CN1952172A - 检测十字花科植物在胁迫条件下基因表达信息的cDNA芯片 - Google Patents
检测十字花科植物在胁迫条件下基因表达信息的cDNA芯片 Download PDFInfo
- Publication number
- CN1952172A CN1952172A CN 200510109294 CN200510109294A CN1952172A CN 1952172 A CN1952172 A CN 1952172A CN 200510109294 CN200510109294 CN 200510109294 CN 200510109294 A CN200510109294 A CN 200510109294A CN 1952172 A CN1952172 A CN 1952172A
- Authority
- CN
- China
- Prior art keywords
- dna
- artificial sequence
- probe
- gene
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
本发明公开了一种检测十字花科植物生物及非生物胁迫下基因表达信息的cDNA芯片。该基因芯片以逆境下和特殊发育时期大白菜基因的cDNA片段作为探针,由序列表中SEQ ID №:1-221DNA序列的探针,以及3885条GenBank中的探针序列组成。本发明不仅可用于批量检测十字花科植物在生物或非生物胁迫下的基因表达信息、筛选差异基因,还可用于构建十字花科植物不同生长发育阶段、不同组织器官乃至特异细胞中的基因表达谱。应用该芯片可实现基因表达信息的高通量检测,且操作方便、安全,检测结果可靠、有效,可节省大量时间、人力、物力和财力,具有广阔的应用前景。
Description
技术领域
本发明涉及一种检测植物在胁迫条件下基因表达信息的cDNA芯片,特别是涉及一种检测十字花科植物在病、虫等生物及干旱、寒冷等非生物胁迫下基因表达信息的cDNA芯片。
背景技术
利用已知基因信息在待测样品中特异地检测目的基因的表达信息,是分子生物学领域中常用的检测手段,如常用的Southern杂交、Northern杂交以及PCR技术。但是,随着分子生物学的发展,这些技术不能在同一条件下对成千上万个基因同时进行分析,因此具有一定的局限性。通过原位合成(in situ synthesis)探针或合成目的基因片段后采用交联的方式,可以将成千上万个基因固定在载体上,再利用杂交方式检测基因表达信息的方法已成为一种趋势。在应用领域,生物芯片可广泛地用于医学临床诊断、药物开发、环境监测、食品卫生监督以及司法鉴定等;在科研领域,生物芯片主要用于检测生物体在不同发育阶段、组织器官、及在各种生物及非生物胁迫下基因的表达情况,鉴定特异表达基因的信息,从而为研究提供信息和方向。
通过构建cDNA文库、测序获取基因信息,是生物芯片制备过程中目的探针设计的主要来源。目前,在一些大型数据库(如NCBI上的GenBank)中已经有多种生物基因的EST(基因表达序列标签)、基因、甚至基因组序列信息,为一些模式生物芯片的制作提供了信息,大大方便了生物基因表达信息的获取。探针制作完成后,通过将液相化学合成的大量探针,或将PCR扩增的cDNA、基因组DNA经纯化、定量分析后,通过阵列点样机(arrayer)及电脑控制的机器手,准确、快速地将不同探针样品定量点样于带正电荷的尼龙膜或多聚赖氨酸包被的硅片等载体的相应位置上,再由紫外线交联固定后即得到DNA微阵列或芯片。而常规的杂交检测方法主要包括目的片段扩增、直接点样或电泳后转到尼龙膜上、紫外铰链等步骤,因此,整体需要目的片段的量较大,且每次只能分析少数基因,具有一定的局限性。
待测的生物样品往往是各种组分的混合体,成分非常复杂,直接用样品本身进行标记杂交对样品量的要求较高。因此,待测样品DNA在杂交前一般都要通过聚合酶链反应(PCR)进行扩增,然后对扩增后的靶DNA进行标记。芯片杂交可以根据实际情况在常规杂交技术的基础上进行优化,然后进行扫描,图像处理、数据提取、进行统计分析,最终得到结果。这种处理和分析数据方式比常规杂交检测的灵敏度高,尤其是对一些低丰度表达的基因,此外,通过统计学进行分析可提高结果的准确性。
发明内容
本发明的目的是一种检测十字花科植物在病、虫等生物及干旱、寒冷等非生物胁迫下基因表达信息的cDNA芯片。
本发明所提供的检测十字花科植物在胁迫条件下基因表达信息的cDNA芯片,探针序列来源主要包括:序列表中SEQ ID №:1-221 DNA序列以及具有下述GenBank登录号的探针序列:
CA991436,CA991442,CA991443,CA991447-CA991450,CA991452-CA991458,CA991460,CA991462,CA991463,CA991465-CA991470,CA991473-CA991482,CA991484-CA991493,CA991495-CA991500,CA991502,CA991503,CA991505,CA991508,CA991510-CA991522,CA991524,CA991525,CA991527,CA991528,CA991530,CA991532,CA991534,CA991536-CA991540,CA991542,CA991544-CA991546,CA991548,CA991549,CA991551-CA991566,CA991568-CA991572,CA991574-CA991577,CA991579,CA991581,CA991582,CA991585-CA991603,CA991606-CA991610,CA991616-CA991654,CA991666-CA991668,CA991671,CA991673-CA991675,CA991678-CA991681,CA991686-CA991693,CA991698-CA991700,CA991705-CA991707,CA991710,CA991714,CA991715,CA991718-CA991720,CA991723,CA991725-CA991727,CA991729-CA991733,CA991735,CA991737-CA991763,CA991770-CA991773,CA991776-CA991778,CA991781,CA991784-CA991790,CA991792,CA991793,CA991795,CA991797-CA991804,CA991806-CA991808,CA991814,CA991817-CA991822,CA991826-CA991832,CA991834,CA991835,CA991839-CA991842,CA991848-CA991852,CA991854,CA991855,CA991857,CA991858-CA991860,CA991866,CA991869,CA991872-CA991875,CA991877,CA991879-CA991882,CA991887,CA991892,CA991895-CA991898,CA991900,CA991901,CA991903-CA991905,CA991907,CA991909,CA991911,CA991912,CA991915-CA991927,CA991930,CA991934,CA991936-CA991944,CA991946-CA991954,CA991960,CA991961,CA991964,CA991966,CA991968-CA991973,CA991976-CA991984,CA991987,CA991989,CA991991-CA991995,CA992000-CA992002,CA992005,CA992007,CA992008,CA992010-CA992012,CA992015,CA992017-CA992025,CA992028,CA992032,CA992033,CA992037,CA992041-CA992045,CA992053-CA992057,CA992061,CA992062,CA992065-CA992069,CA992072-CA992076,CA992080,CA992083,CA992086,CA992088-CA992091,CA992093-CA992097,CA992101,CA992105,CA992114,CA992118,CA992120-CA992122,CA992125,CA992127,CA992130,CA992132,CA992138-CA992146,CA992148,CA992150,CA992155,CA992159,CA992161,CA992163,CA992169-CA992172,CA992175-CA992178,CA992182-CA992192,CA992195,CA992198,CA992202,CA992203,CA992206-CA992208,CA992212,CA992214,CA992215,CA992218,CA992219,CA992221,CA992223-CA992225,CA992227,CA992229,CA992232-CA992234,CA992237,CA992241,CA992247-CA992249,CA992253,CA992254,CA992259-CA992267,CA992272,CA992273,CA992277-CA992283,CA992287-CA992289,CA992292-CA992296,CA992299,CA992303-CA992305,CA992308,CA992310,CA992315,CA992319,CA992324-CA992333,CA992336,CA992338-CA992350,CA992355,CA992356,CA992359-CA992366,CA992373,CA992376,CA992378,CA992380,CA992385-CA992388,CA992391,CA992392,CA992394,CA992396,CA992400,CA992401,CA992406-CA992418,CA992421-CA992424,CA992428,CA992431,CA992432,CA992434,CA992436,CA992439-CA992442,CA992445-CA992448,CA992450,CA992451,CA992458-CA992461,CA992464-CA992473,CA992476-CA992481,CA992484,CA992487,CA992488,CA992492,CA992496,CA992498-CA992504,CA992507,CA992508,CA992510-CA992513,CA992516-CA992524,CA992530-CA992538,CA992540,CA992542-CA992546,CA992553-CA992569,CA992573-CA992579,CA992583-CA992585,DN191370,DN191373-DN191379,DN191385,DN191388-DN191391,DN191394-DN191397,DN191399-DN191401,DN191403,DN191406-DN191413,DN191417,DN191419,DN191422,DN191424,DN191429,DN191430,DN191433-DN191435,DN191438-DN191443,DN191445,DN191450,DN191453,DN191456,DN191458-DN191462,DN191464,DN191467,DN191468,DN191471,DN191473,DN191474,DN191476,DN191479,DN191483,DN191485-DN191488,DN191491,DN191492,DN191497,DN191498,DN191502,DN191503,DN191506,DN191507,DN191510-DN191526,DN191530-DN191532,DN191534-DN191536,DN191538,DN191543,DN191544,DN191546,DN191548-DN191554,DN191556,DN191559-DN191561,DN191563,DN191564,DN191570,DN191572,DN191573,DN191575-DN191577,DN191579,DN191582-DN191586,DN191588-DN191590,DN191594,DN191597,DN191599,DN191603-DN191607,DN191609-DN191618,DN191620-DN191626,DN191629,DN191631-DN191634,DN191636-DN191638,DN191642-DN191644,DN191647,DN191649,DN191651,DN191653-DN191655,DN191659,DN191662,DN191665,DN191666,DN191670,DN191671,DN191673-DN191677,DN191679-DN191682,DN191685-DN191689,DN191693-DN191696,DN191698,DN191702,DN191703,DN191705-DN191712,DN191714-DN191722,DN191724,DN191726,DN191728-DN191730,DN191733,DN191737-DN191739,DN191741,DN191743-DN191745,DN191747-DN191749,DN191751,DN191753,DN191754,DN191757-DN191760,DN191763,DN191765,DN191766,DN191769,DN191771-DN191773,DN191775,DN191777-DN191782,DN191784,DN191787-DN191791,DN191793-DN191795,DN191797-DN191806,DN191808,DN191809,DN191811,DN191813,DN191814,DN191816-DN191822,DN191826,DN191828,DN191832-DN191846,DN191848,DN191850-DN191853,DN191856-DN191863,DN191866,DN191868,DN191869,DN191871,DN191872,DN191875,DN191877-DN191890,DN191891-DN191895,DN191898-DN191900,DN191903-DN191910,DN191914-DN191919,DN191921-DN191926,DN191930-DN191933,DN191937-DN191948,DN191950,DN191953,DN191955-DN191960,DN191962,DN191967,DN191970-DN191972,DN191979,DN191984,DN191986-DN191989,DN191992-DN191994,DN191997,DN192001-DN192005,DN192010,DN192012,DN192017,DN192019-DN192024,DN192026,DN192027,DN192036,DN192037,DN192039-DN192042,DN192044,DN192046,DN192047,DN192049,DN192051,DN192052,DN192054-DN192062,DN192064-DN192067,DN192069--DN192074,DN192076-DN192081,DN192084,DN192085,DN192087,DN192088,DN192090-DN192092,DN192094,DN192095,DN192098-DN192100,DN192103-DN192107,DN192110,DN192112,DN192116,DN192118,DN192120-DN192123,DN192126,DN192128,DN192129,DN192131,DN192133-DN192135,DN192137,DN192138,DN192141,DN192142,DN192145,DN192146,DN192148,DN192150,DN192151,DN192153,DN192157,DN192160,DN192163,DN192164,DN192169,DN192170,DN192172,DN192174,DN192176,DN192179,DN192182-DN192184,DN192186,DN192188-DN192190,DN192192,DN192193,DN192195,DN192199,DN192201-DN192205,DN192207,DN192208,DN192209,DN192211,DN192214,DN192216,DN192219,DN192220,DN192223,DN192225,DN192226,DN192228,DN192230-DN192233,DN192236-DN192238,DN192240,DN192243-DN192247,DN192251-DN192255,DN192257,DN192258,DN192260-DN192263,DN192265-DN192269,DN192271,DN192273,DN192274,DN192276-DN192278,DN192280,DN192281,DN192289,DN192290,DN192293-DN192295,DN192298-DN192300,DN192302-DN192311,DN192314-DN192317,DN192319-DN192321,DN192323,DN192325,DN192327-DN192331,DN192334,DN192336-DN192345,DN192347,DN192348,DN192353,DN192354,DN192357,DN192359,DN192360,DN192362-DN192365,DN192367,DN192370,DN192374,DN192375,DN192377,DN192379,DN192381,DN192382,DN192384,DN192385,DN192389-DN192391,DN192393,DN192398,DN192402,DN192404,DN192406-DN192409,DN192414-DN192416,DN192418,DN192419,DN192421,DN192422,DN192424-DN192426,DN192429-DN192432,DN192434,DN192438-DN192440,DN192447-DN192450,DN192452-DN192454,DN192456,DN192458-DN192461,DN192464,DN192465,DN192467-DN192473,DN192475,DN192479,DN192483,DN192486-DN192489,DN192491,DN192493,DN192495,DN192497,DN192499,DN192501,DN192503-DN192505,DN192507-DN192511,DN192513-DN192515,DN192517,DN192519,DN192521,DN192524-DN192527,DN192529,DN192530,DN192532,DN192536-DN192544,DN192546,DN192547,DN192549,DN192552,DN192554-DN192559,DN192561-DN192563,DN192565,DN192566,DN192568-DN192571,DN192574,DN192576,DN960311-DN960316,DN960318,DN960327,DN960329,DN960332,DN960333,DN960335,DN960341,DN960344,DN960350,DN960353-DN960356,DN960359,DN960373,DN960379-DN960382,DN960384,DN960385,DN960388,DN960389,DN960391,DN960393,DN960394,DN960402-DN960404,DN960407,DN960410,DN960412,DN960419,DN960422,DN960424,DN960426,DN960427,DN960429-DN960431,DN960436,DN960438-DN960440,DN960442,DN960443,DN960446,DN960450,DN960453,DN960458,DN960460-DN960462,DN960465,DN960466,DN960468,DN960471,DN960473,DN960476,DN960478,DN960482,DN960483,DN960485,DN960487,DN960489,DN960491,DN960493,DN960494,DN960496,DN960498-DN960501,DN960505,DN960506,DN960510,DN960511,DN960513-DN960518,DN960521-DN960524,DN960527-DN960529,DN960532,DN960535-DN960538,DN960540,DN960541,DN960542,DN960544,DN960546,DN960547,DN960549,DN960553,DN960555,DN960556,DN960557,DN960559,DN960562,DN960563,DN960565,DN960568,DN960569,DN960571,DN960572,DN960575,DN960577,DN960580,DN960582,DN960584,DN960587,DN960589,DN960592,DN960594,DN960595,DN960597,DN960599,DN960601,DN960603,DN960604,DN960606,DN960611,DN960621,DN960628,DN960633,DN960636,DN960639,DN960643,DN960651,DN960654,DN960657,DN960661,DN960665,DN960666,DN960668,DN960670-DN960673,DN960675,DN960676,DN960685,DN960691,DN960693,DN960694,DN960695,DN960698,DN960700,DN960701,DN960713,DN960716-DN960718,DN960720,DN960727,DN960728,DN960734,DN960736-DN960740,DN960747,DN960750-DN960752,DN960757,DN960759,DN960764,DN960766-DN960770,DN960773,DN960774,DN960776-DN960779,DN960783-DN960785,DN960793-DN960801,DN960803,DN960809,DN960811-DN960813,DN960815-DN960817,DN960821,DN960825,DN960829,DN960832,DN960833,DN960835,DN960837-DN960841,DN960843,DN960844,DN960846-DN960855,DN960858,DN960862,DN960864,DN960868,DN960870-DN960874,DN960876,DN960878,DN960882,DN960883,DN960887,DN960894,DN960896-DN960899,DN960901,DN960903,DN960904,DN960909,DN960915-DN960917,DN960925,DN960930-DN960932,DN960934,DN960939,DN960940,DN960942,DN960944,DN960948,DN960952,DN960956,DN960959,DN960961,DN960964,DN960969,DN960970,DN960974,DN960977,DN960979-DN960982,DN960984,DN960986,DN960987,DN960991,DN960994,DN960995,DN960997,DN961007,DN961017-DN961020,DN961022,DN961024,DN961027,DN961032,DN961034,DN961035,DN961039,DN961042,DN961043,DN961045,DN961047,DN961048,DN961051,DN961053-DN961055,DN961057-DN961060,DN961064,DN961069,DN961072,DN961074,DN961077,DN961079,DN961080,DN961084,DN961086,DN961091,DN961093,DN961097,DN961101,DN961105,DN961107,DN961108,DN961110-DN961112,DN961114,DN961116,DN961120,DN961122-DN961126,DN961129,DN961132-DN961146,DN961148,DN961151,DN961152,DN961154,DN961156,DN961161,DN961164-DN961166,DN961169,DN961170,DN961172-DN961177,DN961180-DN961183,DN961185,DN961186,DN961188,DN961190,DN961194,DN961200,DN961201,DN961203,DN961204,DN961208,DN961211-DN961213,DN961215,DN961217,DN961219-DN961223,DN961225-DN961227,DN961229,DN961231,DN961234,DN961238,DN961240,DN961242,DN961245,DN961247,DN961249,DN961253-DN961255,DN961261,DN961262,DN961265,DN961268,DN961269,DN961271-DN961274,DN961278,DN961279,DN961283-DN961285,DN961293,DN961295-DN961297,DN961300,DN961303,DN961305-DN961308,DN961311,DN961316,DN961318,DN961320-DN961322,DN961324-DN961326,DN961328,DN961335,DN961337-DN961339,DN961341,DN961343,DN961344,DN961348,DN961350-DN961353,DN961356,DN961359,DN961360,DN961362,DN961366,DN961370,DN961371,DN961375,DN961376,DN961378,DN961380,DN961385,DN961386,DN961390,DN961392,DN961393,DN961398-DN961402,DN961405-DN961407,DN961417,DN961420-DN961425,DN961428,DN961429,DN961432,DN961433,DN961435,DN961437,DN961439,DN961442,DN961448,DN961452-DN961457,DN961460-DN961462,DN961465,DN961466,DN961469,DN961470,DN961477-DN961479,DN961482-DN961495,DN961497,DN961498,DN961502-DN961504,DN961508,DN961514,DN961519,DN961521,DN961522,DN961524,DN961525,DN961527,DN961532,DN961533,DN961536,DN961538,DN961542,DN961543,DN961545,DN961547,DN961553,DN961555,DN961558-DN961560,DN961562,DN961567-DN961571,DN961574,DN961576-DN961578,DN961580,DN961584,DN961585,DN961587,DN961588,DN961592,DN961597,DN961600,DN961601,DN961603,DN961605,DN961606,DN961609,DN961613,DN961614,DN961616,DN961619,DN961624,DN961625,DN961628-DN961634,DN961637,DN961639,DN961641,DN961642,DN961645,DN961649,DN961650,DN961653-DN961658,DN961661,DN961663,DN961664,DN961666,DN961668,DN961669,DN961671,DN961682-DN961686,DN961688,DN961692-DN961701,DN961705,DN961706,DN961708,DN961714,DN961717-DN961719,DN961724,DN961726,DN961727,DN961729,DN961731-DN961736,DN961738,DN961740,DN961743,DN961744,DN961746-DN961749,DN961751-DN961754,DN961757-DN961760,DN961762,DN961764,DN961766-DN961768,DN961772-DN961774,DN961776,DN961777,DN961779,DN961781,DN961784,DN961786,DN961788,DN961789,DN961793,DN961794,DN961798,DN961801-DN961805,DN961807,DN961808,DN961810,DN961811,DN961813,DN961814,DN961816-DN961820,DN961826,DN961828,DN961830,DN961832-DN961836,DN961838-DN961840,DN961844,DN961846,DN961847,DN961849,DN961852,DN961857,DN961860,DN961861,DN961863-DN961870,DN961873,DN961874,DN961878,DN961881,DN961884,DN961888,DN961891,DN961894,DN961897,DN961900-DN961902,DN961907-DN961910,DN961914,DN961916,DN961918,DN961920-DN961925,DN961929,DN961931,DN961940,DN961943-DN961945,DN961950,DN961952,DN961959,DN961961,DN961966,DN961967,DN961970,DN961972,DN961974,DN961980,DN961982,DN961985,DN961987,DN961991,DN961992,DN962004,DN962011,DN962012,DN962017,DN962021,DN962025,DN962034,DN962035,DN962039,DN962042,DN962045-DN962048,DN962053,DN962057,DN962059,DN962061,DN962067,DN962069,DN962073,DN962074,DN962077,DN962079-DN962084,DN962086,DN962088,DN962093,DN962096,DN962099,DN962100,DN962104,DN962105,DN962107,DN962114,DN962121,DN962122,DN962124,DN962126,DN962133-DN962135,DN962139,DN962141-DN962143,DN962146,DN962147,DN962152,DN962153,DN962156-DN962158,DN962165,DN962166,DN962171,DN962173,DN962175,DN962177,DN962178,DN962192,DN962194,DN962196,DN962197,DN962201,DN962203,DN962207,DN962208,DN962211,DN962214,DN962215,DN962222-DN962224,DN962228,DN962231,DN962233,DN962236,DN962241,DN962244,DN962247,DN962252,DN962258,DN962264,DN962268-DN962271,DN962276-DN962289,DN962293-DN962298,DN962301-DN962304,DN962307,DN962311,DN962312,DN962315,DN962318,DN962319,DN962324,DN962328,DN962331,DN962334,DN962337,DN962339,DN962347,DN962350,DN962354-DN962356,DN962359,DN962364-DN962366,DN962368,DN962370,DN962372,DN962373,DN962375,DN962380-DN962382,DN962384,DN962386,DN962389,DN962391,DN962393,DN962395,DN962396,DN962398,DN962400,DN962401,DN962403,DN962406-DN962408,DN962412-DN962415,DN962418,DN962423,DN962426,DN962432,DN962435-DN962437,DN962440,DN962441,DN962445,DN962447-DN962453,DN962457-DN962459,DN962463-DN962465,DN962467,DN962474,DN962476-DN962479,DN962482-DN962484,DN962492,DN962494,DN962498-DN962502,DN962504,DN962505,DN962507,DN962510,DN962512,DN962513,DN962517,DN962518,DN962520-DN962523,DN962527,DN962528,DN962533-DN962535,DN962537-DN962542,DN962545,DN962548,DN962549,DN962554,DN962557,DN962562,DN962563,DN962569,DN962572-DN962576,DN962578-DN962580,DN962582,DN962585,DN962586,DN962589,DN962591,DN962597-DN962602,DN962605,DN962606,DN962609,DN962610,DN962612,DN962614-DN962617,DN962619,DN962624-DN962626,DN962631,DN962632,DN962636,DN962644-DN962647,DN962650,DN962652,DN962653,DN962655,DN962657-DN962660,DN962663,DN962664,DN962668,DN962669,DN962671,DN962673,DN962674,DN962677,DN962679-DN962682,DN962684,DN962686,DN962687,DN962690,DN962692-DN962694,DN962699,DN962700,DN962703,DN962704,DN962707,DN962708,DN962711,DN962712,DN962715,DN962718,DN962722,DN962723,DN962725,DN962728,DN962731,DN962732,DN962735,DN962736,DN962739,DN962744-DN962749,DN962756,DN962758,DN962759,DN962761,DN962762,DN962766,DN962770,DN962776,DN962779,DN962781,DN962783,DN962787,DN962788,DN962790,DN962792,DN962793,DN962801,DN962803,DN962809,DN962811,DN962814,DN962815,DN962818-DN962822,DN962830,DN962831,DN962832,DN962834,DN962837,DN962840,DN962841,DN962843,DN962844,DN962852,DN962854,DN962857-DN962862,DN962864,DN962865,DN962867,DN962869,DN962870-DN962877,DN962879-DN962882,DN962884,DN962886,DN962888,DN962893,DN962895,DN962897-DN962900,DN962904,DN962906,DN962909,DN962911,DN962917,DN962922,DN962923,DN962925,DN962927,DN962930-DN962933,DN962935,DN962936,DN962938,DN962939,DN962941,DN962942,DN962945,-DN962948,DN962950,DN962952,DN962956,DN962957,DN962962,DN962968,DN962979,DN962981,DN962982,DN962984,DN962985,DN962987-DN962989,DN962991,DN962992,DN962994,DN962995,DN962997,DN962998,DN963001-DN963015,DN963021,DN963022,DN963025,DN963027,DN963028,DN963032,DN963035,DN963037,DN963045,DN963046,DN963050,DN963052,DN963055,DN963056,DN963060,DN963064,DN963065,DN963069,DN963073,DN963080,DN963085,DN963088,DN963090,DN963092-DN963095,DN963100-DN963103,DN963111,DN963113,DN963114,DN963117,DN963119,DN963120,DN963128,DN963132,DN963138,DN963142,DN963144,DN963147,DN963151,DN963156,DN963160,DN963161,DN963164,DN963167,DN963175,DN963179,DN963180,DN963182,DN963185,DN963190,DN963193,DN963197,DN963203,DN963213-DN963215,DN963217,DN963225,DN963227,DN963228,DN963233,DN963234,DN963237,DN963240,DN963244,DN963249,DN963260,DN963266,DN963267,DN963270-DN963272,DN963278-DN963282,DN963284-DN963286,DN963290,DN963291,DN963294,DN963296,DN963298,DN963299,DN963301,DN963308,DN963311,DN963316,DN963317,DN963319,DN963325,DN963327-DN963329,DN963331,DN963333-DN963335,DN963339-DN963341,DN963343,DN963350,DN963355,DN963357-DN963359,DN963361,DN963362,DN963367,DN963373-DN963377,DN963379,DN963383,DN963386,DN963387,DN963388,DN963394-DN963396,DN963399,DN963401,DN963403,DN963404,DN963406,DN963407,DN963411,DN963414,DN963421,DN963429,DN963431,DN963434,DN963439-DN963442,DN963449,DN963461,DN963464,DN963465,DN963467,DN963477,DN963479-DN963483,DN963490,DN963492,DN963493,DN963497,DN963504,DN963507,DN963508,DN963510-DN963515,DN963521,DN963523,DN963524,DN963529,DN963536,DN963538,DN963539,DN963542,DN963548-DN963550,DN963552,DN963555-DN963558,DN963561,DN963563,DN963565,DN963568,DN963571,DN963578,DN963580,DN963581,DN963584,DN963589,DN963593,DN963595,DN963599,DN963600,DN963602,DN963608,DN963615,DN963620-DN963623,DN963632,DN963633,DN963636,DN963637,DN963644-DN963648,DN963650-DN963652,DN963655,DN963657,DN963658,DN963660,DN963663,DN963667,DN963672,DN963674,DN963676,DN963683,DN963685-DN963690,DN963692,DN963696,DN963699,DN963700,DN963702,DN963703,DN963712,DN963715,DN963720,DN963721,DN963724-DN963726,DN963729,DN963731,DN963733,DN963735,DN963736,DN963737,DN963741,DN963743,DN963744,DN963749,DN963750,DN963755,DN963764,DN963766,DN963774,DN963780,DN963782,DN963784-DN963786,DN963795,DN963799,DN963800,DN963802,DN963806,DN963808,DN963810,DN963813,DN963821,DN963825,DN963826,DN963830-DN963832,DN963835,DN963838,DN963839,DN963842,DN963844-DN963846,DN963852,DN963853,DN963856-DN963858,DN963860,DN963863-DN963866,DN963873,DN963877,DN963879,DN963881,DN963888,DN963889,DN963891,DN963895,DN963898,DN963899,DN963903,DN963905,DN963913,DN963917,DN963920,DN963921,DN963923,DN963929,DN963932,DN963935-DN963940,DN963943,DN963945,DN963946,DN963948,DN963954,DN963955,DN963957,DN963966,DN963970,DN963973,DN963977,DN963978,DN963988,DN963989,DN963993,DN963999,DN964000,DN964003,DN964004,DN964006,DN964013,DN964015,DN964017,DN964019,DN964024,DN964027,DN964031,DN964033,DN964036,DN964038,DN964043,DN964048-DN964052,DN964057-DN964062,DN964068,DN964071,DN964073,DN964078,DN964080,DN964081,DN964083,DN964089,DN964092,DN964093,DN964098-DN964100,DN964103,DN964108,DN964109,DN964112,DN964115,DN964118,DN964119,DN964125,DN964130-DN964133,DN964136,DN964144-DN964146,DN964150,DN964154,DN964157,DN964158,DN964162,DN964164,DN964168,DN964173,DN964175,DN964178,DN964185,DN964187,DN964189,DN964194,DN964195,DN964197,DN964201,DN964202,DN964205,DN964206,DN964212,DN964214,DN964217,DN964220-DN964224,DN964230,DN964232,DN964234,DN964235,DN964240,DN964242,DN964243,DN964247,DN964254,DN964256,DN964259,DN964261,DN964267,DN964271,DN964272,DN964277,DN964278,DN964285,DN964286,DN964288,DN964292,DN964295,DN964298,DN964300,DN964303,DN964305,DN964306,DN964308,DN964310,DN964320,DN964334,DN964340,DN964345,DN964347,DN964348,DN964352-DN964355,DN964360,DN964361,DN964362,DN964364,DN964367,DN964372,DN964374,DN964376,DN964378,DN964386-DN964392,DN964394,DN964396,DN964402,DN964404,DN964406,DN964409-DN964416,DN964419,DN964420,DN964422,DN964426,DN964430-DN964436,DN964442,DN964450,DN964452,DN964455,DN964456,DN964462,DN964464,DN964466,DN964469,DN964472,DN964473,DN964478,DN964481,DN964484-DN964488,DN964492-DN964495,DN964498,DN964502-DN964504,DN964507,DN964509,DN964512,DN964514-DN964516,DN964518,DN964521-DN964524,DN964527-DN964529,DN964531,DN964532,DN964534,DN964536,DN964537,DN964538,DN964539,DN964543,DN964544,DN964547,DN964550,DN964551,DN964552,DN964554,DN964559,DN964560,DN964562,DN964563-DN964571,DN964574,DN964576,DN964578,DN964580,DN964582,DN964585-DN964594,DN964598-DN964602,DN964609-DN964612,DN964614-DN964619,DN964622,DN964623,DN964627,DN964628,DN964630,DN964631,DN964633,DN964635,DN964638,DN964640,DN964642,DN964648,DN964651,DN964654,DN964656,DN964658-DN964662,DN964664,DN964666,DN964667,DN964669-DN964675,DN964681,DN964682-DN964684,DN964688,DN964690,DN964693,DN964695-DN964697,DN964699,DN964701,DN964703,DN964709,DN964712,DN964719,DN964722,DN964723,DN964729,DN964732,DN964733,DN964738-DN964742,DN964745,DN964747,DN964749,DN964751,DN964752,DN964756,DN964757,DN964759,DN964760,DN964763,DN964770,DN964771,DN964774-DN964777,DN964781,DN964783-DN964787,DN964791,DN964796,DN964797,DN964800,DN964804,DN964805,DN964807,DN964809-DN964813,DN964816,DN964820-DN964827,DN964831-DN964833,DN964835,DN964837-DN964840,DN964844,DN964849-DN964851,DN964854-DN964856,DN964858,DN964860,DN964862,DN964865,DN964868,DN964870,DN964874,DN964882,DN964885,DN964886,DN964888,DN964889,DN964896,DN964901,DN964903,DN964910-DN964912,DN964916,DN964919,DN964921,DN964926,DN964927,DN964930,DN964932,DN964936,DN964938,DN964939,DN964943,DN964944,DN964947,DN964948,DN964950,DN964953,DN964954,DN964957,DN964959,DN964962,DN964967,DN964972,DN964973,DN964975,DN964979,DN964984,DN964985,DN964994,DN964996,DN965001,DN965004,DN965009,DN965013,DN965017,DN965018-DN965021,DN965029,DN965031,DN965043,DN965055,DN965056,DN965061,DN965063,DN965065-DN965070,DN965080,DN965092,DN965096-DN965099,DN965102,DN965105,DN965108,DN965111,DN965113,DN965119,DN965121,DN965126-DN965128,DN965131,DN965133-DN965135,DN965137,DN965140,DN965142,DN965144,DN965147-DN965152,DN965154,DN965157,DN965158,DN965160,DN965165,DN965168,DN965175,DN965182,DN965183,DN965185,DN965194,DN965197,DN965198,DN965207,DN965212,DN965213,DN965220,DN965224,DN965236,DN965244,DN965245,DN965258,DN965267,DN965269-DN965272,DN965275-DN965278,DN965280,DN965282,DN965284,DN965290,DN965291,DN965293,DN965294,DN965298,DN965301,DN965307,DN965308,DN965313,DN965315,DN965321,DN965323,DN965327,DN965331,DN965335,DN965337,DN965339-DN965343,DN965348,DN965352,DN965353,DN965357-DN965359,DN965368,DN965371,DN965373-DN965377,DN965382,DN965388,DN965390,DN965392,DN965394-DN965396,DN965398,DN965400,DN965402,DN965404,DN965405,DN965410-DN965412,DN965414,DN965421,DN965423-DN965425,DN965428,DN965431-DN965433,DN965435,DN965436,DN965439-DN965445,DN965447,DN965448,DN965450-DN965452,DN965454-DN965456,DN965459,DN965461,DN965463,DN965465,DN965468-DN965470,DN965473,DN965476-DN965485,DN965487-DN965495,DN965497,DN965498,DN965500,DN965503,DN965506,DN965511,DN965513,DN965515,DN965517-DN965519,DN965522,DN965523,DN965525,DN965528,DN965530,DN965532,DN965535,DN965538-DN965540,DN965542,DN965546-DN965551,DN965558,DN965559,DN965561,DN965563,DN965564,DN965568,DN965572,DN965578,DN965579,DN965581-DN965583,DN965595,DN965596,DN965598,DN965600,DN965601,DN965603,DN965604,DN965606,DN965608,DN965609,DN965611,DN965613,DN965621,DN965623,DN965627,DN965632,DN965633,DN965637,DN965647,DN965654,DN965658,DN965660,DN965661,DN965663,DN965665,DN965674,DN965676,DN965679,DN965681,DN965684,DN965686,DN965690,DN965691,DN965694,DN965695,DN965697,DN965699,DN965702,DN965704,DN965706,DN965707,DN965709,DN965719,DN965721,DN965735,DN965736,DN965737,DN965742,DN965743,DN965749-DN965751,DN965760,DN965767,DN965769,DN965774,DN965776,DN965777,DN965786,DN965790,DN965791,DN965793,DN965796,DN965801,DN965804,DN965807-DN965812,DN965815,DN965821,DN965831,DN965833,DN965835,DN965837,DN965838,DN965847,DN965848,DN965850,DN965853,DN965855,DN965858,DN965860,DN965864,DN965866,DN965867,DN965871,DN965873,DN965875,DN965879,DN965882,DN965883,DN965886,DN965889,DN965895-DN965897,DN965900,DN965901,DN965903,DN965905,DN965908,DN965909,DN965914,DN965915,DN965918,DN965919-DN965925,DN965934-DN965938,DN965942,DN965946,DN965959,DN965962,DN965964,DN965966,DN965970,DN965971,DN965975-DN965977,DN965979,DN965980,DN965984,DN965985,DN965987,DN965988,DN965997-DN965999,DN966006,DN966010,DN966013,DN966017,DN966019,DN966021,DN966023,DN966025,DN966031,DN966033,DN966035,DN966041,DN966050,DN966052,DN966054,DN966057,DN966058,DN966064,DN966066,DN966072,DN966073,DN966075,DN966088,DN966089,DN966102,DN966112,DN966116,DN966121,DN966122,DN966127,DN966133,DN966135,DN966140,DN966142,DN966147,DN966157,DN966159,DN966160,DN966161,DN966164,DN966166,DN966170,DN966177,DN966179,DN966180,DN966184,DN966186,DN966191,DN966196,DN966201,DN966203,DN966206,DN966208,DN966211,DN966213,DN966214,DN966216,DN966217,DN966221,DN966223,DN966228,DN966231,DN966233,DN966241,DN966244,DN966250,DN966251,DN966257,DN966259,DN966260,DN966268,DN966272,DN966286,DN966289,DN966291,DN966293,DN966295,DN966297,DN966300,DN966301,DN966309,DN966315,DN966325,DN966330,DN966331-DN966356,DN966358,DN966364,DN966365,DN966367,DN966370,DN966380,DN966383,DN966395,DN966400,DN966405,DN966406,DN966410,DN966414-DN966417,DN966424,DN966427,DN966436,DN966438,DN966451,DN966453,DN966464,DN966467,DN966468,DN966470,DN966472,DN966475,DN966491,DN966496,DN966498,DN966501,DN966504,DN966505,DN966512,DN966513,DN966524,DN966528,DN966530,DN966537,DN966538,DN966540,DN966543,DN966550,DN966552,DN966553,DN966555,DN966556,DN966558,DN966562,DN966566-DN966568,DN966573-DN966576,DN966579,DN966587,DN966591,DN966593,DN966596,DN966599。
本发明的第二个目的是提供一种所述用于检测十字花科植物在胁迫条件下基因表达信息的cDNA芯片的制备方法。
本发明所提供的cDNA芯片的制备方法,包括以下步骤:
1)构建受软腐欧文氏菌(Erwinia carotovora subsp.carotovora)感染大白菜的抑制消减cDNA文库以及大白菜莲座期和结球期幼嫩叶片cDNA文库;
2)自步骤1)中构建的cDNA文库中选取非冗余序列用于制作探针,所述探针序列包括列表中SEQ ID №:1-221的DNA序列,以及3885条具有下述GenBank登录号的DNA序列:
CA991436,CA991442,CA991443,CA991447-CA991450,CA991452-CA991458,CA991460,CA991462,CA991463,CA991465-CA991470,CA991473-CA991482,CA991484-CA991493,CA991495-CA991500,CA991502,CA991503,CA991505,CA991508,CA991510-CA991522,CA991524,CA991525,CA991527,CA991528,CA991530,CA991532,CA991534,CA991536-CA991540,CA991542,CA991544-CA991546,CA991548,CA991549,CA991551-CA991566,CA991568-CA991572,CA991574-CA991577,CA991579,CA991581,CA991582,CA991585-CA991603,CA991606-CA991610,CA991616-CA991654,CA991666-CA991668,CA991671,CA991673-CA991675,CA991678-CA991681,CA991686-CA991693,CA991698-CA991700,CA991705-CA991707,CA991710,CA991714,CA991715,CA991718-CA991720,CA991723,CA991725-CA991727,CA991729-CA991733,CA991735,CA991737-CA991763,CA991770-CA991773,CA991776-CA991778,CA991781,CA991784-CA991790,CA991792,CA991793,CA991795,CA991797-CA991804,CA991806-CA991808,CA991814,CA991817-CA991822,CA991826-CA991832,CA991834,CA991835,CA991839-CA991842,CA991848-CA991852,CA991854,CA991855,CA991857,CA991858-CA991860,CA991866,CA991869,CA991872-CA991875,CA991877,CA991879-CA991882,CA991887,CA991892,CA991895-CA991898,CA991900,CA991901,CA991903-CA991905,CA991907,CA991909,CA991911,CA991912,CA991915-CA991927,CA991930,CA991934,CA991936-CA991944,CA991946-CA991954,CA991960,CA991961,CA991964,CA991966,CA991968-CA991973,CA991976-CA991984,CA991987,CA991989,CA991991-CA991995,CA992000-CA992002,CA992005,CA992007,CA992008,CA992010-CA992012,CA992015,CA992017-CA992025,CA992028,CA992032,CA992033,CA992037,CA992041-CA992045,CA992053-CA992057,CA992061,CA992062,CA992065-CA992069,CA992072-CA992076,CA992080,CA992083,CA992086,CA992088-CA992091,CA992093-CA992097,CA992101,CA992105,CA992114,CA992118,CA992120-CA992122,CA992125,CA992127,CA992130,CA992132,CA992138-CA992146,CA992148,CA992150,CA992155,CA992159,CA992161,CA992163,CA992169-CA992172,CA992175-CA992178,CA992182-CA992192,CA992195,CA992198,CA992202,CA992203,CA992206-CA992208,CA992212,CA992214,CA992215,CA992218,CA992219,CA992221,CA992223-CA992225,CA992227,CA992229,CA992232-CA992234,CA992237,CA992241,CA992247-CA992249,CA992253,CA992254,CA992259-CA992267,CA992272,CA992273,CA992277-CA992283,CA992287-CA992289,CA992292-CA992296,CA992299,CA992303-CA992305,CA992308,CA992310,CA992315,CA992319,CA992324-CA992333,CA992336,CA992338-CA992350,CA992355,CA992356,CA992359-CA992366,CA992373,CA992376,CA992378,CA992380,CA992385-CA992388,CA992391,CA992392,CA992394,CA992396,CA992400,CA992401,CA992406-CA992418,CA992421-CA992424,CA992428,CA992431,CA992432,CA992434,CA992436,CA992439-CA992442,CA992445-CA992448,CA992450,CA992451,CA992458-CA992461,CA992464-CA992473,CA992476-CA992481,CA992484,CA992487,CA992488,CA992492,CA992496,CA992498-CA992504,CA992507,CA992508,CA992510-CA992513,CA992516-CA992524,CA992530-CA992538,CA992540,CA992542-CA992546,CA992553-CA992569,CA992573-CA992579,CA992583-CA992585,DN191370,DN191373-DN191379,DN191385,DN191388-DN191391,DN191394-DN191397,DN191399-DN191401,DN191403,DN191406-DN191413,DN191417,DN191419,DN191422,DN191424,DN191429,DN191430,DN191433-DN191435,DN191438-DN191443,DN191445,DN191450,DN191453,DN191456,DN191458-DN191462,DN191464,DN191467,DN191468,DN191471,DN191473,DN191474,DN191476,DN191479,DN191483,DN191485-DN191488,DN191491,DN191492,DN191497,DN191498,DN191502,DN191503,DN191506,DN191507,DN191510-DN191526,DN191530-DN191532,DN191534-DN191536,DN191538,DN191543,DN191544,DN191546,DN191548-DN191554,DN191556,DN191559-DN191561,DN191563,DN191564,DN191570,DN191572,DN191573,DN191575-DN191577,DN191579,DN191582-DN191586,DN191588-DN191590,DN191594,DN191597,DN191599,DN191603-DN191607,DN191609-DN191618,DN191620-DN191626,DN191629,DN191631-DN191634,DN191636-DN191638,DN191642-DN191644,DN191647,DNl91649,DN191651,DN191653-DN191655,DN191659,DN191662,DN191665,DN191666,DN191670,DN191671,DN191673-DN191677,DN191679-DN191682,DN191685-DN191689,DN191693-DN191696,DN191698,DN191702,N191703,DN191705-DN191712,DN191714-DN191722,DN191724,DN191726,DN191728-DN191730,DN191733,DN191737-DN191739,DN191741,DN191743-DN191745,DN191747-DN191749,DN191751,DN191753,DN191754,DN191757-DN191760,DN191763,DN191765,DN191766,DN191769,DN191771-DN191773,DN191775,DN191777-DN191782,DN191784,DN191787-DN191791,DN191793-DN191795,DN191797-DN191806,DN191808,DN191809,DN191811,DN191813,DN191814,DN191816-DN191822,DN191826,DN191828,DN191832-DN191846,DN191848,DN191850-DN191853,DN191856-DN191863,DN191866,DN191868,DN191869,DN191871,DN191872,DN191875,DN191877-DN191890,DN191891-DN191895,DN191898-DN191900,DN191903-DN191910,DN191914-DN191919,DN191921-DN191926,DN191930-DN191933,DN191937-DN191948,DN191950,DN191953,DN191955-DN191960,DN191962,DN191967,DN191970-DN191972,DN191979,DN191984,DN191986-DN191989,DN191992-DN191994,DN191997,DN192001-DN192005,DN192010,DN192012,DN192017,DN192019-DN192024,DN192026,DN192027,DN192036,DN192037,DN192039-DN192042,DN192044,DN192046,DN192047,DN192049,DN192051,DN192052,DN192054-DN192062,DN192064-DN192067,DN192069-DN192074,DN192076-DN192081,DN192084,DN192085,DN192087,DN192088,DN192090-DN192092,DN192094,DN192095,DN192098-DN192100,DN192103-DN192107,DN192110,DN192112,DN192116,DN192118,DN192120-DN192123,DN192126,DN192128,DN192129,DN192131,DN192133-DN192135,DN192137,DN192138,DN192141,DN192142,DN192145,DN192146,DN192148,DN192150,DN192151,DN192153,DN192157,DN192160,DN192163,DN192164,DN192169,DN192170,DN192172,DN192174,DN192176,DN192179,DN192182-DN192184,DN192186,DN192188-DN192190,DN192192,DN192193,DN192195,DN192199,DN192201-DN192205,DN192207,DN192208,DN192209,DN192211,DN192214,DN192216,DN192219,DN192220,DN192223,DN192225,DN192226,DN192228,DN192230-DN192233,DN192236-DN192238,DN192240,DN192243-DN192247,DN192251-DN192255,DN192257,DN192258,DN192260-DN192263,DN192265-DN192269,DN192271,DN192273,DN192274,DN192276-DN192278,DN192280,DN192281,DN192289,DN192290,DN192293-DN192295,DN192298-DN192300,DN192302-DN192311,DN192314-DN192317,DN192319-DN192321,DN192323,DN192325,DN192327-DN192331, DN192334,DN192336-DN192345,DN192347,DN192348,DN192353,DN192354,DN192357,DN192359,DN192360,DN192362-DN192365,DN192367,DN192370,DN192374,DN192375,DN192377,DN192379,DN192381,DN192382,DN192384,DN192385,DN192389-DN192391,DN192393,DN192398,DN192402,DN192404,DN192406-DN192409,DN192414-DN192416,DN192418,DN192419,DN192421,DN192422,DN192424-DN192426,DN192429-DN192432,DN192434,DN192438-DN192440,DN192447-DN192450,DN192452-DN192454,DN192456,DN192458-DN192461,DN192464,DN192465,DN192467-DN192473,DN192475,DN192479,DN192483,DN192486-DN192489,DN192491,DN192493,DN192495,DN192497,DN192499,DN192501,DN192503-DN192505,DN192507-DN192511,DN192513-DN192515,DN192517,DN192519,DN192521,DN192524-DN192527,DN192529,DN192530,DN192532,DN192536-DN192544,DN192546,DN192547,DN192549,DN192552,DN192554-DN192559,DN192561-DN192563,DN192565,DN192566,DN192568-DN192571,DN192574,DN192576,DN960311-DN960316,DN960318,DN960327,DN960329,DN960332,DN960333,DN960335,DN960341,DN960344,DN960350,DN960353-DN960356,DN960359,DN960373,DN960379-DN960382,DN960384,DN960385,DN960388,DN960389,DN960391,DN960393,DN960394,DN960402-DN960404,DN960407,DN960410,DN960412,DN960419,DN960422,DN960424,DN960426,DN960427,DN960429-DN960431,DN960436,DN960438-DN960440,DN960442,DN960443,DN960446,DN960450,DN960453,DN960458,DN960460-DN960462,DN960465,DN960466,DN960468,DN960471,DN960473,DN960476,DN960478,DN960482,DN960483,DN960485,DN960487,DN960489,DN960491,DN960493,DN960494,DN960496,DN960498-DN960501,DN960505,DN960506,DN960510,DN960511,DN960513-DN960518,DN960521-DN960524,DN960527-DN960529,DN960532,DN960535-DN960538,DN960540,DN960541,DN960542,DN960544,DN960546,DN960547,DN960549,DN960553,DN960555,DN960556,DN960557,DN960559,DN960562,DN960563,DN960565,DN960568,DN960569,DN960571,DN960572,DN960575,DN960577,DN960580,DN960582,DN960584,DN960587,DN960589,DN960592,DN960594,DN960595,DN960597,DN960599,DN960601,DN960603,DN960604,DN960606,DN960611,DN960621,DN960628,DN960633,DN960636,DN960639,DN960643,DN960651,DN960654,DN960657,DN960661,DN960665,DN960666,DN960668,DN960670-DN960673,DN960675,DN960676,DN960685,DN960691,DN960693,DN960694,DN960695,DN960698,DN960700,DN960701,DN960713,DN960716-DN960718,DN960720,DN960727,DN960728,DN960734,DN960736-DN960740,DN960747,DN960750-DN960752,DN960757,DN960759,DN960764,DN960766-DN960770,DN960773,DN960774,DN960776-DN960779,DN960783-DN960785,DN960793-DN960801,DN960803,DN960809,DN960811-DN960813,DN960815-DN960817,DN960821,DN960825,DN960829,DN960832,DN960833,DN960835,DN960837-DN960841,DN960843,DN960844,DN960846-DN960855,DN960858,DN960862,DN960864,DN960868,DN960870-DN960874,DN960876,DN960878,DN960882,DN960883,DN960887,DN960894,DN960896-DN960899,DN960901,DN960903,DN960904,DN960909,DN960915-DN960917,DN960925,DN960930-DN960932,DN960934,DN960939,DN960940,DN960942,DN960944,DN960948,DN960952,DN960956,DN960959,DN960961,DN960964,DN960969,DN960970,DN960974,DN960977,DN960979-DN960982,DN960984,DN960986,DN960987,DN960991,DN960994,DN960995,DN960997,DN961007,DN961017-DN961020,DN961022,DN961024,DN961027,DN961032,DN961034,DN961035,DN961039,DN961042,DN961043,DN961045,DN961047,DN961048,DN961051,DN961053-DN961055,DN961057-DN961060,DN961064,DN961069,DN961072,DN961074,DN961077,DN961079,DN961080,DN961084,DN961086,DN961091,DN961093,DN961097,DN961101,DN961105,DN961107,DN961108,DN961110-DN961112,DN961114,DN961116,DN961120,DN961122-DN961126,DN961129,DN961132-DN961146,DN961148,DN961151,DN961152,DN961154,DN961156,DN961161,DN961164-DN961166,DN961169,DN961170,DN961172-DN961177,DN961180-DN961183,DN961185,DN961186,DN961188,DN961190,DN961194,DN961200,DN961201,DN961203,DN961204,DN961208,DN961211-DN961213,DN961215,DN961217,DN961219-DN961223,DN961225-DN961227,DN961229,DN961231,DN961234,DN961238,DN961240,DN961242,DN961245,DN961247,DN961249,DN961253-DN961255,DN961261,DN961262,DN961265,DN961268,DN961269,DN961271-DN961274,DN961278,DN961279,DN961283-DN961285,DN961293,DN961295-DN961297,DN961300,DN961303,DN961305-DN961308,DN961311,DN961316,DN961318,DN961320-DN961322,DN961324-DN961326,DN961328,DN961335,DN961337-DN961339,DN961341,DN961343,DN961344,DN961348,DN961350-DN961353,DN961356,DN961359,DN961360,DN961362,DN961366,DN961370,DN961371,DN961375,DN961376,DN961378,DN961380,DN961385,DN961386,DN961390,DN961392,DN961393,DN961398-DN961402,DN961405-DN961407,DN961417,DN961420-DN961425,DN961428,DN961429,DN961432,DN961433,DN961435,DN961437,DN961439,DN961442,DN961448,DN961452-DN961457,DN961460-DN961462,DN961465,DN961466,DN961469,DN961470,DN961477-DN961479,DN961482-DN961495,DN961497,DN961498,DN961502-DN961504,DN961508,DN961514,DN961519,DN961521,DN961522,DN961524,DN961525,DN961527,DN961532,DN961533,DN961536,DN961538,DN961542,DN961543,DN961545,DN961547,DN961553,DN961555,DN961558-DN961560,DN961562,DN961567-DN961571,DN961574,DN961576-DN961578,DN961580,DN961584,DN961585,DN961587,DN961588,DN961592,DN961597,DN961600,DN961601,DN961603,DN961605,DN961606,DN961609,DN961613,DN961614,DN961616,DN961619,DN961624,DN961625,DN961628-DN961634,DN961637,DN961639,DN961641,DN961642,DN961645,DN961649,DN961650,DN961653-DN961658,DN961661,DN961663,DN961664,DN961666,DN961668,DN961669,DN961671,DN961682-DN961686,DN961688,DN961692-DN961701,DN961705,DN961706,DN961708,DN961714,DN961717-DN961719,DN961724,DN961726,DN961727,DN961729,DN961731-DN961736,DN961738,DN961740,DN961743,DN961744,DN961746-DN961749,DN961751-DN961754,DN961757-DN961760,DN961762,DN961764,DN961766-DN961768,DN961772-DN961774,DN961776,DN961777,DN961779,DN961781,DN961784,DN961786,DN961788,DN961789,DN961793,DN961794,DN961798,DN961801-DN961805,DN961807,DN961808,DN961810,DN961811,DN961813,DN961814,DN961816-DN961820,DN961826,DN961828,DN961830,DN961832-DN961836,DN961838-DN961840,DN961844,DN961846,DN961847,DN961849,DN961852,DN961857,DN961860,DN961861,DN961863-DN961870,DN961873,DN961874,DN961878,DN961881,DN961884,DN961888,DN961891,DN961894,DN961897,DN961900-DN961902,DN961907-DN961910,DN961914,DN961916,DN961918,DN961920-DN961925,DN961929,DN961931,DN961940,DN961943-DN961945,DN961950,DN961952,DN961959,DN961961,DN961966,DN961967,DN961970,DN961972,DN961974,DN961980,DN961982,DN961985,DN961987,DN961991,DN961992,DN962004,DN962011,DN962012,DN962017,DN962021,DN962025,DN962034,DN962035,DN962039,DN962042,DN962045-DN962048,DN962053,DN962057,DN962059,DN962061,DN962067,DN962069,DN962073,DN962074,DN962077,DN962079-DN962084,DN962086,DN962088,DN962093,DN962096,DN962099,DN962100,DN962104,DN962105,DN962107,DN962114,DN962121,DN962122,DN962124,DN962126,DN962133-DN962135,DN962139,DN962141-DN962143,DN962146,DN962147,DN962152,DN962153,DN962156-DN962158,DN962165,DN962166,DN962171,DN962173,DN962175,DN962177,DN962178,DN962192,DN962194,DN962196,DN962197,DN962201,DN962203,DN962207,DN962208,DN962211,DN962214,DN962215,DN962222-DN962224,DN962228,DN962231,DN962233,DN962236,DN962241,DN962244,DN962247,DN962252,DN962258,DN962264,DN962268-DN962271,DN962276-DN962289,DN962293-DN962298,DN962301-DN962304,DN962307,DN962311,DN962312,DN962315,DN962318,DN962319,DN962324,DN962328,DN962331,DN962334,DN962337,DN962339,DN962347,DN962350,DN962354-DN962356,DN962359,DN962364-DN962366,DN962368,DN962370,DN962372,DN962373,DN962375,DN962380-DN962382,DN962384,DN962386,DN962389,DN962391,DN962393,DN962395,DN962396,DN962398,DN962400,DN962401,DN962403,DN962406-DN962408,DN962412-DN962415,DN962418,DN962423,DN962426,DN962432,DN962435-DN962437,DN962440,DN962441,DN962445,DN962447-DN962453,DN962457-DN962459,DN962463-DN962465,DN962467,DN962474,DN962476-DN962479,DN962482-DN962484,DN962492,DN962494,DN962498-DN962502,DN962504,DN962505,DN962507,DN962510,DN962512,DN962513,DN962517,DN962518,DN962520-DN962523,DN962527,DN962528,DN962533-DN962535,DN962537-DN962542,DN962545,DN962548,DN962549,DN962554,DN962557,DN962562,DN962563,DN962569,DN962572-DN962576,DN962578-DN962580,DN962582,DN962585,DN962586,DN962589,DN962591,DN962597-DN962602,DN962605,DN962606,DN962609,DN962610,DN962612,DN962614-DN962617,DN962619,DN962624-DN962626,DN962631,DN962632,DN962636,DN962644-DN962647,DN962650,DN962652,DN962653,DN962655,DN962657-DN962660,DN962663,DN962664,DN962668,DN962669,DN962671,DN962673,DN962674,DN962677,DN962679-DN962682,DN962684,DN962686,DN962687,DN962690,DN962692-DN962694,DN962699,DN962700,DN962703,DN962704,DN962707,DN962708,DN962711,DN962712,DN962715,DN962718,DN962722,DN962723,DN962725,DN962728,DN962731,DN962732,DN962735,DN962736,DN962739,DN962744-DN962749,DN962756,DN962758,DN962759,DN962761,DN962762,DN962766,DN962770,DN962776,DN962779,DN962781,DN962783,DN962787,DN962788,DN962790,DN962792,DN962793,DN962801,DN962803,DN962809,DN962811,DN962814,DN962815,DN962818-DN962822,DN962830,DN962831,DN962832,DN962834,DN962837,DN962840,DN962841,DN962843,DN962844,DN962852,DN962854,DN962857-DN962862,DN962864,DN962865,DN962867,DN962869,DN962870-DN962877,DN962879-DN962882,DN962884,DN962886,DN962888,DN962893,DN962895,DN962897-DN962900,DN962904,DN962906,DN962909,DN962911,DN962917,DN962922,DN962923,DN962925,DN962927,DN962930-DN962933,DN962935,DN962936,DN962938,DN962939,DN962941,DN962942,DN962945,-DN962948,DN962950,DN962952,DN962956,DN962957,DN962962,DN962968,DN962979,DN962981,DN962982,DN962984,DN962985,DN962987-DN962989,DN962991,DN962992,DN962994,DN962995,DN962997,DN962998,DN963001-DN963015,DN963021,DN963022,DN963025,DN963027,DN963028,DN963032,DN963035,DN963037,DN963045,DN963046,DN963050,DN963052,DN963055,DN963056,DN963060,DN963064,DN963065,DN963069,DN963073,DN963080,DN963085,DN963088,DN963090,DN963092-DN963095,DN963100-DN963103,DN963111,DN963113,DN963114,DN963117,DN963119,DN963120,DN963128,DN963132,DN963138,DN963142,DN963144,DN963147,DN963151,DN963156,DN963160,DN963161,DN963164,DN963167,DN963175,DN963179,DN963180,DN963182,DN963185,DN963190,DN963193,DN963197,DN963203,DN963213-DN963215,DN963217,DN963225,DN963227,DN963228,DN963233,DN963234,DN963237,DN963240,DN963244,DN963249,DN963260,DN963266,DN963267,DN963270-DN963272,DN963278-DN963282,DN963284-DN963286,DN963290,DN963291,DN963294,DN963296,DN963298,DN963299,DN963301,DN963308,DN963311,DN963316,DN963317,DN963319,DN963325,DN963327-DN963329,DN963331,DN963333-DN963335,DN963339-DN963341,DN963343,DN963350,DN963355,DN963357-DN963359,DN963361,DN963362,DN963367,DN963373-DN963377,DN963379,DN963383,DN963386,DN963387,DN963388,DN963394-DN963396,DN963399,DN963401,DN963403,DN963404,DN963406,DN963407,DN963411,DN963414,DN963421,DN963429,DN963431,DN963434,DN963439-DN963442,DN963449,DN963461,DN963464,DN963465,DN963467,DN963477,DN963479-DN963483,DN963490,DN963492,DN963493,DN963497,DN963504,DN963507,DN963508,DN963510-DN963515,DN963521,DN963523,DN963524,DN963529,DN963536,DN963538,DN963539,DN963542,DN963548-DN963550,DN963552,DN963555-DN963558,DN963561,DN963563,DN963565,DN963568,DN963571,DN963578,DN963580,DN963581,DN963584,DN963589,DN963593,DN963595,DN963599,DN963600,DN963602,DN963608,DN963615,DN963620-DN963623,DN963632,DN963633,DN963636,DN963637,DN963644-DN963648,DN963650-DN963652,DN963655,DN963657,DN963658,DN963660,DN963663,DN963667,DN963672,DN963674,DN963676,DN963683,DN963685-DN963690,DN963692,DN963696,DN963699,DN963700,DN963702,DN963703,DN963712,DN963715,DN963720,DN963721,DN963724-DN963726,DN963729,DN963731,DN963733,DN963735,DN963736,DN963737,DN963741,DN963743,DN963744,DN963749,DN963750,DN963755,DN963764,DN963766,DN963774,DN963780,DN963782,DN963784-DN963786,DN963795,DN963799,DN963800,DN963802,DN963806,DN963808,DN963810,DN963813,DN963821,DN963825,DN963826,DN963830-DN963832,DN963835,DN963838,DN963839,DN963842,DN963844-DN963846,DN963852,DN963853,DN963856-DN963858,DN963860,DN963863-DN963866,DN963873,DN963877,DN963879,DN963881,DN963888,DN963889,DN963891,DN963895,DN963898,DN963899,DN963903,DN963905,DN963913,DN963917,DN963920,DN963921,DN963923,DN963929,DN963932,DN963935-DN963940,DN963943,DN963945,DN963946,DN963948,DN963954,DN963955,DN963957,DN963966,DN963970,DN963973,DN963977,DN963978,DN963988,DN963989,DN963993,DN963999,DN964000,DN964003,DN964004,DN964006,DN964013,DN964015,DN964017,DN964019,DN964024,DN964027,DN964031,DN964033,DN964036,DN964038,DN964043,DN964048-DN964052,DN964057-DN964062,DN964068,DN964071,DN964073,DN964078,DN964080,DN964081,DN964083,DN964089,DN964092,DN964093,DN964098-DN964100,DN964103,DN964108,DN964109,DN964112,DN964115,DN964118,DN964119,DN964125,DN964130-DN964133,DN964136,DN964144-DN964146,DN964150,DN964154,DN964157,DN964158,DN964162,DN964164,DN964168,DN964173,DN964175,DN964178,DN964185,DN964187,DN964189,DN964194,DN964195,DN964197,DN964201,DN964202,DN964205,DN964206,DN964212,DN964214,DN964217,DN964220-DN964224,DN964230,DN964232,DN964234,DN964235,DN964240,DN964242,DN964243,DN964247,DN964254,DN964256,DN964259,DN964261,DN964267,DN964271,DN964272,DN964277,DN964278,DN964285,DN964286,DN964288,DN964292,DN964295,DN964298,DN964300,DN964303,DN964305,DN964306,DN964308,DN964310,DN964320,DN964334,DN964340,DN964345,DN964347,DN964348,DN964352-DN964355,DN964360,DN964361,DN964362,DN964364,DN964367,DN964372,DN964374,DN964376,DN964378,DN964386-DN964392,DN964394,DN964396,DN964402,DN964404,DN964406,DN964409-DN964416,DN964419,DN964420,DN964422,DN964426,DN964430-DN964436,DN964442,DN964450,DN964452,DN964455,DN964456,DN964462,DN964464,DN964466,DN964469,DN964472,DN964473,DN964478,DN964481,DN964484-DN964488,DN964492-DN964495,DN964498,DN964502-DN964504,DN964507,DN964509,DN964512,DN964514-DN964516,DN964518,DN964521-DN964524,DN964527-DN964529,DN964531,DN964532,DN964534,DN964536,DN964537,DN964538,DN964539,DN964543,DN964544,DN964547,DN964550,DN964551,DN964552,DN964554,DN964559,DN964560,DN964562,DN964563-DN964571,DN964574,DN964576,DN964578,DN964580,DN964582,DN964585-DN964594,DN964598-DN964602,DN964609-DN964612,DN964614-DN964619,DN964622,DN964623,DN964627,DN964628,DN964630,DN964631,DN964633,DN964635,DN964638,DN964640,DN964642,DN964648,DN964651,DN964654,DN964656,DN964658-DN964662,DN964664,DN964666,DN964667,DN964669-DN964675,DN964681,DN964682-DN964684,DN964688,DN964690,DN964693,DN964695-DN964697,DN964699,DN964701,DN964703,DN964709,DN964712,DN964719,DN964722,DN964723,DN964729,DN964732,DN964733,DN964738-DN964742,DN964745,DN964747,DN964749,DN964751,DN964752,DN964756,DN964757,DN964759,DN964760,DN964763,DN964770,DN964771,DN964774-DN964777,DN964781,DN964783-DN964787,DN964791,DN964796,DN964797,DN964800,DN964804,DN964805,DN964807,DN964809-DN964813,DN964816,DN964820-DN964827,DN964831-DN964833,DN964835,DN964837-DN964840,DN964844,DN964849-DN964851,DN964854-DN964856,DN964858,DN964860,DN964862,DN964865,DN964868,DN964870,DN964874,DN964882,DN964885,DN964886,DN964888,DN964889,DN964896,DN964901,DN964903,DN964910-DN964912,DN964916,DN964919,DN964921,DN964926,DN964927,DN964930,DN964932,DN964936,DN964938,DN964939,DN964943,DN964944,DN964947,DN964948,DN964950,DN964953,DN964954,DN964957,DN964959,DN964962,DN964967,DN964972,DN964973,DN964975,DN964979,DN964984,DN964985,DN964994,DN964996,DN965001,DN965004,DN965009,DN965013,DN965017,DN965018-DN965021,DN965029,DN965031,DN965043,DN965055,DN965056,DN965061,DN965063,DN965065-DN965070,DN965080,DN965092,DN965096-DN965099,DN965102,DN965105,DN965108,DN965111,DN965113,DN965119,DN965121,DN965126-DN965128,DN965131,DN965133-DN965135,DN965137,DN965140,DN965142,DN965144,DN965147-DN965152,DN965154,DN965157,DN965158,DN965160,DN965165,DN965168,DN965175,DN965182,DN965183,DN965185,DN965194,DN965197,DN965198,DN965207,DN965212,DN965213,DN965220,DN965224,DN965236,DN965244,DN965245,DN965258,DN965267,DN965269-DN965272,DN965275-DN965278,DN965280,DN965282,DN965284,DN965290,DN965291,DN965293,DN965294,DN965298,DN965301,DN965307,DN965308,DN965313,DN965315,DN965321,DN965323,DN965327,DN965331,DN965335,DN965337,DN965339-DN965343,DN965348,DN965352,DN965353,DN965357-DN965359,DN965368,DN965371,DN965373-DN965377,DN965382,DN965388,DN965390,DN965392,DN965394-DN965396,DN965398,DN965400,DN965402,DN965404,DN965405,DN965410-DN965412,DN965414,DN965421,DN965423-DN965425,DN965428,DN965431-DN965433,DN965435,DN965436,DN965439-DN965445,DN965447,DN965448,DN965450-DN965452,DN965454-DN965456,DN965459,DN965461,DN965463,DN965465,DN965468-DN965470,DN965473,DN965476-DN965485,DN965487-DN965495,DN965497,DN965498,DN965500,DN965503,DN965506,DN965511,DN965513,DN965515,DN965517-DN965519,DN965522,DN965523,DN965525,DN965528,DN965530,DN965532,DN965535,DN965538-DN965540,DN965542,DN965546-DN965551,DN965558,DN965559,DN965561,DN965563,DN965564,DN965568,DN965572,DN965578,DN965579,DN965581-DN965583,DN965595,DN965596,DN965598,DN965600,DN965601,DN965603,DN965604,DN965606,DN965608,DN965609,DN965611,DN965613,DN965621,DN965623,DN965627,DN965632,DN965633,DN965637,DN965647,DN965654,DN965658,DN965660,DN965661,DN965663,DN965665,DN965674,DN965676,DN965679,DN965681,DN965684,DN965686,DN965690,DN965691,DN965694,DN965695,DN965697,DN965699,DN965702,DN965704,DN965706,DN965707,DN965709,DN965719,DN965721,DN965735,DN965736,DN965737,DN965742,DN965743,DN965749-DN965751,DN965760,DN965767,DN965769,DN965774,DN965776,DN965777,DN965786,DN965790,DN965791,DN965793,DN965796,DN965801,DN965804,DN966807-DN965812,DN965815,DN965821,DN965831,DN965833,DN965635,DN965837,DN965838,DN965847,DN965848,DN965850,DN965853,DN965855,DN965858,DN965860,DN965864,DN965866,DN965867,DN965871,DN965873,DN965875,DN965879,DN965882,DN965883,DN965886,DN965889,DN965895-DN965897,DN965900,DN965901,DN965903,DN965905,DN965908,DN965909,DN965914,DN965915,DN965918,DN965919-DN965925,DN965934-DN965938,DN965942,DN965946,DN965959,DN965962,DN965964,DN965966,DN965970,DN965971,DN965975-DN965977,DN965979,DN965980,DN965984,DN965985,DN965987,DN965988,DN965997-DN965999,DN966006,DN966010,DN966013,DN966017,DN966019,DN966021,DN966023,DN966025,DN966031,DN966033,DN966035,DN966041,DN966050,DN966052,DN966054,DN966057,DN966058,DN966064,DN966066,DN966072,DN966073,DN966075,DN966088,DN966089,DN966102,DN966112,DN966116,DN966121,DN966122,DN966127,DN966133,DN966135,DN966140,DN966142,DN966147,DN966157,DN966159,DN966160,DN966161,DN966164,DN966166,DN966170,DN966177,DN966179,DN966180,DN966184,DN966186,DN966191,DN966196,DN966201,DN966203,DN966206,DN966208,DN966211,DN966213,DN966214,DN966216,DN966217,DN966221,DN966223,DN966228,DN966231,DN966233,DN966241,DN966244,DN966250,DN966251,DN966257,DN966259,DN966260,DN966268,DN966272,DN966286,DN966289,DN966291,DN966293,DN966295,DN966297,DN966300,DN966301,DN966309,DN966315,DN966325,DN966330,DN966331-DN966356,DN966358,DN966364,DN966365,DN966367,DN966370,DN966380,DN966383,DN966395,DN966400,DN966405,DN966406,DN966410,DN966414-DN966417,DN966424,DN966427,DN966436,DN966438,DN966451,DN966453,DN966464,DN966467,DN966468,DN966470,DN966472,DN966475,DN966491,DN966496,DN966498,DN966501,DN966504,DN966505,DN966512,DN966513,DN966524,DN966528,DN966530,DN966537,DN966538,DN966540,DN966543,DN966550,DN966552,DN966553,DN966555,DN966556,DN966558,DN966562,DN966566-DN966568,DN966573-DN966576,DN966579,DN966587,DN966591,DN966593,DN966596,DN966599;
3)利用通用引物PCR扩增目的片段,纯化后的探针浓度应大于100ng/μl;所述通用引物序列为序列表中的SEQ ID №:222和SEQ ID №:223;
4)将步骤3)纯化后的探针干燥后溶于50%DMSO中,探针浓度至少为100ng/μl;
5)点样:将步骤4)的探针溶液点在载玻片上,点制在载玻片上的样品还包括若干个与已知基因没有相似性的基因间序列作为外标,干燥后得到用于检测十字花科植物在胁迫条件下基因表达信息的cDNA芯片。
上述制备方法中,可用Assembly的方法对步骤1)获得的EST进行分析,去除冗余序列。步骤3)制备的探针还包括若干个软腐欧文氏细菌的致病基因片段作为阴性对照,所述软腐欧文氏细菌的致病基因片段优选为具有下述GenBank登录号的DNA片段:AF022772、M36651.1、AF025769.2、L78834.1。步骤5)中每个探针设3个重复,便于进行数据分析;所用载片优选为经过氨基化学修饰的载玻片,更优选为多聚赖氨酸载玻片。
本发明提供了一种用于检测十字花科植物在胁迫条件下基因表达信息的cDNA芯片。该基因芯片以逆境下和特殊发育时期大白菜基因的非冗余cDNA片段作为探针,包含有221个序列表中的cDNA序列以及3885个已经具有GenBank登录号的序列和4个软腐欧文氏细菌致病基因探针及阴性对照,且每个探针设置了3个重复,便于对杂交结果进行统计学分析,提高了检测结果的准确性。
应用该芯片对苗期大白菜对软腐病细菌防御反应基因进行鉴定,结果获取了3152个差异表达基因,重复性高,并构建出了不同基因表达时间和空间上的表达谱。本发明不仅可用于批量检测十字花科植物在生物或非生物胁迫下的基因表达信息、筛选差异基因,还可用于构建十字花科植物不同生长发育阶段、不同组织器官乃至特异细胞中的基因表达谱。应用该芯片可实现基因表达信息的高通量检测,且操作方便、安全,检测结果可靠、有效,可节省大量时间、人力、物力和财力,具有广阔的应用前景。
附图说明
图1为芜菁花叶病毒侵染一周的大白菜材料与对照样品杂交结果的伪色图的部分图片
图2为高抗大白菜接种芜菁花叶病毒后第一周、两周、三周差异表达基因数量的统计结果
具体实施方式
下述实施例中所用方法如无特别说明均为常规方法。所用引物及探针序列均由上海生物工程公司合成。
实施例1、用于检测十字花科植物在胁迫条件下基因表达信息的cDNA芯片的制备
该cDNA芯片的制备方法,包括以下步骤:
1、受软腐病欧文氏病原菌感染大白菜的抑制消减文库以及莲座期和结球期大白菜的cDNA文库的构建
首先用PCR-SlectTM cDNA Subtraction kit(Clontech,CA,USA)并参照试剂盒说明书构建受软腐病欧文氏病原菌感染大白菜叶片的抑制消减文库,用SuperscriptTM Lambda System(GibcoBRL)并参照试剂盒说明书构建莲座期和结球期大白菜的cDNA文库;然后分别从这两个文库中随机挑选克隆进行测序。
2、挑选非冗余序列用于制作探针
对步骤1获得的EST进行Assembly分析,去除冗余序列,获取4106条EST序列可用于制作探针,将探针序列在蛋白及基因(BLASTx)水平上与NCBI(2005年6月)站点上的非冗余数据库进行序列比对,结果与已知防卫病、虫等生物胁迫及干旱、寒冷等非生物胁迫相关基因500多个、还含有大量与已知拟境相关转录因子和信号转导基因、以及在能量代谢、基础代谢中起作用的关键基因。最后选取221条具有序列表中SEQ ID №:1-221 DNA的序列以及3885条下述GenBank登录号对应的DNA序列用作芯片探针:
CA991436,CA991442,CA991443,CA991447-CA991450,CA991452-CA991458,CA991460,CA991462,CA991463,CA991465-CA991470,CA991473-CA991482,CA991484-CA991493,CA991495-CA991500,CA991502,CA991503,CA991505,CA991508,CA991510-CA991522,CA991524,CA991525,CA991527,CA991528,CA991530,CA991532,CA991534,CA991536-CA991540,CA991542,CA991544-CA991546,CA991548,CA991549,CA991551-CA991566,CA991568-CA991572,CA991574-CA991577,CA991579,CA991581,CA991582,CA991585-CA991603,CA991606-CA991610,CA991616-CA991654,CA991666-CA991668,CA991671,CA991673-CA991675,CA991678-CA991681,CA991686-CA991693,CA991698-CA991700,CA991705-CA991707,CA991710,CA991714,CA991715,CA991718-CA991720,CA991723,CA991725-CA991727,CA991729-CA991733,CA991735,CA991737-CA991763,CA991770-CA991773,CA991776-CA991778,CA991781,CA991784-CA991790,CA991792,CA991793,CA991795,CA991797-CA991804,CA991806-CA991808,CA991814,CA991817-CA991822,CA991826-CA991832,CA991834,CA991835,CA991839-CA991842,CA991848-CA991852,CA991854,CA991855,CA991857,CA991858-CA991860,CA991866,CA991869,CA991872-CA991875,CA991877,CA991879-CA991882,CA991887,CA991892,CA991895-CA991898,CA991900,CA991901,CA991903-CA991905,CA991907,CA991909,CA991911,CA991912,CA991915-CA991927,CA991930,CA991934,CA991936-CA991944,CA991946-CA991954,CA991960,CA991961,CA991964,CA991966,CA991968-CA991973,CA991976-CA991984,CA991987,CA991989,CA991991-CA991995,CA992000-CA992002,CA992005,CA992007,CA992008,CA992010-CA992012,CA992015,CA992017-CA992025,CA992028,CA992032,CA992033,CA992037,CA992041-CA992045,CA992053-CA992057,CA992061,CA992062,CA992065-CA992069,CA992072-CA992076,CA992080,CA992083,CA992086,CA992088-CA992091,CA992093-CA992097,CA992101,CA992105,CA992114,CA992118,CA992120-CA992122,CA992125,CA992127,CA992130,CA992132,CA992138-CA992146,CA992148,CA992150,CA992155,CA992159,CA992161,CA992163,CA992169-CA992172,CA992175-CA992178,CA992182-CA992192,CA992195,CA992198,CA992202,CA992203,CA992206-CA992208,CA992212,CA992214,CA992215,CA992218,CA992219,CA992221,CA992223-CA992225,CA992227,CA992229,CA992232-CA992234,CA992237,CA992241,CA992247-CA992249,CA992253,CA992254,CA992259-CA992267,CA992272,CA992273,CA992277-CA992283,CA992287-CA992289,CA992292-CA992296,CA992299,CA992303-CA992305,CA992308,CA992310,CA992315,CA992319,CA992324-CA992333,CA992336,CA992338-CA992350,CA992355,CA992356,CA992359-CA992366,CA992373,CA992376,CA992378,CA992380,CA992385-CA992388,CA992391,CA992392,CA992394,CA992396,CA992400,CA992401,CA992406-CA992418,CA992421-CA992424,CA992428,CA992431,CA992432,CA992434,CA992436,CA992439-CA992442,CA992445-CA992448,CA992450,CA992451,CA992458-CA992461,CA992464-CA992473,CA992476-CA992481,CA992484,CA992487,CA992488,CA992492,CA992496,CA992498-CA992504,CA992507,CA992508,CA992510-CA992513,CA992516-CA992524,CA992530-CA992538,CA992540,CA992542-CA992546,CA992553-CA992569,CA992573-CA992579,CA992583-CA992585,DN191370,DN191373-DN191379,DN191385,DN191388-DN191391,DN191394-DN191397,DN191399-DN191401,DN191403,DN191406-DN191413,DN191417,DN191419,DN191422,DN191424,DN191429,DN191430,DN191433-DN191435,DN191438-DN191443,DN191445,DN191450,DN191453,DN191456,DN191458-DN191462,DN191464,DN191467,DN191468,DN191471,DN191473,DN191474,DN191476,DN191479,DN191483,DN191485-DN191488,DN191491,DN191492,DN191497,DN191498,DN191502,DN191503,DN191506,DN191507,DN191510-DN191526,DN191530-DN191532,DN191534-DN191536,DN191538,DN191543,DN191544,DN191546,DN191548-DN191554,DN191556,DN191559-DN191561,DN191563,DN191564,DN191570,DN191572,DN191573,DN191575-DN191577,DN191579,DN191582-DN191586,DN191588-DN191590,DN191594,DN191597,DN191599,DN191603-DN191607,DN191609-DN191618,DN191620-DN191626,DN191629,DN191631-DN191634,DN191636-DN191638,DN191642-DN191644,DN191647,DN191649,DN191651,DN191653-DN191655,DN191659,DN191662,DN191665,DN191666,DN191670,DN191671,DN191673-DN191677,DN191679-DN191682,DN191685-DN191689,DN191693-DN191696,DN191698,DN191702,DN191703,DN191705-DN191712,DN191714-DN191722,DN191724,DN191726,DN191728-DN191730,DN191733,DN191737-DN191739,DN191741,DN191743-DN191745,DN191747-DN191749,DN191751,DN191753,DN191754,DN191757-DN191760,DN191763,DN191765,DN191766,DN191769,DN191771-DN191773,DN191775,DN191777-DN191782,DN191784,DN191787-DN191791,DN191793-DN191795,DN191797-DN191806,DN191808,DN191809,DN191811,DN191813,DN191814,DN191816-DN191822,DN191826,DN191828,DN191832-DN191846,DN191848,DN191850-DN191853,DN191856-DN191863,DN191866,DN191868,DN191869,DN191871,DN191872,DN191875,DN191877-DN191890,DN191891-DN191895,DN191898-DN191900,DN191903-DN191910,DN191914-DN191919,DN191921-DN191926,DN191930-DN191933,DN191937-DN191948,DN191950,DN191953,DN191955-DN191960,DN191962,DN191967,DN191970-DN191972,DN191979,DN191984,DN191986-DN191989,DN191992-DN191994,DN191997,DN192001-DN192005,DN192010,DN192012,DN192017,DN192019-DN192024,DN192026,DN192027,DN192036,DN192037,DN192039-DN192042,DN192044,DN192046,DN192047,DN192049,DN192051,DN192052,DN192054-DN192062,DN192064-DN192067,DN192069--DN192074,DN192076-DN192081,DN192084,DN192085,DN192087,DN192088,DN192090-DN192092,DN192094,DN192095,DN192098-DN192100,DN192103-DN192107,DN192110,DN192112,DN192116,DN192118,DN192120-DN192123,DN192126,DN192128,DN192129,DN192131,DN192133-DN192135,DN192137,DN192138,DN192141,DN192142,DN192145,DN192146,DN192148,DN192150,DN192151,DN192153,DN192157,DN192160,DN192163,DN192164,DN192169,DN192170,DN192172,DN192174,DN192176,DN192179,DN192182-DN192184,DN192186,DN192188-DN192190,DN192192,DN192193,DN192195,DN192199,DN192201-DN192205,DN192207,DN192208,DN192209,DN192211,DN192214,DN192216,DN192219,DN192220,DN192223,DN192225,DN192226,DN192228,DN192230-DN192233,DN192236-DN192238,DN192240,DN192243-DN192247,DN192251-DN192255,DN192257,DN192258,DN192260-DN192263,DN192265-DN192269,DN192271,DN192273,DN192274,DN192276-DN192278,DN192280,DN192281,DN192289,DN192290,DN192293-DN192295,DN192298-DN192300,DN192302-DN192311,DN192314-DN192317,DN192319-DN192321,DN192323,DN192325,DN192327-DN192331,DN192334,DN192336-DN192345,DN192347,DN192348,DN192353,DN192354,DN192357,DN192359,DN192360,DN192362-DN192365,DN192367,DN192370,DN192374,DN192375,DN192377,DN192379,DN192381,DN192382,DN192384,DN192385,DN192389-DN192391,DN192393,DN192398,DN192402,DN192404,DN192406-DN192409,DN192414-DN192416,DN192418,DN192419,DN192421,DN192422,DN192424-DN192426,DN192429-DN192432,DN192434,DN192438-DN192440,DN192447-DN192450,DN192452-DN192454,DN192456,DN192458-DN192461,DN192464,DN192465,DN192467-DN192473,DN192475,DN192479,DN192483,DN192486-DN192489,DN192491,DN192493,DN192495,DN192497,DN192499,DN192501,DN192503-DN192505,DN192507-DN192511,DN192513-DN192515,DN192517,DN192519,DN192521,DN192524-DN192527,DN192529,DN192530,DN192532,DN192536-DN192544,DN192546,DN192547,DN192549,DN192552,DN192554-DN192559,DN192561-DN192563,DN192565,DN192566,DN192568-DN192571,DN192574,DN192576,DN960311-DN960316,DN960318,DN960327,DN960329,DN960332,DN960333,DN960335,DN960341,DN960344,DN960350,DN960353-DN960356,DN960359,DN960373,DN960379-DN960382,DN960384,DN960385,DN960388,DN960389,DN960391,DN960393,DN960394,DN960402-DN960404,DN960407,DN960410,DN960412,DN960419,DN960422,DN960424,DN960426,DN960427,DN960429-DN960431,DN960436,DN960438-DN960440,DN960442,DN960443,DN960446,DN960450,DN960453,DN960458,DN960460-DN960462,DN960465,DN960466,DN960468,DN960471,DN960473,DN960476,DN960478,DN960482,DN960483,DN960485,DN960487,DN960489,DN960491,DN960493,DN960494,DN960496,DN960498-DN960501,DN960505,DN960506,DN960510,DN960511,DN960513-DN960518,DN960521-DN960524,DN960527-DN960529,DN960532,DN960535-DN960538,DN960540,DN960541,DN960542,DN960544,DN960546,DN960547,DN960549,DN960553,DN960555,DN960556,DN960557,DN960559,DN960562,DN960563,DN960565,DN960568,DN960569,DN960571,DN960572,DN960575,DN960577,DN960580,DN960582,DN960584,DN960587,DN960589,DN960592,DN960594,DN960595,DN960597,DN960599,DN960601,DN960603,DN960604,DN960606,DN960611,DN960621,DN960628,DN960633,DN960636,DN960639,DN960643,DN960651,DN960654,DN960657,DN960661,DN960665,DN960666,DN960668,DN960670-DN960673,DN960675,DN960676,DN960685,DN960691,DN960693,DN960694,DN960695,DN960698,DN960700,DN960701,DN960713,DN960716-DN960718,DN960720,DN960727,DN960728,DN960734,DN960736-DN960740,DN960747,DN960750-DN960752,DN960757,DN960759,DN960764,DN960766-DN960770,DN960773,DN960774,DN960776-DN960779,DN960783-DN960785,DN960793-DN960801,DN960803,DN960809,DN960811-DN960813,DN960815-DN960817,DN960821,DN960825,DN960829,DN960832,DN960833,DN960835,DN960837-DN960841,DN960843,DN960844,DN960846-DN960855,DN960858,DN960862,DN960864,DN960868,DN960870-DN960874,DN960876,DN960878,DN960882,DN960883,DN960887,DN960894,DN960896-DN960899,DN960901,DN960903,DN960904,DN960909,DN960915-DN960917,DN960925,DN960930-DN960932,DN960934,DN960939,DN960940,DN960942,DN960944,DN960948,DN960952,DN960956,DN960959,DN960961,DN960964,DN960969,DN960970,DN960974,DN960977,DN960979-DN960982,DN960984,DN960986,DN960987,DN960991,DN960994,DN960995,DN960997,DN961007,DN961017-DN961020,DN961022,DN961024,DN961027,DN961032,DN961034,DN961035,DN961039,DN961042,DN961043,DN961045,DN961047,DN961048,DN961051,DN961053-DN961055,DN961057-DN961060,DN961064,DN961069,DN961072,DN961074,DN961077,DN961079,DN961080,DN961084,DN961086,DN961091,DN961093,DN961097,DN961101,DN961105,DN961107,DN961108,DN961110-DN961112,DN961114,DN961116,DN961120,DN961122-DN961126,DN961129,DN961132-DN961146,DN961148,DN961151,DN961152,DN961154,DN961156,DN961161,DN961164-DN961166,DN961169,DN961170,DN961172-DN961177,DN961180-DN961183,DN961185,DN961186,DN961188,DN961190,DN961194,DN961200,DN961201,DN961203,DN961204,DN961208,DN961211-DN961213,DN961215,DN961217,DN961219-DN961223,DN961225-DN961227,DN961229,DN961231,DN961234,DN961238,DN961240,DN961242,DN961245,DN961247,DN961249,DN961253-DN961255,DN961261,DN961262,DN961265,DN961268,DN961269,DN961271-DN961274,DN961278,DN961279,DN961283-DN961285,DN961293,DN961295-DN961297,DN961300,DN961303,DN961305-DN961308,DN961311,DN961316,DN961318,DN961320-DN961322,DN961324-DN961326,DN961328,DN961335,DN961337-DN961339,DN961341,DN961343,DN961344,DN961348,DN961350-DN961353,DN961356,DN961359,DN961360,DN961362,DN961366,DN961370,DN961371,DN961375,DN961376,DN961378,DN961380,DN961385,DN961386,DN961390,DN961392,DN961393,DN961398-DN961402,DN961405-DN961407,DN961417,DN961420-DN961425,DN961428,DN961429,DN961432,DN961433,DN961435,DN961437,DN961439,DN961442,DN961448,DN961452-DN961457,DN961460-DN961462,DN961465,DN961466,DN961469,DN961470,DN961477-DN961479,DN961482-DN961495,DN961497,DN961498,DN961502-DN961504,DN961508,DN961514,DN961519,DN961521,DN961522,DN961524,DN961525,DN961527,DN961532,DN961533,DN961536,DN961538,DN961542,DN961543,DN961545,DN961547,DN961553,DN961555,DN961558-DN961560,DN961562,DN961567-DN961571,DN961574,DN961576-DN961578,DN961580,DN961584,DN961585,DN961587,DN961588,DN961592,DN961597,DN961600,DN961601,DN961603,DN961605,DN961606,DN961609,DN961613,DN961614,DN961616,DN961619,DN961624,DN961625,DN961628-DN961634,DN961637,DN961639,DN961641,DN961642,DN961645,DN961649,DN961650,DN961653-DN961658,DN961661,DN961663,DN961664,DN961666,DN961668,DN961669,DN961671,DN961682-DN961686,DN961688,DN961692-DN961701,DN961705,DN961706,DN961708,DN961714,DN961717-DN961719,DN961724,DN961726,DN961727,DN961729,DN961731-DN961736,DN961738,DN961740,DN961743,DN961744,DN961746-DN961749,DN961751-DN961754,DN961757-DN961760,DN961762,DN961764,DN961766-DN961768,DN961772-DN961774,DN961776,DN961777,DN961779,DN961781,DN961784,DN961786,DN961788,DN961789,DN961793,DN961794,DN961798,DN961801-DN961805,DN961807,DN961808,DN961810,DN961811,DN961813,DN961814,DN961816-DN961820,DN961826,DN961828,DN961830,DN961832-DN961836,DN961838-DN961840,DN961844,DN961846,DN961847,DN961849,DN961852,DN961857,DN961860,DN961861,DN961863-DN961870,DN961873,DN961874,DN961878,DN961881,DN961884,DN961888,DN961891,DN961894,DN961897,DN961900-DN961902,DN961907-DN961910,DN961914,DN961916,DN961918,DN961920-DN961925,DN961929,DN961931,DN961940,DN961943-DN961945,DN961950,DN961952,DN961959,DN961961,DN961966,DN961967,DN961970,DN961972,DN961974,DN961980,DN961982,DN961985,DN961987,DN961991,DN961992,DN962004,DN962011,DN962012,DN962017,DN962021,DN962025,DN962034,DN962035,DN962039,DN962042,DN962045-DN962048,DN962053,DN962057,DN962059,DN962061,DN962067,DN962069,DN962073,DN962074,DN962077,DN962079-DN962084,DN962086,DN962088,DN962093,DN962096,DN962099,DN962100,DN962104,DN962105,DN962107,DN962114,DN962121,DN962122,DN962124,DN962126,DN962133-DN962135,DN962139,DN962141-DN962143,DN962146,DN962147,DN962152,DN962153,DN962156-DN962158,DN962165,DN962166,DN962171,DN962173,DN962175,DN962177,DN962178,DN962192,DN962194,DN962196,DN962197,DN962201,DN962203,DN962207,DN962208,DN962211,DN962214,DN962215,DN962222-DN962224,DN962228,DN962231,DN962233,DN962236,DN962241,DN962244,DN962247,DN962252,DN962258,DN962264,DN962268-DN962271,DN962276-DN962289,DN962293-DN962298,DN962301-DN962304,DN962307,DN962311,DN962312,DN962315,DN962318,DN962319,DN962324,DN962328,DN962331,DN962334,DN962337,DN962339,DN962347,DN962350,DN962354-DN962356,DN962359,DN962364-DN962366,DN962368,DN962370,DN962372,DN962373,DN962375,DN962380-DN962382,DN962384,DN962386,DN962389,DN962391,DN962393,DN962395,DN962396,DN962398,DN962400,DN962401,DN962403,DN962406-DN962408,DN962412-DN962415,DN962418,DN962423,DN962426,DN962432,DN962435-DN962437,DN962440,DN962441,DN962445,DN962447-DN962453,DN962457-DN962459,DN962463-DN962465,DN962467,DN912474,DN912471-DN912479,DN912482-DN962484,DN962492,DN962494,DN962498-DN962502,DN962504,DN962505,DN962507,DN962510,DN962512,DN962513,DN962517,DN962518,DN962520-DN962523,DN962527,DN962528,DN962533-DN962535,DN962537-DN962542,DN962545,DN962548,DN962549,DN962554,DN962557,DN962562,DN962563,DN962569,DN962572-DN962576,DN962578-DN962580,DN962582,DN962585,DN962586,DN962589,DN962591,DN962597-DN962602,DN962605,DN962606,DN962609,DN962610,DN962612,DN962614-DN962617,DN962619,DN962624-DN962626,DN962631,DN962632,DN962636,DN962644-DN962647,DN962650,DN962652,DN962653,DN962655,DN962657-DN962660,DN962663,DN962664,DN962668,DN962669,DN962671,DN962673,DN962674,DN962677,DN962679-DN962682,DN962684,DN962686,DN962687,DN962690,DN962692-DN962694,DN962699,DN962700,DN962703,DN962704,DN962707,DN962708,DN962711,DN962712,DN962715,DN962718,DN962722,DN962723,DN962725,DN962728,DN962731,DN962732,DN962735,DN962736,DN962739,DN962744-DN962749,DN962756,DN962758,DN962759,DN962761,DN962762,DN962766,DN962770,DN962776,DN962779,DN962781,DN962783,DN962787,DN962788,DN962790,DN962792,DN962793,DN962801,DN962803,DN962809,DN962811,DN962814,DN962815,DN962818-DN962822,DN962830,DN962831,DN962832,DN962834,DN962837,DN962840,DN962841,DN962843,DN962844,DN962852,DN962854,DN962857-DN962862,DN962864,DN962865,DN962867,DN962869,DN962870-DN962877,DN962879-DN962882,DN962884,DN962886,DN962888,DN962893,DN962895,DN962897-DN962900,DN962904,DN962906,DN962909,DN962911,DN962917,DN962922,DN962923,DN962925,DN962927,DN962930-DN962933,DN962935,DN962936,DN962938,DN962939,DN962941,DN962942,DN962945,-DN962948,DN962950,DN962952,DN962956,DN962957,DN962962,DN962968,DN962979,DN962981,DN962982,DN962984,DN962985,DN962987-DN962989,DN962991,DN962992,DN962994,DN962995,DN962997,DN962998,DN963001-DN963015,DN963021,DN963022,DN963025,DN963027,DN963028,DN963032,DN963035,DN963037,DN963045,DN963046,DN963050,DN963052,DN963055,DN963056,DN963060,DN963064,DN963065,DN963069,DN963073,DN963080,DN963085,DN963088,DN963090,DN963092-DN963095,DN963100-DN963103,DN963111,DN963113,DN963114,DN963117,DN963119,DN963120,DN963128,DN963132,DN963138,DN963142,DN963144,DN963147,DN963151,DN963156,DN963160,DN963161,DN963164,DN963167,DN963175,DN963179,DN963180,DN963182,DN963185,DN963190,DN963193,DN963197,DN963203,DN963213-DN963215,DN963217,DN963225,DN963227,DN963228,DN963233,DN963234,DN963237,DN963240,DN963244,DN963249,DN963260,DN963266,DN963267,DN963270-DN963272,DN963278-DN963282,DN963284-DN963286,DN963290,DN963291,DN963294,DN963296,DN963298,DN963299,DN963301,DN963308,DN963311,DN963316,DN963317,DN963319,DN963325,DN963327-DN963329,DN963331,DN963333-DN963335,DN963339-DN963341,DN963343,DN963350,DN963355,DN963357-DN963359,DN963361,DN963362,DN963367,DN963373-DN963377,DN963379,DN963383,DN963386,DN963387,DN963388,DN963394-DN963396,DN963399,DN963401,DN963403,DN963404,DN963406,DN963407,DN963411,DN963414,DN963421,DN963429,DN963431,DN963434,DN963439-DN963442,DN963449,DN963461,DN963464,DN963465,DN963467,DN963477,DN963479-DN963483,DN963490,DN963492,DN963493,DN963497,DN963504,DN963507,DN963508,DN963510-DN963515,DN963521,DN963523,DN963524,DN963529,DN963536,DN963538,DN963539,DN963542,DN963548-DN963550,DN963552,DN963555-DN963558,DN963561,DN963563,DN963565,DN963568,DN963571,DN963578,DN963580,DN963581,DN963584,DN963589,DN963593,DN963595,DN963599,DN963600,DN963602,DN963608,DN963615,DN963620-DN963623,DN963632,DN963633,DN963636,DN963637,DN963644-DN963648,DN963650-DN963652,DN963655,DN963657,DN963658,DN963660,DN963663,DN963667,DN963672,DN963674,DN963676,DN963683,DN963685-DN963690,DN963692,DN963696,DN963699,DN963700,DN963702,DN963703,DN963712,DN963715,DN963720,DN963721,DN963724-DN963726,DN963729,DN963731,DN963733,DN963735,DN963736,DN963737,DN963741,DN963743,DN963744,DN963749,DN963750,DN963755,DN963764,DN963766,DN963774,DN963780,DN963782,DN963784-DN963786,DN963795,DN963799,DN963800,DN963802,DN963806,DN963808,DN963810,DN963813,DN963821,DN963825,DN963826,DN963830-DN963832,DN963835,DN963838,DN963839,DN963842,DN963844-DN963846,DN963852,DN963853,DN963856-DN963858,DN963860,DN963863-DN963866,DN963873,DN963877,DN963879,DN963881,DN963888,DN963889,DN963891,DN963895,DN963898,DN963899,DN963903,DN963905,DN963913,DN963917,DN963920,DN963921,DN963923,DN963929,DN963932,DN963935-DN963940,DN963943,DN963945,DN963946,DN963948,DN963954,DN963955,DN963957,DN963966,DN963970,DN963973,DN963977,DN963978,DN963988,DN963989,DN963993,DN963999,DN964000,DN964003,DN964004,DN964006,DN964013,DN964015,DN964017,DN964019,DN964024,DN964027,DN964031,DN964033,DN964036,DN964038,DN964043,DN964048-DN964052,DN964057-DN964062,DN964068,DN964071,DN964073,DN964078,DN964080,DN964081,DN964083,DN964089,DN964092,DN964093,DN964098-DN964100,DN964103,DN964108,DN964109,DN964112,DN964115,DN964118,DN964119,DN964125,DN964130-DN964133,DN964136,DN964144-DN964146,DN964150,DN964154,DN964157,DN964158,DN964162,DN964164,DN964168,DN964173,DN964175,DN964178,DN964185,DN964187,DN964189,DN964194,DN964195,DN964197,DN964201,DN964202,DN964205,DN964206,DN964212,DN964214,DN964217,DN964220-DN964224,DN964230,DN964232,DN964234,DN964235,DN964240,DN964242,DN964243,DN964247,DN964254,DN964256,DN964259,DN964261,DN964267,DN964271,DN964272,DN964277,DN964278,DN964285,DN964286,DN964288,DN964292,DN964295,DN964298,DN964300,DN964303,DN964305,DN964306,DN964308,DN964310,DN964320,DN964334,DN964340,DN964345,DN964347,DN964348,DN964352-DN964355,DN964360,DN964361,DN964362,DN964364,DN964367,DN964372,DN964374,DN964376,DN964378,DN964386-DN964392,DN964394,DN964396,DN964402,DN964404,DN964406,DN964409-DN964416,DN964419,DN964420,DN964422,DN964426,DN964430-DN964436,DN964442,DN964450,DN964452,DN964455,DN964456,DN964462,DN964464,DN964466,DN964469,DN964472,DN964473,DN964478,DN964481,DN964484-DN964488,DN964492-DN964495,DN964498,DN964502-DN964504,DN964507,DN964509,DN964512,DN964514-DN964516,DN964518,DN964521-DN964524,DN964527-DN964529,DN964531,DN964532,DN964534,DN964536,DN964537,DN964538,DN964539,DN964543,DN964544,DN964547,DN964550,DN964551,DN964552,DN964554,DN964559,DN964560,DN964562,DN964563-DN964571,DN964574,DN964576,DN964578,DN964580,DN964582,DN964585-DN964594,DN964598-DN964602,DN964609-DN964612,DN964614-DN964619,DN964622,DN964623,DN964627,DN964628,DN964630,DN964631,DN964633,DN964635,DN964638,DN964640,DN964642,DN964648,DN964651,DN964654,DN964656,DN964658-DN964662,DN964664,DN964666,DN964667,DN964669-DN964675,DN964681,DN964682-DN964684,DN964688,DN964690,DN964693,DN964695-DN964697,DN964699,DN964701,DN964703,DN964709,DN964712,DN964719,DN964722,DN964723,DN964729,DN964732,DN964733,DN964738-DN964742,DN964745,DN964747,DN964749,DN964751,DN964752,DN964756,DN964757,DN964759,DN964760,DN964763,DN964770,DN964771,DN964774-DN964777,DN964781,DN964783-DN964787,DN964791,DN964796,DN964797,DN964800,DN964804,DN964805,DN964807,DN964809-DN964813,DN964816,DN964820-DN964827,DN964831-DN964833,DN964835,DN964837-DN964840,DN964844,DN964849-DN964851,DN964854-DN964856,DN964858,DN964860,DN964862,DN964865,DN964868,DN964870,DN964874,DN964882,DN964885,DN964886,DN964888,DN964889,DN964896,DN964901,DN964903,DN964910-DN964912,DN964916,DN964919,DN964921,DN964926,DN964927,DN964930,DN964932,DN964936,DN964938,DN964939,DN964943,DN964944,DN964947,DN964948,DN964950,DN964953,DN964954,DN964957,DN964959,DN964962,DN964967,DN964972,DN964973,DN964975,DN964979,DN964984,DN964985,DN964994,DN964996,DN965001,DN965004,DN965009,DN965013,DN965017,DN965018-DN965021,DN965029,DN965031,DN965043,DN965055,DN965056,DN965061,DN965063,DN965065-DN965070,DN965080,DN965092,DN965096-DN965099,DN965102,DN965105,DN965108,DN965111,DN965113,DN965119,DN965121,DN965126-DN965128,DN965131,DN965133-DN965135,DN965137,DN965140,DN965142,DN965144,DN965147-DN965152,DN965154,DN965157,DN965158,DN965160,DN965165,DN965168,DN965175,DN965182,DN965183,DN965185,DN965194,DN965197,DN965198,DN965207,DN965212,DN965213,DN965220,DN965224,DN965236,DN965244,DN965245,DN965258,DN965267,DN965269-DN965272,DN965275-DN965278,DN965280,DN965282,DN965284,DN965290,DN965291,DN965293,DN965294,DN965298,DN965301,DN965307,DN965308,DN965313,DN965315,DN965321,DN965323,DN965327,DN965331,DN965335,DN965337,DN965339-DN965343,DN965348,DN965352,DN965353,DN965357-DN965359,DN965368,DN965371,DN965373-DN965377,DN965382,DN965388,DN965390,DN965392,DN965394-DN965396,DN965398,DN965400,DN965402,DN965404,DN965405,DN965410-DN965412,DN965414,DN965421,DN965423-DN965425,DN965428,DN965431-DN965433,DN965435,DN965436,DN965439-DN965445,DN965447,DN965448,DN965450-DN965452,DN965454-DN965456,DN965459,DN965461,DN965463,DN965465,DN965468-DN965470,DN965473,DN965476-DN965485,DN965487-DN965495,DN965497,DN965498,DN965500,DN965503,DN965506,DN965511,DN965513,DN965515,DN965517-DN965519,DN965522,DN965523,DN965525,DN965528,DN965530,DN965532,DN965535,DN965538-DN965540,DN965542,DN965546-DN965551,DN965558,DN965559,DN965561,DN965563,DN965564,DN965568,DN965572,DN965578,DN965579,DN965581-DN965583,DN965595,DN965596,DN965598,DN965600,DN965601,DN965603,DN965604,DN965606,DN965608,DN965609,DN965611,DN965613,DN965621,DN965623,DN965627,DN965632,DN965633,DN965637,DN965647,DN965654,DN965658,DN965660,DN965661,DN965663,DN965665,DN965674,DN965676,DN965679,DN965681,DN965684,DN965686,DN965690,DN965691,DN965694,DN965695,DN965697,DN965699,DN965702,DN965704,DN965706,DN965707,DN965709,DN965719,DN965721,DN965735,DN965736,DN965737,DN965742,DN965743,DN965749-DN965751,DN965760,DN965767,DN965769,DN965774,DN965776,DN965777,DN965786,DN965790,DN965791,DN965793,DN965796,DN965801,DN965804,DN965807-DN965812,DN965815,DN965821,DN965831,DN965833,DN965835,DN965837,DN965838,DN965847,DN965848,DN965850,DN965853,DN965855,DN965858,DN965860,DN965864,DN965866,DN965867,DN965871,DN965873,DN965875,DN965879,DN965882,DN965883,DN965886,DN965889,DN965895-DN965897,DN965900,DN965901,DN965903,DN965905,DN965908,DN965909,DN965914,DN965915,DN965918,DN965919-DN965925,DN965934-DN965938,DN965942,DN965946,DN965959,DN965962,DN965964,DN965966,DN965970,DN965971,DN965975-DN965977,DN965979,DN965980,DN965984,DN965985,DN965987,DN965988,DN965997-DN965999,DN966006,DN966010,DN966013,DN966017,DN966019,DN966021,DN966023,DN966025,DN966031,DN966033,DN966035,DN966041,DN966050,DN966052,DN966054,DN966057,DN966058,DN966064,DN966066,DN966072,DN966073,DN966075,DN966088,DN966089,DN966102,DN966112,DN966116,DN966121,DN966122,DN966127,DN966133,DN966135,DN966140,DN966142,DN966147,DN966157,DN966159,DN966160,DN966161,DN966164,DN966166,DN966170,DN966177,DN966179,DN966180,DN966184,DN966186,DN966191,DN966196,DN966201,DN966203,DN966206,DN966208,DN966211,DN966213,DN966214,DN966216,DN966217,DN966221,DN966223,DN966228,DN966231,DN966233,DN966241,DN966244,DN966250,DN966251,DN966257,DN966259,DN966260,DN966268,DN966272,DN966286,DN966289,DN966291,DN966293,DN966295,DN966297,DN966300,DN966301,DN966309,DN966315,DN966325,DN966330,DN966331-DN966356,DN966358,DN966364,DN966365,DN966367,DN966370,DN966380,DN966383,DN966395,DN966400,DN966405,DN966406,DN966410,DN966414-DN966417,DN966424,DN966427,DN966436,DN966438,DN966451,DN966453,DN966464,DN966467,DN966468,DN966470,DN966472,DN966475,DN966491,DN966496,DN966498,DN966501,DN966504,DN966505,DN966512,DN966513,DN966524,DN966528,DN966530,DN966537,DN966538,DN966540,DN966543,DN966550,DN966552,DN966553,DN966555,DN966556,DN966558,DN966562,DN966566-DN966568,DN966573-DN966576,DN966579,DN966587,DN966591,DN966593,DN966596,DN966599。
3、制备探针、设置重复和对照
在探针的制作过程中,对特异性扩增产物施行特异性和量度检测,并避免交叉污染。具体方法为:首先按与步骤1相同的方法以大白菜受软腐病欧文氏病原菌侵染的叶片为材料构建抑制扣除杂交(SSH)cDNA文库和大白菜莲座期及结球期幼嫩叶片为材料构建cDNA文库。挑出步骤2确定的非冗余序列所对应的克隆,以通用引物p-48(序列表中的序列222)和p-47(序列表中的序列223)为引物,使用rTaq DNA聚合酶(5U/μl,TaKaRa公司)扩增目的片段,对扩增的目的片段产物用1.5%琼脂糖凝胶电泳检测特异性;纯化回收后在相同条件下再次进行电泳检测,并对信号较弱的探针进行定量,使其浓度大于100ng/μl。每张芯片上每个探针设置三个重复,并设四个软腐病菌致病因子相关基因(GenBank登录号:AF022772、M36651.1、AF025769.2、L78834.1)用作阴性对照。
4、点样
将纯化后的探针转入384孔板中,使其干燥后溶于50%DMSO中。使用GenomicInstrumentation Services公司的芯片点样仪(GeneMachines)点制在一张75×25mm、经过多聚赖氨酸修饰的载玻片上。步骤3的软腐欧文氏细菌的四个致病基因片段作为阴性对照。每个基因重复三个点。点样后每张芯片可以完成一对样品的检测,保存在无菌干燥处。
经检测该芯片上的探针量足够,点直径的CV值小于10%,点样数量为3×4128。
实施例2、用本发明的cDNA芯片检测高抗和高感芜菁花叶病毒大白菜自交系(均为F6代)对芜菁花叶病毒反应的差异
用本发明的cDNA芯片检测高抗和高感芜菁花叶病毒大白菜自交系(均为F6代)对芜菁花叶病毒反应的差异,具体实验步骤和结果如下:
1、取材
待高抗和高感芜菁花叶病毒大白菜自交系(均为F6代)植株长到4-5片真叶期时,将保存在-20℃的TuMV病毒感染的叶片用pH7.0、浓度为0.1M的磷酸缓冲液以1∶5(wt/vol)磨碎后,取上清液体接种。在健康的高抗和高感植株新展开的叶片上接种芜菁花叶病毒(TuMV),于接种后第一周、两周、三周等时间点取材。
2、RNA的提取及纯化
提取步骤1不同时间点取材的受芜菁花叶病毒感染的高抗和高感大白菜叶片材料的总RNA;然后采用RNeasy mini spin column试剂盒(Qiagen,Valencia,CA,USA)对提取的总RNA过柱进行纯化;再将纯化的总RNA用分光光度计定量,1.5%甲醛变性琼脂糖凝胶电泳进行质检,检测结果表明获得的总RNA未降解,符合实验要求。
3、样品双链cDNA的及纯化
取步骤2获得的不同时间点取材的受芜菁花叶病毒感染的高抗和高感大白菜叶片的总RNA各10g并以此为模板,以T7-Oligo(dT)15,(5’-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGCGCTTTTTTTTTTTTTTTTV-3’,V可以是G、C或A,上海博亚生物技术有限公司)为引物,用cDNA Synthesis试剂盒(TaKaRa公司)并参照试剂盒说明书进行操作合成双链cDNA。双链合成后,用QIAquick PCRPurification试剂盒(Qiagen公司)对合成的目的产物进行纯化。
4、体外转录合成cRNA及纯化
用T7 RiboMAX Express Large Scale RNA Production System(Promega公司)并参照说明书进行操作以步骤3合成的双链cDNA为模板,体外转录合成cRNA。然后用RNeasy Mini试剂盒(Qiagen公司)对体外转录合成cRNA进行纯化。
5、随机引物反转录
取2g步骤4获得的cRNA为模板,用200u/μl SuperscriptII反转录酶(Invitrogen公司),在9mer的随机引物(9mer Random Primer,上海博亚生物技术有限公司)的引导下进行反转录。反转录结束后,对反转录产物用QIAquick PCR Purification试剂盒(Qiagen公司)进行纯化。
6、用KLENOW酶对cDNA反转录产物进行标记
取1g步骤5获得的cDNA为模板,以9mer的随机引物为引物,采用TaKaRa公司的Random Primer DNA Labeling试剂盒并参照说明书进行操作进行KLENOW酶的标记,再将标记产物用QIAquick PCR Purification试剂盒(Qiagen公司)进行纯化,纯化后抽干。标记过程中,dATP、dGTP和dTTP的使用浓度为120M,dCTP的使用浓度为60M,Cy5-dCTP和Cy3-dCTP的使用浓度为40gM(Amersham Pharmacia Biotech,Inc.,Piscataway,NJ,USA)。
7、杂交与清洗
将步骤6经KLENOW酶标记的DNA溶于30μl杂交液(3×SSC,0.2%SDS,5×Denhart’s,25%甲酰胺)中,于42℃杂交12小时。杂交结束后,先在42℃、含0.2%SDS,2×SSC的溶液中洗5min,然后用0.2×SSC溶液,在室温下洗5min。将玻片甩干后即可用于扫描。
8、芯片扫描
将芯片用ScanArray Express双通道激光扫描仪(Packard Bioscience公司)进行扫描,芜菁花叶病毒侵染一周的大白菜材料与对照样品杂交结果的伪色图的部分图片如图1所示(红色为上调表达基因;绿色为下调表达基因;黄色基因表达变化相当)
9、芯片图像的采集与数据分析
采用GenePix Pro 4.0生物芯片图像分析软件(Axon Instruments公司)对扫描的芯片图像进行分析,把图像信号转化为数字信号,然后对芯片上的原始数据进行下述分析:
1)用线性方法进行归一化:用lowess方法校正由于荧光Cy3和Cy5的标记效率和理化性质的不同及其它系统误差所造成的两种荧光信号强度的差异。
2)对数据进行筛选:去除实验中人为加入的外标、内标、阴性对照以及所有空白及不可靠的点。
3)挑选差异表达基因:用T-test统计学方法结合2倍差异确定差异表达基因。挑选差异表达基因的标准为:(1)统计学意义:对3个有效数值进行统计分析,在95%显著性水准上为显著差异;(2)生物学意义:Ratio平均值≥2为上调表达,Ratio平均值≤0.5为下调表达。Ratio值为抗病和感病自交系的结果。高抗、高感大白菜接种芜菁花叶病毒后第一周、两周、三周差异表达基因数量的统计结果如图2所示,在第一周有51个下调、266个上调;第二周有17个下调、239个上调,第三周有7个下调、1812个上调。最终统计结果表明在三个时间点至少有1792个基因差异表达,其中功能未知基因36%、逆境防卫基因9%、次生代谢途径基因5%、转录相关基因5%、信号转导途径占5%。以上结果与检测软腐病基因表达谱结果均表明本发明的芯片具有足够量的探针,可检测到大量差异表达基因,灵敏度较高,可用于快速、批量检测十字花科植物在特殊发育阶段、生物或非生物胁迫下基因的表达信息。
序列表
<160>223
<210>1
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>1
ctggcatatt gggacatcta gcggtttcag acagtggagt tgtgagtgat cgaatcatat 60
caccacctgc tgcacctgaa tgtaataaca aagagccttc tcatgattta gaatcagaaa 120
aggaagaagt tgatgtggag gaggtttcag tgagttcagc tgtgagatct acacgccaaa 180
ccgaaaggaa agctaggagg gcaaaagggc tagagaaaac tgcatcatgt at 232
<210>2
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>2
aagaggacag cactgcactg tgccatgatc tttgatgctc tcgtgatggc gtttgtcatg 60
taaaaattct cgaccactgg tttgaacggc gttgtgctca ttgtctcctt gcactcaggc 120
ttcaacgatg gcccaaaagc agctggctct cgttcatctg tgtggactag atttggtgcg 180
acgctcttga tcctcgatct aacaccttct attgagttgt atggaagatt cacacc 236
<210>3
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>3
acaaaaccag agcaaagcta agcagttttc aagcaaagca taacatagat tgtttcctaa 60
taataaaaaa gcagacatct tcaaaagaat tttgaatgta taaaagaaga acgatcatag 120
ctttgaggaa acagaccaaa tccataacaa tctgcaagag actaaactga gtcttttagc 180
tcgagcagca accagttttc ttcacagccg atacatcaat atcaatcttc tctc 234
<210>4
<211>231
<212>DNA
<213>人工序列
<220>
<223>
<400>4
acccaaaagg aaccttagag gggaaaggaa attcattgtc tttgtatttg catgcgtccg 60
attacgtgac tggtccaaat acaaaaacat tggccgtctt taaactgcga gtgttggacc 120
aactcaaacg caatcaccat gaaataggcc agcagttctg gtttggctct gatggacaaa 180
agggggaacc caagtttttg gcgctggagg aactgcacaa ggcttcaaac g 231
<210>5
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>5
actctccctt gtaatgttga tcgaaaagag tattcatatt tcttgattgt aaatgcttaa 60
ctctgcatgt gtttccttgt aacgttgggt gaggtatgtc gaacatttga tcgctggata 120
tttgggaacg ttgctttcgg acttgcaagt tgggagacac tccaccagac attcatgatt 180
tggttccacg aaaaacggaa tagaatatcg ttcctgaccg ttcccaagaa cccgat 236
<210>6
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>6
acgtaggagg gatcgatgtt cggtaagtga tctgctggtt ctggtaagca gcgtatacct 60
cgagacggtc gtagtaaacg ccgatcttgt cgttagggtt ccgggaagag agagtgactt 120
ggaagttgga ggtgaggagg ttcggtggat taccggagac gttgaaggcg tagacggtgg 180
cgtcttggag gatgaaacgt ggctttgtag gctggagaat tgcccaaatg ag 232
<210>7
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>7
acctttcatc gcgatacatc gcggataggt ttcttccaga taaagctatc gatcttattg 60
acgaggcggg aagcagagct cgcatccaag cttttagaaa gaaaaaggag gatgcaatct 120
gtatcctttc gaagccaccg gatgattact ggcaagagat cagaactgtt caggccatgc 180
acgaagtggt tctatcaagc aggccgaacc aggaagatgg taataacatt gtag 234
<210>8
<211>239
<212>DNA
<213>人工序列
<220>
<223>
<400>8
acccagagca acacgaagag gctattgtct cccagggtgg tagtgtcatt atttccccat 60
tgggaaagat tctcgccggg ccaaactttg aatcagaggg tctcataaca gctgatcttg 120
gtatttgatc ttcatcttaa ttaggcttta gtgcagaaaa ttatgaaccc ttttacccaa 180
tcattttttt ttcttatcta gatcttggcg atatcgcaag agccaagtta tacttcgat 239
<210>9
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>9
acacgctcgg actcggagaa cataacgtct ccaacaaacc gaatctccta tacgacgccg 60
tttgcaaacc gaacgatctt tacagactca aggacaatcc ctacgcggat catgtagata 120
actacgagaa tcaaacgctc tacgtcctcc accagatcct cgaatcgtgg atctacgctt 180
ctctaagcct cctcaaccga atcgacgaga atatcaatga agagagattt gata 234
<210>10
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>10
actggtcagg taagacgttg gctttcgtgt tgcctatatt ggagtcattg atcaatgggc 60
ctgcgaagag caagaggaag aatggttatg gaaggcctcc tagtgttttg gttcttttac 120
cgaccagaga attggccaag caggtgtttg ctgactttga ggcttacgga ggagcagttg 180
ggttagctgc ttgctgtgtc tatggaggcg atccatacgc acctcaagag cata 234
<210>11
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>11
gacaagaaaa aaaaatcaaa ctcgtcatga acgcgagagc tcttctctac tccccgaacc 60
tccaccctct ctacacttca aatcgtccac ctgagacctc tcctcgcaac cgccggactc 120
taaaacctga tccgaagtcg ttacggatat ggattcacca gcggaacagg tccagcgttt 180
tccgggtgct agttcggagc tccgacaaga gagagagcag taactcgtcg ta 232
<210>12
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>12
gtaaagtcga aacctttttt gtgtgtgttc gtaatctctg tctcactcga tggcagcatc 60
tcacttagtg ttttcgttaa gccgccttcc atcatctact cacggaatca atcgaaagtt 120
tcaacctttt ctcctaactc aaacagtagc tccttctcga gttcgtgctc ttcgttgcca 180
agcaacgcca gattcttccg agccccagga gaaattggcg gtggataatg gctca 235
<210>13
<211>230
<212>DNA
<213>人工序列
<220>
<223>
<400>13
acgagacgat gaggggggac ccgttgccgt atgtgaagag gttggctgag tttatggggt 60
atgggttcac ggaggaggaa gaggaaggag gggttgtgga gagagttgtg aagctttgta 120
gctttgagac tttgaagaat cttgaagcta ataaagggga gaaagatagg gaggatcgtc 180
ctgctgtgta tgcgaacagt gcgtatttta ggaaagggaa ggttggggat 230
<210>14
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>14
acgctcactg acaacaagat ggagtttcaa tgtgcttgca tacgtggtgt agattactct 60
gactggaagt ctgcagacaa cgagcatgca ggttactcca ttgaagttga tggaagtatt 120
ttgaagccaa agatgagggt gagagttgat cctgaacttc ttgaactgac gaagaatggc 180
tatgcaacca aagaagcaaa acgtgcgaat gagttcttcc tctcactggc ggc 233
<210>15
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>15
acggtccatt tgtgttgacc gaacgagaca gtctgcttac gagcttgttc cgtccgttgc 60
gtttaagtga ttgtccgaca agaagtgtgt ctgtaggaga gtcaaagctc tgccatatga 120
atttaccatt ggcatcgtgt attaccatat tgccattctc caagatcttg atccccacgg 180
cacctttgtt agccgtgttc gtttgccaga cgaggcgacc atcggcgtcg gcgag 235
<210>16
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>16
ctcaacttac agcatcacca gtgactgtgt cagctaggag cttagcctca ctcagagctt 60
cgagtgccaa gtttggaact ttgaaaccag gcacccttaa gcagacccag ttccgctctt 120
tcgttgtcag agctgcttct gttgttgctc ctaagtatac ttccattaag ccattgggag 180
atcgagtttt ggtgaagatc aaggaggcag aggagaagac tatgggaggt atc 233
<210>17
<211>231
<212>DNA
<213>人工序列
<220>
<223>
<400>17
acaaagaagt ttgtcacgga gctgatccag gaccacccaa gaaccacgtt atcatgacta 60
gcagcgaccg atacaagact agtgttggtg atgttctgcc caagtccgtt gactggagga 120
aaaaaggcgc agtgactgaa atcaaatatc aaggcgattg caggagttgt tgggctttct 180
cgactgtggg ggcagtggaa ggcttgaaca agattgttac aggagagtta g 231
<210>18
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>18
actcatcatc tttggtcgat acttctttag atctcaccat cggtattact cgtatgcgat 60
ttggacaaaa ttcccccaca agtgctttgg tggaccaatt acacacagtg aacgctgata 120
accagaagct ctctcagatg ctcactttga ggcttgacga ctacctattc gtagggaaac 180
atctgatgga agatgttaac ggcagcaaca acacggccga gagagagcaa acca 234
<210>19
<211>197
<212>DNA
<213>人工序列
<220>
<223>
<400>19
acaacggtga atggaagccc aagaaaatta agaaccctaa ctacaaggga aaatggaagg 60
ctccaatgat tgacaaccct gactttaagg atgacccaga actctatgtc ttccccaaat 120
tgaagtatgt tggagtggag ttgtggcagg tgaaatctgg atcattgttc gacaatgtct 180
tggtctgcga tgacccg 197
<210>20
<211>241
<212>DNA
<213>人工序列
<220>
<223>
<400>20
tttgtttaaa ccccctaagg gggtagtata aaaatgaatg tgttgatgtt taaattatcc 60
aaatattgca atctttattt ttaaaaagcc aaatgaaaca tatgtgtata ctcctcaatt 120
ttattaaaac caccttgtct ggatcaacca attgattatt attgggtttt ggcttccgcg 180
gttgaaattc aagcctcttg ttaaacattt tcattcccgg acagaatttt acattgtatt 240
t 241
<210>21
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>21
cccaagcgat tggtttccta gacaagagtg gatgacctgc caaggctccc tacgaccacc 60
tttggtgatc aaagcgccgg tgaagttagg gagaccatcc aaaccccggt gcaccacggg 120
gtatttcccg taccaagatt ggaaatgagt gtcaaaactt ccatcgccta gatccatatc 180
tcccaaaagg acatgattat ccccaggtaa caacccacct ccggcttggc tcgg 234
<210>22
<211>237
<212>DNA
<213>人工序列
<220>
<223>
<400>22
tttaagtgaa actctatttc tcattactct tcaaatttta caattttttt atgtttttac 60
attttttttt gagataacaa gtttgagact gaacatcata aaggtacaaa gaagttacag 120
gttctcactt gtggaaagca gaaaatacta aaaattacaa aggtacaggt ggctaaagat 180
acaacatact ctgcctagtt aactttgcac ttcaacgagg ctggtgaatg tttcgtg 237
<210>23
<211>239
<212>DNA
<213>人工序列
<220>
<223>
<400>23
acagtgtctg ttacgtatac atatacgaac attgagaaac tcattctgta tattcttgtt 60
tgcaaaaagt tatgtcacag agttggagta gcagggacgt agattcatac aacatatgga 120
actcaatgtt tccttctact gttttccagg aacttcacaa acattagctt ttttgtttta 180
gccaccaatg tgtatcattg gtctattagc tgtctttgca atgtcaagca ttccagcgt 239
<210>24
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>24
tttctatgta aatatattca ctaggaaata aaagattacc ccctatgaaa gtttcctaaa 60
tctgcagata tacaaatcta aactactaag gaaaaattca aagaatgaag gaaactaatt 120
attatatcca ttaaaagaaa aaacaaatca acaaacagtt cttccaagct ccaacttgag 180
attcaaattc tcaaccatcc ccaaactcag atccaaacac gccaccctct tccca 235
<210>25
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>25
accccgaggt gccgcttccg tctccatact gaaacgtgta agcgcagagg ttgttctgaa 60
cagaacagcc tgagtcagag gactgaacac cccagctgca tctttggtca gaacatgaga 120
ttggcgtcgc tgttgccgag ctccctggat caaagtaatt caattgaatt tggagtccac 180
tggtttgagg acagccattg caagaagcac aactaaccca caaaacatca ct 232
<210>26
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>26
aggttttcag ttctcacact gggttttctt ctagacatga gagtatccca tataacaatg 60
aagataatca agaaacaatc ttttttccag agttgcagac tggatgggtt gtgtctattt 120
ggtcggccat gttgcttgtg cggacataat gatccccaca ataactccaa acaaaccaag 180
agcgcttccg aagatctcaa tcacaagaat cttcacaaag agtgtggagt tctgag 236
<210>27
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>27
accttcgtga ctttcttgaa gagcaaagac tccagcgacg gatccgagaa ggccttgctt 60
gatgagcttg aagctctgga gactcatctc aagactcatg acggtccttt catcgccgga 120
gggaaagtca gcgccgtgga tctaagctta gcaccgaagc tttaccatct caaggtcgct 180
cttggacact acaaaagctg gtctgtcccc gagagcttgc ctcatgttca tg 232
<210>28
<211>237
<212>DNA
<213>人工序列
<220>
<223>
<400>28
gaggagtgtt atgctcagct tcatagcgcg gtgggaacac cttgtccggc tcacgacggc 60
cttgactcca acaagattgt atttctatac aaatctggag acaaacacgt tttgggtctc 120
aaacaaaatc tcaaattttc acctgaactt tgtgtttcca aattcttttg gcctatgcct 180
aagttccctc cttttaaggg atttgatcat cctttccctt tacctccacc tttggag 237
<210>29
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>29
accgatctat ctacctcggt gccaaaccga agaagagagt cctcaaagcc agcgatcgtt 60
atcagccgcg tgttggtgac gcgatacctg attctgttga ctggagaaaa gaaggtgccg 120
tcgccgccgc taaagatcag ggaagctgcg ggagttgttg ggcattttca actattggag 180
cggtggaagg aataaacaag atcgtgacag gagatttaat ctctttgtct ga 232
<210>30
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>30
acgaggtgga gatgttctcc attgaggatc atttattctg tattcaagga catcccgagt 60
ataacaaaga gattctccac gagatcgttg atcgtgttct tcgtcttggc ttcatcaagc 120
aagattttgc tgatgcggca aaggcctcga tggagaatag gggagcagac aggaaactct 180
tggagacgat ttgcaagaat ttcctcaaag gcagagttcc agctaattaa ttag 234
<210>31
<211>231
<212>DNA
<213>人工序列
<220>
<223>
<400>31
accagaagtt ggcggcggcg ataatgggaa gcgcggaggc taacgcgaat agaacggcgg 60
caagtcacca cgagacgtgt gactcgctga ctcaggtgtt tatggagtca acgtattacc 120
cgagcctaga cgctgcaaag actttcctag gagagttctg gagcaatttg gtatcccgac 180
actgacacag actaaagtaa aaaacactat ccacagatat tgtaacacta c 231
<210>32
<211>230
<212>DNA
<213>人工序列
<220>
<223>
<400>32
acttgatgca atacctggtg gattacgagg acaagaagtt ccagaatgtg tcaaaagaag 60
gactgcaggt tgggaaagac tcgaaagtga aggagctaaa agaagcattc aaggagttaa 120
ccaagtggtg gaaagagagt ctcgctagtg agaacgtgga cgacgtcaaa atcagtaacc 180
gtttagctga tactccctgt gtagttgtaa catctaagtt tggatggagt 230
<210>33
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>33
acaagttatt gattacgaac atgtaaacct ggatgatgta tatgctgctt ttgatgatcc 60
ggaggaacaa aaagaagcac aggttggaac aagagacacg ggtccactga ctctaaatgc 120
atttgacctt attattctgt ctcaaggcct gaaccttgca actctttttg accgtggaaa 180
ggactccatg aagcatcaga cagggttcat ctcacacaag ccagcaaccg tgg 233
<210>34
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>34
tcctctagaa acttctccca tggcggcttc ttctgctctc tcctcaccag cgcttctctc 60
caacaaccgc tctcctccgt catgcggctc cgtcagacgc tcgatctcct cctccccggt 120
gctcatcgct actcccagat cgtctcgttt gagcttcaca atacctgaga ggagaagcct 180
cgtcgtgaaa tcaaccgtcg gcgaggtgtc ctttcgcgaa ccggcaacat cgggag 236
<210>35
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>35
gtgtatcttt ctccgcctat ctctttctct ccgcggaatc tcttcttctc gtttcgactc 60
cgtacgatcc ccaaagaaaa aaagagatgg cggaagagaa gaaagttgct cctactggtg 120
tctggaccga cgtgaggccc ttcgtcaatg gcggagcctc cggtatgctc gcgacttgcg 180
ttatcccgcc cattgacttg atcaaggtga ggattcaact aggtcaggga tctgc 235
<210>36
<211>231
<212>DNA
<213>人工序列
<220>
<223>
<400>36
actttggact cgaagagagc caagttcccc tcatcatcat ccagactcct gacagcaaga 60
agtatctgaa agcaaacgtt gtggttgacc agattgaacc atggatgaag gacttcaagg 120
acgggaaagt tgccgcccac aaaaaatctc agcctatccc agctgagaac aacgagccag 180
ttaaggttgt tgtagctgag agccttgacg agatggtttt caactctgga a 231
<210>37
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>37
cgctgctctc gttctctttg ctgcttttga agcaccaaca atggtgaaag cgcagaagtt 60
gtgcgagagg tctagtggga catggtcagg agtatgtgga aataacaatg cttgcaagaa 120
ccagcgcatc aaccttgagg gagcacgaca tggatcttgc aactatgttt tcccatatca 180
caggtgtatc tgctacttcc catgttaatc taccaaaact cattggtcta ccaa 234
<210>38
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>38
accctcaaca acatccacta cgacaagccc agccaagcta ggcagtgttt tgtttgcagc 60
aacttgcaca gcgaccgaac ctcccatgct gtgaccaact agcacaatag ctggaggaga 120
gtctccatac aattccttaa tcacagccac aacatcattg ctcattctct cgagcgataa 180
gtccagttca tcctctgaaa cagatttacc gtgccctctc aagtccatcg caac 234
<210>39
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>39
acaacagtat gttcccaagg cataaagaga ggatggacaa gagagtggtg gatcttgcaa 60
gagacgttgc taaagttgaa ctgcctgctt acagacgtca cttggatgtt gtggtggctt 120
gtgaggatga caacgacaat gacgttgaca tccctctggt atcagtctac tacgcctgat 180
cagaatctta acatctccca aaagtcttta tactacagaa cagagttttg ttg 233
<210>40
<211>239
<212>DNA
<213>人工序列
<220>
<223>
<400>40
ccgacgatgg ctatttccat aactcatttc ttctccttct cctcttcatc gctctatccg 60
tctccatatt catcaggaaa atcgagaatc attcccttaa gaaaccttca tctccatggc 120
agaccgcctt catctccatc cttttcgaaa ctaaaccgag gggttcaatt catatatcta 180
tctcctataa ccgtcaacag tcagagaagt cacgtatcat ctctctctgc ccaccaagc 239
<210>41
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>41
actcattcac agccttgtga gacgcgtaag aaaagttaac ctcagggcct aactcaggga 60
cgatgtaatg gtagttggtg tcaaaccact tggtcatttc catagcggga acagaggcgt 120
ttcctctagc catggagaag taaacatcaa gcccaatctc tccaccggtg tatccgtatc 180
tgggtggaac agcaccgagc atggcggtgg tatcaagaac ctgatcgtag tga 233
<210>42
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>42
actttttccc aagaattata ccgatgataa cggagatcag agctactcca gtgatgattt 60
tcactgtcat gagatgagaa gttgtagccg tctgagtagc agtaacgttg ctcgaagaag 120
aggaagaagg agatgcctct gacttcttct tgctggtgcg ttttgttgga gaagaaaagg 180
acaagtcgac gtctcgagat ttaataacta catcctttgc ctcagtttcc ttag 234
<210>43
<211>237
<212>DNA
<213>人工序列
<220>
<223>
<400>43
acgacgttag accgggattg cttgaaatct ttggttaagt tcaccaatga caaagggatt 60
caccttattg ctgatgagat ctatgcagcg actacttttg gtgaatccga gtttatcagc 120
gtcgcagaag taattgagga gatccccgat tgcaaccgcg atttgatcca tattgtctat 180
agtttatcaa aagatatggg cttgcccggt ttgagaatcg gtatcatata ctcttac 237
<210>44
<211>228
<212>DNA
<213>人工序列
<220>
<223>
<400>44
atctccagga ggaagctcag gaccgaggtg gacgtcgagg acgtgaagag ggaggtggcg 60
attatgcagc atttgcctga gagctcgagc attgtcactc tgaaggaggc ttgtgaggac 120
gacaacgctg tgcatttggt gatggagctg tgtgagggcg gcgagctttt cgataggatt 180
gtggctagag ggcattacac ggagcgggcg gcggcgggag tcacgaag 228
<210>45
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>45
ctacagccgc cgtagcagcc gcgtctgcga ttccaatcgc gatcgccttc tcctcttctt 60
catcttcctc ctctaccaaa tctcaatctc ccaatctctc cttcctcccc cgatcgtctc 120
cacgtcgctt ggggctttca cgaagcttcg ccagctctcc gatgacgacc gtccccgctt 180
ccgatcgtaa tctccgtcag gaggatcaag tcatgcctca gcttcttact gagttc 236
<210>46
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>46
acgctcggtc tgacagtgtc ttcaccgctg aatcaatgtc gttcgcaagc ttctcagaca 60
ttgagtttct cgccatcatc ctcatgatca catcgctttg tgctgatgag tccatcaacg 120
accatggtcc aatttcagac atcccaaatg ttgtcaccat ctgcttagcc aaaccggtga 180
tctgttgcaa gtcaccaact gcaccagtag tcacctcggg ctcaccaaag atgac 235
<210>47
<211>237
<212>DNA
<213>人工序列
<220>
<223>
<400>47
acttaacacc ttccttcatt atcaacctca ctctcaacac tttgccttca ttgtcatctg 60
gaatcagctg cgttggaagc acttctatca ccgcatccgc atactgcttt tgcgggtcaa 120
tgaatgcatc gaagtcgggc tttcgtgctt cgatacttgc tttgatgctc tctaagctgt 180
gacctctttc agccatgccc ctctggattt tccaagcgaa cttgacctca ttgctaa 237
<210>48
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>48
gttgagatca tcgctaatga tcaaggtaac agaaccaccc cctcttacgt cgccttcacc 60
gactccgaga ggttgattgg tgatgccgcc aagaatcaag tcgccatgaa ccccatcaac 120
accgtcttcg atgcaaagag attgattggt cggagattca gtgacgcatc agtccaaagc 180
gacatgaagc tatggccttt caagatcatc cctggcccag cagacaagcc aat 233
<210>49
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>49
agccgccttt caccagagtc acacggaaat gatgaatcgt tttaaggtgt ggacatacac 60
cgagggagag gccccactgt tccacgacgg ccctgttaat gacatttacg gtatagaagg 120
acaattcatg gacgaaatgt gcgtggaggg ccctatgagg agccattttc gggcagatca 180
ccctgatgat gctcacgttt atttcgttcc atttagcgtc gccaaggtca tcca 234
<210>50
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>50
cattcatcag atctcttctg ggaatcgagg ataaacatga ttcatttcgt gcttctagtt 60
agtcggcaag gaaaagtgag gctcaccaag tggtattcgc cttatacaca gaaggaaagg 120
tctaaggtca tacgcgaact cagcggtgtc atcctcaacc gaggccccaa gctctgcaat 180
ttcatcgagt ggcgaggata caaggttgtc tacaagaggt atgcaagcct gtat 234
<210>51
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>51
acagtgagct tgttacagtt tgtgatgcta agtgtcttca atgaaacaac ttcagaaacc 60
caataaggta actcatagag atcataacaa tagtctatgt caatctcctg taaactaggc 120
agagctttag agacatctat ttcttcagag tcatagaaaa cctcaccgaa gctacacatg 180
accagaggca acttcttgag actggcgagt tgcaaccggg gaatgtctag caa 233
<210>52
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>52
acggatctat gcttattgat aacttctcta catttatcaa gcaagtgtat gcggttgggg 60
caaggaagat cggtgtgaca tctctgcctc caacgggatg tcttccagca gcaagaaccc 120
ttttcggata ccatgaaaaa ggttgtgttt caagactcaa cacagatgct cagcaattca 180
acaagaagct taacgcagct gcttcaaagc ttcagaagca atactctggt ctaa 234
<210>53
<211>237
<212>DNA
<213>人工序列
<220>
<223>
<400>53
actcctcctt ggccatcgaa cccattgctg tgaggcggtg cttgtgagtt catctctatc 60
tgtgtatgga taaggtcttc ttcctgatta tgcacaaaca ctgcagaagg attatagtag 120
ttataacctg acccaaactg caactcctgg ttggggtttg ctccgtaaag cattggcctt 180
tcgtctacag gcatcatagc ttgtccatca acaggaggaa gataagtata gttggta 237
<210>54
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>54
acacaaactg tttaaacaga cacaatttac acgaganagg aaacaaactt tgtatgtaat 60
ggaaggagag agagagagaa caaacaacca cattataact tggacgcctt cttctgctta 120
tncctagcgg caganaagaa gtaaaccgcc atgttcacaa ggctaagccc agccaaaagc 180
catgaagtag taatcaagat gacccgagtt cagattatcc gatatccaac ct 232
<210>55
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>55
actggccaac ccaccgccac catcaccgcc gaaagtagac gcatctaccg ccaccagtgg 60
agagaagaaa gccgatggtg tagataaggc ggctgctcct gctcctccac ccccggccgc 120
ccctaaagag agctcggttg ctttgaagat cagacttcat tgcgaaggat gtattcagaa 180
aatcaagaaa ataatattga aaattaaagg cgtccaaacg gtggctattg at 232
<210>56
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>56
actgatggat atgcatctcc tgagacctac tatttaggtg caaagaaagc acgagaggtc 60
atctacttag ttctgcgtga tgcatgcgcc agtggtgatc tctcactcat ggaagctatt 120
gatgcagcta aagacatttt ctcacggaat tcaattgcat tttataagct taatctagat 180
gttaactcct ctagtcccca aagaagaatc tcgcttgcac cacagatgaa ggagcc 236
<210>57
<211>201
<212>DNA
<213>人工序列
<220>
<223>
<400>57
acatggacgg tggatctcta gaagactttc tgaagtcagt caaaaccatc cctgagtcct 60
atctttctgc catctttaag caagtgcttc agggactgat ctatcttcat cacgataagc 120
acatcatcca ccgtgacttg aaaccatcga atctgttggt caactacaga ggagaggtca 180
agatcactga ctttggtgtg a 201
<210>58
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>58
catttttagc aaaagtgaca atggcgataa aaccactttc catcttcgtt gtcttcttca 60
tcttcttctt agttatctct gacatgccag agatagaagc caaagatagc aagtgtttga 120
gagaatacgg tggtgatgtt ggcttcggct tctgtgcgcc tcggatattt ccgacgtttt 180
gttacacaag atgccgtgag aacaaggggg ctaaaggtgg aagatgccgt tgggg 235
<210>59
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>59
acctatggta atctcccttc atcttaaggt agaaaacctt ggaatctccg tttgcagaag 60
ccgggataag tctcgtatcc agaagcttaa ggataccgtc gcagattttg gagagctccg 120
tttcgatctt gcttctgtag tcacggatcg tggtgacgtg gtcatcgtta ccgcggctct 180
cttccttctg ttcgatggag gagatgatgc gccacgaggc acgacgagcg ccga 234
<210>60
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>60
attgggccga gtcgcatgct cccggccgcc atggcggccg cgggaattcg attagcgtgg 60
tcgcggccga ggtacatgan aattataaat catcaacatc aaatcatgaa aaaggaaaat 120
tttaaccatc ggccgaaaac attattggac cgacaaaaaa agactantca aacattattg 150
agtgatgggc ctttaaatca ctagtgaatt cgcggccgcc tgcaggtcga cca 233
<210>61
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>61
aaatggggtc gaattttaga gataaggcct ggatttttct ggcgattctt tgttattcat 60
cattgatttg ttcagtaaaa gcaacagtct cttatgatcg taaagctgtg atcatcaatg 120
gacaaagaag aattcttctc tcaggttcca tccactatcc acgaagcacc cctgagatgt 180
ggcctggtct gatacagaaa gccaaagaag gaggcttgga tgttatagag acttat 236
<210>62
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>62
acattctttt tcatttcacc acaacccaaa aatgaattgc tcttcaaatc cccaaaccga 60
ttcgatcacc taactctctc tctcacacgt gccaaaaaac tactcaacct taggtgtgat 120
tccactatca tagaaacctc cgacgacgaa tccaccaccg ttgtttctct gcatctccgc 180
catgtaactc ctcacttccg ccttaaccat ctcatgcacc accgtcataa actcag 236
<210>63
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>63
tctttctttt ttctgttgag gaggagggga attgagacat ctcatatgca ttgctcgcac 60
ttctgaaaga tccgataaac ttggaacccg aatgcctcct gccgaaatgc atttattgtt 120
catctaccga agcttgaacg aatggtgatc cccaatagat cccgggttct tcccatggct 180
tgggctcgtt aaagagactg actgctccca acctccgtgc cgggttgatt cgattt 236
<210>64
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>64
acgaatggca gcttcggtag gtaactatga ttacatcatt gattatgagt tccaaactga 60
tgggctaatc aaagctaagg tgggacttag tggaatccta atggtgaaag ggacatcata 120
tcaaaacaaa aatcaagtga ataaagataa agaaggtaac gaagaagagc ttcacggcac 180
gcttctggct gaaaatataa taggagtaat tcatgatcac tatgtcactt tctac 235
<210>65
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>65
agactgttgg gtcaacgtcg gtggttgcgg tagttttccc gactcatatc tttgtagcta 60
actgcggcga ctccagagcc gttctttgcc gcggcaaaac tgcgcttccg ttgtcgactg 120
accacaaacc ggatagagaa gatgaagcgg cgaggattga agctgccgga gggaaagtga 180
tccggtggaa cggagctcgt gtgttcggtg ttctcgccat gtcaagatct att 233
<210>66
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>66
acggggaaga tggatgtgct gacgtgacaa caagacagaa ctggcagatc cgtggtgttg 60
tgttgcctga tgtgcctgag atcttgaaag gtcttgcttc cgttgggtta acgagtctcc 120
aaagtggaat ggataacgtg aggaaccctg ttgggaatcc tatagctggg attgaccctg 180
atgagatagt cgatacaaga ccttacacga accttctctc gcagtttatt act 233
<210>67
<211>237
<212>DNA
<213>人工序列
<220>
<223>
<400>67
accaaccaat ggcaccaacg acagccacca cacccgccac cactgcatag tttctgtcct 60
tgtcttctga cacttttggc cctgattcag ccgatgctga tgatgttcca ccaccaccac 120
tagccttggc tccacttgct gcttgttctc ccggaccctg tcttgctatc ttctcaagct 180
tctcttgctc cattttcttg atgaaaacat tctcagcagc tttctcctct tcgctga 237
<210>68
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>68
ccgcacacga gagttgaaat atcagtctgt tgcttgtgca gatctaaaag gatgagcgtt 60
gcgaaatctc aggtatggca gccatgcaag aagaagtgtt ccttaaggct gtaacccaat 120
accgatctgt gctttagtat tattagtgcg atatatatat agagagagag atggcttctc 180
gaacaacacc ttcgcgatcc actccttctc gatcgacacc ttcaggtagt agcagt 236
<210>69
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>69
gacaaaaatg gctcaagcaa tgacttcgat ggctggttta cgcggtgcgt ctcaggctct 60
ccagaccaat gactcaaacc gtctgagcat ctctagagtc accgttgggt ctaagagaac 120
cgggcttgtg atcagggctc agcagaacga gtcggtgccg gaaagtagtc gccggtcagt 180
gattgggctc gttgcggctg gtttagtcgg tggctcgttc gttaagtctg cc 232
<210>70
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>70
accgagccac ttgaagcagt ggcgatgatc accgtcttgc ggagaggtgg tgctgacgtg 60
acggtggcgt ctgtcgagga taaagttggc gttgatgctt gtcataacat caagatggtc 120
gctgatactc tcctctccga tatcaccgac tctattttcg accttattgt tctccccgga 180
gggcttcccg gaggcgagac tcttaaaaac tgcaaaccat tagagaacat ggtg 234
<210>71
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>71
gttctgttct gtcggagcat tatatcctcg ggtggagttt cggtgtgaac atggacgctc 60
cttctttgcc cttatcgagg cttccgaagc ttcctcggtt cgtgccgagg aagatttcgg 120
attttttcaa gatcggtatg cctttgatct ctctgttttt gatcttctcc gtgatcttcc 180
tcgtctgctt catcgtgagg cggaggagga agttcgcgga ggagcacgag gagtgg 236
<210>72
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>72
accaagtgaa gtgtcgactc cttctggatg ttgtagtccg caagagtgcg accatcctca 60
agctgctttc cagcaaagat gagacgctgc tggtccggag gaatgccttc cttgtcctgg 120
atcttagcct tgacgttgtc aatggtgtct gagctctcaa cctccaatgt gatagtcttg 180
ccggtaaggg tcttcacaaa aatctgcatg ccaccacgga gcctgaggac taa 233
<210>73
<211>237
<212>DNA
<213>人工序列
<220>
<223>
<400>73
acatccactc ggctaaagtt atccacaggg atctaaagcc tagcaatctt ctcttgaacg 60
ccaactgcga tttaaagatc tgtgattttg gtcttgctag acccacttca gagaacgagt 120
ttatgactga gtatgttgtt accagatggt atagagcacc tgagcttctg ttgaactcat 180
ccgattacac agctgctata gatgtttggt ctgttggttg tatctttatg gagctca 237
<210>74
<211>238
<212>DNA
<213>人工序列
<220>
<223>
<400>74
actgactggt gatgccatgg gtattaaaaa tttcataagc atgaaactcg actactccaa 60
attaaaggaa gaattagata taaatgataa agcctttgaa ggaatgtcag atcttcgatt 120
cctaaaagtc tatggttata gtgatgcatt gcaattaact cgaggtctga actatctacc 180
tcgtaaactt cgattactac agtggagtcg ttttccaatg agatattttc cttctacc 238
<210>75
<211>238
<212>DNA
<213>人工序列
<220>
<223>
<400>75
tttagaactg taaaatgttt atcttccgca aaaggttttt gattacaaga tgcatagttt 60
cagtcattta aaacagaatt aaaagagaag taacttcact aaagatctaa ttttttttat 120
atcatatatc ccttccaaaa gatttttttt cctcttcaag acgattccaa aatgagattt 180
gctactgcgg cgccggcgag atcggcggag gagagagaat gattatgatc tccttcgt 238
<210>76
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>76
acgcgccacc taccattact acaacccggc gcagaacaac tgggatttga gagccgtgag 60
tgcctattgc tccacatggg atgctgataa accatactcg tggcggagca agtatggctg 120
gaccgccttc tgtggaccgg ctggtcctcg tggtcaagct tcttgcggca agtgcttaag 180
ggtgaggaac acaaggacta atgcagctgt aacggtgaga attgtggacc aat 233
<210>77
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>77
acatacttga tgacaactac atggtctgga tgttccccgg gctcgaagag gataccgttg 60
ctagcaacca tatcatccac aacattgctt ttggagatct ccttagatct gaatgtctgt 120
ggagctgaga ggttcattcc atcgttgttc cctaggtgat tgtagctcac tattgaagta 180
ggcttgatgc ctgcaccaac aaggaaatca accaagacag atttcatctt ggtttg 236
<210>78
<211>240
<212>DNA
<213>人工序列
<220>
<223>
<400>78
actatagttt atacaactca tcatgcctgc tgattcataa gttcacatag agaaacagag 60
cactctgttt ttgtttaatc ctaaagcgcg aacacaagat tgttgcattc tcgctccttt 120
atttcagaca aaatgcaaca gagtgattag gtatacagtc tttatatata tacttaggta 180
agcatgcatc agattatagt gcagtttctg cagactgttc aggcttctct acttgcttct 240
<210>79
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>79
actttatcag ccaatgaaac tcttcttcat tctcggaaca accatagtca gtagtccatg 60
catggcctat ggtgaacttg tgaaaccgta gcatatccat gacacccacg tgagccaaag 120
cacaaccata aaggtcgggt ctctgattaa tgcaagcacc gatcaggagt ccaccgttac 180
tgccaccttc gatacacagc ttgctgggtt gtgtataacc agcggacacg agat 234
<210>80
<211>136
<212>DNA
<213>人工序列
<220>
<223>
<400>80
accatccatg gtgatcgccg gagccatcgg aggttaggtt gagagagcag atcggactag 60
gcaaacacgg cgctcttcca ctgaaaatgt cgaggttgct tctctcgaag tagttgtagt 120
cagggcccat cagtcc 136
<210>81
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>81
ccattcatcg gaagtttaga aaccccagtg acgtctagcc cattgatcgc gtggtatctc 60
tccaacctcc cggcttaccg cacggccgtc aaccctctcc tccgtggtgt cgaagtgggt 120
ttggctcatg gtttcctctt agtgggtccg tttgtcaaag caggtccatt aaggaacact 180
gcttacgctg gctccgccgg ctctttggcc gctgctggac ttgtagtcat cctca 235
<210>82
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>82
acttggaaaa caacagtctg gcaagagcac ttttcggtcc tccagagact caaatacggc 60
tagattggcc aacgaggcag aggatttgcg tcggtatagc gagaggatta gcttatctcc 120
acgaggaatc aagactcaag attgtgcaca gagacatcaa agcaactaat gtcttgctgg 180
acacggaact aaatgcaaag atttcggatt tcggtcttgc taagcttgat gaa 233
<210>83
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>83
acataagcaa acggctgcgt ctaggtcaac taaacctccc aaaaccggac aacacttttc 60
tttcgcgtgc gttttaccaa gcccgatgtg aatcaaacct ccaagaacgt ccacacaagc 120
gcctagcttc aacgtgtcga ttgggcaagt ctctggctta ggtggagttg gtgttggcgg 180
tgttacgacc ggtgggcttg gtgttggtgg cggtgggcac ggtgatggcg gt 232
<210>84
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>84
acaaggagtg ggacgaaaac aatcctcagc tagcgacatg caacaaggac acaaagaact 60
taatccaaag aaatactgtc cctcaagaag ttgagcaagg gaaagagatt gtgtttacat 120
atgatgtctc atttaaggag agtgaaatca aatgggcttc tcgttgggac acatacctcc 180
tcatgaacga cgatcaaatc cactggttct ccatcataaa ctcgcttatg atc 233
<210>85
<211>231
<212>DNA
<213>人工序列
<220>
<223>
<400>85
acgccacaaa tgcttgccag ccacatccaa cagccacacc tgacaaaacc ggctgaattc 60
cattgagaac aatagtgata gctaaaaagg gagataaatc cgcaacggcc tcgaccacga 120
taggactatc agtaaacacg tagctgatga catttcgcca agacatgacc acgacggctt 180
cgaaaaccgc taataagaat gatacacttg ttgttactac cgtggagaag g 231
<210>86
<211>231
<212>DNA
<213>人工序列
<220>
<223>
<400>86
agaacatgac gagtccagag acccgagaag gtatcctaca cacagggaag tcttggcgta 60
tcttcgagac ttcgcgagag agttcaaact cgtggagatg gttcggttcg ggacagaagt 120
ggttcgtgtc gagcaggatg gtcggaagtg gaagatccga tcaagaaact ccgatggggt 180
gtccagagat gagatctttg actctgttgt cgtttgtaat ggacactaca c 231
<210>87
<211>231
<212>DNA
<213>人工序列
<220>
<223>
<400>87
acttaagccc gaaaatctcg gagatgatag caaatagtag cggccattgc gcaccaaagc 60
aaaaccctat gatgaccgat gcgacgtaga gtccaccagg gacgttaaaa gcaataagaa 120
ggtgaccggc gcaggacaag aggaggatca gtgtgagcat caaaggcctt gggaatctat 180
atttgatcaa gaagatctca gagaccacac ctgatactac acgaccgaag t 231
<210>88
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>88
actttggtga agcttaagca actgagcagg ctcgatctca gctctaatca gctctccggc 60
gagattccac gtggaattcg tggttggaag aatctgaacg agctcaattt agctaataac 120
catctctccg gcgaagtctc tagagggctc ggagacttgc cggttcttaa ctacctcgat 180
ctctcaaaca atcaattttc cggtgagatt ccaccggagc tccagaatct gaag 234
<210>89
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>89
actccggaaa tcatctctgt aacactccct ggctttaaga ggtttaggct caaaggacgt 60
ttgtatccat gtattgtgcc gtctgaggca ggagaagtcc atggaaaggt gctaatggga 120
ttgacggata aagaactatc aaatttagat gctgttgagg gcaatgaata cgagaaagtg 180
acagttgaag ttgtaaggaa ggaaaattct gcgaagatga tagtgaaaac ata 233
<210>90
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>90
acttgaacaa tatagtcaag ggctgtgttg caagtgttgc tgctaagctt gagatcatgg 60
agccttgttg cagtgtcaaa gataggattg ggtatagtat gatcactgat gctgaggaaa 120
aaggacttat tacacctgga aagagtgttc ttgtggaatc tacaagtgga aacacaggga 180
taggtcttgc ttttatagct gcttcaaagg gatataaact tatcttgaca atgcct 236
<210>91
<211>231
<212>DNA
<213>人工序列
<220>
<223>
<400>91
gagaaattga actcagagct gaggtatctg ctatccaagc gaaaggcaag gaaatgagca 60
aagcagagag tgagactggt gaggaaggtc ctatggtgac agagtcagac atccagcaca 120
ttgtgtcttc atggactggt atccctgtag agaaggtatc aaccgatgag tctgatcgcc 180
tcctcaagat ggaagaaacc cttcacaaac gtgtcatagg ccaagatgaa g 231
<210>92
<211>204
<212>DNA
<213>人工序列
<220>
<223>
<400>92
actgacgaat gtctccatga agccagttca caaagtagag gaaccggtca tcgagggaga 60
tcaagaagtc ggtgataagc cccggcattt ctggaagaat ccagttctct actttcagtg 120
gtttaacttg gataaccacc tcatggcccc atgtttcatc actgttcttg aaaaatcgta 180
tcatattact cgacaaagca cccc 204
<210>93
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>93
tgcggtaatg gaccagacta ctgtggttca ggttgccgtt caggtccttg cactggcacc 60
caaggcggta gtcttggtag cattgtgaca caagctttct ttaacggtat tatcaatcaa 120
gccggtggtg gttgcgccgg gaagaatttc tacacccgtg attctttcat taacgccgca 180
aatactttcc caaactttgc caattcggtt actagacgtg aaattgcaac cgtgt 235
<210>94
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>94
acagatattt tacttgaagt gaaagacaag aagattgtcg gcttccatgg cttcgccgac 60
aatcaactca actctcttgg agcttacttc gctcccatcg cctcaactcc attgaaacca 120
agcaagaagc tacaaggagt aggtggtgat gaaggagctt catgggatga tggtgctttc 180
gacggcgtta agaaaataca agtaggacag aacaatgacg gtgtgtcttt cg 232
<210>95
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>95
acctgcccgc ccgacccgtt taaccagatt cgagaacaga attttcatcc ctccccacgt 60
gttgaaacac gtggcgaaga gaaggttgtg agcagcttct tctcgcgaga tacccaattt 120
ctcagcctca actagaatct cgccggaaga ttctaggaag aactcgtaga gtctctggta 180
atcagacttg actaacgccg acggtaaccg gaaagtatgg agaagaggat cttc 234
<210>96
<211>231
<212>DNA
<213>人工序列
<220>
<223>
<400>96
ttgatcattt cgtattctca taaacctaga aaaccaaact ctttttaata gccatggcag 60
aggaaaccaa gaacgttcac gagcatgagg tgccaaaggt agccaccgag gaatcatcaa 120
cggccaccgg cgaggttaca gaccgtggac tgttcgattt cttggggaag aagaaagatg 180
agacgaaacc agaggagacc atcgactctg agttcgagca aaaggttcac a 231
<210>97
<211>241
<212>DNA
<213>人工序列
<220>
<223>
<400>97
acgtagtatt taatcaaatt attttattca tctttattat gaaagatcga gacatttatt 60
ttctttgttt aacattatta gttaaagata ataatacgga gatgatatga tcaaagatca 120
ttagatgaac ttgaactcga tgaacttcaa cagttgacaa actcgtaatt gacatttaag 180
ttgccacgag cgtatccctg cccgtctgta tcaatctgtt tgaagacggc ctcgtctaag 240
t 241
<210>98
<211>237
<212>DNA
<213>人工序列
<220>
<223>
<400>98
acaaacgacc agttcatagg aatataggat caccatatta aaataaaaaa gaaggaaatt 60
ataaaacatt tttaaaacat gcattcgaac atatcttatt taatattctg atccatatta 120
taatattcga aacgtcccct gaagcttcat cacactgtcg ctcatttcac gctgttgcag 180
ttggtgttgg tgctgattgg gaaaggaaca ctgactccac atgccttggg aattcca 237
<210>99
<211>238
<212>DNA
<213>人工序列
<220>
<223>
<400>99
tagcaatatg gaagttttct gcgagatctt aatagcgatc cttcttccac ctcttggagt 60
ctgcctcaag cgtggctgtt gcactgtaga gttcttgata tgcttggtgt tgactatctt 120
aggctacatc ccagggataa tctatgcgct ttacgtgatc gtgttccaga accgtgaagg 180
ggaaactcag gattacagtg ctccactcaa ctcagcttga gattatctgt cggtccat 238
<210>100
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>100
ttttttaata taaataattt tggtgtaaaa cagtaaaatc tataaggtta caacaacata 60
agaaaaagaa aaacagagca tcatcatatc aaattataca tgtgctttga ctgatctttg 120
ttattcaaaa tcagactaga gcaaagaatc agaaggcaag aaactaaaca tgaccatatc 180
tctaacattc cctttaagac acatgaactt cctcatcacg ccttctctca caaacc 236
<210>101
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>101
acagcagaac aggcttatac gtggagtaag ggacgagcta tctttgcaag tggaagccca 60
tttgcacctg tagagtatga ggggaagacg tttgttcctg gacaggcaaa caatgcttac 120
atattccctg ggtttggact ggggctaata atgtcaggga ccattcgtgt tcatgatgac 180
atgcttctgg cagcttcaga agctttggct gagcagctga aggaagagca ctac 234
<210>102
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>102
ttttcttacg cattctcccg agaaacctca cattctcttc ctcctccaca aaacccggat 60
ctctgcatga taagggaata aaactcgttc ttgatttccc tttttttctt tcctttaatt 120
ttcaagtttt cacctgaaac attcaccgtg gagattctgg catcgggtcg gggcgcaaga 180
ggtgggtttc acggcggaga cagatccata ttcccccttc ttctgagggg atagcc 236
<210>103
<211>220
<212>DNA
<213>人工序列
<220>
<223>
<400>103
accatcacac agtgggatac gcaacaatta tcctatccat tattaacatc tttaaaggat 60
tcgatatctt ggatccagaa gataaatggc gatgggctta tattggaatt ctcatctttc 120
ttggtgcctg cgtcgtgatt cttgagccac ttacttggtt tatcgttatt cgccgtaaga 180
accgtggagg taacacagtt gctgcaccgg tttcaaacaa 220
<210>104
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>104
acacactttt agggaaccca ccatacccaa gaggcgaagg ataagcacca gcttcaacaa 60
tcatatcatg caccgctttg tccatttcat tagtcgtcac agatggttta accaaagttc 120
ctgcaaagtt tagaacccga gcagcgagct cgcaagcagc tctcatcttc acaatgcctt 180
caggaccagg aatctggaac tcgggagata tatcgggtaa cacgccagac tcaa 234
<210>105
<211>237
<212>DNA
<213>人工序列
<220>
<223>
<400>105
acctaatcct tgcattaacc ttatctggac tcttaccatt acactctaag tctccattga 60
tggccctaat ggtggctcca aatccttggt tcagtaccgg actgtctccg accactgcac 120
gagctcttag gctcgctgcg actgtctttg ccctcgagcc acaacggtct tagacaaaat 180
ctcttcaaga tattcacagc gacctctatg gtgatcaatc caaatacata attgcct 237
<210>106
<211>229
<212>DNA
<213>人工序列
<220>
<223>
<400>106
accaaaggag ccagcgatcc caccaaggat agtaaacgga gccatgtcaa gcccgagctt 60
gcagaaagtt tcccacgaca tagagatggg gagaaaacct gtgtggcacg agacggtaga 120
agaagagctc ggtgacttgg aaaaggacat ggaaacaacg gttacatcga gggatttgag 180
cagaatgctt ccaccagaag aatctgaagg gcgtgggatt atgcattca 229
<210>107
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>107
acgtctccgt tcagaacagc cgctacgaat ttctcagctt cttccatcgt catgtcgcct 60
ttaaacgtag caaacttccc tcgtttaggc ttgtatgcta ttagaacctt gtcagatgct 120
ttgaactctg atttgtcaaa tgagctcagg aacgaagatt gtttttccgc gtctacaagg 180
gaatatgaaa ccgcgtcctg agagcctgtt cctgaagctt gtcgtctaga caatga 236
<210>108
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>108
acacgtcata gtcataaacg cgttcgtgtt tctcgaattc accagccttc ttgcggttgt 60
tgccttgcaa ggtctccagc tcctccttcc gcagcttctt aaggggctct ggggttgcgg 120
aaggtatgta ggacttgctg gagaagaata tcctcttggc tgggtcaacg gacttggggg 180
ccacccatga gtggcatgaa aacgtaacgg acccgctggg taactcaagc tcc 233
<210>109
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>109
ggagattcca gtcattgatt ttagtgaact ggatggagaa aacagaacca agactatgtc 60
tcttcttgat catgcatgtg ataagtgggg cttcttcatg gttgataatc atggaattga 120
taaagaattg atggataaag taaaacagct gattaactct cattatgagg agcatttgaa 180
agagaagttt taccagtcag agatggtcaa ggctttgagt gaaggcaaaa cgtc 234
<210>110
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>110
atcaaaccag aagaagaaga aaagagaatc ggacatggct tcgccgagca aacgccgaga 60
gatggacatg atgaaattga tgatgagtga ttacaaagtg gatactgtca acgacgattt 120
acatatgttc tatgtcactt tccatggccc cactgacagt ctttacgaag gaggtgtttg 180
gaagatcaaa gtcgaacttc ctgacgctta tccttacaaa tctccttctg ttg 233
<210>111
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>111
accggttcat gcagtgcttc atgattggct taggcatacc gatggaaagc ttagttttct 60
tggatttgtg aagttgttac atggtgtgtc tagccgcact atcaaagctc attagaaggg 120
tttagacagg tctgaatatg tgtgagattt gtttgagatc tcttctctcc caagagcttt 180
ggaggtttat aatatggctt tgtcttttgt gatgaaagct gagaagaagg aaaaaa 236
<210>112
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>112
acaatcactg ggtttgactc tctctcattg caacctaatg ctggtgcttc tggcgagtat 60
accgggctca tggttatccg cgaatatcac aggtcaagag gagatcatca cagaaatgtg 120
tgtatcatcc ccgtctctgc acatgggaca aaccctgcaa gcgctgctat gtgcggaatg 180
aaaattatta cggttggaac tgatgctaag ggaaatatca acatggagga ggtga 235
<210>113
<211>237
<212>DNA
<213>人工序列
<220>
<223>
<400>113
actccagtat gatctcccca caaatctctg gcaagtcctc tgcctttgga acatcaggag 60
ctaagaaaag acttaaggta tctctccaac tcgcagccgg agagtcatag agatcaacat 120
tactgttata cttgaccttt ttagttttgt ctcggctgta gaacattttc ctcgcttctg 180
gatcttgccc gtgaaaccca cgaatcccag cttccatttc ctccatgaca tggagcg 237
<210>114
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>114
actgcctcga gtgcggcgac gccaccgtta accaccgcgt caccgcccta accatatttt 60
ccggtcagat ctccggtcag atcccgccag aggttggtga cttgtcgtat ctacagaccc 120
ttgtcttccg caaactcact aacctcaccg gtcagatccc acgcaccatc gccaagctca 180
agtatctccg gagcctcagg ctcagctgga cgaatctggc cggtccagtt cct 233
<210>115
<211>237
<212>DNA
<213>人工序列
<220>
<223>
<400>115
acgagaaatt gatgatgcca ttaccccctc gttttagtcc aatttgatgg tatcttccac 60
ttcttcttgc ttctttggag ccgccggaaa atatttgatt ccggtaaggt cacctgcttt 120
gcttataacc tgtagagctt taataagatc ttctcttgag tgagatgcgg atatacaaat 180
ccgagccctg gctagcaaca gaggtgtagc tgggaaacca acaaccacaa ctgccac 237
<210>116
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>116
acttgccttg gtgaaaagct tgcgctagct caaggtctgt tggctgcctt gaaccatctc 60
ctgcgaatgt tcctgctcca tagggacttc ctcctttcac tttctccatc tcgaacattc 120
ccgcaccaaa tgtgtaaccg atcgggacaa agatcatccc gtggtggacc agctgagtta 180
tcgcagtcaa tgcggtggtt tcttggccac caccttgaga gccagtgctg taga 234
<210>117
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>117
acagtgtaaa tcactgtgga gacaattcaa aaacgagaca gaatataccg ttacacaggc 60
tatttctgca caggaagcta accgacggaa taacaactgg ttgccacctc catgggcaat 120
ccttgccctg gtagttcttg gatttaatga gtttatgact ctcttaagaa atcctctatg 180
gctaggtgtt ctgttcgtcg gataccttgt ttccaaagcc ttgtgggtgc agtta 235
<210>118
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>118
acgcatcgag actcgtattc ttcggatttn ggaaagagca tttccttaga aggatttcct 60
ggggagatgt gtcccaccac ccatccttga aggagatcat ggagatcgcg aacatgctga 120
gaaacgccga gagcttccat agccttccaa gcgttgaacc aagtagtaaa tgcgaatcct 180
gtggctctca gcttatctga agattccagc accatgcttc taatcccaag ccgg 234
<210>119
<211>294
<212>DNA
<213>人工序列
<220>
<223>
<400>119
aggcgattca acacgttgat gtagttatct caaccgttgg tctctctcaa ctcatgaacc 60
agctcaatat catctctgct attaaagaat ctggcaagca tattaagaga ttcttgccgt 120
ctgagtttgg caacgatgtg gaccgaacgt tggcgactgg accagcaaag tcggagttcg 180
caatgaaggc tgaggtccgt cgtgctgttg aagctgaagg tgtaccatac acatacgtta 240
tcaacaattg ctttgatggc tatttcttag ccacattagc tcaatgtgaa acca 294
<210>120
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>120
actcaagaag catttgttgg cttcatcgtc cgttgagaag gttgagtggt caaccttaca 60
gaacgaggct aggaagccag tcttagttgt tttccctgct aagagagttg ataacttttc 120
tggattctcc gtaaggataa ttccttcagc gacatcactg tttgatgttg caaagctgag 180
tatgagcaga aacaacgtcc gttctgtaac tgcagatggt gtcgctcagc caa 233
<210>121
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>121
gaagattttg tctttgatga cgctgctttt ttctcaggac tctggtctct agaaccctta 60
aaccaagttc ctaaacagga gcctagttca ccggctcttg atccagattc ctatgtccaa 120
gagtttctgc aaatggaagc agaatcatca tcatcaacaa caacaacaac tacaactaca 180
acatcacctg aggttgaaac tgtctcaaac cggaaaagat caaagagagc tga 233
<210>122
<211>173
<212>DNA
<213>人工序列
<220>
<223>
<400>122
tactcaagga taaccttaaa gagacttgca gagcttttat gcctaagcat tgaggaggcg 60
gagaagcatc tgtcagagat ggtagtgtcg aaagcactga ttgcaaaaat agacagacct 120
tcaggaatag tgtgcttcca ggtctcaaag gacagcaacg agattctcaa ctc 173
<210>123
<211>177
<212>DNA
<213>人工序列
<220>
<223>
<400>123
ggataattca actgaattta tttcttgatc aaattatatt ggaggccaac tcctacaaac 60
tgaaatcaca aaaggaaatg gaaacgtgtg gtttgtccaa aaacataaga accacaactt 120
tatttgataa attgaaataa aatgacaggt ggaagtgaaa tgcatatgat atgtatg 177
<210>124
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>124
tctcggagct ccttcttcac catggcggca ggtctctcaa ccaccgtaac attcaaccct 60
ctccaccgct ctttctcatc ctcctcaagt gtccgcttac accacccaag atccttaacc 120
ggactccctt catctctccg gttcagaggc ttctcggtct gctacgtcgt cgaggagcag 180
aggcagagct ctcccgtcga caacgatgaa agacctgaga gaaccaacgt cata 234
<210>125
<211>178
<212>DNA
<213>人工序列
<220>
<223>
<400>125
tagaacacag tcttcatatc ctttagaggc tgctatatgc agtggagttt ttcctttgga 60
atctgtaatg tcagggctta acttagcctt gagtatctcg tcaagaagag ctgcattgcc 120
tgtagataca acagtgataa gatttgaggc aatgttgggt gaaccatcat cgtcttct 178
<210>126
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>126
tcaaatacgg acctgctacc aagaaggctc gttcagaacc tctttcctta tacgcatgtt 60
tggaagcgtt tctacgtgaa gagcctttag tgcctgacga aatgtggttc tgtccacaat 120
gcaatgaaag aaggcaggca agcaaaaagc tggatttgtg gaggctaccc gaagttcttg 180
tcatacatct gaagagattc tcatacagca gatcaatgaa gcacaagctg gaa 233
<210>127
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>127
tttgggtgat atatgggaaa ctatctccgg cagtgatctt gtattgcttt tgatctctga 60
tgctgctcaa gctgataact atgagaaaat attctcccac atgaaaccaa acagcattct 120
cggtttgtca cacgggttct tactggggca tttacagtca atgggacttg acttcccaaa 180
gaacatcagc gtggtagctg tttgccctaa aggaatgggt ccatctgtga gaaggc 236
<210>128
<211>174
<212>DNA
<213>人工序列
<220>
<223>
<400>128
acacggtggg agacgcttct ctatgggata gtggtgtgga ctacacgagt tgggttaaag 60
gaaagacttt cagagttggt gacactctag agttcaagta tggttctaca cactcagtga 120
acgaagtgga caaagcaggt tacgataact gtggaggcac accgatcgat actc 174
<210>129
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>129
ggcaaaacta tatcaaactc tctttctaat catctcaatc tcctacttct tctcaccgga 60
gctcatcaca gcctcaccgg ctggagcttc ccaaaaagcc gtaaacttca tccaatcctc 120
ttgcaaaacc acaacatacc cagccgtgtg cttccgttca ctctccgcct acgcaaacgc 180
catccagaca agccctcaac gcttagccga gaccgcacta gccgttaccc taag 234
<210>130
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>130
ctgagatcac aaaggatata caaaactata gccaagggag ttgcagtttt gaacagcagt 60
gagggtgtta aggggactat cttcttcacc caggaaggag atggtgccac aactgcgact 120
ggaactgttt ctggtcttaa acctggtccc catggtttcc atgtccatgc tcttggtgac 180
accaccaacg gttgcatgtc taccggtcca catttcaacc ctgatggtaa aac 233
<210>131
<211>237
<212>DNA
<213>人工序列
<220>
<223>
<400>131
accagcccat agatttaaac caaagggttt tcttaaaact tgttcatctt atgcatcttc 60
tttaccaata catttaaagt ttgaggtttg taaggagttg accccatctt ggagtatcct 120
catcaaaaat gtaggtaaga ggtgaaacta ataccccact gcctccaacc gctctgagtt 180
gcttcactga ctcgtataag gccttctttg gcacacaaat cacaatggca tagtagt 237
<210>132
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>132
cattttctct caaatctgcg agcgatcgaa gaagaagatg acgagagaca ttgaagatga 60
gattcgagac gagaagaatc ctcgccccct cgacgaagac gatatcgctc tcctcaagac 120
ttacgggtta gggccttact ctgcgccgat aaagaaagtt gagaaagaga tcaaggagct 180
tgccaaaaag atcaacgatc tctgtggtat caaggagtct gatactggtt ta 232
<210>133
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>133
actcctgata gcaagcagcc atagctgctt ctctctcttg ccgctttctg aacgaatgcg 60
cacgaaacgc tgcctggatt ctcgcagctg cttgagctac gtttctcaat gcagcccgtg 120
agtgtggatc gtcattaccg ggaggacttc ttcctgagat ggaattcacg atcatctctg 180
cttgcatttg agctgagcct agagagtgtt ccgtttcctc gagtgtgagc gacg 234
<210>134
<211>176
<212>DNA
<213>人工序列
<220>
<223>
<400>134
agatcctaag tttagtacga aaaataanaa agcaaaccag taatcgaaaa tgaagcaatt 60
ctaatggata gagaaaaggg aatctctgca gctgcagcta tctagtgtag aggccgttgc 120
agtatccgtt gntgtgtctt acatcagcca agccattgga agctttcttt gagttt 176
<210>135
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>135
gtccctggac atgggacaat cccactgagt ccttacttct tctggcctag aaaagatgct 60
tgggaagagc tcaagactac tctcgaagct aagccatgga tctctcagaa gaagatgatt 120
attctcctta atcaagctac tgacatcatc aatctctggc aacaaagtgg cggcaattta 180
acttcccaat aaatcgtttt tttttctgca attcaagaat tcagaacctt tttttg 236
<210>136
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>136
accaggcggc actcagtttc gaaggcngaa ctgcacttcc tttgacggta atccgggact 60
ttctggccct tctcttgacg aaatttgcag aaatattcac atgtcaacac cgcatgaaac 120
gttggagtca gaggaagaag aagaggtgct gagttggata gcagttgtaa taggcgtcat 180
acctggtttt gcctttggat gggtgattgg ttacatactt ttttcctaca aacc 234
<210>137
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>137
acactgaacc aaaccgttta gtgttacaaa cggtgtcagc tttgtcttac cattacctaa 60
accttgactc tcacgcaaaa ccgcttcaga cctaatttta tcaaagagtt tcccgagttc 120
attatcgaac gttttgggat ctgtttcggt tacgttagcg acgttgtagt atatgagacc 180
agcacccgtg tcgagcttcc ctatgaaatt ctcttggctg taacgcaaga agcaga 236
<210>138
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>138
ctcctactaa aaattaaata ctctccagag atagatataa cgatggctgt ggggatcctt 60
gaggttaacc tgatcagtgg caaagctctc aaacgatctg actttcttgg taagatagat 120
ccttatgttg agatcaaata caaaggacaa actcgcaaaa gcagcgttgt taaagatgga 180
ggtagaaacc cgacatggaa tgagaaactg aaatggagag tagagtttcc tggc 234
<210>139
<211>175
<212>DNA
<213>人工序列
<220>
<223>
<400>139
gatgacactt ctacagaacg ggcacaccgg tggtgtcacg gttgaggtcg ttgggttagg 60
tttgttgtga cagcacagtg caagtgtgca ttgcgcacac atttggcgac cacagtcttt 120
gacttcaata gtgcatactt gctcaaagca aatgcaacag agttccgagt cactc 175
<210>140
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>140
atagctggtc caatcaaatc acggtcgaaa gtcaagttca taccgcacat tgggaaaagt 60
gttccctttg ggatggtcat cacagcatcc acatacctag cgttcctctc cttaggcttg 120
acgagctggg taggtgcgtc gtagtcaggg atgttgagcc agagaccatg ggaaacagca 180
gtggaaacac cttcacgaag actgaaaggg tatccacgga cgaagtcagc ac 232
<210>141
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>141
ttaatacatg caagaaaact tcatccgtaa aaagagtcat tttaacatcg tccacggctg 60
cggttcttgt tcgtcctttg gagccaaacg acgtggtgga cgagactttc ttctctgatc 120
caagtgtctg caccgaatta aagttatggt atccactctc caaaactttg gcagagaatg 180
cagcttggca atttaccaaa gacaatggaa tggacatggt cgtgataatt cca 234
<210>142
<211>238
<212>DNA
<213>人工序列
<220>
<223>
<400>142
cagctattcc tatggcaagt ccaatgtcag actcatgcaa tgcaggagca tcatttgttc 60
catctccagt cactgcaact acctctccaa ttttcctcaa attgttgact agtgtatgtt 120
tgtccaatgg caaagaccga gccatcacct ggattttggg taaaatggct ctcatttcat 180
gtgggggcat attacggaat gttgaacctt ctatagctac acctcctgct gtaagaat 238
<210>143
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>143
atggaagact tccttacgag aaactaaaac ctgaccccgt tctccgtaac cttcttctct 60
ctatgcctca ccgtaagatc atatttacca atgcagacaa agctcatgca acacgaacac 120
ttaaccgttt gggtctagag gactgcttcg aaggaatcat ttgcttcgaa acactaaacc 180
cttattctga tactaacact caagtcctct gtaaaccctc tgttcaagcc tttga 235
<210>144
<211>231
<212>DNA
<213>人工序列
<220>
<223>
<400>144
gccaaaaggc cgaggatcca taccttcatc gccacgagtg acattcactt ggagtataag 60
ctgaagaaga gtaaagacga ggtcatcgag atcgctagga atatggttaa gttcgcgagg 120
agcttggggt gtgaggacgt tgagtttagt cctgaagatg caggaagatc ggagagagag 180
tttttgtatg agattcttgg ggaagcgata aaagctggag cgacaacgct g 231
<210>145
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>145
gctttacatc cttgcaagtg acaacaaaga tacaagcagt tcagctctgg gatctatcca 60
agtcaatgct tctttcgttg aagagcttct agcctgtctc ctatcttgtg agccggggtt 120
atcatgcaac ttggtcaaag attacatttc accgacgaac acttgcccag gcaactatgc 180
tggcgtcatg agtggagaac cttcctccaa cccttacctt ggttccgtca gtgac 235
<210>146
<211>197
<212>DNA
<213>人工序列
<220>
<223>
<400>146
gatgtcgaga ttcagacacg agtcttgtgt gtttgcatct cggcttgaag ggataagacc 60
ccctcccctg ccggttccga tcttagccgg tatgatgtag tattggacat gggactgaac 120
tggatgtcct gatatgtcta ggactgcttt gcttggtaga gcattggcgg cagaagctgc 180
ggccacctgc ccgggcg 197
<210>147
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>147
cctcaaatac cgatccattt cccggcgata atcgacgatc tagatccgat ggcgaaggaa 60
ccagttcgcg ttctcgttac cggtgctgca ggacaaattg gatacgctct tgtccctatg 120
attgcgagag gtatcatgct tggtgctgac cagcccgtga tcctccacat gttggatatt 180
cctcctgctg ctgaagcact caacggtgtc aagatggagt tgatcgatgc tgcttt 236
<210>148
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>148
aaagaggcgt taacagcatc tttgatctgt tgagactcat cttctgattt gaagagtgag 60
gttatgaact ctatagaacc aagtgatgct tttcgtaatc tgctcgcttc tgctactgac 120
tcaacgccgt ttacagtagc agtaaggcct ctaccaggaa aatattcgaa gctttcaaca 180
aaaatagaag ggaggtcttt tcccacactg tggtctacca cagctcttcc aatag 235
<210>149
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>149
acggcaaaac tccggcccca cacctattcc tacggctagg atctttattc ctcttctcaa 60
tctcttgttc aatccgaccg atctccgccg agaatccata aaacgcatca acaatctcag 120
catctccggt ccaaatggac ggttgctgtc tctccccgat atactcttcg tccggcgaat 180
gcgtcgataa agtatcaacc accgccataa acttcgtcgt ttgcagcaaa ctcggc 236
<210>150
<211>231
<212>DNA
<213>人工序列
<220>
<223>
<400>150
actgggcgtg cagggaaaga aggctctgcc attctcatgc acagcagcag ccagaagaga 60
accgtgaggt ctctcgagcg tgacgtagga tgcagattcg agtttattag cccaccaacc 120
gttggagact tgttggaagc gtcagcagac catgtggtgg ccactctaaa cggtgttcac 180
cctgagtcta ttaagttttt ctcagcaact gctcagaaat tatttgagga g 231
<210>151
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>151
tttagagtaa aaggaaacac agtgcatagc aaaaaaacat aaagatcttt tagtgtttca 60
tcatgtcacc aagacaaacc atcttttttg tcaactttat tattattatt cacttctaca 120
aatccaacaa gaaaatgggg gaaaaaagat tagcaagcag cccgccatta aaaccaagca 180
agagggaagt gagtagaaat aaactactac ttgggatggc tcatttatca aggc 234
<210>152
<211>230
<212>DNA
<213>人工序列
<220>
<223>
<400>152
tttttgagag cagtagaaac gagatggaag gattgatcaa aggtttagcc gatgttgctt 60
tcggaggagg agacgagcgt cgcggaggag atgatgaaag ggacgagaga cccagatcca 120
cttgggctaa tgtggtttcc ggtgaggacg atgatcagaa ccgcgcggga ggatcggtgc 180
catcgcctca tggtcgacgc caaaatccag aggagaatca atgggagaag 230
<210>153
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>153
actttcccaa gctgtccgac aaagaagaaa gtcgagagat ctttggaagg acagatcacg 60
gagattgtct ataagggaag ccataaccat cctaaacctc aatccaccag aagatcatct 120
tcttcttctt caacatttca ctcggctgtt ttcaatgcca gccttgataa tagtttcagt 180
cactctgatt cccttgcaat ccaacaagat gataacacta cttctggttc tgtcg 235
<210>154
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>154
ctcaccttac ccattgagag aactcagaga agatgctgcg agttgcaggg aggaggcttt 60
tgtctcttca gcagagatct tccaccgcga cctccttcgt cctttccaga gatcacacca 120
tctccaaaga aggcggcgag gactccacca gatctgctgc tccttctgca gatctttcat 180
gtttcagttc ttaccaccgg agtcttttaa gaggtttctc ttctcaagtc atta 234
<210>155
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>155
acacagaccg gaactgctga agggttcgct aaggctttag gagaagaagc tagagcgaga 60
tacgagaaga ccagattcaa gatcgttgat ttggatgatt acgcggctga tgatgatgag 120
tatgaggaga agttgaagaa ggaggatgtt gctttcttct tcttagccac atatggagac 180
ggtgagccta cggacaatgc agcgagattc tacaaatggt tcaccgaggg ag 232
<210>156
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>156
tttcagcaag ctctcgcttt atctctctta gtcgatcgtt tgatgaagat caggtgcgac 60
gtctgcgata aagaagaagc gtcggtgttt tgcaccgccg acgaagcgtc tttgtgcggc 120
ggctgcgacc accaagtcca ccacgctaac aagcttgcct ccaaacatct ccgtttctct 180
ctcctatacc catcttcctc caacaactcc tcccctgtct gcgacatctg tcagg 235
<210>157
<211>294
<212>DNA
<213>人工序列
<220>
<223>
<400>157
acaccccagt ggaaaaccaa tcaccctcag gagtaggagt cacaagagac tttatagcat 60
caacaatgga aacagctgta gaagcagcag acgaccgacc ccatttctga attagtaagc 120
cacctctctt ctgcacactc tcagtgaatc cctcttctaa ccatttgtgg tctgtaataa 180
cctccttcac cggctggcca ttaattcttg catttaagaa gtctggcacc tgagtcgtgg 240
agtgatttcc ccatatggtc atattagaca ctttgtcgta gaaaacgcct gctt 294
<210>158
<211>177
<212>DNA
<213>人工序列
<220>
<223>
<400>158
acttgttgtc ccatggtggc tgatcatacc tcgtccgatg gcactacgac tttggtcgtg 60
ttgtctggac tgggtgagca gatcaccctg gctccccaga tgtctgttga aattgatcat 120
gatctcagaa gaggtgagat ccgtcttggt agccttggag gtatgttttc aaagact 177
<210>159
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>159
accagcttgc tggtgtggta gcagttgaag tgactggtgg acctgacatc agttttgttc 60
ctggcagaaa agattcaaat gcatgcacgg acgaaggacg acttcctgat gctaatcaag 120
gttccaaaca tctgaaagat gtcttccacc ggatgggact gtctgacaaa gatattgtgg 180
cactgtcagg ggcacatact ctgggaatgg ctcatccaga gagatcaggt tt 232
<210>160
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>160
actaaagctg cggagaaacc caaggcatca ccaccgccac caccgccgtc taaacagtca 60
gcgaaagaac cgcagcttcc tcctaaggat agggaaagac gggttcctat gacaagactc 120
cgcaaacgtg tggcaactag gctgaaggac tctcaaaaca cttttgcgtt gcttacgaca 180
ttcaatgaag ttgatatgac caatctgatg aagctccgat ctcaatacaa gg 232
<210>161
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>161
gttttctgat cacttgttgt tcggttgttt agcgtgtctg agccatggct tcaaagagga 60
ttctgaaaga gctcaaggat ttgcagaagg atcctccttc taactgcagc gctggtcccg 120
tggctgagga catgttccat tggcaagcaa ctatcatggg acctcctgac agtccctacg 180
caggaggagt gtttttggtt tccattcact ttcctccgga ttatcccttc aagc 234
<210>162
<211>176
<212>DNA
<213>人工序列
<220>
<223>
<400>162
acgcagttca atagagaact ttcttgcgca ccaagttctt cgacaaagat ccgatgtgca 60
taggctccag caactccatg cattgcatca tagcagaatg aaaactttgg agatgaaagc 120
aacttccgga tggattcaaa gtcgaagatt gacttcatta gtttaacgta gtcatc 176
<210>163
<211>177
<212>DNA
<213>人工序列
<220>
<223>
<400>163
acatcctctg tccatccagc attatagaag caatcggaag tgacagaaat accacccagt 60
cctctatggt tgcttataac aacattccaa ccggattttg cagtgttata ggcaaggtgc 120
tttagatatg cagaagaaga atcactagtt agccctggaa taacaacagc aatagga 177
<210>164
<211>231
<212>DNA
<213>人工序列
<220>
<223>
<400>164
gttccacaac tttggcatcg acgcacacgc aaacacactc gcttcaagcc tcgtctctcg 60
cgatccagac ctctcaaaac gcatgatctt ccacacaacg gacgtgctaa acgctaccga 120
agggctagac caatacgacg tagttttctt ggcggctctc gttgggatgg acaaagaggc 180
aaaggtcaaa gccatcgagc acttggagaa gcacatggct cctggagcta t 231
<210>165
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>165
gaaaaaaagg aagagagaga agagaagaga gagagaaggg gattcatcat caacccatct 60
tccctccctt cctgtggatc tcaaaattcc tgggttttct ctggatttga tccgatcaag 120
atttgaagac aaaggaagaa tctttttctt tttagaagtt tctaatggcc acggtgggcg 180
tggagcctag tgccgcggtt agagaatcta atggaaacgt tactgctgat gtt 233
<210>166
<211>179
<212>DNA
<213>人工序列
<220>
<223>
<400>166
acagactctc actcgctttc tccaacgatt atcctttcaa acctcccaaa attaagtttg 60
agacttgctg cttccacccc aacgtggatc tctatggcaa tatttgcttg gacattcttc 120
aggataaatg gtcatctgct tatgatgtga ggacgatatt actctcaatt caaagcctt 179
<210>167
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>167
acccatcttt cgtttgaata gtgtgttcgg tgcaggagta accggccgga tgaatgaggt 60
cagcgcagag agagttgacc ggagatccaa ggaggatatg agattgtgtc acggcggaag 120
aaatcgtaaa agcggtcaac acggcgacaa gtaaccactt catcgtcgcc ggagacgacg 180
ggctgttctt atcctaaaac gtaagattac aaccttaccc tcttcgttta ta 232
<210>168
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>168
ggagtagtga atctatcaaa ccggcaactt cagcctctcg actctctctc tgatggcgtc 60
ttcaagcccc caacattgcc acatcatcga ggtcaatcga ggtaaatccg ctgaagaaag 120
cacaacaatt ctggcaagca aagcctgtgg agaagctccc cgcggcttct cagatctcaa 180
caacgcttcc ggcgacgccc aagaacgcaa tgcctccatg cgcaagctct gc 232
<210>169
<211>175
<212>DNA
<213>人工序列
<220>
<223>
<400>169
gcattttctc cgatgacgct gcgccgccgc cgcagcagga gcagtccttc tctgctgacc 60
tcaaactctt cgtcggtaac cttcccttta acgtcgacag tgctcagctc gctcagctct 120
tcgagagcgc cggaaatgtc gagatggttg aggtgatcta tgacaaagag acagg 175
<210>170
<211>237
<212>DNA
<213>人工序列
<220>
<223>
<400>170
acactttaca caaactaaaa tacactttta cacaaatttt attaggattg agattcctta 60
ccatgtatca ataaaattct aagcaatatg aacaaggcgt gaggactgaa cgaaaccatc 120
gacccttttc ttgatctcat caggatttgc gcccaccagt ttgtccatcg tattaccatc 180
cttcaagaac ataaaagtag gcatagcctt tacctttagc cgtctcgcca cttcctt 237
<210>171
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>171
actaacttag ccaccatctc cacaggcaca cctttaagct ctgcatgagg cttgaagttg 60
aaaagtggtg gaaagaaacg tccattaggc aaccttgcat tcggatctct aagctcgtca 120
aagaacgggt ggatcaatgc atcaagagca ccgcaacgga gactaggtga gtattgaaga 180
agccttgaga ccaaatcaac agcttctgga ggcatcctct tgtgaaaaat ct 232
<210>172
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>172
cttctaatca cattggtctt gatggtcttg acgggattgt acttgtagaa gataggagac 60
gcaggacaag cgagatggta gatcctatca acctcgatca acaaaggctc cgtaacatcg 120
tgacgaatga gctcaaacct tgggtgaccg atccacttct tgaggttttc ttttgatcca 180
gtgaagtagt tatcagcaac aatcacctca ttcttctcat tttccataag cttatc 236
<210>173
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>173
acgtctagca atcgaataca tagtcatccc aggaggtgaa ccggtggagc ttcttccaag 60
acggtcagac ataatagtcc ggcagctaga gctggtagag agttatcaac tcgcggtgga 120
gaatctcggc actcacctta accctcgtct tcagatactt cctcgaagat ccaccaaaaa 180
aaatgccgtc tccaaagaaa gcaggagatg atagcatggg aaatacagtg ac 232
<210>174
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>174
cgtcaatttt gcaaggaaac gtccgagaaa attaaccgcg cagagagtta ttttgaaaaa 60
aaagttatcc aagaaccgtt gaagctgtcg ttgttgttca tggagcttcc tcaacctcgt 120
cccttcaaag ctcaagggag aaaaccaaca catgattttt tatcgctctg cagtcatcca 180
actctccagc cagatccaaa gcctacacca ccaccttctt ctcaaggtag cca 233
<210>175
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>175
tttttcgtaa atacagcgag tcgcatgctc cggccgccat ggcggccgcg ggaattcgat 60
tagcgtggtc gcggccgagg taccggagaa tctctccaat gagttctcct ttaccggaac 120
caacataccg cccacaaata tcaccgtttc taatgaacaa gaacgtgggc acttcgataa 180
cattcatgtc ctttagaaac tccatacagc tatcgttctc gtccccgttc attct 235
<210>176
<211>228
<212>DNA
<213>人工序列
<220>
<223>
<400>176
acaggttact ccagcgggca acacgagagt cccgtgagtg tcgagacagt tgctgataag 60
ttgactactg aggacgaaaa tgtcaaagaa actgcatcgc ctgtgacggc gaaactgcct 120
ttctctggcg atgggagtga ggtggatgag acagaacaag gggaagacaa atttgtggcg 180
agtagagatc atctggagga gaagctgaca cctgaagaag aagacaaa 228
<210>177
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>177
acaagctcag gagtaacatc aagagcctta acctctccgg gatgggatgc agcgctagtc 60
ttatctctgt tgatgtagca cgcgacttgt tacaagttca tcctaactcc aacgctgtga 120
tagttagcac tgagatcatt actcctaatt actaccaggg caaagagaga gctatgttgc 180
tcccaaactg ccttttccgc atgggagcag cggctattct tctctctaac cgcaga 236
<210>178
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>178
tagttaatgc attggcctaa gttgctggcg actgcgccgc aagagattgc tgcctcggac 60
gccattggag cggacactaa catgcacaca atcaccaacg tcgacaactt taagagcccc 120
tccataaatc ttaaagtttt atctcttatg cttaagtaaa gtaatggggt tttgggatgc 180
ttgttggaga tgttccttgg ggagatgtgt tcgtcttgta agagggctaa aggat 235
<210>179
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>179
acttgtgtgt gaacaaagag tcaaatgcta aaaaccaaag ttacatcttt cctcctcaca 60
caagtcttta acatactaat caatcctctc actctacgtc ttctggagct ccaccaggca 120
ccaaaatggc gatgttgttg ccgttgagga gaatctgatc aagctttgtg acccgacttc 180
cttcagctgt aatctcatac tcggtgacat cctcaaggac catgttgacg taaa 234
<210>180
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>180
caaggctcca ctgggttatt ctggaccgtt tgagctgaga ttgcttaacg gccaagttgc 60
tgattctccc actaacatct tggaggagat tacatggttc aaagaccaag aagtttccag 120
gatgaaggaa ctgaacccac ttgaggcgtt aaagaaagct gtagagaatg ctcctcccac 180
tagggatttt gtaggtgcgc ttaggagtgc tcatcaaaga actggcttcc ctg 233
<210>181
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>181
caggcaaccg cacttaaacg gtgcgtatta cgggccatcc attcctccgc cgacaaaaac 60
ctctcacagc cacggcagac gcggcggtgg atgctgctgt ttaggtgact gtctcggctg 120
ttgcggctgc tgcatcttaa gcgtcatctt caacatcctc ataaccctag tcatcgtcat 180
tggcatagcc gctctgatca tctggctaat tttccgacca aacgccatca agttc 235
<210>182
<211>239
<212>DNA
<213>人工序列
<220>
<223>
<400>182
acgtgtcatc atcgtttcca tcatcggtaa agtcacctgt gttgctgact ccccagttaa 60
tcgagtgctg atttgttgag gcggtaggct caatgttatc agcttgataa ttgattttac 120
ccagagagtt ttttatattc acactttcgc caccacagtt aatatgtaat gatttttgat 180
agcccagaca cttgattgga ccagcgcatg gaagcatccc acttaaatta tctttccag 239
<210>183
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>183
acattaaaag acaaccataa caaataaatc tccaaaagaa ttaatattaa aacaaaacta 60
ggacgacgta acgggagtga tcctaggaac tgcaacagga aactcatgaa tctcatcact 120
ccatggatta gaattttcaa ccggatcaac cgttctcaaa gctttccaac ccacaagcat 180
aacccctaat ttggggacac gagtatcggt tttgaaatcg tatttgacaa ccttg 235
<210>184
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>184
gtgtatgaga agaaagttct cgtatatcat agttatattt tgacaaataa ttaaaaaggt 60
aaaacatgga gaaaggtgga gttgagcatg agataactga gctgagattg ggtcttccgg 120
ggagagatgt gacggagaag atgatgaaga agagaggctt cacggagatg atcatgacgt 180
cttctggaag taacagtgat gaaagtggtg tcgtttcagg tggtgacgtg gag 233
<210>185
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>185
cacacattaa acttcgtttg cgagcatctc gaatcgagat gggttgttca actcccttct 60
gctactcaat caacccatct ccatcaaagc tcgatttcac caaaacccac gcgttcagcc 120
ccgtcgccaa acagttctat ctcgacttac cgtcgttcgc cggcaaatcg ggagggggat 180
tatcggggtt aagaaagcgt cgagctttgg tcggagtaaa ggcggcgact ttgt 234
<210>186
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>186
acagctcttc tgattcacag tcgcttctgg gaaaccctgc gtctagctct tcttctctga 60
atcatgaatc tacaaatctg ccaaagcctg tgattcaaga ggaaccggga gtctcggcca 120
cagctctccc aaagaatcgc tgttacagca ctagtaataa ggaagttaag ccgaatttac 180
caacggtgac agagccgaat aatgaagaac aaagttgtga aaagccacat ag 232
<210>187
<211>239
<212>DNA
<213>人工序列
<220>
<223>
<400>187
acgagtccat gatgatgatg ttggagcctt tgttgattct cttgaggtaa gatatcttta 60
atgcagctat ttctgcttca acacgttttg agttcctcac gattcctttt actttgttcg 120
gtagctcttc caacggaatg gcgatcatac tgttctttgc atttgaaggg agacgaggaa 180
tcccagcttt ttctttttct ttctctgatc ttatatccac catcaagtag ttcttggaa 239
<210>188
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>188
cggtggccgt aaacgcccaa cacgacaaag ccgtgactct tcctgggatc gccatcgttt 60
ggggactcac cgtcatggtt cttgtttact ctctcggtca catctctggc gctcatttca 120
atccggcggt cacgattgcg ttcgcatctt gcggccgttt ccctctcaaa caggttccgg 180
cttatgtgat atcacaagtg atcggatcaa cgcttgcggc ggcaactttg cggctt 236
<210>189
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>189
ccaaatggga accgctctaa tagagcaacc ggttccggtt attggaaagc taccggtatt 60
gataaacggg ttgtgacctc tagaggaaat caaatcgttg gtttgaagaa aacacttgtg 120
ttctacaaag gcaaaccacc tcatggctca agaaccgatt ggatcatgca tgaatatcgt 180
ctctcttctt ctcctccgag ttctatgggc cctactcaga actgggttct ttgtcg 236
<210>190
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>190
actaacgttc catctttttc tcctacgtag aagataaatt ttgaaagaaa aaggccactc 60
ctatgttcac tgaaagcttc atgttctaga ctgctccatc aacctcccag atatagcagc 120
cgcaagagca gccgtaaact ttgaatcttt cgttaaggaa gacgccattt gctgaaccaa 180
aacctcttgc atagtttccc tttcgttctt ctccaaagct aaactatgac caccat 236
<210>191
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>191
ttcatgagtt caagcagtgt tttgacccca acaccatttc ttggacagac taaaggctcc 60
accttcaacc ctcttcgtga tgctgtctct ctcggatctc ccaagtacac tatgggaaat 120
gatctttggt atggaccaga cagagttaag tacctaggac cgttttccgt ccaaaccccg 180
tcttacctca ccggagagtt tcccggcgac tatggttggg acaccgccgg tttat 235
192>DN965527 late embryogenis abundant protein
<210>192
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>192
gtagcagaca gacaaaaaca gggttcaccg caaaggatca ttaccctatt ccccaagaca 60
ttaaaaatca acaacgaata attaacagaa aaaaaagagc ttcttttcag ttaactcatc 120
actgctcgtt gttcaagaga gctgctcgta gctcagctgg gtcgatctct tttgaaccgg 180
tttccggtct gtaataaccg gttttgggat ctggaatcca agctatcttc tggg 234
<210>193
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>193
gtacaaaccg aatgttgtcg tctacaacac tctcatcgat gcttattgta aaggcggacg 60
tgtgagtgat gcactccagg tgttcgacgt aatgaagagg aaaggggttg cgccgagtgt 120
tgttacgtat aatgctgtga taaggggtct ttgtaagtcg gggaggtgga aggaagctga 180
tgggttgctt agcgatatgg tggagaagag aattgatccc aatgtggtta ctt 233
<210>194
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>194
ttctccactc ccacgtgacc tgcgtcggtt ttcactgcgt ccccttcaac gtgaggaggt 60
tcttcggtgg acttgtagat ccccgcgctg cttatgaaca agaactgctt aacgccagag 120
cttttagccc aatccaccac tggcctgaca gtatccaagc ccttgccatt gttgtcaaga 180
acaacgtcga atgtttcacc tccgaccaca gtcgcaacat cagccgggtt tccc 234
<210>195
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>195
cattgatcga gacatctgca agctcctaac ttgtttcaac tctttggtcc gatcatggcc 60
gtcgctgaga atgctggtgt gaaagtggac tcttctggtc acaatttaga caacaacaac 120
actgcttctt cagctaccga gaccaatccc tcctgtcctg atgatcagag ccctaaatcc 180
gattcctctc tcgactcctc atcggatcct ccgactccag actccgacga tcgga 235
<210>196
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>196
acttcctagg tttgtagata gctagccaaa ttatgcaagg aaggaagtat gtcgttggag 60
caaacgcgaa tccaccaaag aacgctaaaa gcccaccaaa gaaaggaaac gtcataccta 120
gaaacattgt tacagcaacg tagaaattgc gaacacagaa ccggagaatc gtggtaggtc 180
tgaaattaag cttcttgacc aacaaagttt ccatcatgtc aaaaactggc att 233
<210>197
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>197
acagatgaga ggttcagcaa gctctttaat cagacaggat tcaccatcgc tgtcgtgaag 60
aaggctcttg aagtttacca aggcttcaaa gacgtggatg tgttggttga tgttggagga 120
ggagttggca acactcttgg tgttgttact tctaagtatc ccaatattaa gggtatcaac 180
tttgatctga cttgtgcctt ggcacaagca ccttcttacc ctggggtgga acat 234
<210>198
<211>231
<212>DNA
<213>人工序列
<220>
<223>
<400>198
acatccgaac atcaattcag tcttcctgcc gctaaacttt acaaggagaa agtggaggac 60
cgcaaagaca gtcgttccca gtaaacgctc tggccggaaa ctttgttgtc ggaagctttc 120
cacagaggtg attctgactc acgttcagtg tctgcagtcc agcaactgta gccggcacct 180
tcccaaacac caagtttctc gacagatcca gcgttttcag cgtcttcgca a 231
<210>199
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>199
caagagtgtg atgcaccgtg tggtgactca gaaagaagga aacagaatgt caattgcatc 60
tttctacaac ccgggaagcg atgctgagat ctctccagct tcatcgcttg cctgtaaaga 120
aaccgagtac ccgagttttg tttttgatga ctacatgaag ctctatgctg gggtcaagtt 180
tcagcctaag gagccacgct tcgaggcaat gaagaatgct aatgcagtta cag 233
<210>200
<211>231
<212>DNA
<213>人工序列
<220>
<223>
<400>200
actgctcaac gtggaaagga aaccaaccgc tcgaatggcg taggaagtat gggtggacag 60
ctttttgcgg tccggtcgga ccgcgtggtc gtgattcttg cggaagatgc ttaagagtaa 120
cgaatactgc gaccggggca caagctactg tgagaatcgt ggatcaatgc agtaacggcg 180
gtttggactt agacgatggc gttttcaaac agcttgacgc caacggtcgg g 231
<210>201
<211>175
<212>DNA
<213>人工序列
<220>
<223>
<400>201
acatgcttct cctccatggt cagcaacgcc ctttggcaaa aagggcagtc tccgagcttg 60
ttgggtgtgg tgatggaagc tttgacgcag atttcgagag gagcagccgc catagccact 120
gttcctaacc ggacggttct tctcggtttg gtcaaaccga atctcttgat aagcc 175
<210>202
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>202
actcctcacc ctccacgaga aaaacctcga ctttgagttc gttcatgtcg acctcatggg 60
aggtgagcac aagaaagagg ctttcctagc ccgcaaccct tttggtcaag ttccagcctt 120
tgaagatgga gacctcaagc tctttgaatc aagagccatt actcaataca tagctcaccg 180
atacgaaggc caaggaacca accttctccc agctgactcc aagaacatag cc 232
<210>203
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>203
aatgctggtc ctgaaaagga agtgaagttt gttattgtcg gagagaaagc gaaggctata 60
atgttccgtg actcaaagaa cgacatctcc cttactgtaa cagagctgaa taagaatcca 120
ctcaattatg cccaggtctc agttctagct gatgacatct tgaagaacgt tgaatttgat 180
gctttgcgca ttgtctacaa caagttccat tcggttgtcg cgtttttacc aactgt 236
<210>204
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>204
ccgaagatgg cggatccatc gctgcaaggg cgttatccaa tgcgtggtct gtatcaagcg 60
cttgcggttg cagcaatgtg tttacaggaa caagcagcaa caagaccgtt gataggcgac 120
gtggtgacag ctctaactta cttagcttct caaacttttg atcccaacgc gccaaacggc 180
caaaacagta gaagcagtgg cggtggcggg ccgccgttca tcaggacgag gga 233
<210>205
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>205
aagggtagat acccagacat catgaggcag attgtgggta gtaggcttcc caacttcacc 60
gaggaagaag cagaactcgt tgctggttca tatgattttc ttggtctaaa ctattacgtc 120
acccaatacg cccagccaaa acctaaccca tatccttcag agacacacac tgccatgatg 180
gacgctggcg taaagctcac atatgataat tcacgtggtg aatttcttgg tcca 234
<210>206
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>206
accgtttggt gttggacgta gaagctgtcc tgggattata ttggccttgc ccattttagg 60
aatcaccatt ggtaggttgg ttcagaactt cgagctcctt cctcctcccg gacagtctaa 120
aatcgatacc tctgagaaaa gtggacagtt cagcttgcag atccttaacc aatccacaat 180
agtaatgaaa ccaagggcct tctgagaaaa gtaatttctg ccaaatgact tcttg 235
<210>207
<211>173
<212>DNA
<213>人工序列
<220>
<223>
<400>207
acggaagagc caggaggaga ggaatggagg agatgaggaa gggaacggtg agcgttgggt 60
tgaagtgata tcgtgggagc ctagagctgt tgtttaccac aatttcttga ccaatgaaga 120
atgtgagcac ctgatcagtc tcgctaaacc caacatggtt acgtcaactg tgg 173
<210>208
<211>237
<212>DNA
<213>人工序列
<220>
<223>
<400>208
actccagctt cttcttcttg ctcttcttgt tcgtgttttt gtttgtctcc tcgacctcta 60
ccttcataat actcctcctc cctccgatca tttccagcaa cacgatccca tagctgtaaa 120
cgtcagactt ttcagagata ccgtgttcaa gaagccactc tggggctagg tatccacacg 180
tgccgcgaat ctccgtcaca actcggctct cgtctctagc gatcagcttt gagagtc 237
<210>209
<211>237
<212>DNA
<213>人工序列
<220>
<223>
<400>209
acttcgattt tattcagagc agctagtgtg gtatcatcat attgattatt attattattg 60
gggttttgcg gttctccctc cagcctcttg ataatcacac tttcattcac ggacagaaaa 120
taacattgta gttggttccg ctagggcaag tgaaaaggct ggtgggatca tccttagggt 180
aactataggc atctgggcaa cgttgcttga aaaacatcga gagtggggtc ggtccac 237
<210>210
<211>235
<212>DNA
<213>人工序列
<220>
<223>
<400>210
aacactgatg agtccattcg tgctttcgct gaggcatcga tgaacactac ttatgagaaa 60
aagtggccac tttatcttag cacaaagaac accattctta agaaatacga tggcagattc 120
aaagacatct tccaggaagt gtatgaagcc agctggaaat caaagtatga agctgctgga 180
atatggtatg agcaccgtct cattgatgat atggtggctt acgctctgaa gagcg 235
<210>211
<211>292
<212>DNA
<213>人工序列
<220>
<223>
<400>211
ggaaaggtcc atggactgaa gacgaagatg ctattctcgt caacttcgtt tccatccacg 60
gtgatgctcg ctggagccac atagctcgtt cctcaggact taagagaact ggtaagagtt 120
gtagattaag atggcttaac tacttgcgtc cagatgttag acgaggcaac atcactctcg 180
aagaacaatt tatgatcctc aaactccatt ctctatgggg caataggtgg tcaaagattg 240
cacaatactt accgggaaga acagataatg aaataaagaa ttattggagg ac 292
<210>212
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>212
atggtggaac agggagtggt gaaaccactg tgtgatcttt tgatatgtcc cgatccaggg 60
ataatcaccg tgtgtctaga aggattggag aacattctga aagtcgggga agctgagaag 120
gtgactggaa ataccgggga cgtaaacttt tacgcacagt tgatcgacga tgctgaggga 180
ttagaaaaga ttgagaatct gcagagtcat gacaacagcg agatctatga ga 232
<210>213
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>213
catgcttgct tatcagggaa catcttctgt ggttaattat gattcttgtt tggctagttt 60
gatatccaag acaaatgtct ttgttgtaga aggctacttg tttgagcttc ctgatactat 120
aagaaccata acaaaagcct gtgaagaagc acacagaaac ggagcgcttg ttgctgttac 180
agcatcagat gtgtcttgca ttgagaggca ctatgatgac ttctgggaca ttgtgg 236
<210>214
<211>294
<212>DNA
<213>人工序列
<220>
<223>
<400>214
taatgtcaca gaaaaacatg cttaaaacaa acaggaacag tctaaaaatt gtgaaaatct 60
tttattttga taaatgcaac aatcccccag atgacttgtt tcccttttca attagctttt 120
gactctatta ccttcttggc gaaagaaggc aagcaaaacg cagctgaatg aatctgaaac 180
acagaagaaa ttagaacatg agttaaggat ttgaatatct agacgcatga aatggtttaa 240
tgagagtttt tgtgttgcct taacctcagc attgtaaaac ttcaagggtg catt 294
<210>215
<211>233
<212>DNA
<213>人工序列
<220>
<223>
<400>215
tattaatacc cgggactggg atattgagcc tctctcaact gtcctgaaca cagatgtgac 60
taacactgag tcgagctcca ctcctctatc ttcgttacaa aataagagcc caacaagacg 120
ccccaaaagc agatgggagc cgttagtgga atccaaacca tttgtgaagc cagcttcaac 180
tttcaccagt ggtgttaagt ttggaggttg gaatcaccca aatgtaaata aca 233
<210>216
<211>175
<212>DNA
<213>人工序列
<220>
<223>
<400>216
gagtcaggaa gagtctgcct gagtctgttg aatacatgga atggctctgg cactgaaatg 60
tggaacccag ggagctcctc aatccttcaa gtccttctct cgtttcaggc tcttgttctg 120
aatgagaagc cttacttcaa cgaagctggc tatgataagc agttgggccg agctg 175
<210>217
<211>237
<212>DNA
<213>人工序列
<220>
<223>
<400>217
tgcactgatc gcgccgagct cttcaggcag gagagctatt ttgcttactt gtttggagtc 60
agggagcctg atttctatgg agctattgac gttggaagtg gaaaatctat tctttttatt 120
ccaaggttgc ctgaggatta tgctgtctgg ttaggggaga taaagccatt gtcccacttt 180
aaggaaacat ataaggttga catggttttc tatgtggatg agattgtgca agtcttg 237
<210>218
<211>234
<212>DNA
<213>人工序列
<220>
<223>
<400>218
ttcatagata ttgttgaaac aaagcctgct aatggaatca gcatcattga aactgactgt 60
gaggttgatt ttgcacctcc ccttgattac aaagagcctg aacgtcctaa agcacctact 120
gctgcagcta aggctctagc aaaagctgag gaagttgtgc ctgaacctga gccaaagttt 180
aatcctttca cgggatctgg aagacggtta gatgggagac ctctttctta tgag 234
<210>219
<211>232
<212>DNA
<213>人工序列
<220>
<223>
<400>219
ggacaggtgg ccatagtcac ccaagtagaa actgtaagtg gcttcgaacc ggtcaccgtt 60
cttctccggg acgtgttgga tcaagacaca gagaccggcg gtgattccca cgcgcttttt 120
gagatcgccg gtgtagagtt tgttggtgaa gggcacgaga tcgccgaggc agagctctgg 180
tttcttgccg agttttagaa cagctgggct gtttctatct ccttcgttga gt 232
<210>220
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>220
tactttctcc tgactgggac tcggtaagct tagtgctgaa gtcccaaacc ttcttggcca 60
attctgaatc tttggcaagt gatattggtt tagcaatgtt gctgtccaag aagtattccc 120
ctgtaactcc tgtaacctga ggactcaatg ccacatagca agtcgttgcc gccccctgcg 180
gaacagtctt gagcacatat ttagccactg caccaacagc tccagccaaa tagctg 236
<210>221
<211>236
<212>DNA
<213>人工序列
<220>
<223>
<400>221
atgccattcg gaccggtcga aaccgctata ccgtatcttg tccgacgtgc ttatgagaac 60
cggggaatga tggccacggg agccactgac cgtcatctca tgaggatgga actaaagagg 120
agattactcg ccggaaatgc gtgaggagat gagtttaatt gtccttatgt atggagccat 180
taaattgaac tgggaaatgt agattcacaa atttgttcta tgtagattat aagaaa 236
<210>222
<211>24
<212>DNA
<213>人工序列
<220>
<223>
<400>222
agcggataac aatttcacac agga 24
<210>223
<211>24
<212>DNA
<213>人工序列
<220>
<223>
<400>223
cgccagggtt ttcccagtca cgac 24
Claims (7)
1、一种检测十字花科植物在胁迫条件下基因表达信息的cDNA芯片,由序列表中SEQID №:1-221 DNA序列的探针,以及具有下达GenBank登录号的探针序列组成:
CA991436,CA991442,CA991443,CA991447-CA991450,CA991452-CA991458,CA991460,CA991462,CA991463,
CA991465-CA991470,CA991473-CA991482,CA991484-CA991493,CA991495-CA991500,CA991502,CA991503,
CA991505,CA991508,CA991510-CA991522,CA991524,CA991525,CA991527,CA991528,CA991530,CA991532,
CA991534,CA991536-CA991540,CA991542,CA991544-CA991546,CA991548,CA991549,CA991551-CA991566,
CA991568-CA991572,CA991574-CA991577,CA991579,CA991581,CA991582,CA991585-CA991603,
CA991606-CA991610,CA991616-CA991654,CA991666-CA991668,CA991671,CA991673-CA991675,
CA991678-CA991681,CA991686-CA991693,CA991698-CA991700,CA991705-CA991707,CA991710,
CA991714,CA991715,CA991718-CA991720,CA991723,CA991725-CA991727,CA991729-CA991733,CA991735,
CA991737-CA991763,CA991770-CA991773,CA991776-CA991778,CA991781,CA991784-CA991790,CA991792,
CA991793,CA991795,CA991797-CA991804,CA991806-CA991808,CA991814,CA991817-CA991822,
CA991826-CA991832,CA991834,CA991835,CA991839-CA991842,CA991848-CA991852,CA991854,
CA991855,CA991857,CA991858-CA991860,CA991866,CA991869,CA991872-CA991875,CA991877,
CA991879-CA991882,CA991887,CA991892,CA991895-CA991898,CA991900,CA991901,
CA991903-CA991905,CA991907,CA991909,CA991911,CA991912,CA991915-CA991927,CA991930,CA991934,
CA991936-CA991944,CA991946-CA991954,CA991960,CA991961,CA991964,CA991966,CA991968-CA991973,
CA991976-CA991984,CA991987,CA991989,CA991991-CA991995,CA992000-CA992002,CA992005,CA992007,
CA992008,CA992010-CA992012,CA992015,CA992017-CA992025,CA992028,CA992032,CA992033,CA992037,
CA992041-CA992045,CA992053-CA992057,CA992061,CA992062,CA992065-CA992069,CA992072-CA992076,
CA992080,CA992083,CA992086,CA992088-CA992091,CA992093-CA992097,CA992101,CA992105,CA992114,
CA992118,CA992120-CA992122,CA992125,CA992127,CA992130,CA992132,CA992138-CA992146,
CA992148,CA992150,CA992155,CA992159,CA992161,CA992163,CA992169-CA992172,CA992175-CA992178,
CA992182-CA992192,CA992195,CA992198,CA992202,CA992203,CA992206-CA992208,CA992212,CA992214,
CA992215,CA992218,CA992219,CA992221,CA992223-CA992225,CA992227,CA992229,CA992232-CA992234,
CA992237,CA992241,CA992247-CA992249,CA992253,CA992254,CA992259-CA992267,CA992272,CA992273,
CA992277-CA992283,CA992287-CA992289,CA992292-CA992296,CA992299,CA992303-CA992305,CA992308,
CA992310,CA992315,CA992319,CA992324-CA992333,CA992336,CA992338-CA992350,CA992355,CA992356,
CA992359-CA992366,CA992373,CA992376,CA992378,CA992380,CA992385-CA992388,CA992391,
CA992392,CA992394,CA992396,CA992400,CA992401,CA992406-CA992418,CA992421-CA992424,CA992428,
CA992431,CA992432,CA992434,CA992436,CA992439-CA992442,CA992445-CA992448,CA992450,CA992451,
CA992458-CA992461,CA992464-CA992473,CA992476-CA992481,CA992484,CA992487,CA992488,CA992492,
CA992496,CA992498-CA992504,CA992507,CA992508,CA992510-CA992513,CA992516-CA992524,
CA992530-CA992538,CA992540,CA992542-CA992546,CA992553-CA992569,CA992573-CA992579,
CA992583-CA992585,DN191370,DN191373-DN191379,DN191385,DN191388-DN191391,DN191394-DN191397,
DN191399-DN191401,DN191403,DN191406-DN191413,DN191417,DN191419,DN191422,DN191424,DN191429,
DN191430,DN191433-DN191435,DN191438-DN191443,DN191445,DN191450,DN191453,DN191456,
DN191458-DN191462,DN191464,DN191467,DN191468,DN191471,DN191473,DN191474,DN191476,DN191479,
DN191483,DN191485-DN191488,DN191491,DN191492,DN191497,DN191498,DN191502,DN191503,DN191506,
DN191507,DN191510-DN191526,DN191530-DN191532,DN191534-DN191536,DN191538,DN191543,DN191544,
DN191546,DN191548-DN191554,DN191556,DN191559-DN191561,DN191563,DN191564,DN191570,DN191572,
DN191573,DN191575-DN191577,DN191579,DN191582-DN191586,DN191588-DN191590,DN191594,DN191597,
DN191599,DN191603-DN191607,DN191609-DN191618,DN191620-DN191626,DN191629,DN191631-DN191634,
DN191636-DN191638,DN191642-DN191644,DN191647,DN191649,DN191651,DN191653-DN191655,DN191659,
DN191662,DN191665,DN191666,DN191670,DN191671,DN191673-DN191677,DN191679-DN191682,
DN191685-DN191689,DN191693-DN191696,DN191698,DN191702,DN191703,DN191705-DN191712,
DN191714-DN191722,DN191724,DN191726,DN191728-DN191730,DN191733,DN191737-DN191739,DN191741,
DN191743-DN191745,DN191747-DN191749,DN191751,DN191753,DN191754,DN191757-DN191760,DN191763,
DN191765,DN191766,DN191769,DN191771-DN191773,DN191775,DN191777-DN191782,DN191784,
DN191787-DN191791,DN191793-DN191795,DN191797-DN191806,DN191808,DN191809,DN191811,DN191813,
DN191814,DN191816-DN191822,DN191826,DN191828,DN191832-DN191846,DN191848,DN191850-DN191853,
DN191856-DN191863,DN191866,DN191868,DN191869,DN191871,DN191872,DN191875,
DN191877-DN191890,DN191891-DN191895,DN191898-DN191900,DN191903-DN191910,DN191914-DN191919,
DN191921-DN191926,DN191930-DN191933,DN191937-DN191948,DN191950,DN191953,DN191955-DN191960,
DN191962,DN191967,DN191970-DN191972,DN191979,DN191984,DN191986-DN191989,DN191992-DN191994,
DN191997,DN192001-DN192005,DN192010,DN192012,DN192017,DN192019-DN192024,DN192026,DN192027,
DN192036,DN192037,DN192039-DN192042,DN192044,DN192046,DN192047,DN192049,DN192051,DN192052,
DN192054-DN192062,DN192064-DN192067,DN192069--DN192074,DN192076-DN192081,DN192084,
DN192085,DN192087,DN192088,DN192090-DN192092,DN192094,DN192095,DN192098-DN192100,
DN192103-DN192107,DN192110,DN192112,DN192116,DN192118,DN192120-DN192123,DN192126,DN192128,
DN192129,DN192131,DN192133-DN192135,DN192137,DN192138,DN192141,DN192142,DN192145,DN192146,
DN192148,DN192150,DN192151,DN192153,DN192157,DN192160,DN192163,DN192164,DN192169,
DN192170,DN192172,DN192174,DN192176,DN192179,DN192182-DN192184,DN192186,DN192188-DN192190,
DN192192,DN192193,DN192195,DN192199,DN192201-DN192205,DN192207,DN192208,DN192209,DN192211,
DN192214,DN192216,DN192219,DN192220,DN192223,DN192225,DN192226,DN192228,DN192230-DN192233,
DN192236-DN192238,DN192240,DN192243-DN192247,DN192251-DN192255,DN192257,DN192258,
DN192260-DN192263,DN192265-DN192269,DN192271,DN192273,DN192274,DN192276-DN192278,DN192280,
DN192281,DN192289,DN192290,DN192293-DN192295,DN192298-DN192300,DN192302-DN192311,
DN192314-DN192317,DN192319-DN192321,DN192323,DN192325,DN192327-DN192331,DN192334,
DN192336-DN192345,DN192347,DN192348,DN192353,DN192354,DN192357,DN192359,DN192360,
DN192362-DN192365,DN192367,DN192370,DN192374,DN192375,DN192377,DN192379,DN192381,DN192382,
DN192384,DN192385,DN192389-DN192391,DN192393,DN192398,DN192402,DN192404,DN192406-DN192409,
DN192414-DN192416,DN192418,DN192419,DN192421,DN192422,DN192424-DN192426,DN192429-DN192432,
DN192434,DN192438-DN192440,DN192447-DN192450,DN192452-DN192454,DN192456,DN192458-DN192461,
DN192464,DN192465,DN192467-DN192473,DN192475,DN192479,DN192483,DN192486-DN192489,DN192491,
DN192493,DN192495,DN192497,DN192499,DN192501,DN192503-DN192505,DN192507-DN192511,
DN192513-DN192515,DN192517,DN192519,DN192521,DN192524-DN192527,DN192529,DN192530,DN192532,
DN192536-DN192544,DN192546,DN192547,DN192549,DN192552,DN192554-DN192559,DN192561-DN192563,
DN192565,DN192566,DN192568-DN192571,DN192574,DN192576,DN960311-DN960316,DN960318,DN960327,
DN960329,DN960332,DN960333,DN960335,DN960341,DN960344,DN960350,DN960353-DN960356,
DN960359,DN960373,DN960379-DN960382,DN960384,DN960385,DN960388,DN960389,DN960391,DN960393,
DN960394,DN960402-DN960404,DN960407,DN960410,DN960412,DN960419,DN960422,DN960424,DN960426,
DN960427,DN960429-DN960431,DN960436,DN960438-DN960440,DN960442,DN960443,DN960446,DN960450,
DN960453,DN960458,DN960460-DN960462,DN960465,DN960466,DN960468,DN960471,DN960473,DN960476,
DN960478,DN960482,DN960483,DN960485,DN960487,DN960489,DN960491,DN960493,DN960494,
DN960496,DN960498-DN960501,DN960505,DN960506,DN960510,DN960511,DN960513-DN960518,
DN960521-DN960524,DN960527-DN960529,DN960532,DN960535-DN960538,DN960540,DN960541,DN960542,
DN960544,DN960546,DN960547,DN960549,DN960553,DN960555,DN960556,DN960557,DN960559,
DN960562,DN960563,DN960565,DN960568,DN960569,DN960571,DN960572,DN960575,DN960577,
DN960580,DN960582,DN960584,DN960587,DN960589,DN960592,DN960594,DN960595,DN960597,
DN960599,DN960601,DN960603,DN960604,DN960606,DN960611,DN960621,DN960628,DN960633,
DN960636,DN960639,DN960643,DN960651,DN960654,DN960657,DN960661,DN960665,DN960666,
DN960668,DN960670-DN960673,DN960675,DN960676,DN960685,DN960691,DN960693,DN960694,DN960695,
DN960698,DN960700,DN960701,DN960713,DN960716-DN960718,DN960720,DN960727,DN960728,DN960734,
DN960736-DN960740,DN960747,DN960750-DN960752,DN960757,DN960759,DN960764,DN960766-DN960770,
DN960773,DN960774,DN960776-DN960779,DN960783-DN960785,DN960793-DN960801,DN960803,
DN960809,DN960811-DN960813,DN960815-DN960817,DN960821,DN960825,DN960829,DN960832,DN960833,
DN960835,DN960837-DN960841,DN960843,DN960844,DN960846-DN960855,DN960858,DN960862,DN960864,
DN960868,DN960870-DN960874,DN960876,DN960878,DN960882,DN960883,DN960887,DN960894,
DN960896-DN960899,DN960901,DN960903,DN960904,DN960909,DN960915-DN960917,DN960925,
DN960930-DN960932,DN960934,DN960939,DN960940,DN960942,DN960944,DN960948,DN960952,DN960956,
DN960959,DN960961,DN960964,DN960969,DN960970,DN960974,DN960977,DN960979-DN960982,DN960984,
DN960986,DN960987,DN960991,DN960994,DN960995,DN960997,DN961007,DN961017-DN961020,DN961022,
DN961024,DN961027,DN961032,DN961034,DN961035,DN961039,DN961042,DN961043,DN961045,
DN961047,DN961048,DN961051,DN961053-DN961055,DN961057-DN961060,DN961064,DN961069,DN961072,
DN961074,DN961077,DN961079,DN961080,DN961084,DN961086,DN961091,DN961093,DN961097,
DN961101,DN961105,DN961107,DN961108,DN961110-DN961112,DN961114,DN961116,DN961120,
DN961122-DN961126,DN961129,DN961132-DN961146,DN961148,DN961151,DN961152,DN961154,DN961156,
DN961161,DN961164-DN961166,DN961169,DN961170,DN961172-DN961177,DN961180-DN961183,DN961185,
DN961186,DN961188,DN961190,DN961194,DN961200,DN961201,DN961203,DN961204,DN961208,
DN961211-DN961213,DN961215,DN961217,DN961219-DN961223,DN961225-DN961227,DN961229,DN961231,
DN961234,DN961238,DN961240,DN961242,DN961245,DN961247,DN961249,DN961253-DN961255,DN961261,
DN961262,DN961265,DN961268,DN961269,DN961271-DN961274,DN961278,DN961279,DN961283-DN961285,
DN961293,DN961295-DN961297,DN961300,DN961303,DN961305-DN961308,DN961311,DN961316,DN961318,
DN961320-DN961322,DN961324-DN961326,DN961328,DN961335,DN961337-DN961339,DN961341,DN961343,
DN961344,DN961348,DN961350-DN961353,DN961356,DN961359,DN961360,DN961362,DN961366,DN961370,
DN961371,DN961375,DN961376,DN961378,DN961380,DN961385,DN961386,DN961390,DN961392,
DN961393,DN961398-DN961402,DN961405-DN961407,DN961417,DN961420-DN961425,DN961428,DN961429,
DN961432,DN961433,DN961435,DN961437,DN961439,DN961442,DN961448,DN961452-DN961457,
DN961460-DN961462,DN961465,DN961466,DN961469,DN961470,DN961477-DN961479,DN961482-DN961495,
DN961497,DN961498,DN961502-DN961504,DN961508,DN961514,DN961519,DN961521,DN961522,DN961524,
DN961525,DN961527,DN961532,DN961533,DN961536,DN961538,DN961542,DN961543,DN961545,
DN961547,DN961553,DN961555,DN961558-DN961560,DN961562,DN961567-DN961571,DN961574,
DN961576-DN961578,DN961580,DN961584,DN961585,DN961587,DN961588,DN961592,DN961597,DN961600,
DN961601,DN961603,DN961605,DN961606,DN961609,DN961613,DN961614,DN961616,DN961619,
DN961624,DN961625,DN961628-DN961634,DN961637,DN961639,DN961641,DN961642,DN961645,DN961649,
DN961650,DN961653-DN961658,DN961661,DN961663,DN961664,DN961666,DN961668,DN961669,DN961671,
DN961682-DN961686,DN961688,DN961692-DN961701,DN961705,DN961706,DN961708,DN961714,
DN961717-DN961719,DN961724,DN961726,DN961727,DN961729,DN961731-DN961736,DN961738,DN961740,
DN961743,DN961744,DN961746-DN961749,DN961751-DN961754,DN961757-DN961760,DN961762,DN961764,
DN961766-DN961768,DN961772-DN961774,DN961776,DN961777,DN961779,DN961781,DN961784,DN961786,
DN961788,DN961789,DN961793,DN961794,DN961798,DN961801-DN961805,DN961807,DN961808,DN961810,
DN961811,DN961813,DN961814,DN961816-DN961820,DN961826,DN961828,DN961830,DN961832-DN961836,
DN961838-DN961840,DN961844,DN961846,DN961847,DN961849,DN961852,DN961857,DN961860,DN961861,
DN961863-DN961870,DN961873,DN961874,DN961878,DN961881,DN961884,DN961888,DN961891,DN961894,
DN961897,DN961900-DN961902,DN961907-DN961910,DN961914,DN961916,DN961918,DN961920-DN961925,
DN961929,DN961931,DN961940,DN961943-DN961945,DN961950,DN961952,DN961959,DN961961,DN961966,
DN961967,DN961970,DN961972,DN961974,DN961980,DN961982,DN961985,DN961987,DN961991,
DN961992,DN962004,DN962011,DN962012,DN962017,DN962021,DN962025,DN962034,DN962035,
DN962039,DN962042,DN962045-DN962048,DN962053,DN962057,DN962059,DN962061,DN962067,DN962069,
DN962073,DN962074,DN962077,DN962079-DN962084,DN962086,DN962088,DN962093,DN962096,DN962099,
DN962100,DN962104,DN962105,DN962107,DN962114,DN962121,DN962122,DN962124,DN962126,
DN962133-DN962135,DN962139,DN962141-DN962143,DN962146,DN962147,DN962152,DN962153,
DN962156-DN962158,DN962165,DN962166,DN962171,DN962173,DN962175,DN962177,DN962178,DN962192,
DN962194,DN962196,DN962197,DN962201,DN962203,DN962207,DN962208,DN962211,DN962214,
DN962215,DN962222-DN962224,DN962228,DN962231,DN962233,DN962236,DN962241,DN962244,DN962247,
DN962252,DN962258,DN962264,DN962268-DN962271,DN962276-DN962289,DN962293-DN962298,
DN962301-DN962304,DN962307,DN962311,DN962312,DN962315,DN962318,DN962319,DN962324,DN962328,
DN962331,DN962334,DN962337,DN962339,DN962347,DN962350,DN962354-DN962356,DN962359,
DN962364-DN962366,DN962368,DN962370,DN962372,DN962373,DN962375,DN962380-DN962382,DN962384,
DN962386,DN962389,DN962391,DN962393,DN962395,DN962396,DN962398,DN962400,DN962401,
DN962403,DN962406-DN962408,DN962412-DN962415,DN962418,DN962423,DN962426,DN962432,
DN962435-DN962437,DN962440,DN962441,DN962445,DN962447-DN962453,DN962457-DN962459,
DN962463-DN962465,DN962467,DN962474,DN962476-DN962479,DN962482-DN962484,DN962492,DN962494,
DN962498-DN962502,DN962504,DN962505,DN962507,DN962510,DN962512,DN962513,DN962517,DN962518,
DN962520-DN962523,DN962527,DN962528,DN962533-DN962535,DN962537-DN962542,DN962545,DN962548,
DN962549,DN962554,DN962557,DN962562,DN962563,DN962569,DN962572-DN962576,DN962578-DN962580,
DN962582,DN962585,DN962586,DN962589,DN962591,DN962597-DN962602,DN962605,DN962606,DN962609,
DN962610,DN962612,DN962614-DN962617,DN962619,DN962624-DN962626,DN962631,DN962632,DN962636,
DN962644-DN962647,DN962650,DN962652,DN962653,DN962655,DN962657-DN962660,DN962663,DN962664,
DN962668,DN962669,DN962671,DN962673,DN962674,DN962677,DN962679-DN962682,DN962684,DN962686,
DN962687,DN962690,DN962692-DN962694,DN962699,DN962700,DN962703,DN962704,DN962707,DN962708,
DN962711,DN962712,DN962715,DN962718,DN962722,DN962723,DN962725,DN962728,DN962731,
DN962732,DN962735,DN962736,DN962739,DN962744-DN962749,DN962756,DN962758,DN962759,DN962761,
DN962762,DN962766,DN962770,DN962776,DN962779,DN962781,DN962783,DN962787,DN962788,
DN962790,DN962792,DN962793,DN962801,DN962803,DN962809,DN962811,DN962814,DN962815,
DN962818-DN962822,DN962830,DN962831,DN962832,DN962834,DN962837,DN962840,DN962841,DN962843,
DN962844,DN962852,DN962854,DN962857-DN962862,DN962864,DN962865,DN962867,DN962869,
DN962870-DN962877,DN962879-DN962882,DN962884,DN962886,DN962888,DN962893,DN962895,
DN962897-DN962900,DN962904,DN962906,DN962909,DN962911,DN962917,DN962922,DN962923,DN962925,
DN962927,DN962930-DN962933,DN962935,DN962936,DN962938,DN962939,DN962941,DN962942,DN962945,
-DN962948,DN962950,DN962952,DN962956,DN962957,DN962962,DN962968,DN962979,DN962981,
DN962982,DN962984,DN962985,DN962987-DN962989,DN962991,DN962992,DN962994,DN962995,DN962997,
DN962998,DN963001-DN963015,DN963021,DN963022,DN963025,DN963027,DN963028,DN963032,DN963035,
DN963037,DN963045,DN963046,DN963050,DN963052,DN963055,DN963056,DN963060,DN963064,
DN963065,DN963069,DN963073,DN963080,DN963085,DN963088,DN963090,DN963092-DN963095,
DN963100-DN963103,DN963111,DN963113,DN963114,DN963117,DN963119,DN963120,DN963128,DN963132,
DN963138,DN963142,DN963144,DN963147,DN963151,DN963156,DN963160,DN963161,DN963164,
DN963167,DN963175,DN963179,DN963180,DN963182,DN963185,DN963190,DN963193,DN963197,
DN963203,DN963213-DN963215,DN963217,DN963225,DN963227,DN963228,DN963233,DN963234,DN963237,
DN963240,DN963244,DN963249,DN963260,DN963266,DN963267,DN963270-DN963272,DN963278-DN963282,
DN963284-DN963286,DN963290,DN963291,DN963294,DN963296,DN963298,DN963299,DN963301,DN963308,
DN963311,DN963316,DN963317,DN963319,DN963325,DN963327-DN963329,DN963331,DN963333-DN963335,
DN963339-DN963341,DN963343,DN963350,DN963355,DN963357-DN963359,DN963361,DN963362,DN963367,
DN963373-DN963377,DN963379,DN963383,DN963386,DN963387,DN963388,DN963394-DN963396,DN963399,
DN963401,DN963403,DN963404,DN963406,DN963407,DN963411,DN963414,DN963421,DN963429,
DN963431,DN963434,DN963439-DN963442,DN963449,DN963461,DN963464,DN963465,DN963467,DN963477,
DN963479-DN963483,DN963490,DN963492,DN963493,DN963497,DN963504,DN963507,DN963508,
DN963510-DN963515,DN963521,DN963523,DN963524,DN963529,DN963536,DN963538,DN963539,DN963542,
DN963548-DN963550,DN963552,DN963555-DN963558,DN963561,DN963563,DN963565,DN963568,DN963571,
DN963578,DN963580,DN963581,DN963584,DN963589,DN963593,DN963595,DN963599,DN963600,
DN963602,DN963608,DN963615,DN963620-DN963623,DN963632,DN963633,DN963636,DN963637,
DN963644-DN963648,DN963650-DN963652,DN963655,DN963657,DN963658,DN963660,DN963663,DN963667,
DN963672,DN963674,DN963676,DN963683,DN963685-DN963690,DN963692,DN963696,DN963699,DN963700,
DN963702,DN963703,DN963712,DN963715,DN963720,DN963721,DN963724-DN963726,DN963729,DN963731,
DN963733,DN963735,DN963736,DN963737,DN963741,DN963743,DN963744,DN963749,DN963750,
DN963755,DN963764,DN963766,DN963774,DN963780,DN963782,DN963784-DN963786,DN963795,DN963799,
DN963800,DN963802,DN963806,DN963808,DN963810,DN963813,DN963821,DN963825,DN963826,
DN963830-DN963832,DN963835,DN963838,DN963839,DN963842,DN963844-DN963846,DN963852,DN963853,
DN963856-DN963858,DN963860,DN963863-DN963866,DN963873,DN963877,DN963879,DN963881,DN963888,
DN963889,DN963891,DN963895,DN963898,DN963899,DN963903,DN963905,DN963913,DN963917,
DN963920,DN963921,DN963923,DN963929,DN963932,DN963935-DN963940,DN963943,DN963945,DN963946,
DN963948,DN963954,DN963955,DN963957,DN963966,DN963970,DN963973,DN963977,DN963978,
DN963988,DN963989,DN963993,DN963999,DN964000,DN964003,DN964004,DN964006,DN964013,
DN964015,DN964017,DN964019,DN964024,DN964027,DN964031,DN964033,DN964036,DN964038,
DN964043,DN964048-DN964052,DN964057-DN964062,DN964068,DN964071,DN964073,DN964078,DN964080,
DN964081,DN964083,DN964089,DN964092,DN964093,DN964098-DN964100,DN964103,DN964108,DN964109,
DN964112,DN964115,DN964118,DN964119,DN964125,DN964130-DN964133,DN964136,DN964144-DN964146,
DN964150,DN964154,DN964157,DN964158,DN964162,DN964164,DN964168,DN964173,DN964175,
DN964178,DN964185,DN964187,DN964189,DN964194,DN964195,DN964197,DN964201,DN964202,
DN964205,DN964206,DN964212,DN964214,DN964217,DN964220-DN964224,DN964230,DN964232,DN964234,
DN964235,DN964240,DN964242,DN964243,DN964247,DN964254,DN964256,DN964259,DN964261,
DN964267,DN964271,DN964272,DN964277,DN964278,DN964285,DN964286,DN964288,DN964292,
DN964295,DN964298,DN964300,DN964303,DN964305,DN964306,DN964308,DN964310,DN964320,
DN964334,DN964340,DN964345,DN964347,DN964348,DN964352-DN964355,DN964360,DN964361,DN964362,
DN964364,DN964367,DN964372,DN964374,DN964376,DN964378,DN964386-DN964392,DN964394,DN964396,
DN964402,DN964404,DN964406,DN964409-DN964416,DN964419,DN964420,DN964422,DN964426,
DN964430-DN964436,DN964442,DN964450,DN964452,DN964455,DN964456,DN964462,DN964464,DN964466,
DN964469,DN964472,DN964473,DN964478,DN964481,DN964484-DN964488,DN964492-DN964495,DN964498,
DN964502-DN964504,DN964507,DN964509,DN964512,DN964514-DN964516,DN964518,DN964521-DN964524,
DN964527-DN964529,DN964531,DN964532,DN964534,DN964536,DN964537,DN964538,DN964539,DN964543,
DN964544,DN964547,DN964550,DN964551,DN964552,DN964554,DN964559,DN964560,DN964562,
DN964563-DN964571,DN964574,DN964576,DN964578,DN964580,DN964582,DN964585-DN964594,
DN964598-DN964602,DN964609-DN964612,DN964614-DN964619,DN964622,DN964623,DN964627,DN964628,
DN964630,DN964631,DN964633,DN964635,DN964638,DN964640,DN964642,DN964648,DN964651,
DN964654,DN964656,DN964658-DN964662,DN964664,DN964666,DN964667,DN964669-DN964675,DN964681,
DN964682-DN964684,DN964688,DN964690,DN964693,DN964695-DN964697,DN964699,DN964701,DN964703,
DN964709,DN964712,DN964719,DN964722,DN964723,DN964729,DN964732,DN964733,DN964738-DN964742,
DN964745,DN964747,DN964749,DN964751,DN964752,DN964756,DN964757,DN964759,DN964760,
DN964763,DN964770,DN964771,DN964774-DN964777,DN964781,DN964783-DN964787,DN964791,DN964796,
DN964797,DN964800,DN964804,DN964805,DN964807,DN964809-DN964813,DN964816,DN964820-DN964827,
DN964831-DN964833,DN964835,DN964837-DN964840,DN964844,DN964849-DN964851,DN964854-DN964856,
DN964858,DN964860,DN964862,DN964865,DN964868,DN964870,DN964874,DN964882,DN964885,
DN964886,DN964888,DN964889,DN964896,DN964901,DN964903,DN964910-DN964912,DN964916,DN964919,
DN964921,DN964926,DN964927,DN964930,DN964932,DN964936,DN964938,DN964939,DN964943,
DN964944,DN964947,DN964948,DN964950,DN964953,DN964954,DN964957,DN964959,DN964962,
DN964967,DN964972,DN964973,DN964975,DN964979,DN964984,DN964985,DN964994,DN964996,
DN965001,DN965004,DN965009,DN965013,DN965017,DN965018-DN965021,DN965029,DN965031,DN965043,
DN965055,DN965056,DN965061,DN965063,DN965065-DN965070,DN965080,DN965092,DN965096-DN965099,
DN965102,DN965105,DN965108,DN965111,DN965113,DN965119,DN965121,DN965126-DN965128,DN965131,
DN965133-DN965135,DN965137,DN965140,DN965142,DN965144,DN965147-DN965152,DN965154,DN965157,
DN965158,DN965160,DN965165,DN965168,DN965175,DN965182,DN965183,DN965185,DN965194,
DN965197,DN965198,DN965207,DN965212,DN965213,DN965220,DN965224,DN965236,DN965244,
DN965245,DN965258,DN965267,DN965269-DN965272,DN965275-DN965278,DN965280,DN965282,DN965284,
DN965290,DN965291,DN965293,DN965294,DN965298,DN965301,DN965307,DN965308,DN965313,
DN965315,DN965321,DN965323,DN965327,DN965331,DN965335,DN965337,DN965339-DN965343,DN965348,
DN965352,DN965353,DN965357-DN965359,DN965368,DN965371,DN965373-DN965377,DN965382,DN965388,
DN965390,DN965392,DN965394-DN965396,DN965398,DN965400,DN965402,DN965404,DN965405,
DN965410-DN965412,DN965414,DN965421,DN965423-DN965425,DN965428,DN965431-DN965433,DN965435,
DN965436,DN965439-DN965445,DN965447,DN965448,DN965450-DN965452,DN965454-DN965456,DN965459,
DN965461,DN965463,DN965465,DN965468-DN965470,DN965473,DN965476-DN965485,DN965487-DN965495,
DN965497,DN965498,DN965500,DN965503,DN965506,DN965511,DN965513,DN965515,DN965517-DN965519,
DN965522,DN965523,DN965525,DN965528,DN965530,DN965532,DN965535,DN965538-DN965540,DN965542,
DN965546-DN965551,DN965558,DN965559,DN965561,DN965563,DN965564,DN965568,DN965572,DN965578,
DN965579,DN965581-DN965583,DN965595,DN965596,DN965598,DN965600,DN96560 1,DN965603,DN965604,
DN965606,DN965608,DN965609,DN965611,DN965613,DN965621,DN965623,DN965627,DN965632,
DN965633,DN965637,DN965647,DN965654,DN965658,DN965660,DN965661,DN965663,DN965665,
DN965674,DN965676,DN965679,DN965681,DN965684,DN965686,DN965690,DN965691,DN965694,
DN965695,DN965697,DN965699,DN965702,DN965704,DN965706,DN965707,DN965709,DN965719,
DN965721,DN965735,DN965736,DN965737,DN965742,DN965743,DN965749-DN965751,DN965760,DN965767,
DN965769,DN965774,DN965776,DN965777,DN965786,DN965790,DN965791,DN965793,DN965796,
DN965801,DN965804,DN965807-DN965812,DN965815,DN965821,DN965831,DN965833,DN965835,DN965837,
DN965838,DN965847,DN965848,DN965850,DN965853,DN965855,DN965858,DN965860,DN965864,
DN965866,DN965867,DN965871,DN965873,DN965875,DN965879,DN965882,DN965883,DN965886,
DN965889,DN965895-DN965897,DN965900,DN965901,DN965903,DN965905,DN965908,DN965909,DN965914,
DN965915,DN965918,DN965919-DN965925,DN965934-DN965938,DN965942,DN965946,DN965959,DN965962,
DN965964,DN965966,DN965970,DN965971,DN965975-DN965977,DN965979,DN965980,DN965984,DN965985,
DN965987,DN965988,DN965997-DN965999,DN966006,DN966010,DN966013,DN966017,DN966019,DN966021,
DN966023,DN966025,DN966031,DN966033,DN966035,DN966041,DN966050,DN966052,DN966054,
DN966057,DN966058,DN966064,DN966066,DN966072,DN966073,DN966075,DN966088,DN966089,
DN966102,DN966112,DN966116,DN966121,DN966122,DN966127,DN966133,DN966135,DN966140,
DN966142,DN966147,DN966157,DN966159,DN966160,DN966161,DN966164,DN966166,DN966170,
DN966177,DN966179,DN966180,DN966184,DN966186,DN966191,DN966196,DN966201,DN966203,
DN966206,DN966208,DN966211,DN966213,DN966214,DN966216,DN966217,DN966221,DN966223,
DN966228,DN966231,DN966233,DN966241,DN966244,DN966250,DN966251,DN966257,DN966259,
DN966260,DN966268,DN966272,DN966286,DN966289,DN966291,DN966293,DN966295,DN966297,
DN966300,DN966301,DN966309,DN966315,DN966325,DN966330,DN966331-DN966356,DN966358,DN966364,
DN966365,DN966367,DN966370,DN966380,DN966383,DN966395,DN966400,DN966405,DN966406,
DN966410,DN966414-DN966417,DN966424,DN966427,DN966436,DN966438,DN966451,DN966453,DN966464,
DN966467,DN966468,DN966470,DN966472,DN966475,DN966491,DN966496,DN966498,DN966501,
DN966504,DN966505,DN966512,DN966513,DN966524,DN966528,DN966530,DN966537,DN966538,
DN966540,DN966543,DN966550,DN966552,DN966553,DN966555,DN966556,DN966558,DN966562,
DN966566-DN966568,DN966573-DN966576,DN966579,DN966587,DN966591,DN966593,DN966596,DN966599。
2、一种权利要求1所述cDNA芯片的制备方法,包括以下步骤:
1)构建受软腐欧文氏(Erwinia carotovora subsp.carotovora)感染大白菜的抑制消减cDNA文库以及大白菜莲座期和结球期幼嫩叶片的cDNA文库;
2)自步骤1)中构建的cDNA文库中挑选非冗余序列制作探针,所述探针为序列表中SEQID №:1-221的DNA序列,以及3885条具有下述GenBank登录号的DNA序列:
CA991436,CA991442,CA991443,CA991447-CA991450,CA991452-CA991458,CA991460,CA991462,CA991463,
CA991465-CA991470,CA991473-CA991482,CA991484-CA991493,CA991495-CA991500,CA991502,CA991503,
CA991505,CA991508,CA991510-CA991522,CA991524,CA991525,CA991527,CA991528,CA991530,CA991532,
CA991534,CA991536-CA991540,CA991542,CA991544-CA991546,CA991548,CA991549,CA991551-CA991566,
CA991568-CA991572,CA991574-CA991577,CA991579,CA991581,CA991582,CA991585-CA991603,
CA991606-CA991610,CA991616-CA991654,CA991666-CA991668,CA991671,CA991673-CA991675,
CA991678-CA991681,CA991686-CA991693,CA991698-CA991700,CA991705-CA991707,CA991710,
CA991714,CA991715,CA991718-CA991720,CA991723,CA991725-CA991727,CA991729-CA991733,CA991735,
CA991737-CA991763,CA991770-CA991773,CA991776-CA991778,CA991781,CA991784-CA991790,CA991792,
CA991793,CA991795,CA991797-CA991804,CA991806-CA991808,CA991814,CA991817-CA991822,
CA991826-CA991832,CA991834,CA991835,CA991839-CA991842,CA991848-CA991852,CA991854,
CA991855,CA991857,CA991858-CA991860,CA991866,CA991869,CA991872-CA991875,CA991877,
CA991879-CA991882,CA991887,CA991892,CA991895-CA991898,CA991900,CA991901,
CA991903-CA991905,CA991907,CA991909,CA991911,CA991912,CA991915-CA991927,CA991930,CA991934,
CA991936-CA991944,CA991946-CA991954,CA991960,CA991961,CA991964,CA991966,CA991968-CA991973,
CA991976-CA991984,CA991987,CA991989,CA991991-CA991995,CA992000-CA992002,CA992005,CA992007,
CA992008,CA992010-CA992012,CA992015,CA992017-CA992025,CA992028,CA992032,CA992033,CA992037,
CA992041-CA992045,CA992053-CA992057,CA992061,CA992062,CA992065-CA992069,CA992072-CA992076,
CA992080,CA992083,CA992086,CA992088-CA992091,CA992093-CA992097,CA992101,CA992105,CA992114,
CA992118,CA992120-CA992122,CA992125,CA992127,CA992130,CA992132,CA992138-CA992146,
CA992148,CA992150,CA992155,CA992159,CA992161,CA992163,CA992169-CA992172,CA992175-CA992178,
CA992 182-CA992192,CA992195,CA992198,CA992202,CA992203,CA992206-CA992208,CA992212,CA992214,
CA992215,CA992218,CA992219,CA992221,CA992223-CA992225,CA992227,CA992229,CA992232-CA992234,
CA992237,CA992241,CA992247-CA992249,CA992253,CA992254,CA992259-CA992267,CA992272,CA992273,
CA992277-CA992283,CA992287-CA992289,CA992292-CA992296,CA992299,CA992303-CA992305,CA992308,
CA992310,CA992315,CA992319,CA992324-CA992333,CA992336,CA992338-CA992350,CA992355,CA992356,
CA992359-CA992366,CA992373,CA992376,CA992378,CA992380,CA992385-CA992388,CA992391,
CA992392,CA992394,CA992396,CA992400,CA992401,CA992406-CA992418,CA992421-CA992424,CA992428,
CA992431,CA992432,CA992434,CA992436,CA992439-CA992442,CA992445-CA992448,CA992450,CA992451,
CA992458-CA992461,CA992464-CA992473,CA992476-CA992481,CA992484,CA992487,CA992488,CA992492,
CA992496,CA992498-CA992504,CA992507,CA992508,CA992510-CA992513,CA992516-CA992524,
CA992530-CA992538,CA992540,CA992542-CA992546,CA992553-CA992569,CA992573-CA992579,
CA992583-CA992585,DN191370,DN191373-DN191379,DN191385,DN191388-DN191391,DN191394-DN191397,
DN191399-DN191401,DN191403,DN191406-DN191413,DN191417,DN191419,DN191422,DN191424,DN191429,
DN191430,DN191433-DN191435,DN191438-DN191443,DN191445,DN191450,DN191453,DN191456,
DN191458-DN191462,DN191464,DN191467,DN191468,DN191471,DN191473,DN191474,DN191476,DN191479,
DN191483,DN191485-DN191488,DN191491,DN191492,DN191497,DN191498,DN191502,DN191503,DN191506,
DN191507,DN191510-DN191526,DN191530-DN191532,DN191534-DN191536,DN191538,DN191543,DN191544,
DN191546,DN191548-DN191554,DN191556,DN191559-DN191561,DN191563,DN191564,DN191570,DN191572,
DN191573,DN191575-DN191577,DN191579,DN191582-DN191586,DN191588-DN191590,DN191594,DN191597,
DN191599,DN191603-DN191607,DN191609-DN191618,DN191620-DN191626,DN191629,DN191631-DN191634,
DN191636-DN191638,DN191642-DN191644,DN191647,DN191649,DN191651,DN191653-DN191655,DN191659,
DN191662,DN191665,DN191666,DN191670,DN191671,DN191673-DN191677,DN191679-DN191682,
DN191685-DN191689,DN191693-DN191696,DN191698,DN191702,DN191703,DN191705-DN191712,
DN191714-DN191722,DN191724,DN191726,DN191728-DN191730,DN191733,DN191737-DN191739,DN191741,
DN191743-DN191745,DN191747-DN191749,DN191751,DN191753,DN191754,DN191757-DN191760,DN191763,
DN191765,DN191766,DN191769,DN191771-DN191773,DN191775,DN191777-DN191782,DN191784,
DN191787-DN191791,DN191793-DN191795,DN191797-DN191806,DN191808,DN191809,DN191811,DN191813,
DN191814,DN191816-DN191822,DN191826,DN191828,DN191832-DN191846,DN191848,DN191850-DN191853,
DN191856-DN191863,DN191866,DN191868,DN191869,DN191871,DN191872,DN191875,
DN191877-DN191890,DN191891-DN191895,DN191898-DN191900,DN191903-DN191910,DN191914-DN191919,
DN191921-DN191926,DN191930-DN191933,DN191937-DN191948,DN191950,DN191953,DN191955-DN191960,
DN191962,DN191967,DN191970-DN191972,DN191979,DN191984,DN191986-DN191989,DN191992-DN191994,
DN191997,DN192001-DN192005,DN192010,DN192012,DN192017,DN192019-DN192024,DN192026,DN192027,
DN192036,DN192037,DN192039-DN192042,DN192044,DN192046,DN192047,DN192049,DN192051,DN192052,
DN192054-DN192062,DN192064-DN192067,DN192069-DN192074,DN192076-DN192081,DN192084,
DN192085,DN192087,DN192088,DN192090-DN192092,DN192094,DN192095,DN192098-DN192100,
DN192103-DN192107,DN192110,DN192112,DN192116,DN192118,DN192120-DN192123,DN192126,DN192128,
DN192129,DN192131,DN192133-DN192135,DN192137,DN192138,DN192141,DN192142,DN192145,DN192146,
DN192148,DN192150,DN192151,DN192153,DN192157,DN192160,DN192163,DN192164,DN192169,
DN192170,DN192172,DN192174,DN192176,DN192179,DN192182-DN192184,DN192186,DN192188-DN192190,
DN192192,DN192193,DN192195,DN192199,DN192201-DN192205,DN192207,DN192208,DN192209,DN192211,
DN192214,DN192216,DN192219,DN192220,DN192223,DN192225,DN192226,DN192228,DN192230-DN192233,
DN192236-DN192238,DN192240,DN192243-DN192247,DN192251-DN192255,DN192257,DN192258,
DN192260-DN192263,DN192265-DN192269,DN192271,DN192273,DN192274,DN192276-DN192278,DN192280,
DN192281,DN192289,DN192290,DN192293-DN192295,DN192298-DN192300,DN192302-DN192311,
DN192314-DN192317,DN192319-DN192321,DN192323,DN192325,DN192327-DN192331,DN192334,
DN192336-DN192345,DN192347,DN192348,DN192353,DN192354,DN192357,DN192359,DN192360,
DN192362-DN192365,DN192367,DN192370,DN192374,DN192375,DN192377,DN192379,DN192381,DN192382,
DN192384,DN192385,DN192389-DN192391,DN192393,DN192398,DN192402,DN192404,DN192406-DN192409,
DN192414-DN192416,DN192418,DN192419,DN192421,DN192422,DN192424-DN192426,DN192429-DN192432,
DN192434,DN192438-DN192440,DN192447-DN192450,DN192452-DN192454,DN192456,DN192458-DN192461,
DN192464,DN192465,DN192467-DN192473,DN192475,DN192479,DN192483,DN192486-DN192489,DN192491,
DN192493,DN192495,DN192497,DN192499,DN192501,DN192503-DN192505,DN192507-DN192511,
DN192513-DN192515,DN192517,DN192519,DN192521,DN192524-DN192527,DN192529,DN192530,DN192532,
DN192536-DN192544,DN192546,DN192547,DN192549,DN192552,DN192554-DN192559,DN192561-DN192563,
DN192565,DN192566,DN192568-DN192571,DN192574,DN192576,DN960311-DN960316,DN960318,DN960327,
DN960329,DN960332,DN960333,DN960335,DN960341,DN960344,DN960350,DN960353-DN960356,
DN960359,DN960373,DN960379-DN960382,DN960384,DN960385,DN960388,DN960389,DN960391,DN960393,
DN960394,DN960402-DN960404,DN960407,DN960410,DN960412,DN960419,DN960422,DN960424,DN960426,
DN960427,DN960429-DN960431,DN960436,DN960438-DN960440,DN960442,DN960443,DN960446,DN960450,
DN960453,DN960458,DN960460-DN960462,DN960465,DN960466,DN960468,DN960471,DN960473,DN960476,
DN960478,DN960482,DN960483,DN960485,DN960487,DN960489,DN960491,DN960493,DN960494,
DN960496,DN960498-DN960501,DN960505,DN960506,DN960510,DN960511,DN960513-DN960518,
DN960521-DN960524,DN960527-DN960529,DN960532,DN960535-DN960538,DN960540,DN960541,DN960542,
DN960544,DN960546,DN960547,DN960549,DN960553,DN960555,DN960556,DN960557,DN960559,
DN960562,DN960563,DN960565,DN960568,DN960569,DN960571,DN960572,DN960575,DN960577,
DN960580,DN960582,DN960584,DN960587,DN960589,DN960592,DN960594,DN960595,DN960597,
DN960599,DN960601,DN960603,DN960604,DN960606,DN960611,DN960621,DN960628,DN960633,
DN960636,DN960639,DN960643,DN960651,DN960654,DN960657,DN960661,DN960665,DN960666,
DN960668,DN960670-DN960673,DN960675,DN960676,DN960685,DN960691,DN960693,DN960694,DN960695,
DN960698,DN960700,DN960701,DN960713,DN960716-DN960718,DN960720,DN960727,DN960728,DN960734,
DN960736-DN960740,DN960747,DN960750-DN960752,DN960757,DN960759,DN960764,DN960766-DN960770,
DN960773,DN960774,DN960776-DN960779,DN960783-DN960785,DN960793-DN960801,DN960803,
DN960809,DN960811-DN960813,DN960815-DN960817,DN960821,DN960825,DN960829,DN960832,DN960833,
DN960835,DN960837-DN960841,DN960843,DN960844,DN960846-DN960855,DN960858,DN960862,DN960864,
DN960868,DN960870-DN960874,DN960876,DN960878,DN960882,DN960883,DN960887,DN960894,
DN960896-DN960899,DN960901,DN960903,DN960904,DN960909,DN960915-DN960917,DN960925,
DN960930-DN960932,DN960934,DN960939,DN960940,DN960942,DN960944,DN960948,DN960952,DN960956,
DN960959,DN960961,DN960964,DN960969,DN960970,DN960974,DN960977,DN960979-DN960982,DN960984,
DN960986,DN960987,DN960991,DN960994,DN960995,DN960997,DN961007,DN961017-DN961020,DN961022,
DN961024,DN961027,DN961032,DN961034,DN961035,DN961039,DN961042,DN961043,DN961045,
DN961047,DN961048,DN961051,DN961053-DN961055,DN961057-DN961060,DN961064,DN961069,DN961072,
DN961074,DN961077,DN961079,DN961080,DN961084,DN961086,DN961091,DN961093,DN961097,
DN961101,DN961105,DN961107,DN961108,DN961110-DN961112,DN961114,DN961116,DN961120,
DN961122-DN961126,DN961129,DN961132-DN961146,DN961148,DN961151,DN961152,DN961154,DN961156,
DN961161,DN961164-DN961166,DN961169,DN961170,DN961172-DN961177,DN961180-DN961183,DN961185,
DN961186,DN961188,DN961190,DN961194,DN961200,DN961201,DN961203,DN961204,DN961208,
DN961211-DN961213,DN961215,DN961217,DN961219-DN961223,DN961225-DN961227,DN961229,DN961231,
DN961234,DN961238,DN961240,DN961242,DN961245,DN961247,DN961249,DN961253-DN961255,DN961261,
DN961262,DN961265,DN961268,DN961269,DN961271-DN961274,DN961278,DN961279,DN961283-DN961285,
DN961293,DN961295-DN961297,DN961300,DN961303,DN961305-DN961308,DN961311,DN961316,DN961318,
DN961320-DN961322,DN961324-DN961326,DN961328,DN961335,DN961337-DN961339,DN961341,DN961343,
DN961344,DN961348,DN961350-DN961353,DN961356,DN961359,DN961360,DN961362,DN961366,DN961370,
DN961371,DN961375,DN961376,DN961378,DN961380,DN961385,DN961386,DN961390,DN961392,
DN961393,DN961398-DN961402,DN961405-DN961407,DN961417,DN961420-DN961425,DN961428,DN961429,
DN961432,DN961433,DN961435,DN961437,DN961439,DN961442,DN961448,DN961452-DN961457,
DN961460-DN961462,DN961465,DN961466,DN961469,DN961470,DN961477-DN961479,DN961482-DN961495,
DN961497,DN961498,DN961502-DN961504,DN961508,DN961514,DN961519,DN961521,DN961522,DN961524,
DN961525,DN961527,DN961532,DN961533,DN961536,DN961538,DN961542,DN961543,DN961545,
DN961547,DN961553,DN961555,DN961558-DN961560,DN961562,DN961567-DN961571,DN961574,
DN961576-DN961578,DN961580,DN961584,DN961585,DN961587,DN961588,DN961592,DN961597,DN961600,
DN961601,DN961603,DN961605,DN961606,DN961609,DN961613,DN961614,DN961616,DN961619,
DN961624,DN961625,DN961628-DN961634,DN961637,DN961639,DN961641,DN961642,DN961645,DN961649,
DN961650,DN961653-DN961658,DN961661,DN961663,DN961664,DN961666,DN961668,DN961669,DN9616711,
DN961682-DN961686,DN961688,DN961692-DN961701,DN961705,DN961706,DN961708,DN961714,
DN961717-DN961719,DN961724,DN961726,DN961727,DN961729,DN961731-DN961736,DN961738,DN961740,
DN961743,DN961744,DN961746-DN961749,DN961751-DN961754,DN961757-DN961760,DN961762,DN961764,
DN961766-DN961768,DN961772-DN961774,DN961776,DN961777,DN961779,DN961781,DN961784,DN961786,
DN961788,DN961789,DN961793,DN961794,DN961798,DN961801-DN961805,DN961807,DN961808,DN961810,
DN961811,DN961813,DN961814,DN961816-DN961820,DN961826,DN961828,DN961830,DN961832-DN961836,
DN961838-DN961840,DN961844,DN961846,DN961847,DN961849,DN961852,DN961857,DN961860,DN961861,
DN961863-DN961870,DN961873,DN961874,DN961878,DN961881,DN961884,DN961888,DN961891,DN961894,
DN961897,DN961900-DN961902,DN961907-DN961910,DN961914,DN961916,DN961918,DN961920-DN961925,
DN961929,DN961931,DN961940,DN961943-DN961945,DN961950,DN961952,DN961959,DN961961,DN961966,
DN961967,DN961970,DN961972,DN961974,DN961980,DN961982,DN961985,DN961987,DN961991,
DN961992,DN962004,DN962011,DN962012,DN962017,DN962021,DN962025,DN962034,DN962035,
DN962039,DN962042,DN962045-DN962048,DN962053,DN962057,DN962059,DN96206 1,DN962067,DN962069,
DN962073,DN962074,DN962077,DN962079-DN962084,DN962086,DN962088,DN962093,DN962096,DN962099,
DN962100,DN962104,DN962105,DN962107,DN962114,DN962121,DN962122,DN962124,DN962126,
DN962133-DN962135,DN962139,DN962141-DN962143,DN962146,DN962147,DN962152,DN962153,
DN962156-DN962158,DN962165,DN962166,DN962171,DN962173,DN962175,DN962177,DN962178,DN962192,
DN962194,DN962196,DN962197,DN962201,DN962203,DN962207,DN962208,DN962211,DN962214,
DN962215,DN962222-DN962224,DN962228,DN962231,DN962233,DN962236,DN962241,DN962244,DN962247,
DN962252,DN962258,DN962264,DN962268-DN962271,DN962276-DN962289,DN962293-DN962298,
DN962301-DN962304,DN962307,DN962311,DN962312,DN962315,DN962318,DN962319,DN962324,DN962328,
DN962331,DN962334,DN962337,DN962339,DN962347,DN962350,DN962354-DN962356,DN962359,
DN962364-DN962366,DN962368,DN962370,DN962372,DN962373,DN962375,DN962380-DN962382,DN962384,
DN962386,DN962389,DN962391,DN962393,DN962395,DN962396,DN962398,DN962400,DN962401,
DN962403,DN962406-DN962408,DN962412-DN962415,DN962418,DN962423,DN962426,DN962432,
DN962435-DN962437,DN962440,DN962441,DN962445,DN962447-DN962453,DN962457-DN962459,
DN962463-DN962465,DN962467,DN962474,DN962476-DN962479,DN962482-DN962484,DN962492,DN962494,
DN962498-DN962502,DN962504,DN962505,DN962507,DN962510,DN962512,DN962513,DN962517,DN962518,
DN962520-DN962523,DN962527,DN962528,DN962533-DN962535,DN962537-DN962542,DN962545,DN962548,
DN962549,DN962554,DN962557,DN962562,DN962563,DN962569,DN962572-DN962576,DN962578-DN962580,
DN962582,DN962585,DN962586,DN962589,DN962591,DN962597-DN962602,DN962605,DN962606,DN962609,
DN962610,DN962612,DN962614-DN962617,DN962619,DN962624-DN962626,DN962631,DN962632,DN962636,
DN962644-DN962647,DN962650,DN962652,DN962653,DN962655,DN962657-DN962660,DN962663,DN962664,
DN962668,DN962669,DN962671,DN962673,DN962674,DN962677,DN962679-DN962682,DN962684,DN962686,
DN962687,DN962690,DN962692-DN962694,DN962699,DN962700,DN962703,DN962704,DN962707,DN962708,
DN962711,DN962712,DN962715,DN962718,DN962722,DN962723,DN962725,DN962728,DN962731,
DN962732,DN962735,DN962736,DN962739,DN962744-DN962749,DN962756,DN962758,DN962759,DN962761,
DN962762,DN962766,DN962770,DN962776,DN962779,DN962781,DN962783,DN962787,DN962788,
DN962790,DN962792,DN962793,DN962801,DN962803,DN962809,DN962811,DN962814,DN962815,
DN962818-DN962822,DN962830,DN962831,DN962832,DN962834,DN962837,DN962840,DN962841,DN962843,
DN962844,DN962852,DN962854,DN962857-DN962862,DN962864,DN962865,DN962867,DN962869,
DN962870-DN962877,DN962879-DN962882,DN962884,DN962886,DN962888,DN962893,DN962895,
DN962897-DN962900,DN962904,DN962906,DN962909,DN962911,DN962917,DN962922,DN962923,DN962925,
DN962927,DN962930-DN962933,DN962935,DN962936,DN962938,DN962939,DN962941,DN962942,DN962945,
-DN962948,DN962950,DN962952,DN962956,DN962957,DN962962,DN962968,DN962979,DN962981,
DN962982,DN962984,DN962985,DN962987-DN962989,DN962991,DN962992,DN962994,DN962995,DN962997,
DN962998,DN963001-DN963015,DN963021,DN963022,DN963025,DN963027,DN963028,DN963032,DN963035,
DN963037,DN963045,DN963046,DN963050,DN963052,DN963055,DN963056,DN963060,DN963064,
DN963065,DN963069,DN963073,DN963080,DN963085,DN963088,DN963090,DN963092-DN963095,
DN963100-DN963103,DN963111,DN963113,DN963114,DN963117,DN963119,DN963120,DN963128,DN963132,
DN963138,DN963142,DN963144,DN963147,DN963151,DN963156,DN963160,DN963161,DN963164,
DN963167,DN963175,DN963179,DN963180,DN963182,DN963185,DN963190,DN963193,DN963197,
DN963203,DN963213-DN963215,DN963217,DN963225,DN963227,DN963228,DN963233,DN963234,DN963237,
DN963240,DN963244,DN963249,DN963260,DN963266,DN963267,DN963270-DN963272,DN963278-DN963282,
DN963284-DN963286,DN963290,DN963291,DN963294,DN963296,DN963298,DN963299,DN963301,DN963308,
DN963311,DN963316,DN963317,DN963319,DN963325,DN963327-DN963329,DN963331,DN963333-DN963335,
DN963339-DN963341,DN963343,DN963350,DN963355,DN963357-DN963359,DN963361,DN963362,DN963367,
DN963373-DN963377,DN963379,DN963383,DN963386,DN963387,DN963388,DN963394-DN963396,DN963399,
DN963401,DN963403,DN963404,DN963406,DN963407,DN963411,DN963414,DN963421,DN963429,
DN963431,DN963434,DN963439-DN963442,DN963449,DN963461,DN963464,DN963465,DN963467,DN963477,
DN963479-DN963483,DN963490,DN963492,DN963493,DN963497,DN963504,DN963507, DN963508,
DN963510-DN963515,DN963521,DN963523,DN963524,DN963529,DN963536,DN963538,DN963539,DN963542,
DN963548-DN963550,DN963552,DN963555-DN963558,DN963561,DN963563,DN963565,DN963568,DN963571,
DN963578,DN963580,DN963581,DN963584,DN963589,DN963593,DN963595,DN963599,DN963600,
DN963602,DN963608,DN963615,DN963620-DN963623,DN963632,DN963633,DN963636,DN963637,
DN963644-DN963648,DN963650-DN963652,DN963655,DN963657,DN963658,DN963660,DN963663,DN963667,
DN963672,DN963674,DN963676,DN963683,DN963685-DN963690,DN963692,DN963696,DN963699,DN963700,
DN963702,DN963703,DN963712,DN963715,DN963720,DN963721,DN963724-DN963726,DN963729,DN963731,
DN963733,DN963735,DN963736,DN963737,DN963741,DN963743,DN963744,DN963749,DN963750,
DN963755,DN963764,DN963766,DN963774,DN963780,DN963782,DN963784-DN963786,DN963795,DN963799,
DN963800,DN963802,DN963806,DN963808,DN963810,DN963813,DN963821,DN963825,DN963826,
DN963830-DN963832,DN963835,DN963838,DN963839,DN963842,DN963844-DN963846,DN963852,DN963853,
DN963856-DN963858,DN963860,DN963863-DN963866,DN963873,DN963877,DN963879,DN963881,DN963888,
DN963889,DN963891,DN963895,DN963898,DN963899,DN963903,DN963905,DN963913,DN963917,
DN963920,DN963921,DN963923,DN963929,DN963932,DN963935-DN963940,DN963943,DN963945,DN963946,
DN963948,DN963954,DN963955,DN963957,DN963966,DN963970,DN963973,DN963977,DN963978,
DN963988,DN963989,DN963993,DN963999,DN964000,DN964003,DN964004,DN964006,DN964013,
DN964015,DN964017,DN964019,DN964024,DN964027,DN964031,DN964033,DN964036,DN964038,
DN964043,DN964048-DN964052,DN964057-DN964062,DN964068,DN964071,DN964073,DN964078,DN964080,
DN964081,DN964083,DN964089,DN964092,DN964093,DN964098-DN964100,DN964103,DN964108,DN964109,
DN964112,DN964115,DN964118,DN964119,DN964125,DN964130-DN964133,DN964136,DN964144-DN964146,
DN964150,DN964154,DN964157,DN964158,DN964162,DN964164,DN964168,DN964173,DN964175,
DN964178,DN964185,DN964187,DN964189,DN964194,DN964195,DN964197,DN964201,DN964202,
DN964205,DN964206,DN964212,DN964214,DN964217,DN964220-DN964224,DN964230,DN964232,DN964234,
DN964235,DN964240,DN964242,DN964243,DN964247,DN964254,DN964256,DN964259,DN964261,
DN964267,DN964271,DN964272,DN964277,DN964278,DN964285,DN964286,DN964288,DN964292,
DN964295,DN964298,DN964300,DN964303,DN964305,DN964306,DN964308,DN964310,DN964320,
DN964334,DN964340,DN964345,DN964347,DN964348,DN964352-DN964355,DN964360,DN964361,DN964362,
DN964364,DN964367,DN964372,DN964374,DN964376,DN964378,DN964386-DN964392,DN964394,DN964396,
DN964402,DN964404,DN964406,DN964409-DN964416,DN964419,DN964420,DN964422,DN964426,
DN964430-DN964436,DN964442,DN964450,DN964452,DN964455,DN964456,DN964462,DN964464,DN964466,
DN964469,DN964472,DN964473,DN964478,DN964481,DN964484-DN964488,DN964492-DN964495,DN964498,
DN964502-DN964504,DN964507,DN964509,DN964512,DN964514-DN964516,DN964518,DN964521-DN964524,
DN964527-DN964529,DN964531,DN964532,DN964534,DN964536,DN964537,DN964538,DN964539,DN964543,
DN964544,DN964547,DN964550,DN964551,DN964552,DN964554,DN964559,DN964560,DN964562,
DN964563-DN964571,DN964574,DN964576,DN964578,DN964580,DN964582,DN964585-DN964594,
DN964598-DN964602,DN964609-DN964612,DN964614-DN964619,DN964622,DN964623,DN964627,DN964628,
DN964630,DN964631,DN964633,DN964635,DN964638,DN964640,DN964642,DN964648,DN964651,
DN964654,DN964656,DN964658-DN964662,DN964664,DN964666,DN964667,DN964669-DN964675,DN964681,
DN964682-DN964684,DN964688,DN964690,DN964693,DN964695-DN964697,DN964699,DN964701,DN964703,
DN964709,DN964712,DN964719,DN964722,DN964723,DN964729,DN964732,DN964733,DN964738-DN964742,
DN964745,DN964747,DN964749,DN964751,DN964752,DN964756,DN964757,DN964759,DN964760,
DN964763,DN964770,DN964771,DN964774-DN964777,DN964781,DN964783-DN964787,DN964791,DN964796,
DN964797,DN964800,DN964804,DN964805,DN964807,DN964809-DN964813,DN964816,DN964820-DN964827,
DN964831-DN964833,DN964835,DN964837-DN964840,DN964844,DN964849-DN964851,DN964854-DN964856,
DN964858,DN964860,DN964862,DN964865,DN964868,DN964870,DN964874,DN964882,DN964885,
DN964886,DN964888,DN964889,DN964896,DN964901,DN964903,DN964910-DN964912,DN964916,DN964919,
DN964921,DN964926,DN964927,DN964930,DN964932,DN964936,DN964938,DN964939,DN964943,
DN964944,DN964947,DN964948,DN964950,DN964953,DN964954,DN964957,DN964959,DN964962,
DN964967,DN964972,DN964973,DN964975,DN964979,DN964984,DN964985,DN964994,DN964996,
DN965001,DN965004,DN965009,DN965013,DN965017,DN965018-DN965021,DN965029,DN965031,DN965043,
DN965055,DN965056,DN965061,DN965063,DN965065-DN965070,DN965080,DN965092,DN965096-DN965099,
DN965102,DN965105,DN965108,DN965111,DN965113,DN965119,DN965121,DN965126-DN965128,DN965131,
DN965133-DN965135,DN965137,DN965140,DN965142,DN965144,DN965147-DN965152,DN965154,DN965157,
DN965158,DN965160,DN965165,DN965168,DN965175,DN965182,DN965183,DN965185,DN965194,
DN965197,DN965198,DN965207,DN965212,DN965213,DN965220,DN965224,DN965236,DN965244,
DN965245,DN965258,DN965267,DN965269-DN965272,DN965275-DN965278,DN965280,DN965282,DN965284,
DN965290,DN965291,DN965293,DN965294,DN965298,DN965301,DN965307,DN965308,DN965313,
DN965315,DN965321,DN965323,DN965327,DN965331,DN965335,DN965337,DN965339-DN965343,DN965348,
DN965352,DN965353,DN965357-DN965359,DN965368,DN965371,DN965373-DN965377,DN965382,DN965388,
DN965390,DN965392,DN965394-DN965396,DN965398,DN965400,DN965402,DN965404,DN965405,
DN965410-DN965412,DN965414,DN965421,DN965423-DN965425,DN965428,DN965431-DN965433,DN965435,
DN965436,DN965439-DN965445,DN965447,DN965448,DN965450-DN965452,DN965454-DN965456,DN965459,
DN965461,DN965463,DN965465,DN965468-DN965470,DN965473,DN965476-DN965485,DN965487-DN965495,
DN965497,DN965498,DN965500,DN965503,DN965506,DN965511,DN965513,DN965515,DN965517-DN965519,
DN965522,DN965523,DN965525,DN965528,DN965530,DN965532,DN965535,DN965538-DN965540,DN965542,
DN965546-DN965551,DN965558,DN965559,DN965561,DN965563,DN965564,DN965568,DN965572,DN965578,
DN965579,DN965581-DN965583,DN965595,DN965596,DN965598,DN965600,DN965601,DN965603,DN965604,
DN965606,DN965608,DN965609,DN965611,DN965613,DN965621,DN965623,DN965627,DN965632,
DN965633,DN965637,DN965647,DN965654,DN965658,DN965660,DN965661,DN965663,DN965665,
DN965674,DN965676,DN965679,DN965681,DN965684,DN965686,DN965690,DN965691,DN965694,
DN965695,DN965697,DN965699,DN965702,DN965704,DN965706,DN965707,DN965709,DN965719,
DN965721,DN965735,DN965736,DN965737,DN965742,DN965743,DN965749-DN965751,DN965760,DN965767,
DN965769,DN965774,DN965776,DN965777,DN965786,DN965790,DN965791,DN965793,DN965796,
DN965801,DN965804,DN965807-DN965812,DN965815,DN965821,DN965831,DN965833,DN965835,DN965837,
DN965838,DN965847,DN965848,DN965850,DN965853,DN965855,DN965858,DN965860,DN965864,
DN965866,DN965867,DN965871,DN965873,DN965875,DN965879,DN965882,DN965883,DN965886,
DN965889,DN965895-DN965897,DN965900,DN965901,DN965903,DN965905,DN965908,DN965909,DN965914,
DN965915,DN965918,DN965919-DN965925,DN965934-DN965938,DN965942,DN965946,DN965959,DN965962,
DN965964,DN965966,DN965970,DN965971,DN965975-DN965977,DN965979,DN965980,DN965984,DN965985,
DN965987,DN965988,DN965997-DN965999,DN966006,DN966010,DN966013,DN966017,DN966019,DN966021,
DN966023,DN966025,DN966031,DN966033,DN966035,DN966041,DN966050,DN966052,DN966054,
DN966057,DN966058,DN966064,DN966066,DN966072,DN966073,DN966075,DN966088,DN966089,
DN966102,DN966112,DN966116,DN966121,DN966122,DN966127,DN966133,DN966135,DN966140,
DN966142,DN966147,DN966157,DN966159,DN966160,DN966161,DN966164,DN966166,DN966170,
DN966177,DN966179,DN966180,DN966184,DN966186,DN966191,DN966196,DN966201,DN966203,
DN966206,DN966208,DN966211,DN966213,DN966214,DN966216,DN966217,DN966221,DN966223,
DN966228,DN966231,DN966233,DN966241,DN966244,DN966250,DN966251,DN966257,DN966259,
DN966260,DN966268,DN966272,DN966286,DN966289,DN96629 1,DN966293,DN966295,DN966297,
DN966300,DN966301,DN966309,DN966315,DN966325,DN966330,DN966331-DN966356,DN966358,DN966364,
DN966365,DN966367,DN966370,DN966380,DN966383,DN966395,DN966400,DN966405,DN966406,
DN966410,DN966414-DN966417,DN966424,DN966427,DN966436,DN966438,DN966451,DN966453,DN966464,
DN966467,DN966468,DN966470,DN966472,DN966475,DN966491,DN966496,DN966498,DN966501,
DN966504,DN966505,DN966512,DN966513,DN966524,DN966528,DN966530,DN966537,DN966538,
DN966540,DN966543,DN966550,DN966552,DN966553,DN966555,DN966556,DN966558,DN966562,
DN966566-DN966568,DN966573-DN966576,DN966579,DN966587,DN966591,DN966593,DN966596,DN966599;
3)利用通用引物PCR扩增目的片段,纯化后的探针浓度应大于100ng/μl;所述通用引物序列为序列表中的SEQ ID №:222和SEQ ID №:223;
4)将步骤3)纯化后的探针干燥后溶于50%DMSO中,点样溶液中的探针浓度大于100ng/μl;
5)点样:将步骤4)的探针溶液点在载玻片上,点制在载玻片上的样品还包括若干个与已知基因没有相似性的基因间序列作为外标,干燥后得到用于检测十字花科植物在胁迫条件下基因表达信息的cDNA芯片。
3、根据权利要求2所述的制备方法,其特征在于:所述步骤3)中制备的探针还包括若干个软腐欧文氏细菌的致病基因片段作为阴性对照。
4、根据权利要求3所述的制备方法,其特征在于:所述软腐欧文氏细菌的致病基因片段为具有下述GenBank登录号的DNA片段:AF022772、M36651.1、AF025769.2、L78834.1。
5、根据权利要求2所述的制备方法,其特征在于:所述步骤5)中每个探针设3个重复。
6、根据权利要求2所述的制备方法,其特征在于:所述步骤4)中的载玻片为经过氨基化学修饰的载玻片。
7、根据权利要求6所述的制备方法,其特征在于:所述载玻片为多聚赖氨酸载玻片。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2005101092942A CN100547079C (zh) | 2005-10-20 | 2005-10-20 | 检测十字花科植物在胁迫条件下基因表达信息的cDNA芯片 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2005101092942A CN100547079C (zh) | 2005-10-20 | 2005-10-20 | 检测十字花科植物在胁迫条件下基因表达信息的cDNA芯片 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1952172A true CN1952172A (zh) | 2007-04-25 |
CN100547079C CN100547079C (zh) | 2009-10-07 |
Family
ID=38058728
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB2005101092942A Expired - Fee Related CN100547079C (zh) | 2005-10-20 | 2005-10-20 | 检测十字花科植物在胁迫条件下基因表达信息的cDNA芯片 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN100547079C (zh) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101096706B (zh) * | 2007-06-29 | 2012-03-28 | 扬州大学 | 检测apec、upec毒力基因表达水平的dna芯片及其构建方法 |
CN108531548A (zh) * | 2018-04-09 | 2018-09-14 | 上海芯超生物科技有限公司 | 一种细胞cDNA芯片及其制备方法和应用 |
CN112795570A (zh) * | 2019-11-12 | 2021-05-14 | 江苏师范大学 | 拟南芥转录因子at5g59820基因在培育抗病性转基因植物中的应用 |
CN113980987A (zh) * | 2021-12-06 | 2022-01-28 | 上海市农业科学院 | 一种提高植物抗镍性能的PgIREG1S和AlATP-PRTS双基因组及其应用 |
-
2005
- 2005-10-20 CN CNB2005101092942A patent/CN100547079C/zh not_active Expired - Fee Related
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101096706B (zh) * | 2007-06-29 | 2012-03-28 | 扬州大学 | 检测apec、upec毒力基因表达水平的dna芯片及其构建方法 |
CN108531548A (zh) * | 2018-04-09 | 2018-09-14 | 上海芯超生物科技有限公司 | 一种细胞cDNA芯片及其制备方法和应用 |
CN112795570A (zh) * | 2019-11-12 | 2021-05-14 | 江苏师范大学 | 拟南芥转录因子at5g59820基因在培育抗病性转基因植物中的应用 |
CN112795570B (zh) * | 2019-11-12 | 2022-06-28 | 江苏师范大学 | 拟南芥转录因子at5g59820基因在培育抗病性转基因植物中的应用 |
CN113980987A (zh) * | 2021-12-06 | 2022-01-28 | 上海市农业科学院 | 一种提高植物抗镍性能的PgIREG1S和AlATP-PRTS双基因组及其应用 |
Also Published As
Publication number | Publication date |
---|---|
CN100547079C (zh) | 2009-10-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US7906286B2 (en) | Probe set, probe carrier, and method for determining and identifying fungus | |
US6376191B1 (en) | Microarray-based analysis of polynucleotide sequence variations | |
EP1759011B1 (en) | Detection of chromosomal disorders | |
KR20020008190A (ko) | 마이크로어레이에 기준한 삭감식 혼성화 | |
CN111286530A (zh) | 一种基于核酸质谱法检测27种呼吸道病原体的引物组、试剂盒及其应用 | |
EP1969145B1 (en) | Oligonucleotide microarray and method for identification of pathogens | |
CN100547079C (zh) | 检测十字花科植物在胁迫条件下基因表达信息的cDNA芯片 | |
CN114774409A (zh) | 基于224个InDel和57个SNP位点的二代测序检测体系 | |
CN111961763A (zh) | 一种新型冠状病毒检测基因芯片 | |
KR100666099B1 (ko) | K―ras 올리고뉴클레오티드 마이크로어레이 및 이를이용하여 K―ras 유전자의 돌연변이를 검사하는 방법 | |
CN114277033B (zh) | 中国桔梗rpl13内参基因序列及其引物与应用 | |
CN110241212A (zh) | 一种用于brca1和brca2基因扩增子测序检测的引物组及其应用 | |
KR100611056B1 (ko) | 병원성 미생물 검출을 위한 올리고뉴클레오티드마이크로칩 및 이를 이용한 병원성 미생물 검출방법 | |
US20090136916A1 (en) | Methods and microarrays for detecting enteric viruses | |
JPH0690798A (ja) | 黄色ブドウ球菌検出用プローブ及び検出方法 | |
WO2002090516A2 (en) | Design of artificial genes for use as controls in gene expression analysis systems | |
KR102490750B1 (ko) | 수박 품종 식별용 snp 마커 및 이의 이용 | |
KR101858962B1 (ko) | 단일염기다형성 (snp) 마커를 이용한 감 품종식별 방법 및 키트 | |
KR102672578B1 (ko) | 사초과 식물 '양지사초'를 판별하기 위한 snp 마커 조성물 및 이의 용도 | |
KR102672563B1 (ko) | 사초과 식물 '좀목포사초'를 판별하기 위한 snp 마커 조성물 및 이의 용도 | |
KR102672572B1 (ko) | 사초과 식물 '가는흰사초'를 판별하기 위한 snp 마커 조성물 및 이의 용도 | |
KR102672574B1 (ko) | 사초과 식물 '진도사초'를 판별하기 위한 snp 마커 조성물 및 이의 용도 | |
KR102672571B1 (ko) | 사초과 식물 '가지청사초'를 판별하기 위한 snp 마커 조성물 및 이의 용도 | |
KR102672581B1 (ko) | 사초과 식물 '주름청사초'를 판별하기 위한 snp 마커 조성물 및 이의 용도 | |
KR101774275B1 (ko) | 고추의 여교배 육종을 위한 단일염기다형성 마커 세트 및 이의 용도 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C17 | Cessation of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20091007 Termination date: 20111020 |