CN1974741A - Process of extracting sturgeon oil - Google Patents
Process of extracting sturgeon oil Download PDFInfo
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- CN1974741A CN1974741A CN 200610124065 CN200610124065A CN1974741A CN 1974741 A CN1974741 A CN 1974741A CN 200610124065 CN200610124065 CN 200610124065 CN 200610124065 A CN200610124065 A CN 200610124065A CN 1974741 A CN1974741 A CN 1974741A
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Abstract
The process of extracting sturgeon oil includes the following steps: grinding sturgeon maw and internal organs, adding 3-4 times of water and small amount of antioxidant, enzymolysis, stilling to delaminate, separating to obtain coarse sturgeon oil, and deacidifying, deodorizing and defatting coarse sturgeon oil to obtain refined sturgeon oil. The process has high sturgeon oil extracting rate, low peroxide number of the sturgeon oil product, high product quality, capacity of obtaining fish protein enzymolyzing matter also with the enzymolysis liquid, and other advantages.
Description
Technical field
The present invention relates to the sturgeon oil new method for extracting, especially relate to the application of zymolysis technique in sturgeon oil extracts.
Background technology
Sturgeon also claims the sturgeon dragon, and the same with shark is the most ancient and primary cartilage fingerling on the earth, is the fish of individual maximum, longest-lived in the freshwater fish, has very high economic worth and scientific value.Along with the success and the popularization of sturgeon artificial propagation and cultural technique, China's sturgeon cultivation had been obtained significant achievement already, cultured area of sturgeon and cultured output cumulative year after year, and the sturgeon comprehensive utilizating research becomes the hot issue that the Recent study personnel pay close attention to.
The bilgy odour of delicate, the no fresh water fingerling of sturgeon meat, delicious flavour just is regarded as treasure in the water since ancient times, is subjected to human consumer's favor deeply.The sturgeon seed is the transparent round granular solid of chocolate, have the title of " black gold ", and for adult sturgeon, about 25% of the common percentage of liveweight of its roe content is the very important precious resources of sturgeon processing enterprise.Therefore sturgeon processing at present is mainly based on sturgeon seed and sturgeon, and other parts are many loses as waste, not only causes the waste of resource also to pollute the environment.
Especially the processing and utilization at position such as sturgeon internal organ and sturgeon tripe is extremely rare, and present result of study shows the feature lipid acid of sturgeon internal organ and fish maw fish oil is to be rich in polyunsaturated fatty acid (Poly-unsaturated Fattyacids is PUFA) as EPA (timnodonic acid) and DHA (docosahexenoic acid).Lipid acid is closed in these many insatiable hungers can suppress growth of tumor, is essential to human foetus and newborn baby's neurodevelopment in vegetative period, has brain tonic and intelligence development, reducing blood-fat, anti-platelet aggregation and delay effects such as thrombotic.Therefore the extraction of sturgeon oil has exploitation value.
At present, the extracting method of China's fish oil is many with ammonia process, the potassium method, and cooking process is main, these methods can influence the quality of fish oil to a certain extent, are difficult to satisfy people's needs.Easily cause the oxidation of fish oil as cooking process; Contain a large amount of sylvite in the by product behind the potassium method extraction fish oil, environment is caused certain pollution; Ammonia process extracts fish oil and contains pungent ammonia flavor, therefore is necessary the fish oil extracting method is improved research.The research of this experiment adopts enzyme process to extract fish oil from the sturgeon internal organ, compares with traditional extracting method, and not only fish oil quality is better, and extraction yield is also high a lot.Thereby can save cost, and improve product production, improve manufacturing enterprise's economic benefit.
Summary of the invention
The object of the present invention is to provide the sturgeon oil extracting method, change traditional fish oil extraction process, it is preferable to extract fish oil extraction quality and quantity quality, promotes the lifting of its economic benefit.
For achieving the above object, extraction process of the present invention comprises the steps:
A, get fish maw and fish viscera and rub, add 3～4 times water and antioxidant of raw material weight, carry out enzymolysis processing, the solution left standstill layering behind the enzymolysis separates obtaining crude fish oil.
B, crude fish oil promptly get refined fish oil after depickling, deodorization, skimming treatment.
Used enzyme is a subtilisin among the described step a, and than enzyme 480～500U/g alive, consumption is 0.4～0.6% of a raw material weight
Used antioxidant is TBHQ, tea-polyphenol or Vc among the described step a, and add-on is 0.01% of raw material weight.
Enzymatic hydrolysis condition among the described step a is 7～8 for enzymolysis solution pH, and temperature 40-45 ℃, enzymolysis time is controlled at 2～3h.
This invention compared with prior art has following advantage: (1) the present invention decompose fish meat protein by zymolysis technique, the fat that is strapped in the muscle tissue is discharged, reach the purpose of extracting fish oil, this method can obviously improve the extraction yield of fish oil, the peroxide value of fish oil is starkly lower than ammonia process, potassium method and cooking process, and quality obviously is better than traditional fish oil extracting method.Avoid alkaline process and ammonia process to put forward salt residue problem in the oily process simultaneously, can not form new waste, and enzymolysis solution can also utilize once more and obtains the fish protein zymolyte.This law and traditional extraction process relatively see Table 1.
The different oil extracting methods of table 1 are to the influence of crude fish oil quality
| Method | Ammonia process | The potassium method | Cooking process | Enzyme process |
| Extraction yield % | 51.3 | 57.1 | 50.9 | 80.7 |
| Peroxide value | 0.36 | 0.41 | 0.78 | ＜0.30 |
| Color | Golden yellow | Golden yellow | Pale brown look | Light yellow |
| Smell | Light fishy smell, ammonia is highly seasoned | Light fishy smell | Denseer fishy smell | Light fishy smell |
| Transparency | Bright, small amount of crystal is arranged | Bright, small amount of crystal is arranged | Bright | Bright |
(2) this law adds antioxidant before hydrolysis, and the extraction yield of fish oil is not had influence, but has controlled the oxidising process of fish oil from the source, can obviously reduce the peroxide value of end product.The results are shown in Table 2.
The different antioxidants of table 2 are to the influence of fish oil extraction yield and peroxide value
| Antioxidant | TBHQ | Tea-polyphenol | Vc | Do not add antioxidant |
| Extraction yield % | 81.2 | 81.6 | 80.1 | 80.7 |
| Peroxide value | 0.15 | 0.23 | 0.21 | 0.75 |
Embodiment
Embodiment 1
Get fish maw and fish viscera and rub, add 3～4 times water and 0.01% TBHQ, subtilisin (than enzyme 480U/g alive) consumption is 0.4% of a raw material weight, and enzymatic hydrolysis condition is that pH is 7,40 ℃ of temperature, and enzymolysis time is controlled at 3h.Separate obtaining crude fish oil, wherein the crude fish oil yield is 80.9%, and peroxide value is 0.13.Crude fish oil promptly gets refined fish oil after depickling, deodorization, skimming treatment.
Embodiment 2
Get fish maw and fish viscera and rub, add 3～4 times water and 0.01% tea-polyphenol, subtilisin (than enzyme 480U/g alive) consumption is 0.5% of a raw material weight, and enzymatic hydrolysis condition is that pH is 7.5,45 ℃ of temperature, and enzymolysis time is controlled at 2.5h.Separate obtaining crude fish oil, wherein the crude fish oil yield is 82.3%, and peroxide value is 0.25.Crude fish oil promptly gets refined fish oil after depickling, deodorization, skimming treatment.
Embodiment 3
Get fish maw and fish viscera and rub, add 3～4 times water and 0.01% Vc, subtilisin (than enzyme 480U/g alive) consumption is 0.6% of a raw material weight, and enzymatic hydrolysis condition is that pH is 7.5,50 ℃ of temperature, and enzymolysis time is controlled at 2h.Separate obtaining crude fish oil, wherein the crude fish oil yield is 81.6%, and peroxide value is 0.19.Crude fish oil promptly gets refined fish oil after depickling, deodorization, skimming treatment.
Claims (4)
1, a kind of method of extracting sturgeon oil is characterized in that may further comprise the steps:
A, get fish maw and fish viscera and rub, add 3～4 times water and antioxidant of raw material weight, carry out enzymolysis processing, the solution left standstill layering behind the enzymolysis separates obtaining crude fish oil;
B, crude fish oil promptly get refined fish oil after depickling, deodorization, skimming treatment.
2, the method for extraction sturgeon oil according to claim 1 is characterized in that: used enzyme is a subtilisin among the described step a, and than enzyme 480～500U/g alive, consumption is 0.4～0.6% of a raw material weight.
3, the method for extraction sturgeon oil according to claim 1 is characterized in that: used antioxidant is TBHQ, tea-polyphenol or Vc among the described step a, and add-on is 0.01% of raw material weight.
4, the method for extraction sturgeon oil according to claim 1 is characterized in that: the enzymatic hydrolysis condition among the described step a is 7～8 for enzymolysis solution pH, and temperature 40-45 ℃, enzymolysis time is controlled at 2～3h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610124065 CN1974741A (en) | 2006-12-05 | 2006-12-05 | Process of extracting sturgeon oil |
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| CN 200610124065 CN1974741A (en) | 2006-12-05 | 2006-12-05 | Process of extracting sturgeon oil |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101824364A (en) * | 2010-04-09 | 2010-09-08 | 浙江兴业集团有限公司 | Method for deacidifying and refining high-acid value fish oil by using enzyme method |
| CN102260585A (en) * | 2011-06-28 | 2011-11-30 | 泰祥集团技术开发有限公司 | Method for extracting fish oil from squid viscera by using animal proteolytic enzyme |
| CN101181079B (en) * | 2007-12-19 | 2012-08-22 | 湘西自治州金凤凰农业生物科技有限公司 | Spot fork-tail catfish oil and preparation method thereof |
| CN102888274A (en) * | 2012-10-04 | 2013-01-23 | 宁波兰洋水产食品有限公司 | Method for extracting crude fish oil from hairtail silvery fat |
| CN103773590A (en) * | 2014-01-23 | 2014-05-07 | 吉首大学 | Method for extracting fish oil from internal organs of elopichthys bambusa by utilizing neutral protease |
| CN105296136A (en) * | 2015-09-30 | 2016-02-03 | 大连工业大学 | Method for extracting grease in gonad of sturgeon by utilizing aqueous enzymatic method |
| CN107320497A (en) * | 2017-07-24 | 2017-11-07 | 武汉轻工大学 | Purposes of the sturgeon extract oil in lowering fat and protecting liver health food or medicine is prepared |
| CN107400559A (en) * | 2017-08-10 | 2017-11-28 | 福建海富特生物科技股份有限公司 | A kind of method in semi continuous feed fish oil refinery practice addition antioxidant |
| JP2018520699A (en) * | 2015-06-16 | 2018-08-02 | アシペンサー バイオテック リミテッド | Oil derived from sturgeon egg sac membrane |
| CN110904166A (en) * | 2019-12-11 | 2020-03-24 | 付俊 | Method for extracting brain gold from giant salamander |
-
2006
- 2006-12-05 CN CN 200610124065 patent/CN1974741A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101181079B (en) * | 2007-12-19 | 2012-08-22 | 湘西自治州金凤凰农业生物科技有限公司 | Spot fork-tail catfish oil and preparation method thereof |
| CN101824364A (en) * | 2010-04-09 | 2010-09-08 | 浙江兴业集团有限公司 | Method for deacidifying and refining high-acid value fish oil by using enzyme method |
| CN101824364B (en) * | 2010-04-09 | 2012-08-22 | 浙江兴业集团有限公司 | Method for deacidifying and refining high-acid value fish oil by using enzyme method |
| CN102260585A (en) * | 2011-06-28 | 2011-11-30 | 泰祥集团技术开发有限公司 | Method for extracting fish oil from squid viscera by using animal proteolytic enzyme |
| CN102888274A (en) * | 2012-10-04 | 2013-01-23 | 宁波兰洋水产食品有限公司 | Method for extracting crude fish oil from hairtail silvery fat |
| CN103773590A (en) * | 2014-01-23 | 2014-05-07 | 吉首大学 | Method for extracting fish oil from internal organs of elopichthys bambusa by utilizing neutral protease |
| JP2018520699A (en) * | 2015-06-16 | 2018-08-02 | アシペンサー バイオテック リミテッド | Oil derived from sturgeon egg sac membrane |
| US11197490B2 (en) * | 2015-06-16 | 2021-12-14 | Shah Caviar Limited | Sturgeon roe sack membrane-derived oil |
| CN105296136A (en) * | 2015-09-30 | 2016-02-03 | 大连工业大学 | Method for extracting grease in gonad of sturgeon by utilizing aqueous enzymatic method |
| CN107320497A (en) * | 2017-07-24 | 2017-11-07 | 武汉轻工大学 | Purposes of the sturgeon extract oil in lowering fat and protecting liver health food or medicine is prepared |
| CN107400559A (en) * | 2017-08-10 | 2017-11-28 | 福建海富特生物科技股份有限公司 | A kind of method in semi continuous feed fish oil refinery practice addition antioxidant |
| CN110904166A (en) * | 2019-12-11 | 2020-03-24 | 付俊 | Method for extracting brain gold from giant salamander |
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