CN104387404A - Method for extracting and separating wedelolactone from herba ecliptae - Google Patents
Method for extracting and separating wedelolactone from herba ecliptae Download PDFInfo
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Abstract
The invention relates to a method for extracting and separating wedelolactone from herba ecliptae. The method comprises the following steps: primary extraction, secondary extraction, tandem column separation and crystallization and is characterized by grinding a raw material herba ecliptae, carrying out extraction, carrying out tandem column separation and then carrying out crystallization, thus obtaining required wedelolactone. The method for extracting wedelolactone from the raw material herba ecliptae is simple to operate, has the advantages of high product content and high recovery rate and is suitable for industrial production.
Description
Technical field
The invention belongs to coumarin kind compound extraction and isolation field, relate to a kind of method of extraction and isolation Wedelolactone, be specifically related to a kind of method of extraction and isolation Wedelolactone from Yerbadetajo Herb.
Background technology
The dry aerial parts that Yerbadetajo Herb (Her ba Eclipta e) is composite family Eclipta prostrata platymiscium Eclipta prostrata, being commonly called as Herba Ecliptae, is one of conventional Chinese medicine, has the function of nourishing liver and kidney, cooling blood for hemostasis.Recent domestic research finds that Yerbadetajo Herb has stronger Hepatocyte protection, if Wagner etc. is by finding that to the research of Yerbadetajo Herb chemical composition the rat hepatocytes toxicity of its Coumarether composition to tetracol phenixin and amino lactose has obvious Liver protection and promotion regeneration.The research of the domestic and international chemical composition to Yerbadetajo Herb and pharmacology aspect is more at present, but to Wedelolactone research fewer.
The structural formula of Wedelolactone is as follows:
Application number is 200610201098.2, Herba Ecliptae extract and its production and use, and this invention removes impurity with fresh Yerbadetajo Herb herb, cleans, smashs to pieces, 20-30 DEG C of microwave drying, pulverizes, to obtain final product.
Application number is 200810031092.4, a kind of extracting method of Yerbadetajo Herb oside compound, this invention adopts ethanol microwave extraction, foam counter-flow chromatogram prepares Total saponin, macroporous resin is separated, reverse osmosis membrane filters the Total saponin obtained, further removal of impurities, obtains high-purity total saponins compound.
Application number is 201010603861.0, a kind of method of extraction and isolation Wedelolactone from Yerbadetajo Herb, the method adopts supercritical fluid extraction, n-butanol extraction, be concentrated into the 1/15-1/8 of original volume, polyamide resin is separated, chloroform-dehydrated alcohol mixed solvent wash-out wash-out, collects elutriant evaporate to dryness and namely obtains product.
Application number is 201110184589.1, a kind of Preparation method and use of Herba Ecliptae extract, and this invention adopts extraction using alcohol, and macroporous resin is separated, and Fractional Collections, obtains product.In this invention, products obtained therefrom is saponin and flavones ingredient and Wedelolactone thereof, total triterpene compounds.
Application number is 201110187513.4, a kind of method for separating and preparing of Yerbadetajo Herb monomer component, this invention utilizes Yerbadetajo Herb alcohol extracting, silica gel column chromatography, chloroform-methanol wash-out, high speed adverse current chromatogram is separated the monomer obtaining eclalbasaponins I, eclalbasaponins II, eclalbasaponins III tri-kinds of effective constituents.
Application number is dynamic continuous circulation extracting method and the device of total triterpene saponin(e in 201310002410.5 Yerbadetajo Herb medicinal materials, and the method comprises raw material micronizing, alcohol extracting, and continuous circulation extracts.
People is had to utilize ethanol room temperature or heating to extract in foreign language, extracting solution concentrating under reduced pressure becomes medicinal extract, each position is taken out with opposed polarity organic solvent extracting, be separated in Semi-polarity position (ethyl acetate, ether, ether-methanol 4:1, n-butanol portion etc.) chromatography, purifying obtains Wedelolactone monomer.
Wagner etc. extract from Ealba L, and this technology is methyl alcohol soak extraction, concentrate to obtain medicinal extract, extraction into ethyl acetate, and be separated through C18 post, gradient elution obtains Wedelolactone.
Adopt water refluxing extraction in Japan's technology, butanols-water (1:1) adverse current-adverse current distribution, SephadexLH-20 post is separated.
From Yerbadetajo Herb raw material, extract the method for Wedelolactone in prior art, extraction step is complicated, and productive rate is low, is not suitable for suitability for industrialized production.
Summary of the invention
In order to solve the technical problem existing for background technology, the present invention proposes a kind of method extracting Wedelolactone from Yerbadetajo Herb raw material, the method is simple to operate, and product content is high, and the rate of recovery is high, is suitable for suitability for industrialized production.
Concrete technical scheme of the present invention is as follows:
A method for extraction and isolation Wedelolactone from Yerbadetajo Herb, comprises the following steps:
1] once extract
Pulverized by Yerbadetajo Herb, under 25-60 DEG C of condition, employing water or concentration are that the aqueous ethanolic solution of 1%-50% extracts 1-2h, extract 2-3 time, are then filtered dry by extraction slag;
2] second extraction
Extraction slag after being filtered dry is put in encloses container, under 20-60 DEG C of condition, with organic solvent extraction, in leaching process, adds antioxidant, extract 2-4h, then concentration;
Above-mentioned organic solvent is one or more mixing in sherwood oil, ethyl acetate, normal butane, acetone; Above-mentioned antioxidant is one or more mixing in BHT, TBHQ, Sodium Pyrosulfite, Sulfothiorine, VC; The throwing amount of above-mentioned antioxidant is the 0.01%-1% of raw material Yerbadetajo Herb quality;
3] columns in series is separated
Carry out two posts to step 2 gained concentrated solution to be separated, then process with eluting solvent, collect the elutriant containing effective constituent according to thin layer spot, be concentrated into the 1/100-1/10 of effluent volume, place crystallization;
Above-mentioned two posts comprise pretreatment column and conventional column; Above-mentioned pretreatment column diameter is 10cm-50cm, and height is 50cm; Described conventional column is 40cm × 400cm; Above-mentioned pretreatment column and the filler of conventional column be aluminum oxide, silica gel, MM500, Sephadex LH-200, C18,001 × 7,201 × 7, one or both in D204, Lx-8, LSA-10; Above-mentioned eluting solvent is one or more mixing in sherwood oil, ethyl acetate, normal butane, normal hexane, methyl alcohol, ethanol;
4] crystallization
By the crystallization of step 3 gained, by 6-10 times amount dissolution with solvents, be then concentrated into the 0.1-2 times amount of crystallization, under 100-1500r/min mechanical agitation, add the solvent of 1-4 times amount concentrated solution, treat that solution occurs muddy, place 6h-8h crystallization, crystallization filtered, drying both obtained Wedelolactone;
Above-mentioned solvent is acetone, ethyl acetate, methyl alcohol, ethanol, propyl carbinol; Above-mentioned organic solvent is one or more mixing of sherwood oil, normal butane, hexane, ethyl acetate.
In above-mentioned steps 2, extraction slag after being filtered dry is dropped in encloses container, under 25 DEG C of conditions, with normal butane and the extraction of ethyl acetate mixed solvent that mass ratio is 19:1, what in leaching process, add raw material Yerbadetajo Herb 0.1% times amount is the Sodium Pyrosulfite of 1:5 and the mixing antioxidant of VC in mass ratio, extract 4h, then concentration.
In above-mentioned steps 3, carry out two posts to step 2 gained concentrated solution to be separated, one of them column packing is the pretreatment column 30cm × 50cm of aluminum oxide, another one filler is the conventional column 40cm × 400cm of MM500, then with mass ratio be the normal butane of 45:4:1, the mixing eluting solvent of sherwood oil and ethanol processes, collect the elutriant containing effective constituent according to thin layer spot, be concentrated into 1/30 of effluent volume, place crystallization.
In above-mentioned steps 4, by the crystallization of step 3 gained, with the dissolve with methanol of 6-10 times amount, be then concentrated into 0.35 times amount of crystallization, under 1300r/min mechanical agitation, the mass ratio adding 1-4 times amount concentrated solution is the sherwood oil of 8.2:2.8 and the mixing solutions of ethyl acetate, treat that solution occurs muddy, place 8h crystallization, crystallization is filtered, drying, obtains Wedelolactone.
Advantage of the present invention is as follows:
1, due to the character of Wedelolactone, can, by the Impurity removal of large for part polarity, be convenient to follow-up refining;
2, yield is high, and process is simple, and easy to operate, product Wedelolactone content reaches more than 98%;
3, the utilization of columns in series and a large amount of screenings of filler, vital effect is played in the separation for Wedelolactone.
Accompanying drawing explanation
Fig. 1 is the chromatograms of prior art;
Fig. 2 is chromatograms of the present invention;
Fig. 3 is the chromatograms of the chemical composition of Yerbadetajo Herb.
Embodiment
Embodiment one
10kg Yerbadetajo Herb raw material after crushed, adopts water extraction 1h, extracts 2 times, temperature 25 DEG C.Filtrate is abandoned, and extracts slag and is filtered dry.
Extracting slag adopts the sherwood oil of 5 times amount mass ratio 9:1 and the mixed solvent of ethyl acetate to extract, and Extracting temperature is the BHT adding raw materials quality 0.01% in 20 DEG C of leaching process, extracts 2h, concentrated for subsequent use.
Pretreatment column diameter is 10cm, height is 50cm, filler used is aluminum oxide, conventional column filler is silica gel and C18, and its mass ratio is 1:1, and eluting solvent is the mixed solvent of normal hexane and ethyl acetate, its mass ratio is 4:1, collect the elutriant containing effective constituent, be concentrated into 1/100 of elutriant, place crystallization.
By crystallized sample, after adopting the acetic acid ethyl dissolution of 10 times of crystalline qualities, be concentrated into 2 times amount of crystalline quality, under mechanical agitation, stirring velocity is 100r/min, add the normal butane of concentrated solution 4 times of quality, be stirred to solution muddy a little, place crystallization 6h, crystallization filtration drying, products obtained therefrom is 20.0g, and content is the Wedelolactone of 98.4%.
Embodiment two
10kg Yerbadetajo Herb raw material after crushed, adopts 5% extraction using alcohol 1.5h, extracts 2 times, temperature 60 C.Filtrate is abandoned, and extracts slag and is filtered dry.
Extracting slag adopts the ethyl acetate of 5 times of raw materials quality to extract, and Extracting temperature is 60 DEG C, adds the Sodium Pyrosulfite of raw materials quality 1% in leaching process, extracts 4h, concentrated for subsequent use.
Pretreatment column diameter is 50cm, and height is 50cm, and filler used is D204, and conventional column filler is MM500, and eluting solvent is sherwood oil: ethyl acetate: methyl alcohol=4:1:3, collects the elutriant containing effective constituent, is concentrated into 1/10 of elutriant, places crystallization.
By crystallized sample, after adopting the acetone solution of 6 times of crystalline qualities, be concentrated into 0.1 times amount of crystalline quality, under mechanical agitation, stirring velocity is 1200r/min, add the sherwood oil of concentrated solution 1 times of quality, being stirred to solution occurs muddy, places crystallization 6h, crystallization filtration drying, products obtained therefrom is 24g, and content is the Wedelolactone of 98.8%.
Embodiment three
100kg Yerbadetajo Herb raw material after crushed, adopts 50% extraction using alcohol 2h, extracts 2 times, temperature 30 DEG C.Filtrate is abandoned, and extracts slag and is filtered dry.
Extracting slag adopts the normal butane of 4 times of raw materials quality to extract, and Extracting temperature is 40 DEG C, adds the mixing antioxidant of raw materials quality 0.1% in leaching process, its antioxidant is the mixing antioxidant of Sodium Pyrosulfite and VC, mass ratio both it is 1:5, extracts 3h, concentrated for subsequent use.
Pretreatment column diameter is 20cm, and height is 50cm, and filler used is 001 × 7, and conventional column filler is silica gel, and eluting solvent is ethyl acetate: ethanol=95:5, collects the elutriant containing effective constituent, is concentrated into 1/50 of elutriant, places crystallization.
By crystallized sample, after adopting the dissolve with methanol of 8 times of crystalline qualities, be concentrated into 0.5 times amount of crystalline quality, under mechanical agitation, stirring velocity is 1500r/min, add the hexane of concentrated solution 4 times of quality, being stirred to solution occurs muddy, places crystallization 6h, crystallization filtration drying, products obtained therefrom is 290g, and content is the Wedelolactone of 98.6%.
Embodiment four
1000kg Yerbadetajo Herb raw material after crushed, adopts 40% extraction using alcohol 1.5h, and extract 2 times, temperature 35 DEG C, filtrate is abandoned, and extracts slag and is filtered dry.
Extract the acetone extraction that slag adopts 5 times of raw materials quality, Extracting temperature is 50 DEG C, adds the VC of raw materials quality 1% in leaching process, extracts 2h, concentrated for subsequent use.
Pretreatment column diameter is 40cm, and height is 50cm, and filler used is aluminum oxide, conventional column filler is MM500, and eluting solvent is the mixed solvent of normal butane, sherwood oil, ethanol, and its mass ratio is 90:8:2, collect the elutriant containing effective constituent, be concentrated into 1/30 of elutriant, place crystallization.
By crystallized sample, after adopting the dissolve with ethanol of 7.5 times of crystalline qualities, be concentrated into 0.3 times amount of crystalline quality, under mechanical agitation, stirring velocity is 1300r/min, add the normal butane of concentrated solution 2.8 times of quality, being stirred to solution occurs muddy, places crystallization 6h, crystallization filtration drying, products obtained therefrom is 2950g, and content is the Wedelolactone of 99.6%.
Embodiment five
1000kg Yerbadetajo Herb raw material after crushed, adopts 45% extraction using alcohol 1.5h, extracts 2 times, temperature 45 C.Filtrate is abandoned, and extracts slag and is filtered dry.
Extracting slag adopts the normal butane of 5 times of raw materials quality and the mixed solvent of ethyl acetate to extract, and its mass ratio is 95:5, and Extracting temperature is 25 DEG C, adds the BHT of raw materials quality 0.2% in leaching process, extracts 2h, concentrated for subsequent use.
Pretreatment column diameter is 30cm, height is 50cm, filler used is LSA-10, conventional column filler is the mixed fillers of silica gel and MM500, and its mass ratio is 2:1, and eluting solvent is the mixed solvent of sherwood oil, ethyl acetate, normal butane, its mass ratio is 8:1:3, collect the elutriant containing effective constituent, be concentrated into 1/40 of elutriant, place crystallization.
By crystallized sample, after adopting the dissolve with methanol of 10 times of crystalline qualities, be concentrated into 0.35 times amount of crystalline quality, under mechanical agitation, stirring velocity is 1000r/min, adds the mixed solvent of concentrated solution 2.3 times of quality, and its solvent is sherwood oil and ethyl acetate, its ratio is 8.2:2.8, being stirred to solution occurs muddy, places crystallization 8h, crystallization filtration drying, products obtained therefrom is 2982g, and content is the Wedelolactone of 98.0%.
Embodiment six
1000kg Yerbadetajo Herb raw material after crushed, adopts 30% extraction using alcohol 1.5h, extracts 3 times, temperature 40 DEG C.Filtrate is abandoned, and extracts slag and is filtered dry.
Extracting slag adopts the normal butane of 5 times of raw materials quality and the mixed solvent of ethyl acetate to extract, mass ratio is 8:1, Extracting temperature is 45 DEG C, the mixing antioxidant of raw materials quality 0.5% is added in leaching process, its antioxidant is the mixed solvent of Sulfothiorine and TBHQ, the mass ratio of the two is=6:4, extracts 4h, concentrated for subsequent use.
Pretreatment column diameter is 40cm, height is 50cm, filler used is Lx-8, conventional column filler is the mixed fillers of aluminum oxide and MM500, and its mass ratio is 1:3, and eluting solvent is the mixed solvent of normal hexane and methyl alcohol, its mass ratio is 98:2, collect the elutriant containing effective constituent, be concentrated into 1/10 of elutriant, place crystallization.
By crystallized sample, after adopting the dissolve with ethanol of 7.5 times of crystalline qualities, be concentrated into 0.45 times amount of crystallized sample, under mechanical agitation, stirring velocity is 800r/min, add the normal butane of concentrated solution 2 times of quality and the mixing solutions of ethyl acetate, the ratio both it is 3:7, is stirred to solution and occurs muddy, place 6h crystallization, crystallization filtration drying, products obtained therefrom is 3012g, and content is the Wedelolactone of 98.7%.
Embodiment seven
1000kg Yerbadetajo Herb raw material after crushed, adopts 42% extraction using alcohol 1h, extracts 2 times, temperature 38 DEG C.Filtrate is abandoned, and extracts slag and is filtered dry.
Extract the mixed solvent that slag adopts the acetoneand ethyl acetate of 4 times amount, its mass ratio is 1:1, and Extracting temperature is 45 DEG C, the Sodium Pyrosulfite of raw materials quality 0.8% and the mixing antioxidant of VC is added in leaching process, mass ratio 3:7 both it, extracts 2h, concentrated for subsequent use.
Pretreatment column diameter is 30cm, height is 50cm, filler used is Sephadex LH-200, conventional column filler is the mixed fillers of C18 and MM500, and its mass ratio is 1:1.5, and eluting solvent is the mixed solvent of normal butane, sherwood oil, ethanol, its mass ratio is 80:8:12, collect the elutriant containing effective constituent, be concentrated into 1/30 of elutriant, place crystallization.
By crystallized sample, after adopting the acetone solution of 6 times of crystalline qualities, be concentrated into 1 times amount of crystalline quality, under mechanical agitation, stirring velocity is 600r/min, add the normal butane of concentrated solution 1.8 times of quality and the mixed solvent of hexane, its mass ratio is 9:1, is stirred to solution and occurs muddy, place crystallization 6h, crystallization filtration drying, products obtained therefrom is 2982g, and content is the Wedelolactone of 98.6%.
Embodiment eight
1000kg Yerbadetajo Herb raw material after crushed, adopts 45% extraction using alcohol 1.5h, extracts 2 times, temperature 45 C.Filtrate is abandoned, and extracts slag and is filtered dry.
Extracting slag adopts the normal butane of 5 times of raw materials quality and the mixed solvent of ethyl acetate to extract, and its mass ratio is 19:1, and Extracting temperature is 25 DEG C, the Sodium Pyrosulfite of raw materials quality 0.1% and the mixing antioxidant of VC is added in leaching process, mass ratio both it is 1:5, extracts 4h, concentrated for subsequent use.
Pretreatment column diameter is 30cm, and height is 50cm, and filler used is aluminum oxide, conventional column filler is MM500, and eluting solvent is the mixed solvent of normal butane, sherwood oil, ethanol, and its mass ratio is 45:4:1, collect the elutriant containing effective constituent, be concentrated into 1/40 of elutriant, place crystallization.
By crystallized sample, after adopting the dissolve with methanol of 8 times of crystalline qualities, be concentrated into 0.35 times amount of crystalline quality, under mechanical agitation, stirring velocity is 1000r/min, adds the mixed solvent of concentrated solution 2.5 times of quality, and its solvent is sherwood oil and ethyl acetate, its ratio is 8.2:2.8, being stirred to solution occurs muddy, places crystallization 6h, crystallization filtration drying, products obtained therefrom is 3032g, and content is the Wedelolactone of 99.0%.
Can draw through abundant experimental results, from Yerbadetajo Herb, should be more than or equal to 0.20% by the yield of the method products obtained therefrom of extraction and isolation Wedelolactone, in product, Wedelolactone content is more than or equal to 98.0%; Therefore, the method product content is high, and the rate of recovery is high, is suitable for suitability for industrialized production.
The present invention and prior art carry out experimental result contrast, and draw following result:
From collection of illustrative plates comparing result, in prior art, extracting method (i.e. CN201010603861.0) also has an impurity collection of illustrative plates in back, Yerbadetajo Herb lactone peak, and the impurity collection of illustrative plates impurity amount of accounting for of Fig. 2 and Fig. 3 is little.
Claims (4)
1. the method for extraction and isolation Wedelolactone from Yerbadetajo Herb, is characterized in that, comprise the following steps:
1] once extract
Pulverized by Yerbadetajo Herb, under 25-60 DEG C of condition, employing water or concentration are that the aqueous ethanolic solution of 1%-50% extracts 1-2h, extract 2-3 time, are then filtered dry by extraction slag;
2] second extraction
Extraction slag after being filtered dry is put in encloses container, under 20-60 DEG C of condition, with organic solvent extraction, in leaching process, adds antioxidant, extract 2-4h, then concentration;
Described organic solvent is one or more mixing in sherwood oil, ethyl acetate, normal butane, acetone; Described antioxidant is one or more mixing in BHT, TBHQ, Sodium Pyrosulfite, Sulfothiorine, VC; The throwing amount of described antioxidant is the 0.01%-1% of raw material Yerbadetajo Herb quality;
3] columns in series is separated
Carry out two posts to step 2 gained concentrated solution to be separated, then process with eluting solvent, collect the elutriant containing effective constituent according to thin layer spot, be concentrated into the 1/100-1/10 of effluent volume, place crystallization;
Described two posts comprise pretreatment column and conventional column; Described pretreatment column diameter is 10cm-50cm, and height is 50cm; Described conventional column is 40cm × 400cm; Described pretreatment column and the filler of conventional column be aluminum oxide, silica gel, MM500, Sephadex LH-200, C18,001 × 7,201 × 7, one or both in D204, Lx-8, LSA-10; Described eluting solvent is one or more mixing in sherwood oil, ethyl acetate, normal butane, normal hexane, methyl alcohol, ethanol;
4] crystallization
By the crystallization of step 3 gained, by 6-10 times amount dissolution with solvents, be then concentrated into the 0.1-2 times amount of crystallization, under 100-1500r/min mechanical agitation, add the solvent of 1-4 times amount concentrated solution, treat that solution occurs muddy, place 6h-8h crystallization, crystallization filtered, drying both obtained Wedelolactone;
Described solvent is acetone, ethyl acetate, methyl alcohol, ethanol, propyl carbinol; Described organic solvent is one or more mixing of sherwood oil, normal butane, hexane, ethyl acetate.
2. the method for extraction and isolation Wedelolactone from Yerbadetajo Herb according to claims 1, it is characterized in that: in described step 2, extraction slag after being filtered dry is dropped in encloses container, under 25 DEG C of conditions, with normal butane and the extraction of ethyl acetate mixed solvent that mass ratio is 19:1, what in leaching process, add raw material Yerbadetajo Herb 0.1% times amount is the Sodium Pyrosulfite of 1:5 and the mixing antioxidant of VC in mass ratio, extracts 4h, then concentration.
3. the method for extraction and isolation Wedelolactone from Yerbadetajo Herb according to claims 1, it is characterized in that: in described step 3, carry out two posts to step 2 gained concentrated solution to be separated, one of them column packing is the pretreatment column 30cm × 50cm of aluminum oxide, another one filler is the conventional column 40cm × 400cm of MM500, then with mass ratio be the normal butane of 45:4:1, the mixing eluting solvent of sherwood oil and ethanol processes, the elutriant containing effective constituent is collected according to thin layer spot, be concentrated into 1/30 of effluent volume, place crystallization.
4. the method for extraction and isolation Wedelolactone from Yerbadetajo Herb according to claims 1, it is characterized in that: in described step 4, by the crystallization of step 3 gained, with the dissolve with methanol of 8 times amount, then 0.35 times amount of crystallization is concentrated into, under 1000r/min mechanical agitation, the mass ratio adding 2.5 times amount concentrated solutions is the sherwood oil of 8.2:2.8 and the mixing solutions of ethyl acetate, treats that solution occurs muddy, place 6h crystallization, crystallization is filtered, dry, obtain Wedelolactone.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1994351A (en) * | 2006-11-17 | 2007-07-11 | 贵州师范大学 | Yeradetajo extract, its preparation method and use |
| CN102247420A (en) * | 2011-07-04 | 2011-11-23 | 浙江大学 | Preparation method and application of eclipta extract |
| CN103951672A (en) * | 2014-04-08 | 2014-07-30 | 天津大学 | Method for extracting wedelolactone and method for determining solubility of wedelolactone |
-
2014
- 2014-12-04 CN CN201410735341.3A patent/CN104387404B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1994351A (en) * | 2006-11-17 | 2007-07-11 | 贵州师范大学 | Yeradetajo extract, its preparation method and use |
| CN102247420A (en) * | 2011-07-04 | 2011-11-23 | 浙江大学 | Preparation method and application of eclipta extract |
| CN103951672A (en) * | 2014-04-08 | 2014-07-30 | 天津大学 | Method for extracting wedelolactone and method for determining solubility of wedelolactone |
Non-Patent Citations (1)
| Title |
|---|
| LU HAN ET AL.: "Herba Ecliptae Protects against Hydroxyl Radical-induced Damages to DNA and Mesenchymal Stem Cells via Antioxidant Mechanism", 《JOURANL OF THE CHINESE CHEMICAL SOCIETY》, vol. 61, 26 June 2014 (2014-06-26), pages 1161 - 1167 * |
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Address after: 710075 Xi'an high tech Zone, Shaanxi hi tech Road, No. 25 Maple Leaf Garden District, block C, No. 157 Applicant after: SHAANXI JIAHE PHYTOCHEM CO., LTD. Address before: 710075 Xi'an high tech Zone, Shaanxi hi tech Road, No. 25 Maple Leaf Garden District, block C, No. 157 Applicant before: Shanxi Jiahe Plant Chemical Co., Ltd. |
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Address after: 710075 Xi'an high tech Zone, Shaanxi hi tech Road, No. 25 Maple Leaf Garden District, block C, No. 157 Applicant after: SHAANXI JIAHE PHYTOCHEM CO., LTD. Address before: 710075 Xi'an high tech Zone, Shaanxi hi tech Road, No. 25 Maple Leaf Garden District, block C, No. 157 Applicant before: Shanxi Jiahe Plant Chemical Co., Ltd. |
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