CN1951483A - Hybrid safflower oil and preparation method thereof - Google Patents
Hybrid safflower oil and preparation method thereof Download PDFInfo
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- CN1951483A CN1951483A CN 200610114681 CN200610114681A CN1951483A CN 1951483 A CN1951483 A CN 1951483A CN 200610114681 CN200610114681 CN 200610114681 CN 200610114681 A CN200610114681 A CN 200610114681A CN 1951483 A CN1951483 A CN 1951483A
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- safflower oil
- methyl salicylate
- thymol
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Abstract
The invention discloses a safflower oil and process for preparation, wherein the main constituents include methyl salicylate, eucalyptus oil, citronella oil, clove oil, thymol, tea seed oil. The preparing process consists of steps of mixing homogeneously, charging tea seed oil and filtering. The obtained product contains C8H8O3 50.0-61.2% (g/g).
Description
Technical field
The present invention relates to a kind of promoting blood circulation and stopping pain, be used for the treatment of the medicine that lumbago due to pathogenic wind-dampness, iliacus are sprained pain, nameless gall, belly pain due to cold heat.The invention still further relates to the preparation method of this medicine, the invention reside in provides the scientific matching of a kind of the best of the present invention method, and has formulated the discrimination method and the content assaying method of this medicine.
Background technology
Safflower oil is a kind of wind dispelling of invigorating blood circulation, muscles and tendons relaxing to alleviate pain, and the external used medicine commonly used of treatment rheumatic ostalgia, numb limbs and tense tendons, traumatic injury and mosquito bite and insect bite has obtained using widely clinically, and its curative effect is affirmed by extensive patients.
Summary of the invention
The present invention has aimed to provide a kind of safflower oil and preparation method thereof.
The present invention is the medicament made from following raw material: methyl salicylate, Bay leaves oil, citronella oil, Oleum Caryophylli, thymol, tea-seed oil.
Wherein each proportion of raw materials is:
Methyl salicylate 500～600g Bay leaves oil 100～250g
Citronella oil 50～150g Oleum Caryophylli 100～200g
Thymol 20～50g tea-seed oil 5～60g
Optimal proportion of the present invention is:
Methyl salicylate 556g Bay leaves oil 150g
Citronella oil 100g Oleum Caryophylli 150g
Thymol 30g tea-seed oil 14g
The preparation method of safflower oil is:
Take by weighing methyl salicylate, Bay leaves oil, citronella oil, Oleum Caryophylli, thymol, fully mix homogeneously adds tea-seed oil to 1000g and mix homogeneously, filters promptly.The gained safflower oil wherein contains methyl salicylate (C
8H
8O
3) should be: 50.0%～61.2% (g/g).According to the proportioning of above-mentioned raw materials, make liniment, externally used paste, medicated wine, oil preparation, drop pill, smears.
Using method of the present invention: suitable amount used externally is applied to the affected part.
Character of the present invention: rufous clarified oil dress liquid, gas fragrance.
Relative density: in the time of 20 ℃, should be 1.05～1.09 (appendix VIIA of Chinese Pharmacopoeia nineteen ninety-five version).
Index of refraction: in the time of 20 ℃, should be 1.495～1.535 (appendix VIIF of Chinese Pharmacopoeia nineteen ninety-five version).
Assay of the present invention: adopt methyl salicylate to measure according to gas chromatography (appendix VIE of Chinese Pharmacopoeia nineteen ninety-five version);
System suitability test---with PEG-20M is immobile phase, and coating concentration is 10%, is carrier with Chromosorb WAW-DMCS, 160 ℃ of column temperatures, and theoretical cam curve should be not less than 2000 in methyl salicylate, and separating degree should be up to specification;
Need testing solution preparation---it is an amount of to get this product, accurately claims surely, adds dehydrated alcohol and makes the solution that every ml contains 10mg, shakes up, promptly;
The reference substance solution preparation---it is an amount of to get the methyl salicylate reference substance, and accurate the title decides, and adds dehydrated alcohol and makes dissolving, makes the solution that every ml contains 5.0mg, shakes up, promptly;
Algoscopy---precision is measured reference substance solution, each 1ml of need testing solution respectively, and inject gas chromatograph is measured, and calculates, promptly;
This product contains methyl salicylate (C
8H
8O
3) should be 50.0%～61.2% (g/g).
Discrimination method of the present invention:
It is an amount of to get this product, adds dehydrated alcohol, makes the solution that every ml contains 30mg, as need testing solution.Other gets citronella oil, methyl salicylate, cinnamic aldehyde is reference substance, adds dehydrated alcohol and makes it dissolving, makes the solution that every ml contains 3mg, 10mg, 3mg respectively, in contrast product solution.According to the gas chromatography determination in the top described assay, 180 ℃ of column temperatures are drawn above-mentioned three kinds of reference substance solution and each 1ml of need testing solution, and inject gas chromatograph, test sample should present the chromatographic peak identical with the reference substance retention time.
The specific embodiment
The preparation method of safflower oil is:
1, takes by weighing methyl salicylate 556g, Bay leaves oil 150g, citronella oil 100g, Oleum Caryophylli 150g, thymol 30g, tea-seed oil 14g;
2, with the abundant mix homogeneously of methyl salicylate, Bay leaves oil, citronella oil, Oleum Caryophylli, thymol that takes by weighing;
3, will add tea-seed oil in the methyl salicylate of abundant mix homogeneously, Bay leaves oil, citronella oil, Oleum Caryophylli, the thymol to the 1000g mix homogeneously;
4, filtration step 3 products obtained therefroms;
5, step 4 products obtained therefrom is made liniment, externally used paste, medicated wine, oil preparation, drop pill, smears respectively.
Introduce the embodiment of safflower oil pharmacological evaluation of the present invention below.
Embodiment 1 is the safflower oil pharmacological research, with the analgesic effect of mice acetic acid twisting method observation safflower oil, observes the resist inflammation on repercussive function of safflower oil with the scorching method of Mice Auricle caused by dimethylbenzene xylene.
Test material
Test sample: safflower oil of the present invention.
Reference substance: negative control is a normal saline, the laboratory autogamy; Positive reference substance is a WANHUAYOU; End oil contrast is Oleum Camelliae.
Animal: SPF level NIH white mice, SPF level SD kind rat; SPF level animal specific standard particle feedstuff.
Experimental situation: SPF level Animal Lab., 24 ℃～26 ℃ of room temperatures, relative humidity 60%～70% per hour renews wind 15 times.
Experimental technique is selected: mice acetic acid twisting method is adopted in the analgesic test; The scorching method of Mice Auricle caused by dimethylbenzene xylene is selected in the anti-inflammation detumescence test for use.Medication adopts the animal partial smearing.
Test method and result
Acetic acid twisting method: select the 18-22g white mice, ♀ ♂ half and half, be divided into totally 4 groups of safflower oil, WANHUAYOU, Oleum Camelliae and normal saline at random, every group 12, smear each about 0.2ml of safflower oil, WANHUAYOU, Oleum Camelliae and normal saline respectively at each group mouse web portion, respectively organize the equal ip0.6% acetic acid of mice 0.1ml/10g body weight after 1 hour, count behind the ip writhing response number of times of every mice in 15 minutes immediately.Result of the test sees Table 1.
Table 1 safflower oil is to the influence (X ± SD time) of mouse writhing number of times
| Group | Number of animals (only) | Turn round the body number of times | Analgesia rate (%) | P |
| Normal saline | 12 | 52.1±9.8 | ||
| Safflower oil | 12 | 16.8±5.6 | 57.8 | ＜0.01 |
| WANHUAYOU | 12 | 26.9±9.5 | 48.4 | ＜0.01 |
| Oleum Camelliae | 12 | 42.8±11.3 | 17.9 | ＞0.05 |
The result as seen, safflower oil can obviously reduce the mouse writhing reaction times of being brought out by acetic acid, remarkable with normal saline matched group comparing difference, the analgesia rate reaches 67.8%.
The safflower oil resist inflammation on repercussive function
Xylol causes the influence of Mice Auricle inflammation: select the 20-23g white mice for use, ♀ ♂ half and half, be divided into 4 groups the same.Be coated with the about 0.02ml of melted paraxylene for the wide tow sides of every mouse right ear and cause inflammation, left auricle is a normal control.After causing scorching 30 minutes, each is organized mouse right ear and causes scorching position and be coated with and drip a safflower oil, WANHUAYOU, Oleum Camelliae and each about 0.05ml of normal saline.Drew neck to put to death mice in 1 hour behind the coating, cut ears, clean auris dextra oil or moisture with filter paper along the ear baseline, lay homalographic circle auricle with card punch in the ears same area, precision torsion balance claims auricle weight, is the swelling degree with left and right sides auricle weight difference (mg), relatively each group difference.The results are shown in Table 2.
Table 2 safflower oil xylol causes the influence (X ± SDmg) of ear swelling
| Group | Number of animals (only) | The ear swelling degree | P |
| Normal saline | 12 | 6.92±2.30 | |
| Safflower oil | 12 | 4.17±1.60 | ＜0.05 |
| WANHUAYOU | 12 | 4.25±2.39 | ＜0.05 |
| Oleum Camelliae | 12 | 7.00±1.92 | ＞0.05 |
The result shows that Flos Carthami line of oils mice ear degree is starkly lower than the normal saline group, and inhibitory rate of intumesce reaches 39.7%.
Conclusion (of pressure testing)
This conclusion (of pressure testing) shows that the safflower oil external can obviously reduce acetic acid and cause the mouse writhing reaction times, and improving stimulates the mice threshold of pain, reduces the scorching mice auricle swelling degree of caused by dimethylbenzene xylene, shows that external of the present invention has pain relieving and resist inflammation on repercussive function.
Embodiment 2 is that safflower oil is to skin irritation and sensitivity test
1. skin irritation test
Test objective
Observe the local excitation reaction and the extent of reaction that are produced behind animal intact skin and the damaged skin contact safflower oil.
Test material
Animal: regular grade adult healthy albino guinea-pig, about body weight 0.25kg, ♀ ♂ half and half.
Test sample: safflower oil of the present invention.
Reference substance: Oleum Camelliae.
Depilatory: sodium sulfide, soap powder, starch.
Test method: get 12 of healthy albino guinea-pigs, be divided into 2 groups, be i.e. intact skin group and damaged skin group.Two treated animals with depilatory with spinal column both sides skin depilatory after second day, do not see skin injury or irritant reaction.Depilation district, damaged skin group both sides makes # stroke trace with injection needle behind 75% alcohol disinfecting, the degree of being with the oozing of blood, the about 2 * 2cm of area.Test is adopted from body left and right sides own control, and two treated animals depilation district drips with safflower oil 0.2ml in the left side that (scraps of paper 2.5 * 2.5cm) are affixed on skin surface, cover oilpaper and gauze, immobilization with adhesive tape on 2 floor sterilized filter paper sheets; The right side is used with the quadrat method administration with Oleum Camelliae.Remove medicine after 5 hours,, observe 1 hour, 24 hours, 48 hours and the 72 hours dermoreaction situations behind the test sample removed then, mark and estimate stimulus intensity according to table 3 with warm water flush away left drug.
Table 3 skin irritation reaction standards of grading
| Erythema | Score value | Edema | Score value |
| No erythema | 0 | No edema | 0 |
| Reluctantly as seen | 1 | Reluctantly as seen | 1 |
| Obvious erythema | 2 | The cutaneous protuberance profile is clear | 2 |
| Moderate is to serious erythema | 3 | Edema protuberance 1mm also enlarges | 3 |
| Purple erythema and eschar | 4 |
Result of the test (referring to table 4): the intact skin group is observed to 72 hours after giving safflower oil, and animal intact skin part is show speckle or edema not; The damaged skin group is given visible erythema behind the safflower oil, and the rear section animal still had erythema in 24 hours, and only reluctantly as seen, show speckle not after 72 hours there is no edema after 48 hours.
Table 4 safflower oil is to skin irritation average response value
| Group | Number of animals (only) | The integration summation | The average response value | ||||||
| 1h | 24h | 48h | 72h | 1h | 24h | 48h | 72h | ||
| The normal skin group | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| The damaged skin group | 6 | 12 | 8 | 3 | 0 | 2 | 1.3 | 0.5 | 0 |
2. skin allergy test
Test objective
Observe animal skin and repeat to contact behind the safflower oil body immune system in the reaction of skin.
Test material
All test with skin irritation; Positive reference substance is with 2, the 4-dinitrochlorobenzene.
Test method
Select healthy albino guinea-pig, about body weight 250kg, be divided into Flos Carthami line of oils, Oleum Camelliae group, positive controls by the sex body weight, 8 every group, ♀ ♂ half and half.Each treated animal is tested left side depilation earlier in preceding 24 hours, and (used and skin irritation same procedure administration 0.15ml in the depilation district in about the 3 * 3cm), 2nd day, positive controls gives 1%2, and 4-dinitrochlorobenzene 0.15ml removed test sample after 6 hours.Give test sample 1 time, totally 3 times every other day with quadrat method.Last is to 14 days (it is the same with the right side skin depilatory to carry the previous day) behind the test sample, with excite administration (it is 0.1% that positive control drug excites concentration) in right side depilation district with quadrat method, remove test sample after 6 hours, at once observe, observed the skin allergy situation then once more in 24 hours, 48 hours and 72 hours, according to scoring of table 5 standard and evaluation sensitization degree, calculate the sensitization rate.
Table 5 skin allergy standards of grading
| Erythema | Score value | Edema | Score value |
| No erythema | 0 | No edema | 0 |
| Slight erythema | 1 | Mild edema | 1 |
| Moderate erythema | 2 | Intermediate edema | 2 |
| The severe erythema | 3 | The severe edema | 3 |
| Edematous erythema 4 | 4 |
Result of the test is referring to table 6.
Table 6 safflower oil is to the guinea pig skin anaphylaxis
| Group | Number of animals (only) | The average response value | Sensitization rate % | ||
| 24h | 48h | 72h | |||
| The Flos Carthami line of oils | 8 | 0 | 0 | 0 | 0 |
| The Oleum Camelliae group | 8 | 0 | 0 | 0 | 0 |
| Positive controls | 8 | 3 | 3 | 1.5 | 100 |
The result as seen, the positive controls Cavia porcellus excites back 24 hours, 48 hours, and moderate erythema and Mild edema are all arranged, and still has 6/8 degree of taking a favourable turn edema and slight erythema after 72 hours, the sensitization rate is 100%; There is no erythema and edema after Flos Carthami line of oils and the self-excitation of Oleum Camelliae group to 72 hours.
Conclusion (of pressure testing)
Test shows that safflower oil has minimal irritation to the normal skin nonirritant to damaged skin, and skin is not had sensitization.
The above embodiment only is the preferred embodiments of the present invention; the invention is not restricted to the foregoing description; for persons skilled in the art; the any conspicuous change of under the prerequisite that does not deviate from the principle of the invention it being done all belongs to the protection domain of design of the present invention and claims.
Claims (5)
1. a safflower oil is characterized in that it is the medicament made from following raw material: methyl salicylate, Bay leaves oil, citronella oil, Oleum Caryophylli, thymol, tea-seed oil.
2. safflower oil as claimed in claim 1 is characterized in that described each proportion of raw materials is:
Methyl salicylate 500～600g Bay leaves oil 100～250g
Citronella oil 50～150g Oleum Caryophylli 100～200g
Thymol 20～50g tea-seed oil 5～60g
3. safflower oil as claimed in claim 1 or 2 is characterized in that wherein each proportion of raw materials is:
Methyl salicylate 556g Bay leaves oil 150g
Citronella oil 100g Oleum Caryophylli 150g
Thymol 30g tea-seed oil 14g
4. safflower oil as claimed in claim 1 is characterized in that described medicament is: liniment, externally used paste, medicated wine, oil preparation, drop pill, smears.
5. as the preparation method of the described safflower oil of one of claim 1 to 5, it is characterized in that: take by weighing methyl salicylate, Bay leaves oil, citronella oil, Oleum Caryophylli, thymol, fully mix homogeneously adds tea-seed oil to 1000g and mix homogeneously, filters promptly.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610114681A CN1951483B (en) | 2006-11-21 | 2006-11-21 | Hybrid safflower oil and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610114681A CN1951483B (en) | 2006-11-21 | 2006-11-21 | Hybrid safflower oil and preparation method thereof |
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| CN1951483A true CN1951483A (en) | 2007-04-25 |
| CN1951483B CN1951483B (en) | 2010-05-26 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102151309A (en) * | 2010-02-11 | 2011-08-17 | 张金荣 | External preparation with functions of dispelling stasis and relieving pain, preparation method and detection method thereof |
| CN102151311A (en) * | 2010-02-11 | 2011-08-17 | 张金荣 | External preparation with functions of removing obstruction in channels and relieving pain, preparation method and detection method thereof |
-
2006
- 2006-11-21 CN CN200610114681A patent/CN1951483B/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102151309A (en) * | 2010-02-11 | 2011-08-17 | 张金荣 | External preparation with functions of dispelling stasis and relieving pain, preparation method and detection method thereof |
| CN102151311A (en) * | 2010-02-11 | 2011-08-17 | 张金荣 | External preparation with functions of removing obstruction in channels and relieving pain, preparation method and detection method thereof |
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| Publication number | Publication date |
|---|---|
| CN1951483B (en) | 2010-05-26 |
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Granted publication date: 20100526 Termination date: 20201121 |