The specific embodiment:
(1) Ganoderma 10~30wt% (example 10%, 20%, 30%), Semen Ziziphi Spinosae 20~40wt% (example 20%, 30%, 40%), Folium Ginkgo 5~15wt% (example 5%, 10%, 15%) are cleaned, the oven dry back is standby;
(2) above-mentioned Ganoderma, Semen Ziziphi Spinosae, Folium Ginkgo are decocted with water 2 times, little boiling (normal pressure, 100 ℃) for the first time adds 10 times of decoctings boils, and adds 8 times of water gagings the 2nd time to decoct, and each 2 hours, precipitation was filtered, and merging filtrate is concentrated into proportion and is 1.40 extractum;
(3) extractum that step (2) is obtained drying under reduced pressure under 0.08Mpa, 80 ℃ of conditions becomes dry extract, pulverizes 80~100 mesh sieves (example 80,90,100 orders), dry extract;
(4) with dry extract and Sporoderm-broken Ganoderma Lucidum Spore powder 30~50wt% (example 30%, 40%, 50%) with trough type mixing machine fully stir, mixing, mixed powder; Add food stage 85% ethanol again, addition is controlled at 10~30% (examples 10%, 20%, 30%) of mixed powder weight, makes soft material; After 16 order stainless steel sifts, granulation, sterilization (105 ℃, 30 minutes), dry (70 ℃, 240 minutes), filled capsules gets product.Above-mentioned raw materials Ganoderma, Semen Ziziphi Spinosae, Folium Ginkgo, Sporoderm-broken Ganoderma Lucidum Spore powder consumption sum are 100%.Product is applied in preparation to be improved the neutralization of sleep goods and is applied in preparation and improves in the immunity goods.
Product of the present invention (have another name called: MEIBAO dormancy LINGZHI JIAONANG) improve the sleep function laboratory report:
1 material
1.1 sample: MEIBAO dormancy LINGZHI JIAONANG, content is a brown ceramic powder, is provided by Jiangsu Anhui Biology Science Co., Ltd.Sample is the 0.5g/ grain, preservation condition for sealing, put shady and cool dry place, storage life is 24 months, the human body recommended intake is 4500mg/ people/d, in this test by 75mg/kg/d.
1.2 animal: select 120 of Healthy female ICR kind mices for use, provide by Shanghai Slac Experimental Animal Co., Ltd., body weight 18~22g, the quality certification number: SCXK (Shanghai) 2004-0005 number is divided into 12 groups at random by body weight, 10 every group.Environment card number: SYXK (Soviet Union) 2002-0004; Animal feed source and card number: Jiangpu development laboratory animal feed factory, No. 95001, moving (raising) word of Soviet Union.
1.3 instrument: electronic scale, electronic balance, stopwatch.
1.4 reagent: pentobarbital sodium, barbital sodium is analytical pure.
2. method
2.1 dosage and grouping: the used animal of every experiment all is divided into 4 groups at random by body weight, every group 10, establish 375,750,2250mg/kg b.wt../d (be equivalent to respectively be grown up per kg body weight per day recommended intake 5 times, 10 times and 30 times) three sample dose and negative control group (distilled water).
2.2 tried giving of thing: take by weighing sample 3750,7500,22500mg, adding distilled water respectively is mixed with each group to 200ml and is subjected to test solution (per two days preparation once), per os is irritated stomach and is given mice, and negative control group gives distilled water, irritates the stomach volume and is 20ml/kg b.wt...All animals all gives the Mus full-valence pellet feed and freely eats, continuous 30 days.Wherein observed direct sleep effect in 29 days in irritating stomach for one group.
2.3 tried the influence of thing to the weight of animals: each treated animal is weighed in before and after test.
2.4 directly sleep experiments and the experiment length of one's sleep of prolongation pentobarbital sodium:
2.4.1 direct sleep experiments: each dosage treated animal gives correspondingly to be tried thing, control animals gives with behind the volume distilled water, with the righting reflex loss is whether the index observing animal phenomenon of sleeping occurs, with righting reflex loss with revert to the digit synbol typing and sleep the animal sleep time.
2.4.2 prolong the pentobarbital sodium experiment length of one's sleep: each treated animal is all irritated stomach 15min pneumoretroperitoneum injection pentobarbital sodium 55mg/kg in last, injection volume is 0.2ml/20g, can with the mice righting reflex loss be index, observe given the test agent and prolong pentobarbital sodium length of one's sleep.
2.5 barbital sodium sub-threshold dose hypnosis experiment: each treated animal is all irritated stomach 15min pneumoretroperitoneum in last and is annotated barbital sodium 135mg/kg, injection volume is 0.2ml/20g, with the mice righting reflex loss is sleeping index more than 1 minute, is recorded in the sleeping mice number of each group in 30 minutes.
2.6 barbital sodium sleep experiment incubation period: each treated animal is all irritated stomach 15min pneumoretroperitoneum injection barbital sodium 215mg/kg in last, and injection volume is 0.2ml/20g, is index with the mice righting reflex loss, writes down each treated animal and sleeps incubation period.
2.7 statistical method: use the SPSS statistical software.All measurement datas are carried out normal state check and homogeneity test of variance earlier, all satisfy the neat requirement of normal distribution and variance, carry out statistical disposition with the comparative approach in twos of mean between one factor analysis of variance method and a plurality of experimental group and matched group.Enumeration datas such as sleeping number are analyzed with X 2 test.
3. result:
3.1 MEIBAO dormancy LINGZHI JIAONANG sees Table 1 to the influence of mice body weight.
Table 1 MEIBAO dormancy LINGZHI JIAONANG to the influence of mice body weight (
)
Prolong the pentobarbital sodium experimental group length of one's sleep
Dosage group (mg/kg) |
Number of animals (only) |
Initial body weight (g) |
P value * |
End-body heavy (g) |
P value * |
0 375 750 2250 |
10 10 10 10 |
20.2±0.8 19.9±0.6 20.2±0.8 19.9±0.9 |
-- >0.05 >0.05 >0.05 |
26.6±1.1 26.3±1.3 25.9±1.4 26.4±1.3 |
-- >0.05 >0.05 >0.05 |
Barbital sodium sub-threshold dose hypnosis experimental group
Dosage group (mg/kg) |
Number of animals (only) |
Initial body weight (g) |
P value * |
End-body heavy (g) |
P value * |
0 375 750 2250 |
10 10 10 10 |
19.6±1.1 20.0±0.7 19.9±1.3 19.8±1.0 |
-- >0.05 >0.05 >0.05 |
25.9±1.5 26.2±1.0 25.9±1.2 26.8±1.5 |
-- >0.05 >0.05 >0.05 |
Barbital sodium sleep experimental group incubation period
Dosage group (mg/kg) |
Number of animals (only) |
Initial body weight (g) |
P value * |
End-body heavy (g) |
P value * |
0 375 750 2250 |
10 10 10 10 |
20.0±0.8 20.1±0.8 20.1±0.8 19.9±0.8 |
-- >0.05 >0.05 >0.05 |
26.4±1.2 26.7±1.2 27.1±1.0 26.5±1.2 |
-- >0.05 >0.05 >0.05 |
*: one factor analysis of variance has the p value of zero difference between four groups
By table 1 as seen, difference (P>0.05) that there are no significant between the initial body weight group of each experimental project mice and between the reorganization of whole opisthosoma, this sample does not have obvious influence to weight of mice.
3.2 MEIBAO dormancy LINGZHI JIAONANG sees Table 2 to the direct sleep effect of mice.
Table 2 MEIBAO dormancy LINGZHI JIAONANG is to the direct sleep effect of mice
By table 2 as seen, the sleep phenomenon appearred in equal end after each organized mouse stomach, and MEIBAO dormancy LINGZHI JIAONANG does not have direct sleep effect, this experimental result feminine gender to mice.
3.3 prolonging the effect of mice pentobarbital sodium length of one's sleep, MEIBAO dormancy LINGZHI JIAONANG sees Table 3.
Table 3 MEIBAO dormancy LINGZHI JIAONANG prolongs the effect of the length of one's sleep of mice pentobarbital sodium
By table 3 as seen, middle and high dosage group mouse sleep time prolongs, and has compared statistical significant difference (p<0.05, p<0.01) with the solvent control group, shows that middle and high dosage group sample can prolong mice pentobarbital sodium length of one's sleep, the experimental result positive.
3.4 MEIBAO dormancy LINGZHI JIAONANG sees Table 4 to the influence of barbital sodium sub-threshold dose syngignoscism.
Table 4 MEIBAO dormancy LINGZHI JIAONANG is to the influence of barbital sodium sub-threshold dose syngignoscism
Dosage group (mg/kg/d) |
Number of animals (only) |
Sleeping number (only) |
Sleeping rate (%) |
The P value |
0 375 750 2250 |
10 10 10 10 |
3 8 10 10 |
30 80 100 100 |
-- 0.035 0.002 0.002 |
By table 4 as seen, through X 2 test, the sleeping mice number of each dosage group is compared with the solvent control group all has statistical significant difference (p<0.05, p<0.01), show that each dosage group sample all has the mice of promotion barbital sodium sub-threshold dose syngignoscism, the experimental result positive.
The preclinical influence of sleeping sees Table 5 3.5 MEIBAO dormancy LINGZHI JIAONANG is to barbital sodium.
Table 5 MEIBAO dormancy LINGZHI JIAONANG is to the barbital sodium preclinical influence of sleeping
*: one factor analysis of variance has the p value of zero difference between four groups
By table 5 as seen, each dosage group mice sleep shortens incubation period, but compares no statistical significant difference (p>0.05), experimental result feminine gender with the solvent control group.
Brief summary: the direct sleep experiments of this sample is feminine gender as a result, prolong the pentobarbital sodium experiment length of one's sleep and the barbital sodium subliminal hypnosis experimental result positive, barbital sodium sleep incubation period is feminine gender as a result, and according to health food function assessment evaluation criterion, this sample has the sleep function of improvement.
MEIBAO dormancy LINGZHI JIAONANG enhancing immunity function zoopery report
1 material and method
1.1 sample: MEIBAO dormancy LINGZHI JIAONANG, content is a brown ceramic powder, is provided by Jiangsu Anhui Biology Science Co., Ltd.Sample is the 0.5g/ grain, and preservation condition is sealing, puts shady and cool dry place, storage life 24 months, the human body recommended intake is the 4.5g/ day for human beings, in this test by 75mg/kg b.wt./d.
1.2 laboratory animal: select 18~22g Healthy female secondary ICR mice that Nanjing medical instruments factory of Zhongmu Industry Co.,Ltd provides (animal card number: SCXK (Soviet Union) 2002-0030 number) for use, respectively be divided into 4 groups at random by body weight respectively, every group 10, be divided into 5 batches and carry out mouse spleen lymphocyte conversion test and NK cytoactive mensuration respectively; The tardy paraphilia reaction of dinitrofluorobenzene inducing mouse (DTH) test; Antibody-producting cell detects and serum hemolysin is measured; The clearance test of mice carbon; Turnover of Mouse Peritoneal Macrophages is engulfed the chicken red blood cell test.Environment card number: SYXK (Soviet Union) 2002-0004; Animal feed source and card number: Jiangpu development laboratory animal feed factory, No. 95001, moving (raising) word of Soviet Union.
1.3 dosage design: adult's (by the 60kg weighing machine) recommended intake every day is 4500mg, be equivalent to 75mg/kg b.wt./d, 10 times of human body recommended amounts are established in experiment, be every day 750mg/kg b.wt. as in the dosage group, upper and lower each 1 dosage group: 2250mg/kg b.wt./d and 375mg/kg b.wt./d are as high dose group and low dose group (be equivalent to respectively human body recommended amounts 30 times and 5 times).Take by weighing sample 22500,7500,3750mg, add respectively distilled water to 200ml be mixed with each the group be subjected to test solution (to deposit in 4 ℃ of refrigerators, preparation in per two days once), per os is irritated stomach and is given mice, irritating the stomach volume is 20ml/kg b.wt., negative control group is irritated distilled water, surveys every immune indexes behind the continuous irrigation stomach 30d.
1.4 key instrument and reagent: electronic scale, electronic balance, microscope, microplate reader, CO2 gas incubator, card punch, 723 spectrophotometers, superclean bench, agitator, constant water bath box, timer, chroma tank, adjustable pipette, test tube, trace blood pipe, 200 eye mesh screens, 24 well culture plates, 96 well culture plates, operating theater instruments etc.
RPMI1640 cell culture fluid, YAC-1 cell, calf serum, 2 mercapto ethanol (2-ME), penicillin, streptomycin, concanavalin A, Con A (ConA), hydrochloric acid, isopropyl alcohol, MTT, Hank ' s liquid, PBS buffer (pH7.2-7.4), 2,4-dinitrofluorobenzene (DNCB), acetone, Oleum Sesami, sheep red blood cell (SRBC) (SRBC), complement (guinea pig serum), SA buffer, Dou Shi reagent, india ink, Na
2CO
3, EINECS 212-761-8, nitro tetrazolium chloride (INT), azophenlyene dimethyl ester sulfate (PMS), NAD, 0.2mol/L Tris-HCl buffer (pH8.2), 2.5%Triton etc.
1.5 experimental technique:
1.5.1 the tardy paraphilia reaction of dinitrofluorobenzene inducing mouse (DTH)
Behind the continuous irrigation stomach 25d, the mouse web portion unhairing, 1% dinitrofluorobenzene (DNFB) acetone Oleum Sesami solution 50ul smears sensitization.5d after the sensitization, mouse right ear evenly smear 1%DNFB acetone Oleum Sesami solution 10ul and attack, and left ear is smeared acetone Oleum Sesami solution and compared, attack back 24h and put to death mice, cut left and right sides auricular concha, weigh in the auricle of same position cut-off footpath 8mm, the difference of left and right sides auricle weight is as the swelling degree.Get thymus and the spleen of mice simultaneously, with the spleen of every 10g mice heavy (mg) and thymus weight (mg) as spleen/body weight ratio and thymus/body weight ratio.
1.5.2ConA inductive mouse spleen lymphocyte transformation experiment (mtt assay)
Behind the continuous irrigation stomach 30d, the aseptic spleen of getting, (cell concentration is 3 * 10 to make the individual cells suspension
6Individual/ml).Divide two holes to add in 24 well culture plates cell suspension, every hole 1ml, a hole adds 75ulConA liquid, and 5%CO is put in contrast in another hole
2, 37 ℃ of CO
2Cultivate 72h in the incubator.Cultivate and finish preceding 4h adding MTT (5mg/mL) 50ul/ hole, continue to cultivate.After cultivating end, every hole adds 1mL acid isopropyl alcohol, and the piping and druming mixing dissolves purple crystal fully.Divide then to install in 96 well culture plates, enzyme-linked immunosorbent assay instrument as parallel sample, is used in each hole packing 3 hole (200ul/ hole), measures optical density value with the 570nm wavelength.Deduct the optical density value that does not add the ConA hole with the optical density value that adds the ConA hole and represent lymphocytic multiplication capacity.
1.5.3 antibody-producting cell detects (PFC)
Behind the continuous irrigation stomach 25d, every Mus lumbar injection 2% (v/v) hematocrit SRBC 0.2ml sensitization.Behind the immunity 5d, the dislocation of mice cervical vertebra is put to death, take out spleen, make single cell suspension, carry out hemolytic plaque test.Counting hemolysis plaque number is with plaque number/10
6Splenocyte is represented the antibody-producting cell number.
1.5.4 serum hemolysin is measured: half hemolysis value (HC
50) algoscopy
Behind the continuous irrigation stomach 25d, every Mus lumbar injection 2% (v/v) hematocrit SRBC 0.2ml sensitization.Behind the immunity 5d, mice is plucked eyeball blood sampling, gets serum and surveys hemolytic reaction, and other establishes not that the control tube of increase serum (replacing with the SA buffer) is the colorimetric blank, measures respectively in 540nm and respectively manages optical density value.Be calculated as follows the sample HC of each Mus
50:
1.5.5 mice carbon clearance test
The india ink 0.1ml of the every 10g body weight of mice tail vein injection 1: 3 (v/v) dilution behind the continuous irrigation stomach 30d, timing immediately after the injection, and respectively at 2,10min gets blood 20 μ l from the angular vein clump, is added in 2ml 0.1% sodium carbonate, surveys OD
600nmAnd get liver, spleen is weighed, by formula
Ask mice phagocytic index a value (K=(lgOD
1-lgOD
2)/(t
2-t
1)).
1.5.6 Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell experiment (dripping the sheet method)
Behind the continuous irrigation stomach 26d, every Mus lumbar injection 2% (v/v) hematocrit SRBC 0.2ml activates mouse macrophage.Behind the 4d, the cervical vertebra dislocation method is put to death mice, and lumbar injection adds Hank ' s liquid 4mL/ of calf serum, draws 1% chicken red blood cell mixing of abdominal cavity washing liquid and equivalent.Draw the 0.5mL mixed liquor, add in the agar circle of slide, place incubator and hatched 20 minutes for interior 37 ℃.Hatch finish the back with normal saline not attached cell wash out, in methanol solution, fix 1 minute, Giemsa liquid dyeing 15 minutes.(phagocytic rate is in per 100 macrophages, engulfs the shared percentage rate of macrophage of chicken red blood cell with 40 * microscopic counting phagocytic rate and phagocytic index; Phagocytic index is the number of average each macrophage phagocytic chicken red blood cell).And judge the phagocytic activity of macrophage in view of the above.
1.5.7NK cytoactive is measured
Behind the continuous irrigation stomach 30d, the aseptic spleen of getting, (cell concentration is 2 * 10 to make the individual cells suspension
7Individual/mL), the action effect cell.Get target cell (YAC-1 cell) and each 100ul of effector lymphocyte (imitating target), add in U type 96 well culture plates than 50: 1; Target cell nature release aperture adds target cell and each 100ul of culture fluid, and the maximum release aperture of target cell adds target cell and each 100ul of 2.5%Triton; Above-mentioned every three repeating holes of all establishing are in 37 ℃, 5%CO
2Cultivate 4h in the incubator, then with 96 well culture plates with the centrifugal 5min of 1500r/min, draw at the bottom of the supernatant 100ul horizontalization in 96 well culture plates in every hole, add LDH substrate liquid 100ul simultaneously, reaction 3min, every hole adds the HCl 30ul of 1mol/L, measures optical density value (OD) at microplate reader 490nm place.
Be calculated as follows the NK cytoactive:
1.6 experimental data statistics:
This experiment gained mice body weight, dirty body ratio, optical density value, swelling degree, hemolysis plaque number, HC
50, data such as phagocytic rate and phagocytic index, mice phagocytic index a, NK cytoactive are measurement data, initial data (wherein phagocytic rate is behind square arcsine transformation) adopts the SPSS statistical software to carry out test of normality and homogeneity test of variance, satisfy " variance is neat " and " normal distribution " requirement, reuse SPSS statistical software one factor analysis of variance method is added up, and the data of P<0.05 are carried out statistical disposition with the comparative approach in twos of mean between a plurality of experimental grouies and matched group.
2 experimental results
2.1 sample is to the influence of the weight of animals before and after the experiment
By table 1, table 2, as seen table 3 is respectively tried the weightening finish of agent amount group mice and is compared there was no significant difference (P>0.05) with matched group before and after the experiment.
Table 1 respectively organize mice initial body weight (
)
Group |
Drench the active group of commentaries on classics/NK
|
Tardy paraphilia reaction group
|
PFC/HC
50Group
|
Body weight (g) |
The P value |
Body weight (g) |
The P value |
Body weight (g) |
The P value |
Dosage group high dose group in the matched group low dose group |
20.1±0.7 20.0±1.0 20.2±1.1 20.2±1.0 |
--- >0.05 >0.05 >0.05 |
20.8±1.3 20.5±1.2 20.9±1.0 20.4±1.0 |
--- >0.05 >0.05 >0.05 |
20.4±1.2 20.2±1.2 20.3±1.3 20.2±1.3 |
--- >0.05 >0.05 >0.05 |
Table 1 (continuing) respectively organize mice initial body weight (
)
Group |
Peritoneal macrophage is engulfed group
|
Carbon is cleaned up group
|
Body weight (g) |
The P value |
Body weight (g |
The P value |
Dosage group high dose group in the matched group low dose group |
19.7±1.0 19.9±0.9 19.8±1.2 19.6±1.1 |
--- >0.05 >0.05 >0.05 |
19.2±0.9 19.3±1.2 19.3±1.0 19.4±1.0 |
--- >0.05 >0.05 >0.05 |
Table 2 test end respectively organize mice body weight (
)
Group |
Drench the active group of commentaries on classics/NK
|
Tardy paraphilia reaction group
|
PFC/HC
50Group
|
Body weight (g) |
The P value |
Body weight (g) |
The P value |
Body weight (g) |
The P value |
Dosage group high dose group in the matched group low dose group |
33.3±2.8 34.6±2.2 34.0±0.8 34.4±2.5 |
--- >0.05 >0.05 >0.05 |
35.2±2.0 33.5±2.7 36.2±2.7 33.8±2.2 |
--- >0.05 >0.05 >0.05 |
33.9±3.0 33.6±1.7 34.3±2.7 34.0±1.7 |
--- >0.05 >0.05 >0.05 |
Table 2 (continuing) test end respectively organize mice body weight (
)
Group |
Peritoneal macrophage is engulfed group
|
Carbon is cleaned up group
|
Body weight (g) |
The P value |
Body weight (g) |
The P value |
Dosage group high dose group in the matched group low dose group |
33.1±1.9 33.1±2.1 33.4±1.5 32.9±1.6 |
--- >0.05 >0.05 >0.05 |
31.4±2.5 32.3±2.3 32.2±2.1 32.5±1.8 |
--- >0.05 >0.05 >0.05 |
Table 3 sample to the influence of mice weight increase (
)
Group |
Drench the active group of commentaries on classics/NK
|
Tardy paraphilia reaction group
|
PFC/HC
50Group
|
Weight increase (g) |
The P value |
Weight increase (g) |
The P value |
Weight increase (g) |
The P value |
Dosage group high dose group in the matched group low dose group |
13.2±2.8 14.5±2.3 13.8±1.4 14.2±2.8 |
--- >0.05 >0.05 >0.05 |
14.4±2.2 13.0±2.9 15.3±2.3 13.4±2.1 |
--- >0.05 >0.05 >0.05 |
13.6±2.9 13.4±2.1 13.9±2.5 13.8±2.4 |
--- >0.05 >0.05 >0.05 |
Table 3 (continuing) sample to the influence of mice weight increase (
)
Group |
Peritoneal macrophage is engulfed group
|
Carbon is cleaned up group
|
Weight increase (g) |
The P value |
Weight increase (g) |
The P value |
Dosage group high dose group in the matched group low dose group |
13.4±2.4 13.2±2.2 13.6±1.8 13.3±1.4 |
--- >0.05 >0.05 >0.05 |
12.2±2.5 12.9±2.9 12.9±1.5 13.1±1.9 |
--- >0.05 >0.05 >0.05 |
2.2 sample is to the influence of internal organs/body weight ratio
By table 4 as seen, each organizes mouse thymus/body ratio, spleen/body ratio is compared there was no significant difference (P>0.05) with matched group.
Table 4 MEIBAO dormancy LINGZHI JIAONANG is to the influence of mice internal organs (mg)/body weight (10g) ratio
2.3 cellular immune function test: the tardy paraphilia reaction of the inductive mice of dinitrofluorobenzene (DNFB) (DTH)
By table 5 as seen, the auricle swelling degree of each dosage group mice all is higher than the solvent control class value, and increases with dosage, and through variance analysis and comparative statistics processing in twos, each dosage group all has statistical significant difference (P<0.05, P<0.01).Prompting MEIBAO dormancy LINGZHI JIAONANG can strengthen the tardy paraphilia reaction of mice that DNFB causes.
Table 5 MEIBAO dormancy LINGZHI JIAONANG is to the influence of the tardy paraphilia reaction of Mice Auricle
2.4 cellular immune function test: the inductive mouse spleen lymphocyte transformation experiment of ConA (mtt assay)
By table 6 as seen, each dosage group optical density difference all is higher than the solvent control class value, but the result reaches comparative statistics processing in twos through variance analysis, and each dosage group does not all have statistical significant difference (P>0.05).Prompting MEIBAO dormancy LINGZHI JIAONANG does not have obvious enhancing mouse lymphocyte multiplication capacity.
The influence that table 6 MEIBAO dormancy LINGZHI JIAONANG transforms mouse spleen lymphocyte
Group |
Take in dosage (mg/kg/d) every day |
Number of animals (only) |
Optical density value
|
Optical density difference |
The P value |
-ConA |
+ConA |
Control sample |
0 375 750 2250 |
10 10 10 10 |
0.164±0.035 0.233±0.070 0.177±0.044 0.189±0.080 |
0.358±0.082 0.438±0.098 0.415±0.071 0.405±0.081 |
0.194±0.066 0.205±0.048 0.238±0.074 0.217±0.058 |
--- 0.690 0.186 0.433 |
2.5 the humoral immune function test: antibody-producting cell detects (Jerne improves slide method)
By table 7 as seen, each dosage group plaque number average is higher than the solvent control class value, and through variance analysis and comparative statistics processing in twos, middle and high dosage group plaque number has been compared significant difference (P<0.05, P<0.01) with the contrast class value.Point out middle and high dosage MEIBAO dormancy LINGZHI JIAONANG to have and strengthen the ability that mice produces antibody-producting cell.
Table 7 MEIBAO dormancy LINGZHI JIAONANG is to the influence of mice spleen antibody-producting cell amount
2.6 humoral immune function test: serum hemolysin test
By table 8 as seen, each dosage group mice serum half hemolysis value (HC
50) and all be higher than the contrast class value, and raise with the dosage increase, through variance analysis and comparative statistics processing in twos, high dose group has statistical significant difference (P<0.05).Prompting high dose MEIBAO dormancy LINGZHI JIAONANG can strengthen the ability that mice produces serum hemolysin.
Table 8 MEIBAO dormancy LINGZHI JIAONANG is to the influence of serum hemolysin level
2.7 monokaryon-macrophage function test: mice carbon clearance test
By table 9 as seen, the phagocytic index a of each dosage group mice all is higher than the solvent control group, and through variance analysis and comparative statistics processing in twos, each dosage group all has statistical significant difference (P<0.05).Prompting MEIBAO dormancy LINGZHI JIAONANG has the effect that enhancing mice carbon is cleaned up ability.
The influence that table 9 MEIBAO dormancy LINGZHI JIAONANG is cleaned up mice carbon (
)
Group |
Take in dosage (mg/kg/d) every day |
Number of animals (only) |
The K value |
Phagocytic index a |
The P value |
Control sample |
0 375 750 2250 |
10 10 10 10 |
0.0406±0.014 0.0462±0.008 0.0457±0.009 0.0465±0.008 |
6.372±0.27 6.744±0.43 6.865±0.55 6.778±0.41 |
--- 0.032 0.020 0.018 |
2.8 monokaryon-macrophage function test: Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell
Phagocytic rate and phagocytic index by visible each the dosage group mice of table 10 all are higher than the solvent control group, and raise with the dosage increase, through variance analysis and comparative statistics processing in twos, to compare with the contrast class value, high dose group phagocytic rate and phagocytic index all have significant difference (P<0.01).Prompting high dose MEIBAO dormancy LINGZHI JIAONANG has the effect that strengthens the Turnover of Mouse Peritoneal Macrophages phagocytic activity.
Table 10 MEIBAO dormancy LINGZHI JIAONANG to the influence of Turnover of Mouse Peritoneal Macrophages phagocytic activity (
)
Group |
Take in dosage (mg/kg/d) every day |
Number of animals (only) |
Phagocytic rate (%) |
The P value |
Phagocytic index |
The P value |
Control sample |
0 375 750 2250 |
10 10 10 10 |
20.8±3.6 21.6±5.4 24.9±6.3 27.7±5.5 |
0.728 0.093 0.003 |
0.259±0.054 0.282±0.085 0.323±0.092 0.359±0.072 |
0.480 0.075 0.003 |
2.9NK cytoactive is measured
NK cytoactive by visible each the dosage group mice of table 11 all is higher than the solvent control group, the result is through variance analysis and comparative statistics processing in twos, middle and high dosage group all has statistical significant difference (P<0.01, P<0.05), shows that middle and high dosage MEIBAO dormancy LINGZHI JIAONANG all has the active effect of the NK cells in mice of enhancing.
Table 11 MEIBAO dormancy LINGZHI JIAONANG to the active influence of NK cells in mice (
)
Group |
Take in dosage (mg/kg/d) every day |
Number of animals (only) |
NK cytoactive (%) |
The P value |
Control sample |
0 375 750 2250 |
10 10 10 10 |
51.0±16.0 67.7±26.4 88.4±25.2 75.8±23.2 |
--- 0.105 0.001 0.012 |
3 brief summaries: per os gives the MEIBAO dormancy LINGZHI JIAONANG 30d of mice various dose, and weight of mice is not had influence; Basic, normal, high dosage MEIBAO dormancy LINGZHI JIAONANG all can strengthen the effect that the tardy paraphilia of mice is reacted and enhancing mice carbon is cleaned up ability that DNFB causes: middle and high dosage MEIBAO dormancy LINGZHI JIAONANG has the ability and the active effect of enhancing NK cells in mice that mice produces antibody-producting cell that strengthen; High dose MEIBAO dormancy LINGZHI JIAONANG has ability that strengthens mice generation serum hemolysin and the effect that strengthens the Turnover of Mouse Peritoneal Macrophages phagocytic activity.According to health food enhancing immunity functional evaluation standard, MEIBAO dormancy LINGZHI JIAONANG has the function of enhancing immunity.