CN1970786A - Method for rapid detection of bacteria - Google Patents
Method for rapid detection of bacteria Download PDFInfo
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- CN1970786A CN1970786A CN 200510110646 CN200510110646A CN1970786A CN 1970786 A CN1970786 A CN 1970786A CN 200510110646 CN200510110646 CN 200510110646 CN 200510110646 A CN200510110646 A CN 200510110646A CN 1970786 A CN1970786 A CN 1970786A
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- bacterium
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Abstract
The invention discloses a rapid bacteria detecting method, which is characterized by the following: blending bacterial sample with phage solution; amplifying pressure noise of mixed solution to analyze; comparing noise character and reference character to obtain corresponding bacteria; blending the detected bacterial sample and mixed bacterial solution on the antenna; detecting whether the bacteria are infected; affirming the bacteria within 8-60min.
Description
Technical field
The invention belongs to field of biological detection, relate to the technology of rapid detection bacterium.
Background technology
The equipment of existing Bacteria Detection Technology Need costliness and long detection time, the most frequently used detection technique is that bacterium is separated and cultivation at present, uses microscopic examination behind the bacterium colony of single bacterium to be formed.This process need time more than one day, and in the cultivation of bacterium and sepn process, be subject to and pollute and then influence detects effect;
Another kind of commonly used detection technique is to use gene-amplificative instrament (PCR), and its detection speed is very fast relatively, but generally also needs more than several hours, and this detection method can not be distinguished dead and the bacterium that lives.
Summary of the invention
The purpose of this invention is to provide a kind of new Bacteria Detection technology, can within a short period of time detect, identify the kind of bacterium, and the discovery that detects, evaluation is the work bacterium.
For achieving the above object, solution of the present invention is: the method for a kind of rapid detection bacterium, comprise: the sample that will contain tested bacteria is with after known phage solution mixes, detect the voltage noise of this mixing solutions and, this characteristics of noise and reference feature are compared to detect whether contain the bacterium corresponding with known phage in this sample analyzing after its amplification.
Further, in the step that characteristics of noise is compared,,, can determine to contain in this sample the bacterium corresponding with known phage as infected by determining whether infected detection of bacterium.
Determine that at this whether bacterium is to obtain its power spectrum by voltage noise is sent into data-processing equipment after amplifying in the infected step, determines by the shape of power spectrum whether bacterium is infected.
Above-mentioned data-processing equipment comprises spectrum analyzer, computer.
Identical when demonstrating when comparing by data-processing equipment power spectrum that obtains and the standard power spectrum of measuring in advance that the infection reaction is arranged, can determine that bacterium is infected.
Determine that at this whether bacterium is to obtain waveform by voltage noise is sent into waveform generation equipment after amplifying in the infected step, whether infected by whether containing standard compliant broad pulse in the detection waveform to determine bacterium.
This waveform generation equipment comprises oscilloscope or data collecting card, computer.
It is infected to determine that bacterium whether in the infected step, a standard compliant broad pulse occurs in voltage noise, can think that a bacterium is arranged whether to contain standard compliant broad pulse in detection waveform, thereby determines that bacterium and used phage mate.
A kind of device that can realize the method for above-mentioned rapid detection bacterium comprises: detect antenna, comprise chip base; it is provided with metallic film; be extended with the tip on this metallic film, the part beyond its upper prong is separated by the protective layer and the external world, and metallic film is connected with amplifier by lead.
Further, also comprise: spectrum analyzer, it is connected with amplifier by lead.
Perhaps also comprise: data collecting card, the one end is connected with amplifier by lead, and the other end connects data-processing equipment.
The principle of such scheme is, when bacterium during by phage-infect, phage can make a call to a hole on the cytolemma of bacterium, the DNA of oneself is injected bacterium, DNA copies tens filial generation phages in bacterium, at last, bacterial cell breaks, and progeny phage discharges, inject the process of bacterium at phage DNA, because the ionic concn in the bacterial cell is higher than the external world, intracellular ion can discharge, and (sees also document: 1.P.Boulanger, and L.Letellier, J Biol Chem vol.263, pp.9767,1988; 2.L.Letellier, and P.Boulanger, Biochimie vol.71, pp.167,1989), thus the current potential around changing; Potential variation in this little scope can be detected, and shows as the voltage noise in the detection means.By spectrum analysis or wave form analysis to voltage noise, can determine that whether bacterium is by phage-infect, utilize the specificity of phage-infect bacterium, be that a kind of phage only infects a kind of or a bacterioid, under the prerequisite of known phage, promptly know the type of bacterium,, use technology of the present invention can in 8-60 minute, detect, identify the bacterium in the sample because phage was finished in 8-60 minute usually to the infection of bacterium.
Description of drawings
Fig. 1 is the systematic schematic diagram of an embodiment of the present invention;
Fig. 2 is that the system of another kind of embodiment of the present invention realizes schematic diagram;
Fig. 3 is the vertical section structure synoptic diagram of the detection antenna of an embodiment of the present invention;
Fig. 4 is the cross section structure synoptic diagram of the detection antenna of an embodiment of the present invention; Be clear meter, the protective layer 10 on it is not drawn.
Fig. 5 has shown a kind of embodiment that uses the present invention to detect: intestinal bacteria solution respectively with detect after coliphage and tubercule bacillus phage mix after frequency-power spectrum density synoptic diagram of obtaining.
Embodiment
See also Fig. 1-5, embodiments of the invention, detecting antenna can be by plating or evaporation, and the two block metallic films 9 of modes such as deposit on chip base 8 form, and these two blocks of metallic films 9 respectively stretch out a tip.Metallic film 9 beyond most advanced and sophisticated is separated with the external world by protective layer 10, has only two tips can contact liquid or the gas that adds.Metallic film 9 keeps electrical interconnection by lead and amplifier 4.
This metallic film is stable, the corrosion resistant type of chemical property, titanium for example, chromium, gold, platinum etc.; Use monolithic or polylith also can, but be good with two.
When bacterial detection, the solution that will contain tested bacteria 1 with drip on two tips of metallic film 9 after the solution that contains known phage 2 mixes, voltage between two metallic films 9 is exaggerated device 4 and amplifies the back or send into spectrum analyzer 5 and analyze, or sends into computer 7 through data collecting card 6 and analyze.
If bacterium 1 can be infected by phage 2, then bacterium 1 can discharge ion, at this moment the power spectrum of the voltage of 9 of metallic films and the power spectrum shape that not have infection, both power spectrum density was with the distribution of frequency, different fully, there is the composition that infects with the kind of the power spectrum that not have infection and bacterium and solution relevant, for example, tubercule bacillus is not white Gaussian noise in the 1-10Hz scope by the power spectrum of phage-infect, and infected power spectrum is 1/f noise in 1-10Hz, and to the other bacterial solution, the power spectrum that does not have to infect is 1/f noise in 1-10Hz, and the power spectrum of infection is 1/f in 1-10Hz
2Noise.Infected or the not infected power spectrum of various bacteriums can be measured by method described in the invention in advance.
Fig. 5 has shown that intestinal bacteria solution detects the frequency-power spectrum density synoptic diagram that obtains after detecting on the antenna 3 with dripping to after coliphage and tubercule bacillus phage mix respectively, after coliphage mixes, the phage-infect intestinal bacteria, voltage noise power spectrum in the detected detection antenna is a 1/f noise, but after the tubercule bacillus phage mixes, phage can not ehec infection, and the voltage noise power spectrum is a white Gaussian noise.
To phage whether bacterial infection determine also can be by making the analysis that detects the voltage noise waveform in the antenna, a standard compliant broad pulse appears in voltage noise, can think that a bacterium is arranged is infected, thereby determine that bacterium and used phage mate." standard compliant broad pulse " is meant: pulse height surpasses 3 times of noise average powers or higher, and one long period of pulse persistance, as more than 0.05 second, concrete height and time length are by bacterial species and solution composition decision.Infected pulse height that causes of various bacteriums and time length can be measured by method described in the invention in advance.
The above-mentioned description to embodiment is can understand and apply the invention for ease of those skilled in the art.The person skilled in the art obviously can easily make various modifications to these embodiment, and needn't pass through performing creative labour being applied in the General Principle of this explanation among other embodiment.Therefore, the invention is not restricted to the embodiment here, those skilled in the art should be within protection scope of the present invention for improvement and modification that the present invention makes according to announcement of the present invention.
Claims (10)
1, the method for a kind of rapid detection bacterium, it is characterized in that: comprising: the sample that will contain tested bacteria is with after known phage solution mixes, detect the voltage noise of this mixing solutions and, this characteristics of noise and reference feature are compared to detect whether contain the bacterium corresponding with known phage in this sample analyzing after its amplification.
2, the method for rapid detection bacterium according to claim 1, it is characterized in that: in the step that characteristics of noise is compared by determining whether infected detection of bacterium, as infected, can determine to contain in this sample the bacterium corresponding with known phage.
3, the method for rapid detection bacterium according to claim 2, it is characterized in that: determine that at this whether bacterium is to obtain its power spectrum by voltage noise is sent into data-processing equipment after amplifying in the infected step, determines by the shape of power spectrum whether bacterium is infected.
4, the method for rapid detection bacterium according to claim 3 is characterized in that: demonstrate identically when the power spectrum that will obtain by data-processing equipment and the standard power spectrum that infection is arranged measured in advance compare, can determine that bacterium is infected.
5, the method for rapid detection bacterium according to claim 2, it is characterized in that: determine that at this whether bacterium is to obtain waveform by voltage noise is sent into waveform generation equipment after amplifying in the infected step, whether infected by whether containing standard compliant broad pulse in the detection waveform to determine bacterium.
6, the method for rapid detection bacterium according to claim 5, it is characterized in that: in detection waveform, whether contain standard compliant broad pulse to determine that bacterium is whether in the infected step, a standard compliant broad pulse appears in voltage noise, can think that a bacterium is arranged is infected, thereby determine that bacterium and used phage mate.
7, a kind of device of realizing the method for the described rapid detection bacterium of claim 1; it is characterized in that: comprising: detect antenna; comprise chip base; it is provided with metallic film; be extended with the tip on this metallic film; part beyond its upper prong is separated by the protective layer and the external world, is connected with amplifier by lead.
8, the device of rapid detection bacterium according to claim 7 is characterized in that: also comprise: spectrum analyzer, it is connected with amplifier by lead.
9, the device of rapid detection bacterium according to claim 7 is characterized in that: also comprise: data collecting card, and the one end is connected with amplifier by lead, and the other end connects data-processing equipment.
10, the device of rapid detection bacterium according to claim 7 is characterized in that: this metallic film is that chemical property is stable, corrosion resistant type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510110646 CN1970786A (en) | 2005-11-23 | 2005-11-23 | Method for rapid detection of bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510110646 CN1970786A (en) | 2005-11-23 | 2005-11-23 | Method for rapid detection of bacteria |
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|---|---|
| CN1970786A true CN1970786A (en) | 2007-05-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200510110646 Pending CN1970786A (en) | 2005-11-23 | 2005-11-23 | Method for rapid detection of bacteria |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107890577A (en) * | 2017-10-23 | 2018-04-10 | 南方科技大学 | One kind sterilizing robot system |
| CN108474792A (en) * | 2015-11-20 | 2018-08-31 | 辛纳米拉股份公司 | Method and apparatus for detection bacterium |
-
2005
- 2005-11-23 CN CN 200510110646 patent/CN1970786A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108474792A (en) * | 2015-11-20 | 2018-08-31 | 辛纳米拉股份公司 | Method and apparatus for detection bacterium |
| CN108474792B (en) * | 2015-11-20 | 2021-02-02 | 辛纳米拉股份公司 | Method and apparatus for detecting bacteria |
| CN107890577A (en) * | 2017-10-23 | 2018-04-10 | 南方科技大学 | One kind sterilizing robot system |
| CN107890577B (en) * | 2017-10-23 | 2021-04-06 | 南方科技大学 | Sterilization robot system |
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