CN1928099A

CN1928099A - Method of transforming abandoned biomass to acetic acid by two-phase coupling process of hydrogen-producing acid-producing and hydrogen-consuming hydrogen-producing

Info

Publication number: CN1928099A
Application number: CN 200610041457
Authority: CN
Inventors: 陈坚; 刘和; 聂艳秋; 堵国成
Original assignee: Jiangnan University
Current assignee: Jiangnan University
Priority date: 2006-09-05
Filing date: 2006-09-05
Publication date: 2007-03-14
Anticipated expiration: 2026-09-05
Also published as: CN100595279C

Abstract

The two phase coupling process combining hydrogen-producing acid-producing phase and hydrogen-consuming acid-producing phase to convert waste biomass into acetic acid belongs to the field of waste biomass resource utilizing technology. The process includes the anaerobic fermentation of waste biomass in hydrogen-producing acid-producing phase with active sludge as seed sludge and glucose as template substrate to produce acetic acid in liquid, hydrogen and CO2; and the homotypic acidification in hydrogen-consuming acid-producing phase with the gases produced in the hydrogen-producing acid-producing phase as substrate to produce acetic acid. The process has acetic acid yield of 0.39 g each gram of glucose, 40-100 % higher than that with only hydrogen-consuming acid-producing phase.

Description

It is a kind of that hydrogen produces acid and consumption hydrogen produces the method that sour two-phase coupling process is converted into abandoned biomass acetate with producing

Technical field

A kind ofly produce acid and consumption hydrogen and produce sour two-phase coupling process abandoned biomass is converted into the method for acetate, belong to abandoned biomass resource utilization field, relate to a kind of method that abandoned biomass is converted into acetate with producing hydrogen.

Background technology

The anaerobic digestion process of abandoned biomass (as: organic composition in municipal sludge, high concentrated organic wastewater, the municipal garbage etc.) generally can be divided into product acid and produce two stages of methane, research to the abandoned biomass anaerobic digestion, biased toward mostly in the past how to make abandoned biomass to greatest extent stabilization and the more methane of output as fuel, but because methane purifies and the storage investment is big, fail to obtain fine utilization, cause energy dissipation and greenhouse gases to increase.Also there is at present pair abandoned biomass to carry out the research report that anaerobe produces hydrogen, yet the intermediate product of anaerobic digestion process---organic acid is to have more high value-added product, and anaerobic digestion process is controlled at the product acid phase and then obtains organic acid will be more valuable.

Summary of the invention

The purpose of this invention is to provide a kind of with producing the acid of hydrogen product and consuming hydrogen and produce the method that sour two-phase coupling process is converted into abandoned biomass acetate, present method is a kind of technology that abandoned biomass is converted into acetate of strengthening, produce acid and consume the coupling that hydrogen produces sour two-phase by producing hydrogen, promote the degraded of abandoned biomass, improve the productive rate of acetate, the road of a more valuable resource utilization is provided for abandoned biomass.

Technical scheme: use the anaerobic activated sludge of crossing through heat treated to make kind of a mud, in producing the acid mutually of hydrogen product, carry out anaerobically fermenting in glucose operation mode substrate, make the organic acid that mainly comprises acetate obtain accumulation with abandoned biomass; In consuming the acid mutually of hydrogen product, also use the anaerobic digestion active sludge of crossing through heat treated to make kind of a mud, simultaneously, in the acid mutually of consumption hydrogen product, carry out the homotype acetification as substrate, generation acetate with the biogas (hydrogen and carbonic acid gas) that the acid of product hydrogen product produces mutually.

The thermal treatment of anaerobic digestion active sludge and activation: it is air-dry to get the anaerobic digestion active sludge, grinds, and sieves, and at 105 ℃ of heating 2h, kills non-sporeformer methanogen.The anaerobic digestion active sludge that the heat treated of learning from else's experience is crossed adds work kind of a mud in the 3g/L glucose nutrient solution, and planting mud concentration is 10-20g VS/L, transfers pH to 6.5, and 35 ℃ activate 24h down.

The condition of acetate is produced in coupling:

Produce hydrogen and produce sour phase: abandoned biomass is 25-35g/L in the glucose meter, plants mud concentration 2-8g VS/L, initial pH7.8-8.2.

Consumption hydrogen produces sour phase: plant mud concentration 2-8g VS/L, initial pH6.2-6.6.

Fermentation condition: inflated with nitrogen 1-5min, seal 30-40 ℃ of bottom fermentation 10-15d.Adopt shaker fermentation, rotating speed is 120r/min.

Beneficial effect of the present invention: utilize abandoned biomass to make raw material, hydrogen produces acid and consumption hydrogen produces sour two-phase coupling by producing, and obtains high value added product acetate.First advantage is the problem that can solve easy degradation product environmental pollution in the abandoned biomass, simultaneously recyclable high value added product acetate; Second largest advantage is to make product hydrogen produce the sour biogas that produces mutually to be consumed hydrogen product acid absorption rapidly mutually, increased the productive rate of acetate greatly, the acetate productive rate reaches as high as 0.39g acetate/g glucose, produces the relative substrate (with glucose meter) of hydrogen product acid than independent utilization and carries out acidifying acetate productive rate raising 40-100%; Characteristics such as the third-largest advantage is only need add a dividing plate in general anaerobic digestion reaction vessel, can form the biphasic reaction device, and it is simple, easy to operate to have equipment, and cost is low.

Description of drawings

Fig. 1 experimental installation of the present invention.

1, thief hole; 2, injection port; 3, pipe connecting; A, product hydrogen produce sour phase; H, consumption hydrogen produce sour phase.

Embodiment

Further describe the present invention below in conjunction with drawings and Examples, but be not subjected to the restriction of drawings and Examples.

Embodiment 1:

The thermal treatment of anaerobic digestion active sludge and activation: it is air-dry to get the anaerobic digestion active sludge, grind, sieve, heat 2h down at 105 ℃, kill non-sporeformer methanogen, the anaerobic digestion active sludge that the heat treated of learning from else's experience is crossed adds in the 3g/L glucose nutrient solution, and planting mud concentration is 10-20g VS/L, transfer pH to 6.5,35 ℃ activate 24h down.

Embodiment 2:

The substrate abandoned biomass is 30g/L with glucose meter concentration in the A bottle, planting mud concentration is 8g VS/L, H bottle kind mud concentration is 2g VS/L, at 35 ℃ of bottom fermentation 10d, A bottle acetic acid concentration 8.47 ± 0.17g/L, H bottle acetic acid concentration 0.44 ± 0.01g/L, acetate proportion in the fermentation liquid-phase product is 56.3 ± 0.9%, the acetate productive rate is 0.30 ± 0.01g acetate/g glucose.

Embodiment 3:

The substrate abandoned biomass is 25g/L with glucose meter concentration in the A bottle, planting mud concentration is 2g VS/L, H bottle kind mud concentration is 4g VS/L, at 40 ℃ of bottom fermentation 13d, A bottle acetic acid concentration 9.57 ± 0.30g/L, H bottle acetic acid concentration 0.61 ± 0.01g/L, acetate proportion in the fermentation liquid-phase product is 55.9 ± 0.8%, the acetate productive rate is 0.34 ± 0.01g acetate/g glucose.

Embodiment 4:

The substrate abandoned biomass is 35g/L with glucose meter concentration in the A bottle, planting mud concentration is 4g VS/L, H bottle kind mud concentration is 8g VS/L, at 30 ℃ of bottom fermentation 15d, A bottle acetic acid concentration 11.20 ± 0.52g/L, H bottle acetic acid concentration 0.62 ± 0.06g/L, acetate proportion in the fermentation liquid-phase product is 62.2 ± 4.8%, the acetate productive rate is 0.39 ± 0.02g acetate/g glucose.

Claims

It is 1, a kind of that hydrogen produces acid and consumption hydrogen produces the method that sour two-phase coupling process is converted into abandoned biomass acetate with producing, it is characterized in that in producing the acid mutually of hydrogen product, using the anaerobic digestion active sludge of crossing through heat treated to make kind of a mud, carry out anaerobically fermenting with abandoned biomass in glucose operation mode substrate, produce the liquid product and biogas hydrogen and the carbonic acid gas that mainly comprise acetate; In consuming the acid mutually of hydrogen product, also use the anaerobic digestion active sludge of crossing through heat treated to make kind of a mud, carry out the homotype acetification as substrate, be converted into acetate with the biogas that the acid of product hydrogen product produces mutually;

(1) thermal treatment of anaerobic digestion active sludge and activation: it is air-dry to get the anaerobic digestion active sludge, grind, sieve, at 105 ℃ of heating 2h, kill non-sporeformer methanogen, the anaerobic digestion active sludge that the heat treated of learning from else's experience is crossed adds in the 3g/L glucose nutrient solution makes kind of a mud, and planting mud concentration is 10-20g VS/L, transfer pH to 6.5,35 ℃ activate 24h down;

(2) condition of acetate is produced in coupling:

Produce hydrogen and produce sour phase: abandoned biomass is 25-35g/L with the glucose meter, plants mud concentration 2-8g VS/L, initial pH7.8-8.2;

Consumption hydrogen produces sour phase: plant mud concentration 2-8g VS/L, initial pH6.2-6.6;

Fermentation condition: inflated with nitrogen 1-5min, seal, 30-40 ℃ of bottom fermentation 10-15d adopts shaker fermentation, and rotating speed is 120r/min.