CN1995070A - Ultrasonic wave extraction process for rice bran polysaccharide - Google Patents
Ultrasonic wave extraction process for rice bran polysaccharide Download PDFInfo
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- CN1995070A CN1995070A CN 200610166326 CN200610166326A CN1995070A CN 1995070 A CN1995070 A CN 1995070A CN 200610166326 CN200610166326 CN 200610166326 CN 200610166326 A CN200610166326 A CN 200610166326A CN 1995070 A CN1995070 A CN 1995070A
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Abstract
The invention discloses an ultrasonic extracting method of rice bran polysaccharide in the rice deep-manufacturing technical domain, which comprises the following steps: grinding rice bran; extracting through ultrasound; centrifuging; filtering to obtain the filtrate; condensing to remove protein through trichloroacetic acid method; centrifuging; adding alcohol in the supernatant to stir completely; stewing; collecting certain density scale of alcohol sediment; freezing; drying to obtain the product.
Description
Technical field
A kind of supersonic extracting method of rice bran polysaccharide the present invention relates to a kind of extracting method of polysaccharide, and particularly the extracting method of rice bran polysaccharide belongs to paddy deep process technology field.
Background technology
Rice bran is a byproduct of rice processing, about 9,000,000 tons of China's annual production, and real is a kind of renewable resources that has a large capacity and a wide range.The United Nations Industrial Development Organization calls a kind of raw material of underusing to rice bran, and the further development and utilization of rice bran can be foodstuffs industry abundant raw materials for production are provided.
" rice bran, it is sweet flat, nontoxic to distinguish the flavor of, and has the function of logical intestines, appetizing, the therapeutic method to keep the adverse qi flowing downward, the long-pending piece of mill " on the books on China's ancient book.The active substance that contains function brilliances such as abundant active polysaccharide, protein, fat, tocopherol in the rice bran.Rice bran polysaccharide has significant biological activity and physiological hygiene function, has multiple functions such as antitumor, hypoglycemic, decreasing cholesterol, bacterial-infection resisting and strengthening immunity.
Since the eighties in 20th century, fast development along with glycobiology, being subjected to people about the research of the structure of rice bran polysaccharide and physiologically active aspect and more and more having paid attention to, is one of primary problem that solves and seek a kind of extracting method easy, quick, that efficiently and not destroy its physiologically active ingredient.
Summary of the invention
The supersonic extracting method that the purpose of this invention is to provide a kind of rice bran polysaccharide is a kind of science, extracts rice bran polysaccharide efficiently, and can keep the rice bran polysaccharide biological activity, improves the method for rice bran polysaccharide extraction yield to greatest extent.
Technical scheme of the present invention: a kind of supersonic extracting method of rice bran polysaccharide in turn includes the following steps: 1. be that raw material is pulverized it with the rice bran; 2. the rice bran of getting a certain amount of pulverizing adds the water of 10-15 times of weight, with the ultrasonic extraction method rice bran polysaccharide, gets extracting solution after the centrifuging; 3. extracting solution is placed rotatory evaporator to be evaporated to 1/10 of former extracting liquid volume in 60 ℃, centrifugal collection supernatant liquor removes albumen with trichloroacetic acid method, gets supernatant liquor; 4. add ethanol and fully stir, leave standstill in supernatant liquor, collect the ethanol sedimentation of finite concentration scope, lyophilize promptly gets the rice bran polysaccharide extract.
Pulverising step is for to be broken to bran powder below 150 orders.The ultrasonic extraction step is ultrasonic wave extraction time 10-30 minute, and ultrasonic power 200-400W gets extracting solution after the centrifuging.Remove proteic step for add trichoroacetic acid(TCA) reagent in extracting solution, make the final concentration of trichoroacetic acid(TCA) reach 8%-15%, centrifugal removal albumen precipitation repeats the proteic operation of above-mentioned removal, carries out 2-5 time.The alcohol precipitation step left standstill 24-72 hour at 0-8 ℃ for adding the ethanol of 95% concentration, and collecting ethanol final concentration scope is the precipitation of 60%-90%, and lyophilize promptly gets the rice bran polysaccharide extract.
Beneficial effect of the present invention: the present invention adopts pulverizing, ultrasonic extraction, Deproteinization, alcohol precipitation and lyophilize extracting method to obtain the rice bran polysaccharide extract, the craft science that is proposed, rationally, have an advantage quick, easy, that cost is low, yield is high, not only can save energy but also kept many active substances in the rice bran polysaccharide, improved the yield of rice bran polysaccharide to greatest extent, reach 2.87%, and help further studying rice bran polysaccharide activity and structure activity relationship.
Description of drawings
The ultrasonic extracting process schema of Fig. 1 rice bran polysaccharide.
Embodiment
Embodiment 1: step is extracted rice bran polysaccharide by the following method.
A, pulverising step: bran powder is broken to below 100 orders.
B, ultrasonic extraction step: get 2 kilograms of rice bran crushed materials and add 20 kilograms in water, at ultrasonic power 250W, the supersound extraction time is 15 minutes, gets supernatant liquor after the centrifuging.
C. remove the albumen step: in ultrasonic extraction gained supernatant liquor, add trichoroacetic acid(TCA), make final concentration reach 10%, repetitive operation, centrifugal removal albumen 5 times.
D. alcohol precipitation step: remove in the supernatant liquor behind the albumen and add 95% ethanol, left standstill 24 hours at 4 ℃, collecting the alcohol concn scope is the precipitation that 60%-90% separates out.
E. lyophilize step: the precipitation lyophilize of alcohol precipitation gained promptly gets the rice bran polysaccharide extract.
Embodiment 2: step is extracted rice bran polysaccharide by the following method.
A, pulverising step: bran powder is broken to below 80 orders.
B, ultrasonic extraction step: get 3 kilograms of rice bran crushed materials and add 40 kilograms in water, at ultrasonic power 350W, the supersound extraction time is 25 minutes, gets supernatant liquor after the centrifuging.
C. remove the albumen step: in ultrasonic extraction gained supernatant liquor, add trichoroacetic acid(TCA), make final concentration reach 12%, repetitive operation, centrifugal removal albumen 3 times.
D. alcohol precipitation step: remove in the supernatant liquor behind the albumen and add 95% ethanol, left standstill 48 hours at 6 ℃, collecting the alcohol concn scope is the precipitation that 60%-90% separates out.
E. lyophilize step: the precipitation lyophilize of alcohol precipitation gained promptly gets the rice bran polysaccharide extract.
Claims (5)
1, a kind of supersonic extracting method of rice bran polysaccharide is characterized in that in turn including the following steps: 1. be that raw material is pulverized it with the rice bran; 2. the rice bran of getting a certain amount of pulverizing adds the water of 10-15 times of weight, with the ultrasonic extraction method rice bran polysaccharide, gets extracting solution after the centrifuging; 3. extracting solution is placed rotatory evaporator to be evaporated to 1/10 of former extracting liquid volume in 60 ℃, centrifugal collection supernatant liquor removes albumen with trichloroacetic acid method, gets supernatant liquor; 4. add ethanol and fully stir, leave standstill in supernatant liquor, collect the ethanol sedimentation of finite concentration scope, lyophilize promptly gets the rice bran polysaccharide extract.
2, the supersonic extracting method of rice bran polysaccharide according to claim 1 is characterized in that described pulverising step is for to be broken to bran powder below 150 orders.
3, the supersonic extracting method of rice bran polysaccharide according to claim 1 is characterized in that described ultrasonic extraction step is ultrasonic wave extraction time 10-30 minute, and ultrasonic power 200-400W gets extracting solution after the centrifuging.
4, the supersonic extracting method of rice bran polysaccharide according to claim 1, it is characterized in that the proteic step of described removal is for adding trichoroacetic acid(TCA) reagent in extracting solution, make the final concentration of trichoroacetic acid(TCA) reach 8%-15%, centrifugal removal albumen precipitation, repeat the proteic operation of above-mentioned removal, carry out 2-5 time.
5, the supersonic extracting method of rice bran polysaccharide according to claim 1, it is characterized in that described alcohol precipitation step for adding the ethanol of 95% concentration, left standstill 24-72 hour at 0-8 ℃, collecting ethanol final concentration scope is the precipitation of 60%-90%, lyophilize promptly gets the rice bran polysaccharide extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101663267A CN100486998C (en) | 2006-12-26 | 2006-12-26 | Ultrasonic wave extraction process for rice bran polysaccharide |
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| CNB2006101663267A CN100486998C (en) | 2006-12-26 | 2006-12-26 | Ultrasonic wave extraction process for rice bran polysaccharide |
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| CN1995070A true CN1995070A (en) | 2007-07-11 |
| CN100486998C CN100486998C (en) | 2009-05-13 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101863999A (en) * | 2010-07-22 | 2010-10-20 | 天津商业大学 | Preparation method of pholiota nameko polysaccharide extract |
| CN103694368A (en) * | 2013-12-18 | 2014-04-02 | 河北科星药业有限公司 | Method for extracting rice bran polysaccharides |
| CN104306391A (en) * | 2014-09-18 | 2015-01-28 | 河北科星药业有限公司 | Rice bran polysaccharide oral liquid, injection and soluble powder and corresponding preparation method |
| CN106892998A (en) * | 2017-04-11 | 2017-06-27 | 四川省农业科学院农产品加工研究所 | A kind of brown rice polysaccharide and its preparation method and application |
| CN107259308A (en) * | 2017-06-23 | 2017-10-20 | 贺州学院 | A kind of plant source fresh-cut water chestnut yellow inhibitor and its methods for making and using same |
| CN107557408A (en) * | 2017-10-31 | 2018-01-09 | 无锡甜丰食品有限公司 | With the method for production oligoisomaltose of cracking rice |
| CN108325996A (en) * | 2017-12-23 | 2018-07-27 | 佛山科学技术学院 | A kind of method that abandoned biomass multi-direction synthetic utilizes |
| CN109879979A (en) * | 2019-03-06 | 2019-06-14 | 武汉轻工大学 | A kind of antioxidant and preparation method thereof |
| CN111419824A (en) * | 2020-04-09 | 2020-07-17 | 武汉轻工大学 | preparation method of pH responsive carrier, drug delivery system and preparation method thereof |
-
2006
- 2006-12-26 CN CNB2006101663267A patent/CN100486998C/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101863999A (en) * | 2010-07-22 | 2010-10-20 | 天津商业大学 | Preparation method of pholiota nameko polysaccharide extract |
| CN101863999B (en) * | 2010-07-22 | 2012-05-30 | 天津商业大学 | Preparation method of pholiota nameko polysaccharide extract |
| CN103694368A (en) * | 2013-12-18 | 2014-04-02 | 河北科星药业有限公司 | Method for extracting rice bran polysaccharides |
| CN103694368B (en) * | 2013-12-18 | 2015-10-28 | 河北科星药业有限公司 | The extracting method of rice bran polysaccharide |
| CN104306391A (en) * | 2014-09-18 | 2015-01-28 | 河北科星药业有限公司 | Rice bran polysaccharide oral liquid, injection and soluble powder and corresponding preparation method |
| CN106892998A (en) * | 2017-04-11 | 2017-06-27 | 四川省农业科学院农产品加工研究所 | A kind of brown rice polysaccharide and its preparation method and application |
| CN107259308A (en) * | 2017-06-23 | 2017-10-20 | 贺州学院 | A kind of plant source fresh-cut water chestnut yellow inhibitor and its methods for making and using same |
| CN107259308B (en) * | 2017-06-23 | 2020-07-03 | 贺州学院 | Plant source fresh-cut water chestnut yellowing inhibitor and preparation and application methods thereof |
| CN107557408A (en) * | 2017-10-31 | 2018-01-09 | 无锡甜丰食品有限公司 | With the method for production oligoisomaltose of cracking rice |
| CN108325996A (en) * | 2017-12-23 | 2018-07-27 | 佛山科学技术学院 | A kind of method that abandoned biomass multi-direction synthetic utilizes |
| CN109879979A (en) * | 2019-03-06 | 2019-06-14 | 武汉轻工大学 | A kind of antioxidant and preparation method thereof |
| CN111419824A (en) * | 2020-04-09 | 2020-07-17 | 武汉轻工大学 | preparation method of pH responsive carrier, drug delivery system and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN100486998C (en) | 2009-05-13 |
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