CN1963461A - Lucifugal and radiating structure for testing PCR fluorescence of micro flow control biologic chip - Google Patents
Lucifugal and radiating structure for testing PCR fluorescence of micro flow control biologic chip Download PDFInfo
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- CN1963461A CN1963461A CN 200610114661 CN200610114661A CN1963461A CN 1963461 A CN1963461 A CN 1963461A CN 200610114661 CN200610114661 CN 200610114661 CN 200610114661 A CN200610114661 A CN 200610114661A CN 1963461 A CN1963461 A CN 1963461A
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- airtight container
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Abstract
This invention relates to one light shield dissipation structure used in micro flow control biological chip of PCR fluorescence test, which provides ensured outside devices and fluorescence spectrum test device sealed inside light sealed capacitor for micro flow control biological polymer enzyme linkage reaction, wherein, it plugs n ring tanks through each surface of same shape for gas-in baseboard and light shield gas-in cover board, light shield dissipation baseboard and light shield dissipation cover board.
Description
Technical field
The present invention relates to a kind of lucifuge radiator structure that is used for microflow controlled biochip PCR fluoroscopic examination, be mainly used in and avoid veiling glare in the biological PCR fluorescence detecting system, belong to biology and medical science detection range the interference of fluoroscopic examination and the heat elimination and cooling of fluorescence detecting system.
Background technology
PCR (PCR) is a kind of nucleic acid amplification technologies in in-vitro simulated natural dna replication dna process, it is the asexual cell molecule clone technology, its principle is similar to duplicating of n DNA, is a kind of method that external enzymatic reaction selectivity ground synthesizes specific DNA.Reactions steps is that two terminal sequences of a pair of Oligonucleolide primers of synthetic and two chains of specific amplified dna fragmentation are complementary respectively, form the one-period circulation by high temperature (about 96 degree Celsius) thermal denaturation, low temperature (about 55 degree Celsius) renaturation and thermophilic (about 72 degree Celsius) extension and carry out, make dna fragmentation be able to rapid amplification.Under appropraite condition, this circulation constantly repeats, and previous round-robin product D NA can be used as a back round-robin template DNA and participates in the synthetic of DNA, and the amount that makes product D NA is by 2
nMode increases.Through the reaction of 30 multiple cycles, the DNA cloning multiple is 10 theoretically
6～10
9
The fluorescent quantitative PCR technique that generally uses at present both at home and abroad, transmit technology based on fluorescent energy has: TaqMan technology, Am2 plisensor technology, Molecular beacon technology (molecular beacon), Lightcycler technology and Complex probes technology (combined probe method).By molecular biology theory and technology signature analysis to above-mentioned several PCR quantitative techniques, can make such diagnosis: although the principle method of above-mentioned several PCR quantitative technique on the biotechnology aspect has nothing in common with each other, but their fluorescence radiation mechanism and luminous purpose all are the FRET that destroys between two fluorescence molecules, thereby send fluorescence.Fluorescence intensity is directly proportional with template amount in the solution, according to fluorescence intensity with the information that the variation of fluorescence intensity changes, can carry out qualitative and quantitative analysis to the PCR product.Wherein, the fluorescence spectrum detection technique since its selectivity good, characteristics such as trace qualitative and quantitative analysis and non-destructive detection, become gradually most widely used general, one of detection technique that sensitivity is the highest.
Because pcr amplification needs nearly 100 to carry out thermal denaturation, and the photoelectric component best operating condition in the fluorescence spectrum detection system is normal temperature (about 30 degree Celsius), therefore, how to handle and both avoided veiling glare to the interference of fluoroscopic examination and seal detection system, the while fluorescence detecting system is this problem of heat elimination and cooling again, is the problem that needs fine solution in the design of biological PCR fluorescence detecting system.
At present, existing P CR fluorescence detecting system mainly comprises reaction pipe PCR fluorescence detecting system and micro-fluidic formula PCR fluorescence detecting system.
Reaction pipe PCR fluorescence detecting system its essence is to place special container----at reaction tube reactant, the inner reaction tube thing is carried out elevated temperature heat sex change, process annealing and thermophilic extend amplification cycle of composition.Be that the inner reaction tube thing is in static state, and temperature amplification loop cycle is in dynamically.That is to say, at each constantly, reaction pipe PCR fluorescence detecting system has only a warm area to exist, only the temperature of warm area is under the work of heating and refrigerating plant, change in loop cycle in time, therefore, reaction pipe PCR fluorescence detecting system does not have specific (special) requirements to the efficient of lucifuge radiator structure and the heat radiation of its lucifuge, generally is by electric fan and some structure slit heat extractions.
The micro-fluidic formula PCR of microflow controlled biochip reacts essence, and three warm areas forming an amplification cycle are lain on the chip plane, makes the microchannel on chip, adopts the external valve controls pressure-driven and operates liquid stream.Sample enters the microchannel, and reactant is micro-fluidic following in the microchannel, by flowing on three open and flat warm areas, realizes an amplification loop cycle.Repeatedly circulate the realization loop cycle that repeatedly increases.Be that the inner reaction tube thing is in dynamically, and temperature amplification loop cycle is in static state.That is to say, at each constantly, in the micro-fluidic formula PCR fluorescence detecting system, all there are three open and flat warm areas to exist simultaneously and need maintenance normal working temperature, in order to make above-mentioned three open and flat warm areas keep normal working temperature without interfering with each other, between three warm areas, need the flow at high speed air, form the temperature isolation district between the warm area, therefore, the micro-fluidic formula PCR fluorescence detecting system lucifuge radiator structure and the efficient of microflow controlled biochip are the technical meaning with particular importance in said system.As shown in Figure 6,40 round-robin PCR micro-fluidic chips are made up of for 55 ℃ inlet, outlet, 94 ℃ in sex change district, 72 ℃ of extension areas and annealed zone, and the aperture between adjacent warm area is heat insulation grid, and the overall dimensions of chip is: 40mm * 100mm.
At present, do not have special radiator structure for micro-fluidic formula PCR fluorescence detecting system, in experiment, the interference of veiling glare to detection system avoided in the room that just is placed on lucifuge.Owing to need flow air to come thermal insulation between three warm areas, also just use fan to wait the flowability that increases air in the reality in the room of lucifuge.This just makes the environment for use of micro-fluidic formula PCR fluorescence detecting system be restricted, and because room space is big, for micro-fluidic formula PCR fluorescence detecting system, the effect of thermal insulation neither be very desirable.
Summary of the invention
In order to address the above problem well, provide a kind of lucifuge radiator structure that is used for microflow controlled biochip PCR fluoroscopic examination among the present invention.The present invention can improve the micro-fluidic formula PCR fluorescence detecting system radiating efficiency of microflow controlled biochip, satisfies three open and flat warm areas and has and keep normal working temperature simultaneously, also can avoid the influence of veiling glare to system simultaneously.
In order to reach the purpose of lucifuge heat radiation, the present invention has taked to get following technical scheme.This structure mainly includes lucifuge airtight container 5, lucifuge heat extraction substrate 3, lucifuge heat extraction cover plate 4, lucifuge air inlet substrate 7, lucifuge air inlet cover plate 8.Wherein, the fluorescence spectrum pick-up unit that the peripherals of giving security for the microflow controlled biochip PCR and being used to detects the PCR product is sealed in the lucifuge airtight container (5), in lucifuge airtight container 5, be provided with two fans 1, be provided with air intake opening 6 and exhausr port 2 with the chamber wall of the corresponding lucifuge airtight container 5 of fan, outside surface at lucifuge airtight container 5, the position that is positioned at around air intake opening 6 and the exhausr port 2 is fixed with lucifuge air inlet substrate 7 and lucifuge heat extraction substrate 3, air intake opening 6 respectively, exhausr port 2 is respectively by lucifuge air inlet substrate 7, the through hole that is provided with on the lucifuge heat extraction substrate 3 is in communication with the outside.Lucifuge air inlet substrate 7 and lucifuge air inlet cover plate 8 gone up N identical ring groove of the shape that is provided with to inserting by surface separately, then the two is fixed on the lucifuge airtight container 5, and leaving ventilation gap when slotting.Lucifuge heat extraction substrate 3, lucifuge heat extraction cover plate 4 also by being arranged on lip-deep separately N ring groove to inserting, are fixed on them on the lucifuge airtight container 5 then, and make and leave enough ventilation gaps to inserting the place.In the lucifuge airtight container 5, optical fiber that detection system is required and electrical lead, the through hole that passes on the chamber wall of lucifuge airtight container 5 is used to detect reading of data with extraneous the connection, and does the lucifuge processing in the through hole.
The span of described N is 1～100.
The closed ring pattern that is shaped as different shapes such as circle, ellipse, square, rectangle, polygon of described ring groove.
The size dimension of lucifuge heat extraction substrate 3, lucifuge heat extraction cover plate 4, lucifuge air inlet substrate 7, lucifuge air inlet cover plate 8 can design as the case may be, not necessarily leaves no choice but and lucifuge airtight container 5 the same size and symmetric arrangement.
In lucifuge airtight container 5, optical fiber that detection system is required and electrical lead by the hole of having accomplished fluently on lucifuge airtight container 5 walls, after making lucifuge and handling, are realized that the lucifuge airtight container is inside and outside to connect.
Principle of work of the present invention: when fan 1 energising work, lucifuge airtight container 5 outer cold airs arrive air intake opening 6 along the gap between lucifuge air inlet substrate 7 and 8 pairs of slotting N ring grooves of lucifuge air inlet cover plate, enter in the lucifuge airtight container 5.And the hot-air in the lucifuge airtight container 5 is under the suction and pumping effect of fan 1, through the gas outlet 2,, arrive outside the lucifuge airtight container 5 along lucifuge heat-radiating substrate 3,4 pairs of slotting N ring grooves of lucifuge heat extraction cover plate, realize the 5 inside and outside hot cold air exchanges of lucifuge airtight container, reached the heat extraction effect.Simultaneously, because light press the straight line transmission, and a part of reflection of light and scattering are also intercepted N the ring groove of inserting by multilayer, therefore, have realized that the interior veiling glare of avoiding of lucifuge airtight container 5 seals the detection system effect to the interference of fluoroscopic examination.Simultaneously,, also make the air velocity that is used for thermal insulation between each warm area of fluorescence detecting system accelerate, strengthened the thermal insulation effect between each warm area because the air flow in the airtight container 5 is accelerated.
Through experimental verification, the present invention can be fine some device in the solution lucifuge airtight container 5 under the situation of the normal working temperature that keeps nearly 100, photoelectric component can be in normal temperature (about the 30 degree Celsius) work of best operating condition in the fluorescence spectrum detection system.Simultaneously, whole lucifuge airtight container 5 interior no veiling glares are to the interference of fluoroscopic examination.
Description of drawings
Fig. 1 is used for the lucifuge radiator structure system schematic cross-section of biological PCR fluoroscopic examination
The round closed loop shape synoptic diagram of lucifuge air inlet (or lucifuge heat extraction) the substrate ring groove in Fig. 2 lucifuge radiator structure system
The round closed loop shape synoptic diagram of lucifuge air inlet (or lucifuge heat extraction) the cover plate ring groove in Fig. 3 lucifuge radiator structure system
The square closed loop shape synoptic diagram of lucifuge air inlet (or lucifuge heat extraction) the substrate ring groove in Fig. 4 lucifuge radiator structure system
The square closed loop shape synoptic diagram of lucifuge air inlet (or the lucifuge heat extraction) cover plate in Fig. 5 lucifuge radiator structure system (figure is right) ring groove
0 round-robin PCR of Figure 64 micro-fluidic chip synoptic diagram
Among the figure: 1, fan, 2, the gas outlet, 3, lucifuge heat extraction substrate, 4, lucifuge heat extraction cover plate, 5, lucifuge airtight container, 6, air intake opening, 7, lucifuge air inlet substrate, 8, lucifuge air inlet cover plate, 9, transversal is the ring groove projection of rectangle, 10, biological PCR reagent injection port, 11, the microchannel of micro-fluidic chip, 12, air-cooled thermal insulation hole, 13, biological PCR reagent outlet.
Embodiment
Embodiment 1:
Describe present embodiment in detail below in conjunction with Fig. 1, Fig. 2, Fig. 3.
Present embodiment mainly comprises fan 1, gas outlet 2, lucifuge heat extraction substrate 3, lucifuge heat extraction cover plate 4, lucifuge airtight container 5, air intake opening 6, lucifuge air inlet substrate 7, lucifuge air inlet cover plate 8.Lucifuge air inlet substrate 7 and lucifuge air inlet cover plate 8 gone up N identical ring groove of the shape that is provided with to after inserting by surface separately, uses screw bolt and nut, they is separately fixed on the lucifuge airtight container 5, and makes and locate to leave enough ventilation gaps to inserting.Lucifuge heat extraction substrate 3, lucifuge heat extraction cover plate 4 also by being arranged on lip-deep separately N ring groove to after inserting, also use screw bolt and nut, they are separately fixed on the lucifuge airtight container 5, and make and leave enough ventilation gaps to inserting the place.
The fluorescence spectrum pick-up unit that the PCR product is detected in PCR and being used to is sealed in the lucifuge airtight container 5, fan is arranged in the lucifuge airtight container 5, in the lucifuge airtight container 5, optical fiber that detection system is required and electrical lead, by having openning hole on lucifuge airtight container 5 walls, and after doing the lucifuge processing, realize the inside and outside connection of lucifuge airtight container.Fill with filling material in gap between electrical lead and hole, reaches the purpose of lucifuge.
Lucifuge heat-radiating substrate 3, lucifuge heat extraction cover plate 4, lucifuge air inlet substrate 7 and lucifuge air inlet cover plate 8 all are the plates that have 5 annular grooves, wherein the pattern of annular groove such as Fig. 2, shown in Figure 3.
5 annular grooves of lucifuge heat-radiating substrate 3 and lucifuge heat extraction cover plate 4, lucifuge air inlet substrate 7 and lucifuge air inlet cover plate 8 can be to inserting, and leaving enough ventilation gaps when inserting, as shown in Figure 1.
Embodiment 2:
Describe present embodiment in detail below in conjunction with Fig. 1, Fig. 4, Fig. 5.
Present embodiment mainly comprises fan 1, gas outlet 2, lucifuge heat extraction substrate 3, lucifuge heat extraction cover plate 4, lucifuge airtight container 5, air intake opening 6, lucifuge air inlet substrate 7, lucifuge air inlet cover plate 8.Lucifuge air inlet substrate 7 and lucifuge air inlet cover plate 8 gone up N identical ring groove of the shape that is provided with to after inserting by surface separately, uses screw bolt and nut, they is separately fixed on the lucifuge airtight container 5, and makes and locate to leave enough ventilation gaps to inserting.Lucifuge heat extraction substrate 3, lucifuge heat extraction cover plate 4 also by being arranged on lip-deep separately N ring groove to after inserting, also use screw bolt and nut, they are separately fixed on the lucifuge airtight container 5, and make and leave enough ventilation gaps to inserting the place.
The fluorescence spectrum pick-up unit that the PCR product is detected in PCR and being used to is sealed in the lucifuge airtight container 5, fan is arranged in the lucifuge airtight container 5, in the lucifuge airtight container 5, optical fiber that detection system is required and electrical lead, by having openning hole on lucifuge airtight container 5 walls, and after doing the lucifuge processing, realize the inside and outside connection of lucifuge airtight container.
Lucifuge heat-radiating substrate 3, lucifuge heat extraction cover plate 4, lucifuge air inlet substrate 7 and lucifuge air inlet cover plate 8 all are the plates that have 5 square annular grooves, its square annular groove pattern such as Fig. 4, shown in Figure 5.5 square annular grooves of lucifuge heat-radiating substrate 3 and lucifuge heat extraction cover plate 4, lucifuge air inlet substrate 7 and lucifuge air inlet cover plate 8 can be to inserting, and leaving enough ventilation gaps when inserting, as shown in Figure 1.
Claims (3)
1, is used for the lucifuge radiator structure of microflow controlled biochip PCR fluoroscopic examination, it is characterized in that: mainly include lucifuge airtight container (5), lucifuge heat extraction substrate (3), lucifuge heat extraction cover plate (4), lucifuge air inlet substrate (7), lucifuge air inlet cover plate (8); Wherein, the fluorescence spectrum pick-up unit that the peripherals of giving security for the microflow controlled biochip PCR and being used to detects the PCR product is sealed in the lucifuge airtight container (5), optical fiber and electrical lead that detection system in lucifuge airtight container (5) is required, the through hole that passes on the chamber wall of lucifuge airtight container (5) is used to detect reading of data with extraneous the connection; In lucifuge airtight container (5), be provided with two fans (1), be provided with air intake opening (6) and exhausr port (2) with the chamber wall of the corresponding lucifuge airtight container of fan (5), at the outside surface of lucifuge airtight container (5), be positioned at air intake opening (6) and exhausr port (2) position on every side is fixed with lucifuge air inlet substrate (7) and lucifuge heat extraction substrate (3) respectively, air intake opening (6), exhausr port (2) are gone up the through hole that is provided with and are in communication with the outside by lucifuge air inlet substrate (7), lucifuge heat extraction substrate (3) respectively; Lucifuge air inlet substrate (7) passes through N identical ring groove of the surperficial separately shape that upward is provided with to inserting with lucifuge air inlet cover plate (8), when inserting, between the two ring groove that is in contact with one another, leave ventilation gap, then the two is fixed on the lucifuge airtight container (5); Lucifuge heat extraction substrate (3), lucifuge heat extraction cover plate (4) also by being arranged on lip-deep separately N ring groove to inserting, leave enough ventilation gaps to inserting the place, also the two are fixed on the lucifuge airtight container (5) then.
2, the lucifuge radiator structure that is used for the biological PCR fluoroscopic examination according to claim 1, it is characterized in that: the span of described N is 1～100.
3, the lucifuge radiator structure that is used for the biological PCR fluoroscopic examination according to claim 1 is characterized in that: described ring groove is the closed ring of different shapes such as circle, ellipse, square, rectangle, polygon.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101146612A CN100468042C (en) | 2006-11-21 | 2006-11-21 | Lucifugal and radiating structure for testing PCR fluorescence of micro flow control biologic chip |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2006101146612A CN100468042C (en) | 2006-11-21 | 2006-11-21 | Lucifugal and radiating structure for testing PCR fluorescence of micro flow control biologic chip |
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| CN1963461A true CN1963461A (en) | 2007-05-16 |
| CN100468042C CN100468042C (en) | 2009-03-11 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104641220A (en) * | 2012-09-18 | 2015-05-20 | 浦项工科大学校产学协力团 | Microfluidic chip having flow cell for absorbance detection and absorbance detection device including same |
| CN105784611A (en) * | 2016-03-04 | 2016-07-20 | 江苏大学 | Multi-unit integration experiment device and method based on micro-fluidic chip luminosity detection |
| CN112044374A (en) * | 2020-09-01 | 2020-12-08 | 杨欢 | Chip for chain polymerization based on microfluidic technology |
-
2006
- 2006-11-21 CN CNB2006101146612A patent/CN100468042C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104641220A (en) * | 2012-09-18 | 2015-05-20 | 浦项工科大学校产学协力团 | Microfluidic chip having flow cell for absorbance detection and absorbance detection device including same |
| CN104641220B (en) * | 2012-09-18 | 2017-05-24 | 浦项工科大学校产学协力团 | Microfluidic chip having flow cell for absorbance detection and absorbance detection device including same |
| CN105784611A (en) * | 2016-03-04 | 2016-07-20 | 江苏大学 | Multi-unit integration experiment device and method based on micro-fluidic chip luminosity detection |
| CN105784611B (en) * | 2016-03-04 | 2018-08-10 | 江苏大学 | Multiple-unit integration test apparatus and method based on micro-fluidic chip photometric detection |
| CN112044374A (en) * | 2020-09-01 | 2020-12-08 | 杨欢 | Chip for chain polymerization based on microfluidic technology |
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| CN100468042C (en) | 2009-03-11 |
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Granted publication date: 20090311 Termination date: 20091221 |