CN102151311A - External preparation with functions of removing obstruction in channels and relieving pain, preparation method and detection method thereof - Google Patents
External preparation with functions of removing obstruction in channels and relieving pain, preparation method and detection method thereof Download PDFInfo
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- CN102151311A CN102151311A CN2010101118290A CN201010111829A CN102151311A CN 102151311 A CN102151311 A CN 102151311A CN 2010101118290 A CN2010101118290 A CN 2010101118290A CN 201010111829 A CN201010111829 A CN 201010111829A CN 102151311 A CN102151311 A CN 102151311A
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Abstract
The invention relates to an external preparation with functions of removing obstruction in channels and relieving pain, a preparation method and a detection method thereof, belonging to the technical field of pharmacy. The external preparation is prepared from raw materials such as cinnamon leaf oil, cinnamaldehyde and the like, and has a better effect. The preparation has good stability, is convenient to use, and is easy to accept by patients. The preparation method provided by the invention can be used for effectively preparing the required preparation and ensuring that the obtained preparation is scientific and reasonable in the production process. The detection method provided by the invention effectively ensures preparation quality.
Description
Technical field
The present invention is external preparation of a kind of removing obstruction in the collateral to relieve pain and preparation method thereof and detection method, belongs to technical field of pharmacy.
Technical background
Muscles and bones aching is puzzlement people's commonly encountered diseases, and be how relevant with daily life system, dietary habit, working environment and sport injury.Symptom comprises that muscular soreness, cervical region are ached, elbow wrist pain, shoulder are ached, sore waist and aching in the waist and the back, knee endoprosthesis pain, bony spur pain etc.Young patient, common elbow wrist pain, muscle neck pain result from and do not note the life and work details, and for example long-time operation computer, neck muscle can't loosen, the resistance of the cervical region blood circulation stasis of blood.Cervical region, waist and arthralgia that middle age takes place are primary symptom with muscular fasciae syndrome, cervical nerves pain, bony spur pain, gout, bursitis of shoulder; The old people is then maximum to suffer from osteoarthritis.
In view of such circumstances, providing a kind of determined curative effect, toxic and side effects product little, easy to use is the thing of being badly in need of solution.
Summary of the invention
Technical problem to be solved by this invention provides external preparation of a kind of removing obstruction in the collateral to relieve pain and preparation method thereof and detection method; The present invention is directed to prior art, the product determined curative effect, the toxic and side effects that provide are little, easy to use.
Technical scheme of the present invention is to constitute like this: it is made up of following raw materials according: Bay leaves oil 2-6 part, cinnamic aldehyde 3-7 part, citronella oil 1-3 part, Oleum Terebinthinae 18-28 part, wintergreen oil 60-75 part.Be preferably: 4 parts of Bay leaves oils, 5 parts of cinnamic aldehyde, 2 parts of citronella oil, 22 parts of Oleum Terebinthinaes, 67 parts of wintergreen oils.
The preparation method of described external preparation: the raw material of getting it filled, make multiple exterior-applied formulation respectively, comprising: medicine oil, unguentum, gel, lotion, spray, patch, tincture.Elect as: take by weighing Bay leaves oil, cinnamic aldehyde, citronella oil, Oleum Terebinthinae and stir, add the wintergreen oil dilution, promptly get medicine oil.
The discrimination method of described external preparation comprises following all or part of content: (1) adopts gas chromatography, contrasts with australene, methyl salicylate reference substance, and this product shows the chromatographic peak identical with the reference substance retention time; (2) adopt thin layer chromatography, contrast, on this product and the corresponding position of reference substance chromatograph, show the speckle of same color with the cinnamic aldehyde reference substance.Be preferably: discrimination method comprises following all or part of content:
(1) it is an amount of to get this product, adds anhydrous alcohol solution, makes need testing solution; Get australene, the methyl salicylate reference substance is an amount of, add dehydrated alcohol and make reference substance solution; Gas chromatography determination, test sample chromatograph should present the chromatographic peak identical with the reference substance chromatographic retention;
(2) it is an amount of to get this product, adds anhydrous alcohol solution, makes need testing solution; It is an amount of that other gets the cinnamic aldehyde reference substance, adds anhydrous alcohol solution, makes reference substance solution; According to the thin layer chromatography test, draw above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, with petroleum ether: ethyl ester acetic acid=17: 3 is developing solvent, launch, take out, dry, spray is with the dinitrophenylhydrazine alcoholic solution, 105 ℃ were dried by the fire 5 minutes, and inspected under the daylight, in the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.
The assay employing gas chromatography determination australene of described external preparation is rare, the content of methyl salicylate.Be preferably: take by weighing respectively that australene is rare, the methyl salicylate reference substance is an amount of, add dehydrated alcohol and make reference substance solution; Get this product, the accurate title, decide, and puts in the measuring bottle, adds the dehydrated alcohol dilution, as need testing solution; Precision is measured reference substance solution and each 1 μ l of need testing solution respectively, and inject gas chromatograph is measured, calculated weight percentage ratio, and this product contains australene should be lower than 16.5%, contains methyl salicylate and should be 60.3-73.7%.
We are made up of Bay leaves oil, cinnamic aldehyde, citronella oil, Oleum Terebinthinae, wintergreen oil, have the effect of dispelling wind and removing obstruction in the collateral, dispersing cold for relieving pain.Be used for muscles and bones aching, traumatic injury, muscle fatigue.
Experimental example
1 group: 2 parts of Bay leaves oils, 3 parts of cinnamic aldehyde, 1 part of citronella oil, 18 parts of Oleum Terebinthinaes, 60 parts of wintergreen oils
2 groups: 6 parts of Bay leaves oils, 7 parts of cinnamic aldehyde, 3 parts of citronella oil, 28 parts of Oleum Terebinthinaes, 75 parts of wintergreen oils
3 groups: 4 parts of Bay leaves oils, 5 parts of cinnamic aldehyde, 2 parts of citronella oil, 22 parts of Oleum Terebinthinaes, 67 parts of wintergreen oils
1, analgesic test (writhing method)
Mice is divided into 4 groups at random, 10 every group.Be matched group and 3 product treatment groups.4 groups of mice 24h abdominal part depilations before administration, area is 1.5cm * 1.5cm, requires no skin injury.The treatment group is applied to depilation place respectively with 3 group of products and covers with one deck oilpaper, covers monolayer gauze parcel again, uses immobilization with adhesive tape then; Matched group is coated with vaseline in depilation place, handles the same.Continuous external application 7d, in the 8th day lumbar injection 0.7% glacial acetic acid solution 0.1ml/10g body weight, each mouse writhing number of times in the 20min behind observation and the record injection algogen.
Group is turned round the body number of times
Matched group 50.2 ± 3.45
1 group 43.1 ± 1.36
2 group 42.3 ± 2.89
3 group 39.2 ± 7.11
The result shows that preparation of the present invention has good analgesic activity.
2, to the influence of mice blood stasis speckle score value
Get 40 male mices, be divided into 4 groups at random, 10 every group, with every Mus abdominal part depilation 2 * 2cm, 24h is in abdominal part depilation district
SC0.05ml the mouse anti coagulableblood forms the circular blood mound of swelling, immediately the abdomen coating medicine-feeding.The blank group is given 0.9% sodium chloride solution, is subjected to the reagent group to organize for this product 1-3, and every day, liniment was 2 times, each 1h.12h, 24h, 48h difference observed and recorded ecchymosis size, the order of severity behind the injection anticoagulated blood.Press ecchymosis size, order of severity evaluation criteria score value, its score value standards of grading are: no ecchymosis is 0 grade; The ecchymosis that slightly mays be seen indistinctly is 0.5 grade; Slight ecchymosis is 1 grade; The moderate ecchymosis is 2 grades; The severe ecchymosis is 3 grades; The blood mound is obvious, and the serious hyperemia of blood stasis speckle is 4 grades.
Group ecchymosis score value
12h 24h 48h
Blank group 3.6 ± 0.17 3.3 ± 0.29 3.0 ± 0.23
1 group 1.6 ± 0.21 1.3 ± 0.05 1.0 ± 0.07
2 group 1.7 ± 0.28 1.5 ± 0.03 1.2 ± 0.61
3 group 1.4 ± 0.98 1.2 ± 0.18 0.9 ± 0.25
The result: this product is 12h to 48h behind coating, and blood stasis speckle score value is obviously reduced.
Concrete embodiment
Embodiment 1: 4 parts of Bay leaves oils, 5 parts of cinnamic aldehyde, 2 parts of citronella oil, 22 parts of Oleum Terebinthinaes, 67 parts of wintergreen oils
Take by weighing Bay leaves oil, cinnamic aldehyde, citronella oil, Oleum Terebinthinae and stir, add the wintergreen oil dilution, promptly get medicine oil.External is applied to the affected part.
Embodiment 2: 2 parts of Bay leaves oils, 3 parts of cinnamic aldehyde, 1 part of citronella oil, 18 parts of Oleum Terebinthinaes, 60 parts of wintergreen oils
Taking by weighing raw material mixes; Get sodium carboxymethyl cellulose again, adding distil water leaves standstill 24h, makes its abundant swelling; Medicine is slowly added in the gel-type vehicle in stirring down, and adding distil water promptly gets gel to capacity.
Embodiment 3: 6 parts of Bay leaves oils, 7 parts of cinnamic aldehyde, 3 parts of citronella oil, 28 parts of Oleum Terebinthinaes, 75 parts of wintergreen oils
Take by weighing said medicine and be dissolved in 600～700ml, 75% ethanol, after the dissolving fully, add 75% ethanol and add to 1000ml, shake up, filter and promptly get tincture.
Embodiment 4: 5 parts of Bay leaves oils, 6 parts of cinnamic aldehyde, 2.5 parts of citronella oil, 27 parts of Oleum Terebinthinaes, 72 parts of wintergreen oils
Take by weighing said medicine and add dissolve with ethanol, be sub-packed in the spray device of regulation, promptly get spray.
Embodiment 5: 2.5 parts of Bay leaves oils, 3.2 parts of cinnamic aldehyde, 1.5 parts of citronella oil, 19 parts of Oleum Terebinthinaes, 63 parts of wintergreen oils
Take by weighing said medicine and add dissolve with ethanol, be sub-packed in the container of regulation, promptly get varnish, gauze or Cotton Gossypii are dipped in and get and be applied to the affected part before using.
Embodiment 6 discrimination methods:
(1) it is an amount of to get this product, adds anhydrous alcohol solution, makes the solution that every ml contains 10mg, as need testing solution; Get australene, the methyl salicylate reference substance is an amount of, add dehydrated alcohol and make the solution that every ml contains 5mg, in contrast product solution; Gas chromatography determination, test sample chromatograph should present the chromatographic peak identical with the reference substance chromatographic retention;
(2) it is an amount of to get this product, adds anhydrous alcohol solution, makes the solution that every ml contains 10mg, as need testing solution; It is an amount of that other gets the cinnamic aldehyde reference substance, adds anhydrous alcohol solution, makes the reference substance solution that every ml contains 1mg; According to the thin layer chromatography test, draw above-mentioned two kinds of solution each 5 μ l, 4 μ l, put respectively on same silica gel g thin-layer plate, with petroleum ether: ethyl ester acetic acid=17: 3 is developing solvent, launch, take out, dry, spray is with the dinitrophenylhydrazine alcoholic solution, 105 ℃ were dried by the fire 5 minutes, and inspected under the daylight, in the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.
Embodiment 7 content assaying methods:
SE-54 is an immobile phase with phenyl ethylene methyl silicone rubber; Coating concentration is 10%, is carrier with Chromosorb WAW-DMCS 60-80 order; Temperature programming, 80 ℃ of initial temperatures, per minute heats up 15 ℃, 210 ℃ of final temperatures; Take by weighing respectively that australene is rare, the methyl salicylate reference substance is an amount of, add dehydrated alcohol and make the solution that every 1ml contains 5mg, in contrast product solution; Get this product 50mg, the accurate title, decide, and puts in the 5ml measuring bottle, adds dehydrated alcohol and be diluted to scale, as need testing solution; Precision is measured reference substance solution and each 1 μ l of need testing solution respectively, and inject gas chromatograph is measured, calculated weight percentage ratio, and this product contains australene should be lower than 16.5%, contains methyl salicylate and should be 60.3-73.7%.
Claims (8)
1. the external preparation of a removing obstruction in the collateral to relieve pain, it is characterized in that: it is made up of following raw materials according: Bay leaves oil 2-6 part, cinnamic aldehyde 3-7 part, citronella oil 1-3 part, Oleum Terebinthinae 18-28 part, wintergreen oil 60-75 part.
2. according to the described external preparation of claim 1, it is characterized in that: it is made up of following raw materials according: 4 parts of Bay leaves oils, 5 parts of cinnamic aldehyde, 2 parts of citronella oil, 22 parts of Oleum Terebinthinaes, 67 parts of wintergreen oils.
3. according to the preparation method of claim 1 or 2 described external preparation, it is characterized in that: the raw material of getting it filled, make multiple exterior-applied formulation respectively, comprising: medicine oil, unguentum, gel, lotion, spray, patch, tincture.
4. according to the preparation method of the described external preparation of claim 3, it is characterized in that: take by weighing Bay leaves oil, cinnamic aldehyde, citronella oil, Oleum Terebinthinae and stir, add the wintergreen oil dilution, promptly get medicine oil.
5. according to the detection method of claim 1 or 2 described external preparation, it is characterized in that: discrimination method comprises following all or part of content:
(1) adopt gas chromatography, contrast with australene, methyl salicylate reference substance, this product shows the chromatographic peak identical with the reference substance retention time;
(2) adopt thin layer chromatography, contrast, on this product and the corresponding position of reference substance chromatograph, show the speckle of same color with the cinnamic aldehyde reference substance.
6. according to the detection method of the described external preparation of claim 5, it is characterized in that: discrimination method comprises following all or part of content:
(1) it is an amount of to get this product, adds anhydrous alcohol solution, makes need testing solution; Get australene, the methyl salicylate reference substance is an amount of, add dehydrated alcohol and make reference substance solution; Gas chromatography determination, test sample chromatograph should present the chromatographic peak identical with the reference substance chromatographic retention;
(2) it is an amount of to get this product, adds anhydrous alcohol solution, makes need testing solution; It is an amount of that other gets the cinnamic aldehyde reference substance, adds anhydrous alcohol solution, makes reference substance solution; According to the thin layer chromatography test, draw above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, with petroleum ether: ethyl ester acetic acid=17: 3 is developing solvent, launch, take out, dry, spray is with the dinitrophenylhydrazine alcoholic solution, 105 ℃ were dried by the fire 5 minutes, and inspected under the daylight, in the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.
7. according to the detection method of claim 1 or 2 described external preparation, it is characterized in that: assay employing gas chromatography determination australene is rare, the content of methyl salicylate.
8. according to the detection method of the described external preparation of claim 7, it is characterized in that: assay adopts gas chromatography:
Take by weighing respectively that australene is rare, the methyl salicylate reference substance is an amount of, add dehydrated alcohol and make reference substance solution; Get this product, the accurate title, decide, and puts in the measuring bottle, adds the dehydrated alcohol dilution, as need testing solution; Precision is measured reference substance solution and each 1 μ l of need testing solution respectively, and inject gas chromatograph is measured, calculated weight percentage ratio, and this product contains australene should be lower than 16.5%, contains methyl salicylate and should be 60.3-73.7%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101118290A CN102151311A (en) | 2010-02-11 | 2010-02-11 | External preparation with functions of removing obstruction in channels and relieving pain, preparation method and detection method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101118290A CN102151311A (en) | 2010-02-11 | 2010-02-11 | External preparation with functions of removing obstruction in channels and relieving pain, preparation method and detection method thereof |
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| CN102151311A true CN102151311A (en) | 2011-08-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2010101118290A Pending CN102151311A (en) | 2010-02-11 | 2010-02-11 | External preparation with functions of removing obstruction in channels and relieving pain, preparation method and detection method thereof |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1443563A (en) * | 2003-03-18 | 2003-09-24 | 彭少明 | Huoluo oil |
| CN1454659A (en) * | 2003-03-18 | 2003-11-12 | 彭少明 | Safflower oil |
| CN1951483A (en) * | 2006-11-21 | 2007-04-25 | 陈国基 | Hybrid safflower oil and preparation method thereof |
-
2010
- 2010-02-11 CN CN2010101118290A patent/CN102151311A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1443563A (en) * | 2003-03-18 | 2003-09-24 | 彭少明 | Huoluo oil |
| CN1454659A (en) * | 2003-03-18 | 2003-11-12 | 彭少明 | Safflower oil |
| CN1951483A (en) * | 2006-11-21 | 2007-04-25 | 陈国基 | Hybrid safflower oil and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 梁延寿 等: "《现代中药制剂检验技术》", 30 September 2004, article ""中药制剂检测特点"，"气相色谱鉴别法"，"薄层色谱鉴别法"" * |
| 武彦文 等: "《当代中国近红外光谱技术——全国近红外外光谱学术会议论文集》", 31 December 2006, article "近红外光谱法解析正红花油的组成" * |
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Application publication date: 20110817 |