CN1970762A - 人类肝脏相关基因 - Google Patents
人类肝脏相关基因 Download PDFInfo
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- CN1970762A CN1970762A CNA2005101105976A CN200510110597A CN1970762A CN 1970762 A CN1970762 A CN 1970762A CN A2005101105976 A CNA2005101105976 A CN A2005101105976A CN 200510110597 A CN200510110597 A CN 200510110597A CN 1970762 A CN1970762 A CN 1970762A
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Abstract
本发明公开了一类在人类肝脏中表达的相关基因序列,它包括:SEQ ID No.1~SEQ ID No.40所示的序列;SEQ ID No.1~SEQ ID No.40所示的序列中每条序列的互补序列;与SEQ ID No.1~SEQ ID No.40所示的序列中每条序列有至少70%同源性的序列;上述三种序列中一条或数条的组合。利用本发明的人类肝脏相关基因,可为研究肝脏疾病的致病机理以及开发治疗肝脏疾病的药物发挥重要作用。
Description
技术领域
本发明涉及生物技术领域,尤其涉及一类在人类肝脏中表达的相关基因。
背景技术
肝脏是人体的第一大器官,是物质代谢、能量转换及供应的“枢纽”,是机体内多种重要信息调控分子的“集散地”,是机体内再生能力最强的器官,在人类生命活动中占有重要地位。肝脏的主要功能是进行糖的分解、贮存糖原;参与蛋白质、脂肪、维生素、激素的代谢;解毒;分泌胆汁;吞噬、防御机能;制造凝血因子;调节血容量及水电解质平衡;产生热量等。在胚胎时期肝脏还有造血功能。
肝脏是淋巴细胞以外最常见的病原体持续感染的场所,肝脏疾病又分为肝炎、肝硬化、脂肪肝、肝癌等。现代医学实验证明,肝病病毒侵入人体后,并不直接引起肝细胞的损害,只是在肝细胞内吸收营养赖以生存,并在肝细胞内复制、繁殖。其复制病毒的“零部件”如表面抗原(HBsAg)、e抗原(HBeAg)释放在肝细胞膜上,引起人体免疫系统对这些抗原物质产生免疫反应,这种反应造成肝细胞的损伤、坏死。免疫反应的强弱决定于肝脏受损程度及临床症状轻重。这场由病毒引发的、免疫系统对肝细胞的战争,使大约25%的患者的肝脏成为战火连绵的战场,肝脏的损伤由此加重。肝病的危害绝不仅仅限于肝脏本身,它还可以引起其它多种疾病。常见的有:(1)糖尿病;(2)胰腺炎;(3)胆道感染;(4)功能性肾衰竭;(5)胆汗性肾病;(6)肾小球肾炎;(7)肾小管酸中毒;(8)溶血性贫血;(9)再生障碍性贫血;(10)心肌炎和心包炎;(11)结节性动脉炎;(12)消化性溃疡;(13)自发性腹膜炎;(14)性激素代谢紊乱;(15)甲状腺功能改变;(16)肝性骨病,等等。肝病不仅对患者的身体甚至生命造成危害,而且对患者心理上的打击也是十分沉重的。无论是肝病患者还是病毒携带者,在生活、社交、求职、升学等方面都会受到严重影响。
肝脏疾病相关基因在以肝炎、肝癌为代表的重大肝病的控制、诊断、防治与新药研制领域起着重要的作用。
在本发明之前,还没有出现涉及本发明的一类人类肝脏相关基因的公开报道。
发明内容
本发明要解决的技术问题是提供一类在人类肝脏中表达的相关基因。
为了解决上述技术问题,本发明通过如下技术方案实现:
在本发明的一个方面,提供了一类在人类肝脏中表达的相关基因,其包括:
(a)SEQ ID No.1~SEQ ID No.40所示的序列;
(b)SEQ ID No.1~SEQ ID No.40所示的序列中每条序列的互补序列;
(c)与SEQ ID No.1~SEQ ID No.40所示的序列中每条序列有至少70%同源性的序列;
(d)上述(a)~(c)中一条或数条的组合。
较佳地,所述序列包括具有SEQ ID No.1~SEQ ID No.40所示的序列。
本发明还提供了一种探针分子,所述的探针分子含有上述序列中约8-100个连续的核苷酸。
由本发明的一类在人类肝脏中表达的相关基因,可为研究肝脏疾病的致病机理以及开发治疗肝脏疾病的药物发挥重要作用。
具体实施方式
下面结合具体实施例,进一步阐述本发明。应理解,这些实施例仅用于说明本发明而不是限制本发明的范围。下列实施例中未注明具体条件的实验方法,通常按照常规条件如Sambrook等人,分子克隆:实验室手册(New York:Cold Spring HarborLaboratory Press,1989)中所述的条件,或按照制造厂商所建议的条件。
实施例1
人肝脏组织的mRNA的分离
组织分离(Tissue isolation):肝脏来源于5个成年男性,在肝脏切除手术后,将肝脏组织立即置于液氮中冷冻保存。
mRNA的分离(mRNA isolation):取出肝脏组织,用研钵研碎,加入盛有裂解液的50ml管,充分振荡后,再移入玻璃匀浆器内,匀浆后移至50ml新管,抽提总RNA(TRIzol Reagents,Gibco,NY,USA)。用甲醛变性胶电泳鉴定总RNA质量。用带Oligod(T)的纤维素柱分离总RNA中的mRNA,定量。
实施例2
cDNA文库的构建(Constuction of cDNA library)
以mRNA为模板,合成双链cDNA。补平末端后,加含EcoRI切点的接头。磷酸化EcoRI末端后,用XhoI限制性内切酶消化1.5小时,再进行片断分离。过柱筛选长度>500bp的片段,用酚-氯仿抽提,乙醇沉淀,无菌水溶解,连接至Uni-ZAP XR载体(Strategene,CA9203,USA),以ZAP-cDNA Gigapack III Gold Cloning Kit(Strategene,CA9203,USA)进行包装,宿主菌使用XL 1 Blue MRF’(Strategene,CA9203,USA)细菌。涂板并测定滴度。
实施例3
测序及数据库建立(Seqencing and Database Constructing)
挑选文库中有外源片段插入的克隆,扩增后抽提质粒(Qiagen Germany),用T3和T7作为3’和5’端的通用引物,采用终止物荧光标记(Big-Dye,Perkin-Elmer,USA)的方法,在ABI 377测序仪(Perkin-Elmer,USA)上进行EST大规模测序。测序结果用FACTURA软件去除载体序列,传输到SUN Ultra 450 Server上进行下一步的处理。所有的序列信息再用GCG软件包(Wisconsin group,USA)中的BLAST和FASTA软件搜索已有的数据库(Genebank+EMBL),将无同源性或同源性低于95%的序列视为新基因建立数据库。
实施例4
基因的全长克隆(Cloning of Full-length cDNA)
在得到的新基因片段序列信息基础上,进行cDNA全长克隆,分两阶段进行:
(1)“电子克隆”(Electronic Cloning)
以新基因片段序列作为探针搜寻dbEST数据库,将重叠序列>50bp,同源性在98%以上的表达序列标签(Expressed Sequence Tag,简称“EST”)序列认为同一序列(Consensus Sequence),取出并用AUTOASSEMBLER软件进行连接,部分EST可以延伸探针序列。再用STRIDER软件分析被延伸的序列是否具有完整的开放阅读框架(OpenReading Frame,ORF),用BLAST搜寻Genbank或SwissProt以确定该序列的核苷酸和氨基酸水平上是否与其他物种有同源性,以帮助判别所得到的基因全长完整性如何。通过电子克隆的方法,通常可获取人肝脏相关基因的全长序列。
(2)cDNA末端快速扩增(Rapid Amplification of cDNA Ends,RACE)
如果通过“电子克隆”方法仍未得到完整的cDNA全长,则在已有序列5’或3’端设计引物,在人类肝脏Marathon-Ready cDNA文库(Clontech Lab,Inc,USA)中进行长距离PCR反应。然后对PCR产物克隆、测序。用AUTOASSEMBLER及STRIDER软件分析被延长的序列有无完整的ORF,如无,重复上述过程直至获得全长。
(3)RT-PCR
对于5’和3’端的已知的序列,如果中间有一段间隙(gap)无法从已有的公共数据库或自身数据库获得,可考虑采用RT-PCR的方法。在序列5’端设计引物,3’端引物采用Oligo-dT,在肝脏总RNA库中进行扩增。然后对产物进行克隆、测序。最后拼接便获得全长。
通过组合使用上述3种方法,可获得人肝脏相关蛋白的全长编码序列。
序列表
<110>上海人类基因组研究中心
<120>人类肝脏相关基因
<130>NP-10159
<160>40
<210>1
<211>969
<212>DNA
<213>Homo sapiens
<400>1
1 cttactttat ttgcatctca ncaaaggttc tcatgtggaa cctgattggc atcaaaccaa
61 agttcgtagg ccaacaaaga tgggccactc acaagcttcc catttgtaga tctcagtgcc
121 tatgagtatc tgacacctgt tcctctcttc agtctcttag ggaggcttaa atctgtctca
181 ggtgtgctaa gagtgccagc ccaaggtggt caaaagtcca caaaactgca gtctttgctg
241 ggatagtaag ccaagcagtg cctggacagc agagttcttt tcttgggcaa cagataacca
301 gacaggactc taatcgtgct cttattcaac attcttctgt ctctgcctag actggaataa
361 aaagccaatc tctctcgtgg cacagggaag gagatacaag ctcgtttaca tgtgatagat
421 ctaacaaagg catctaccga agtctggtct ggatagacgg cacagggagc tcttaggtca
481 gcgctgctgg ttggaggaca ttcctgagtc cagctttgca gcctttgtgc aacagtactt
541 tcccaggatc cacaggaaat actcagagtc cacctggaca ttttacttat attcagtttc
601 caagtgtcag aggggttcag gactgtctgg cctggggggc ctcctcatga cctcaattca
661 ggattatcta ctcaaagcat taaacaaaag gaaatacatt ttcaggatga aggctgctct
721 tttaagccct gctgaagarg catttcaaac aggattggaa tagggaaacc ggcactcagc
781 tcggcgcaag ggcgggtgcc ttcagactag agagcctctc ctccggtgcg ctgcaagtag
841 ggcctcggct cgaggtcaac attctagttg tccagcgctc cctctccggc acctcggtga
901 ggctagttga cccgacaggc gcggatcatg agcagctgca ggagaatgaa gagcggggac
961 gtaatgagg
<210>2
<211>1162
<212>DNA
<213>Homo sapiens
<400>2
1 ggnsggctgt gtggtctggt gtttgggagg gaactccacc cccaccaggc caaccatgga
61 gctagaaaca gagacagcag ggagggcaaa gctggccact gcctgctcca ccccttcaca
121 gcccagagca gaacagggtc tgctctactc tcaaggtgag tgacagagag ccggtactgt
181 ttctgcccct ggcataccct gagaacccat gtgacttctg ttgtgctcag cccctgtgcc
241 cttcctgggc ctgatccaca tgtgtcaaca cacacactcc ctctcacagt ctccaaacag
301 cactgcagag cctagctgca tctgccaggt tcaaagagga atttttcaca tttgctcact
361 tccaatctcc atcttccttc ctctgtctcc cactctccca ctctcagtag ccgcatccca
421 gccctgccat actcccttct cagggacagg agactcagtg ggcagctggc ctcagctctc
481 ctaacaggaa aaaaacctgt acagcattag tgccagggct cctgccctcc caagcgctga
541 gcccagaaat ttggacaaat gagctgcctc ttaactgcaa aaaacaattt taaaaaagca
601 aaagatcaaa caaacagacc aaaaagcata aataaacagc agctgggcca gcgaggagga
661 aggcagggtg accctcagtg gctccctgtg cccatctcag cctcttgcca taaaactcag
721 ccatcagtgg ccaggatgac agcagttccg aagatgccca cactctctcc aaggagcttc
781 atctggttcc agaactcaac acgccgcgtg ttgtgccagt kagcaacatt gccatcaatg
841 agcagcatga cagggggcag cagtwcagcc aggatctggg cagcgagggt cacgtagtag
901 ccttgacaga aaggccaggg ccaggatgcc ataaagcacg gagaacagct ggatcatcag
961 ctccctcctt gggagatggk tcagatacgc cagcgggtct ccttgctgtc tgcagtgagt
1021 gggcaaacag atgaggtaga tacccaggaa cactgggcgg gggrctgcag gagsggctgc
1081 agggttycgg cggtamgctc ccagcaccgc gggcaacaaa gaaagcgcgg atgwkgsaat
1141 gttcgcrrtt mataggactt ag
<210>3
<211>1125
<212>DNA
<213>Homo sapiens
<400>3
1 ccatgtttaa aattttgaag aaatccctgg agtagacaat ccctagagca ctgacatgct
61 gtggtccaag gttcaccagg aacagtgcct gtgccctgcc acatggcggg gttccttcaa
121 gcctctggtg atgaaggtgc tcagggaaag gaggacggag gggcgcggaa ggacccagcc
181 actgctggcc accctggcct ctgctctgtg ttcacggggg agcagactct ggggaggcgc
241 ccctcctcac cacggcctgc aggcgggctg gagcctgcgc cttcaggggc cttgcatctc
301 cagctggacc ccctggcaca gatgtgctgc agtagcccgt tgaccacatc cagagtctcg
361 tccttctcca gcacgatgtc ataggagttc atgtagcgct cccgccgctc ctccaccttg
421 tcattcagga agccaatttt gagaatgttc tgcacaccag gaaccccatc ggccatggtg
481 aggtccccga tagagtctcc cagcaggatg acattggttt tgccctcaag ttgctggaag
541 taaccacagt tctcacacac agagctgttc ttgttgtatg tgtgtatcag ctggccctta
601 aatccctgga gaaaaccctg agcattgcat tggactctct aaccacctgg gctatctgaa
661 acttctgaat cttctgctga cataggagat tgtgcgcttt ggtccaccat tccaccatat
721 gaggtagctt ctccttgacg gtccggtgtg ggtcgatctc aattgggtaa tagtggtgaa
781 ggagcgctgt gagctctttc cgacactyct cactgatgat cttgctatta tccagaatat
841 tgtwagaaga agggcatcgc tttccattat atgcaaacct gctcaaggtc atgtcaaaat
901 cagaaatcac ctgttaccgg tctccgccgc ccttgcggag ggcgcccacg atctcctgcm
961 ccgcccaggc tgccgcatca ggaccgtggc yttcatcagg gtgctcactt tsccgagacc
1021 gagaraacam tkaccctgcs camgctgggc csgaggggrc cgggggycgy cggggcscgt
1081 ttaagggggg cggggkggca ggggsgctmt gcctggtgaa sgcgg
<210>4
<211>842
<212>DNA
<213>Homo sapiens
<400>4
1 tttaatatca taaatcsgtg tggggccaca cctctcactg ccaggcccgt gggctcctat
61 ctccatattg cctggsagst gcctgcsgcc aggscttccc ccatccccta ggaaggatcc
121 agcaccagcc tgagavggga ggcaacagca gccaaagggg tgggaggvgg aggagacaga
181 agcatgtscb gcaggcgcat gtgtgtttgt tcttacatgt gtgaacacat ataaagtgtc
241 aggtttacag accctggctc aaggacagty taggatggga aaggaggtag ggcgagaaga
301 agcacatatt ttctccctgg tgcttcagcc tcaccctatc caagggacag acatatgggg
361 tgtgagamac ccatccccag gtcccagcct tcaggactgg agtccttttg agtctggtgg
421 agtcacagac ccagtctttg ggggacactg ggtctgtctc cttttgaaag ccctggaaag
481 gygggaggta agaagtaaag ggagaaaggc ccctgctaga agaacttgac gcctcggcca
541 tcgctgacag taatgatctc ggccttgtgc tcctgctgtc gggcctgcag agcccgcagt
601 agagtggctt catccagccc gtggaactcc tcaccctctg tgtctccccc attagtcagt
661 ccatacaggg taaagacgga gtggttctgg ccactcctgg aaacccactg atagatgagt
721 ttcccccatt cttctggcct ccgccacatg atcaggaagc tggacttgct cttatccaac
781 cactcgaggt tccctttctt cctcagttcc tctaatacaa tctggatcga ctccacagga
841 ag
<210>5
<211>854
<212>DNA
<213>Homo sapiens
<400>5
1 cgcacaccga gagccacttc cggaacaagc gtcgcgtntt ctgaggagaa actcttggtg
61 agaattccca gagtgataat ggctacctac agcctggcga acgagagact acgcgctctg
121 gaagacattg aacgggaaat cggcgccatc cttcagaatg caggtactgt gatcctagaa
181 ttgtccaagg aaaaaactaa cgagcggctc ctagaccggc aggcggcggc cttcaccgct
241 tcagtgcaac acgtggaggc ggagctgtca gctcagatcc gctacctcac ccaggtggcc
301 acagggcagc cccatgaggg ctccagctac tcttcgagga aggactgtca gatggctctg
361 aagcgagtgg actatgcccg cctcaagctc agtgatgtgg ctcgaacctg tgagcagatg
421 ctggagaact aggccaggga gatggaccca gagctgagag ggagaccatc acctgtgcca
481 tgggacagaa cctgggaaca tgtagggtgg ggagtatgag caccaacata ccctgctggt
541 caaagtaccc taggacaaag gggcaaatgg tgggcatgga aaaactgaag cccgagcctg
601 cccacagcta tgccttggcc tctccagcag ggtgctcctg ctctgcactc cagaaacacc
661 ctgacaggct cagagaggag atggaaactg acatacccaa cccacttcct caaacacttc
721 atgctgctgc cccagccttc ccgcagactt ggcctaatga aaatacatac ttcttcattc
781 ggagagacaa aacaagaact agagttttaa tgataataaa agcaataata attaaagcat
841 taccataaaa aaaa
<210>6
<211>798
<212>DNA
<213>Homo sapiens
<400>6
1 tataatggta actgtaaaat gtactatttt taatgggtgt gcatgtcaag mttttcttta
61 gaaatacact ggtctggtct aatttattta agcaggagca ctttaaagta tcccacccta
121 ccccattcca cccccagtgg acagaaagga aattgactga cttgagrgga tgcagacatc
181 tgggttattc caacagacca gtggttagga ggagggggtg ggtagcatta tggcctcggg
241 caggcccccc caccctgagc ctctgaaagc tgactttatc tgtaagaggg aggtcaggct
301 cgccttctca atancgtgta tttggataar atnagtttct tctggantac acttaggcan
361 ancanggctn gcttanggct nnancctcag cgtttcacaa cttctcacct tctcttccat
421 gtcagtcana atggcatcca actttgccac cacctnccgt gcattgtcca nnctnaanca
481 cattgggggc ttaaacttca agatnttcct cccagggcca tcagtgctca gcaaaacgta
541 ttctccttca gccttgatac caagtaggca gcctcttcag ttgctggtgt ccttgtggcc
601 tcatctttga tcagatccac accaatgaag agcccaacac ccckgacatc cccgacgatg
661 ggatgtttga ttttttgctg cccgaggagc tgcatcagga agctgcctac actggtggca
721 tgatcctgga gctgctcctt ctccaagaca ttcaggacgg ccatccccac agcgcaggac
781 actgggctgc ccccaaac
<210>7
<211>1162
<212>DNA
<213>Homo sapiens
<400>7
1 aatataagca tttaatgtca aagaaataaa ggtaatttta caaactcagt ttttgtaagt
61 acatgaagtt tctatttgat tatgtggttt tatatcacat tcgttcaaat gcatttctct
121 cccttagagg gactattcca acatcaatmc tttggaatta tttcagtcat ccttaacatg
181 tgactttacc aaagaccttg aagctaaaca aacaagcaaa acaaaatttc aatgaatctt
241 agatgaatgg aataagaaat agtmatcaca tgtaaattag ggatgttcat ctccaaccaa
301 gaaactgtca aaatgtttct tctgatacag cagttataag tcagagcctt caaaaaamaa
361 gggcagaaca agagtacaat aaaagaagca tctgcaactt aagcmtccca cagtcctaag
421 cctgatatgc gcaaagaaaa gcctctttcc cgcaaactaa attccatcca tttgagcttt
481 cagagatcga tgcccaagag ctatcattaa tatatacagc atattgactc tagttgcatc
541 aattatgcct gaagagttta atacccatcc aagtccaaag gtggaagaat acatacactg
601 gtatggtaat aggcaagagc aggctgtaaa agcaaaggct ggctgtgcta gtgcagccct
661 gtgggaagtt ttcttccaca gaggctgagt agaacagtcc tgctaaagaa accagtggaa
721 taagaacagt caacgtagga agagacagaa acattcttct cttccactta ttacctgcca
781 ctgtgtttta aaaggtatat ggtattaaga aaagttggct gttgcgtttt tttaattttt
841 ttaaattatg aactagcgct gctccacttc ttcttgcttc ttactttgac gtgtcatcct
901 agctgcctgt ggtatcatat tagggatccc atcaatgatt ggataagcta ttccaactct
961 tcattaatca attcgtttgt tgatgcttca tatctgagcg gcttcttkga ggagcgggca
1021 cacaaggaac tccagcagcg csggrtcgaa gtcgcggggg ggttctcagt yttyttgccc
1081 gggcggccaa aggsggaccc saagcgtgsa ggsactamgg gggacgsggr gggggggggg
1141 gggcccgcag gstgggggga gc
<210>8
<211>908
<212>DNA
<213>Homo sapiens
<400>8
1 ggtgtttcat ttntngtagc ccagnnatgg nagtgcaatg gtgcgatcwt agctcactgc
61 aacctccacc tcccaggttc aagcagttct tctgcttcms cctcctgagt ygctgggatt
121 acagatgtyc gcsaccatgc ccagctaatt ttgtttgtat ttttagtaga gacggggktt
181 catcgtgttg gscagggtgg tctcaaactc ctaacctcag gkgatctgct cacctcgggc
241 tcccaaagkg ctgggattat tatgggcata agccactgtg cccagcccaa aaaggsattt
301 taaacaaaaa ttctgacgtt taaaaactga aaatatatcg cgccactgca ctactgcctg
361 ggcaacagag caagamtctg tctcaaaaaa acaaaacaaa aaacaaaaaa ccctgaaaat
421 atgagcccag gagttcgagg ctgcaatgag ccaatactgc acctttgtgc ttctgcctgg
481 rcgacagagt gagaccttgt cgataagaaa aaaaaaaacc tgaaaacaga aaagagggct
541 tttacaatgt tgatgaactc atcttagagt gcatgattta taaatacata aatatccatg
601 catactttta cagtgataac tcctgggaaa atgagaatgt taaaaaagcc aatctagaca
661 gagtccttct cattccacct gcctctttat gttggtaata ccaggagtct taagagagct
721 gctgtggtct atgtcacatt catcccagcc tcctttctaa tcctgtagct gatgatgaaa
781 atgtgataaa tcttctggct tagctttgat gctttttcat taattcactt ctggtagtaa
841 attgtggcag gyatyaaatt maatcamatm ggcaatttma ataamattgt ataaaagsya
901 tttattgt
<210>9
<211>1070
<212>DNA
<213>Homo sapiens
<400>9
1 attaatctat ttaatttttt tttttcagtt tgtatttttt attttacaat aaaaagtcaa
61 aaaaaataag caaaaagata aactggaaaa taagayttac atctcatata tatggacaaa
121 ggaccaatta cctccaaaca taaacagctc ctagaaatta ctgcaaagat caacaaccca
181 gtagaaaaat gaatgaagtt cccagaacag aaaacacaag tggcccttca aaaaaatgaa
241 gagaggctca gcctcttatg gtaagacaaa gagacaggat tttaaaaacc taggcctctt
301 cctagagttc ccttaaatat ctaggccaga tcatttttac ttcctggctt agaccctgcc
361 aagggctgcc cagctactca ggtgtttgtg tacttgtgga ctcaagtyat attgtcctga
421 tcttctggct gagtacggtt ttctcctcca gcaaagacaa tttggaggat gtactaagca
481 tgaagcgcta cttcctggcc cccatctctc ttcgcacagt gttccatcat ccagccatga
541 aagcacagct gagtgatcca agaggcagtt ccaattgttg actaacgtgt acctgcctat
601 gtgagtgtgt tctatgggaa ctcaggcctt agaatggttt caaagtagtg gctttcaaaa
661 ttactgtttg cctcttcaaa cttcacacct aaggaaaatg gaaacatgca gagcagggac
721 acagaagggg cattagctgg cgtggggtag gggcaagagc taattgtgaa ggaagaaggc
781 ctgagatcac gtagccatgt cggagaacag ctgtgctcgc tgccctgcct ctttgcgcgc
841 atgttaggca gccccaggct ccagctgctt gagtttctct tggagtgccc ggagctggct
901 tcgaccccag ttaatgcggt tctggaaaaa aaaaawatwt rcggscagct gcaggscatc
961 ccggaagctt gcttgagctt gaggtgactg ttaggactwg ggttccaacc mgttcagggg
1021 gtctgagggg ctaaaagrct csgktctggg gttaatgggc cytkccctcc
<210>10
<211>978
<212>DNA
<213>Homo sapiens
<400>10
1 tgctacttgt ctgagtnaaa ggccgnnaca ttcggttgac tggggaagtc cttctgcatt
61 aattacaata agtaatactc attgaaaact ttccttattt acacacagtt gaacagttat
121 tctaagaaac cattttccca ccctatttaa tattgacaga tttcttcagt tatnnacaaa
181 aactcnnnac ncncgctwtc ctwaakwact cwtctwtkty ccccttatyt cccctcatct
241 twtkttgtyc tctwwataca gggyttgctg ccctttcttc ttcttgcctt ttyagcagwt
301 gtkttaaggt tgcagaggtg gcagttaacc atagagtyac agaktttgat ttttttccat
361 ccwtcaggtc gtagaagytg ccactgagcc tkwttaagak ttggtactcw aaaatgcagw
421 ttkaattagt ttgaatgagg tgwttaaggw ttawtgaktw ktggttwagg aaacaaagwc
481 cattwgtggt tggggtwatw ttgctgtttt actgttggaa gcwggtttct ctaawwwaac
541 tttctgctgc cwtttacttw catacatttt aaagagttac tacaagattt tttaaaaatt
601 taaacagtta ttacaagtta atatatgtag tgtattgttc atgacactaa actgaactgt
661 tcatgacaca aagtatctta gaaaacaaat ctcggctggg ccagcacttt gtgaggtcaa
721 ggccagcaga tgacttgagg tcaggatttt gagaccagcc tggccaacat ggagaaacca
781 catctctact aaaaatacaa aaaaattagc caggcatggt ggckggkgcc tgthatccca
841 gctactgggg aggctgaggc aggagaatcg cttgaaccca ggaagtggtg gttgcagtwa
901 ggtgagatca taccactgca ctccagcctg ggcaacagag caatattwca tctcaaaaac
961 aaaaacaaaa accctagg
<210>11
<211>571
<212>DNA
<213>Homo sapiens
<400>11
1 gccttgtgat tcaccttact ctcggccatt tcctgagcca cgtctgaaga aatcctgtac
61 tgccaagata tatcctcaga caaagaaatg tccattagtc actcactaaa agggtaaaaa
121 gcaatcgaaa tgttttccta tctgaacccc tggtgccagc acagtataca gtagacaata
181 tatgccataa tttattgcct gaactcccca ttgatgtgac catgatatgt caatggaaag
241 tgagttcata aaaaataaat tctcattctc ttcaaataaa tttattgaga ttctatgaca
301 ctcccagaac gtggatatag caaacaacac tatgtaccat ccatgaaaaa acttacaatc
361 cagtaggaga aaaagacaag taaacaagca gttattgtag agcataataa gaaataaatg
421 aaaactgcca tatgggcatt ttagttggta cagtcaatgc caaatagaga gagcagtanc
481 agtttcctga aaaagtgatg gctanggtga gacctaaatg ccggtaggaa ttnaactggg
541 gnaaatgttg gaagcngcnc actttangga c
<210>12
<211>639
<212>DNA
<213>Homo sapiens
<400>12
1 atttgtagcc tgtggcaacc gtacccatct gtatccccaa gttctacacg gaatccctcc
61 agcacttcac gactccatta tccagctacc actaattcct ttgtgggcag agtccagcca
121 cgggcagccc ctgccctgcc tctggtcagg acaacacctg cccaaggaga gcaagggcct
181 gtgacctctg ctggcctgtg cgccctcacc tgtcacccag ctcagccctg tgtctggcct
241 acccagggag gaaggaacca gatttatcag cctgaaagga ggtagaagtt ttggagaaaa
301 gacactgttc gggtgtcaag taatgaactg actttattcg aacctcatct ccaatccaat
361 cccaccgtgg tctctctctc cctcagcttt tctggggcgg gttgactcgt gtgtcacttt
421 atcacagggg cacatggggt gagaagagga accttggcag taggagatac ctttgtacca
481 ggttntaatt tccagtgtac atctcagtcc cctcccctaa ccctcaacac agcctgggga
541 aagcccagct tgcaacacca gttctcagcc cagactgaca gccagataga tcttggccct
601 gagttnngcc aataaagatg ccgtgattat tacctaana
<210>13
<211>652
<212>DNA
<213>Homo sapiens
<400>13
1 caggcccacg ccaccgtgcc tggctggaca tgtaaatgtg aagtgaatgg ttaaacatgc
61 agctagctga aagcatggca gaccctaaca gaaaagctac agtgtgtttt tgcagctatg
121 aagtgaatgg tttcctgggg aaaattgtga ctttgtataa ctgttgttga aaccagaata
181 aattatattt cacttgcata tgcttaaatt attaaaattt tcagaagtca gtgatacaga
241 agtactattt tgcaatgtta atctgtttga gtctttggag aaagtggttt cattataggt
301 acatagtgca ctgttaatat tttaaacaaa tagttcactc ttccatttaa gggatagcag
361 ttccttgtat aaaatgactg gatgtgtata aaggaattat gttgtcatgt gcctttaacc
421 agctttagta attactataa tctcatattt atgatagctt tgttaggtga caggaccaaa
481 tgacaatatc ttatgctttc tcatcacttt agaatttatc attatgtaca ttactggggt
541 tttagcattt cctaatgtga agttttaatc actcttaagt atacattctt ttctggatca
601 cttaaattaa atatttttat aacctcttaa aaaaaaaaaa aaattaactg gg
<210>14
<211>292
<212>DNA
<213>Homo sapiens
<400>14
1 cgtcgaccga gaagacgaca gaagggcaaa atgatgagat tccacttaat tggtccgtgt
61 ttctgaaaca catgatattt gtggaaattc tgagcaaact atagaacaca ccccagggct
121 aatttgagat aagtacaatt aagttaatct acatatatct aaaaggtttt ttttgtaata
181 agtatctgat ttccggaaga tttactttac tcaaggaact ttaaaggttg tatcccccac
241 caaaaaaaag aaaaaaaatc agaaggccaa ctgcgcaaac aaaaaaaaaa aa
<210>15
<211>522
<212>DNA
<213>Homo sapiens
<400>15
1 tttttttttt ttttttcctt ccttatttta cagctttatt actgatttcc ctctaaaaag
61 aaactcaagt gtggagctga ctcatctata gattaaggaa tcacaaagta ccatagtcac
121 ttaacaaatg caaaaaaaaa aaaaagcaat tgttttacct ttcaactgct gatgttatgt
181 acaatctatc acaaaaagca tagctgggca ttcatgagct gattatatca accctctaga
241 attttaaaaa cgagcccatc taagcatatg gatactctct taccttacaa agtaaaagca
301 ttttctattt taaaattttt atttctgggt aaaacaaaga attcctagag gtcaaataat
361 ataccaaact taaagcacta ttaagtcatg aaacaaagaa gattctgagg tagaagtcaa
421 gcaatataat ccacagtttc aggtattccc agcaaccatc gcatttcgca agtaagatct
481 atttttttta aagtctcaac ttccaaaaaa tcaataatcc at
<210>16
<211>475
<212>DNA
<213>Homo sapiens
<400>16
1 atctgagaag gttctggttg agatttattt agtcaacaca cacacattaa gcatttaatc
61 tgaatagtca catttccaga cacttggcat tattcaacga acaaagcagt tgaaagtttc
121 tgggcctcaa tttcatttgg ggagtcagaa actcacagta gacattataa gcagtagaca
181 ttatatgtca cgagaatgta acatatgatg cctttgtgtc ctcctctagg taccagatgc
241 atcctcctag attctgagaa agatgaagga tctcagaacc aattataact tttaagtgaa
301 tgcagaaaaa gaattttaga aaattcaaca ttactttatg acaaaatctc aaaacaaatt
361 acatatagaa catacatacc tcaagccaat aaaggccata tatgacaaat ggacatctga
421 catcatgcta aaccttcaaa agttgtaagc cttttttcta agagttatag taagg
<210>17
<211>504
<212>DNA
<213>Homo sapiens
<400>17
1 tcagttctgc acaatcctca ctttatcccc ccgaatcccc ccaccagagg gccatggagg
61 gtcccactgg gcaccaggcc tggtccatgg ggagcgacaa agaaccttct gctgtcatta
121 accgcgatgg tttatcacaa tgatgaccct ccccgaggcc tctcctacgg taacagcaac
181 atctacggtt ccacacccac agggaaaacg gattcagagc ancagcaggg ggcgggcagg
241 ctgtggggcc gacagtgaac atggaggcag gctggtggct gggagggctc catgtgggcc
301 tggctgagga gccagggatc tggagtcaga accgggggaa ggcagctgga acccagtagt
361 ggacttggaa ttccgcaaat cgtcctcaaa cgtcttcagc cagaagcgac ggcggcagcg
421 gcgcctgcag acaggcgtca ggaagcttcg ctgctgaggg tatttctcag gctgggctgg
481 gctgggaggc ccaggtcctt gcag
<210>18
<211>451
<212>DNA
<213>Homo sapiens
<400>18
1 ttttttttgt atatatgtac agaaatttat tataaatttc attgataaaa atgatgtgca
61 gcacataatt tataaataaa atatacaatg ccatttattg taattctaga aaagtttttt
121 acagaatgct tttgttgatt tttgctgact cttctatcca acctgtttgc aattcaacca
181 tgatatgaca caaccagact acaaataaat gtttgttact atctgattca acaaagcagt
241 cactcatttc atggaatgga tatgggctgt tactcagtga aaagacacat gagatggttc
301 agtgctaact cacccttctg acagaaaatg ccagaaagtt tccaccccat tgacaggctt
361 ggagtcttga gtaccatctg ggtgggtggg ctggcactgg cacgaaacct ctccactgct
421 atcctgacag agatggggca tgtacttccc a
<210>19
<211>641
<212>DNA
<213>Homo sapiens
<400>19
1 gaaagatttt tttgtttatt tggtatgggg tcttctgtag ctcacacaaa attattggga
61 aaataaataa cccaggggag ccccctttgc ccacggttcc taggtcgcgg agtcacagca
121 gccccaaagg ggcaggaacc tgggaatccg aggggtcccg gccctggcgg tcacttgccc
181 agcaggaggc cggcgcgcag cacgcggggc acgcggcgga tggtcagcga gacccgggtg
241 ccgcgcacca ggcaggctcc cggccgcgcc gacgggcagg ctgccgcatt gggcggcgag
301 gaggcggcgt ccagcgcgtc tacgcgggcg gcggcgatgc cgtggagaag acgcgtgtag
361 gcggggccgc ggagcaccag caggctgcgc ggttccagca ntaacgaggt ggtgggccgg
421 ggcggaggcc gaggctgttc tgtanggtca tcgtcctctg gccgccgcgg ctcgtagaag
481 tccagcacgg tgtgggagcc caggctgatg gtgctgacag tcnggtagta cagtggtccg
541 tcctcgtggg gcatgatgcc ctccacaggc agatactggt tcacgatgac attgttatgc
601 tggatgcctt catagatgct gatgtttgac acttttgtca c
<210>20
<211>599
<212>DNA
<213>Homo sapiens
<400>20
1 tttttttttt tttttttgcc tttgacaagt tttttattgt gaaatattgg atctaaaaaa
61 aagtacaaaa tgtcaacagt cagatgtgta caagtacagt atttcaatag tctatacatg
121 tgaacagctt aacaggttca ctgcagaatg catatttaga ccaatataat ctaaaaagga
181 ctgcaatatt cacaggctat taatacataa ataacttgag tcaaaatgac ccttactggt
241 aaatgctaat taatagtgga aaacaggcta aaagcacaat acaccacacc atagtatctc
301 cttacaggtc cacatttaaa gaatatcagt ttattatgtc aaaacactac agagatccag
361 gactgggact gaattactaa tttatacaat tgcaaaaaaa aaaaagttat aaaataaggt
421 catggaataa agaagggtat ttaattagga ctaaatactg tgaagcccat gaaagggttt
481 tagccttgga atttacaaag ctaaattatt agactgttaa tgttagctaa cactaaaact
541 gaattacctt ccattttaat gttagctaat attaatattc aactttttaa ttttgatgt
<210>21
<211>478
<212>DNA
<213>Homo sapiens
<400>21
1 tttttttctg tagagacagg gtctccctgt gttgcccagg ctagtgtcaa actccaggcc
61 tcaattctcc taccttgacc tctgaaagtg ctgggattac aagcatgagc caccatgccc
121 cacctaaaac attttaagtg ccatcaagag aaatcacagt attttaaagc tggcttattt
181 cagatgatgt ttcagaacaa agaattttcc tattttgagg caaacagaag cagaaaagaa
241 aactgcatta gccaggaaga tgtgaaagac acaaagattt tataaatctg tgaaatccag
301 aaaggctgaa ggaaaatttg gaattgctga aggaaaccaa aaagattata tcaataatac
361 aattaatagg gtatatgaga gacatatttt taaaataaat attctctata taaaacaaag
421 catgcttcaa acaaatacaa tatatttatt acacaaaaat gtcataactg acaaactg
<210>22
<211>547
<212>DNA
<213>Homo sapiens
<400>22
1 aagagcgaca gcaaacattt attgagagct gtaaaatgtg tttcacccaa ttgtaatgta
61 aagaatcgaa aattttaaaa aatttccaaa aaaattacat gattgtagtt ggtaaaaatg
121 cacccatttc aggaaggccc ctgtgcctgt gtataaatga tgtgtcttga acgctgctgg
181 gaagctctat tgctgagatt gagaagacct tagaaacgga aggacactct tcatcagagg
241 acattaattc atttgacaag catctagaaa atgaactact tggggaaagg aaatactatt
301 tgcttaacag aaggctatat tttagcttca ttggggcgta tcacttagat ttctttcgat
361 ccctcaatgt tcactaaata tagtttaaaa actgagatgt gaaatacaga cacaaaatgg
421 aaattttaag ccaagcctac ttttccccac aaaggcattt ttgtgatacc ccaccatatt
481 aatctacaga atatttgtaa ccaatatttg tgaactaatt ggatgttttg tgattntaat
541 cacaata
<210>23
<211>469
<212>DNA
<213>Homo sapiens
<400>23
1 tttttctgtg aaacacgatt aattatataa aacaaggtgc atccaaattc agtgtcagaa
61 gagctttgta tttcaagggc taaggactgg accttctccc ttaaaacctc cttacccaac
121 ccttgctaag gactcaggcc tggggcagag gctgtcagtc tctaaagacc ccagccctgg
181 accaggacgg ggagaacaag tttggtgcat agcgtggggc tagggcagtg ggtcaggcac
241 catcccttct gcaagtggcc acaggtcacc acagtggggg aaggtgggga gcttgaacct
301 ccaaagatag gttcattgta ggaaaagcta gaccaatgcc ttgggtagaa acacccaaat
361 ccctctacaa caactaaagc aagaatgtgg aacccatcga aatcttcccc caaaatgcca
421 accataaaat agattctaca gaaataaaga aaggaatccc agcactttg
<210>24
<211>647
<212>DNA
<213>Homo sapiens
<400>24
1 tttttttttt tttttttccg ttgagaaaag ttttatatgg aaacacatac tgatcatgaa
61 cacaataaac agggagggaa gctcgggctc agccaggaaa cctgccacaa ggaagatgtt
121 tggaactatc caggagtagt gtcaaacact aacaccatat ttacaagtct aatttggaac
181 ctggcccttt ttaagtgcag gaggaagttg gaggagaagc catgcatgaa gtaaaaaata
241 catatacaca gacataaaca cccacatttt cacacttctt ttgcccattt gttccacaag
301 gaacaaatga gagaaagaag cccagcctct ctccctggag tccgtatact tactacagat
361 ttgttagaga agccccagat ggggcttgga ttcaaggagc agacttcact cttactggcc
421 ccactcacgt gacaattcac caagggaatg cttgggacac angcatgaat gtccggaaga
481 ggcaagaaaa tatatggctc acttaactta cacattacac ataagaggcc tcagtttctc
541 caagtacttt cacatttgat ctttagcaga cttctaacct gcagagacag agctcagcca
601 agctgtgaga cacagaagaa agcagcattt tgatccagtg tgcactg
<210>25
<211>559
<212>DNA
<213>Homo sapiens
<400>25
1 tacatatgca aaataatctt gtttattaga aaatgaacat tcacaatgta aagtagaaat
61 taaattacaa ataagtcttc aatggtcaaa aatggtgccc agaatttctt ctcaaaataa
121 gttaatctat tcttaaacag atgcaggtaa gtagtttatc cttgtcaaaa caaaaaatta
181 acagatttca ctttatgtaa gagataaggt ttatatgaac aagaagagtt tatttttaaa
241 tgtaaagctg aacaagatag aatggagatg ctttgtgaag ttataaatcc cttcaaaata
301 tgaaaatcat cctttcaagt ttaaatttgt tttcaaaaac atattttctc ttcttaaata
361 tgtactactc ttaacaatat atcatacctt aaaatctcct gttgtcattt tctctttata
421 cgcaacttgg cctactcaag aagacatcaa catggtgatt ttttttcctc aaactttatc
481 tccttgctct tttcccaatc ccatgttact tcctttcgcc ttcttttgtg ccaagatgag
541 caaggtataa tcctccaaa
<210>26
<211>243
<212>DNA
<213>Homo sapiens
<400>26
1 ttttcactga atttctcttt tatttaagca ttaagaaatt ggagcatagc gacttagaaa
61 actttctcaa aatgtcctta acagcaatga aaagatatca tgtgtcacgt gtaaaacctt
121 ttttctgttt gttttttatt gaggctcatt gaccagggaa acataaatgt tccactcatc
181 tgcttttaaa gtagagaaca aggcccacaa actctattta ataaatacaa attactaaaa
241 atg
<210>27
<211>494
<212>DNA
<213>Homo sapiens
<400>27
1 ggacgcgtgg gtccggatct ggaggaagta caggaagaag actttgtttt gcaggtggca
61 gcccctccca gcatccagat aaagcgggtg atgacctacc gtgacctgga caatgacctc
121 atgaagtact cagccattca gacactggat ggggagatcg acctgaaact cctcaccaaa
181 gtgctcgcgc cggagcacga agtccgggag gatgatgtcg gctgggactg ggaccatctg
241 ttcactgagg tgtcctcaga ggtcctcact gagtgggacc cactgcagac ggagaaggag
301 gaccctgcgg ggcaggccag gcacacctga gcccgtcacc catgctctag acatgaagaa
361 atgcaatgag cttaaagcta aagaagcttg taagcagctc cgaattttta cctggaatat
421 tttgtaataa aaatatttat attcagtcaa ccacattgga taattcaatt gcaataaatt
481 gcttattctg tgcc
<210>28
<211>918
<212>DNA
<213>Homo sapiens
<400>28
1 ggcggctcag cgacgcggct tctagaaccg ggtgattgaa ctaaaccttc gccgcaccga
61 gtttgcagta cggccgtcac ccgcaccgct gcctgcttgc ggttggagaa atcaaggccc
121 taccgggcct ccgtagtcac ctctctatag tgggcgtggc cgaggccggg gtgaccctgc
181 cggagccacc gctgccagcg acatgttcaa ggtaattcag aggtccgtgg ggccagccag
241 cctgagcttg ctcaccttca aagtctatgc agcaccaaaa aaggactcac ctcccaaaaa
301 ttccgtgaag gttgatgagc tttcactcta ctcagttcct gagggtcaat cgaagtatgt
361 ggaggaggca aggagccagc ttgaagaaag catctcacag ctccgacact attgcgagcc
421 atacacaacc tggtgtcaga cagctatgac tatctccaaa atgcacctcc tggatttttt
481 tccgagactt ggtgttattg gttttgctgg ccttattgga ctccttttgg ctagaggttc
541 aaaaataaag aagctagtgt atccgcctgg tttcatggga ttagctgcct ccctctatta
601 tccacaacaa gccatcgtgt ttgcccaggt cagtggggag agattatatg actggggttt
661 acgaggatat atagtcatag aagatttgtg gaaggagaac tttcaaaagc caggaaatgt
721 gaagaattca cctggaacta agtagaaaac ttcatgctct gccatcttaa tcaggaaaaa
781 ctagaccaga cctctgttat cttctgtgaa atcatcctac aagcaaacta acctggaatc
841 ccttcaccta gagataatgt acaagcctta gaactcctca ttctcatgtt gctatttatg
901 tacctaatta aaacccaa
<210>29
<211>484
<212>DNA
<213>Homo sapiens
<400>29
1 aacaaactag ccctcaagac gggaatagta gccaagaagc agaagacgga ggatgaggta
61 ttaacaagta aaggtgacgc gtgggccaag tacatggcag aagtgaaaaa gtacaaagct
121 caccagtgcg gtgacgatga taaaactcgg cccctggtga aatgacgccc ctccccacct
181 gcccatggcc tgggactctc tgcgatgtac ataactattt aatgcagcgg cagcggcgac
241 agccttccct gagaggactt aaaagcagaa ggaaaccgag atgcttcccg cagccgtgga
301 cgattctcca ggactctttt tttaccttga gcacttgcct cgtgagactt catagaacag
361 tggtttactg tccccccctt ctcacctcct cattctctct ggctctttct gtcttcctct
421 tctcaccctc ctccctcccc ttagccatca cttctgggaa gtaaagaact tgacttagtg
481 gccg
<210>30
<211>2241
<212>DNA
<213>Homo sapiens
<400>30
1 agtagaaggc tcaaacaacc caaggttagt tggtctttgt gtgacagtgg gaagaagtgg
61 agagaacact ctgtctcccc gacttccttc ttgaccctcc ttccgtatct ttcattcttc
121 cccactggcc tttccagatg aggtccaaaa gtaagctcac tcttcaggga aaagtgatac
181 taaacccagg cgtgaggagt catccttctg ctcttacttt tccctgcatt tccttaggct
241 ggcccagact ctagattcct gggcacttac ctgtatctga gacccttaga cctcctgtct
301 cagtttctcc acctgtgaga tgggcagtca cacagatgag tgtgcatgag ggaatcagaa
361 gcccatctgg gttgactcag aacccgggct ctccccctgc tgtgggtgga aacaaaacac
421 cttttcacag aagctgttgt ccctccccca tccctgatac catcacccag aagctgggtg
481 gggaatggga agctggagga gggggttaga gttagaccaa tgcgaggagc tagtaagagc
541 tcgcctctcc gtgacttccc cccatgcaag ggtggcccaa ggcttcccct gatatccaaa
601 gcaggacagc agccccttgg tgggattcta actgctgcta ctctgtttct ttcctcactt
661 tgctttccaa ggtggtatgt gatccccagc tcaggcctgt gcagacagga aattctcccc
721 tgcagcaagt aggggaggtg ggttgtggga tgtgacctcc ttccagatat caggcagtga
781 gtgtaaacct gccacctcca gccctgatcc attctcacct agcggctaca ggaagctgtg
841 tctgttcgat ttggtgggag gagatgtgca gggagctgta tcttgtcctc cgcttgtgaa
901 aaactcaagg atgtggagaa gagtagaccg tggaaccctg ctcttctgca gccaagctga
961 ggggcaggat gcgtgtggga cagtggtaga gaagcagggg ataaactcat aggctgcaac
1021 aaaggtgact ctgtccctgg acactgcctc cgtactttct ccttgcttca ctggccacag
1081 catctccctc cagccctcgc tatgtgcctc tgccatcttc acccatcatg gagcagaggt
1141 gaggagaggc agcctgggaa tatggagacc agtgaaggac caggcctgga gagcacaggg
1201 tcctacctgg gcatccagca gaggagcccc taaaggccag gagcacccca agaggaggga
1261 gggcagccag cctccattga cggcgagcct ccagccctct cctactttga tcaccatttc
1321 tctccaggct ttctgcctcc gagatgtggc accatagtgc ggtgccctgt ggcttcaccg
1381 ccctacttcc acctccgccc agcctgtaat gtttatataa gcagcctcaa ggaccaagaa
1441 ccatctgcga aaggacacac acaggaaatt cataaaagaa atctgaatgg ataaaaccat
1501 gaaaaaaagt atgcttcatt agtaattaaa gaaaggcaaa tagagctgga agcatttttc
1561 ccttagcaaa ccataacaga aaaaaataag acccaatatt ggcaaagaga ctactgaaaa
1621 aacattccca tacattgcgt gtgggagtat acatcggtgc aggcttcctg gatgacagtt
1681 gggtgatatg tgtcatgtgg cctaaaagcc tccatgtcat ttgacctacg aattctatct
1741 ttgggaattt atcctaagaa aatacttaag gatttagtta gtgataagat gttcatccca
1801 gcattgcaat ggagaaaaat gggaagcaat ggtttggttg ggaatttatt ccttttctgc
1861 tgtaacgaaa gtttgcaata ggggattgct taagtaaatt attgtatctc catccagatg
1921 gtggagtacc gcgcagacat taaaagtcat gtaaaagaac atctgactga aagaaaaatg
1981 ctccttgaat attaaaaggt tgtaaaaata gtgcatgtta tgtgatttca attttgtttt
2041 ttaaaatatg ggtgtatgct tgtatacgta gagcagataa aaaagacgga aggcatacta
2101 aaaaatgttg agtggttatc tttgtatggt ggaacaaagt cactgtaatt ttcatctttg
2161 gtttttctgt aatttccaaa ttttccacat tttgtatttc atataataaa tataatttaa
2221 gaaaaaaaaa aaaaaaaaaa a
<210>31
<211>1599
<212>DNA
<213>Homo sapiens
<400>31
1 accgggactt gcaggaagac gaggggtcgg aggacgccag cctgtatggg gagccgtcac
61 caggccctga ctcgcccccg gcggagctgg cctacaggcc accactctgg gagcagagtg
121 ccaaactgtg gggcactggg ggccgggcct gggagccccc agctgaggag ctgccgcagg
181 ccagcagccc accatatggg gccttggagg aggggctgga acctgaacct tccgccttct
241 ccttcctctc tctgcgccgt gagctgagta ggcctgggga cctggccacc cctgaaagct
301 ctgcagcggc cagcccgagg cgtattcggg cagagggggt accagcctca gcctataggt
361 ctgtctcgga gcccccaggc taccaggcac acagctgcct gtcccctggc gccctgccca
421 ccctctgctg cgacacctgt cgccagctgc atgctgccca ctgtgcagcc ctgcccgcct
481 gccgtccagg ccactcgctg cgtgtgctgc ttggcgacgc ccagcggcgc ttgtggctac
541 agcgtgcaca gatggacact ctgctctaca acagccccgg ggcccggccc taggccagct
601 gggccccagg tcaagggctc ttgggagaca gctcccgtgg tgcttctctt tgctggggag
661 gggtctgggc ctctggttgc cttgtccctg ggggagtcct gggcacagca gggggttgga
721 gagtccaggc caacccttgc tggaggtggg actctctgta tatggaaggc tccaagggca
781 gcaggcctaa gccggccagc aggagccttt ccacatttcc ccaccctcgg aacccagacg
841 catgcccaca tgtgctgggt agaggggtcc agcctgttcc agccagtgcc tgaacctgtc
901 cctgagaagg accctgtgcc ccaaggcccg tggctcctgg cctgctccct gcagtacgga
961 gtacggggct caggacttgg gggtcctcca cgtgtcccag ggattctcca cacgagtgtc
1021 ctcaagggcc tgttggtgac accgagctcc ccccacccca agctctgctg gggccaatct
1081 gcggggctct gccgggtgca cggctgacct ggcttcgggg acgccggccc tccccttggg
1141 tgtcacagca gctgggccca cagacaggaa atgacctgac ctcaaaccag atgtggaggt
1201 gcacgagagg cgcaggaatg ggcagtggct gacagctggg aaggccctga gcgtcactgc
1261 tgctgggcgg aagggcctgt ggacagccca cccaggcggc ggggtcttgg tgggtccagt
1321 ggtaatggtg cccctcccct ccattccctg tgggtgcagc ccacaggctg gcgaggaggt
1381 gcctctgccc taccctcggg tctggcgagg gagggggcag ggtgcatcca ctgtgacagc
1441 ccctctgggc ccacaactcc ctgaccctgg aagctgagcc tgctttcctg accctccgcc
1501 ccaccccctc ctccccagaa cactcagagg gacggtcaac atcagagcac attaaacgct
1561 ggtgaaacca aaaaaaaaaa aaaaaaaaaa aaaaaaaaa
<210>32
<211>1060
<212>DNA
<213>Homo sapiens
<400>32
1 ggctgccacg gggactgaag atggcgccgc gaggtgagat tccggaggta aacggttgtc
61 ctccaccccg ctggaaatcc tgttctttct gaacgggtgg tataatgcta cctatttcct
121 gctggaactt ttcatatttc tgtataaagg tgtcctgcta ccatatccaa cagctaacct
181 agtactggat gtggtgatgc tcctccttta tcttggaatt gaagtaattc gcctgttttt
241 tggtacaaag ggaaacctct gccagcgaaa gatgccactc agtattagcg tggccttgac
301 cttcccatct gccatgatgg cctcctatta cctgctgctg cagacctacg tactccgcct
361 ggaagccatc atgaatggca tcttgctctt cttctgtggc tcagagcttt tacttgaggt
421 gctcaccttg gctgctttct ccagtatgga cacgatttga agtacagaat ttcagccagc
481 agcccatcag gctgacacca cacatattgc ttctggtact ttagcccaca ccagtgagaa
541 ttggtggggc aagttgtcct gagaaaggct gtgtggcttt tcttcagcac agacatttgg
601 gcaagcaact cagcataagg ccagtgggta ccatcttcta aaccaggacc atcagcccaa
661 gagactcttc tacactccag tatagggagg ggcaaggtta ttcccatcct gccccttctc
721 agaaccagtc ccctgctgac ctcaagttct cctccttgat caccgtggcc agagcatctc
781 gtgtggacca tctaggctcc ttgggcttca agcaggacct gagccacatg ctccctgtac
841 gagctgtgct atacctgtcc cacatgagca cggagagcct catgttggtg ggtttccaga
901 gtgatgtgaa agcctctcac cccaatcctc ggagactgag ttccacaact tttttagtag
961 ctcatagtgt tatttttcta ctctcttcat gaaactaact ttattttata ataaatatat
1021 attttctgtt gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
<210>33
<211>1481
<212>DNA
<213>Homo sapiens
<400>33
1 ggcgtttgtg gccgtccggc tccctgacat gcagatttcc acccagaaga cagagaagga
61 gccagtggtc atggaatggg ctggggtcaa agactgggtg cctgggagct gaggcagcca
121 ccgtttcagc ctggccagcc ctctggaccc cgaggttgga ccctactgtg acacacctac
181 catgcggaca ctcttcaacc tcctctggct tgccctggcc tgcagccctg ttcacactac
241 cctgtcaaag tcagatgcca aaaaagccgc ctcaaagacg ctgctggaga agagtcagtt
301 ttcagataag ccggtgcaag accggggttt ggtggtgacg gacctcaaag ctgagagtgt
361 ggttcttgag catcgcagct actgctcggc aaaggcccgg gacagacact ttgctgggga
421 tgtactgggc tatgtcactc catggaacag ccatggctac gatgtcacca aggtctttgg
481 gagcaagttc acacagatct cacccgtctg gctgcagctg aagagacgtg gccgtgagat
541 gtttgaggtc acgggcctcc acgacgtgga ccaagggtgg atgcgagctg tcaggaagca
601 tgccaagggc ctgcacatag tgcctcggct cctgtttgag gactggactt acgatgattt
661 ccggaacgtc ttagacagtg aggatgagat agaggagctg agcaagaccg tggtccaggt
721 ggcaaagaac cagcatttcg atggcttcgt ggtggaggtc tggaaccagc tgctaagcca
781 gaagcgcgtg ggcctcatcc acatgctcac ccacttggcc gaggctctgc accaggcccg
841 gctgctggcc ctcctggtca tcccgcctgc catcaccccc gggaccgacc agctgggcat
901 gttcacgcac aaggagtttg agcagctggc ccccgtgctg gatggtttca gcctcatgac
961 ctacgactac tctacagcgc atcagcctgg ccctaatgca cccctgtcct gggttcgagc
1021 ctgcgtccag gtcctggacc cgaagtccaa gtggcgaagc aaaatcctcc tggggctcaa
1081 cttctatggt atggactacg cgacctccaa ggatgcccgt gagcctgttg tcggggccag
1141 gtacatccag acactgaagg accacaggcc ccggatggtg tgggacagcc aggtctcaga
1201 gcacttcttc gagtacaaga agagccgcag tgggaggcac gtcgtcttct acccaaccct
1261 gaagtccctg caggtgcggc tggagctggc ccgggagctg ggcgttgggg tctctatctg
1321 ggagctgggc cagggcctgg actacttcta cgacctgctc taggtgggca ttgcggcctc
1381 cgcggtggac gtgttctttt ctaagccatg gagtgagtga gcaggtgtga aatacaggcc
1441 tccactccgt ttgctgtgaa aaaaaaaaaa aaaaaaaaaa a
<210>34
<211>336
<212>DNA
<213>Homo sapiens
<400>34
1 gccactgcga ggccgtttta gaaaactgtt taaaacaaag agcaattgat ggataaatca
61 ggaatagatt ctcttgacca tgtgacatct gatgctgtgg aacttgcaaa tcgaagtgat
121 aactcttctg atagcagctt atttaaaact cagtgtatcc cttactcacc taaaggggag
181 aaaagaaacc ccattcgaaa atttgttcgt acacctgaaa gtgttcacgc aagtgattca
241 tcaagtgact catcttttga accaatacca ttgactataa aagctatttt tgaaagattc
301 aagaacagca aaaaaaaaaa aaaaaaaaaa aaaaaa
<210>35
<211>1424
<212>DNA
<213>Homo sapiens
<400>35
1 tagtgtagac ggaagctcct ttggtgccag attataaagg tagaaatttg tggaggacat
61 cgaggagaca gaagacatgc tttatacatg tttcttttta ttttaaaaag ctttgaaata
121 catttgaatg aaaaactaag cagttgctta tgctgtcagt acctgtctct gctctttaaa
181 aagtaagcca ctgcctctta cctaaaggtc agcatgggct ccatcataca gcggtcaggt
241 ccatcataca gcggtcaggt ccatcataca gcggtcaggt ccatcatacg gcggtcaggt
301 ccatcataca gcggtcaggt ccatcatacg gcgctcaggt ccatcataca gcggtcaggt
361 ccatcatacg gtggtcaggt ccatcatacg gcggtcaggt ccatcatacg gcggtcaggt
421 ccatcatacg gcgctcaggt ctgtaactct tgacaggaag gtgcagtagc agaatggcga
481 gatgcactga gggtaaccag atgatctcat gggacttacc cagtgttagc accaaagtcc
541 catgaactga ggtgttaaaa cagaaaggcc tgtgtcctca gaacccctcc aagtcactgg
601 ccaactatga ctcatgtttg gtgatgttgg atgccagtta ataaagtcca gaacttaagc
661 ccaagaagag agctcagaag tgtttgtgtt actctttatt tattgaggaa taatttacag
721 atagtaaaat ttacccctta tatctgtaca gttttgtgaa ttatgacaaa cttactcagg
781 ttgtgtaacc accagtgcag tcaaggcata gattatttcc atccttccag agaagtctct
841 gggacctttt gtagtcagtc ccctcatgga atgttttgat caacgtagaa atgcctggag
901 gctgctgggt gggaggctga ccactcagcc atcagagcgt cctgtgtctc tgagccgtgc
961 accccattgt ggcatggcac tggttgctgg acattcttct gcgggcccac tcacacatct
1021 gcctgcctgc ggcctgtctg tgtttgtctt actcatgctc ttggagccgt agcgaatgtg
1081 tctaatattc cttccacgtg gtagattgaa acttcaccaa aatagccctg tgcgcctact
1141 ttgctcctga acatctctga tttctccggc tgtccctgtg tggagttctg gcagatctca
1201 ccagtctaca tgacctattg gaaatgccag tgggtcattg tggcattcag acacataatt
1261 caaaactgca aatggtggga ccgcactaaa gaatttttag taattaaagt acaggaggct
1321 gaggtgggag gatcacttca gcccaggagg tcaggtcaag gctgcagtga gccatggttg
1381 caccactaca ctgtagcctg ggtgacaaag tgagaccctg tctc
<210>36
<211>3174
<212>DNA
<213>Homo sapiens
<400>36
1 acagtcttcc agctccacat cctgagagga cgcttctgga gccgcgactg cccggggttg
61 tgccggccgc cgctgccgcc caggccgcct cagctctcct ctgcgccggc ccgctcactc
121 cgcccggccc cagccctagc gctggccgcg accccggcgc ctttgaaact tctgctggtg
181 tgagtgccct cgggggttcc ccaggaatat cgatacaaca ccaacaggag atcatgaatc
241 agacagataa aaatcaacaa gaaatcccat cataccttaa tgatgaacca ccagaaggtt
301 caatgaaaga tcacccacag cagcagccag gcatgttgtc ccgtgtgact gggggtatct
361 tcagtgttac aaagggagct gttggtgcca ccattggtgg tgtggcttgg attggtggaa
421 agagtctgga agtgaccaaa acagctgtta caactgtgcc ttccatggga atagggctgg
481 tgaaaggggg tgtctctgct gtggctggag gtgtacagct gttgggtctg ctgttgtaaa
541 caaagtgccc ttaacaggaa agaagaaaga caaatctgac tgaaatatag agatacactt
601 gcgctccaca gcactgtaat gccagtggca ttgaattgct aaattatgga ctacaaccaa
661 gtcaactgtt ttggacgttt atcttctaaa ctgctgtgtt gaaagtattg atgactggct
721 ttcatctaaa aagaagagac caatacgagc acagtatatg aaggtttctc atacttaagt
781 tccaggtttt tatctggtaa aatgttacac ttactcggtt gtaactgaag atatggtatg
841 tttgaatatt tactataagt ctttcagttt gactaaaaat gtgaaagttg aatttagtag
901 atgatcttca cagttccata tgtataatgt gccaggtaac tcacctgccc cttaagaagg
961 gaaccttgaa ttacataagc cgtacctttg atgtgcctaa tagtttcaga tgtcttagtt
1021 ttttataacc atagttgatt aggccaagag gcattcattt cttatttaag ctggcaaaat
1081 tagcaggaat tagagaagtt taaaaagata aatggtttta tgataatgtt aaccatcttt
1141 tgttagtaaa tatgcgttct attattttaa tcattgatgc cttacaaaag aaaacatctt
1201 ttctaatacc ctgaatatgt gctgttctta gaatcatcta tggattcttt taaaggttgt
1261 ttgtgaaatt agttttccct ttttagaatc tcaggagtag tggggtaaag acatttcctg
1321 ctgtcagtgg ataagacaga gcttgttaac tgttttggca gtagttaaaa tcaaattgta
1381 cacttctcag cctgggttca tgcttcatca ttaatacacc tcacagtgcc taaggaacat
1441 ttacttactg gtcagaaggt attttggaag agtttcatat taaggaggaa caaataataa
1501 ttttaagttc ttaaaaatta cctaaaacac cccaaatata aaaagaagcc ttcacaccta
1561 ttctgtcttt aggatgtctt aaattattag cagtactcct ttttttaaaa acactgtaaa
1621 agtaaccaca aatatgtgag gacttactat tttaaatgga atggaatgag ctccatagat
1681 tagttttgaa tataaagtat ataaaagtgc atcagtggtt tatataggct ttaaaaacat
1741 gttatcttac agtcctttaa agcagccata gagtttgtat catttttcaa gccaatttca
1801 gtcagggatt tgaattgttt gattatggat gataaatgtg tcatatctta ttaatatgtc
1861 tcatgtctcg ttccttctta atatgattta gctggaattc attttctttt cgtttcatgt
1921 ttaatttcat aaaacgttta acaattggca tatatacttg gcattcctgt ccaccaagga
1981 ttgtaatcca agcctgggaa aatcttaaat ttctttttac ttaaatctgg aaatttgtct
2041 ccattctgcc accttttttt tttttttttt ttatagtgga ggggaggaaa ggggggtgat
2101 accttgcagt aagtaagtcg aaatagcatg cctgaaaatt tgaaacagac cattctaaca
2161 cccgaggctt gtttataaaa tacttgagaa ttacattaat gtggaatcaa cagatgcaga
2221 agaatataac ataactttta aaagctttca taaataccag cagcaattgt aagcaaatct
2281 acaaaggttc ttgaaccttt ctattatata caaaactgaa aagtcattaa ggagttcaac
2341 taatcaggaa ttaaatggtc atttatttca tgcagtatga tttaaggtat ttcttgagat
2401 tctggtcaaa tgtcataatc agcaaacggg attaaaaaaa aaactccaaa atcactaaat
2461 aattatctaa ataatggtat tggagaactt gtttcctgct atttggaaga gattgttgct
2521 tcattgctag tttgtatttc taacttctac agttatagac tccactgtgc tttgtgtctg
2581 aatttctcag tatagacatt ttgtttactg tatgcttgca tatttatttt caactttgtt
2641 tgtctttaaa attgcttgag gaaagatggt tgtaattaat ttctgctaca gaaaagccac
2701 ctggtacgtt ttgtctcatc aggattgttt taaattctaa actataagtt tgttcagagg
2761 ggcttttgca atgatagcag aaaactgtac aaatgtacag ttagttatag aggttcttgt
2821 tgaaatgaac ttaccatctg atgatatgta tgtacagctg tgtacttgag tcttttttag
2881 tttacttaga aagactagca gtttgacctg ttaaacagga ctagttcaag tcaagaaact
2941 aaggttgttg tatacacctg gaggcatctg ttattcagct tatcctttga gtgggaattt
3001 ggcacaatga ggataaactt atgtgaccca cttgaatggc tgatctaata atgttgacat
3061 tatgcattct gtacttagtg aaatgtcaga tgaaaataac tgatgaataa tttttttgta
3121 ttaaagggat gggaaaagaa cacatgaatt tgttaataaa gcactatgat ctgc
<210>37
<211>2076
<212>DNA
<213>Homo sapiens
<400>37
1 cgcttttaaa atgattcgta ccaacgtttc tcatgtggtt ggccagttgg atgacataag
61 aaaaaaccct aggaagtttg tttgcctgaa tgacaacatt gaccacaatc ataaagatgc
121 tcagacagtg aaggctgttc tcagggactt ctatgaatcc atgttcccca taccttccca
181 atttgaactg ccaagagagt atcgaaaccg tttccttcat atgcatgagc tgcaggaatg
241 gagggcttat cgagacaaat tgaagttttg gacccattgt gtactagcaa cattgattat
301 gtttactata ttctcgtttt ttgctgagca gttaattgca cttaagcgga agatatttcc
361 cagaaggagg atacacaaag aagctagtcc caatcgaatc agagtataga agatcttcat
421 ttgaaaacca tctacctcag catttactga gcattttaaa actcagcttc acagagatgt
481 ctttgtgatg tgatgcttag cagtttggcc cgaagaagga aaatatccag taccatgctg
541 ttttgtggca tgaatatagc ccactgacca ggaattattt aaccaaccca ctgaaaactt
601 gtgtgttgag cagctctgaa ctgattttac ttttaaagaa tttgctcatg gacctgtcat
661 cccttttata aaaaggctca ctgacaagag acagctgtta atttcccaca gcaatcattg
721 cagactaact ttattaggag aagcctatgc cagctgggag tgattgctaa gaggctccag
781 tctttgcatt ccaaagcctt ttgctaaagt tttgcacttt tttttttcat ttcccatttt
841 taagtagtta ctaagttaac tagttattct tgcttctgag tataacgaat tgggatgtct
901 aaacctattt ttatagatgt tatttaaata atgcagcaat atcacctctt attgacaata
961 cctaaattat gagttttatt aatatttaag actgtaaatg gtcttaaacc actaactact
1021 gaagagctca atgattgaca tctgaaatgc tttgtaatta ttgacttcag cccctaagaa
1081 tgctatgatt tcacgtgcag gtctaatttc aaagggctag agttagtact acttaccaga
1141 tgtaattatg ttttggaaat gtacatattc aaacagaagt gcctcatttt agaaatgagt
1201 agtgctgatg gcactggcac attacagtgg tgtcttgttt aatactcatt ggtatattcc
1261 agtagctatc tctctcagtt ggtttttgat agaacagaga ggccagcaaa ctttctttgt
1321 aaaaggctgg ttagtaaatt attgcaggcc acctgtgtct ttgtcataca ttcttcttgc
1381 tgttgtttag tttgtttttt ttcaaacaac cctctaaaaa tgtaaaaacc atgtttagct
1441 tgcagctgta caaaaactgc ccaccagcca gatgtgaccc tcaggccatc atttgccaat
1501 cactgagaat tagtttttgt tgttgttgtt gttgttgttt ttgagacaga gtctctctct
1561 gttgcccagg ctggagtgca gtggcgcaat ctcagctcac tgcaacctcc gcctcccggg
1621 ttcaagcagt tctgtctcag ccttctgagt agctgggact acaggtgcat gccaccacac
1681 cctgctaatt tttgtatttt tagtagagac gggggttcca ccatattggt caggcttatc
1741 ttgaactcct gacctcaggt gatccacctg cctctgcctc ccaaagtgct gagattacag
1801 gcataagcca gtgcacccag ccgagaatta gtatttttat gtatggttaa accttggcgt
1861 ctagccatat tttatgtcat aatacaatgg atttgtgaag agcagattcc atgagtaact
1921 ctgacaggta ttttagatca tgatctcaac aatattcttc caaaatggca tacatctttt
1981 gtacaaagaa cttgaaatgt aaatactgtg tttgtgctgt aagagttgtg tatttcaaaa
2041 actgaaatct cataaaaagt taaatttttg tctgac
<210>38
<211>2005
<212>DNA
<213>Homo sapiens
<400>38
1 tgctgcagcc acaacaagtg ccaccaccag gggccttcca gctgccagtg caggctttcc
61 agagccgggg actggggaac ctgcagtcat cacctcagca attctggtgg cagccaggcc
121 agccagggca gcgatgatga ccgtaatgaa aagtgggatg acagctccac actggtggat
181 gagttgagaa caccaggcag aggcatgtac ctggtctttg atggttcagt ggacctgcac
241 taccattgca gtgcaaagtg caagagttga agcttggaaa ccttcaccta gatttaggaa
301 gattcaggga aaagtctgga tgtccacgca gaagcctgct gcacgagtgg aaccctcatg
361 gagaatctct accagggcag tgtggagggg aaatgtgggg ttggagcccc cacacagagt
421 cccactggag cacttcctag tggagctatg agaagagaac cactgtcctc ctgacatcat
481 aatggtagat ccactggcag cttgcactct cagcctgaaa aagctacaag tactcaaggc
541 cagcccttga gagcatctac agatgctaaa ccctggaaag ccacaagtgt ggtgctgccc
601 aaggctttgg gagcccaccc cttgtaccag cacgcccttg atgtgggata ggaggtcaaa
661 ggaagttatt ttggagcttt aagatttaat gactgctctg cttggttttg gacttgtgta
721 ggcttgtagc ccctttcttt tggccaattt cttcctttta gaatgggaat gttgatccaa
781 tgcctatacc cccattgtat cttggaagta acaaacttgt ttcttatttt acagggtcat
841 aggcagaagg gactagcctt gtctcagata agactttgga ctgtggactt ttgagttaat
901 gctggaatga gttaaatatt tgcaggactg ttgagaaggc atggtagttt gtttgtttgt
961 ttgtttgttt gtttgtttgt ttgttttttg agacagagtc tccctctgtc accaggctgg
1021 agtgcagtgg tgcgatcgcg gctcactgca atctctgcct cctgggttca gatgattccc
1081 ctgcctcggc ctccccagtg gctggggctg caggcctccg tcaccgtgcc tggctggttt
1141 tttgtgtttt tgtagagatg gggtttcacc ttgttggccg ggatggtctc gatctcctga
1201 ccttctgatc tgcctgcctc ggccacccaa agtgctggga ttacaggcgt gaaccactgc
1261 acgcagccgg tagtattttg aaatatgaga aggacatgag atttgggaga gaccagaggt
1321 ggaacaatat ggtttgaatc tgtgtccccg cccaaatctc atgttgaatt ggaatcccca
1381 gtgttggagg taggacctgg tggaaagtga ttggatcatg gggcggtttc tcattaatgg
1441 tttagcacta tccccttagc gctgttcttg tgagagagtt tgcatgacat ctggttgttt
1501 aaaagtgtgt ggcacctccc cccacacttc ctcctgctcc agccgcgtaa gacatgcctg
1561 tggccgggcg cggtggctca cgcctgtggt cccagcactt tggggggctg aggtgggcgg
1621 atcacgaggt caggagatcg aggccatcgt ggctagcacg gtgaaacccc gtctctacta
1681 ggaatacagg gaattggccg ggcacggtgg cgggcgcctg tggtcccggc tactcgggag
1741 gctgaggcag gagaatggcg tgggcctgga aggcggagct tgcggtgggc cgggatcgcg
1801 ccactgcact tccgcctggg cgaaggagcg agactccatc tcaaaaaaaa aaaaaaaaaa
1861 aaaagcaaag acgtgcctgc tacgctttca ccttccacca tcattgaaag tttcctaagg
1921 cctctccaga agccgaatag ataccagcat catgcttcct gtacagcctt tagaaccatg
1981 agccaattaa acctcttttc tttat
<210>39
<211>3055
<212>DNA
<213>Homo sapiens
<400>39
1 gtttccggga tctggcgggg cctttgtctt gtgctccagc tggagctttg gtcccgtatt
61 ctcggctatt tatccccagc tgcgggaggc cctggtgact ctgctgctgc agtgtctggt
121 ttccctgtga cctgcaggta ctgggagtta catagctaag atgccaggac accccgaaag
181 ccggaaaacg agacgcattc tcactatgtt gcccaggctg gtcttgaact ccttccctca
241 agtgattctt ctgcctaatc ctcccaaagt tcttagatta ctcatgaatg gattggtgtt
301 cttaccactg cagttagtgt gttctctgtt agttcatgtg aaagctggtt gttaaaaaca
361 aaaacaaaca aacaaaaaac tgatattcct cttgctttgt cttttgccat gatacccctt
421 tgccttttat tatgcataaa agcttcctga ggcctcatca gaagttaagc agatgttgat
481 gtcatgcttg tatagccaac agaactgagg ctaataaacc tcttttctgt attaatttgc
541 cagtgttaag tattcccttc tagcagcaca aaatggacta acatcccata ggttatattt
601 ttattttttt cttttgtaaa tactggacaa tgatactctt atataatctt cagtataacc
661 attataattg cccactataa aggagtataa tgaaaggcca tgtattttga atcacgaatg
721 actatttaca aaatgtcata ctacgttttt ctttgagcct gtgacccctc tggcctgtaa
781 aaacacatac atacatttag ttttgattaa catggagtta attttaagtc tgtcactcta
841 aacataagtg ttagctttaa gagaattaca gagcatgtaa ttgtgtttga gtgtaggtgt
901 gtactttttt gagaagaaaa caaaaatatt ggaacaaaat ataatttaaa aagtattggt
961 aatttactag caaactagga atctcaaaga ttctgaaagt aaatatttat tctctgcttt
1021 gtattgaatt tacttctgta aaatcttatg gctgctggct cagaatcttc tcatgcaaat
1081 cctgtttttt tcttgtctgt tgatcatact aaacctaatg tatagttttc tcattccaaa
1141 gttcatgaaa ttttctctat attcctgaga aattctaaga ataactttca taaaatgtat
1201 tgatgttcac aagataattt taagatgtaa ttctacaata tgtgtgttac tacattttat
1261 ttaattagta tattttattt ttgtatttca attggagaac cctattttgg ccaattttta
1321 cttggttttt gtttccattt taatatttta cataattgaa tttttttcac tttattgagc
1381 cattttgttt agatgtacac tgggaaggct tcataattca caaagttgtt ttgacatata
1441 aatgaggtga acaaacacag gaaagtgcta ggtttcctgg gggtataata gatgctctat
1501 aattagcagt aaatattctt gtttaagtca ctttttggct acaagtaaga gattagaaat
1561 attcagcata aagagatggc attaactctg catgtaaaga aaacacatgt atacccaggc
1621 tgtacaactg cctctgaaat taagaggaat tgtgtttatt tcatagttat ctctggtact
1681 tcactgttat tttattttca cccccaacta tgcgtcaatc atagcccttt cccttctttg
1741 cttattatgg ctacagattt ctcactgttc tctgcagccc atgtaatttc atacaatatt
1801 ttgtaggttg tgataaggaa tttgaaattt ttaatatagt gaaaaattgg atttaactgg
1861 agagtttgag gacattttta agttagaaat gcaccatttg aatcatttaa ttggcataat
1921 tgggatgtac tctacacagt ttataggatt aaattactga gttagtaatg tttgtatgaa
1981 agaaatgtta ttagattcta ccacaaaata tagtaaacat gaaatcagtt agaataggta
2041 aagcattaat aatggtaggc tatatatcat aatcactata taaagaaaca tcagaatttg
2101 gaaacccctt aagcaaaaat atatcttaga aatcttaagg acacttaatg gcaagcaaaa
2161 aagtttaaat ttagtttttt ataaattaca tatagcacaa tttatgtgta catgtagaat
2221 cttttagttt ttagtgttta tgtaatatat tagacaataa taaacattta catttatgtt
2281 tacatttata tttaattttt caaatttaat ttaaatttct aaatgcttct gtggatttaa
2341 attttggaat aatcttttct tccccattga tactggaatc ttgggaagtt tcttactcat
2401 atcacaatat gttttattta aatggatgca gcttccattg cagagtttat ataaataatc
2461 acataccaag ccaattcctt agtcattttc tgtattaaaa aactcagaga tctagagagc
2521 ttttggattg aaatatttcc ttggtgattt tggatgcaaa cctgatttct gtgttcacac
2581 catggccaga gatgggactg ttagcaccac ctgcttcttt aatgtcgtcc atatgatcag
2641 catcagcacc agaaactcca ggtgtcccaa acttaaagtg aaagccatag agtcctttgg
2701 tgactcccat gttctgtgct ggttctagaa catgctggta aatatcagag tttctatgct
2761 tatggctgat aagtagacat ttttaaaaat cataaaaact gtattttcta tatagttcag
2821 ctaagtttat gaaaaagatg cagactcagt atttggagca ttgctatcgc tccatgatgt
2881 gtttaaaaat tatttttaaa ttgacaaaat tttatatcta catatatata tatcttgtaa
2941 cacatgatgt ttgaaatgct taattctagc caattaaaaa tgctttacct caatagttat
3001 ttttattgtg agaaaggtca acatggcctt ggcccttaaa aaaaaaaaaa aaaaa
<210>40
<211>1909
<212>DNA
<213>Homo sapiens
<400>40
1 tctaagatga cctctactgg gatgacaggg gtgagttgac aaagcttcgc acatcattca
61 tcctccagca gtctcaccca ggcatgattt catgggagtg gcagaggagc gagggtgctt
121 ttgcagaaag gtacaagagc tttttcaggt cttggcttgc atcacatcct tatccaatga
181 accaaagcaa gtgacatggc tgagagtccc tgtaggaggt gcagggagtg tggggactct
241 acaaggtgac atgtcaaaag gcaggaatac agggaacagt aaagcactgg gactgttatt
301 acaaccagta acttgtttct agaagtctgc ttgatgaaac cttcaagcca catggaggag
361 tcctcaaaga tgtgcttgag atattcatat aatttcttct gctctaagaa ccatgaggtg
421 aacttgacct caaagtgaga ccttgacatt ggggcattta agtcagtatc tgtgtcttcc
481 accccacacc tgatccctga cccaaagtct ccacagagac taatagaacc catcattaga
541 ggaatgggga cctactgcac tcttcgtagt gccagggttg gatgtgttgc ttctcctaag
601 ctgtttccct gcagggcagc acagcactgc ctcggaactc aaggttagcc tcgtgcctct
661 cggccgaatc tgttactcaa ggattcctga aaggcagtct gcctagaaat ggaggtcaaa
721 tgaaagaaag gcctaagggt tttttttgtc ttgttttgtt ttgggttttt tttgagacag
781 agtctcgctc tgtcaccagg ctggagtgca gtggcacgat cttggctcac tgcaacctct
841 gcctcccagg ttcaagcgat tctcctgcct cagcctccca aagtgctggg actgcaggca
901 tgagccactg tgcctgacct tacagcagta ttttttaaaa atcaaaatta atgcaaaaat
961 ccatgatgag gccaggcttg gtggctcatg cctgtggttc cagcactttg ggaggatccc
1021 ttgagcccag gtgtttgaga ccagtttggg taacatggga agagcttgtc tctacaaaca
1081 aaaatttaaa aacaatgagc tgggcatgga actcacacct ctagccccag ctactcaggc
1141 tgaggtggga ggatggcttg agcccaggag ttcagggatg cagtgtgctt tgattgcact
1201 actgcgcttc accctgggca acagagtgag accccatctc tttaaaaaaa aaaaaaaatc
1261 catgatgaac aaaacaagta tttgtttgag acggggtctc gctctgtcgc ccgggctgga
1321 gtgcagtggt gccatctcag ctcactgcaa cctccgcctt ctgggttcaa gcgactctcc
1381 tgcctcagcc tcccgagtag ctgggattac aggagcctgg cactatgcct ggctaatttt
1441 tgtattttta ttggagacac catgttggtc gtggctggtc tccaactcct gacctcgggt
1501 gatccgcgcc ccgcggcctc cgaaggtgct gggattgcag gcgtgagcca ccgtgcccgg
1561 cctaaataaa attttgaaga ggctagaacc ccgcacttgt gccttgagct tactgacctc
1621 aacaccctgg ttccactaaa actttattta caaaattatg ctgccggtct gcaggatgta
1681 gaaaacaatt tgattatttt ggatattaaa atatttttta agtcttgaaa atattgttta
1741 ctattaccaa agtttttgaa ccttcttaaa ttctgtacca gaggtgagtg ccacacccta
1801 acctagtctg agtttttatg tgtaaagatc ataaaacgtt gtaagttttc taaaacacat
1861 aagctctcaa taaacgttag cttatcattg tcaaaaaaaa aaaaaaaaa
Claims (3)
1.一类在人类肝脏中表达的相关基因,其特征在于,它包括:
(a)SEQ ID No.1~SEQ ID No.40所示的序列;
(b)SEQ ID No.1~SEQ ID No.40所示的序列中每条序列的互补序列;
(c)与SEQ ID No.1~SEQ ID No.40所示的序列中每条序列有至少70%同源性的序列;
(d)上述(a)~(c)中一条或数条的组合。
2.如权利要求1所述的相关基因,其特征在于,所述序列包括具有SEQ ID No.1~SEQ ID No.40所示的序列。
3.一种探针分子,其特征在于,所述的探针分子含有权利要求1中所述的序列中约8-100个连续的核苷酸。
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