CN1987424A - 油脂羰基值的测定方法 - Google Patents

油脂羰基值的测定方法 Download PDF

Info

Publication number
CN1987424A
CN1987424A CN 200510122498 CN200510122498A CN1987424A CN 1987424 A CN1987424 A CN 1987424A CN 200510122498 CN200510122498 CN 200510122498 CN 200510122498 A CN200510122498 A CN 200510122498A CN 1987424 A CN1987424 A CN 1987424A
Authority
CN
China
Prior art keywords
solvent
carbonyl value
benzene
oil
generates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN 200510122498
Other languages
English (en)
Inventor
李昌模
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tianjin University of Science and Technology
Original Assignee
Tianjin University of Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tianjin University of Science and Technology filed Critical Tianjin University of Science and Technology
Priority to CN 200510122498 priority Critical patent/CN1987424A/zh
Publication of CN1987424A publication Critical patent/CN1987424A/zh
Pending legal-status Critical Current

Links

Images

Landscapes

  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

以往测定油脂氧化、劣败的指标之一的羰基值测定的方法使用有毒性的溶剂苯，使用溶剂量大且操作繁琐，时间长等不适用于油脂食品工厂的现场使用。本发明的目的是使用安全、准确、快速的检测方法应用于实际油脂加工现场。本发明以安全的正丁醇为有机溶剂，利用油脂中的羰基化合物与2，4－二硝基苯肼反应生成2，4－二硝基苯腙。2，4－二硝基苯腙在碱性条件下生成葡萄酒红色的醌，此颜色在380nm－420nm处具有吸光特性利用比色法进行定量分析。

Description

油脂羰基值的测定方法
技术领域
油脂(动物油，植物油，矿物油)发生氧化、劣败时生成挥发性和不挥发性羰基化合物。羰基化合物的增加影响油脂及其油脂加工食品的色、香、味。所以油脂中存在的羰基化合物含量是判断油脂氧化、劣败的重要指标。
背景技术
以往测定羰基值的方法使用有毒性的容剂(苯)，将油样和2，4-二硝基苯肼溶解在苯溶剂中在60度下加热反应30分钟，在碱性条件下的显色反应通过在440nm处吸光度利用比色法测定羰基值。此方法使用溶剂量大且操作繁琐，时间长等不适用于油脂食品工厂的现场使用。
发明内容
本发明使用安全无毒的正丁醇(还有异丁醇)为溶剂，反应在40度下进行20分钟，在碱性条件下显色反应后进行离心分离(3000rpm/min)。吸光度的测定是在380-420nm处。
附图说明
图一反应原理图(2，4-二硝基苯肼与羰基化合物反应生成2，4-二硝基苯腙，2，4-二硝基苯腙在碱性条件下生成具有葡萄酒红色的醌类化合物)
图二利用饱和醛，一价不饱和醛，二价不饱和醛在相同浓度下的交叉确定测定波长范围
图三正己醛标准曲线的吸光系数(斜率)作为计算羰基值的吸光系数
图四传统方法与新方法测定羰基值的比较
具体实施方式
称取油样0.04～1.0g放入10ml容量瓶中用正丁醇溶液定容。提取1mL样品液放入15ml离心试管中，加入1ml 2，4-二硝基苯肼溶液(容积为正丁醇)，盖上塞子，摇匀，在40℃水浴中加热20min。反应后取出用流水冷却至室温，加入8ml 2.5％KOH正丁醇溶液使其显色。试管在3000rpm/min转速下离心5min，取上清液测其在380-420nm下的吸光度。

Claims (2)

  1. (一)前序
    主体名称  油脂羰基值的测定方法
    油脂中的羰基化合物和2，4-二硝基苯肼溶液在酸性条件下反应生成2，4 -二硝基苯腙，2，4-二硝基苯腙在碱性条件下生成具有葡萄酒红色的醌类化合物并以正己醛为标准物质通过利用比色法测定油脂的羰基值。
  2. (二)技术
    测定油脂羰基值的新方法使用正丁醇为溶剂，测定波长在380-420nm，与传统的苯法(440nm)的相关系数为0.8291。1，使用的溶剂----正丁醇；2，测定波长在380-420nm。
CN 200510122498 2005-12-21 2005-12-21 油脂羰基值的测定方法 Pending CN1987424A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200510122498 CN1987424A (zh) 2005-12-21 2005-12-21 油脂羰基值的测定方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200510122498 CN1987424A (zh) 2005-12-21 2005-12-21 油脂羰基值的测定方法

Publications (1)

Publication Number Publication Date
CN1987424A true CN1987424A (zh) 2007-06-27

Family

ID=38184304

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 200510122498 Pending CN1987424A (zh) 2005-12-21 2005-12-21 油脂羰基值的测定方法

Country Status (1)

Country Link
CN (1) CN1987424A (zh)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102841091A (zh) * 2012-08-27 2012-12-26 宋继增 工业甲醇中羰基化合物含量测定的标准比色法
CN104777124A (zh) * 2015-05-04 2015-07-15 川渝中烟工业有限责任公司 烟草中羰基化合物的测定方法
CN105277493A (zh) * 2015-09-23 2016-01-27 浙江大学宁波理工学院 检测dera酶催化醛缩反应液中氯乙醛含量及催化效率的方法

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102841091A (zh) * 2012-08-27 2012-12-26 宋继增 工业甲醇中羰基化合物含量测定的标准比色法
CN104777124A (zh) * 2015-05-04 2015-07-15 川渝中烟工业有限责任公司 烟草中羰基化合物的测定方法
CN105277493A (zh) * 2015-09-23 2016-01-27 浙江大学宁波理工学院 检测dera酶催化醛缩反应液中氯乙醛含量及催化效率的方法
CN105277493B (zh) * 2015-09-23 2018-05-01 浙江大学宁波理工学院 检测dera酶催化醛缩反应液中氯乙醛含量及催化效率的方法

Similar Documents

Publication Publication Date Title
Kakuda et al. Determination of TBA number by high performance liquid chromatography
Ke et al. Microdetermination of thiobarbituric acid values in marine lipids by a direct spectrophotometric method with a monophasic reaction system
Morse Anthrone in estimating low concentrations of sucrose
CN105021608A (zh) 用于检测饮品总多酚含量的试剂盒、制备方法和使用试剂盒进行检测的方法
US4019862A (en) CO2 measurement and reagents therefor
Seiler et al. Development of an ethanol-selective optode membrane based on a reversible chemical recognition process
CN102706872A (zh) 一种食用油脂过氧化值检测组合试剂及其检测方法
CN1987424A (zh) 油脂羰基值的测定方法
Fishman et al. Indirect Spectrophotometric Determination of Traces of Bromide in Water.
CN104492511A (zh) 一种二氧化硫快速检测试剂管及其检测方法
CN101000310A (zh) 一种检测甲醛的方法
Gryllaki‐Berger et al. A comparative study of formaldehyde detection using chromotropic acid, acetylacetone and HPLC in cosmetics and household cleaning products
Schibsted New test for fat aldehydes resulting from oxidation of fats and oils
CN101046443A (zh) 油脂中羰基化合物含量的测定新方法
Hirayama et al. High performance liquid chromatographic determination of malondialdehyde in vegetable oils
Attaway et al. Methods for the determination of oxygenated terpene, aldehyde, and ester concentrations in aqueous citrus essences
Irwin On the nature of the dye penetrating the vacuole of Valonia from solutions of methylene blue
JP2007139558A (ja) 油脂のカルボニル価の測定方法及びカルボニル価測定組成物
US3552926A (en) Process for continuous analysis of nitroglycerin and related nitrate esters
Roy et al. Automated analysis of sorbic acid in food products
Stanley et al. Citral determination, specific quantitative colorimetric method of analysis for citral in lemon oil
CN100439901C (zh) 鉴别酿造食醋与配制食醋的试剂盒
Tawfik et al. Automated analytical method for the determination of individual sugars in mixtures of glucose, fructose and sucrose
Hiromi et al. A kinetic method for the determination of ascorbic acid with 2, 6-dichlorophenolindophenol
Isaacs Colorimetric determination of vitamin C

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication