CN1963439A - Immunohistochemistry division bore dying paste, methods employed and purpose of the same - Google Patents
Immunohistochemistry division bore dying paste, methods employed and purpose of the same Download PDFInfo
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- CN1963439A CN1963439A CN 200610135250 CN200610135250A CN1963439A CN 1963439 A CN1963439 A CN 1963439A CN 200610135250 CN200610135250 CN 200610135250 CN 200610135250 A CN200610135250 A CN 200610135250A CN 1963439 A CN1963439 A CN 1963439A
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Abstract
This invention relates to immune group hole dye slice and its usage method, which comprises the following usage steps: sticking tightly more than one transparent holes film slices into load slice coated with organic coupon and dripping immune agent into each hole form immune group dying and the agent volume is limited to the hole edge; ripping off film slices or dipping the load slice into organic liquid to make film peeled off for sealing.
Description
Technical field
The present invention relates to a kind of SABC division bore dying paste and using method and purposes.
Background technology
When pair cell smear or histotomy carry out immunohistochemical staining on microslide, in order to control the dyeing scope, need earlier on cell smear or around the histotomy, to draw a circle in the past, in circle, carry out immunohistochemical staining then with the SABC pen of drawing a circle.But, can't adopt on than the cell smear of small size the draw a circle method of stroke circle of SABC to carry out multinomial immunohistochemical staining; On a histotomy, also can't adopt the draw a circle method of stroke circle of SABC to carry out multinomial immunohistochemical staining.Have only the very cell smear of small size and usually can run in the pathology work, or have only a histotomy, but need carry out the situation of multinomial immunohistochemical staining in the above.
Summary of the invention
In order to solve existing in prior technology the problems referred to above, the invention provides a kind of SABC division bore dying paste and using method and purposes, it not only can carry out multinomial immunohistochemical staining simultaneously on a cell smear or histotomy, and can save SABC reagent in a large number.
Technical scheme provided by the present invention is as follows:
(1) SABC division bore dying paste, it is characterized in that: comprise film or the thin slice of area less than microslide, be distributed with more than one penetrability aperture on film or the thin slice, leave certain distance between hole and the hole, the edge of hole and film or thin slice also leaves certain back gauge.
The adhesive sticker of the bottom surface by being applied to film or thin slice can closely be attached to this film or thin slice on the dry microslide that adheres to cell smear to be measured or histotomy.
For the ease of preserving the above-mentioned SABC division bore dying paste that makes, the bottom surface of this film or thin slice can be attached on the separate paper.During use film or thin slice are taken off from separate paper.
Distinguish different immunohistochemical staining projects for the ease of the operator, can around the hole of film or thin slice, be printed on the sequence number in hole.
Described film or thin slice preferably are made of plastics.
(2) adopt above-mentioned SABC division bore dying paste to carry out the using method of multinomial immunohistochemical staining, it is characterized in that: earlier above-mentioned film or the thin slice that is distributed with an above penetrability aperture taken off from separate paper, again this film or thin slice are closely sticked on the microslide that adheres to the dry cell smear or the histotomy that dewaxed, in each hole, drip SABC reagent respectively, carry out immunohistochemical staining, the amount of reagent of dropping is not to overflow degree of being from bore edges; After the dyeing film or thin slice are torn, maybe this microslide is immersed in the organic solvent, film or thin slice are come off, and then carry out mounting.
(3) purposes of above-mentioned SABC division bore dying paste: described SABC division bore dying paste is used in the multinomial immunohistochemical staining that carries out cell smear or histotomy on the microslide.
Than prior art, the present invention is owing to adopt the SABC division bore dying to be attached to the multinomial immunohistochemical staining that carries out cell smear or histotomy on the microslide, therefore has following remarkable advantage: because in advance film or thin slice are closely sticked on the microslide that adheres to the dry cell smear or the histotomy that dewaxed, just in hole spaced apart, drip SABC reagent afterwards and carry out immunohistochemical staining, therefore can effectively control the amount of reagent that each hole drips; (being limited in each hole) determined in the position that drips reagent, and is difficult for therefore can carrying out multinomial immunohistochemical staining simultaneously on a cell smear or histotomy to diffusion all around; Consequently improved work efficiency greatly, and the utilization factor of cell smear (or histotomy), SABC reagent also saved simultaneously in a large number.
Description of drawings
Fig. 1 is a kind of synoptic diagram of SABC division bore dying paste of the present invention.
Fig. 2 is the another kind of synoptic diagram of SABC division bore dying paste of the present invention.
Fig. 3 is that the SABC division bore dying paste with Fig. 1 is attached to the synoptic diagram on the separate paper.
Fig. 4 is the using method synoptic diagram of the SABC division bore dying paste of Fig. 1.
Specific implementation method
As Fig. 1 or Figure 2 shows that the SABC division bore dying paste of making, it comprises film or the thin slice 1 of area less than microslide; Be distributed with more than one penetrability aperture 2 on film or the thin slice, described hole is preferably circular port, leaves certain distance between hole and the hole, and the edge of hole and film or thin slice also leaves certain back gauge; Adhesive sticker on the coated on bottom side of above-mentioned film or thin slice; And around the hole of above-mentioned film or thin slice, be printed on the sequence number 4 in hole.Described film or thin slice preferably are made of plastics.
For the ease of preserving the above-mentioned SABC division bore dying paste of making, the bottom surface of above-mentioned film or thin slice is attached on the separate paper 3, as shown in Figure 3.
It is as follows to adopt above-mentioned SABC division bore dying paste to carry out the step of using method of multinomial immunohistochemical staining: the film or the thin slice that will be distributed with an above penetrability aperture are earlier taken off from separate paper, this film or thin slice are closely sticked on the microslide 5 that adheres to the dry cell smear or the histotomy that dewaxed again; Drip SABC reagent respectively in each hole, carry out immunohistochemical staining, the amount of reagent of dropping is not to overflow degree of being from bore edges; After the dyeing film or thin slice are torn, maybe this microslide is immersed in the organic solvent, film or thin slice are come off, and then carry out mounting.
Above-mentioned SABC division bore dying paste is used in the multinomial immunohistochemical staining that carries out cell smear or histotomy on the microslide.
Claims (6)
1. SABC division bore dying paste, it is characterized in that: comprise film or the thin slice (1) of area less than microslide, be distributed with more than one penetrability aperture (2) on film or the thin slice, leave certain distance between hole and the hole, the edge of hole and film or thin slice also leaves certain back gauge.
2. SABC division bore dying paste according to claim 1 is characterized in that: the bottom surface of described film or thin slice scribbles adhesive sticker, and the bottom surface of this film or thin slice is attached on the separate paper (3).
3. SABC division bore dying paste according to claim 1 is characterized in that: the sequence number (4) that is printed on the hole around the hole of described film or thin slice.
4. SABC division bore dying paste according to claim 1 is characterized in that: described film or thin slice (1) are made of plastics.
5. adopt the using method of carrying out multinomial immunohistochemical staining as each described SABC division bore dying paste in the claim 1～4, it is characterized in that: the film or the thin slice that will be distributed with an above penetrability aperture closely stick on the microslide (5) of attached cell smear or histotomy (6), in each hole, drip SABC reagent respectively, carry out immunohistochemical staining, the amount of reagent of dropping is not to overflow degree of being from bore edges; After the dyeing film or thin slice are torn, maybe this microslide is immersed in the organic solvent, film or thin slice are come off, and then carry out mounting.
6. as the purposes of each described SABC division bore dying paste in the claim 1～4, it is characterized in that: described SABC division bore dying paste is used in the multinomial immunohistochemical staining that carries out cell smear or histotomy on the microslide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610135250 CN1963439A (en) | 2006-11-27 | 2006-11-27 | Immunohistochemistry division bore dying paste, methods employed and purpose of the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610135250 CN1963439A (en) | 2006-11-27 | 2006-11-27 | Immunohistochemistry division bore dying paste, methods employed and purpose of the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1963439A true CN1963439A (en) | 2007-05-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610135250 Pending CN1963439A (en) | 2006-11-27 | 2006-11-27 | Immunohistochemistry division bore dying paste, methods employed and purpose of the same |
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| CN (1) | CN1963439A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102183400A (en) * | 2011-01-31 | 2011-09-14 | 浙江世纪康大医疗科技有限公司 | Dyeing instrument antibody accommodating device |
| CN105973681A (en) * | 2016-05-19 | 2016-09-28 | 四川金域医学检验中心有限公司 | Immunohistochemical operation method |
| CN108037278A (en) * | 2017-12-13 | 2018-05-15 | 广州吉奥生物科技有限责任公司 | The preparation method of immunohistochemistry detection section |
| CN110441118A (en) * | 2019-08-28 | 2019-11-12 | 广东医科大学 | A kind of box-packed immunohistochemistry pen and its preparation method and application method |
-
2006
- 2006-11-27 CN CN 200610135250 patent/CN1963439A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102183400A (en) * | 2011-01-31 | 2011-09-14 | 浙江世纪康大医疗科技有限公司 | Dyeing instrument antibody accommodating device |
| CN102183400B (en) * | 2011-01-31 | 2012-11-14 | 浙江世纪康大医疗科技有限公司 | Dyeing instrument antibody accommodating device |
| CN105973681A (en) * | 2016-05-19 | 2016-09-28 | 四川金域医学检验中心有限公司 | Immunohistochemical operation method |
| CN105973681B (en) * | 2016-05-19 | 2018-12-28 | 四川金域医学检验中心有限公司 | A kind of immunohistochemistry operating method |
| CN108037278A (en) * | 2017-12-13 | 2018-05-15 | 广州吉奥生物科技有限责任公司 | The preparation method of immunohistochemistry detection section |
| CN108037278B (en) * | 2017-12-13 | 2018-09-25 | 广州吉奥生物科技有限责任公司 | The preparation method of immunohistochemistry detection slice |
| CN110441118A (en) * | 2019-08-28 | 2019-11-12 | 广东医科大学 | A kind of box-packed immunohistochemistry pen and its preparation method and application method |
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Open date: 20070516 |