat-insulating overcoat end.For anti-sealing enters in the space between waterproof and heat-insulating overcoat 7 and the evaporation tube, be with an O-ring seal 2 in metal tube 4 ends, so that between the two gap is sealed.For easy to process, insulated sheath 7 is a tubular, and its opening one end seals by the outer upper shield 9 of waterproof and heat-insulating.Metal tube 4 other ends and refrigerant inlet pipe 3 and refrigerant outlet pipe 8 are piercing in outside the outer upper shield 9 of this waterproof and heat-insulating.Between waterproof and heat-insulating overcoat 7 and chuck 5, be filled with insulation material 6 or form vacuum, constitute the insulation layer, it can be applied in the water-bath environment.
Mechanics sensor can be easily links together with the metal tube of this chuck, is used to test the variation of pulling force and displacement.For ease of fast be connected easily, as depicted in figs. 1 and 2, be piercing in a connection of the welding of the end outside the upper shield 9 bridgeware 10 outside the waterproof and heat-insulating at metal tube 4, connecting bridgeware 10 can atresia, mesopore also can be set as depicted in figs. 1 and 2 pass to mutually with metal tube 4 and facilitate the introduction of soft tissue, connect bridgeware 10 other ends and be provided with internal thread hole.One by the good heat-barrier material double-threaded screw 11 made of nylon or other resin materials for example, an one thread end 12 is connected internal thread hole one end that connects bridgeware 10 by external thread, another thread end 13 is exposed as shown in Figure 2, can be used for connecting easily mechanics sensor, this moment, this chuck can be used as the upper grip use.Dexterously the double-threaded screw 11 that metal tube 4 and heat-barrier material are made is coupled together by connecting bridgeware 10, and utilize heat insulation between other equipment such as metal tube that heat insulation double-threaded screw realizes and pulling force sensor.
Fig. 1 uses as lower chuck, for the ease of carrying out fixing easily with the mechanical test machine platform, a lower chuck base 16 can also be set, lower chuck base 16 have one go up disk by many screw retention outside waterproof and heat-insulating on the upper shield 9, or directly be fixed on the waterproof and heat-insulating overcoat 7.The thread end 13 of double-threaded screw 11 is to be connected on lower chuck base 16 and the lower chuck base lower disc 19, be provided with on the lower chuck base lower disc 19 of lower chuck base 16 ends and pass the through hole 20 that set bolt is used, and can be fixed in easily on the mechanical test machine platform by lower chuck base lower disc 19, guaranteed freezing grip tube axial stability.Double-threaded screw 11 can be provided with axle center hole equally to facilitate the introduction of soft tissue, axle center hole can certainly be set otherwise with in the soft tissue clamping hole.The thread end 12 of double-threaded screw 11 is anti-tooth, and thread end 13 is positive tooth, so that be connected under the situation of not rotating chuck, sensor and base.
As shown in Figure 1, the through hole relative with double-threaded screw 11 can also be set as required to introduce soft tissue in soft tissue clamping hole 1 with instrument.Lower chuck base 16 is sleeved on and connects outside bridgeware 10 and the double-threaded screw, and at lower chuck base 16 hole is set and is used for refrigerant inlet pipe 3 and refrigerant outlet pipe 8 are drawn.
Though metal generally all has good thermal conductivity, can be used as metal tube 4, metal tube 4 of the present utility model adopts copper tube, and its external diameter is φ 4mm, the interior φ 3mm of being.It is φ 20mm copper tube that chuck 5 adopts external diameter.The evaporation tube that this employing red copper constitutes, heat-transfer effect is good, and the internal diameter of its metal tube 4 can satisfy the requirement of most of test to the soft tissue clamping.Waterproof and heat-insulating overcoat end is that φ 3mm is consistent with metal tube 4 internal diameters accordingly.
Refrigerant inlet pipe and refrigerant outlet pipe are connected to the two ends of chuck, and this mode is with respect to other connected modes, can guarantee that cold-producing medium flows through and fill whole chamber.
Upper grip refrigerating plant 20 and lower chuck refrigerating plant 28 are refrigeration machines, are prior art.
Ebullator 23, temperature sensor 24 and heat-generating pipe 25 are housed in the thermostatted water bathtub 22, and heat-generating pipe 25 and temperature sensor 24 are connected with input port with the output of a temperature controller 26 respectively.
This 37 ℃ brine bath of the present utility model is bathed the freezing anchor clamps of biological soft tissue stretching test, and simple in structure, processing and manufacturing is also more convenient, thereby cost is lower.Can also be used for 37 ℃ of water-bath environment in addition, can experimentize, improve experimental level, increase the meaning of scientific research at the simulation physiological condition.
With reference to shown in Figure 3, the using method of brine bath biological soft tissue stretching test anchor clamps is as follows:
1. chuck is installed: the refrigerating plant 20 of upper grip 21 is fixed on the lifting table of mechanical test machine 35, and the refrigerating plant 28 of lower chuck 27 is fixed on the base of mechanical test machine.Cleansing bath tub 22 is fixed in lower chuck 27 upper ends, is connected with mechanics sensor on the upper grip.
2. connecting that loading is made as the soft tissue cross-sectional area at normal temperatures can just be by the size of organizing clamping hole 1 (φ 3mm) of upper grip and lower chuck, can improve the aperture of chuck copper pipe according to the sample size of required test, with finer wire simple fixation soft tissue one end, in soft tissue clamping hole 1 is pulled metal tube 4 as freezing grip tube into, adjust the degree of depth of pulling into according to actual needs.
3. after the two ends that starting freezes treats sample are all pulled freezing clamp 21, lower chuck 27 respectively into, start the refrigerating plant 20,28 of two chucks simultaneously.Temperature in the freezing grip tube can be reduced to subzero 10 ℃ in 3-5 minute, even lower.This moment, soft tissue condensed as a wholely with freezing grip tube, just can carry out next step water filling.
4. the water filling heating is because freezing grip tube far-end is laid waterproof apron 2, add again as tissue and tube wall good bond in the metal tube 4 of freezing grip tube, hot water in the experimental situation is can not enter into to organize in the clamping hole, guaranteed that loading procedure can carry out in hot bath, this also is the most ingenious and distinctive feature of this invention.In cleansing bath tub 22, inject normal temperature physiological saline, connect ebullator 23, temperature controller 26 and heat-generating pipe 25 power supplys.The effect of ebullator 23 is to make water body temperature homogeneous, and temperature controller 26 can show real-time water temperature and water temperature is controlled at 37 ± 1 ℃.
5. mechanics loads that water temperature reaches 37 ℃ after 5-10 minute, and the temperature in the freezing grip tube 4 are reduced to subzero 5 ℃-10 ℃, just can start the mechanics test machine and carry out the mechanics loading experiment.

Claims (10)

1, a kind of brine bath biological soft tissue stretching test anchor clamps, comprise the lower chuck and the upper grip that are installed on the mechanical test machine, it is characterized in that: also comprise a thermostatted water bathtub, lower chuck from described thermostatted water bath base stretch into and and the water bath with thermostatic control cylinder bottom between form sealing, described upper grip self-constant temperature cleansing bath tub top is stretched in the described thermostatted water bathtub and is relative with lower chuck; Described upper grip and lower chuck include a metal tube, metal tube is with chuck outward, the chamber that constitutes sealing between metal tube and the chuck forms evaporation tube and is used to feed cold-producing medium, being connected with refrigerant inlet pipe and refrigerant outlet pipe on the chuck communicates with described chamber, described chuck is encapsulated in the waterproof and heat-insulating overcoat, described metal tube one end is positioned at the waterproof and heat-insulating overcoat, there is through hole relative in waterproof and heat-insulating overcoat end with the mouth of pipe of this end of metal tube, and this end has O-ring seal between metal tube and waterproof and heat-insulating overcoat, the described metal tube other end and refrigerant inlet pipe and refrigerant outlet pipe are piercing in outside this insulated sheath, be filled with insulation material or form vacuum between described waterproof and heat-insulating overcoat and described chuck.
2, brine bath biological soft tissue stretching test anchor clamps as claimed in claim 1, it is characterized in that: described waterproof and heat-insulating overcoat is a tubular, its opening one end is airtight with this openend with the outer upper shield of waterproof and heat-insulating, and described metal tube and refrigeration squeeze inlet tube and refrigerant outlet pipe upper shield outside this waterproof and heat-insulating passes.
3, brine bath biological soft tissue stretching test anchor clamps as claimed in claim 1 or 2, it is characterized in that: one of the end welding that is piercing in outside the waterproof and heat-insulating overcoat at described metal tube connects bridgeware, connect the bridgeware atresia or mesopore is set and communicate with metal tube, connect the bridgeware other end and be provided with internal thread hole, double-threaded screw one end of being made by heat-barrier material is assemblied in the internal thread hole of described connection bridgeware by external thread structure.
4, brine bath biological soft tissue stretching test anchor clamps as claimed in claim 3, it is characterized in that: described double-threaded screw is provided with axle center hole.
5, brine bath biological soft tissue stretching test anchor clamps as claimed in claim 3 is characterized in that: described double-threaded screw is anti-tooth with a described end that is connected the bridgeware connection, and the other end is positive tooth.
6, brine bath biological soft tissue stretching test anchor clamps as claimed in claim 3, it is characterized in that: described lower chuck also is provided with a lower chuck base, the lower chuck base has a last disk to put outside waterproof and heat-insulating by many screw retention, be provided with on the lower disc of the lower chuck base other end and pass the through hole that set bolt is used, at the lower chuck base hole is set and is used for refrigerant inlet pipe and refrigerant outlet pipe are drawn.
7, brine bath biological soft tissue stretching test anchor clamps as claimed in claim 6 is characterized in that: a thread end of described double-threaded screw is connected on the described lower chuck base.
8, brine bath biological soft tissue stretching test anchor clamps as claimed in claim 7 is characterized in that: the lower disc of described lower chuck base has through hole axially relative with the mouth of pipe of described metal tube.
9, brine bath biological soft tissue stretching test anchor clamps as claimed in claim 1 or 2 is characterized in that: described metal tube adopts copper tube, and its external diameter is Φ 4mm, the interior Φ 3mm of being; It is Φ 20mm copper tube that described chuck adopts external diameter, and described refrigerant inlet pipe and refrigerant outlet pipe are connected to the two ends of described chuck.
10, brine bath biological soft tissue stretching test anchor clamps as claimed in claim 1 or 2, it is characterized in that: in the described thermostatted water bathtub ebullator, temperature sensor and heat-generating pipe are housed, described heat-generating pipe and temperature sensor are connected with input port with the output of a temperature controller respectively.
CN 200620175985 2006-12-27 2006-12-27 Salt bath biological soft tissue tensile test fixture Expired - Fee Related CN200993623Y (en)

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Application Number Priority Date Filing Date Title
CN 200620175985 CN200993623Y (en) 2006-12-27 2006-12-27 Salt bath biological soft tissue tensile test fixture

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102564846A (en) * 2010-12-22 2012-07-11 首都医科大学附属北京口腔医院 Constant temperature water bath circulation device simulating oral cavity environment and method for testing stress by utilizing same
CN103115816A (en) * 2011-11-17 2013-05-22 江苏亚星锚链股份有限公司 Slow strain rate test method and device for R6-stage mooring cable
CN106872272A (en) * 2017-02-23 2017-06-20 北京理工大学 A kind of dissection of aorta diaphragm organization mechanicses attribute determines devices and methods therefor
CN107966361A (en) * 2017-12-18 2018-04-27 宋越 Liquid nitrogen refrigerating type fixture and biological mechanics determining equipment
CN109163965A (en) * 2018-09-26 2019-01-08 姜军 A kind of chain strength detection device of chain-bucket dredge boat
CN110132723A (en) * 2019-04-23 2019-08-16 江苏科技大学 Organization mechanics performance testing device and test method under a kind of imitative biological living environment
CN112683667A (en) * 2020-12-02 2021-04-20 北京市理化分析测试中心 System and method for testing tensile property of soft hydrophilic material in physiological saline

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102564846A (en) * 2010-12-22 2012-07-11 首都医科大学附属北京口腔医院 Constant temperature water bath circulation device simulating oral cavity environment and method for testing stress by utilizing same
CN103115816A (en) * 2011-11-17 2013-05-22 江苏亚星锚链股份有限公司 Slow strain rate test method and device for R6-stage mooring cable
CN106872272A (en) * 2017-02-23 2017-06-20 北京理工大学 A kind of dissection of aorta diaphragm organization mechanicses attribute determines devices and methods therefor
CN107966361A (en) * 2017-12-18 2018-04-27 宋越 Liquid nitrogen refrigerating type fixture and biological mechanics determining equipment
CN109163965A (en) * 2018-09-26 2019-01-08 姜军 A kind of chain strength detection device of chain-bucket dredge boat
CN109163965B (en) * 2018-09-26 2020-12-15 江苏丰源船舶工程有限公司 Chain strength detection device of chain bucket type sand dredger
CN110132723A (en) * 2019-04-23 2019-08-16 江苏科技大学 Organization mechanics performance testing device and test method under a kind of imitative biological living environment
CN110132723B (en) * 2019-04-23 2021-07-27 江苏科技大学 Device and method for testing tissue mechanical properties in bionic living body environment
CN112683667A (en) * 2020-12-02 2021-04-20 北京市理化分析测试中心 System and method for testing tensile property of soft hydrophilic material in physiological saline

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Granted publication date: 20071219

Termination date: 20101227