CN1951221A - Method for preparing selenium enriched oyster mushroom powder - Google Patents
Method for preparing selenium enriched oyster mushroom powder Download PDFInfo
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- CN1951221A CN1951221A CNA2006101352272A CN200610135227A CN1951221A CN 1951221 A CN1951221 A CN 1951221A CN A2006101352272 A CNA2006101352272 A CN A2006101352272A CN 200610135227 A CN200610135227 A CN 200610135227A CN 1951221 A CN1951221 A CN 1951221A
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- oyster mushroom
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Abstract
The invention relates to a method for preparing selenium-abundant mushroom powder. Wherein, it plants the mushroom seed into PDA culture medium to cultivate seed in tube; plants tube seed into PDA selenium-abundant culture medium to process solid product seed cultivation; cultivating the solid product seed in PDB selenium-abundant container to process solid mother cultivation; plants liquid mother into PDB selenium-abundant culture medium to process selenium-abundant little mushroom cultivation; separates the selenium-abundant little mushroom from culture medium; drying and breaking into powder. The invention has high culture speed and low material consumption. And the product has high organic selenium convert ability, and high selenium content.
Description
Technical field
The present invention relates to edible fungus liquid and cultivate and culture medium prescription, more specifically relate to a kind of preparation method of selenium enriched oyster mushroom powder.
Background technology
In recent years, China's edible mushroom technical development was very fast, and particularly the selenium-enriched edible mushroom culture technique is cultivated (Ye Huafu, publication No. CN1213488A, " Se-rich xianggu additive ") as Se-rich xianggu.Selenium is the necessary nutritional trace element of human body, and some the important metabolic process that takes place in its participant soma has propagation, the differentiation of regulating cancer cell, the effect that the malignant phenotype is reversed.The edible mushroom technique of enriched selenium is that the intermediate product with the edible mushroom metabolic conversion is a carrier, adds selenium and carries out biology and strengthen, and the edible mushroom of developing rich selenium can be used as organic selemium nutrition tonic that people's physical efficiency absorbs.Elegant precious mushroom is seen everywhere on market as edible mushroom, and this kind edible mushroom shape is small and exquisite, and is delicious in taste, very popular.The selenium enriched oyster mushroom that the precious mushroom of show carries out after rich selenium is cultivated is not only delicious, and have the body-building health function of additional human body selenium element, but problem such as present rich selenium mushroom culture technique also exists edible fungi growth speed slow, and few, the anti-selenium ability of rich selenium amount is weak.
Summary of the invention
The present invention seeks to provides a kind of preparation method of selenium enriched oyster mushroom powder at existing problem in the existing rich selenium mushroom culture technique.
The preparation method of selenium enriched oyster mushroom powder of the present invention comprises:
(1): adopt in the PDA culture medium of oyster mushroom kind access after sterilization treatment and carry out the test tube strains cultivation;
(2): the test tube strains that step 1 is cultivated inserts carries out the solids manufacture kind and cultivates in the container that the PDA enriched medium after sterilization treatment is housed;
(3): the solids manufacture kind that step 2 is cultivated inserts carries out female kind of liquid and cultivates in the container that the PDB enriched medium after sterilization treatment is housed; The condition of culture of described cultivation is cultivated for the vibration deep layer;
(4): the liquid that step 3 is cultivated is female plants to insert and carries out the selenium enriched oyster mushroom silk in the container that the PDB Jia Fufu seleno culture medium after sterilization treatment is housed and cultivate; Described PDB Jia Fufu seleno culture medium is made by the PDB enriched medium that is added with sodium selenite, and the final concentration of sodium selenite is 1.5～20.0 μ g/ml;
(5): the selenium enriched oyster mushroom silk that step 4 is cultivated is separated from culture medium, made selenium enriched oyster mushroom powder at last through super-dry, pulverizing.
Advantage of the present invention is: the selenium enriched oyster mushroom silk of producing with this preparation method need not experience very long mycelia and walk bag, fruiting stage; The Liquid Culture mycelial growth rate is fast, and the cultivation resource consumption is few, can realize the suitability for industrialized production requirement; The organic selenium conversion capability of the selenium enriched oyster mushroom that is produced is strong, rich selenium degree height, and anti-selenium ability is strong, problem such as it is slow to have solved in the existing rich selenium mushroom culture technique edible fungi growth speed, and few, the anti-selenium ability of rich selenium amount is weak.
The specific embodiment
Adopt in the PDA culture medium of oyster mushroom kind access after sterilization treatment and carry out the test tube strains cultivation; Test tube strains inserted carry out the solid kind in the PDA enriched medium be equipped with after sterilization treatment and cultivate; The solids manufacture kind inserted carry out female kind of liquid in the container that the PDB enriched medium after sterilization treatment is housed and cultivate, its condition of culture is cultivated for the vibration deep layer, and vibration deep layer condition of culture is a temperature 25 ℃ ± 0.5, rotating speed 150 ± 2r/min; With liquid female plant to insert carry out the selenium enriched oyster mushroom silk in the container that the PDB Jia Fufu seleno culture medium after sterilization treatment is housed and cultivate, PDB Jia Fufu seleno culture medium is made by the PDB enriched medium that is added with sodium selenite, and the final concentration of sodium selenite is 1.5～20.0 μ g/ml; The selenium enriched oyster mushroom silk is separated from culture medium, made selenium enriched oyster mushroom powder at last through super-dry, pulverizing.
In the preparation process, after the selenium enriched oyster mushroom silk must be washed, separate through distilled water, low-temperature reduced-pressure drying, low-temperature grinding were made selenium enriched oyster mushroom powder again, through distilled water washing, the number of times that separates greater than 3 times.
The bacterial classification in step 1, step 2 and the step 3 and the volume ratio of culture medium are 5～15%.
Used PDA enriched medium adds wheat bran by the PDA culture medium and dusty yeast constitutes in the preparation, in every 100ml constant volume culture medium, raw materials usedly is: potato 20g, glucose 2g, wheat bran 1g, KH
2PO
40.3g, MgSO
40.15g, V
B10.01g, dusty yeast 0.15g, agar 2g; The compound method of PDA enriched medium is: potato that should add and wheat bran amount and a certain proportion of distilled water mixing earlier, keep filtering behind the boiling 30min, add other raw material and fully mix after be settled to volume required.
PDB enriched medium prescription used in the preparation is: in every 100ml constant volume culture medium, raw materials usedly be: potato 20g, glucose 2g, wheat bran 1g, KH
2PO
40.3g, MgSO
40.15g, V
B10.01g, dusty yeast 0.15g; The compound method of PDB enriched medium is: potato that should add and wheat bran amount and a certain proportion of distilled water mixing earlier, filter after keeping hubble-bubble to rise 30min, add other raw material and fully mix after be settled to volume required.
Embodiment
The cultivation of test tube strains: the oyster mushroom strain is inserted PDA medium preparation test tube strains;
The plate kind is cultivated: get fritter test tube kind and insert in the plate that sterilization PDA enriched medium is housed, be statically placed in 25 ℃ of constant incubators and cultivate, cover with the plate media surface to mycelia.
The female kind of liquid cultivated: the plate kind of getting 5 0.8cm diameters inserts in the triangle blake bottle that sterilization PDB enriched medium is housed; The deep layer of wherein vibrating condition of culture is: 25 ℃ ± 0.5, and rotating speed 150 ± 2r/min.
Rich selenium cultural hypha: meet mother by 10% liquid strain amount and plant in the triangle blake bottle that sterilization PDB Jia Fufu seleno culture medium is housed, the deep layer of wherein vibrating condition of culture is: 25 ℃ ± 0.5, and rotating speed 150 ± 2r/min.
Selenium enriched oyster mushroom powder preparation: the selenium enriched oyster mushroom silk is separated from culture medium,, made selenium enriched oyster mushroom powder at last through low-temperature reduced-pressure drying, low-temperature grinding again through 3 washings, separation.
The PDA enriched medium adds wheat bran by the PDA culture medium and dusty yeast constitutes, the PDA enriched medium compound method of 100ml is: with the distilled water mixing of potato 20g and 1g wheat bran and certain proportion (being less than 100ml), keep filtering behind the boiling 30min, add glucose 2g, KH
2PO
40.3g, MgSO
40.15g, V
B10.01g, dusty yeast 0.15g, agar 2g is settled to 100ml after fully mixing.
The preparation method of the PDB enriched medium of 100ml is: with the distilled water mixing of potato 20g and 1g wheat bran and certain proportion (being less than 100ml), keep filtering behind the boiling 30min, add glucose 2g, KH
2PO
40.3g, MgSO
40.15g, V
B10.01g dusty yeast 0.15g is settled to 100ml after fully mixing.
PDB Jia Fufu seleno culture medium is made by the PDB enriched medium that is added with sodium selenite, and the final concentration of sodium selenite is 10.0 μ g/ml.
Claims (8)
1. the preparation method of a selenium enriched oyster mushroom powder, its principal character is: preparation according to the following steps:
(1): adopt in the PDA culture medium of oyster mushroom kind access after sterilization treatment and carry out the test tube strains cultivation;
(2): the test tube strains that step 1 is cultivated inserts carries out the solids manufacture kind and cultivates in the container that the PDA enriched medium after sterilization treatment is housed;
(3): the solids manufacture kind that step 2 is cultivated inserts carries out female kind of liquid and cultivates in the container that the PDB enriched medium after sterilization treatment is housed; The condition of culture of described cultivation is cultivated for the vibration deep layer;
(4): the liquid that step 3 is cultivated is female plants to insert and carries out the selenium enriched oyster mushroom silk in the container that the PDB Jia Fufu seleno culture medium after sterilization treatment is housed and cultivate; Described PDB Jia Fufu seleno culture medium is made by the PDB enriched medium that is added with sodium selenite, and the final concentration of sodium selenite is 1.5～20.0 μ g/ml;
(5): the selenium enriched oyster mushroom silk that step 4 is cultivated is separated from culture medium, made selenium enriched oyster mushroom powder at last through super-dry, pulverizing.
2. according to the preparation method of the described selenium enriched oyster mushroom powder of claim 1, it is characterized in that: after described selenium enriched oyster mushroom silk must be washed, separate through distilled water, low-temperature reduced-pressure drying, low-temperature grinding were made selenium enriched oyster mushroom powder again.
3. according to the preparation method of the described selenium enriched oyster mushroom powder of claim 2, it is characterized in that: described selenium enriched oyster mushroom silk through distilled water washing, the number of times that separates greater than 3 times.
4. according to the preparation method of the described selenium enriched oyster mushroom powder of claim 1, it is characterized in that: the bacterial classification in described step 1, step 2 and the step 3 and the volume ratio of culture medium are 5～15%.
5. according to the preparation method of the described selenium enriched oyster mushroom powder of claim 1, it is characterized in that: described preparation method's vibration deep layer condition of culture is a temperature 25 ℃ ± 0.5, rotating speed 150 ± 2r/min.
6. according to the preparation method of the described selenium enriched oyster mushroom powder of claim 1, it is characterized in that: described PDA enriched medium adds wheat bran by the PDA culture medium and dusty yeast constitutes.
7. according to the preparation method of the described selenium enriched oyster mushroom powder of claim 6, it is characterized in that: described PDA enriched medium prescription is: in every 100ml constant volume culture medium, raw materials usedly be: potato 20g, glucose 2g, wheat bran 1g, KH
2PO
40.3g, MgSO
40.15g, V
B10.01g, dusty yeast 0.15g, agar 2g; The compound method of described PDA enriched medium is: potato that should add and wheat bran amount and a certain proportion of distilled water mixing earlier, keep filtering behind the boiling 30min, add other raw material and fully mix after be settled to volume required.
8. according to the preparation method of the described selenium enriched oyster mushroom powder of claim 1, it is characterized in that: described PDB enriched medium prescription is: in every 100ml constant volume culture medium, raw materials usedly be: potato 20g, glucose 2g, wheat bran 1g, KH
2PO
40.3g, MgSO
40.15g, V
B10.01g, dusty yeast 0.15g; The compound method of described PDB enriched medium is: potato that should add and wheat bran amount and a certain proportion of distilled water mixing earlier, filter after keeping hubble-bubble to rise 30min, add other raw material and fully mix after be settled to volume required.
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| CN100493379C CN100493379C (en) | 2009-06-03 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150877A (en) * | 2011-03-21 | 2011-08-17 | 福建省农业科学院农业工程技术研究所 | Method for preparing selenium-rich dried pork slices by utilizing selenium-rich edible fungus powder |
| CN103005437A (en) * | 2012-12-17 | 2013-04-03 | 福建省农业科学院农业工程技术研究所 | Method for preparing Se-enriched bran edible mushroom nutrition powder by solid cultivation |
| CN103798412A (en) * | 2014-03-12 | 2014-05-21 | 兰爱珍 | Instant selenium-enriched soya-bean milk skin and preparation method thereof |
| CN104382054A (en) * | 2014-10-30 | 2015-03-04 | 福建省农业科学院农业工程技术研究所 | Preparation method of high-fiber and selenium-enriched multi-element nutritional mushroom decoction pieces |
| CN104397720A (en) * | 2014-10-29 | 2015-03-11 | 贵州安森生物科技有限公司 | Technological process for efficiently rapidly cultivating organic biological selenium |
| CN104473141A (en) * | 2014-11-04 | 2015-04-01 | 苏州硒泰克生物科技有限公司 | Method for producing organic selenium-rich additive by using high selenium edible fungi |
| CN107810779A (en) * | 2017-09-30 | 2018-03-20 | 重庆市宽哥农业发展有限公司 | The breeding method of the elegant precious mushroom of refuse reclamation |
| CN109355243A (en) * | 2018-11-15 | 2019-02-19 | 海门颐达医药科技有限公司 | The preparation method and applications of the selenium-rich schizophyllum commune of high yield glucan |
| CN114402908A (en) * | 2022-01-20 | 2022-04-29 | 福建农林大学 | Efficient pleurotus geesteranus fruiting cultivation method by hanging bag marking |
Family Cites Families (2)
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|---|---|---|---|---|
| CN1097025A (en) * | 1993-06-30 | 1995-01-04 | 云南省农业科学院粮食作物研究所 | Prescription of culture liquid for germanium-rich glossy ganoderma |
| CN1093736C (en) * | 1998-04-28 | 2002-11-06 | 山东省滨州卫生学校 | Method for cultivating selenium-rich glossy ganoderma |
-
2006
- 2006-11-14 CN CNB2006101352272A patent/CN100493379C/en not_active Expired - Fee Related
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150877A (en) * | 2011-03-21 | 2011-08-17 | 福建省农业科学院农业工程技术研究所 | Method for preparing selenium-rich dried pork slices by utilizing selenium-rich edible fungus powder |
| CN102150877B (en) * | 2011-03-21 | 2013-01-02 | 福建省农业科学院农业工程技术研究所 | Method for preparing selenium-rich dried pork slices by utilizing selenium-rich edible fungus powder |
| CN103005437A (en) * | 2012-12-17 | 2013-04-03 | 福建省农业科学院农业工程技术研究所 | Method for preparing Se-enriched bran edible mushroom nutrition powder by solid cultivation |
| CN103005437B (en) * | 2012-12-17 | 2014-03-12 | 福建省农业科学院农业工程技术研究所 | Method for preparing Se-enriched bran edible mushroom nutrition powder by solid cultivation |
| CN103798412A (en) * | 2014-03-12 | 2014-05-21 | 兰爱珍 | Instant selenium-enriched soya-bean milk skin and preparation method thereof |
| CN103798412B (en) * | 2014-03-12 | 2015-06-10 | 兰爱珍 | Instant selenium-enriched soya-bean milk skin and preparation method thereof |
| CN104397720A (en) * | 2014-10-29 | 2015-03-11 | 贵州安森生物科技有限公司 | Technological process for efficiently rapidly cultivating organic biological selenium |
| CN104397720B (en) * | 2014-10-29 | 2016-08-24 | 贵州安森生物科技有限公司 | The efficiently technological process of quickly breeding organic-biological selenium |
| CN104382054A (en) * | 2014-10-30 | 2015-03-04 | 福建省农业科学院农业工程技术研究所 | Preparation method of high-fiber and selenium-enriched multi-element nutritional mushroom decoction pieces |
| CN104382054B (en) * | 2014-10-30 | 2016-07-06 | 福建省农业科学院农业工程技术研究所 | A kind of preparation method of high fine selenium-rich multielement nutrition mushroom decoction pieces |
| CN104473141A (en) * | 2014-11-04 | 2015-04-01 | 苏州硒泰克生物科技有限公司 | Method for producing organic selenium-rich additive by using high selenium edible fungi |
| CN107810779A (en) * | 2017-09-30 | 2018-03-20 | 重庆市宽哥农业发展有限公司 | The breeding method of the elegant precious mushroom of refuse reclamation |
| CN109355243A (en) * | 2018-11-15 | 2019-02-19 | 海门颐达医药科技有限公司 | The preparation method and applications of the selenium-rich schizophyllum commune of high yield glucan |
| CN109355243B (en) * | 2018-11-15 | 2022-01-25 | 海门颐达医药科技有限公司 | Preparation method and application of selenium-rich schizophyllum commune for high yield of glucan |
| CN114402908A (en) * | 2022-01-20 | 2022-04-29 | 福建农林大学 | Efficient pleurotus geesteranus fruiting cultivation method by hanging bag marking |
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| CN100493379C (en) | 2009-06-03 |
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