CN1962848A - Red monascus strain Danxi-1 - Google Patents
Red monascus strain Danxi-1 Download PDFInfo
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- CN1962848A CN1962848A CN 200510061479 CN200510061479A CN1962848A CN 1962848 A CN1962848 A CN 1962848A CN 200510061479 CN200510061479 CN 200510061479 CN 200510061479 A CN200510061479 A CN 200510061479A CN 1962848 A CN1962848 A CN 1962848A
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Abstract
The invention relates to a microorganism, in particular to a monascus strain capable of obtaining high content of gamma-reanal and Lovastatin, the strain is named Monascus ruber Danxi-1, which is preserved in SIPO designated China General Microbiological Culture Collection Center with a docket number of CGMCC No: 1405, the preservation date being June 10, 2005.
Description
Technical field
The present invention relates to a kind of microorganism, especially relate to a kind of monascus strain that can obtain high gamma-aminobutyric acid and high lovastatin content.
Background technology
γ-An Jidingsuan (γ-Aminobuty γ ic acid, be called for short GABA), be found to be the inhibitory nerve transmitter (Inhibito γ y neu γ ot γ ansmitte γ s) of vertebrates sympathetic nervous system cell binding site very early, because GABA has regulating effect to sympathetic nervous system, therefore play an important role in the cardiovascular function of vertebrates.γ-An Jidingsuan also is a kind of natural nonprotein amino acid, be prevalent in the nature animals and plants, in human body, far from nineteen fifty medical circle just find that GABA is present in the cerebellar cortex and spinal cord and retina of human body, and the GABA tool has efficacy in lowering high blood pressure and also far just is found from the latter stage fifties.GABA why can be hypotensive mechanism can to block peripheral nerve conduction active relevant with GABA by inference, generation nineteen sixty, Stanton finds that GABA reaches 20% o'clock desired concn to various animals is hypotensive, the animal of per kilogram of body weight, dog needs 11 micrograms, rabbit 19 micrograms, pig 174 micrograms, the hypertension mouse that is caused for the feeding high salt diet, with the average about 4 milligrams of GABA of picked-up every day, the result shows that hypertension is after 4 weeks, blood pressure just can fall and reach the above unusual effect of 30mmHg, in addition, also have the medical research report of a lot of pure GABA aminobutyric acids to the nerve conduction system from the latter stage seventies to the eighties, these research reports also all confirm the effect that GABA can be hypotensive.
The known pure natural food that contains GABA mainly contains Japanese Jia Yelong tea, red colouring agent for food, also used as a Chinese medicine, Sprout rice, fermented soybean, mulberry leaf, soft-shelled turtle etc. at present.But GABA content is lower in these common materials, can not meet the demand of people to health.
Research is in recent years found again can become to produce a kind of very strong lipopenicillinase active substance one lovastatin in the red koji fermentation process, but the lovastatin content that the red colouring agent for food, also used as a Chinese medicine bacterial strain that adopts in the existing zymotechnique produces is too low.
Summary of the invention
It is lower to the present invention is directed to whole food GABA content of the prior art, and the lovastatin content that the red colouring agent for food, also used as a Chinese medicine bacterial strain that adopts in the existing zymotechnique produces is too low, can not meet the shortcoming of people to the demand of health; Providing a kind of is matrix with the rice, from tunning, obtains fungi microbe---the red monascus strain Danxi-1 of high GABA content, high lovastatin content.
Red monascus strain Danxi-1 called after Monascus ruber red monascus Danxi-1 of the present invention, screen in the substratum of Monas cuspurpureus Went distiller grains enlarged culturing, this bacterial strain is in the preservation of specified depositary institution of State Intellectual Property Office, preservation date is on June 10th, 2005, depositary institution's title: China Committee for Culture Collection of Microorganisms common micro-organisms center, deposit number: CGMCC NO:1405.
The cultural characteristic of red monascus strain Danxi-1 of the present invention is as follows:
Cultivated 25 days for 30 ℃ on the wort agar, the bacterium colony quality is tightr, and felted is smooth, and there is a small amount of radial radiating ridge at the back side, the expansion growth, and the edge is smooth, mycelium initial stage velveteen shape, fallen or falling flowers pink colour, aging back rose pink colour;
Cultivated 25 days for 30 ℃ on the MEA substratum, colony growth is smooth, and broken edge is petal-shaped, has a small amount of aerial hyphae to form, and the initial stage gold is entangled yellow, and the light cocoa in aging back is brown;
Cultivated 25 days for 30 ℃ on the CYA substratum, colony growth is thin, white, and the back side does not have radial fold.
Above culture does not all have secretory product and pigment to generate.
The morphological specificity of the bacterial strain of red monascus strain Danxi-1 of the present invention:
The irregular branch of mycelium, have every, produce orange-yellow oil droplet, the about 3-6 μ of hyphal diameter m, conidium exists, many Dan Sheng, ocean pyriform or sphere, 14 * 8-9 μ m, quilt device light orange or colourless, subsphaeroidal 25-37 μ m, thecaspore in the thicker about 2-3 μ m of wall, most quilt device forms light orange or colourless, subsphaeroidal 25-37 μ m, thecaspore in the thicker about 2-3 μ m of wall, most quilt device forms not good enough; Thecaspore is oval or subsphaeroidal, smooth, faint yellow, 5.0-5.8 * 4.3-5.2 μ m, 4.6-5.4 μ m.
On wort agar substratum and potato nutrient agar, under 30 ℃ of 10 days conditions, for cultured continuously, its cultural characteristic and morphological specificity have no significant change red monascus strain Danxi-1 of the present invention through number respectively, and the biological character of this bacterium is basicly stable.
Red monascus strain Danxi-1 of the present invention on June 10th, 2005 in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, deposit number is: CGMCCNO:1405, classification called after: Monascus ruber red monascus Danxi-1, depositary institution address: China. Beijing. Institute of Microorganism, Academia Sinica, postcode: 100080
The microorganism that the present invention relates to, this microorganism can be with rice as fermented substrate, by artificial pure culture mode, and the functional red yeast rice that obtains, compare with original common red koji fermentation product, variation has all taken place in the content of γ-An Jidingsuan and lovastatin.
A. the Monas cuspurpureus Went vinegar of brewageing with traditional method detects through HPLC, and it is 36mg/L. that its spit of fland content is cut down in total Lip river
B. by the above-mentioned red rice vinegar with fat-reducing function of improving one's methods and making, detect through HPLC, it is 300mg/L. that its spit of fland content is cut down in total Lip river
C. red monascus strain involved in the present invention can produce a large amount of L-Glutamic decarboxylases and can utilize the L-glutamic acid in the rice to make it generate a large amount of γ-An Jidingsuans, and detecting alpha-aminobutyric acid content through HPLC is 720mg/kg.
Description of drawings
Fig. 1 is the bacterium colony figure of red monascus strain Danxi-1 of the present invention on the wort agar substratum
Fig. 2 is the bacterium colony figure of red monascus strain Danxi-1 of the present invention on the MEA substratum
Fig. 3 is the bacterium colony figure of red monascus strain Danxi-1 of the present invention on the CYA substratum
Embodiment
Below be specific embodiments of the invention and in conjunction with the accompanying drawings, technical scheme of the present invention is further described, but the present invention be not limited to these embodiment
Embodiment
Get Danxi-1 (red monascus strain Danxi-1) bacterial classification, under aseptic condition (sterilisable chamber or Bechtop), with a little bacterial classification of inoculation picking, transfer in sterilized solid MEA culture medium culturing ware, placed interior 30 ℃ ± 1 ℃ activation culture of incubator 120 hours.Take out 120 hours bacterial classification of activation, under aseptic condition, insert the good bacterial classification of activation in one-level solid MEA substratum, cultivated 120 hours, be the Danxi-1 first order seed at 30 ℃ ± 1 ℃ with sterilization with the same manner.
Adopt 500 milliliters of glass triangle bottles, 100 milliliters of the liquid MEA substratum that prepare of packing into, 15 pounds of sterilizations 30 minutes are standby.Get the Danxi-1 first order seed, under aseptic condition, be inoculated in and be equipped with in 500 milliliters of glass triangle bottles of 100 milliliters of liquid MEA substratum, the first order seed inoculum size connects five triangular flasks for each culture dish, after cultivating 120 hours to 30 ℃ ± 1 ℃ shaking table, use as the red koji fermentation secondary seed.
According to the fermentation scale, ratio is by weight expected in fermentation, takes by weighing rice, fully stirs, and fermentation material moisture is not less than 65%.Plastics film, kraft paper seal, and put in the Autoclave 15 pounds of sterilizations 60 minutes, takes out to be cooled to 30 ℃, access liquid secondary seed, the access amount of secondary seed is 5% (V/W).Under placing 30 ℃ ± 1 ℃ after shaking up, standing for fermentation 25 days.During fermentation, notice that proving room is preserved moisture to be not less than 80% and to read relatively, shook bottle respectively once on the 5th day, and note checking and have or not the microbiological contamination phenomenon.
Fermentation finishes, and tunning to 35～50 ℃ oven dry down gets functional red yeast rice.
The present invention is that red monascus strain Danxi-1 (Monascus ruber) gets rice fermentation, adopt artificial solid fermentation method, tunning and common red koji fermentation product compare: the Monas cuspurpureus Went vinegar with traditional method is brewageed, to detect through HPLC, and it is 36mg/L that its spit of fland content is cut down in total Lip river; By the above-mentioned red rice vinegar with fat-reducing function of improving one's methods and making, to detect through HPLC, it is 300mg/L that its spit of fland content is cut down in total Lip river; Red monascus strain involved in the present invention can produce a large amount of L-Glutamic decarboxylases and can utilize the L-glutamic acid in the rice to make it generate a large amount of γ-An Jidingsuans, and detecting alpha-aminobutyric acid content through HPLC is 720mg/kg.
In sum, through red monascus strain Danxi-1 (Monascus ruber) ferment rice can produce content higher the Lip river cut down its spit of fland and γ-An Jidingsuan; The another natural microbial medicine source of screening treatment hyperlipidemia and hypertension medicinal ingredients.
Specific embodiment described in the present invention only is that the present invention's spirit is illustrated.The technician of the technical field of the invention can make various modifications or replenishes or adopt similar mode to substitute described specific embodiment, but can't depart from spirit of the present invention or surmount the defined scope of appended claims.
Although the present invention has been made detailed explanation and has quoted some specific exampless as proof, to those skilled in the art, only otherwise leave that the spirit and scope of the present invention can be done various variations or correction is obvious.
Claims (4)
1, a kind of red monascus strain Danxi-1, it is characterized in that called after Monascus ruber red monascus Danxi-1, this bacterial strain is in the preservation of specified depositary institution of State Intellectual Property Office, preservation date is on June 10th, 2005, depositary institution's title: China Committee for Culture Collection of Microorganisms common micro-organisms center, deposit number: CGMCC NO:1405.
2, red monascus strain Danxi-1 according to claim 1 is characterized in that the cultural characteristic of described red monascus strain Danxi-1 is as follows:
Cultivated 25 days for 30 ℃ on the wort agar, the bacterium colony quality is tightr, and felted is smooth, and there is a small amount of radial radiating ridge at the back side, the expansion growth, and the edge is smooth, mycelium initial stage velveteen shape, fallen or falling flowers pink colour, aging back rose pink colour;
Cultivated 25 days for 30 ℃ on the MEA substratum, colony growth is smooth, and broken edge is petal-shaped, has a small amount of aerial hyphae to form, and the initial stage gold is entangled yellow, and the light cocoa in aging back is brown;
Cultivated 25 days for 30 ℃ on the CYA substratum, colony growth is thin, white, and the back side does not have radial fold.
3, red monascus strain Danxi-1 according to claim 1 is characterized in that the morphological specificity of the bacterial strain of described red monascus strain Danxi-1:
The irregular branch of mycelium, have every, produce orange-yellow oil droplet, the about 3-6 μ of hyphal diameter m, conidium exists, many Dan Sheng, ocean pyriform or sphere, 14 * 8-9 μ m, quilt device light orange or colourless, subsphaeroidal 25-37 μ m, thecaspore in the thicker about 2-3 μ m of wall, most quilt device forms light orange or colourless, subsphaeroidal 25-37 μ m, thecaspore in the thicker about 2-3 μ m of wall, most quilt device forms not good enough; Thecaspore is oval or subsphaeroidal, smooth, faint yellow, 5.0-5.8 * 4.3-5.2 μ m, 4.6-5.4 μ m.
4, red monascus strain Danxi-1 according to claim 1 is characterized in that the The stability of strain feature of described red monascus strain Danxi-1:
On wort agar substratum and potato nutrient agar, under 30 ℃ of 10 days conditions, for cultured continuously, its cultural characteristic and morphological specificity have no significant change through number respectively, and the biological character of this bacterium is basicly stable.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101760477B (en) * | 2009-08-11 | 2011-11-16 | 杭州千岛湖星遥实业有限公司 | Manufacturing method of blood pressure lowering red rice |
| CN101333483B (en) * | 2007-06-25 | 2011-12-07 | 义乌市丹溪酒业有限公司 | Red rice yellow wine with antihypertensive function |
| CN103409444A (en) * | 2013-07-10 | 2013-11-27 | 浙江师范大学 | CDNA (Complementary Deoxyribose Nucleic Acid) nucleotide of monascus ruber GAD (Glutamic Acid Decarboxylase) gene and synthetic method thereof and corresponding protein |
| CN104263652A (en) * | 2014-08-11 | 2015-01-07 | 北京东方红航天生物技术股份有限公司 | Monascus purpureus mutant strain for producing lovastatin and mutagenesis and screening method of monascus purpureus mutant strain |
| CN108977473A (en) * | 2018-01-29 | 2018-12-11 | 北大方正集团有限公司 | A kind of preparation method of fermenting and producing Lovastatin rice Spore cultivation base and rice spore |
| CN109234348A (en) * | 2018-06-27 | 2019-01-18 | 天水师范学院 | A kind of antidepression reducing blood lipid Hongqu powder (red colouring agent) and preparation method thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101880691B (en) * | 2010-01-14 | 2012-11-07 | 陈豪锋 | Preparation method for brewing function red yeast rice with low-yield citrinin |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BG61613B1 (en) * | 1994-06-24 | 1998-01-30 | Анна Куюмджиева | Strain monascus purpureus 94-25, producent of pigment andaccompanying products |
| CN1059463C (en) * | 1997-01-13 | 2000-12-13 | 丁前胜 | Violet monascus, monascus and their application. |
| CN1059464C (en) * | 1997-01-13 | 2000-12-13 | 丁前胜 | Monascus with new strain and its application |
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2005
- 2005-11-09 CN CNB2005100614790A patent/CN100443583C/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101333483B (en) * | 2007-06-25 | 2011-12-07 | 义乌市丹溪酒业有限公司 | Red rice yellow wine with antihypertensive function |
| CN101760477B (en) * | 2009-08-11 | 2011-11-16 | 杭州千岛湖星遥实业有限公司 | Manufacturing method of blood pressure lowering red rice |
| CN103409444A (en) * | 2013-07-10 | 2013-11-27 | 浙江师范大学 | CDNA (Complementary Deoxyribose Nucleic Acid) nucleotide of monascus ruber GAD (Glutamic Acid Decarboxylase) gene and synthetic method thereof and corresponding protein |
| CN104263652A (en) * | 2014-08-11 | 2015-01-07 | 北京东方红航天生物技术股份有限公司 | Monascus purpureus mutant strain for producing lovastatin and mutagenesis and screening method of monascus purpureus mutant strain |
| CN104263652B (en) * | 2014-08-11 | 2017-01-11 | 北京东方红航天生物技术股份有限公司 | Monascus purpureus mutant strain for producing lovastatin and mutagenesis and screening method of monascus purpureus mutant strain |
| CN108977473A (en) * | 2018-01-29 | 2018-12-11 | 北大方正集团有限公司 | A kind of preparation method of fermenting and producing Lovastatin rice Spore cultivation base and rice spore |
| CN109234348A (en) * | 2018-06-27 | 2019-01-18 | 天水师范学院 | A kind of antidepression reducing blood lipid Hongqu powder (red colouring agent) and preparation method thereof |
| CN109234348B (en) * | 2018-06-27 | 2022-01-07 | 天水师范学院 | Anti-depression blood fat reducing monascus powder and preparation method thereof |
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| CN100443583C (en) | 2008-12-17 |
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Effective date of registration: 20161101 Address after: 322003, No. 117, Dan Xi Road, red town, Yiwu, Zhejiang Patentee after: Yiwu Danxi Wine Industry Co., Ltd. Address before: 322003, No. 117, Dan Xi Road, red town, Yiwu, Zhejiang Patentee before: Zhu Lanqin |