CN1995365A - Method for preparing high concentration gamma-aminobutyric acid powder using rice bran as material - Google Patents
Method for preparing high concentration gamma-aminobutyric acid powder using rice bran as material Download PDFInfo
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- CN1995365A CN1995365A CN 200610166426 CN200610166426A CN1995365A CN 1995365 A CN1995365 A CN 1995365A CN 200610166426 CN200610166426 CN 200610166426 CN 200610166426 A CN200610166426 A CN 200610166426A CN 1995365 A CN1995365 A CN 1995365A
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- jidingsuan
- rice bran
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Abstract
The invention discloses a making method of high-density gamma-aminobutyric acid powder, which comprises the following steps: adopting rice bran as raw material; adding glutamic acid, calcium chloride and phosphopyridoxal to obtain the reacting liquid with rich gamma-aminobutyric acid through endogenous enzyme; centrifuging and separating to obtain supernatant; adding alcohol to remove impurity; hyperfiltering; condensing; drying to obtain the product with content over 50%.
Description
Technical field
A kind of is the method for feedstock production high density γ-An Jidingsuan powder with the rice bran, belongs to rice intensive processing technical field.
Background technology
Ground in the brown rice pearling process down cortex be rice bran, it plants skin by pericarp, megarchidium layer, mixtures such as aleurone layer and Rice plumule and small brokens bits composition.In the course of processing of rice, at first by going rice husk and paddy shell to obtain brown rice, secondly pearling obtains rice by the brown cortex of removing the brown rice outside, main by product rice bran comprises aleurone layer, the megarchidium layer, pericarp layer and the several subgrades between them, but the rice bran in the actual production also is mixed with rice germ and cracks rice.The composition of full-cream rice bran has the protein of 12%-18%, the fat of 16%-20%, and about 12% ash content and about 50% carbohydrate, wherein carbohydrate comprises starch, phytate, hemicellulose, Mierocrystalline cellulose and piperylene, utility value is very high.Paddy is the staple food crop of China, and annual production can reach more than 1.8 hundred million tons, and rice bran accounts for its 5-8% again, is a kind of superior renewable resources.
At present, many successful experiences on the comprehensive development and utilization of rice bran, have been obtained abroad.The U.S. researchs and develops rice bran resource tool authority's one of country the earliest in the world.It is reported, U.S. paddy Creative Company and Li Pu Man in extractions such as rice bran stabilization technology and rice bran nutrients, rice bran nutrient fibre, rice bran protein, rice bran polysaccharides, separate, existing important breakthrough aspect the purification technique, multinomial technology can be promoted and be used.Studies have shown that rice bran can rise in value about 60 times through the deep development utilization.
γ-An Jidingsuan (GABA) is the inhibitory nerve transmitter substance that is present in mammal brain, the spinal cord, by L-glutamic acid through L-Glutamic decarboxylase (GAD) catalyzed conversion.Studies show that that GABA has is hypotensive, anti-ageing, improve the brain function, ataraxy, promotion long-term memory, promote effects such as growth hormone secretion, renal function activation, liver function activation, is applied to will be well worth doing in the functional foodstuff.In recent years, be rich in the research and development of GABA food, comprise the GABA food of plant (Rice plumule, rice bran, green tea, pumpkin etc.), microorganism origin such as (milk-acid bacteria, yeast), become abroad, the focus of Japanology especially.
Because the GAD in the rice mainly concentrates on the plumule position, sneaks into rice bran together with cortex during husk rice.Therefore utilize rice bran to come enrichment GABA, preparation GABA foodstuff additive are the most economical suitable.Though the exploitation of the sprout brown rice that is rich in GABA is also arranged in the rice goods, the GABA enriching quantity of sprout brown rice is very limited, does not add Glu, and the highest enriching quantity of GABA can only reach 40mg/100g; And the content of GABA just has 47mg/100g in the rice bran before enrichment, as long as simple water logging bubble technology just can improve octuple.And sprout brown rice is edible very inconvenient, and mouthfeel is also poor, so application prospect is not as rice bran.Coming enrichment GABA with rice bran, is the GABA product method of economical rationality comparatively of preparation high density.
Summary of the invention
The purpose of this invention is to provide a kind of is the method for feedstock production high density γ-An Jidingsuan powder with the rice bran, and the γ-An Jidingsuan powder heath food with nourishing function that can the production high added value is given full play to the trophic function and the effect of rice bran.
Technical scheme of the present invention: with the rice bran is raw material, add L-glutamic acid (Glu), calcium chloride and pyridoxal phosphate (PLP), obtain being rich in the reaction solution of γ-An Jidingsuan (GABA) by the endogenous enzyme reaction, centrifugation obtains supernatant liquor, add ethanol in the gained supernatant liquor and remove polysaccharide impurity, carry out ultrafiltration again, concentrate and drying after obtain high density γ-An Jidingsuan powder.
(1) preparation γ-An Jidingsuan pregnant solution: rice bran and water are in bulking value solid-liquid ratio 1: 6-1: 8, add the Glu of the 0.1%-0.5% of rice bran weight, the CaCl of 10-15mmol/mol Glu
2, the PLP of 0.5-1mmol/L, 40 ℃ of temperature, pH5.6, reaction times 6-8 hour.
(2) purifying of γ-An Jidingsuan: supernatant liquor is got in above-mentioned pregnant solution centrifugation, added supernatant liquor volume 70%-80% volume of ethanol, precipitate 12 hours, supernatant liquor is got in centrifugation.Adopt the inorganic ceramic film of 50 nanometers under 0.2MPa pressure, to carry out ultrafiltration.
(3) preparation high density γ-An Jidingsuan powder: ultrafiltration is seen through liquid concentrate with falling film type economic benefits and social benefits thickener, thickening temperature is 60-65 ℃, is concentrated into that solid content reaches 20%-25% in the feed liquid.Feed liquid after concentrating is then carried out spraying drying, and air outlet temperature is 85 ℃, and moisture content is below 5%, and dried product is high density γ-An Jidingsuan powder.
The application of high density γ-An Jidingsuan powder can be used for blood pressure regulation, the anti-ageing health care foods of waiting for a long time.
Beneficial effect of the present invention, in sum, rice bran can be used as a kind of nutritious, heath food raw material that physiological function is remarkable and further development and use, so not only can improve the economic benefit of rice processing comprehensive utilization value and grain-production, also can make grain-production go on eucyclic road.With the rice bran is the research and development of feedstock production high density γ-An Jidingsuan powder, belongs to initiative work at home.The present invention uses modern biotechnology and separating and purifying technology that the preparation technology of high density γ-An Jidingsuan powder is provided, and the high density γ-An Jidingsuan powder for preparing can be used for blood pressure regulation, the anti-ageing health care foods of waiting for a long time.
Embodiment
Embodiment 1
Take by weighing the rice bran of 15kg, add the water of 90kg, add 15g Glu, 55mg Cacl
2, oscillatory reaction is 8 hours under pH5.6 and 40 ℃ of temperature.Supernatant liquor is got in centrifugation, adds the volume ratio of ethanol to 70%, precipitates 12 hours, and supernatant liquor is got in centrifugation.Adopt the inorganic ceramic film of 50 nanometers under 0.2MPa pressure, to carry out ultrafiltration.Ultrafiltration is seen through liquid with falling film type economic benefits and social benefits thickener, concentrate seeing through liquid under 60 ℃ condition, the feed liquid after concentrating is carried out spraying drying, and air outlet temperature is 85 ℃, and dried product is high density γ-An Jidingsuan powder.
Embodiment 2
Take by weighing the rice bran of 15kg, add the water of 120kg, add 75g Glu, 65mgCacl
2, oscillatory reaction is 6 hours under pH5.6 and 40 ℃ of temperature.Supernatant liquor is got in centrifugation, adds the volume ratio of ethanol to 80%, precipitates 12 hours, and supernatant liquor is got in centrifugation.Adopt the inorganic ceramic film of 50 nanometers under 0.2MPa pressure, to carry out ultrafiltration.Ultrafiltration is seen through liquid with falling film type economic benefits and social benefits thickener, concentrate seeing through liquid under 65 ℃ condition, the feed liquid after concentrating is carried out spraying drying, and air outlet temperature is 85 ℃, and dried product is high density γ-An Jidingsuan powder.
Claims (1)
1, a kind of is the method for feedstock production high density γ-An Jidingsuan powder with the rice bran, it is characterized in that with the rice bran being raw material, add L-glutamic acid, calcium chloride and pyridoxal phosphate, obtain being rich in the reaction solution of γ-An Jidingsuan by the endogenous enzyme reaction, centrifugation obtains supernatant liquor, add ethanol in the gained supernatant liquor and remove polysaccharide impurity, carry out ultrafiltration again, concentrate and drying after obtain high density γ-An Jidingsuan powder:
(1) preparation γ-An Jidingsuan pregnant solution: rice bran and water are in bulking value solid-liquid ratio 1: 6-1: 8, add the L-glutamic acid of the 0.1%-0.5% of rice bran weight, the CaCl of 10-15mmol/mol L-glutamic acid
2, the pyridoxal phosphate of 0.5-1mmol/L, 40 ℃ of temperature, pH5.6, reaction 6-8 hour;
(2) purifying of γ-An Jidingsuan: supernatant liquor is got in the centrifugation of γ-An Jidingsuan pregnant solution, added supernatant liquor volume 70%-80% volume of ethanol, precipitate 12 hours, supernatant liquor is got in centrifugation; Adopt the inorganic ceramic film of 50 nanometers under 0.2MPa pressure, to carry out ultrafiltration;
(3) preparation high density γ-An Jidingsuan powder: ultrafiltration is seen through liquid concentrate with falling film type economic benefits and social benefits thickener, thickening temperature is 60-65 ℃, be concentrated into that solid content reaches 20%-25% in the feed liquid, feed liquid after concentrating is then carried out spraying drying, air outlet temperature is 85 ℃, moisture content is below 5%, and dried product is high density γ-An Jidingsuan powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200610166426XA CN100478447C (en) | 2006-12-15 | 2006-12-15 | Method for preparing high concentration gamma-aminobutyric acid powder using rice bran as material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200610166426XA CN100478447C (en) | 2006-12-15 | 2006-12-15 | Method for preparing high concentration gamma-aminobutyric acid powder using rice bran as material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1995365A true CN1995365A (en) | 2007-07-11 |
| CN100478447C CN100478447C (en) | 2009-04-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB200610166426XA Expired - Fee Related CN100478447C (en) | 2006-12-15 | 2006-12-15 | Method for preparing high concentration gamma-aminobutyric acid powder using rice bran as material |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101273769B (en) * | 2008-04-24 | 2011-08-17 | 江南大学 | Method for preparing germinated brown rice with high-content gamma-aminobutyric acid |
| CN101810322B (en) * | 2009-02-20 | 2012-11-14 | 南京工业大学 | Method for producing food rich in gamma-aminobutyric acid |
| CN102793171A (en) * | 2012-08-29 | 2012-11-28 | 陕西科技大学 | Method of increasing gamma-aminobutyric acid in buckwheat tea |
| CN103436515A (en) * | 2013-08-29 | 2013-12-11 | 江苏丘陵地区镇江农业科学研究所 | Gamma-aminobutyric acid (GABA)-enriched rapeseed powder and method for producing GABA-enriched rapeseed powder employing watermelon peel juice |
| CN108640848A (en) * | 2018-05-21 | 2018-10-12 | 佛山实瑞先导材料研究院（普通合伙） | The extracting method of γ-aminobutyric acid in a kind of seawater rice plumule |
-
2006
- 2006-12-15 CN CNB200610166426XA patent/CN100478447C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101273769B (en) * | 2008-04-24 | 2011-08-17 | 江南大学 | Method for preparing germinated brown rice with high-content gamma-aminobutyric acid |
| CN101810322B (en) * | 2009-02-20 | 2012-11-14 | 南京工业大学 | Method for producing food rich in gamma-aminobutyric acid |
| CN102793171A (en) * | 2012-08-29 | 2012-11-28 | 陕西科技大学 | Method of increasing gamma-aminobutyric acid in buckwheat tea |
| CN103436515A (en) * | 2013-08-29 | 2013-12-11 | 江苏丘陵地区镇江农业科学研究所 | Gamma-aminobutyric acid (GABA)-enriched rapeseed powder and method for producing GABA-enriched rapeseed powder employing watermelon peel juice |
| CN108640848A (en) * | 2018-05-21 | 2018-10-12 | 佛山实瑞先导材料研究院（普通合伙） | The extracting method of γ-aminobutyric acid in a kind of seawater rice plumule |
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| Publication number | Publication date |
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| CN100478447C (en) | 2009-04-15 |
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| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Jiangsu Danlv Rice Co.,Ltd. Assignor: Jiangnan University Contract record no.: 2011320000526 Denomination of invention: Method for preparing high concentration gamma-aminobutyric acid powder using rice bran as material Granted publication date: 20090415 License type: Exclusive License Open date: 20070711 Record date: 20110406 |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090415 Termination date: 20171215 |
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| CF01 | Termination of patent right due to non-payment of annual fee |