CN1994351A - Yeradetajo extract, its preparation method and use - Google Patents
Yeradetajo extract, its preparation method and use Download PDFInfo
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- CN1994351A CN1994351A CN 200610201098 CN200610201098A CN1994351A CN 1994351 A CN1994351 A CN 1994351A CN 200610201098 CN200610201098 CN 200610201098 CN 200610201098 A CN200610201098 A CN 200610201098A CN 1994351 A CN1994351 A CN 1994351A
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- herba ecliptae
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Abstract
The invention relates to a method for producing black ink plant extractive, wherein the method comprises that: removing foreign materials from collected fresh black ink plant, washing, and breaking, drying at 20-30Deg. C via microwave, breaking. The microwave low-temperature drying method can confirm integrality of active component, with simple process, without organic, to protect the thermal-sensitive component. The invention can reduce cost.
Description
Technical field: the present invention relates to a kind of Herba Ecliptae extract and its production and use, belong to technical field of pharmaceuticals.
Background technology: Herba Ecliptae (Eclipta prostrate L.) is the dry aerial parts of feverfew sweet wine intestinal, all has throughout the country, has long medicinal history.Herba Ecliptae has nourishing the liver and kidney, and the effect of cooling blood for hemostasis is used for odontoseisis, early whitening of beard and hair, vertigo and tinnitus, soreness of the waist and knees, deficiency of YIN heat in blood, haematemesis, epistaxis, hematuria, dysentery, metrostaxis etc.
At present, the application of Herba Ecliptae only limits to take orally, and nearly all is to be used as medicine with dry product.And existing Herba Ecliptae extract all is to adopt water extraction or alcohol extracting method.The applicant is through discovering extensively and profoundly, by smashing into the bright product of Herba Ecliptae to pieces pasty state, through microwave low-temperature drying, the Herba Ecliptae extract that obtains after the pulverizing has significant anastalsis and analgesia, antiinflammatory, detumescent effect, and traumatic injury, traumatic hemorrhage disease are demonstrated good curative effect.
Summary of the invention:
The objective of the invention is to: a kind of Herba Ecliptae extract and its production and use is provided.The preparation technology of Herba Ecliptae extract of the present invention is simple, does not need to introduce any organic reagent, to such an extent as to and can avoid the destroyed curative effect of medication that influences of thermal sensitivity ingredient.
The present invention constitutes like this: the preparation method of Herba Ecliptae extract is: the Herba Ecliptae herb of fresh collection is removed impurity, clean, smash to pieces, 20-30 ℃ of microwave drying pulverized, and promptly gets Herba Ecliptae extract.
Preparation method by above-mentioned Herba Ecliptae extract is extracted the Herba Ecliptae extract that obtains.
Herba Ecliptae extract of the present invention is used to prepare the Chinese medicine preparation of treatment traumatic injury, traumatic hemorrhage.
Described Chinese medicine preparation is gel, spray or unguentum.
Specifically, described gel adds water by Herba Ecliptae extract 200g, glycerol 50ml, propylene glycol 50ml, carbomer 60g and gelatin 10g and makes.
Described spray adds an amount of propellant by Herba Ecliptae extract 50g, ethanol 100ml and Arlacel-85 2g and makes.
Described unguentum adds vaseline by Herba Ecliptae extract 100g, hydroxyphenyl Ethyl formate 0.3g and lanoline 50g and makes.
As required, also can in above-mentioned Chinese medicine preparation, add coloring agent, antiseptic, spice, correctives, sweeting agent or other material.
Herba Ecliptae extract of the present invention must adopt fresh medical material to be used as medicine, if be used as medicine with dry product then do not treat the curative effect of traumatic injury, traumatic hemorrhage aspect.In order to verify this conclusion and to guarantee effect of the present invention, the applicant has carried out relevant pharmacodynamic experiment to the bright product extract of Herba Ecliptae with Herba Ecliptae dry product extract, and is specific as follows:
Observation index: Ovum Gallus domesticus album causes rat pedal swelling experiment (anti-acute inflammation effect); White mice is turned round body experiment (analgesic activity); Herba Ecliptae extract is to the influence in mice bleeding time; Herba Ecliptae extract is to the influence of clotting time of mice.
The bright product extract of Herba Ecliptae that this pharmacodynamic experiment adopted is the extract that the preparation method according to Herba Ecliptae extract of the present invention prepares.The Herba Ecliptae dry product preparation method of extract that is adopted is: it is an amount of to get Herba Ecliptae medical material dry product, add 10 times of water gagings and decoct 2 times, each 2 hours, filter, merging filtrate, being evaporated to relative density is the clear paste of 1.25 (60 ℃ of surveys), adds ethanol and makes that to contain alcohol amount be 70%, leaves standstill 24 hours, get supernatant, reclaim ethanol, drying under reduced pressure promptly gets Herba Ecliptae dry product extract.
1. bright product extract of Herba Ecliptae and Herba Ecliptae dry product extract cause the contrast experiment that pedal swelling influences behind the rat to Ovum Gallus domesticus album:
Method and result: get 40 of Wistar rat, weigh, use the picric acid labelling, be divided into 4 groups, 10 every group.Rat right hind leg is fixed with hands, made a sign in its ankle with ball pen, the right hind with rat immerses in the sufficient capacity measuring device again, and immersion depth is the boundary with the sign place.The numerical value that reads from the measuring pipette place of this device is the normal volume of the right foot of animal.Survey twice continuously, ask its meansigma methods.Irritate the following medicine of stomach respectively for 4 treated animals: the solution that the bright product extract of Herba Ecliptae makes, solution, hydrocortisone, the normal saline that Herba Ecliptae dry product extract makes, capacity is 1ml/100g.Behind the 30min in every Mus right hind centre of the palm to ankle joint direction inserting needle, subcutaneous injection 10% egg clear liquid 0.1ml/ is only.Thereafter per volume of measuring the Mus right hind in 0.5 hour respectively, continuous 3 times.
Experimental result (table 1) shows that the bright product extract of Herba Ecliptae has good antiinflammatory action, and its antiinflammatory action is stronger than hydrocortisone; Herba Ecliptae dry product extract does not then have the antiinflammatory effect.
Table 1 pair Ovum Gallus domesticus album causes the influence of pedal swelling behind the rat
| Group | The animal number of elements | Drug dose | Cause scorching back paw swelling/ml | ||
| mg·kg -1 | 0.5h | 1.0h | 1.5h | ||
| The normal saline group | 10 | - | 0.53±0.11 | 0.48±0.10 | 0.44±0.12 |
| The hydrocortisone group | 10 | 5％ | 0.36±0.10** | 0.32±0.08** | 0.25±0.11** |
| Herba Ecliptae dry product group | 10 | 10％ | 0.49±0.13 | 0.51±0.15 | 0.48±0.16 |
| The bright product group of Herba Ecliptae | 10 | 10％ | 0.28±0.12** | 0.26±0.12** | 0.19±0.13** |
Annotate: * * represents and the poor heteropole of normal saline group remarkable (P＜0.01).
2. bright product extract of Herba Ecliptae and dry product extract are to the contrast test (writhing method) of white mice analgesic activity:
Method and result: 60 of Kunming kind white mice, body weight 18-22g, ♀ ♂ half and half cuts the hair of white mice abdominal part, is divided into 3 groups at random, 20 every group.The administration group gavages bright product extract 0.3g/10g of Herba Ecliptae and Herba Ecliptae dry product extract 0.3g/10g, and matched group gavages the equivalent normal saline.30min after the administration, each Mus lumbar injection 0.6% acetum 0.2ml/ are only.Occur the number of times and the time of writhing response behind observation and the record injection acetum in the 15-30min, the results are shown in Table 2.
The result shows: the white mice writhing response has the obvious suppression effect due to the bright product extract of the Herba Ecliptae Dichlorodiphenyl Acetate, and Herba Ecliptae dry product extract does not then have the analgesia inhibitory action.
The influence of white mice writhing response due to table 2 Dichlorodiphenyl Acetate (x ± s)
| Group | Number of animals (only) | Drug level (%) | Turn round the body number of times in the 15min |
| The normal saline group | 20 | -- | 46.03±7.43 |
| Herba Ecliptae dry product extract group | 20 | 5 | 45.52±5.31 |
| The bright product extract of Herba Ecliptae group | 20 | 5 | 19.07±8.66** |
3. bright product extract of Herba Ecliptae and dry product extract are tested the comparative that influences of clotting time of mice:
Method and result: 40 of Kunming kind white mice, be divided into 4 groups at random, every group 10, it is the bright product extract of normal control group (normal saline), positive controls (YUNNAN BAIYAO) 0.2g/kg, Herba Ecliptae group (1.0g crude drug/kg) and Herba Ecliptae dry product extract group (1.0g crude drug/kg), gastric infusion, the administration volume is all with 0.15ml/10g mice weighing machine, and each treated animal is by above-mentioned dosage successive administration 3d; After administration in the 3rd day 1 hour is that the capillary glass tube of 1mm inserts mice endocanthion ball rear vein beard and gets blood with internal diameter, and the blood post reaches 5cm to the capillary glass-tube.A bit of every the 30s capillary glass-tube that fractures, check to have or not the blood clotting silk to occur.Calculating is taken a blood sample to from the capillary glass-tube and the time of blood clotting silk occurred, is clotting time.With Herba Ecliptae extract group, positive controls and normal control group relatively, organize a t check, the results are shown in Table 3.
The result shows: be index with the clotting time, the bright product extract of Herba Ecliptae group, YUNNAN BAIYAO group and normal saline group relatively have significant difference, have significant anastalsis; Herba Ecliptae dry product extract group does not then have this effect.
The influence of table 3 pair clotting time of mice
| Group | Dosage (g/kg) | Clotting time (second) |
| Matched group | --- | 100.9±11.6 |
| The YUNNAN BAIYAO group | 0.2 | 45.3±13.2 |
| The bright product extract of Herba Ecliptae group | 1.0 | 47.9±12.8 |
| Herba Ecliptae dry product extract group | 1.0 | 98.7±12.3 |
Bright product extract of Herba Ecliptae and Herba Ecliptae dry product extract to mice hemorrhage influence comparative tests:
Method and result: 40 of Kunming kind white mice, be divided into 4 groups at random, every group 10, it is the bright product extract of normal control group (normal saline), positive controls (YUNNAN BAIYAO) 0.2g/kg, Herba Ecliptae group (1.0g crude drug/kg) and Herba Ecliptae dry product extract group (1.0g crude drug/kg), gastric infusion, the administration volume is all with 0.15ml/10g mice weighing machine, and each treated animal is by above-mentioned dosage successive administration 3d; After administration in the 3rd day, mice was fixed in 1 hour, mouse tail 1cm place is cut off, treat that blood overflows voluntarily to pick up counting, inhale to dehematize with filter paper every 30s and drip 1 time, stop (when filter paper is inhaled till the depletion of blood) naturally, be the bleeding time until blood with operating scissors.Each administration group and matched group are compared, organize a t check.The results are shown in Table 4:
The influence that table 4 pair mice is hemorrhage
| Group | Dosage (g/kg) | Bleeding time (second) |
| Matched group | --- | 14.3±3.6 |
| The YUNNAN BAIYAO group | 0.2 | 7.8±3.7** |
| Herba Ecliptae dry product extract group | 1.0 | 13.2±3.9 |
| The bright product extract of Herba Ecliptae group | 1.0 | 8.6±4.8** |
P＜0.05, * * p＜0.01 (comparing) with matched group.
The result shows: the bright product extract of Herba Ecliptae can significantly shorten the bleeding time of mice, and the dry product extract does not possess this pharmacological action.
Conclusion: above-mentioned four experimental results show that the bright product extract of Herba Ecliptae has tangible analgesia, antiinflammatory action to mice, has significant Blood clotting, can significantly shorten the bleeding time of mice; Herba Ecliptae dry product extract does not then have these pharmacological actions.
The bright product of Herba Ecliptae have analgesia, antiinflammatory, blood coagulation and shorten the pharmacological actions such as bleeding time of mice, and the Herba Ecliptae dry product does not then possess these effects.Why both have so difference, main cause is: Herba Ecliptae performance analgesia, antiinflammatory, blood coagulation and shorten the pharmacological actions such as bleeding time of mice material base, be that effective ingredient is some heat-sensitive substances, the high temperature heating can make its effective ingredient destroyed, thereby still loses corresponding pharmacological action.And the employing microwave low-temperature drying can guarantee that the thermal sensitivity composition is not destroyed on the one hand, and on the other hand, microwave low-temperature drying has fast, makes things convenient for, loses advantages such as few with respect to other traditional drying modes.Select the low-temperature microwave drying when therefore, the present invention prepares Herba Ecliptae aquatic foods product extract for use.For further determining baking temperature, the applicant has carried out multiple comparison test, and result of the test shows that the extract effect that baking temperature obtains when being 20-30 ℃ is best.Therefore, the present invention selects for use 20-30 ℃ of microwave drying mode to prepare Herba Ecliptae extract.
Compared with prior art, Herba Ecliptae extract of the present invention is a raw material with the bright product of Herba Ecliptae, adopt the preparation of microwave low temperature (20-30 ℃) drying means, can fully guarantee the integrity of its active component, and technology is simple, do not need to introduce any organic reagent, thereby and can avoid the destroyed curative effect of medication that influences of thermal sensitivity ingredient.In addition, the application mode of extract of the present invention is more simple, safety, can obviously reduce use cost and production cost.
The specific embodiment:
Embodiments of the invention 1: the preparation of Herba Ecliptae extract:
Get the bright product 10kg of clean Herba Ecliptae, smash to pieces,, pulverize, cross 100 mesh sieves, promptly get Herba Ecliptae extract 1000g with microwave drying (20-30 ℃).
Embodiments of the invention 2: the purposes 1 of Herba Ecliptae extract:
The preparation of Herba Ecliptae gel: take by weighing Herba Ecliptae extract 200g, glycerol 50ml, propylene glycol 50ml, carbomer 60g, gelatin 10g grinds evenly, adds water and makes 400ml Herba Ecliptae gel.Get in right amount at every turn, be applied in the affected part, be used for the treatment of traumatic injury, traumatic hemorrhage.
Embodiments of the invention 3: the purposes 2 of Herba Ecliptae extract:
The preparation of Herba Ecliptae spray: take by weighing Herba Ecliptae extract 50g, ethanol 100ml, sorbester p37-2g, mix homogeneously adds an amount of propellant (as dimethyl ether, third butane), press-fits in bomb, promptly.Each an amount of, be sprayed on the affected part, be used for the treatment of traumatic injury, traumatic hemorrhage.
Embodiments of the invention 4: the purposes 3 of Herba Ecliptae extract:
The preparation of Herba Ecliptae unguentum: take by weighing Herba Ecliptae extract 100g, hydroxyphenyl Ethyl formate 0.3g, lanoline 50g adds vaseline to the 1000g mixing, promptly.Each an amount of, be applied in the affected part, be used for the treatment of traumatic injury, traumatic hemorrhage.
Claims (7)
1. the preparation method of a Herba Ecliptae extract is characterized in that: the Herba Ecliptae herb of fresh collection is removed impurity, clean, smash to pieces, 20-30 ℃ of microwave drying pulverized, and promptly gets Herba Ecliptae extract.
2. the preparation method of Herba Ecliptae extract is extracted the Herba Ecliptae extract that obtains according to claim 1.
3. Herba Ecliptae extract as claimed in claim 2 is used to prepare the Chinese medicine preparation of treatment traumatic injury, traumatic hemorrhage.
4. according to the purposes of the described Herba Ecliptae extract of claim 3, it is characterized in that: described Chinese medicine preparation is gel, spray or unguentum.
5. according to the purposes of claim 3 or 4 described Herba Ecliptae extracts, it is characterized in that: described gel adds water by Herba Ecliptae extract 200g, glycerol 50ml, propylene glycol 50ml, carbomer 60g and gelatin 10g and makes
6. according to the purposes of claim 3 or 4 described Herba Ecliptae extracts, it is characterized in that: described spray adds an amount of propellant by Herba Ecliptae extract 50g, ethanol 100ml and Arlacel-85 2g and makes.
7. according to the purposes of claim 3 or 4 described Herba Ecliptae extracts, it is characterized in that: described unguentum adds vaseline by Herba Ecliptae extract 100g, hydroxyphenyl Ethyl formate 0.3g and lanoline 50g and makes.
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| CN 200610201098 CN1994351A (en) | 2006-11-17 | 2006-11-17 | Yeradetajo extract, its preparation method and use |
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| CN 200610201098 CN1994351A (en) | 2006-11-17 | 2006-11-17 | Yeradetajo extract, its preparation method and use |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101856380A (en) * | 2010-06-29 | 2010-10-13 | 浙江大学 | Method for preparing active ingredient of yerbadetajo herb and application |
| CN101559090B (en) * | 2008-04-17 | 2010-11-17 | 霸王（广州）有限公司 | Extracting method of steroid saponins of yerbadetajo |
| CN104387404A (en) * | 2014-12-04 | 2015-03-04 | 陕西嘉禾植物化工有限责任公司 | Method for extracting and separating wedelolactone from herba ecliptae |
-
2006
- 2006-11-17 CN CN 200610201098 patent/CN1994351A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101559090B (en) * | 2008-04-17 | 2010-11-17 | 霸王（广州）有限公司 | Extracting method of steroid saponins of yerbadetajo |
| CN101856380A (en) * | 2010-06-29 | 2010-10-13 | 浙江大学 | Method for preparing active ingredient of yerbadetajo herb and application |
| CN104387404A (en) * | 2014-12-04 | 2015-03-04 | 陕西嘉禾植物化工有限责任公司 | Method for extracting and separating wedelolactone from herba ecliptae |
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Open date: 20070711 |