CN1924574A - Spot sample device - Google Patents
Spot sample device Download PDFInfo
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- CN1924574A CN1924574A CN 200610116183 CN200610116183A CN1924574A CN 1924574 A CN1924574 A CN 1924574A CN 200610116183 CN200610116183 CN 200610116183 CN 200610116183 A CN200610116183 A CN 200610116183A CN 1924574 A CN1924574 A CN 1924574A
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- spotting
- needle
- sample device
- spot sample
- arm
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Abstract
This invention provides one point sampling device, which comprises the following parts: load bench to support samples; sample arm fixed on the sample needle fixed on the said sample arm; motion structure connected to the sample arm to control the point arm or sample needle parallel and vertical to the load bench plane; high voltage power module coupled with point needle to form high pressure field between the point sample needle and sample surface to eject the point sample solution to the point sample surface.
Description
Technical field
The present invention relates to chemical analysis technology, particularly a kind of spot sample device based on the electron spray mode.
Background technology
Traditional point sample method, the point sample of thin layer plate for example, generally adopt three kinds of modes:
1, quantitative kapillary point sample: this point sample method point sample amount depends on quantitative solution capillaceous, and point sample, and each position arbitrarily can not be in full accord, has the point sample error, and can only put round dot;
2, mechanical spotting needle point sample: general spotting needle is that metal is made, and the surface is the solution of absorption point sample easily, and need be very approaching with the thin layer plate distance during point sample, is easy to poke thin layer plate, causes invalid point sample, and can only put round dot;
3, gas blowout cloud point sample: can put round dot and band, contactless, as shown in Figure 1.Generally all adopt this point sample mode abroad, point sample is more accurate.But the silica gel plate for no binder blows away silica white easily, causes the point sample failure; Owing to be gas atomization, solution diffusion is more serious in addition, causes the band can not be very thin.
Summary of the invention
The purpose of this invention is to provide a kind of spot sample device, it both can put round dot, can also put band, the noncontact point sample, and to the point sample no any damage in surface, and strip width can be accomplished very narrow.
Above-mentioned purpose of the present invention realizes by following technical proposal:
A kind of spot sample device comprises:
The objective table of all product of bearing point;
Spotting arm;
Be installed in the spotting needle on the described spotting arm;
With the motion that described spotting arm links to each other, be used to control described spotting arm or the motion of spotting needle in being parallel and perpendicular to the plane of described stage surface; And
With the high-voltage power module that described spotting needle lotus root is closed, be used between described spotting needle and described point sample sample surfaces, forming high-voltage electric field the spotting solution in the described spotting needle is injected into described point sample sample surfaces.
Preferably, in above-mentioned spot sample device, described spotting needle comprises:
The spotting needle glass tube is used to hold described spotting solution;
Can be along the reciprocating push rod of described spotting needle glass tube inwall;
Insulation nozzle; And
The metal needle tubing that two ends are connected with described spotting needle glass tube and described insulation nozzle respectively, its sidewall closes through lead and described high-voltage power module lotus root.
Preferably, in above-mentioned spot sample device, the endpiece of described insulation nozzle is cone shape.
Preferably, in above-mentioned spot sample device, the internal diameter of described insulation nozzle is less than 0.1 millimeter.
Preferably, in above-mentioned spot sample device, described insulation nozzle adopting quartz glass is made.
Preferably, in above-mentioned spot sample device, described motion comprises transverse movement mechanism and lengthwise movement mechanism, controls the motion in being parallel and perpendicular to the plane of described stage surface of described spotting arm or spotting needle respectively.
Preferably, in above-mentioned spot sample device, described transverse movement mechanism and lengthwise movement mechanism are screw body.
Preferably, in above-mentioned spot sample device, form described high-voltage electric field by on described spotting needle, applying the controlled DC voltage of voltage waveform.
Preferably, in above-mentioned spot sample device, the repetition frequency of described voltage waveform is 500Hz.
Compare with traditional point sample technology, adopt the spot sample device of said structure not have any infringement for the layer of silica gel on thin layer plate surface, can put round dot and band, point sample speed and band length all can freely be set, and the minimum widith of band can be controlled in 0.2mm, so narrow strip width is fairly obvious for the raising effect of test sample degree of separation, and is very helpful for the research unit that pharmaceutical industry is relevant with each.
Description of drawings
By below in conjunction with the description of accompanying drawing to preferred embodiment of the present invention, can further understand purpose of the present invention, feature and advantage, wherein:
Fig. 1 is the structural representation according to the spot sample device of one embodiment of the invention.
Fig. 2 shows the electron spray principle.
Fig. 3 is the DC voltage change curve that spot sample device adopted according to one embodiment of the invention.
Embodiment
Spot sample device of the present invention comprises spotting arm, objective table, transverse movement mechanism, lengthwise movement mechanism, high-voltage power module, control system and spotting needle etc., below is further described for each component units.
Spotting arm is the critical piece of spot sample device, and it links to each other with lengthwise movement mechanism with transverse movement mechanism, therefore can move in the plane that is being parallel and perpendicular to objective table under the drive of these two mechanisms respectively.Particularly, the length of the band of point sample and position can be adjusted by the control spotting arm by transverse movement mechanism, and point sample speed and point sample amount can be regulated by control spotting arm or the lengthwise movement that is installed in the spotting needle on the spotting arm by lengthwise movement mechanism.Horizontal and vertical motion for example can adopt screw mechanism to realize, particularly for the point sample of band and round dot.
Spotting needle is installed on the spotting arm, and Fig. 1 shows the structure of spotting needle.As shown in Figure 1, spotting needle 1 comprises push rod 11, spotting needle glass tube 12, metal needle tubing 13 and insulation nozzle 14.Push rod 11 is positioned at a wherein end port of spotting needle glass tube 12, can be reciprocating along the inwall of spotting needle glass tube 12, thus the spotting solution 4 in the spotting needle glass tube 12 is pushed insulation nozzle 14.The two ends of metal needle tubing 13 are nested into spotting needle glass tube 12 other end ports and insulation nozzle 14 respectively, and sidewall closes through lead and high-voltage power module (not shown) lotus root.But insulation nozzle 14 for example adopting quartz glass is made.
As shown in Figure 1, carry point sample sample 3 on the objective table 2, for example the thin-layer silicon offset plate.When on metal needle tubing 13, applying a high direct voltage, to between metal needle tubing 13 and objective table 2, form high-voltage electric field, therefore push the solution generation ionization in the insulation nozzle 14, thereby the exit at nozzle forms spraying, along with the motion of spotting arm, this spraying arrives the point of sample that the point sample sample surfaces of placing on the objective table 2 promptly forms different shape.
Fig. 2 shows the principle of electron spray.As shown in Figure 2, the high-voltage electric field between metal needle tubing 13 and the objective table 2 makes solution generation ionization, and negative ion wherein attracted to the other end of metal needle tubing 13, then assembles a large amount of positive charge ions at the semi-moon shaped liquid surface of needle point.Repel mutually between positive charge ion, and expand away from the liquid surface of needle point, along with diminishing of droplet, electric field intensity strengthens gradually, and superfluous positive charge finally forms droplet, sputters out from the endpiece of insulation nozzle 14, forms and sprays.Preferably, this endpiece is cone shape.Preferably, the bore of spotting needle insulation nozzle generally is not more than 0.1 millimeter, thereby can reach very high solvent evaporates effect.
Fig. 3 shows the squiggle of the high direct voltage that applies at metal needle tubing 13.By the adjustment of waveform, can guarantee the separation sputter of various drops, make drop slow down again, thereby prolong the volatilization time of solution at the sputtering rate in low level district.The prolongation of solution evaporation time can make more solution evaporation, and this has alleviated the diffusion of point sample band, thereby makes band thinner.Preferably, the repetition frequency of high direct voltage waveform can be set at 500Hz.
More than by example the present invention has been made detailed description, but it should be understood that, foregoing description only has illustrative nature, should not be construed as qualification to the scope of the invention and spirit, for the one of ordinary skilled in the art, on the basis of above-mentioned example, need not can propose various modifications or change through performing creative labour, therefore scope and spirit of the present invention are limited by claim.
Claims (9)
1, a kind of spot sample device is characterized in that, comprises:
The objective table of all product of bearing point;
Spotting arm;
Be installed in the spotting needle on the described spotting arm;
With the motion that described spotting arm links to each other, be used to control described spotting arm or the motion of spotting needle in being parallel and perpendicular to the plane of described stage surface; And
With the high-voltage power module that described spotting needle lotus root is closed, be used between described spotting needle and described point sample sample surfaces, forming high-voltage electric field the spotting solution in the described spotting needle is injected into described point sample sample surfaces.
2, spot sample device as claimed in claim 1, wherein, described spotting needle comprises:
The spotting needle glass tube is used to hold described spotting solution;
Can be along the reciprocating push rod of described spotting needle glass tube inwall;
Insulation nozzle; And
The metal needle tubing that two ends are connected with described spotting needle glass tube and described insulation nozzle respectively, its sidewall closes through lead and described high-voltage power module lotus root.
3, spot sample device as claimed in claim 2, wherein, described insulation nozzle endpiece is cone shape.
4, spot sample device as claimed in claim 2, wherein, the internal diameter of described insulation nozzle is less than 0.1 millimeter.
5, spot sample device as claimed in claim 2, wherein, described insulation nozzle adopting quartz glass is made.
6, spot sample device as claimed in claim 1, wherein, described motion comprises transverse movement mechanism and lengthwise movement mechanism, controls the motion in being parallel and perpendicular to the plane of described stage surface of described spotting arm or spotting needle respectively.
7, spot sample device as claimed in claim 6, wherein, described transverse movement mechanism and lengthwise movement mechanism are screw body.
8, spot sample device as claimed in claim 1 wherein, forms described high-voltage electric field by apply the controlled DC voltage of voltage waveform on described spotting needle.
9, spot sample device as claimed in claim 8, wherein, the repetition frequency of described voltage waveform is 500Hz.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610116183 CN1924574A (en) | 2006-09-19 | 2006-09-19 | Spot sample device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610116183 CN1924574A (en) | 2006-09-19 | 2006-09-19 | Spot sample device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1924574A true CN1924574A (en) | 2007-03-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610116183 Pending CN1924574A (en) | 2006-09-19 | 2006-09-19 | Spot sample device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1924574A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104965047A (en) * | 2014-10-13 | 2015-10-07 | 中国药科大学 | Novel planar chromatography sample application instrument and step repeated sample application method |
| CN111521665A (en) * | 2020-04-09 | 2020-08-11 | 电子科技大学 | Method for regulating and controlling charge quantity and charge property of liquid drops |
-
2006
- 2006-09-19 CN CN 200610116183 patent/CN1924574A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104965047A (en) * | 2014-10-13 | 2015-10-07 | 中国药科大学 | Novel planar chromatography sample application instrument and step repeated sample application method |
| CN111521665A (en) * | 2020-04-09 | 2020-08-11 | 电子科技大学 | Method for regulating and controlling charge quantity and charge property of liquid drops |
| CN111521665B (en) * | 2020-04-09 | 2021-12-21 | 电子科技大学 | Method for regulating and controlling charge quantity and charge property of liquid drops |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20070307 |